CN114652765A - Natural plant glycolysis product for preventing and controlling enteritis of pigs as well as preparation method and application thereof - Google Patents
Natural plant glycolysis product for preventing and controlling enteritis of pigs as well as preparation method and application thereof Download PDFInfo
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- A61K35/744—Lactic acid bacteria, e.g. enterococci, pediococci, lactococci, streptococci or leuconostocs
- A61K35/747—Lactobacilli, e.g. L. acidophilus or L. brevis
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A61K2236/10—Preparation or pretreatment of starting material
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Abstract
The invention discloses a natural plant glycolysis product for preventing and controlling enteritis of pigs, which comprises the following raw materials in parts by weight: 5-10 parts of lactobacillus rhamnosus, 5-10 parts of lactobacillus plantarum, 15-25 parts of coptis chinensis, 15-20 parts of bighead atractylodes rhizome, 10-15 parts of fructus forsythiae and 25-35 parts of astragalus membranaceus; the preparation method comprises the following steps: (1) weighing the raw materials; (2) mixing lactobacillus rhamnosus and lactobacillus plantarum to prepare a bacterial liquid; (3) pulverizing Coptidis rhizoma, Atractylodis rhizoma, fructus forsythiae and radix astragali, and sieving to obtain Chinese medicinal mixture; (4) soaking the Chinese medicinal mixture in bacteria liquid for anaerobic fermentation. The natural plant zymolyte can effectively degrade mycotoxin in the feed (the degradation rate reaches 95.0 percent), so that the enteritis of pigs is prevented and controlled; and the raw material source is rich, the preparation cost is low, the side effect is small, and the method is suitable for industrial production.
Description
Technical Field
The invention relates to the technical field of preparation of traditional Chinese medicine compositions, in particular to a natural plant glycolysis product for preventing and controlling enteritis of pigs, a preparation method and application thereof.
Background
Mycotoxin is a toxic metabolite or a secondary metabolite generated by fungi, widely exists in various food and feed raw materials, has caused many poisoning incidents at home and abroad, and brings huge economic loss to livestock and poultry breeding every year. Currently, various mycotoxins have high pollution rate and high coexistence rate, and are main challenges in the field of feed mycotoxin prevention and control. Mycotoxin enters the body through the intestinal tract, generally has strong intestinal toxicity, and is very easy to cause intestinal inflammation of pigs.
The enteritis of the pigs is a common epidemic disease of the pigs, the quality of the fed pig herds is seriously influenced, and if the enteritis is controlled improperly, the great economic loss is brought to investors. Common symptoms of porcine enteritis are: the sick pig suddenly has severe and persistent diarrhea, inappetence or anorexia, accompanied by emesis, mental retardation, lying-in preference, and initial body temperature of the sick pig is raised to 40-41 deg.C; in severe cases, the limbs are weak, the sockets collapse, the body temperature drops, and the whole body dies due to exhaustion. The enteritis of the pigs has great harm to the pig industry and extremely high death rate.
At present, the prevention and control difficulty of mycotoxin is high, the concentrated decontamination in the aspect of feed raw materials is mainly focused, and the method for degrading mycotoxin in the use of feed is less. In the aspect of preventing and treating pig enteritis, antibiotics, synthetic antibacterial drugs, biological vaccines and other modes are mainly adopted, but the treatment methods are easy to cause the pig to generate drug resistance and toxic and side effects, and the quality of pork are seriously influenced.
Therefore, the development of the natural plant zymolyte capable of effectively preventing and controlling the enteritis of the pigs has important significance for the healthy development of the pig industry.
Disclosure of Invention
In view of the above, the present invention aims to provide a natural plant fermented product for preventing and controlling enteritis of pigs, a preparation method and an application thereof, so as to solve the defects in the prior art.
In order to achieve the purpose, the invention adopts the following technical scheme:
a natural plant glycolysis product for preventing and controlling enteritis of pigs comprises the following raw materials in parts by weight: 5-10 parts of lactobacillus rhamnosus, 5-10 parts of lactobacillus plantarum, 15-25 parts of coptis chinensis, 15-20 parts of bighead atractylodes rhizome, 10-15 parts of fructus forsythiae and 25-35 parts of astragalus membranaceus;
preferably: 10 parts of lactobacillus rhamnosus, 5 parts of lactobacillus plantarum, 20 parts of coptis chinensis, 15 parts of bighead atractylodes rhizome, 15 parts of fructus forsythiae and 35 parts of astragalus membranaceus.
In the invention:
lactobacillus rhamnosus (LGG) mostly exists in intestinal tracts of human beings and animals, and the taxonomy of bacteria belongs to Lactobacillus and rhamnosus subspecies, is anaerobic acid-resistant and spore-free gram-positive probiotic bacteria, and has the biological characteristics of acid resistance, bile salt resistance, multiple antibiotics resistance and the like. After the LGG is colonized and propagated in the intestinal environment of a host, only intestinal epithelial cells attached to the host can become a layer of biological barrier of intestinal mucosa, so that the intestinal mucosal barrier capability of the host is improved; moreover, the LGG can be used for regulating the structure and the function of the microecological flora in the intestinal tract of the host, so that the microecological flora reaches a balanced state, and the aim of improving the function of the digestive tract system of the host is fulfilled.
The lactobacillus plantarum is one of lactic acid bacteria, and has optimal growth temperature of 30-35 deg.C, and anaerobic or facultative anaerobic property. The health care effect is as follows: firstly, has a certain immunoregulation function; ② the medicine has inhibiting effect on pathogenic bacteria; ③ reducing the content of serum cholesterol and preventing cardiovascular diseases; fourthly, maintaining the balance of the flora in the intestinal tract; promoting the absorption of nutrient substances; sixthly, relieving lactose intolerance; inhibit the formation of tumor cells, etc.
The Coptidis rhizoma is dried rhizome of Coptidis rhizoma, Coptidis rhizoma or Coptis Teeta wall of Ranunculaceae. Has the effects of clearing heat, eliminating dampness, purging fire and removing toxicity. Mainly treats damp-heat fullness, vomiting and acid regurgitation, diarrhea, jaundice, high fever and coma, hyperactivity of heart-fire, vexation and insomnia, palpitation and restlessness, blood heat and hematemesis and epistaxis, conjunctival congestion, toothache, diabetes, carbuncle and swelling and sore; it is used externally to treat eczema, eczema and purulent ear canal.
The bighead atractylodes rhizome is a common and important bulk Chinese medicinal material and has the functions of tonifying spleen and strengthening stomach, eliminating dampness and promoting diuresis, stopping sweating and preventing miscarriage and the like. The rhizome of Atractylodes macrocephala contains volatile oil, the main components of the oil are atractylone, atractylol, atractylenolide, etc., and the drug has certain curative effect on ascites due to cirrhosis, primary liver cancer, Meniere's syndrome, chronic lumbago, acute enteritis, leukopenia, etc.
Lian Qiao clears away heat and toxic material, dissipates nodulation and reduces swelling. It is indicated for warm heat, erysipelas, macula, superficial infection, abscess, swelling, scrofula and stranguria.
Radix astragali is root of Astragalus membranaceus bge of Leguminosae. The main functional indications are as follows: tonify qi, strengthen superficies, expel toxin, expel pus, induce diuresis, and promote granulation. Can be used for treating deficiency of vital energy, asthenia, chronic diarrhea, proctoptosis, spontaneous perspiration, edema, uterine prolapse, albuminuria due to chronic nephritis, diabetes, and unhealed wound.
A preparation method of natural plant glycolysis product for preventing and controlling pig enteritis specifically comprises the following steps:
(1) weighing the raw materials according to the parts by weight of the natural plant glycolysis product for preventing and controlling the enteritis of the pigs;
(2) mixing lactobacillus rhamnosus and lactobacillus plantarum to prepare a bacterial liquid for later use;
(3) pulverizing Coptidis rhizoma, Atractylodis rhizoma, fructus forsythiae and radix astragali, and sieving to obtain Chinese medicinal mixture;
(4) soaking the traditional Chinese medicine mixture in a bacterial liquid for anaerobic fermentation to obtain the natural plant zymolyte for preventing and controlling the enteritis of the pigs.
Further, in the step (4), the temperature of the bacterial liquid is 35 ℃.
The invention also discloses application of the natural plant zymolyte for preventing and controlling pig enteritis or the natural plant zymolyte prepared by the preparation method in feed processing.
According to the technical scheme, compared with the prior art, the invention has the following beneficial effects:
the natural plant zymolyte can effectively degrade mycotoxin in the feed (the degradation rate reaches 95.0 percent), so that the enteritis of pigs is prevented and controlled; and the raw material source is rich, the preparation cost is low, the side effect is small, and the method is suitable for industrial production.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The natural plant glycolysis product for preventing and controlling pig enteritis comprises the following raw materials by weight: 10g of lactobacillus rhamnosus, 5g of lactobacillus plantarum, 20g of coptis chinensis, 15g of bighead atractylodes rhizome, 15g of fructus forsythiae and 35g of astragalus membranaceus;
the preparation method of the natural plant glycolysis product for preventing and controlling the enteritis of the pigs specifically comprises the following steps:
(1) weighing the raw materials according to the weight of the natural plant glycolysis product for preventing and controlling the enteritis of the pigs;
(2) mixing lactobacillus rhamnosus and lactobacillus plantarum to prepare a bacterial liquid for later use;
(3) pulverizing Coptidis rhizoma, Atractylodis rhizoma, fructus forsythiae and radix astragali, and sieving to obtain Chinese medicinal mixture;
(4) soaking the traditional Chinese medicine mixture in a bacterial liquid at 35 ℃ for anaerobic fermentation to obtain the natural plant zymolyte for preventing and controlling the enteritis of the pigs.
Example 2
The natural plant glycolysis product for preventing and controlling pig enteritis comprises the following raw materials by weight: 5g of lactobacillus rhamnosus, 5g of lactobacillus plantarum, 15g of coptis chinensis, 15g of bighead atractylodes rhizome, 10g of fructus forsythiae and 25g of astragalus membranaceus;
the preparation method of the natural plant glycolysis product for preventing and controlling the enteritis of the pigs specifically comprises the following steps:
(1) weighing the raw materials according to the weight of the natural plant glycolysis product for preventing and controlling the enteritis of the pigs;
(2) mixing lactobacillus rhamnosus and lactobacillus plantarum to prepare a bacterial liquid for later use;
(3) pulverizing Coptidis rhizoma, Atractylodis rhizoma, fructus forsythiae and radix astragali, and sieving to obtain Chinese medicinal mixture;
(4) and soaking the traditional Chinese medicine mixture in a bacterial liquid at 35 ℃ for anaerobic fermentation to obtain the natural plant zymolyte for preventing and controlling the enteritis of the pigs.
Example 3
The natural plant glycolysis product for preventing and controlling pig enteritis comprises the following raw materials by weight: 10g of lactobacillus rhamnosus, 10g of lactobacillus plantarum, 25g of coptis chinensis, 20g of bighead atractylodes rhizome, 15g of fructus forsythiae and 35g of astragalus membranaceus;
the preparation method of the natural plant glycolysis product for preventing and controlling the enteritis of the pigs specifically comprises the following steps:
(1) weighing the raw materials according to the weight of the natural plant glycolysis product for preventing and controlling the enteritis of the pigs;
(2) mixing lactobacillus rhamnosus and lactobacillus plantarum to prepare a bacterial liquid for later use;
(3) pulverizing Coptidis rhizoma, Atractylodis rhizoma, fructus forsythiae and radix astragali, and sieving to obtain Chinese medicinal mixture;
(4) soaking the traditional Chinese medicine mixture in a bacterial liquid at 35 ℃ for anaerobic fermentation to obtain the natural plant zymolyte for preventing and controlling the enteritis of the pigs.
Performance testing
600 fattening pigs are selected and randomly divided into 3 groups of 200 pigs each, and the groups are respectively used as a test group 1, a test group 2 and a control group. 2 kg/ton of the natural plant zymolyte prepared in the example 1 and the example 2 is added into the daily feed of the test group 1 and the test group 2 respectively; the control group was treated with the drug, mixed and fed for 30 days.
1. PCR detection
Rectal swabs are selected respectively before and after the test period to collect the test pig manure for PCR detection. If the detection result is positive, the pig is judged to be suffered from enteritis. And recording the number of positive pigs and counting, wherein the positive rate is the number of positive pigs/the total number of test pigs, and the reduction rate is (positive rate before test-positive rate after test)/positive rate before test. The results are shown in Table 1.
TABLE 1 comparison of PCR detection results before and after the test
| Test group | Positive rate before test (%) | Positive rate after test (%) | Reduction ratio (%) |
| Test group 1 | 49 | 2 | 95.92 |
| Test group 2 | 42 | 3 | 92.85 |
| Control group | 38 | 15 | 60.53 |
From the above test data, it is understood that the PCR test positive rate of the test pigs was significantly decreased after the natural plant yeast extracts prepared in examples 1 and 2 were continuously administered for 30 days. Wherein, the test group 1 is up to 95.92 percent, and then the test group 2, and the difference among the groups is that the raw materials of the groups have different dosages; the control group was a drug-directed treatment, but the therapeutic effect was not significant as compared with test group 1 and test group 2.
2. Growth performance
Before and after the test period, weighing and recording the test pigs, and if the test pigs die or are eliminated, weighing and recording the test pigs, and performing a autopsy on the dead pigs to determine the death reason. At the end of the test, the recorded data were analyzed and the daily and total weight gains of the test and control groups were compared. The results are shown in Table 2.
TABLE 2 comparison of growth Performance before and after testing
| Test group | Daily gain (g) | Total weight gain (kg) |
| Test group 1 | 796±25 | 31.18±1.4 |
| Test group 2 | 765±16 | 29.32±2.3 |
| Control group | 722±21 | 27.43±1.5 |
As can be seen from Table 2, the daily gain and total gain effects of test group 1 and test group 2 are significantly better than those of the control group, with test group 1 being the most effective.
The tests prove that the enteritis of the pigs can influence the gastrointestinal absorption of the pigs, thereby influencing the growth performance of the pigs. The invention can treat pig enteritis and ensure the good growth performance of test pigs.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (5)
1. The natural plant glycolysis product for preventing and controlling enteritis of pigs is characterized by comprising the following raw materials in parts by weight: 5-10 parts of lactobacillus rhamnosus, 5-10 parts of lactobacillus plantarum, 15-25 parts of coptis chinensis, 15-20 parts of bighead atractylodes rhizome, 10-15 parts of fructus forsythiae and 25-35 parts of astragalus membranaceus.
2. The natural plant zymolyte for preventing and controlling enteritis of pigs according to claim 1, which comprises the following raw materials in parts by weight: 10 parts of lactobacillus rhamnosus, 5 parts of lactobacillus plantarum, 20 parts of coptis chinensis, 15 parts of bighead atractylodes rhizome, 15 parts of fructus forsythiae and 35 parts of astragalus membranaceus.
3. A preparation method of natural plant zymolyte for preventing and controlling enteritis of pigs is characterized by comprising the following steps:
(1) weighing the raw materials according to the parts by weight of the natural plant glycolysis product for preventing and controlling the enteritis of the pigs, which is described in the claim 1 or 2;
(2) mixing lactobacillus rhamnosus and lactobacillus plantarum to prepare a bacterial liquid for later use;
(3) pulverizing Coptidis rhizoma, Atractylodis rhizoma, fructus forsythiae and radix astragali, and sieving to obtain Chinese medicinal mixture;
(4) soaking the traditional Chinese medicine mixture in a bacterial liquid for anaerobic fermentation to obtain the natural plant zymolyte for preventing and controlling the enteritis of the pigs.
4. The method for preparing natural plant zymolyte for preventing and controlling enteritis of pigs according to claim 3, wherein in the step (4), the temperature of the bacterial liquid is 35 ℃.
5. Use of the natural plant zymolyte for preventing and controlling porcine enteritis according to claims 1-2 or the natural plant zymolyte for preventing and controlling porcine enteritis prepared by the preparation method according to claims 3-4 in feed processing.
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