CN108813124A - A kind of feed and preparation method thereof improving Eriocheir sinensis anti-stress ability - Google Patents

A kind of feed and preparation method thereof improving Eriocheir sinensis anti-stress ability Download PDF

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CN108813124A
CN108813124A CN201810553466.2A CN201810553466A CN108813124A CN 108813124 A CN108813124 A CN 108813124A CN 201810553466 A CN201810553466 A CN 201810553466A CN 108813124 A CN108813124 A CN 108813124A
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mixture
feed
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stress ability
vitamin
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谢真
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Shenzhen Lingdu Intelligent Control Technology Co Ltd
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    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/22—Animal feeding-stuffs from material of animal origin from fish
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/105—Aliphatic or alicyclic compounds
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/174—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/26—Compounds containing phosphorus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/30—Oligoelements
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish
    • Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

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Abstract

The invention discloses a kind of feeds and preparation method thereof for improving Eriocheir sinensis anti-stress ability, and wheat bran, dregs of beans, rapeseed dregs and traditional Chinese medicinal components are mixed to get the first mixture, fermentation substrate is made after water is added；Probiotics is added in fermentation substrate, is fermented to obtain fermentation material；Supernatant liquor and lower sediment are obtained after being centrifuged to fermentation material, supernatant liquor are freeze-dried to obtain freeze-drying object, and lower sediment is dried to obtain the second mixture；After multi-vitamins, phagostimulant are mixed with freeze-drying object, microcapsule granule is made using it as core material；The 4th mixture is obtained after corn flour, wheat-middlings etc. are mixed, and is carried out extruding and is pelletized to obtain swelling granular；It is mixed with microcapsule granule by preset ratio after swelling granular is dried, is improved the feed of Eriocheir sinensis anti-stress ability.The stress ability of Eriocheir sinensis can be improved by the way that feed made from traditional Chinese medicinal components and probiotics fermention and extruding granulation technique is added in the present invention.

Description

A kind of feed and preparation method thereof improving Eriocheir sinensis anti-stress ability
Technical field
The present invention relates to field of feed more particularly to it is a kind of improve Eriocheir sinensis anti-stress ability feed and its Preparation method.
Background technique
Eriocheir japonica sinensis is commonly called as river crab, steamed crab, is the Important Economic breed variety of China's fresh water fishery, because taste is fresh It is beauty, fine and tender taste, full of nutrition and very popular.In the breeding process of Eriocheir sinensis, due to weather, water temperature, The influence of the factors such as water quality, it is easy to cause it that stress reaction occurs, thus will affect the normal growth and development of Eriocheir sinensis, Large Scale Death is even resulted in, huge economic loss can be caused to raiser, therefore, it is anti-to develop a kind of raising Eriocheir sinensis The feed of stress ability has great importance.
Summary of the invention
The main purpose of the present invention is to provide a kind of feed for improving Eriocheir sinensis anti-stress ability and its preparation sides Method, it is intended to a kind of feed that Eriocheir sinensis anti-stress ability can be improved is researched and developed, to improve the anti-stress ability of Eriocheir sinensis.
To achieve the above object, the present invention provides a kind of feed for improving Eriocheir sinensis anti-stress ability, with weight hundred Divide than indicating, the group of the feed for improving Eriocheir sinensis anti-stress ability becomes：Corn flour 18%-28%, wheat-middlings 12%- 20%, wheat bran 5%-13%, fish meal 8%-14%, dregs of beans 10%-20%, rapeseed dregs 2%-6%, meat meal tankage 2%-4%, fish oil 1%-3%, choline chloride 0.2%-0.6%, salt 0.1%-0.4%, traditional Chinese medicinal components 6%-14%, probiotics 0.5%-2%, Shelling element 0.5%-2%, multi-vitamins 0.1%-0.5%, composite mineral matter 1%-3%, phagostimulant 0.1%-0.2% and anti- Mould dose of 0.05%-0.1%.
Optionally, the composition and weight percent of the traditional Chinese medicinal components are as follows：Cortex albiziae 10%-20%, Radix Codonopsis 9%- 14%, root bark of tree peony 8%-15%, Herba Andrographitis 5%-12%, radix scutellariae 11%-17%, Radix Angelicae Sinensis 10%-16%, wilsonii 10%-15%, Folium isatidis 6%-12%, campanulaceae 5%-9% and dried orange peel 4%-7%.
Optionally, the probiotics includes bacillus licheniformis, bacillus subtilis, lactic acid bacteria, Bifidobacterium and butyric acid At least two of clostridium.
Optionally, the composition of the multi-vitamins and weight percent are as follows：Retinol1 5%-22%, vitamin B10.5%-4.5%, vitamin B23%-8%, vitamin B52%-5%, vitamin B63%-6%, vitamin C 7%- 13%, vitamin D315%-23%, vitamin E 3%-7%, vitamin K320%-28%, folic acid 2%-8%, niacin 5%- 12% and biotin 0.3%-0.7%.
Optionally, the composition of the composite mineral matter and weight percent are as follows：Copper sulphate 7%-17%, ferrous sulfate 6%-12%, zinc lactate 8%-15%, magnesium sulfate 3%-8%, sodium selenite 0.04%-0.1%, potassium iodide 0.3%-0.7%, Manganese sulfate 4%-9% and calcium dihydrogen phosphate 45%-55%.
In addition, to achieve the above object, the present invention also provides a kind of feeds for improving Eriocheir sinensis anti-stress ability Preparation method, the preparation method comprises the following steps：
S1：It is mixed after wheat bran, dregs of beans, rapeseed dregs and traditional Chinese medicinal components are weighed in proportion, obtains the first mixture, root Fermentation substrate is made according to the water that 2 times of weight are added in the weight of first mixture；
S2：It is added to after probiotics is weighed in proportion in the fermentation substrate, ferments after mixing, sent out Ferment object, wherein fermentation temperature is 35 DEG C -55 DEG C, and fermentation time is -120h for 24 hours；
S3：Supernatant liquor and lower sediment are obtained after being centrifuged to the fermentation material, the supernatant liquor is carried out cold It is lyophilized dry, obtains corresponding freeze-drying object, and dry with 30 DEG C -40 DEG C of temperature to the lower sediment, obtain Two mixtures；
S4：It is mixed after multi-vitamins and phagostimulant are weighed in proportion with the freeze-drying object, obtains third Mixture, and microcapsule granule is made using the third mixture as core material；
S5：By corn flour, wheat-middlings, fish meal, meat meal tankage, fish oil, choline chloride, salt, shelling element, composite mineral matter and prevent Mould dose in proportion weigh after mixed, after pulverization process cross 80 meshes, obtain the 4th mixture；
S6：4th mixture is put into quality-adjusting device, is passed through that vapor is quenched, and will be conditioned after the 4th mixing Object enters extrusion granulator and pelletizes, and obtains swelling granular；
S7：It after swelling granular drying, is mixed with the microcapsule granule by preset ratio, obtains described mention The feed of high Eriocheir sinensis anti-stress ability.
Optionally, further comprising the steps of before step S1：
S8：By cortex albiziae, Radix Codonopsis, the root bark of tree peony, Herba Andrographitis, radix scutellariae, Radix Angelicae Sinensis, wilsonii, folium isatidis, campanulaceae and dried orange peel respectively into The dry pretreatment of row, wherein pretreatment temperature is 30 DEG C -50 DEG C, pretreatment time 2h-3h；
S9：Taking weight percent content is respectively 10%-20% cortex albiziae, 9%-14% Radix Codonopsis, the 8%-15% root bark of tree peony, 5%-12% Herba Andrographitis, 11%-17% radix scutellariae, 10%-16% Radix Angelicae Sinensis, 10%-15% wilsonii, 6%-12% folium isatidis, 5%-9% campanulaceae and 4%-7% dried orange peel, are crushed, and are uniformly mixed after crossing 60 meshes, the traditional Chinese medicinal components are made.
Optionally, step S4 includes：
S41：It is mixed after multi-vitamins and phagostimulant are weighed in proportion with the freeze-drying object, obtains third Mixture；
S42：Using the third mixture as core material, according to the weight of the third mixture by core wall than 1:3 weigh pair The wall material of weight is answered, wherein it with weight ratio is 1 that the wall material, which is gelatin and trehalose,:2-1:5 are mixed to get；
S43：Wall material described in deionized water dissolving is added, so that the concentration of wall material solution is 20%-35%；
S44：Emulsifier and the third mixture are added in the wall material solution, it is after mixing evenly, equal using high pressure Matter machine carries out homogeneous, obtains homogeneous latex emulsion, wherein homogenization pressure 20MPa-50MPa, mean time 3min-7min；
S45：The homogeneous latex emulsion is spray-dried, microcapsule granule is obtained, wherein the condition of spray drying is： Inlet air temperature is 150 DEG C -170 DEG C, and leaving air temp is 60 DEG C -80 DEG C, feed rate 6mL/min-10mL/min.
Optionally, step S8 includes：
S81：4th mixture is put into quality-adjusting device, is passed through that temperature is 90 DEG C -100 DEG C, quality is the described 4th The quenched 5min-9min of the vapor of the 8%-15% of mixture gross mass；
S82：The 4th mixture after will be conditioned 90 DEG C -100 DEG C at a temperature of enter extrusion granulator and carry out Granulation, obtains swelling granular.
Optionally, in step s 9, the drying temperature is 95 DEG C -105 DEG C, and the preset ratio is 7:1-9:1.
The present invention provides a kind of feed and preparation method thereof for improving Eriocheir sinensis anti-stress ability, the composition of the feed For：It is corn flour, wheat-middlings, wheat bran, fish meal, dregs of beans, rapeseed dregs, meat meal tankage, fish oil, choline chloride, salt, traditional Chinese medicinal components, prebiotic Bacterium, shelling element, multi-vitamins, composite mineral matter, phagostimulant and mould inhibitor.Wheat bran, dregs of beans, rapeseed dregs and traditional Chinese medicinal components are pressed It is mixed after ratio weighing, obtains the first mixture, fermentation substrate is made in the water that corresponding weight is added；In proportion by probiotics It is added in fermentation substrate after weighing, ferments after mixing, obtain fermentation material；On being obtained after being centrifuged to fermentation material Layer clear liquid and lower sediment, are freeze-dried supernatant liquor, obtain corresponding freeze-drying object, and carry out to lower sediment Drying, obtains the second mixture；It mixes, obtains with freeze-drying object after multi-vitamins and phagostimulant are weighed in proportion Third mixture, and microcapsule granule is made using third mixture as core material；By corn flour, wheat-middlings, fish meal, meat meal tankage, fish oil, Choline chloride, salt, shelling element, composite mineral matter and mould inhibitor are mixed after weighing in proportion, and 80 are crossed after pulverization process Mesh obtains the 4th mixture；4th mixture is put into quality-adjusting device, is passed through that vapor is quenched, and will be conditioned after Four mixtures enter extrusion granulator and pelletize, and obtain swelling granular；After swelling granular is dried, with microcapsule granule It is mixed by preset ratio, is improved the feed of Eriocheir sinensis anti-stress ability.By the above-mentioned means, can reach following Beneficial effect：
1) traditional Chinese medicinal components with raising anti-stress ability are added in feed, can be improved by the feeding feed The anti-stress ability of magnificent Eriocheir, the traditional Chinese medicinal components can also enhance combined immune power of Eriocheir sinensis etc., can effectively reduce The death rate can further improve herbal medicine efficacy, and can in addition, ferment to traditional Chinese medicinal components by probiotics fermention technology It eliminates Chinese medicine toxic side effect and changes herbal nature；
2) it is fermented by probiotics to wheat bran, dregs of beans, rapeseed dregs, can effectively reduce dregs of beans, the nocuousness in rapeseed dregs Substance improves the safety of feed, meanwhile, after probiotics fermention, the substance that a part is difficult to digest and assimilate can be become Simple substance easy to digest and absorb, improves the nutritive value of feed, promotes the digestion and absorption of Eriocheir sinensis, accelerate its life Long speed can also convert the small-molecule substances such as monosaccharide and oligosaccharides for the macromolecular substances that cellulose, pectic substance etc. are difficult to degrade, And generate a variety of organic acids, micro-element, biological enzyme and a variety of growth factors, greatly improve institute's fermentation materials trophic level and Digestive utilization ratio reduces feeding cost to improve efficiency of feed utilization, improves breeding income；
3) by prebiotic fermentation technique and microcapsules technology, so that contain a large amount of beneficial microbe viable bacteria in feed, it can The intestinal microecology balance for establishing Eriocheir sinensis, adjusts its stomach balance, to promote nutrient absorption, improves feed conversion Rate, meanwhile, probiotics and some active materials are made by microcapsule granule by microcapsules technology, high temperature is can avoid and kills part The benefit materials such as probiotics and destruction protein active agent；
4) it is added in the growth and development process of Eriocheir sinensis and maintains dimension life necessary to eubolism and physiological function Element and minerals, can be improved the nutrition full price and utilization rate of feed, enhance the anti-stress ability of Eriocheir sinensis；
5) raw material sources required for the present invention are extensive, safe and non-toxic, cheap, are suitble to large-scale popularization, and fermented Feed afterwards, the holding time is longer, has the stronger market competitiveness.
Detailed description of the invention
Fig. 1 is a flow diagram of the preparation method for the feed that the present invention improves Eriocheir sinensis anti-stress ability；
Fig. 2 is the another flow diagram of the preparation method for the feed that the present invention improves Eriocheir sinensis anti-stress ability.
The embodiments will be further described with reference to the accompanying drawings for the realization, the function and the advantages of the object of the present invention.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete Whole description, it is clear that described embodiment is only a part of the embodiments of the present invention, instead of all the embodiments.Base Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts it is all its His embodiment, shall fall within the protection scope of the present invention.
In addition, being somebody's turn to do " first ", " second " etc. if relating to the description of " first ", " second " etc. in the embodiment of the present invention Description be used for description purposes only, be not understood to indicate or imply its relative importance or implicitly indicate indicated skill The quantity of art feature." first " is defined as a result, at least one spy can be expressed or be implicitly included to the feature of " second " Sign.
It in addition, the technical solution between each embodiment can be combined with each other, but must be with ordinary skill Based on personnel can be realized, this technical side will be understood that when the combination of technical solution appearance is conflicting or cannot achieve The combination of case is not present, also not within the claimed scope of the invention.
The present invention provides a kind of feed for improving Eriocheir sinensis anti-stress ability, the raising Eriocheir sinensis resisting stress The group of the feed of ability becomes：Corn flour, wheat-middlings, wheat bran, fish meal, dregs of beans, rapeseed dregs, meat meal tankage, fish oil, choline chloride, food Salt, traditional Chinese medicinal components, probiotics, shelling element, multi-vitamins, composite mineral matter, phagostimulant and mould inhibitor.
Wherein, the composition of traditional Chinese medicinal components includes：Cortex albiziae, Radix Codonopsis, the root bark of tree peony, Herba Andrographitis, radix scutellariae, Radix Angelicae Sinensis, wilsonii, smalt Leaf, campanulaceae and dried orange peel.Wherein, cortex albiziae has the function of nourishing heart blood, tranquilizing mind, for caused by heart-liver blood deficiency, mind dystrophy The not peaceful chronic diseases marked by deficiency of vital energy of minds such as restlessness of asrhenia type and insomnia, palpitaition secondary, amnesia seized with terror.Radix Codonopsis bowl spares, QI invigorating are promoted the production of body fluid, and weakness of the spleen and the stomach, qi and blood two is controlled Thanks to, the tired inability of body, deficiency of food is thirsty, chronic diarrhea, rectal prolapse, have it is calm it is antipyretic, drop blood, promote hematopoiesis function, be antifatigue, antitumor The effects of effect and blood-nourishing tonifying Qi.The root bark of tree peony has the effect of clearing heat and cooling blood, activating microcirculation and removing stasis medicinal, has anti-inflammatory, antibacterial, anticoagulant, promoting blood circulation to lead to The effects of network, enhancing holddown, T lymphocyte number can be improved, and enhance its function, to improve body immunity.Punching Lotus has effects that clearing heat and detoxicating, anti-inflammatory, swelling and pain relieving, moreover it is possible to enhance the activity of lysozyme, enhance immune function.Scutellaria has clearly Hot eliminating dampness, purging fire for removing toxin, hemostasis, miscarriage prevention and other effects cure mainly warm heat disease, the infection of the upper respiratory tract, cough with lung heat, neonatal jaundice caused by dampness-heat, lung The diseases such as inflammation, dysentery, hemoptysis, hot eyes, threatened abortion, hypertension, carbuncle swells furuncle sore.Radix Angelicae Sinensis, which has, enriches blood, invigorates blood circulation, menstruction regulating and pain relieving, profit The effect of dry laxation, the main all cards of the deficiency of blood；Irregular menstruation；Menostasis；Dysmenorrhea；Abdominal mass knot is poly-；Metrorrhagia and metrostaxis；Asthenia cold abdominalgia；Flaccidity syndrome and arthralgia syndrome；Skin fiber crops Wood；Intestines are dry and shit is difficult；Weight after dysentery with bloody stool；Ulcer sores；Injury from falling down；The stasis of blood that can reduce red blood cell in recycling is stagnant and collect, and makes micro- blood Pipe permeability reduces, and oozes out and reduces around capilary.Wilsonii has improvement heart and brain blood circulation, adjusts nervous centralis and interior point Secrete the function of system, moreover it is possible to which the phagocytic function for promoting reticuloendothelial system enhances its ability for swallowing foreign matter and antigen, and improves The concentration of Serum Antibody.Folium isatidis has the effect of clearing heat and detoxicating, blood cooling and ecchymoses removing, is used for pyreticosis hyperpyrexia polydipsia；Coma；Spot Rash；It spits blood；Bleeding from five sense organs or subcutaneous tissue；Jaundice；Dysentery；Erysipelas；Sore throat；Aphtha；Mumps.Campanulaceae has the effect of promoting the dispersing function of the lung, relieving sore-throat, eliminating the phlegm, apocenosis, For coughing with a lot of sputum, chest distress, pharyngalgia hoarse voice, lung carbuncle pyemesis.Dried orange peel has regulating qi-flowing for strengthening spleen, and eliminating dampness and eliminating phlegm effect is used for Abdominal fullness and distention, deficiency of food are vomited and diarrhoea, and coughing with a lot of sputum is equal with hawthorn and uses, it may also be used for stagnation of QI due to dyspepsia and abdominal distention.
Probiotics may include in bacillus subtilis, bacillus licheniformis, lactic acid bacteria, Bifidobacterium and clostridium butyricum At least two.Wherein, bacillus subtilis can produce subtilin, polymyxins, nystatin, short during thalli growth Bacitracin isoreactivity substance, these active materials have apparent inhibition to make the conditioned pathogen of pathogenic bacteria or autogenous infection With；The free oxygen in enteron aisle can be also consumed rapidly, causes enteron aisle hypoxemia, promoted to grow beneficial to anaerobic bacteria, inhibited other causes indirectly Pathogen growth；In addition, the growth and development of animal immune organ can be stimulated, T, bone-marrow-derived lymphocyte are activated, improve immunoglobulin and is resisted Body is horizontal, enhances cellular immunity and humoral immune function, improves herd immunity.Bacillus licheniformis can promote normal in enteron aisle The growth of physiological anaerobic bacteria adjusts intestinal bacilli illness, restores function of intestinal canal；Also there is special efficacy to enterobacterial infection, it is right Light-duty or heavy chordapsus, light-duty and medium-sized acute bacillary dysentery etc., there is obvious curative effects；Moreover it is possible to generate resistant activity Substance, and there is unique biology take oxygen mechanism, the growth and breeding of pathogenic bacteria can be inhibited.Lactic acid bacteria can increase intestinal beneficial Flora improves gastrointestinal function, restores intestinal flora balance, forms antibacterial biological barrier, maintaining healthy；It can also inhibit corruption The breeding of bacterium, the toxin that resolution spoilage organisms generates, removes enteron aisle rubbish；In addition, also having immunoregulation effect, enhance animal body Immunity and resistance.Bifidobacterium is a kind of important intestinal beneficial microbes, has biological barrier, battalion to human health The effect of supporting, immunological enhancement, improves a variety of important physiological functions such as gastrointestinal function, anti-aging at antitumor action.Butyric acid Clostridium can adjust intestinal colony balance, promote intestine beneficial bacteria colony proliferation, and generate prebiotic product in enteron aisle；Also there is enhancing to exempt from It is related can also to treat acute and chronic diarrhea, intestinal irritable syndrome, antibiotic because of caused by flora imbalance for epidemic disease, growth promotion function The symptoms such as property enteritis, constipation.
Multi-vitamins include vitamin A, vitamin B1, vitamin B2, vitamin B5, vitamin B6, vitamin C, dimension life Plain D3, vitamin E, vitamin K3, folic acid, niacin and biotin, maintained in growth and development process for supplementing Eriocheir sinensis The nutrition full price and utilization rate of feed can be improved in the addition of vitamin necessary to eubolism and physiological function, vitamin, Enhance the anti-stress ability of Eriocheir sinensis.
Composite mineral matter include copper sulphate, ferrous sulfate, zinc lactate, magnesium sulfate, sodium selenite, potassium iodide, manganese sulfate and Calcium dihydrogen phosphate, for supplementing copper, iron needed for the growth and development process of Eriocheir sinensis, zinc, magnesium, selenium, iodine, potassium, manganese, calcium With the minerals such as phosphorus.
The present invention also provides a kind of preparation methods of the preparation method of feed for improving Eriocheir sinensis anti-stress ability.For Facilitate explanation, illustrates each group at the additional proportion of ingredient by taking feed relative 100g as an example in each of the embodiments described below.
Embodiment one,
Firstly, cortex albiziae, Radix Codonopsis, the root bark of tree peony, Herba Andrographitis, radix scutellariae, Radix Angelicae Sinensis, wilsonii, folium isatidis, campanulaceae and dried orange peel are distinguished Pretreatment is dried, pretreatment temperature is 50 DEG C, pretreatment time 2h.Then it weighs respectively pretreated each through drying Chinese herbal medicine, specifically, weighing 10g cortex albiziae, 9g Radix Codonopsis, the 8g root bark of tree peony, 12g Herba Andrographitis, 15g radix scutellariae, 16g Radix Angelicae Sinensis, 11g thorn five Add, 6g folium isatidis, 9g campanulaceae and 4g dried orange peel, crushed, is uniformly mixed after crossing 60 meshes, traditional Chinese medicinal components are made.
It is mixed, is obtained secondly, weighing 13g wheat bran, 20g dregs of beans, 6g rapeseed dregs and the above-mentioned mixed traditional Chinese medicinal components of 6g Fermentation substrate is made to the first mixture, and according to the water that 2 times of weight are added in the weight of first mixture, then weighs 0.5g benefit Raw bacterium is added in the fermentation substrate, is uniformly mixed and is placed in fermentation vat, ferments, obtain fermentation material.Wherein, this is prebiotic Bacterium is that bacillus subtilis and lactic acid bacteria press 2:What 1 ratio was mixed to get, fermentation temperature is 35 DEG C, fermentation time 56h.? To after fermentation material, supernatant liquor and lower sediment are obtained after being centrifuged to the fermentation material, it is dry to carry out freezing to supernatant liquor It is dry, corresponding freeze-drying object is obtained, and dry with 30 DEG C of temperature to the lower sediment, obtains the second mixture.
Again, 0.1g multi-vitamins are weighed and 0.2g phagostimulant is added in the freeze-drying object, is uniformly mixed, obtains Third mixture, and microcapsule granule is made using the third mixture as core material.It wherein, is to be tieed up to give birth to by 15g in multi-vitamins Plain A, 2.3g vitamin B1, 8g vitamin B2, 4g vitamin B5, 3g vitamin B6, 7g vitamin C, 23g vitamin D3, 3g dimension life Plain E, 20g vitamin K3, 2g folic acid, 12g niacin and 0.7g biotin obtain after evenly mixing (wherein, multi-vitamins are pressed Illustrate the additional proportion of each vitamin constituent for 100g).Specifically, microcapsule granule the production method is as follows：Claim It takes 0.1g multi-vitamins and 0.2g phagostimulant to be added in the freeze-drying object, and is mixed, obtain third mixture；With The third mixture is core material, according to the weight of the third mixture by core wall than 1:3 weigh the wall material of corresponding weight, wherein should It with weight ratio is 1 that wall material, which is gelatin and trehalose,:5 are mixed to get；The deionized water dissolving wall material is added, so that wall material solution Concentration be 35%；Emulsifier and the third mixture are added in the wall material solution, after mixing evenly, use is high-pressure homogeneous Machine carries out homogeneous, obtains homogeneous latex emulsion, wherein homogenization pressure 20MPa, mean time 7min；The homogeneous latex emulsion is sprayed Mist is dry, obtains microcapsule granule, wherein the condition of spray drying is：Inlet air temperature is 160 DEG C, and leaving air temp is 80 DEG C, into Stream amount is 8mL/min.
Then, weigh respectively 18g corn flour, 20g wheat-middlings, 8g fish meal, 2g meat meal tankage, 2g fish oil, 0.6g choline chloride, 0.4g salt, 2.0g shelling element, 1.0g composite mineral matter and 0.1g mould inhibitor are mixed, and 80 meshes are crossed after pulverization process, Obtain the 4th mixture.Wherein, composite mineral matter be by 17g copper sulphate, 8g ferrous sulfate, 15g zinc lactate, 8g magnesium sulfate, (wherein, the grandidierite that 0.1g sodium selenite, 0.3g potassium iodide, 6.6g manganese sulfate and 45g calcium dihydrogen phosphate obtain after evenly mixing Substance is by the additional proportion for illustrating each mineral matter constituent for 100g).Then, the 4th mixture is put into quality-adjusting device In, be passed through that vapor is quenched, and will be conditioned after the 4th mixture enter extrusion granulator and pelletize, obtain extruding Particle.Specifically, the preparation process of swelling granular is：4th mixture is put into quality-adjusting device, being passed through temperature is 95 DEG C, matter Amount be the 4th mixture gross mass the quenched 9min of 8% vapor, then will be conditioned after the 4th mixture at 100 DEG C At a temperature of enter extrusion granulator pelletize, obtain swelling granular.
Finally, pressing 9 with microcapsule granule after swelling granular is dried with 95 DEG C of drying temperature:1 ratio carries out Mixing, is improved the feed of Eriocheir sinensis anti-stress ability.
Embodiment two,
Firstly, cortex albiziae, Radix Codonopsis, the root bark of tree peony, Herba Andrographitis, radix scutellariae, Radix Angelicae Sinensis, wilsonii, folium isatidis, campanulaceae and dried orange peel are distinguished Pretreatment is dried, pretreatment temperature is 40 DEG C, pretreatment time 3h.Then it weighs respectively pretreated each through drying Chinese herbal medicine, specifically, weighing 18g cortex albiziae, 9g Radix Codonopsis, the 13g root bark of tree peony, 7g Herba Andrographitis, 13g radix scutellariae, 12g Radix Angelicae Sinensis, 10g thorn five Add, 8g folium isatidis, 5g campanulaceae and 5g dried orange peel, crushed, is uniformly mixed after crossing 60 meshes, traditional Chinese medicinal components are made.
It is mixed, is obtained secondly, weighing 9g wheat bran, 15g dregs of beans, 3g rapeseed dregs and the above-mentioned mixed traditional Chinese medicinal components of 10g Fermentation substrate is made to the first mixture, and according to the water that 2 times of weight are added in the weight of first mixture, then weighs 0.5g benefit Raw bacterium is added in the fermentation substrate, is uniformly mixed and is placed in fermentation vat, ferments, obtain fermentation material.Wherein, this is prebiotic Bacterium is that bacillus licheniformis, lactic acid bacteria and Bifidobacterium press 1:1:What 1 ratio was mixed to get, fermentation temperature is 50 DEG C, when fermentation Between be 96h.After obtaining fermentation material, supernatant liquor and lower sediment are obtained after being centrifuged to the fermentation material, it is clear to upper layer Liquid is freeze-dried, and is obtained corresponding freeze-drying object, and dry with 35 DEG C of temperature to the lower sediment, is obtained Second mixture.
Again, 0.35g multi-vitamins are weighed and 0.1g phagostimulant is added in the freeze-drying object, is uniformly mixed, obtains Microcapsule granule is made to third mixture, and using the third mixture as core material.It wherein, is tieed up by 20g in multi-vitamins Raw element A, 3.7g vitamin B1, 4g vitamin B2, 2g vitamin B5, 4g vitamin B6, 11g vitamin C, 19g vitamin D3, 4g dimension Raw element E, 22g vitamin K3, 4g folic acid, 6g niacin and 0.3g biotin obtain after evenly mixing (wherein, multi-vitamins are pressed Illustrate the additional proportion of each vitamin constituent for 100g).Specifically, microcapsule granule the production method is as follows：Claim It takes 0.35g multi-vitamins and 0.1g phagostimulant to be added in the freeze-drying object, and is mixed, obtain third mixture； Using the third mixture as core material, according to the weight of the third mixture by core wall than 1:3 weigh the wall material of corresponding weight, wherein It with weight ratio is 1 that the wall material, which is gelatin and trehalose,:4 are mixed to get；The deionized water dissolving wall material is added, so that wall material is molten The concentration of liquid is 25%；Emulsifier and the third mixture are added in the wall material solution, it is after mixing evenly, equal using high pressure Matter machine carries out homogeneous, obtains homogeneous latex emulsion, wherein homogenization pressure 30MPa, mean time 4min；The homogeneous latex emulsion is carried out Spray drying, obtains microcapsule granule, wherein the condition of spray drying is：Inlet air temperature is 170 DEG C, and leaving air temp is 70 DEG C, Feed rate is 6mL/min.
Then, 26g corn flour, 16g wheat-middlings, 10g fish meal, 2.5g meat meal tankage, 2.5g fish oil, 0.3g chlorination gallbladder are weighed respectively Alkali, 0.2g salt, 2.0g shelling element, 2.0g composite mineral matter and 0.05g mould inhibitor are mixed, and 80 mesh are crossed after pulverization process Sieve, obtains the 4th mixture.Wherein, composite mineral matter is by 13g copper sulphate, 12g ferrous sulfate, 14g zinc lactate, 7.5g sulfuric acid Magnesium, 0.08g sodium selenite, 0.52g potassium iodide, 7.9g manganese sulfate and 45g calcium dihydrogen phosphate obtain after evenly mixing (wherein, Composite mineral matter is by the additional proportion for illustrating each mineral matter constituent for 100g).Then, the 4th mixture is put into In quality-adjusting device, be passed through that vapor is quenched, and will be conditioned after the 4th mixture enter extrusion granulator and pelletize, obtain To swelling granular.Specifically, the preparation process of swelling granular is：4th mixture is put into quality-adjusting device, being passed through temperature is 100 DEG C, quality be the 4th mixture gross mass the quenched 5min of 12% vapor, then will be conditioned after the 4th mixing Object 95 DEG C at a temperature of enter extrusion granulator pelletize, obtain swelling granular.
Finally, pressing 8 with microcapsule granule after swelling granular is dried with 100 DEG C of drying temperature:1 ratio into Row mixing, is improved the feed of Eriocheir sinensis anti-stress ability.
Embodiment three,
Firstly, cortex albiziae, Radix Codonopsis, the root bark of tree peony, Herba Andrographitis, radix scutellariae, Radix Angelicae Sinensis, wilsonii, folium isatidis, campanulaceae and dried orange peel are distinguished Pretreatment is dried, pretreatment temperature is 30 DEG C, pretreatment time 2h.Then it weighs respectively pretreated each through drying Chinese herbal medicine, specifically, weighing 10g cortex albiziae, 12g Radix Codonopsis, the 8g root bark of tree peony, 5g Herba Andrographitis, 11g radix scutellariae, 14g Radix Angelicae Sinensis, 15g thorn five Add, 10g folium isatidis, 8g campanulaceae and 7g dried orange peel, crushed, is uniformly mixed after crossing 60 meshes, traditional Chinese medicinal components are made.
It is mixed secondly, weighing 13g wheat bran, 12.5g dregs of beans, 4g rapeseed dregs and the above-mentioned mixed traditional Chinese medicinal components of 12g, The first mixture is obtained, and fermentation substrate is made according to the water that 2 times of weight are added in the weight of first mixture, then weigh 2.0g Probiotics is added in the fermentation substrate, is uniformly mixed and is placed in fermentation vat, ferments, obtain fermentation material.Wherein, the benefit Raw bacterium is that lactic acid bacteria and clostridium butyricum press 1:What 1 ratio was mixed to get, fermentation temperature is 55 DEG C, and fermentation time is for 24 hours.It is obtaining After fermentation materials, supernatant liquor and lower sediment are obtained after being centrifuged to the fermentation material, supernatant liquor is freeze-dried, Corresponding freeze-drying object is obtained, and the lower sediment is dried with 40 DEG C of temperature, obtains the second mixture.
Again, 0.1g multi-vitamins are weighed and 1.0g phagostimulant is added in the freeze-drying object, is uniformly mixed, obtains Third mixture, and microcapsule granule is made using the third mixture as core material.It wherein, is to be tieed up to give birth to by 15g in multi-vitamins Plain A, 2.3g vitamin B1, 8g vitamin B2, 4g vitamin B5, 3g vitamin B6, 7g vitamin C, 23g vitamin D3, 3g dimension life Plain E, 20g vitamin K3, 2g folic acid, 12g niacin and 0.7g biotin obtain after evenly mixing (wherein, multi-vitamins are pressed Illustrate the additional proportion of each vitamin constituent for 100g).Specifically, microcapsule granule the production method is as follows：Claim It takes 0.1g multi-vitamins and 1.0g phagostimulant to be added in the freeze-drying object, and is mixed, obtain third mixture；With The third mixture is core material, according to the weight of the third mixture by core wall than 1:3 weigh the wall material of corresponding weight, wherein should It with weight ratio is 1 that wall material, which is gelatin and trehalose,:2 are mixed to get；The deionized water dissolving wall material is added, so that wall material solution Concentration be 35%；Emulsifier and the third mixture are added in the wall material solution, after mixing evenly, use is high-pressure homogeneous Machine carries out homogeneous, obtains homogeneous latex emulsion, wherein homogenization pressure 50MPa, mean time 3min；The homogeneous latex emulsion is sprayed Mist is dry, obtains microcapsule granule, wherein the condition of spray drying is：Inlet air temperature is 160 DEG C, and leaving air temp is 70 DEG C, into Stream amount is 10mL/min.
Then, weigh respectively 20g corn flour, 14g wheat-middlings, 14g fish meal, 4g meat meal tankage, 1g fish oil, 0.2g choline chloride, 0.1g salt, 0.5g shelling element, 2.0g composite mineral matter and 0.1g mould inhibitor are mixed, and 80 meshes are crossed after pulverization process, Obtain the 4th mixture.Wherein, composite mineral matter be by 17g copper sulphate, 6g ferrous sulfate, 11g zinc lactate, 6.2g magnesium sulfate, (wherein, the grandidierite that 0.1g sodium selenite, 0.7g potassium iodide, 4.0g manganese sulfate and 55g calcium dihydrogen phosphate obtain after evenly mixing Substance is by the additional proportion for illustrating each mineral matter constituent for 100g).Then, the 4th mixture is put into quality-adjusting device In, be passed through that vapor is quenched, and will be conditioned after the 4th mixture enter extrusion granulator and pelletize, obtain extruding Particle.Specifically, the preparation process of swelling granular is：4th mixture is put into quality-adjusting device, being passed through temperature is 95 DEG C, matter Amount be the 4th mixture gross mass the quenched 7min of 12% vapor, then will be conditioned after the 4th mixture at 90 DEG C At a temperature of enter extrusion granulator pelletize, obtain swelling granular.
Finally, pressing 9 with microcapsule granule after swelling granular is dried with 100 DEG C of drying temperature:1 ratio into Row mixing, is improved the feed of Eriocheir sinensis anti-stress ability.
Example IV,
Firstly, cortex albiziae, Radix Codonopsis, the root bark of tree peony, Herba Andrographitis, radix scutellariae, Radix Angelicae Sinensis, wilsonii, folium isatidis, campanulaceae and dried orange peel are distinguished Pretreatment is dried, pretreatment temperature is 40 DEG C, pretreatment time 2.5h.Then it weighs respectively pretreated through drying Each Chinese herbal medicine, specifically, weighing 20g cortex albiziae, 10g Radix Codonopsis, the 12g root bark of tree peony, 7g Herba Andrographitis, 11g radix scutellariae, 10g Radix Angelicae Sinensis, 12g thorn Slender acanthopanax, 7g folium isatidis, 6g campanulaceae and 5g dried orange peel, are crushed, and are uniformly mixed after crossing 60 meshes, traditional Chinese medicinal components are made.
It is mixed secondly, weighing 7g wheat bran, 17.5g dregs of beans, 4g rapeseed dregs and the above-mentioned mixed traditional Chinese medicinal components of 10g, The first mixture is obtained, and fermentation substrate is made according to the water that 2 times of weight are added in the weight of first mixture, then weigh 1.5g Probiotics is added in the fermentation substrate, is uniformly mixed and is placed in fermentation vat, ferments, obtain fermentation material.Wherein, the benefit Raw bacterium is that bacillus licheniformis, Bifidobacterium and clostridium butyricum press 1:1:What 1 ratio was mixed to get, fermentation temperature is 45 DEG C, hair The ferment time is 120h.After obtaining fermentation material, supernatant liquor and lower sediment are obtained after being centrifuged to the fermentation material, to upper Layer clear liquid is freeze-dried, and obtains corresponding freeze-drying object, and dry with 30 DEG C of temperature to the lower sediment, Obtain the second mixture.
Again, 0.25g multi-vitamins are weighed and 0.1g phagostimulant is added in the freeze-drying object, is uniformly mixed, obtains Microcapsule granule is made to third mixture, and using the third mixture as core material.It wherein, is tieed up by 15g in multi-vitamins Raw element A, 2.3g vitamin B1, 8g vitamin B2, 4g vitamin B5, 3g vitamin B6, 7g vitamin C, 23g vitamin D3, 3g dimension Raw element E, 20g vitamin K3, 2g folic acid, (wherein, the multi-vitamins that 12g niacin and 0.7g biotin obtain after evenly mixing By the additional proportion for illustrating each vitamin constituent for 100g).Specifically, microcapsule granule the production method is as follows： It weighs 0.25g multi-vitamins and 0.1g phagostimulant is added in the freeze-drying object, and mixed, obtain third mixing Object；Using the third mixture as core material, according to the weight of the third mixture by core wall than 1:3 weigh the wall material of corresponding weight, It with weight ratio is 1 that wherein the wall material, which is gelatin and trehalose,:3 are mixed to get；The deionized water dissolving wall material is added, so that wall The concentration of material solution is 30%；Emulsifier is added in the wall material solution and the third mixture uses height after mixing evenly It presses homogenizer to carry out homogeneous, obtains homogeneous latex emulsion, wherein homogenization pressure 40MPa, mean time 5min；To the homogeneous latex emulsion It is spray-dried, obtains microcapsule granule, wherein the condition of spray drying is：Inlet air temperature is 150 DEG C, and leaving air temp is 60 DEG C, feed rate 8mL/min.
Then, weigh respectively 24g corn flour, 14g wheat-middlings, 12g fish meal, 3g meat meal tankage, 2g fish oil, 0.4g choline chloride, 0.2g salt, 1.5g shelling element, 2.5g composite mineral matter and 0.05g mould inhibitor are mixed, and 80 meshes are crossed after pulverization process, Obtain the 4th mixture.Wherein, composite mineral matter be by 15g copper sulphate, 10g ferrous sulfate, 12g zinc lactate, 5g magnesium sulfate, 0.06g sodium selenite, 0.44g potassium iodide, 7.5g manganese sulfate and 50g calcium dihydrogen phosphate obtain (wherein, compound after evenly mixing Minerals are by the additional proportion for illustrating each mineral matter constituent for 100g).Then, the 4th mixture is put into quenched In device, be passed through that vapor is quenched, and will be conditioned after the 4th mixture enter extrusion granulator and pelletize, obtain swollen Change particle.Specifically, the preparation process of swelling granular is：4th mixture is put into quality-adjusting device, be passed through temperature be 90 DEG C, Quality be the 4th mixture gross mass the quenched 7min of 15% vapor, then will be conditioned after the 4th mixture 90 Enter extrusion granulator at a temperature of DEG C to pelletize, obtains swelling granular.
Finally, pressing 8 with microcapsule granule after swelling granular is dried with 95 DEG C of drying temperature:1 ratio carries out Mixing, is improved the feed of Eriocheir sinensis anti-stress ability.
Embodiment five,
Firstly, cortex albiziae, Radix Codonopsis, the root bark of tree peony, Herba Andrographitis, radix scutellariae, Radix Angelicae Sinensis, wilsonii, folium isatidis, campanulaceae and dried orange peel are distinguished Pretreatment is dried, pretreatment temperature is 50 DEG C, pretreatment time 2.5h.Then it weighs respectively pretreated through drying Each Chinese herbal medicine, specifically, weighing 14g cortex albiziae, 11g Radix Codonopsis, the 15g root bark of tree peony, 9g Herba Andrographitis, 11g radix scutellariae, 12g Radix Angelicae Sinensis, 10g thorn Slender acanthopanax, 9g folium isatidis, 5g campanulaceae and 4g dried orange peel, are crushed, and are uniformly mixed after crossing 60 meshes, traditional Chinese medicinal components are made.
It is mixed, is obtained secondly, weighing 7g wheat bran, 15g dregs of beans, 2g rapeseed dregs and the above-mentioned mixed traditional Chinese medicinal components of 8g First mixture, and fermentation substrate is made according to the water that 2 times of weight are added in the weight of first mixture, then to weigh 1.0g prebiotic Bacterium is added in the fermentation substrate, is uniformly mixed and is placed in fermentation vat, ferments, obtain fermentation material.Wherein, the probiotics 1 is pressed for bacillus subtilis, lactic acid bacteria and clostridium butyricum:1:What 1 ratio was mixed to get, fermentation temperature is 50 DEG C, fermentation time For 96h.After obtaining fermentation material, supernatant liquor and lower sediment are obtained after being centrifuged to the fermentation material, to supernatant liquor It is freeze-dried, obtains corresponding freeze-drying object, and dry with 40 DEG C of temperature to the lower sediment, obtain Two mixtures.
Again, 0.4g multi-vitamins are weighed and 0.2g phagostimulant is added in the freeze-drying object, is uniformly mixed, obtains Third mixture, and microcapsule granule is made using the third mixture as core material.It wherein, is to be tieed up to give birth to by 18g in multi-vitamins Plain A, 1.6g vitamin B1, 6g vitamin B2, 2g vitamin B5, 4g vitamin B6, 10g vitamin C, 17g vitamin D3, 3g dimension life Plain E, 26g vitamin K3, 4g folic acid, 8g niacin and 0.4g biotin obtain after evenly mixing (wherein, multi-vitamins are pressed Illustrate the additional proportion of each vitamin constituent for 100g).Specifically, microcapsule granule the production method is as follows：Claim It takes 0.4g multi-vitamins and 0.2g phagostimulant to be added in the freeze-drying object, and is mixed, obtain third mixture；With The third mixture is core material, according to the weight of the third mixture by core wall than 1:3 weigh the wall material of corresponding weight, wherein should It with weight ratio is 1 that wall material, which is gelatin and trehalose,:4 are mixed to get；The deionized water dissolving wall material is added, so that wall material solution Concentration be 25%；Emulsifier and the third mixture are added in the wall material solution, after mixing evenly, use is high-pressure homogeneous Machine carries out homogeneous, obtains homogeneous latex emulsion, wherein homogenization pressure 40MPa, mean time 4min；The homogeneous latex emulsion is sprayed Mist is dry, obtains microcapsule granule, wherein the condition of spray drying is：Inlet air temperature is 170 DEG C, and leaving air temp is 60 DEG C, into Stream amount is 6mL/min.
Then, 28g corn flour, 16g wheat-middlings, 14g fish meal, 2.5g meat meal tankage, 1.5g fish oil, 0.2g chlorination gallbladder are weighed respectively Alkali, 0.1g salt, 1.0g shelling element, 3.0g composite mineral matter and 0.1g mould inhibitor are mixed, and 80 mesh are crossed after pulverization process Sieve, obtains the 4th mixture.Wherein, composite mineral matter is by 7g copper sulphate, 12g ferrous sulfate, 15g zinc lactate, 6.5g sulfuric acid Magnesium, 0.1g sodium selenite, 0.4g potassium iodide, 9.0g manganese sulfate and 50g calcium dihydrogen phosphate obtain (wherein, multiple after evenly mixing Minerals are closed by the additional proportion for illustrating each mineral matter constituent for 100g).Then, the 4th mixture is put into tune In matter device, be passed through that vapor is quenched, and will be conditioned after the 4th mixture enter extrusion granulator and pelletize, obtain Swelling granular.Specifically, the preparation process of swelling granular is：4th mixture is put into quality-adjusting device, being passed through temperature is 90 DEG C, quality be the 4th mixture gross mass the quenched 5min of 15% vapor, then will be conditioned after the 4th mixture 95 DEG C at a temperature of enter extrusion granulator pelletize, obtain swelling granular.
Finally, pressing 7 with microcapsule granule after swelling granular is dried with 105 DEG C of drying temperature:1 ratio into Row mixing, is improved the feed of Eriocheir sinensis anti-stress ability.
Embodiment six,
Firstly, cortex albiziae, Radix Codonopsis, the root bark of tree peony, Herba Andrographitis, radix scutellariae, Radix Angelicae Sinensis, wilsonii, folium isatidis, campanulaceae and dried orange peel are distinguished Pretreatment is dried, pretreatment temperature is 30 DEG C, pretreatment time 3h.Then it weighs respectively pretreated each through drying Chinese herbal medicine, specifically, weighing 10g cortex albiziae, 14g Radix Codonopsis, the 10g root bark of tree peony, 5g Herba Andrographitis, 17g radix scutellariae, 10g Radix Angelicae Sinensis, 10g thorn five Add, 12g folium isatidis, 6g campanulaceae and 6g dried orange peel, crushed, is uniformly mixed after crossing 60 meshes, traditional Chinese medicinal components are made.
It is mixed, is obtained secondly, weighing 5g wheat bran, 10g dregs of beans, 6g rapeseed dregs and the above-mentioned mixed traditional Chinese medicinal components of 14g Fermentation substrate is made to the first mixture, and according to the water that 2 times of weight are added in the weight of first mixture, then weighs 1.0g benefit Raw bacterium is added in the fermentation substrate, is uniformly mixed and is placed in fermentation vat, ferments, obtain fermentation material.Wherein, this is prebiotic Bacterium is that bacillus licheniformis and Bifidobacterium press 1:What 2 ratios were mixed to get, fermentation temperature is 40 DEG C, fermentation time 72h.? After obtaining fermentation material, supernatant liquor and lower sediment are obtained after being centrifuged to the fermentation material, supernatant liquor is freezed It is dry, corresponding freeze-drying object is obtained, and dry with 30 DEG C of temperature to the lower sediment, obtains the second mixing Object.
Again, 0.5g multi-vitamins are weighed and 0.2g phagostimulant is added in the freeze-drying object, is uniformly mixed, obtains Third mixture, and microcapsule granule is made using the third mixture as core material.It wherein, is to be tieed up to give birth to by 15g in multi-vitamins Plain A, 4.5g vitamin B1, 3g vitamin B2, 5g vitamin B5, 3g vitamin B6, 13g vitamin C, 15g vitamin D3, 3g dimension life Plain E, 20g vitamin K3, 8g folic acid, 10g niacin and 0.5g biotin obtain after evenly mixing (wherein, multi-vitamins are pressed Illustrate the additional proportion of each vitamin constituent for 100g).Specifically, microcapsule granule the production method is as follows：Claim It takes 0.5g multi-vitamins and 0.2g phagostimulant to be added in the freeze-drying object, and is mixed, obtain third mixture；With The third mixture is core material, according to the weight of the third mixture by core wall than 1:3 weigh the wall material of corresponding weight, wherein should It with weight ratio is 1 that wall material, which is gelatin and trehalose,:2 are mixed to get；The deionized water dissolving wall material is added, so that wall material solution Concentration be 20%；Emulsifier and the third mixture are added in the wall material solution, after mixing evenly, use is high-pressure homogeneous Machine carries out homogeneous, obtains homogeneous latex emulsion, wherein homogenization pressure 30MPa, mean time 6min；The homogeneous latex emulsion is sprayed Mist is dry, obtains microcapsule granule, wherein the condition of spray drying is：Inlet air temperature is 150 DEG C, and leaving air temp is 80 DEG C, into Stream amount is 10mL/min.
Then, weigh respectively 28g corn flour, 12g wheat-middlings, 12g fish meal, 3g meat meal tankage, 3g fish oil, 0.5g choline chloride, 0.2g salt, 2.0g shelling element, 2.5g composite mineral matter and 0.1g mould inhibitor are mixed, and 80 meshes are crossed after pulverization process, Obtain the 4th mixture.Wherein, composite mineral matter be by 17g copper sulphate, 8g ferrous sulfate, 8g zinc lactate, 3g magnesium sulfate, 0.04g sodium selenite, 0.46g potassium iodide, 8.5g manganese sulfate and 55g calcium dihydrogen phosphate obtain (wherein, compound after evenly mixing Minerals are by the additional proportion for illustrating each mineral matter constituent for 100g).Then, the 4th mixture is put into quenched In device, be passed through that vapor is quenched, and will be conditioned after the 4th mixture enter extrusion granulator and pelletize, obtain swollen Change particle.Specifically, the preparation process of swelling granular is：4th mixture is put into quality-adjusting device, being passed through temperature is 100 DEG C, quality be the 4th mixture gross mass the quenched 7min of 8% vapor, then will be conditioned after the 4th mixture exist Enter extrusion granulator at a temperature of 100 DEG C to pelletize, obtains swelling granular.
Finally, pressing 7 with microcapsule granule after swelling granular is dried with 105 DEG C of drying temperature:1 ratio into Row mixing, is improved the feed of Eriocheir sinensis anti-stress ability.
The present invention provides a kind of feed and preparation method thereof for improving Eriocheir sinensis anti-stress ability, the composition of the feed For：It is corn flour, wheat-middlings, wheat bran, fish meal, dregs of beans, rapeseed dregs, meat meal tankage, fish oil, choline chloride, salt, traditional Chinese medicinal components, prebiotic Bacterium, shelling element, multi-vitamins, composite mineral matter, phagostimulant and mould inhibitor.Wheat bran, dregs of beans, rapeseed dregs and traditional Chinese medicinal components are pressed It is mixed after ratio weighing, obtains the first mixture, fermentation substrate is made in the water that corresponding weight is added；In proportion by probiotics It is added in fermentation substrate after weighing, ferments after mixing, obtain fermentation material；On being obtained after being centrifuged to fermentation material Layer clear liquid and lower sediment, are freeze-dried supernatant liquor, obtain corresponding freeze-drying object, and carry out to lower sediment Drying, obtains the second mixture；It mixes, obtains with freeze-drying object after multi-vitamins and phagostimulant are weighed in proportion Third mixture, and microcapsule granule is made using third mixture as core material；By corn flour, wheat-middlings, fish meal, meat meal tankage, fish oil, Choline chloride, salt, shelling element, composite mineral matter and mould inhibitor are mixed after weighing in proportion, and 80 are crossed after pulverization process Mesh obtains the 4th mixture；4th mixture is put into quality-adjusting device, is passed through that vapor is quenched, and will be conditioned after Four mixtures enter extrusion granulator and pelletize, and obtain swelling granular；After swelling granular is dried, with microcapsule granule It is mixed by preset ratio, is improved the feed of Eriocheir sinensis anti-stress ability.By the above-mentioned means, can reach following Beneficial effect：
1) traditional Chinese medicinal components with raising anti-stress ability are added in feed, can be improved by the feeding feed The anti-stress ability of magnificent Eriocheir, the traditional Chinese medicinal components can also enhance combined immune power of Eriocheir sinensis etc., can effectively reduce The death rate can further improve herbal medicine efficacy, and can in addition, ferment to traditional Chinese medicinal components by probiotics fermention technology It eliminates Chinese medicine toxic side effect and changes herbal nature；
2) it is fermented by probiotics to wheat bran, dregs of beans, rapeseed dregs, can effectively reduce dregs of beans, the nocuousness in rapeseed dregs Substance improves the safety of feed, meanwhile, after probiotics fermention, the substance that a part is difficult to digest and assimilate can be become Simple substance easy to digest and absorb, improves the nutritive value of feed, promotes the digestion and absorption of Eriocheir sinensis, accelerate its life Long speed can also convert the small-molecule substances such as monosaccharide and oligosaccharides for the macromolecular substances that cellulose, pectic substance etc. are difficult to degrade, And generate a variety of organic acids, micro-element, biological enzyme and a variety of growth factors, greatly improve institute's fermentation materials trophic level and Digestive utilization ratio reduces feeding cost to improve efficiency of feed utilization, improves breeding income；
3) by prebiotic fermentation technique and microcapsules technology, so that contain a large amount of beneficial microbe viable bacteria in feed, it can The intestinal microecology balance for establishing Eriocheir sinensis, adjusts its stomach balance, to promote nutrient absorption, improves feed conversion Rate, meanwhile, probiotics and some active materials are made by microcapsule granule by microcapsules technology, high temperature is can avoid and kills part The benefit materials such as probiotics and destruction protein active agent；
4) it is added in the growth and development process of Eriocheir sinensis and maintains dimension life necessary to eubolism and physiological function Element and minerals, can be improved the nutrition full price and utilization rate of feed, enhance the anti-stress ability of Eriocheir sinensis；
5) raw material sources required for the present invention are extensive, safe and non-toxic, cheap, are suitble to large-scale popularization, and fermented Feed afterwards, the holding time is longer, has the stronger market competitiveness.
Improve the verification test of Eriocheir sinensis anti-stress ability
If other conditions are the same, 7 groups of tests, including control group, test group 1, test group 2, test group are set 3, test group 4, test group 5 and test group 6, wherein control group feeds Eriocheir sinensis, examination using common Chinese eriocheir sinensises feed Test the raising Eriocheir sinensia that group 1-6 group is prepared using first embodiment to sixth embodiment in embodiment in the present invention respectively The forage feed Eriocheir sinensis of crab anti-stress ability, remaining feeding and management condition are identical.Through feeding after a period of time After supporting, the death rate of the Chinese huge legendary turtle suede crab of 7 groups of tests is counted, as shown in table 1.
The death rate in test group 1-6 it can be seen from the data in table 1 is declined compared to control group, In, the death rate of test group 4 is minimum, it can be seen that, the Feed Energy provided by the invention for improving Eriocheir sinensis anti-stress ability The anti-stress ability for enough improving Eriocheir sinensis strengthens its survival rate to reduce its death rate.
Control group Test group 1 Test group 2 Test group 3 Test group 4 Test group 5 Test group 6
Death rate % 27.5 15.2 11.8 14.3 10.7 12.4 13.6
Table 1
The above is only a preferred embodiment of the present invention, is not intended to limit the scope of the invention, all to utilize this hair Equivalent structure or equivalent flow shift made by bright specification and accompanying drawing content is applied directly or indirectly in other relevant skills Art field, is included within the scope of the present invention.

Claims (10)

1. a kind of feed for improving Eriocheir sinensis anti-stress ability, which is characterized in that in terms of weight percentage, the raising The group of the feed of Eriocheir sinensis anti-stress ability becomes：Corn flour 18%-28%, wheat-middlings 12%-20%, wheat bran 5%- 13%, fish meal 8%-14%, dregs of beans 10%-20%, rapeseed dregs 2%-6%, meat meal tankage 2%-4%, fish oil 1%-3%, chlorination Choline 0.2%-0.6%, salt 0.1%-0.4%, traditional Chinese medicinal components 6%-14%, probiotics 0.5%-2%, shelling element 0.5%- 2%, multi-vitamins 0.1%-0.5%, composite mineral matter 1%-3%, phagostimulant 0.1%-0.2% and mould inhibitor 0.05%- 0.1%.
2. improving the feed of Eriocheir sinensis anti-stress ability as described in claim 1, which is characterized in that the traditional Chinese medicinal components Composition and weight percent it is as follows：Cortex albiziae 10%-20%, Radix Codonopsis 9%-14%, root bark of tree peony 8%-15%, Herba Andrographitis 5%- 12%, radix scutellariae 11%-17%, Radix Angelicae Sinensis 10%-16%, wilsonii 10%-15%, folium isatidis 6%-12%, campanulaceae 5%-9% and Dried orange peel 4%-7%.
3. improving the feed of Eriocheir sinensis anti-stress ability as described in claim 1, which is characterized in that the prebiotic bacterium bag Include at least two of bacillus licheniformis, bacillus subtilis, lactic acid bacteria, Bifidobacterium and clostridium butyricum.
4. improving the feed of Eriocheir sinensis anti-stress ability as described in claim 1, which is characterized in that the compound dimension life The composition and weight percent of element are as follows：Vitamin A 15%-22%, vitamin B10.5%-4.5%, vitamin B23%- 8%, vitamin B52%-5%, vitamin B63%-6%, vitamin C 7%-13%, vitamin D315%-23%, dimension life Plain E 3%-7%, vitamin K320%-28%, folic acid 2%-8%, niacin 5%-12% and biotin 0.3%-0.7%.
5. improving the feed of Eriocheir sinensis anti-stress ability as described in claim 1, which is characterized in that the complex mineral The composition and weight percent of matter are as follows：Copper sulphate 7%-17%, ferrous sulfate 6%-12%, zinc lactate 8%-15%, sulfuric acid Magnesium 3%-8%, sodium selenite 0.04%-0.1%, potassium iodide 0.3%-0.7%, manganese sulfate 4%-9% and calcium dihydrogen phosphate 45%-55%.
6. a kind of preparation side of the feed according to any one of claims 1 to 5 for improving Eriocheir sinensis anti-stress ability Method, which is characterized in that the preparation method comprises the following steps：
S1：It is mixed after wheat bran, dregs of beans, rapeseed dregs and traditional Chinese medicinal components are weighed in proportion, obtains the first mixture, according to institute Fermentation substrate is made in the water for stating weight 2 times of weight of addition of the first mixture；
S2：It is added to after probiotics is weighed in proportion in the fermentation substrate, ferments after mixing, fermented Object, wherein fermentation temperature is 35 DEG C -55 DEG C, and fermentation time is -120h for 24 hours；
S3：Supernatant liquor and lower sediment are obtained after being centrifuged to the fermentation material, it is dry to carry out freezing to the supernatant liquor It is dry, corresponding freeze-drying object is obtained, and dry with 30 DEG C -40 DEG C of temperature to the lower sediment, it is mixed to obtain second Close object；
S4：It is mixed after multi-vitamins and phagostimulant are weighed in proportion with the freeze-drying object, obtains third mixing Object, and microcapsule granule is made using the third mixture as core material；
S5：By corn flour, wheat-middlings, fish meal, meat meal tankage, fish oil, choline chloride, salt, shelling element, composite mineral matter and mould inhibitor It is mixed after weighing in proportion, 80 meshes is crossed after pulverization process, obtain the 4th mixture；
S6：4th mixture is put into quality-adjusting device, is passed through that vapor is quenched, and will be conditioned after the 4th mixture into Enter extrusion granulator to pelletize, obtains swelling granular；
S7：After swelling granular drying, is mixed, obtained in the raising by preset ratio with the microcapsule granule The feed of magnificent Eriocheir anti-stress ability.
7. improving the preparation method of the feed of Eriocheir sinensis anti-stress ability as claimed in claim 6, which is characterized in that It is further comprising the steps of before step S1：
S8：Cortex albiziae, Radix Codonopsis, the root bark of tree peony, Herba Andrographitis, radix scutellariae, Radix Angelicae Sinensis, wilsonii, folium isatidis, campanulaceae and dried orange peel are done respectively Dry pretreatment, wherein pretreatment temperature is 30 DEG C -50 DEG C, pretreatment time 2h-3h；
S9：Taking weight percent content is respectively 10%-20% cortex albiziae, 9%-14% Radix Codonopsis, the 8%-15% root bark of tree peony, 5%- 12% Herba Andrographitis, 11%-17% radix scutellariae, 10%-16% Radix Angelicae Sinensis, 10%-15% wilsonii, 6%-12% folium isatidis, 5%-9% Campanulaceae and 4%-7% dried orange peel, are crushed, and are uniformly mixed after crossing 60 meshes, the traditional Chinese medicinal components are made.
8. improving the preparation method of the feed of Eriocheir sinensis anti-stress ability as claimed in claim 6, which is characterized in that step Suddenly S4 includes：
S41：It is mixed after multi-vitamins and phagostimulant are weighed in proportion with the freeze-drying object, obtains third mixing Object；
S42：Using the third mixture as core material, according to the weight of the third mixture by core wall than 1:3 weigh corresponding weight The wall material of amount, wherein it with weight ratio is 1 that the wall material, which is gelatin and trehalose,:2-1:5 are mixed to get；
S43：Wall material described in deionized water dissolving is added, so that the concentration of wall material solution is 20%-35%；
S44：Emulsifier is added in the wall material solution and the third mixture uses high pressure homogenizer after mixing evenly Homogeneous is carried out, obtains homogeneous latex emulsion, wherein homogenization pressure 20MPa-50MPa, mean time 3min-7min；
S45：The homogeneous latex emulsion is spray-dried, microcapsule granule is obtained, wherein the condition of spray drying is：Air inlet Temperature is 150 DEG C -170 DEG C, and leaving air temp is 60 DEG C -80 DEG C, feed rate 6mL/min-10mL/min.
9. improving the preparation method of the feed of Eriocheir sinensis anti-stress ability as claimed in claim 8, which is characterized in that step Suddenly S8 includes：
S81：4th mixture is put into quality-adjusting device, be passed through temperature be 90 DEG C -100 DEG C, quality be it is described 4th mixing The quenched 5min-9min of the vapor of the 8%-15% of object gross mass；
S82：The 4th mixture after will be conditioned 90 DEG C -100 DEG C at a temperature of enter extrusion granulator and pelletize, Obtain swelling granular.
10. improving the preparation method of the feed of Eriocheir sinensis anti-stress ability as claimed in claim 6, which is characterized in that In step s 9, the drying temperature is 95 DEG C -105 DEG C, and the preset ratio is 7:1-9:1.
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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110235822A (en) * 2019-07-10 2019-09-17 湖北省农业科学院农产品加工与核农技术研究所 Living aquatic product Antistress agent and its production method and application method
CN114223809A (en) * 2021-12-09 2022-03-25 南京宝辉生物饲料有限公司 Fermented feed for crabs as well as preparation method and application of fermented feed

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110235822A (en) * 2019-07-10 2019-09-17 湖北省农业科学院农产品加工与核农技术研究所 Living aquatic product Antistress agent and its production method and application method
CN114223809A (en) * 2021-12-09 2022-03-25 南京宝辉生物饲料有限公司 Fermented feed for crabs as well as preparation method and application of fermented feed

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Application publication date: 20181116