CN114615886A - 细胞冷冻保存培养基 - Google Patents

细胞冷冻保存培养基 Download PDF

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CN114615886A
CN114615886A CN202080073778.9A CN202080073778A CN114615886A CN 114615886 A CN114615886 A CN 114615886A CN 202080073778 A CN202080073778 A CN 202080073778A CN 114615886 A CN114615886 A CN 114615886A
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cells
composition
cell
population
car
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K·雷兹瓦尼
刘恩莉
E·施帕尔
R·巴萨尔
D·马林科斯达
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University of Texas System
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    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
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    • A01N1/12—Chemical aspects of preservation
    • A01N1/122—Preservation or perfusion media
    • A01N1/125—Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/12—Chemical aspects of preservation
    • A01N1/122—Preservation or perfusion media
    • A01N1/126—Physiologically active agents, e.g. antioxidants or nutrients
    • A—HUMAN NECESSITIES
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/10—Cellular immunotherapy characterised by the cell type used
    • A61K40/15—Natural-killer [NK] cells; Natural-killer T [NKT] cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/30—Cellular immunotherapy characterised by the recombinant expression of specific molecules in the cells of the immune system
    • A61K40/31—Chimeric antigen receptors [CAR]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
    • A61K40/41—Vertebrate antigens
    • A61K40/42—Cancer antigens
    • A61K40/4202—Receptors, cell surface antigens or cell surface determinants
    • A61K40/421—Immunoglobulin superfamily
    • A61K40/4211—CD19 or B4
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0646—Natural killers cells [NK], NKT cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2239/00—Indexing codes associated with cellular immunotherapy of group A61K40/00
    • A61K2239/38—Indexing codes associated with cellular immunotherapy of group A61K40/00 characterised by the dose, timing or administration schedule
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/50—Cellular immunotherapy characterised by the use of allogeneic cells
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure
    • C12N2500/62—DMSO
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
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    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2315—Interleukin-15 (IL-15)
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/2321—Interleukin-21 (IL-21)

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  • Wood Science & Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
  • Dentistry (AREA)
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  • General Engineering & Computer Science (AREA)
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CN202080073778.9A 2019-08-29 2020-08-25 细胞冷冻保存培养基 Pending CN114615886A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201962893597P 2019-08-29 2019-08-29
US62/893,597 2019-08-29
US202063013823P 2020-04-22 2020-04-22
US63/013,823 2020-04-22
PCT/US2020/047774 WO2021041399A1 (en) 2019-08-29 2020-08-25 Cell cryopreservation medium

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CN114615886A true CN114615886A (zh) 2022-06-10

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US (1) US20220288121A1 (https=)
EP (1) EP4021180A4 (https=)
JP (1) JP2022546486A (https=)
CN (1) CN114615886A (https=)
TW (1) TW202121984A (https=)
WO (1) WO2021041399A1 (https=)

Cited By (3)

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CN114766472A (zh) * 2022-06-06 2022-07-22 吉林省拓华生物科技有限公司 免疫细胞冻存液及其使用方法
CN115885978A (zh) * 2022-12-30 2023-04-04 成都锦欣博悦生物科技有限公司 乳牙间充质干细胞冻存液及其冻存方法
CN117084235A (zh) * 2023-08-16 2023-11-21 上海交通大学医学院 一种外泌体低温冻存保护液及其制备方法

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BR112022014246A2 (pt) 2020-01-21 2022-09-20 Takeda Pharmaceuticals Co Meio de criopreservação para criopreservar células de mamífero, kit, e, métodos de criopreservação de células de mamífero e recuperação de células viáveis
JP7793284B2 (ja) * 2020-03-02 2026-01-05 株式会社ガイアバイオメディシン 高活性ｎｋ細胞の処理方法
MX2023013418A (es) * 2021-05-14 2024-02-28 Gi Cell Inc Composición para criopreservación de células nk y formulación de criopreservación que comprende la misma.
DE102021116694B4 (de) * 2021-06-29 2023-03-09 Promocell Gmbh Wässrige Lösung zur Zellkonservierung
JPWO2023038037A1 (https=) * 2021-09-08 2023-03-16
CN114145287A (zh) * 2021-11-25 2022-03-08 天津尤金生物科技有限公司 一种细胞通用冻存液
CN114271263A (zh) * 2021-12-30 2022-04-05 杭州芯递力生物科技有限公司 一种细胞保存液、冻存套装及细胞冻存方法
CN116458492A (zh) * 2022-01-18 2023-07-21 上海君赛生物科技有限公司 一种细胞冻存组合物及其应用
CN114521549A (zh) * 2022-02-28 2022-05-24 四川中科博瑞生物科技有限公司 一种免疫细胞冻存液和应用及细胞冻存方法和复苏方法
CN117617221A (zh) * 2022-08-11 2024-03-01 南京北恒生物科技有限公司 一种细胞冻存液及其用途
WO2025104700A2 (en) * 2023-11-16 2025-05-22 Takeda Pharmaceutical Company Limited Improved methods for cryopreservation and fitness of natural killer cells, and compositions thereof
KR20250086350A (ko) * 2023-12-06 2025-06-13 (주)나노팬텍 림프 조직 유래 수지상 세포의 냉동 보존 및 배양을 위한 방법 및 조성물
CN117705541B (zh) * 2024-02-05 2024-04-26 江西赛基生物技术有限公司 用于血样pd-1检测的质控品及其制备与质控方法
CN119699307B (zh) * 2024-12-05 2025-10-14 中国农业科学院北京畜牧兽医研究所 蛋白质去乙酰化酶抑制剂nam在家禽精液超低温冷冻保存中的应用
CN119924296B (zh) * 2025-04-08 2025-06-24 济南万泉生物技术有限公司 一种细胞线粒体冷冻保护剂及使用方法

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EP1066833A2 (en) * 1999-07-08 2001-01-10 Humantec Ltd. Cryopreserved cells, system for supplying cells on commission, and preventive or remedial method of infections or cancers
CN103370622A (zh) * 2011-02-14 2013-10-23 埃特根有限公司 使用nk细胞活性的测量值的诊断癌症的方法和诊断试剂盒
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CN108934158A (zh) * 2016-02-01 2018-12-04 Gc细胞治疗 细胞冻存用培养基组合物及其应用
CN105660606A (zh) * 2016-03-10 2016-06-15 广州赛莱拉干细胞科技股份有限公司 一种细胞冻存液
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