CN114617039B - Method for cultivating root-segment-growing or breeding salvia miltiorrhiza - Google Patents
Method for cultivating root-segment-growing or breeding salvia miltiorrhiza Download PDFInfo
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Abstract
The invention relates to a method for cultivating root-segment-cultivated or propagation salvia miltiorrhiza. In the process of cultivating the salvia miltiorrhiza bunge, firstly, salvia miltiorrhiza bunge cultivation column pots with the root systems at the middle and lower parts and the soil which are ventilated and water-permeable are manufactured, and then, the three layers of substrates are filled for respectively rejuvenating and strengthening seedlings, stimulating the occurrence of lateral roots of salvia miltiorrhiza bunge and promoting the extension of the root systems. Compared with the common root segment propagation and seed propagation modes of the field, the root length, the number of segments capable of producing roots, the propagation capacity, the number of seeds (seedlings) produced per mu, the propagation coefficient and the like of the method have obvious increase.
Description
Technical Field
The invention relates to a method for cultivating root-segment-growing seedlings or propagating salvia miltiorrhiza, belonging to the technical field of traditional Chinese medicine cultivation.
Background
The salvia miltiorrhiza is grown on sunny hillside grasses, ditch sides, roadside or forest sides and the like, and is distributed in most regions in China. The salvia miltiorrhiza planting mainly adopts two breeding modes, one mode is seed breeding, and the other mode is root section breeding. Salvia miltiorrhiza propagated by seeds can be harvested after 2-3 years generally, and can be harvested after 1-2 years of root segment propagation, and can also be harvested if management measures keep up with 1 year, so root segment propagation is the main point in production. The root segment propagation of salvia miltiorrhiza mainly utilizes the germination characteristics of salvia miltiorrhiza roots, selects salvia miltiorrhiza strips with certain specifications, cuts the salvia miltiorrhiza strips into root segments with certain length for seedling cultivation, and has the main problems of degeneration of the salvia miltiorrhiza used for root segment propagation, germplasm hybridization, reduction of vitality, uneven specification and quality of the root segments, low emergence rate and low propagation coefficient.
At present, the detoxification and rapid propagation technology of salvia miltiorrhiza is basically mature, but the production and application of tissue rapid propagation seedlings of salvia miltiorrhiza are limited by the propagation technology. The conventional breeding mode is field breeding or simple facility (seedling bed and the like) breeding, the two breeding modes have the defects of extensive breeding technology, simple and crude breeding conditions and low breeding coefficient, and also have the defects of rapid degeneration of stress resistance and high yield, so that the virus-free advantage utilization period is short, the benefit is low, and the popularization is difficult.
Disclosure of Invention
Aiming at the problems, the invention provides a method for cultivating root-segment seedlings or propagating salvia miltiorrhiza. The method comprises the steps of rapid propagation of salvia miltiorrhiza seeds, establishment of a detoxified tissue culture seedling group, propagation of salvia miltiorrhiza seeds in a root section of a column pot, seedling culture in the root section and field transplantation. Compared with the common root segment propagation and seed propagation modes of the field, the root length, the number of segments capable of producing roots, the propagation capacity, the number of seeds (seedlings) produced per mu, the propagation coefficient and the like of the method have obvious increase.
The technical scheme of the invention is as follows: a method for cultivating root-segment seedlings or root-segment seedlings of red sage root includes such steps as quick reproduction of red sage root, creating detoxicated tissue culture seedlings, cultivating root of red sage root, culturing root-segment seedlings and transplanting in field,
the method for cultivating the salvia miltiorrhiza seeds comprises the following steps:
(1) Making a red sage root cultivating column basin (cylindrical flowerpot) with a height of 55-65cm, an inner diameter of 15-20cm, and ventilating and water-leaking holes arranged on the basin wall and the bottom of the basin at positions 7-8cm and 15-16cm away from the bottom of the basin; a ventilating and water-permeating pipe with the diameter of 1.0-1.2cm is arranged in the center of the column pot, and the height of the ventilating and water-permeating pipe is equal to that of the column pot, so that the ventilating and water-permeating of the root system-soil at the middle lower part of the pot is facilitated, and the influence of accumulated water and waterlogging on the growth of the root system is prevented;
(2) Preparing a bacon matrix and filling in layers: distribution of matrix layer of the column basin (the following parts are calculated by volume parts):
seedling culture layer (5-7 cm): 0.8 to 1.2 parts of sphagnum moss, 0.8 to 1.2 parts of edible fungus residues and 0.8 to 1.2 parts of fine sand, which is favorable for seedling revival and strong seedling cultivation;
lateral root meristematic layer (5-7 cm): 0.8 to 1.2 parts of vermiculite, 1.8 to 2.2 parts of coarse sand, 0.8 to 1.2 parts of sphagnum and 0.8 to 1.2 parts of edible fungus dregs, which are favorable for stimulating the generation of lateral roots of salvia miltiorrhiza and increasing the number of roots;
root elongation layer (45-50 cm): 2.5 to 3.5 parts of sphagnum moss, 2.5 to 3.5 parts of edible fungus dregs, 1.8 to 2.2 parts of vermiculite, 0.8 to 1.2 parts of perlite and 0.8 to 1.2 parts of fine sand, which are beneficial to the elongation and the downward binding of root systems, and the use of the fungus dregs can ensure that strong seedlings can flourish without overgrowth and premature senility, and is beneficial to obtaining strong root systems;
(3) Transplanting: transplanting the refined tissue culture seedlings (in late 3-month ten days) into column pots, one seedling in each pot;
(4) Water regulation and root promotion: the water content in the culture medium is controlled to be 70-75%, the water content in the column pot is gradually reduced from bottom to top along with the downward binding of the root system, and water accumulation in the pot is strictly prevented; after seedling is slowed down, water is sprayed once in 3-5 days generally according to the water change in the column basin, and water accumulation in the basin is prevented strictly;
(5) Removing flowers and increasing roots: removing flower moss after the salvia miltiorrhiza blooms, and promoting the growth of root systems;
(6) Direction-regulating hair roots: stopping water management when the overground part begins to wither, pouring the column pot in the vertical direction at an angle of 30-45 degrees, standing for 10-20 days to promote redistribution of hormones at different parts in the root so as to be beneficial to generation of lateral roots or adventitious roots, and harvesting from the late 10 months to the early 3 months of the next year; 5-7 strips with root thickness of 0.6-1.0 cm and root length of 50-60 cm can be obtained in each basin.
Further, the rapid propagation of salvia miltiorrhiza seeds and establishment of the detoxified tissue culture seedling group comprises the steps of selecting excellent salvia miltiorrhiza seeds, carrying out tissue culture and rapid propagation, establishing the salvia miltiorrhiza detoxified tissue culture seedling group, and then hardening seedlings.
Further, the root segment seedling culture comprises the following steps: cutting the root strips into 3-5cm root segments, performing facility pregermination and seedling raising (starting in 2 months of the next year) to generate callus, wherein spores are not expanded.
Further, the field transplanting is as follows: ridging (ten days in 3 months to 4 months in the next year), planting in proper soil moisture, and conventionally planting after film covering.
Preferably, the height of the column basin is 60cm, and a ventilation and seepage pipe with the diameter of 1.2cm is arranged in the center of the column basin.
Preferably, the distribution of the column basin matrix layer (the following parts are calculated by weight):
a seedling culture layer (5 cm), 1 part of sphagnum moss, 1 part of edible fungus residues and 1 part of fine sand;
1 part of vermiculite, 2 parts of coarse sand, 1 part of sphagnum moss and 1 part of edible fungus residue;
root elongation layer (50 cm): 3 parts of sphagnum moss, 3 parts of edible fungus residues, 2 parts of vermiculite, 1 part of perlite and 1 part of fine sand.
Preferably, the design of the ventilation and seepage holes on the wall of the column basin is as follows: two rows of small holes are respectively arranged at the positions 7-8cm and 15-16cm away from the bottom of the column basin, 4 holes are uniformly distributed in each row around the basin wall, the relative positions of the two rows of holes are arranged in a vertical crossing manner, and the hole diameters are 0.4-0.6cm. The design of the bottom ventilation and seepage hole: the bottom of the column basin is uniformly provided with 4 holes with the aperture of 0.8-1.2cm. The middle part is ventilated and is penetrated the water pipe and is set up: the bottom end of the ventilation and seepage pipe is connected with the bottom of the column basin, and a hole is formed; the pipe wall of the ventilation and seepage pipe is provided with 2 rows of small holes in the longitudinal direction, the longitudinal distance between the holes is 8-10cm, the two rows of small holes are oppositely and crossly arranged, and the aperture is 0.2-0.3cm. The diameters of the holes are diameters.
The technical principle of the invention is as follows:
1. the salvia miltiorrhiza detoxified tissue culture seedlings have remarkable advantages of yield, quality and resistance in production, and the advantages of the salvia miltiorrhiza detoxified tissue culture seedlings weaken along with the increase of field propagation algebra, so that the specially designed column pot root section propagation is adopted to replace field propagation or facility propagation after the salvia miltiorrhiza strains are rapidly propagated to establish the detoxified tissue culture seedling group, thereby greatly improving the propagation coefficient and the like;
2. the salvia miltiorrhiza is a deep-rooted plant and mainly comprises adventitious roots, the length of the root system is influenced by the restriction of a soil substrate layer and permeability in field production, and the occurrence of the adventitious roots is closely related to the texture, nutrition and ventilation conditions of a surface soil layer. The growth and development of the root system have geotropism, water taxis, fertilizer taxis and gas taxis. Adjustment of the root direction affects the hormone distribution in the root system, and thus affects the occurrence of lateral roots or spores. According to the principle, firstly, a salvia miltiorrhiza seed root cultivation column pot with a middle-lower root system and soil which are ventilated and water-permeable is manufactured, then the substrate is filled in three layers, and a seedling cultivation layer is favorable for seedling revival and strong seedling cultivation; the lateral root meristematic layer is beneficial to stimulating the generation of the lateral root of the salvia miltiorrhiza, and the number of the root is increased; the root system elongation layer is beneficial to the elongation and the downward binding of the root system, the use of the fungus dregs can ensure that strong seedlings can be vigorous without overgrowth and premature senility, and the strong root system can be obtained.
The invention has the technical effects that: after the trunk pot root segment propagation is adopted, compared with the common root segment propagation and seed propagation modes of the field, the root length, the number of segments capable of producing roots, the propagation capacity, the number of seeds (seedlings) produced per mu, the propagation coefficient and the like are obviously increased.
Drawings
FIG. 1 is a schematic view of the structure of a pot for cultivating salvia miltiorrhiza seeds.
Detailed Description
The present invention will be described in further detail with reference to the following examples and drawings. As shown in fig. 1: 1) The overall design is as follows: the height of the column basin is 55-65cm, and the inner diameter is 15-20cm; a ventilating and water-permeating pipe with the diameter of 1.0-1.2cm is arranged in the center of the column basin; 2) The design of the ventilation and seepage holes on the wall of the column basin: two rows of small holes are respectively arranged at the positions 7-8cm and 15-16cm away from the bottom of the column basin, 4 holes are uniformly distributed in each row around the basin wall, the relative positions of the two rows of holes are arranged in a vertical crossing manner, and the hole diameters are 0.4-0.6cm; 3) The design of the bottom ventilation and water seepage hole: the bottom of the column basin is uniformly provided with 4 holes with the aperture of 0.8-1.2cm; 4) The middle part is ventilated and is penetrated the water pipe and is set up: the bottom end of the ventilation and seepage pipe is connected with the bottom of the column basin, and holes are formed; the pipe wall of the ventilation and seepage pipe is provided with 2 rows of small holes in the longitudinal direction, the longitudinal distance between the holes is 8-10cm, the two rows of small holes are oppositely and crossly arranged, and the aperture is 0.2-0.3cm.
Example 1
1. Selecting excellent individual plants of the Luyuan Salvia miltiorrhiza No. 1 variety, utilizing plant tips in 6 months in 2019, performing tissue culture and rapid propagation, establishing 2000 virus-free and rapid propagation groups of the excellent plants, and hardening off seedlings in 3 middle ten days in 2020 year;
2. customizing a column pot for cultivating salvia miltiorrhiza seeds. The height of the column basin is 60cm, the inner diameter is 15-20cm, and ventilating and water-seepage holes (as shown in figure 1) are arranged on the basin wall and the bottom of the column basin at positions 7.5cm and 15cm away from the bottom of the column basin; a ventilating and water-permeating pipe with the diameter of 1.2cm is arranged in the center of the column basin and is as high as the column basin, so that the ventilating and water-permeating of the root system-soil at the middle lower part of the basin is facilitated, and the influence of accumulated water and waterlogging on the growth of the root system is prevented;
3. preparing substrate, loading into pots in layers
Matrix components: sphagnum, edible fungus dregs, vermiculite, perlite, fine sand and the like;
column basin matrix layer distribution (the following parts are parts by volume):
a seedling culture layer (5 cm) is formed by 1 part of sphagnum moss, 1 part of edible fungus residues and 1 part of fine sand, so that the seedling is favorable for recovering seedlings and culturing strong seedlings;
the root system comprises a lateral root meristem layer (5 cm), 1 part of vermiculite, 2 parts of coarse sand, 1 part of sphagnum moss and 1 part of edible fungus residue, and is beneficial to stimulating the generation of lateral roots of the salvia miltiorrhiza and increasing the number of roots;
root elongation layer (50 cm): 3 parts of sphagnum moss, 3 parts of edible fungus dregs, 2 parts of vermiculite, 1 part of perlite and 1 part of fine sand and the like, so that the root system can be stretched and pricked, strong seedlings can be grown without overgrowth and premature senility due to the use of the fungus dregs, and the strong root system can be obtained.
Firstly, installing a root system elongation layer, and then installing a lateral root meristem layer on the root system elongation layer; finally, a seedling culture layer is arranged on the uppermost layer, compacted and watered thoroughly;
4. transplanting tissue culture seedlings: transplanting the refined tissue culture seedlings into culture column pots, namely No. 3 and No. 28 in 2020, one seedling in each pot;
5. water content management: the water content in the bacon substrate is generally controlled to be 70-75%, and the water content in the column pot is gradually reduced from bottom to top along with the downward binding of the root system; after seedling buffering, water is sprayed once in 3-5 days generally according to the water change in the column basin, and water accumulation in the basin is prevented strictly;
6. coating and removing flowers: in the middle and late ten days of 6 months in 2020, the salvia miltiorrhiza begin to bolt and flower, and flower and moss are removed in time to promote the growth of root systems;
7. adjusting the direction of the column basin: and in 25 days after 9 and 25 months in 2020, stopping water management when the overground part begins to wither, and completely laying the column basin at an angle of 30-45 degrees towards the sunny direction for 5 days after 10 and 5 months to promote redistribution of hormones at different parts in the roots so as to facilitate generation of lateral roots or adventitious roots.
The field effect pairs for example 1 and field root section propagation (using conventional techniques currently in use) and seed propagation (using conventional techniques currently in use) are shown in table 1. As can be seen from table 1: by adopting the column pot root section propagation, the average single root length, the average single plant root length, the number of root sections (counted by 5cm of root section length) producible by each plant, the propagation capacity (plant) per mu, the number of seeds (seedlings) produced per mu and the propagation coefficient (counted by 6000 plants per mu) are all obviously improved.
TABLE 1 comparison of field Effect of root segment propagation and seed propagation in example 1 and the field
The root segment may perform the following operations:
root-section seedling culture: cutting into 3-5cm pieces, and performing facility germination and seedling raising (beginning in late 2 months of the next year) to generate callus, wherein spores do not swell.
Transplanting in the field: ridging (3 months, middle ten days-4 months, last ten days) in the next year, planting in proper soil moisture, covering with film, and continuously planting Salvia miltiorrhiza Bunge according to conventional method until harvesting.
Example 2
1. Selecting good single plant of Luyuan Salvia miltiorrhiza No. 1 variety, performing tissue culture and rapid propagation by using the plant bud tip in the first 7 th of 2020, establishing 3000 virus-free rapid propagation groups of good plant lines, and hardening off seedlings in the last 3 rd ten days of 2021;
2. customizing a root cultivation column basin of the salvia miltiorrhiza: the height of the column basin is 60cm, the inner diameter is 15-20cm, and ventilating and water-seepage holes (as shown in figure 1) are arranged on the basin wall and the bottom of the column basin at positions 7.5cm and 15cm away from the bottom of the column basin; a ventilating and water-permeating pipe with the diameter of 1.2cm is arranged in the center of the column basin and is as high as the column basin, so that the ventilating and water-permeating of the root system-soil at the middle lower part of the basin is facilitated, and the influence of accumulated water and waterlogging on the growth of the root system is prevented;
3. preparing a substrate, layering and potting, wherein the substrate comprises the following components: sphagnum, edible fungus dregs, vermiculite, perlite, fine sand and the like;
pillar basin matrix layer distribution (the following parts are parts by volume):
a seedling culture layer (5 cm), which is composed of 1 part of sphagnum moss, 1 part of edible fungus residues and 1 part of fine sand, and is favorable for recovering seedlings and culturing strong seedlings;
the root system layer (5 cm), 1 part of vermiculite + 2 parts of coarse sand + 1 part of sphagnum moss + 1 part of edible fungus dregs, which is beneficial to stimulating the generation of lateral roots of salvia miltiorrhiza and increasing the number of roots;
root elongation layer (50 cm): 3 parts of sphagnum moss, 3 parts of edible fungus dregs, 2 parts of vermiculite, 1 part of perlite and 1 part of fine sand and the like, so that the root system can be stretched and pricked, strong seedlings can be used without overgrowth and premature senility, and the strong root system can be obtained.
Firstly, installing a root system elongation layer, and then installing a lateral root meristem on the root system elongation layer; finally, a seedling cultivation layer is arranged on the uppermost layer, compaction is carried out, and water is poured thoroughly;
4. transplanting tissue culture seedlings: transplanting the refined tissue culture seedlings into culture column pots, one for each pot, no. 3 and No. 30 in 2021;
5. water content management: the water content in the bacon substrate is generally controlled to be 70-75%, and the water content in the column pot is gradually reduced from bottom to top along with the downward binding of the root system; after seedling is slowed down, water is sprayed once in 3-5 days generally according to the water change in the column basin, and water accumulation in the basin is prevented strictly;
6. coating moss and removing flowers: in late 6 months in 2021, the salvia miltiorrhiza begin to bolt and flower, and flower buds are timely removed to promote the growth of root systems;
7. adjusting the direction of the column basin: in 2021, 20 days in 9 and 20 days, when the overground part begins to wither, stopping water management, inclining the column basin to the sunny direction by an angle of 30-45 degrees, and completely laying for 1 day in 10 months, so as to promote the redistribution of hormones at different parts in the roots and facilitate the generation of lateral roots or adventitious roots.
Example 2 and field root segment propagation and seed propagation field effect ratio as shown in table 2, it can be seen from table 2 that: the average single root length, the average single plant root length, the number of root producing segments of each plant, the breeding capacity per mu, the number of produced seeds (seedlings) per mu and the breeding coefficient (calculated according to 6000 plants planted per mu) are all obviously improved.
TABLE 2 comparison of field effects of root segment propagation and seed propagation in example 2
The root segment may perform the following operations:
root section seedling culture: cutting into 3-5cm pieces, performing facility germination acceleration and seedling raising (beginning in 2 months of the next year) to generate callus, wherein spores are not expanded.
Transplanting in the field: ridging (ten days in 3 months to 4 months in the next year), planting in proper soil moisture, covering with films, and continuously planting the salvia miltiorrhiza according to a conventional method until harvesting.
Claims (10)
1. A method for culturing the root-segment seedlings or reproducing red sage root includes such steps as quickly reproducing the red sage root, creating the detoxified tissue culture seedlings, culturing the red sage root, culturing the root-segment seedlings and transplanting,
the method for cultivating the salvia miltiorrhiza seeds comprises the following steps:
(1) Making a red sage root cultivating column pot, wherein the height of the pot is 55-65cm, the inner diameter of the pot is 15-20cm, and ventilating and water leaking holes are arranged on the pot wall and the bottom of the pot at positions 7-8cm and 15-16cm away from the bottom of the pot; a ventilating and water-permeating pipe with the diameter of 1.0-1.2cm is arranged in the center of the column basin and is as high as the column basin in height;
(2) Preparing a bacon matrix and filling in layers: the distribution of the matrix layer of the column basin is as follows, and the following parts are calculated according to the volume parts:
the thickness of the seedling culture layer is 5-7cm, and the seedling culture layer comprises the following components: 0.8 to 1.2 parts of sphagnum moss, 0.8 to 1.2 parts of edible fungus residues and 0.8 to 1.2 parts of fine sand;
the thickness of the lateral root meristematic layer is 5-7cm, and the lateral root meristematic layer is composed of: 1.8 to 2.2 parts of vermiculite, 0.8 to 1.2 parts of coarse sand, 0.8 to 1.2 parts of sphagnum moss and 0.8 to 1.2 parts of edible fungus residues;
the thickness of the root system elongation layer is 45-50cm, and the root system elongation layer comprises the following components: 2.5 to 3.5 parts of sphagnum moss, 2.5 to 3.5 parts of edible fungus dregs, 1.8 to 2.2 parts of vermiculite, 0.8 to 1.2 parts of perlite and 0.8 to 1.2 parts of fine sand;
firstly, installing a root system elongation layer, and then installing a lateral root meristem on the root system elongation layer; finally, a seedling cultivation layer is arranged on the uppermost layer, compaction is carried out, and water is poured thoroughly;
(3) Transplanting: transplanting the refined tissue culture seedlings into column pots, one for each pot;
(4) Water regulation and root promotion: the water content in the bacon substrate is controlled to be 70-75%, the water content in the column pot is gradually reduced from bottom to top along with the downward binding of the root system, and water accumulation in the pot is strictly prevented; after seedling buffering, water is sprayed once in 3-5 days generally according to the water change in the column basin, and water accumulation in the basin is prevented strictly;
(5) Removing flowers and increasing roots: removing flower moss after the salvia miltiorrhiza blooms, and promoting the growth of root systems;
(6) Direction-regulating hair roots: when the overground part begins to wither, stopping water management, pouring the column pot in the vertical direction at an angle of 30-45 degrees, placing for 10-20 days to promote redistribution of hormones at different parts in the root so as to be beneficial to generation of lateral roots or adventitious roots, and obtaining 5-7 root strips with the root thickness of 0.6-1.0 cm and the root length of 50-60 cm from the late 10 month to the early 3 months of the next year in each pot.
2. The method as claimed in claim 1, wherein the rapid propagation of Salvia miltiorrhiza Bunge to establish detoxified tissue culture seedling group comprises selecting excellent Salvia miltiorrhiza Bunge, tissue culturing, rapidly propagating, establishing Salvia miltiorrhiza Bunge detoxified tissue culture seedling group, and hardening seedling.
3. The method for cultivating salvia miltiorrhiza bunge in root section seedling culture or propagation as claimed in claim 1, wherein the root section seedling culture comprises the following steps: and (4) cutting the root strips harvested in the step (6) into 3-5cm root segments, and starting facility germination accelerating and seedling raising in 2 months next year to generate callus, wherein the condition that spores are not expanded is met.
4. The method for cultivating salvia miltiorrhiza bunge for root-segment seedling culture or propagation as claimed in claim 1, wherein the field transplantation comprises the following steps: ridging in the middle 3 months to the last 4 months in the next year, planting in proper soil moisture, and conventionally planting after film covering.
5. A cultivation method of Salvia miltiorrhiza Bunge for root section seedling raising or propagation as claimed in any one of claims 1 to 4, wherein two rows of small holes are respectively arranged at positions 7-8cm and 15-16cm away from the bottom of the pot, each row is uniformly distributed with 4 holes around the pot wall, the relative positions of the two rows of holes are arranged in a vertical cross way, and the hole diameters are both 0.4-0.6cm; the bottom of the column basin is uniformly provided with 4 holes with the aperture of 0.8-1.2cm; the bottom end of the middle ventilation and seepage pipe is connected with the bottom of the column basin, and a hole is formed; the pipe wall of the ventilation and seepage pipe is provided with 2 rows of small holes in the longitudinal direction, the longitudinal distance between the holes is 8-10cm, the two rows of small holes are oppositely and crossly arranged, and the aperture is 0.2-0.3cm.
6. A cultivation method of Salvia miltiorrhiza Bunge for root segment seedling or propagation as claimed in any one of claims 1 to 4, wherein the height of the pot is 60cm; a ventilating and water-permeating pipe with the diameter of 1.2cm is arranged in the center of the column basin.
7. A method as claimed in any one of claims 1 to 4, wherein the thickness of the seedling layer is 5cm, the thickness of lateral root meristem is 5cm and the thickness of root elongation is 50cm.
8. A method for cultivating Salvia miltiorrhiza Bunge for root-segment seedling or propagation as claimed in any one of claims 1 to 4, wherein the seedling cultivation layer comprises: 1 part of sphagnum moss, 1 part of edible fungus residues and 1 part of fine sand.
9. A method for cultivating Salvia miltiorrhiza Bunge for root segment seedling or propagation as claimed in any one of claims 1 to 4, wherein the lateral root meristematic layer is composed of: 2 parts of vermiculite, 1 part of coarse sand, 1 part of sphagnum moss and 1 part of edible fungus residue.
10. A cultivation method of Salvia miltiorrhiza Bunge for root segment seedling or propagation as claimed in any one of claims 1 to 4, wherein the root extension layer is: 3 parts of sphagnum moss, 3 parts of edible fungus residues, 2 parts of vermiculite, 1 part of perlite and 1 part of fine sand.
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