CN102369835A - Vegetative propagation method of honeysuckle - Google Patents
Vegetative propagation method of honeysuckle Download PDFInfo
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- CN102369835A CN102369835A CN2011102707348A CN201110270734A CN102369835A CN 102369835 A CN102369835 A CN 102369835A CN 2011102707348 A CN2011102707348 A CN 2011102707348A CN 201110270734 A CN201110270734 A CN 201110270734A CN 102369835 A CN102369835 A CN 102369835A
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Abstract
The invention relates to a vegetative propagation method of honeysuckle. The method comprises cuttage twig preparation, seedling bed preparation, cuttage twig treatment and seedling bed management, wherein the step of cuttage twig preparation comprises cuttage twig selection, cuttage twig shearing and cuttage twig finishing; the seedling bed preparation comprises seedling bed preparation, seedling bed finishing and seedling bed disinfection; the cuttage treatment comprises clearing treatment, cut protection treatment and rooting treatment; and the seedling bed management comprises proper temperature and temperature maintenance, weeding and microelement fertilizer application. The method is applicable to honeysuckle production areas in south of china, such as Sichuan, Chongqing, Hunan, Guizhou and the like. The method is easy to operate, has few process steps and low implementation cost and can be implemented in large scale; in addition, seedling culture cycle is short, only 12-15 months; the survival rate is higher than 85%; the seedling culture cost is reduced by 60%; the quality and impurity of breed are maintained, the effect of zero variation is achieved; disease incidence is reduced to be below 10%.
Description
Technical field
The present invention relates to the asexual reproduction method of plant, be specifically related to a kind of honeysuckle asexual reproduction method.
Background technology
China's honeysuckle main product is in the north; With Shandong, Henan, Hebei and each province, China south is main; Belonging to China's south medicine conventional crude drugs among the people of reaching, also is simultaneously the also a large amount of raw materials of drink enterprise such as China's cold tea, but because of honeysuckle crop cycle long; The kind survival rate is low preferably for quality, and supply falls short of demand to make existing market.
The propagation technique of honeysuckle mainly adopts seed sowing method, the planting of layer and grafting at present: but the seed sowing method is few because of grain weight; Exist seedling to yield poorly; And live seedling is oversize to the florescence growth cycle; Need 4～5 years, during the consumption expenses of labour, so this method is not applied in the breeding of honeysuckle scale at present; The planting of layer is that fresh flower is plucked the later branch in back, and the percutaneous incision layer tissue at the axil place is coated root-growing agent, uses the earth pile things on; Look into situation alive and breed naturally, branch is crawled on ground in implementation process, is not easy to field management such as weeding, fertilising, and shaping is unfavorable for pruning; Make the long untimely pruning of water sprout, influence the growing way of nursery stock, influence output; This method is low because of survival rate, and seedling output is little, and is not promoted; And grafting, the honeysuckle kind that is prone to take root with honeysuckle or largeflower-like honeysuckle flower etc. is as stock, with good honeysuckle kind as scion; Carry out grafting, high through the seedling output of this method breeding, seedling stage to florescence growth cycle is short; Though these methods that adopt of honeysuckle scale breeding at present, the grafting kind of this method is prone to variation, survival rate lower (being about 40%) more; Honeysuckle root disease after triennial is many, and lethality high (being about 30%) has progressively shown its drawback.
Summary of the invention
The object of the present invention is to provide the honeysuckle asexual reproduction method that a kind of growing-seedling period is short, survival rate is high.
The object of the invention specifically is achieved in that a kind of honeysuckle asexual reproduction method, comprises that the cuttage branch is prepared, prepare in the seedbed, the cuttage branch is handled and seedbed management, and its concrete propagation method is following:
1) the cuttage branch is prepared
1.1) select the cuttage branch: in annual 4～September, when face of land temperature rose to 22～25 ℃, clip is annual, and to grow fine, do not have damage by disease and insect and diameter be that the honeysuckle branch of 0.3～0.5cm is as the cuttage branch;
1.2) prune the cuttage branch: the said cuttage branch that will cut was in time pruned the same day; Every is stayed 2 bud heads, and lower end bud head is apart from lower end fracture 2～4cm, and upper end bud head is apart from upper end fracture 4～6cm; Lower end bud head lobe sheet is wiped out fully, upper end bud head lobe sheet is cut 2/3;
1.3) will prune and be bundled into wisp after good cuttage branch is put in order, subsequent use;
2) prepare in the seedbed
2.1) the seedbed selection: select water intaking facility, good, the leeward sandy loam plot that the soil is porous of daylighting as growing seedlings the plot;
2.2) seedbed arrangement: the intertillage plot, training fine earth earth, with 1～1.2 meter wide be a ridge, two ridges are a canopy, little shed is taken with film in every ridge, every canopy is with the sunshade net covering of three pins;
2.3) nursery bed disinfection: use soil disinfectant to spray the seedbed, covered the seedbed 18～24 hours, subsequent use; Said soil disinfectant is preparation like this: will prepare by 1: 600～1: 1000 volume ratio with water and carbendazim, mixing promptly gets;
3) the cuttage branch is handled
3.1) clean
In order to the mass percentage concentration of drinking water preparation be 2～4% and pH value be 8～10 Na
2CO
3Solution is as cleaning agent, will be bundled into the cuttage branch flushing 20～30 minutes of wisp with the mode of circulating water, and is subsequent use;
3.2) the Wound protection processing
To in Wound protective agent, respectively dip in once with cuttage branch two that said cleaning agent washed, the time that each cuttage end of the branch is immersed in the Wound protective agent is no more than 2 seconds, and is subsequent use after handling; Said Wound protective agent is like this preparation: get cold water or distilled water is an amount of; Be heated to 40～50 ℃, add medicinal beta cyclodextrin, make mass percentage concentration and be 3～5% beta cyclodextrin solution after; Add Sodium Benzoate; The mass percentage concentration that makes Sodium Benzoate in the solution is 1～2%, and mixing promptly gets said Wound protective agent;
3.3) processing of taking root
Soaked in root-growing agent 4～8 hours the cuttage branch lower end that will pass through after said Wound protection is handled, and cuttage gets final product; Said root-growing agent is like this preparation: get purity and be 99% heteroauxin 2 grams, with drinking water 20～25L, mixing promptly gets;
4) seedbed management
After the cuttage, keeping seedling bed temperature is 20～35 ℃, and humidity is 75～85%; At any time weed a garden, keep the illumination of appropriateness; After the cuttage 10～15 days, sprayed an amount of trace element fertilizer in per 5～7 days; Sprayed bactericidal agent in per 5～7 days; At any time observe whether damage by disease and insect is arranged, in time handle.
For the ease of the cell division of honeysuckle branch, hestening rooting, the pH value of above-mentioned cleaning agent is preferred 8, the mass percentage concentration of sodium carbonate preferred 4%.
For the cuttage branch wound infection after further preventing to prune, reduce cuttage branch damage by disease and insect and take place, improve the survival rate of cuttage branch, medicinal beta cyclodextrin mass percentage concentration is preferred 5% in the above-mentioned Wound protective agent, Sodium Benzoate mass percentage concentration preferred 1%; The medicinal beta cyclodextrin that said medicinal beta cyclodextrin is preferably produced by the mountains and rivers, Huainan, Anhui pharmaceutic adjuvant Co., Ltd.
Better for the rooting rate of cuttage branch, it is 99% heteroauxin, 2 grams that above-mentioned root-growing agent is preferably got purity, with drinking water 25L preparation, preferred 4 hours of the soak time of cuttage branch in above-mentioned root-growing agent.
For the damage by disease and insect that further reduces the cuttage branch takes place, in the above-mentioned nursery bed disinfection step, preferred 1: 600 of the volume ratio of carbendazim and water, preferred 24 hours of the time that covers the seedbed; Said carbendazim, No. 44, the benzene well imidazoles that the golden the earth agricultural chemicals of preferred Shangqiu City Co., Ltd produces.
For the ease of operation, above-mentioned steps 1.3) in, preferably will prune per 30～50 good of cuttage branch and be bundled into a bundle.
The beneficial effect that the present invention has
Honeysuckle asexual reproduction method of the present invention is suitable for honeysuckle producing regions, China south such as Sichuan, Chongqing, Hunan, Guizhou, and method is easy to operate, and operation is few, and implementation cost is low, but scale is implemented; Growing-seedling period is short, only needs 12～15 months; Survival rate is high, is brought up to more than 85% by existing about 40% survival rate; It is about 60% to greatly reduce seedling cost, is reduced to 2 yuan/seedling by existing month 5 yuan/seedling; The degree of purity that has kept quality and kind, 100% does not make a variation; Reduced the disease rate, probability of happening is reduced to below 10% from existing about 30%.
Embodiment
Through embodiment the present invention is described particularly below; Be necessary to be pointed out that at this; Following examples only are used for the present invention is further explained; Can not be interpreted as the restriction to protection domain of the present invention, the person skilled in the art of this area can make some nonessential improvement and adjustment to the present invention according to the foregoing invention content.
Embodiment 1 implements a kind of honeysuckle asexual reproduction method in the Xiushan Mountain, Chongqing, it comprises, and the cuttage branch is prepared, prepare in the seedbed, the cuttage branch is handled and seedbed management, and its concrete propagation method is following:
1) the cuttage branch is prepared
1.1) select the cuttage branch: in May, when face of land temperature rose to 24 ± 1 ℃, clip is annual, and to grow fine, do not have damage by disease and insect and diameter be that the honeysuckle branch of 0.3～0.5cm is as the cuttage branch;
1.2) prune the cuttage branch: the said cuttage branch that will cut was in time pruned the same day; Every is stayed 2 bud heads, and lower end bud head is apart from lower end fracture 2cm, and upper end bud head is apart from upper end fracture 4cm; Lower end bud head lobe sheet is wiped out fully, upper end bud head lobe sheet is cut 2/3rds;
1.3) will prune and be bundled into wisp after good cuttage branch is put in order, subsequent use;
2) prepare in the seedbed
2.1) the seedbed selection: select water intaking facility, good, the leeward sandy loam plot that the soil is porous of daylighting as growing seedlings the plot;
2.2) seedbed arrangement: the intertillage plot, training fine earth earth, with 1 meter wide be a ridge, two ridges are a canopy, little shed is taken with film in every ridge, every canopy is with the sunshade net covering of three pins;
2.3) nursery bed disinfection: use soil disinfectant to spray the seedbed, covered the seedbed 18 hours, subsequent use; Said soil disinfectant is preparation like this: will prepare by 1: 600 volume ratio with water and carbendazim, mixing promptly gets;
3) the cuttage branch is handled
3.1) clean
In order to the mass percentage concentration of drinking water preparation be 2% and pH value be 8 Na
2CO
3Solution is as cleaning agent, will be bundled into the cuttage branch flushing 30 minutes of wisp with the mode of circulating water, and is subsequent use;
3.2) the Wound protection processing
To in Wound protective agent, respectively dip in once with cuttage branch two that said cleaning agent washed, the time that each cuttage end of the branch is immersed in the Wound protective agent is 1 second, and is subsequent use after handling; Said Wound protective agent is like this preparation: get cold water or distilled water is an amount of; Be heated to 45 ℃, add medicinal beta cyclodextrin, make mass percentage concentration and be 3% beta cyclodextrin solution after; Add Sodium Benzoate; The mass percentage concentration that makes Sodium Benzoate in the solution is 1%, and mixing promptly gets said Wound protective agent;
3.3) processing of taking root
Soaked in root-growing agent 4 hours the cuttage branch lower end that will pass through after said Wound protection is handled, and cuttage gets final product; Said root-growing agent is like this preparation: get purity and be 99% heteroauxin 2 grams, with drinking water 20L, mixing promptly gets;
4) seedbed management
After the cuttage, keeping seedling bed temperature is 20～35 ℃, and humidity is 75～85%; At any time weed a garden, keep the illumination of appropriateness; After the cuttage 10 days, sprayed an amount of trace element fertilizer in per 5 days; Sprayed bactericidal agent in per 5 days; At any time observe whether damage by disease and insect is arranged, in time handle.
The medicinal beta cyclodextrin that this example is used is produced by the mountains and rivers, Huainan, Anhui pharmaceutic adjuvant Co., Ltd; The heteroauxin that this example is used is produced by Chengdu Long March glass reagent Co., Ltd, and the carbendazim that this example is used is by No. 44, the benzene well imidazoles of Shangqiu City gold the earth agricultural chemicals Co., Ltd production.
Honeysuckle through this routine method breeding promptly grows the meat fibrous root after 20 days after the cuttage, seedling raise period is 12 months, and survival rate is high, and 3100 of actual breedings become to live 2685, and survival rate is 86.6%; The disease rate is low, and what disease took place is 281, the about 2 yuan/seedling of seedling cost; Qualitative variation does not take place.
Embodiment 2 implements a kind of honeysuckle asexual reproduction method in the Pengzhou City, Sichuan, comprise that the cuttage branch is prepared, prepare in the seedbed, the cuttage branch is handled and seedbed management, and its concrete propagation method is following:
1) the cuttage branch is prepared
1.1) select the cuttage branch: in annual April, when face of land temperature rose to 22 ℃, clip is annual, and to grow fine, do not have damage by disease and insect and diameter be that the honeysuckle branch of 0.3～0.5cm is as the cuttage branch;
1.2) prune the cuttage branch: the said cuttage branch that will cut was in time pruned the same day; Every is stayed 2 bud heads, and lower end bud head is apart from lower end fracture 4cm, and upper end bud head is apart from upper end fracture 6cm; Lower end bud head lobe sheet is wiped out fully, upper end bud head lobe sheet is cut 2/3rds;
1.3) will prune good cuttage branch and put the back in order and be bundled into wisp by 30 of every bundles, subsequent use;
2) prepare in the seedbed
2.1) the seedbed selection: select water intaking facility, good, the leeward sandy loam plot that the soil is porous of daylighting as growing seedlings the plot;
2.2) seedbed arrangement: the intertillage plot, training fine earth earth, with 1.2 meters wide be a ridge, two ridges are a canopy, little shed is taken with film in every ridge, every canopy is with the sunshade net covering of three pins;
2.3) nursery bed disinfection: use soil disinfectant to spray the seedbed, covered the seedbed 24 hours, subsequent use; Said soil disinfectant is preparation like this: will prepare by 1: 1000 volume ratio with water and carbendazim, mixing promptly gets;
3) the cuttage branch is handled
3.1) clean
In order to the mass percentage concentration of drinking water preparation be 4% and pH value be 10 Na
2CO
3Solution is as cleaning agent, will be bundled into the cuttage branch flushing 30 minutes of wisp with the mode of circulating water, and is subsequent use;
3.2) the Wound protection processing
To in Wound protective agent, respectively dip in once with cuttage branch two that said cleaning agent washed, the time that each cuttage end of the branch is immersed in the Wound protective agent is no more than 2 seconds, and is subsequent use after handling; Said Wound protective agent is like this preparation: get cold water or distilled water is an amount of; Be heated to 40 ℃, add medicinal beta cyclodextrin, make mass percentage concentration and be 5% beta cyclodextrin solution after; Add Sodium Benzoate; The mass percentage concentration that makes Sodium Benzoate in the solution is 2%, and mixing promptly gets said Wound protective agent;
3.3) processing of taking root
Soaked in root-growing agent 8 hours the cuttage branch lower end that will pass through after said Wound protection is handled, and cuttage gets final product; Said root-growing agent is like this preparation: get purity and be 99% heteroauxin 2 grams, with drinking water 25L, mixing promptly gets;
4) seedbed management
After the cuttage, keeping seedling bed temperature is 20～35 ℃, and humidity is 75～85%; At any time weed a garden, keep the illumination of appropriateness; After the cuttage 15 days, sprayed an amount of trace element fertilizer in per 7 days; Sprayed bactericidal agent in per 7 days; At any time observe whether damage by disease and insect is arranged, in time handle.
Honeysuckle through this routine method breeding promptly grows the meat fibrous root after 19 days after the cuttage, seedling raise period is 12 months, and survival rate is high, and 1520 of actual breedings become to live 1325, and survival rate is 89.3%; The disease rate is low, and what disease took place is 124, the about 2 yuan/seedling of seedling cost; Qualitative variation does not take place.
3 one kinds of honeysuckle asexual reproduction methods of embodiment comprise that the cuttage branch is prepared, prepare in the seedbed, the cuttage branch is handled and seedbed management, and its concrete propagation method is following:
1) the cuttage branch is prepared
1.1) select the cuttage branch: in annual September, when face of land temperature rose to 25 ℃, clip is annual, and to grow fine, do not have damage by disease and insect and diameter be that the honeysuckle branch of 0.3～0.5cm is as the cuttage branch;
1.2) prune the cuttage branch: the said cuttage branch that will cut was in time pruned the same day; Every is stayed 2 bud heads, and lower end bud head is apart from lower end fracture 3cm, and upper end bud head is apart from upper end fracture 5cm; Lower end bud head lobe sheet is wiped out fully, upper end bud head lobe sheet is cut 2/3rds;
1.3) will prune good cuttage branch and put the back in order and be bundled into wisp by 50 of every bundles, subsequent use;
2) prepare in the seedbed
2.1) the seedbed selection: select water intaking facility, good, the leeward sandy loam plot that the soil is porous of daylighting as growing seedlings the plot;
2.2) seedbed arrangement: the intertillage plot, training fine earth earth, with 1.1 meters wide be a ridge, two ridges are a canopy, little shed is taken with film in every ridge, every canopy is with the sunshade net covering of three pins;
2.3) nursery bed disinfection: use soil disinfectant to spray the seedbed, covered the seedbed 24 hours, subsequent use; Said soil disinfectant is preparation like this: will prepare by 1: 1000 volume ratio with water and carbendazim, mixing promptly gets;
3) the cuttage branch is handled
3.1) clean
In order to the mass percentage concentration of drinking water preparation be 3% and pH value be 9 Na
2CO
3Solution is as cleaning agent, will be bundled into the cuttage branch flushing 25 minutes of wisp with the mode of circulating water, and is subsequent use;
3.2) the Wound protection processing
To in Wound protective agent, respectively dip in once with cuttage branch two that said cleaning agent washed, the time that each cuttage end of the branch is immersed in the Wound protective agent is no more than 2 seconds, and is subsequent use after handling; Said Wound protective agent is like this preparation: get cold water or distilled water is an amount of; Be heated to 50 ℃, add medicinal beta cyclodextrin, make mass percentage concentration and be 4% beta cyclodextrin solution after; Add Sodium Benzoate; The mass percentage concentration that makes Sodium Benzoate in the solution is 1.5%, and mixing promptly gets said Wound protective agent;
3.3) processing of taking root
Soaked in root-growing agent 6 hours the cuttage branch lower end that will pass through after said Wound protection is handled, and cuttage gets final product; Said root-growing agent is like this preparation: get purity and be 99% heteroauxin 2 grams, with drinking water 22L, mixing promptly gets;
4) seedbed management
After the cuttage, keeping seedling bed temperature is 20～35 ℃, and humidity is 75～85%; At any time weed a garden, keep the illumination of appropriateness; After the cuttage 12 days, sprayed an amount of trace element fertilizer in per 6 days; Sprayed bactericidal agent in per 6 days; At any time observe whether damage by disease and insect is arranged, in time handle.
Honeysuckle through this routine method breeding promptly grew the meat fibrous root in 22 days after the cuttage, and seedling raise period is 13 months, and survival rate is high, and 1640 of actual breedings become to live 1394, and survival rate is 85%; The disease rate is low, and what disease took place is 161, the about 2 yuan/seedling of seedling cost; Qualitative variation does not take place.
Claims (10)
1. a honeysuckle asexual reproduction method comprises that the cuttage branch is prepared, prepare in the seedbed, the cuttage branch is handled and seedbed management, and its concrete propagation method is following:
1) the cuttage branch is prepared
1.1) select the cuttage branch: in annual 4～September, when face of land temperature rose to 22～25 ℃, clip is annual, and to grow fine, do not have damage by disease and insect and diameter be that the honeysuckle branch of 0.3～0.5cm is as the cuttage branch;
1.2) prune the cuttage branch: the said cuttage branch that will cut was in time pruned the same day; Every is stayed 2 bud heads, and lower end bud head is apart from lower end fracture 2～4cm, and upper end bud head is apart from upper end fracture 4～6cm; Lower end bud head lobe sheet is wiped out fully, upper end bud head lobe sheet is cut 2/3;
1.3) will prune and be bundled into wisp after good cuttage branch is put in order, subsequent use;
2) prepare in the seedbed
2.1) the seedbed selection: select water intaking facility, good, the leeward sandy loam plot that the soil is porous of daylighting as growing seedlings the plot;
2.2) seedbed arrangement: the intertillage plot, training fine earth earth, with 1～1.2 meter wide be a ridge, two ridges are a canopy, little shed is taken with film in every ridge, every canopy is with the sunshade net covering of three pins;
2.3) nursery bed disinfection: use soil disinfectant to spray the seedbed, covered the seedbed 18～24 hours, subsequent use; The said earth disinfectant of going up is preparation like this: will prepare by 1: 600～1: 1000 volume ratio with water and carbendazim, mixing promptly gets;
3) the cuttage branch is handled
3.1) clean
In order to the mass percentage concentration of drinking water preparation be 2～4% and pH value be 8～10 Na
2CO
3Solution is as cleaning agent, will be bundled into the cuttage branch flushing 20～30 minutes of wisp with the mode of circulating water, and is subsequent use;
3.2) the Wound protection processing
To in Wound protective agent, respectively dip in once with cuttage branch two that said cleaning agent washed, the time that each cuttage end of the branch is immersed in the Wound protective agent is no more than 2 seconds, and is subsequent use after handling; Said Wound protective agent is like this preparation: get cold water or distilled water is an amount of; Be heated to 40～50 ℃, add medicinal beta cyclodextrin, make mass percentage concentration and be 3～5% beta cyclodextrin solution after; Add Sodium Benzoate; The mass percentage concentration that makes Sodium Benzoate in the solution is 1～2%, and mixing promptly gets said Wound protective agent;
3.3) processing of taking root
Soaked in root-growing agent 4～8 hours the cuttage branch lower end that will pass through after said Wound protection is handled, and cuttage gets final product; Said root-growing agent is like this preparation: get purity and be 99% heteroauxin 2 grams, with drinking water 20～25L, mixing promptly gets;
4) seedbed management
After the cuttage, keeping seedling bed temperature is 20～35 ℃, and humidity is 75～85%; At any time weed a garden, keep the illumination of appropriateness; After the cuttage 10～15 days, sprayed an amount of trace element fertilizer in per 5～7 days; Sprayed bactericidal agent in per 5～7 days; At any time observe whether damage by disease and insect is arranged, in time handle.
2. honeysuckle asexual reproduction method as claimed in claim 1 is characterized in that: the pH value of said cleaning agent is 8, and the mass percentage concentration of sodium carbonate is 4%.
3. according to claim 1 or claim 2 honeysuckle asexual reproduction method, it is characterized in that: the mass percentage concentration of medicinal beta cyclodextrin described in the said Wound protective agent is 5%, the mass percentage concentration of said Sodium Benzoate is 1%.
4. according to claim 1 or claim 2 honeysuckle asexual reproduction method is characterized in that: the medicinal beta cyclodextrin of said medicinal beta cyclodextrin for being produced by the mountains and rivers, Huainan, Anhui pharmaceutic adjuvant Co., Ltd.
5. honeysuckle asexual reproduction method as claimed in claim 3 is characterized in that: the medicinal beta cyclodextrin of said medicinal beta cyclodextrin for being produced by the mountains and rivers, Huainan, Anhui pharmaceutic adjuvant Co., Ltd.
6. according to claim 1 or claim 2 honeysuckle asexual reproduction method is characterized in that: said root-growing agent is 99% heteroauxin 2 grams by purity, and 25L is formulated with drinking water, and the cuttage branch soaked 4 hours in said root-growing agent.
7. honeysuckle asexual reproduction method as claimed in claim 3 is characterized in that: said root-growing agent is 99% heteroauxin, 2 grams by purity, and 25L is formulated with drinking water, and the cuttage branch soaked 4 hours in said root-growing agent.
8. according to claim 1 or claim 2 honeysuckle asexual reproduction method, it is characterized in that: in the said nursery bed disinfection step, the volume ratio of carbendazim and water is 1: 600, covers the seedbed 24 hours.
9. honeysuckle asexual reproduction method as claimed in claim 3 is characterized in that: in the said nursery bed disinfection step, the volume ratio of carbendazim and water is 1: 600, covers the seedbed 24 hours.
10. like claim 6 or 7 described honeysuckle asexual reproduction methods, it is characterized in that: in the said nursery bed disinfection step, the volume ratio of carbendazim and water is 1: 600, covers the seedbed 24 hours.
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| CN105210635A (en) * | 2015-10-13 | 2016-01-06 | 广西乐业荣丰中药材科技有限责任公司 | A kind of honeysuckle cuttage and seedling culture method |
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| CN105393782A (en) * | 2015-12-03 | 2016-03-16 | 广西佳联达生态农业发展有限责任公司 | Chrysanthemum nankingense plantation method |
| CN107155556A (en) * | 2017-04-28 | 2017-09-15 | 阮昌文 | A kind of honeysuckle propagation method |
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