CN102630444A - Non-test-tube rapid propagation technology of plants - Google Patents
Non-test-tube rapid propagation technology of plants Download PDFInfo
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- CN102630444A CN102630444A CN2012100783939A CN201210078393A CN102630444A CN 102630444 A CN102630444 A CN 102630444A CN 2012100783939 A CN2012100783939 A CN 2012100783939A CN 201210078393 A CN201210078393 A CN 201210078393A CN 102630444 A CN102630444 A CN 102630444A
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Abstract
The invention relates to the technical field of vegetative propagation of plants and in particular relates a non-test-tube rapid propagation technology of the plants. The non-test-tube rapid propagation technology particularly comprises the following steps of: simultaneously establishing water supply and temperature control systems while establishing a rapid propagation seedling bed; and utilizing matched facilities including a time relay or an interval time controller, a temperature instrument and the like to establish a good growth environment for plant separation materials cultivated in the seedling bed. The facilities have low cost and can precisely control temperature and humidity of the seedling growing environment, so that the aim that the plant separation materials cultivated in the seedling bed can rapidly take roots and grow into seedlings is realized. The non-test-tube rapid propagation technology of the plants has the advantages of simple process, less equipment investment, high automation degree, accurate management and control, wide rapid propagation varieties and low seedling propagation cost, and can realize rapid annular propagation and is easy to popularize and spread.
Description
Technical field
The present invention relates to the vegetative technical field of a plant species, be specifically related to the non-tube rapid propagation technology of a plant species.
Background technology
At present, the approach of plant propagation is mainly through sexual propagation and two kinds of methods of vegetative propagation.Vegetative propagation then mainly is traditional cuttage and seedling culture and tissue culture.
Traditional cuttage raise seedling technique is simple for process, but should technology in final-period management, operates very loaded down with trivial detailsly, and this technology has that growing-seedling period is long, planting percent is low, seasonality is strong simultaneously, and many plants are difficult to through shortcomings such as cuttage raise seedling technique breedings.
Plant Tissue Breeding is a plant cell engineering technology of rising in 20th century.It all has " totipotency " that form this plant corpus ability theoretical and development according to any one plant cell; Take off any one, several cell or a block organization from plant corpus; Under all by manual work construction particular environment condition, cultivate; Through cell division and tissue differentiation, reach plant forms and rebuild and quick breeding purpose.But plant tissue culture technique is that the easy biography property of a technical very strong job, technology is poor, and infrastructure has high input, and aseptic condition requires high, and the refining seedling cycle is long, and the very difficult penetration and promotion of this technology is used.
Non-tube rapid propagation technology is a brand-new plant fast propagation seedling growing process, and it has abandoned shortcomings such as the high investment of tissue culture, difficult operation, easy infection, refining Miao Nan, has propagating materials to economize the reproduction coefficient advantages of higher again equally.
The non-tube rapid propagation technical matters of plant of Lishui of Zhejiang institute of agricultural sciences exploitation is temperature, a humidity of utilizing calculator to control environment, reaches the purpose of quick breeding to the stripped material good environment of construction of plant.This technology in implementation process applied core computer its involve great expense, equipment use complicated, later maintenance is difficult, technical application is professional, the ordinary people is difficult to grasp.
Application number is 200610017711.5 " kind of plant half bud or a leaf method of quick propagating and cultivating "; This technology is to adopt half tender shoots or a blade of giving birth to the xylophyta of semi-lignified then; Perhaps adopt the tender stem of blade-carrying herbaceous plant to make plugged ear, slotting machine is gone in plugged ear cuttage after bactericidal liquid, root-inducing powder are handled; Spray immediately after the cuttage 1 time permeable, get into control spraying management automatically then; Spray the bactericidal liquid morbidity of avoiding infection in morning next day or evening, spray bactericidal liquid afterwards at interval; The back sprinkling phosphate fertilizer that begins to take root promotes the root system lignifying; Got regeneration plant through 4～11 days.This technical matters is simple, but its described is adopted a blade to be difficult to realize obtaining regeneration plant by this technical matters.In addition, just control spraying management automatically that this technical matters designed, but the temperature control measure can not put in place during low temperature, is difficult to realize the annual breeding.
Summary of the invention
The object of the invention is exactly to overcome the deficiency of above-mentioned technology and the non-tube rapid propagation technology of brand-new plant species technology is provided.Specifically comprise: set up water supply, temperature-controlling system simultaneously setting up the fast propagating seedling bed, utilize the time relay or auxiliary facilities such as intermittent time controller, thermometric instrument to build a good growing environment for the seedbed plant that the inside the is cultivated material that exsomatizes.These facilities are cheap, but can realize critically controlling temperature, the humidity of the growing environment of growing seedlings, and are placed on the plant of cultivating the inside, the seedbed material that exsomatizes and reach the purpose of taking root into seedling fast thereby make.
The present invention realizes through following technical scheme:
1, the non-tube rapid propagation technology of a plant species; Comprise in the technical scheme: the structure of fast propagating seedling bed, the control water system in seedbed and the foundation of temperature-controlling system, the selecting for use and preparing of hormone; The collection and the preparation method of the stripped material of plant; The plant material that exsomatizes soaks hormone and sterilization method, and the plant material that exsomatizes inserts the seedbed method, and plant is exsomatized material in management during the seedbed and the trasplanting method after taking root.
2, the non-tube rapid propagation technology of a plant species, the construction method in seedbed is following in the technical scheme:
(1) selective light is according to sufficient, and the seedbed is built on the good ground of draining, and the seedbed specification is built furrow by long 10～15m, wide 1～1.2m, and the furrow limit is an edge of bed of building the high 15～20cm of one-tenth with brick, stays the slit in order to draining between brick and the brick slightly, stays the aisle of 40～50cm between the seedbed;
(2) medium of seedling bed is to be used for fixing the stripped material of plant, and choice of base can be suited measures to local conditions, and gathers materials on the spot, and can be perlite, river sand, vermiculite, cinder, sawdust.
3, the non-tube rapid propagation technology of a plant species, the water system method for building up in seedbed is following in the technical scheme:
(1) micro-nozzle road on frame above the center, seedbed, the micro-nozzle road is 60～70cm far from bed surface, and every interval 40～50cm installs an atomizer on the pipeline, and the direction that shower nozzle is installed is vertically upward;
(2) the micro-nozzle road is connecting water pump, during work is to be supplied water by water pump, and fills the air at air from atomizer ejection and formation water atomization shape through pipeline under the pressure that water produces when water pump supplies water, thereby reach needed humidity requirement in the environment of growing seedlings;
(3) water pump is to be controlled by the time relay or intermittent time controller; The effect of the time relay or intermittent time controller is when to spray water for configuring through the people; After spraying the such data of water how long, the time relay or intermittent time controller will be that the parameter that sets comes control of pump to carry out work and water spray according to the people;
(4) establish tank at the head of a bed in seedbed, water pump is placed in the tank.
4, the non-tube rapid propagation technology of a plant species, it is following that the seedbed supplies the method for building up of warm system in the technical scheme:
(1) supply warm system to form by thermometric instrument, temp probe, heater wire;
(2) heater wire is that temp probe is the acquisition environment temperature, and at any time environmental temperature is fed back to thermometric instrument by thermometric instrument control; Heater wire is laid on the matrix the inside in seedbed, and temp probe also is inserted in the matrix in seedbed; After thermometric instrument configured the ambient temperature data of required control, when environmental temperature was lower than the temperature of artificial setting, thermometric instrument started heater wire through artificial; After the heater wire heating, heat spreads in the environmental field of being controlled, when environmental temperature reaches the temperature of artificial setting; Thermometric instrument cuts off the power supply automatically; When environmental temperature was lower than the temperature of artificial setting again, thermometric instrument can start heater wire again automatically, is controlled at environmental temperature in the artificial range of set temperature all the time.
5, the non-tube rapid propagation of plant species technology, in the technical scheme hormone select for use and compound method following:
(1) selecting for use of hormone: can accelerate the generation of callus formation and root original hase after the otch of the stripped material of plant is handled with somatotropin; The amount of taking root also has tangible growth; Particularly take root or extremely difficult breeding kind to difficulty, drug-induced have very important; Hormone commonly used has: methyl, heteroauxin, indolebutyric acid, ABT1～No. 6;
(2) preparation of hormone: the stripped material that is applied to different kinds and different size according to different hormone is prepared the soup of variable concentrations; Difficulty is dissolved in the hormone of water, earlier with a certain amount of medicinal alcohol come rare after, add clear water again and dissolve extremely required concentration surely.
6, the non-tube rapid propagation technology of a plant species, the collection and the preparation method of the stripped material of plant are following in the technical scheme:
(A) In vitro plant material collection: Select vigorous growth, no pests of trees as the female parent; difficult breeding species selection for the young and strong as in vitro plant material, and try to choose a semi-woody branches or slightly growing strong or stem segments as in vitro material, because these little sticks or stems physiology and metabolism, is conducive to wound healing and root formation, root seedling faster;
(2) exsomatize the time period acquired for materials: plant will be arranged in morning, shady and cool time period in evening or overcast and rainy whole day when stripped acquired for materials;
(3) making of the stripped material of plant: adopt sharp pruning shear, grafting knife or blade; Be cut into the branch section of a leaf one bud or polygerm with cutter; Cut otch with blade etc. smoothly again, have like this in the formation of callus and root original hase, otch should be near eye and the inclined-plane cut; Because nutrition is more concentrated, help the formation of callus and root original hase here; Stripped material will stay a blade at least, and this is crucial, but for the plant that fall leaves winter, need not be with leaf, because of backflow Nutrient Accumulation before the existing blade fallen leaves in the branch section, can satisfy the nutritional need of taking root.
7, the non-tube rapid propagation technology of a plant species, plant is exsomatized in the technical scheme, and material soaks hormone and disinfectant method is following:
(1) plant is exsomatized material with base portion down, cutting is classified good and is lashed by size, and neat concentrated being placed in the plastic basin poured the hormone for preparing in the basin into to flooding the cutting base portion;
(2) according to the diameter of transplanting a cutting, and hormone concentration is decided soak time;
(3) not infected for the wound that makes the material that exsomatizes, carry out disinfection according to the otch of desired concn interpolation bactericide in the time of configuration hormone soup to the stripped material of plant.
8, the non-tube rapid propagation of plant species technology, plant is exsomatized material when inserting the seedbed in the technical scheme, has the plant of the leaf material that exsomatizes heavily not build with blade and be as the criterion, and the stripped material of the stem plant of no leaf evenly inserts the seedbed by the spacing of 2～3cm between strain and the strain.
9, the non-tube rapid propagation of plant species technology, in the technical scheme plant exsomatize material in management during the seedbed and the trasplanting method after taking root following:
(1) water spray is managed: in a single day the stripped material of plant is cut behind female parent, and the moisture of stripped material, nutrient supply are also disconnected in succession, and after material was inserted into medium of seedling bed so plant is exsomatized, the branch body and the blade of spraying with the stripped material of assurance plant that be about to begin kept moistening; The exsomatize mist frequency of callus spraying before forming of material of plant is per 1: 30 hour water spray 10～15 seconds; The frequency of spraying after the generation of the root original hase of the stripped material of plant changes per 3 hours into and sprayed water 8～10 seconds; The longer back spraying of the coring of the stripped material of plant frequency changes per 5 hours into and sprayed water 8～10 seconds, to evening or the overcast and rainy work that can stop to control water system;
(2) temperature treatment: when at the bottom of the environmental temperature during, start the warm system that supplies, environmental temperature is set and is controlled at 22～25 ℃ in 18 ℃;
(3) foliar application: after the generation of the root original hase of the stripped material of plant, spraying concentration is 0.2～0.3% biphosphate potassium foliage fertilizer, and spray in per 7 days once becomes seedling and transplanting until the stripped material of plant;
(4) nursery bed disinfection: plant is exsomatized after material is inserted into the seedbed, every at a distance from 7 days between the lights the period be that 800 times carbendazim soup thimerosal carries out disinfection after stopping to control the work of water system to the seedbed spray concentration, become seedling and transplanting until the plant material that exsomatizes;
(5) transplanting of taking root: exsomatize after the base portion of material grows square root when plant, just can transplant the stripped material of plant to field production.
Compared with prior art, the existing following advantage of the present invention:
1, the seedling raising process of the present invention in implementation process is to utilize the time relay or supporting control device such as intermittent time controller, thermometric instrument; Optimal parameter through these control devices are set commands water spray atomizing, temperature control to wait temperature, the humidity environment of controlling the fast propagating seedling bed, builds a good growing environment for the stripped material of the fast propagating seedling bed plant that the inside is cultivated; These facilities are cheap, but can realize the growing environment that accurate control is grown seedlings, and make whole seedling raising process realize automation, save a large amount of management recruitments and also increase work efficiency simultaneously.
2, realized the rapid propagation in factory new technology of simple and easy automation, batch production, anniversaryization through embodiment of the present invention.
3, during embodiment of the present invention, technical application property is strong, under simple and easy condition, makes ordinary person's easy master, easy to operate, has strengthened the easy biography property of fast breeding technique and popular.
4, during embodiment of the present invention, only get a leaf one bud of plant in the operation, general plant can take root through 7～15 days cultivation time period and form another complete plant; The seedling of breeding out strengthens than the adaptability of tissue-culturing rapid propagation greatly, has overcome group and has cultivated the difficult technical barrier of seedling refining seedling.
5, the invention belongs to vegetative propagation, on gene genetic, belong to stable type, proterties sudden change and variation can not take place.
6, through embodiment of the present invention, seedling cost numerous soon, that produce is low, is flexible in management; As: seedlings such as various places woods fruit flowers, vegetables medicinal material update fast; These new, special products of famous brands, of extra fine quality kinds are under the situation of no seedling, and fast breeding technique more shows its enchanting charm, find the rare kind what is good; Only need take back some cauline leaf branches or buy that one or two young plant returns can be numerous soon in a large number, and one need not spend high price to introduce a fine variety in a large number; Two can the popularization of high price batch obtain high profit again.
Embodiment
Below in conjunction with embodiment method of the present invention is further specified.
The non-tube rapid propagation technology of one plant species, embodiment is following:
1, the non-tube rapid propagation technology of a plant species; Comprise in the technical scheme: the structure of fast propagating seedling bed, the control water system in seedbed and the foundation of temperature-controlling system, the selecting for use and preparing of hormone; The collection and the preparation method of the stripped material of plant; The plant material that exsomatizes soaks hormone and sterilization method, and the plant material that exsomatizes inserts the seedbed method, and plant is exsomatized material in management during the seedbed and the trasplanting method after taking root.
2, the non-tube rapid propagation technology of a plant species, the construction method in seedbed is following in the technical scheme:
(1) selective light is according to sufficient, and the seedbed is built on the good ground of draining, and the seedbed specification is built furrow by long 10～15m, wide 1～1.2m, and the furrow limit is an edge of bed of building the high 15～20cm of one-tenth with brick, stays the slit in order to draining between brick and the brick slightly, stays the aisle of 40～50cm between the seedbed;
(2) medium of seedling bed is to be used for fixing the stripped material of plant, and choice of base can be suited measures to local conditions, and gathers materials on the spot, and can be perlite, river sand, vermiculite, cinder, sawdust.
3, the non-tube rapid propagation technology of a plant species, the water system method for building up in seedbed is following in the technical scheme:
(1) micro-nozzle road on frame above the center, seedbed, the micro-nozzle road is 60～70cm far from bed surface, and every interval 40～50cm installs an atomizer on the pipeline, and the direction that shower nozzle is installed is vertically upward;
(2) the micro-nozzle road is connecting water pump, during work is to be supplied water by water pump, and fills the air at air from atomizer ejection and formation water atomization shape through pipeline under the pressure that water produces when water pump supplies water, thereby reach needed humidity requirement in the environment of growing seedlings;
(3) water pump is to be controlled by the time relay or intermittent time controller; The effect of the time relay or intermittent time controller is when to spray water for configuring through the people; After spraying the such data of water how long, the time relay or intermittent time controller will be that the parameter that sets comes control of pump to carry out work and water spray according to the people;
(4) establish tank at the head of a bed in seedbed, water pump is placed in the tank.
4, the non-tube rapid propagation technology of a plant species, it is following that the seedbed supplies the method for building up of warm system in the technical scheme:
(1) supply warm system to form by thermometric instrument, temp probe, heater wire;
(2) heater wire is that temp probe is the acquisition environment temperature, and at any time environmental temperature is fed back to thermometric instrument by thermometric instrument control; Heater wire is laid on the matrix the inside in seedbed, and temp probe also is inserted in the matrix in seedbed; After thermometric instrument configured the ambient temperature data of required control, when environmental temperature was lower than the temperature of artificial setting, thermometric instrument started heater wire through artificial; After the heater wire heating, heat spreads in the environmental field of being controlled, when environmental temperature reaches the temperature of artificial setting; Thermometric instrument cuts off the power supply automatically; When environmental temperature was lower than the temperature of artificial setting again, thermometric instrument can start heater wire again automatically, is controlled at environmental temperature in the artificial range of set temperature all the time.
5, the non-tube rapid propagation of plant species technology, in the technical scheme hormone select for use and compound method following:
(1) selecting for use of hormone: can accelerate the generation of callus formation and root original hase after the otch of the stripped material of plant is handled with somatotropin; The amount of taking root also has tangible growth; Particularly take root or extremely difficult breeding kind to difficulty, drug-induced have very important; Hormone commonly used has: methyl, heteroauxin, indolebutyric acid, ABT1～No. 6;
(2) preparation of hormone: the stripped material that is applied to different kinds and different size according to different hormone is prepared the soup of variable concentrations; Difficulty is dissolved in the hormone of water, earlier with a certain amount of medicinal alcohol come rare after, add clear water again and dissolve extremely required concentration surely.
6, the non-tube rapid propagation technology of a plant species, the collection and the preparation method of the stripped material of plant are following in the technical scheme:
(A) In vitro plant material collection: Select vigorous growth, no pests of trees as the female parent; difficult breeding species selection for the young and strong as in vitro plant material, and try to choose a semi-woody branches or slightly growing strong or stem segments as in vitro material, because these little sticks or stems physiology and metabolism, is conducive to wound healing and root formation, root seedling faster;
(2) exsomatize the time period acquired for materials: plant will be arranged in morning, shady and cool time period in evening or overcast and rainy whole day when stripped acquired for materials;
(3) making of the stripped material of plant: adopt sharp pruning shear, grafting knife or blade; Be cut into the branch section of a leaf one bud or polygerm with cutter; Cut otch with blade etc. smoothly again, have like this in the formation of callus and root original hase, otch should be near eye and the inclined-plane cut; Because nutrition is more concentrated, help the formation of callus and root original hase here; Stripped material will stay a blade at least, and this is crucial, but for the plant that fall leaves winter, need not be with leaf, because of backflow Nutrient Accumulation before the existing blade fallen leaves in the branch section, can satisfy the nutritional need of taking root.
7, the non-tube rapid propagation technology of a plant species, plant is exsomatized in the technical scheme, and material soaks hormone and disinfectant method is following:
(1) plant is exsomatized material with base portion down, cutting is classified good and is lashed by size, and neat concentrated being placed in the plastic basin poured the hormone for preparing in the basin into to flooding the cutting base portion;
(2) according to the diameter of transplanting a cutting, and hormone concentration is decided soak time;
(3) not infected for the wound that makes the material that exsomatizes, carry out disinfection according to the otch of desired concn interpolation bactericide in the time of configuration hormone soup to the stripped material of plant.
8, the non-tube rapid propagation of plant species technology, plant is exsomatized material when inserting the seedbed in the technical scheme, has the plant of the leaf material that exsomatizes heavily not build with blade and be as the criterion, and the stripped material of the stem plant of no leaf evenly inserts the seedbed by the spacing of 2～3cm between strain and the strain.
9, the non-tube rapid propagation of plant species technology, in the technical scheme plant exsomatize material in management during the seedbed and the trasplanting method after taking root following:
(1) water spray is managed: in a single day the stripped material of plant is cut behind female parent, and the moisture of stripped material, nutrient supply are also disconnected in succession, and after material was inserted into medium of seedling bed so plant is exsomatized, the branch body and the blade of spraying with the stripped material of assurance plant that be about to begin kept moistening; The exsomatize mist frequency of callus spraying before forming of material of plant is per 1: 30 hour water spray 10～15 seconds; The frequency of spraying after the generation of the root original hase of the stripped material of plant changes per 3 hours into and sprayed water 8～10 seconds; The longer back spraying of the coring of the stripped material of plant frequency changes per 5 hours into and sprayed water 8～10 seconds, to evening or the overcast and rainy work that can stop to control water system;
(2) temperature treatment: when at the bottom of the environmental temperature during, start the warm system that supplies, environmental temperature is set and is controlled at 22～25 ℃ in 18 ℃;
(3) foliar application: after the generation of the root original hase of the stripped material of plant, spraying concentration is 0.2～0.3% biphosphate potassium foliage fertilizer, and spray in per 7 days once becomes seedling and transplanting until the stripped material of plant;
(4) nursery bed disinfection: plant is exsomatized after material is inserted into the seedbed, every at a distance from 7 days between the lights the period be that 800 times carbendazim soup thimerosal carries out disinfection after stopping to control the work of water system to the seedbed spray concentration, become seedling and transplanting until the plant material that exsomatizes;
(5) transplanting of taking root: exsomatize after the base portion of material grows square root when plant, just can transplant the stripped material of plant to field production.
Claims (9)
1. the non-tube rapid propagation of a plant species is technological; Its characteristic comprises: the structure of fast propagating seedling bed, the control water system in seedbed and the foundation of temperature-controlling system, the selecting for use and preparing of hormone; The collection and the preparation method of the stripped material of plant; The plant material that exsomatizes soaks hormone and sterilization method, and the plant material that exsomatizes inserts the seedbed method, and plant is exsomatized material in management during the seedbed and the trasplanting method after taking root.
2. the non-tube rapid propagation technology of a plant species according to claim 1 is characterized in that the construction method in seedbed is following:
(1) selective light is according to sufficient, and the seedbed is built on the good ground of draining, and the seedbed specification is built furrow by long 10～15m, wide 1～1.2m, and the furrow limit is an edge of bed of building the high 15～20cm of one-tenth with brick, stays the slit in order to draining between brick and the brick slightly, stays the aisle of 40～50cm between the seedbed;
(2) medium of seedling bed is to be used for fixing the stripped material of plant, and choice of base can be suited measures to local conditions, and gathers materials on the spot, and can be perlite, river sand, vermiculite, cinder, sawdust.
3. the non-tube rapid propagation technology of a plant species according to claim 1 is characterized in that the water system method for building up in seedbed is following:
(1) micro-nozzle road on frame above the center, seedbed, the micro-nozzle road is 60～70cm far from bed surface, and every interval 40～50cm installs an atomizer on the pipeline, and the direction that shower nozzle is installed is vertically upward;
(2) the micro-nozzle road is connecting water pump, during work is to be supplied water by water pump, and fills the air at air from atomizer ejection and formation water atomization shape through pipeline under the pressure that water produces when water pump supplies water, thereby reach needed humidity requirement in the environment of growing seedlings;
(3) water pump is to be controlled by the time relay or intermittent time controller; The effect of the time relay or intermittent time controller is when to spray water for configuring through the people; After spraying the such data of water how long, the time relay or intermittent time controller will be that the parameter that sets comes control of pump to carry out work and water spray according to the people;
(4) establish tank at the head of a bed in seedbed, water pump is placed in the tank.
4. the non-tube rapid propagation technology of a plant species according to claim 1 is characterized in that the seedbed supplies the method for building up of warm system following:
(1) supply warm system to form by thermometric instrument, temp probe, heater wire;
(2) heater wire is that temp probe is the acquisition environment temperature, and at any time environmental temperature is fed back to thermometric instrument by thermometric instrument control; Heater wire is laid on the matrix the inside in seedbed, and temp probe also is inserted in the matrix in seedbed; After thermometric instrument configured the ambient temperature data of required control, when environmental temperature was lower than the temperature of artificial setting, thermometric instrument started heater wire through artificial; After the heater wire heating, heat spreads in the environmental field of being controlled, when environmental temperature reaches the temperature of artificial setting; Thermometric instrument cuts off the power supply automatically; When environmental temperature was lower than the temperature of artificial setting again, thermometric instrument can start heater wire again automatically, is controlled at environmental temperature in the artificial range of set temperature all the time.
5. the non-tube rapid propagation of a plant species according to claim 1 technology, it is characterized in that hormone select for use and compound method following:
(1) selecting for use of hormone: can accelerate the generation of callus formation and root original hase after the otch of the stripped material of plant is handled with somatotropin; The amount of taking root also has tangible growth; Particularly take root or extremely difficult breeding kind to difficulty, drug-induced have very important; Hormone commonly used has: methyl, heteroauxin, indolebutyric acid, ABT1～No. 6;
(2) preparation of hormone: the stripped material that is applied to different kinds and different size according to different hormone is prepared the soup of variable concentrations; Difficulty is dissolved in the hormone of water, earlier with a certain amount of medicinal alcohol come rare after, add clear water again and dissolve extremely required concentration surely.
6. the non-tube rapid propagation technology of a plant species according to claim 1 is characterized in that the collection and the preparation method of the stripped material of plant is following:
(A) In vitro plant material collection: Select vigorous growth, no pests of trees as the female parent; difficult breeding species selection for the young and strong as in vitro plant material, and try to choose a semi-woody branches or slightly growing strong or stem segments as in vitro material, because these little sticks or stems physiology and metabolism, is conducive to wound healing and root formation, root seedling faster;
(2) exsomatize the time period acquired for materials: plant will be arranged in morning, shady and cool time period in evening or overcast and rainy whole day when stripped acquired for materials;
(3) making of the stripped material of plant: adopt sharp pruning shear, grafting knife or blade; Be cut into the branch section of a leaf one bud or polygerm with cutter; Cut otch with blade etc. smoothly again, have like this in the formation of callus and root original hase, otch should be near eye and the inclined-plane cut; Because nutrition is more concentrated, help the formation of callus and root original hase here; Stripped material will stay a blade at least, and this is crucial, but for the plant that fall leaves winter, need not be with leaf, because of backflow Nutrient Accumulation before the existing blade fallen leaves in the branch section, can satisfy the nutritional need of taking root.
7. the non-tube rapid propagation technology of a plant species according to claim 1, material soaks hormone and disinfectant method is following to it is characterized in that exsomatizing plant:
(1) plant is exsomatized material with base portion down, cutting is classified good and is lashed by size, and neat concentrated being placed in the plastic basin poured the hormone for preparing in the basin into to flooding the cutting base portion;
(2) according to the diameter of transplanting a cutting, and hormone concentration is decided soak time;
(3) not infected for the wound that makes the material that exsomatizes, carry out disinfection according to the otch of desired concn interpolation bactericide in the time of configuration hormone soup to the stripped material of plant.
8. the non-tube rapid propagation technology of a plant species according to claim 1; When it is characterized in that plant is exsomatized material insertion seedbed; Have the plant of the leaf material that exsomatizes heavily not build with blade and be as the criterion, the stripped material of the stem plant of no leaf evenly inserts the seedbed by the spacing of 2～3cm between strain and the strain.
9. the non-tube rapid propagation of a plant species according to claim 1 technology, it is characterized in that plant exsomatize material in management during the seedbed and the trasplanting method after taking root following:
(1) water spray is managed: in a single day the stripped material of plant is cut behind female parent, and the moisture of stripped material, nutrient supply are also disconnected in succession, and after material was inserted into medium of seedling bed so plant is exsomatized, the branch body and the blade of spraying with the stripped material of assurance plant that be about to begin kept moistening; The exsomatize mist frequency of callus spraying before forming of material of plant is per 1: 30 hour water spray 10～15 seconds; The frequency of spraying after the generation of the root original hase of the stripped material of plant changes per 3 hours into and sprayed water 8～10 seconds; The longer back spraying of the coring of the stripped material of plant frequency changes per 5 hours into and sprayed water 8～10 seconds, to evening or the overcast and rainy work that can stop to control water system;
(2) temperature treatment: when at the bottom of the environmental temperature during, start the warm system that supplies, environmental temperature is set and is controlled at 22～25 ℃ in 18 ℃;
(3) foliar application: after the generation of the root original hase of the stripped material of plant, spraying concentration is 0.2～0.3% biphosphate potassium foliage fertilizer, and spray in per 7 days once becomes seedling and transplanting until the stripped material of plant;
(4) nursery bed disinfection: plant is exsomatized after material is inserted into the seedbed, every at a distance from 7 days between the lights the period be that 800 times carbendazim soup thimerosal carries out disinfection after stopping to control the work of water system to the seedbed spray concentration, become seedling and transplanting until the plant material that exsomatizes;
(5) transplanting of taking root: exsomatize after the base portion of material grows square root when plant, just can transplant the stripped material of plant to field production.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100783939A CN102630444A (en) | 2011-12-10 | 2012-03-12 | Non-test-tube rapid propagation technology of plants |
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| CN103081791A (en) * | 2013-02-05 | 2013-05-08 | 镇江市丹徒区紫杉生态农业园 | Nutrient solution cultivation method of Chinese yews |
| CN103880564A (en) * | 2014-03-11 | 2014-06-25 | 何国森 | Preparation method of rhododendron culture medium |
| CN104285767A (en) * | 2014-09-28 | 2015-01-21 | 赵青娇 | Rapid seedling growing method for red cluster peppers |
| CN106888715A (en) * | 2015-07-22 | 2017-06-27 | 全南厚朴生态林业有限公司 | A kind of color Ye Gui a, famous physician of the Qing Dynasty flower ' purple handsome princess ' non-line-of-sight error method |
| CN107018900A (en) * | 2017-04-26 | 2017-08-08 | 中国科学院合肥物质科学研究院 | A kind of method of hydrangea plant fast breeding |
| CN107047262A (en) * | 2017-06-05 | 2017-08-18 | 四川格睿园林科技有限公司 | A kind of non-test tube seedling method of quick propagating and cultivating of Cotinus coggygria |
| CN107360888A (en) * | 2017-07-19 | 2017-11-21 | 福建师范大学 | A kind of seedling-raising technique for improving Common zenia cuttage survival rate |
| CN109197191A (en) * | 2018-09-07 | 2019-01-15 | 田东县文设芒果专业合作社 | A kind of propagation method of mango seedling |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103081791A (en) * | 2013-02-05 | 2013-05-08 | 镇江市丹徒区紫杉生态农业园 | Nutrient solution cultivation method of Chinese yews |
| CN103880564A (en) * | 2014-03-11 | 2014-06-25 | 何国森 | Preparation method of rhododendron culture medium |
| CN103880564B (en) * | 2014-03-11 | 2016-03-30 | 何国森 | The preparation method of rhododendron cultivation matrix |
| CN104285767A (en) * | 2014-09-28 | 2015-01-21 | 赵青娇 | Rapid seedling growing method for red cluster peppers |
| CN106888715A (en) * | 2015-07-22 | 2017-06-27 | 全南厚朴生态林业有限公司 | A kind of color Ye Gui a, famous physician of the Qing Dynasty flower ' purple handsome princess ' non-line-of-sight error method |
| CN107018900A (en) * | 2017-04-26 | 2017-08-08 | 中国科学院合肥物质科学研究院 | A kind of method of hydrangea plant fast breeding |
| CN107047262A (en) * | 2017-06-05 | 2017-08-18 | 四川格睿园林科技有限公司 | A kind of non-test tube seedling method of quick propagating and cultivating of Cotinus coggygria |
| CN107360888A (en) * | 2017-07-19 | 2017-11-21 | 福建师范大学 | A kind of seedling-raising technique for improving Common zenia cuttage survival rate |
| CN109197191A (en) * | 2018-09-07 | 2019-01-15 | 田东县文设芒果专业合作社 | A kind of propagation method of mango seedling |
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