CN114605512B - 玉米ppr蛋白或其编码基因在调控植物抗寒性中的应用 - Google Patents

Abstract

本发明涉及涉及基因工程技术领域，尤其涉及玉米PPR蛋白或其编码基因在调控植物抗寒性中的应用。本发明发现玉米PPR蛋白在调控植物抗寒性中存在关键作用，当过表达植物中玉米PPR蛋白的编码基因时，植物的抗寒性能显著提升。本发明提供的PPR家族基因及其编码蛋白为培育耐低温植物新品种提供了资源，同时为研究植物应答低温胁迫的机制和抵御不利环境的分子机理奠定一定的理论基础。

Description

玉米PPR蛋白或其编码基因在调控植物抗寒性中的应用

技术领域

本发明涉及基因工程技术领域，尤其涉及玉米PPR蛋白或其编码基因在调控植物抗寒性中的应用。

背景技术

在自然界中固着生长的植物不可避免遭受各种逆境胁迫的影响，随着水资源的日益匮乏，低温胁迫作为主要的外界环境因子之一，不仅影响植物的生长发育、地理分布，还严重影响作物的产量。因此，研究植物响应干寒胁迫的生理生化变化和分子机理，寻找调控植物响应干寒的关键基因，培育抗寒的作物品种提供有价值的理论支持，以提高农作物的产量是现有技术的研究重点之一。

玉米(Zea mays L)属于禾本科玉蜀黍属植物，是重要的粮食作物和饲料作物，也是全世界总产量最高的农作物。利用转基因技术可以把拟南芥的抗逆基因导入需要改良的玉米遗传物质中，并使其后代表现出稳定遗传的抗逆能力。目前可以通过农杆菌侵染，将过表达质粒导入玉米愈伤组织,再生出具有抗性的转基因植株。选择低拷贝转基因株系进行逆境表型检测，获得具有高效抗逆的转基因玉米，为农业生产提供优良品种资源。

PPR(Pentatricopeptide repeat-containing protein)基因家族是在植物中发现最大的家族之一，它具有35个氨基酸为重复单位连续排列在一起的特征，它可能通过与细胞器转录物的结合，在线粒体或叶绿体中起着重要作用。玉米全基因组共521个PPR编码基因，并在10条染色体上不均匀分布。超过66％的PPR基因很少含有内含子，在同一类/亚类中发现了类似的外显子/内含子结构模式。在60个不同发育阶段检测到的玉米PPR基因的转录丰度出组织特异性表达。

发明内容

为了解决现有技术存在的问题，本发明提供玉米PPR蛋白或其编码基因在调控植物抗寒性中的应用。通过提高植物基因组中玉米PPR蛋白的编码基因的表达水平可以显著提高植物的抗寒性。

第一方面，本发明提供玉米PPR蛋白或其编码基因在调控植物抗寒性中的应用。

本发明进一步提供玉米PPR蛋白或其编码基因在提高植物产量中的应用。

本发明进一步提供玉米PPR蛋白或其编码基因在抗寒植物育种或抗寒转基因植物制备中的应用。

进一步地，所述玉米PPR蛋白具有如下任一种氨基酸序列：

(2)SEQ ID NO.1所示的氨基酸序列经过一个或多个氨基酸的替换、缺失或插入获得的具有相同功能蛋白的氨基酸序列；优选为如SEQ ID NO.5、9、13、17、21或25所示的氨基酸序列。

进一步地，所述玉米PPR蛋白由如下任一种核苷酸序列编码得到：

(1)SEQ ID NO.2所示的核苷酸序列；

(2)SEQ ID NO.2所示的核苷酸序列经过一个或多个核苷酸的替换、缺失或插入获得的具有相同功能蛋白的编码核苷酸序列；优选如SEQ ID NO.6、10、14、18、22或26所示的核苷酸序列。

进一步地，所述植物为单子叶植物或双子叶植物，优选为玉米。

第二方面，本发明提供一种调控植物抗寒性的方法，包括：

调控植物中玉米PPR蛋白的表达水平，所述PPR蛋白具有如下任一种氨基酸序列：

(1)SEQ ID NO.1所示的氨基酸序列；

(2)SEQ ID NO.1所示的氨基酸序列经过一个或多个氨基酸的替换、缺失或插入获得的具有相同功能蛋白的氨基酸序列；优选为如SEQ ID NO.5、9、13、17、21或25所示的氨基酸序列。

进一步地，通过提高所述植物中所述玉米PPR蛋白的编码基因的表达水平，提高所述植物的抗寒能力；或，通过将所述玉米PPR蛋白的编码基因过表达的株系与其他株系杂交，培育抗寒株系。

进一步地，提高所述植物中所述玉米PPR蛋白的编码基因的表达水平，具体为：

将包含所述玉米PPR蛋白的编码基因的载体转化至农杆菌EHA105菌株中，再浸染玉米愈伤组织，得到转基因植株的幼苗。

本发明进一步提供包含所述玉米PPR蛋白的编码基因的生物材料，所述生物材料为表达盒、载体或转基因细胞。

作为一种优选的实施方式，本发明提供一种构建抗寒的转基因玉米的方法，具体包括如下流程：

1)提取玉米总RNA，反转录获得cDNA，以cDNA为模板，F和R为引物，扩增PPR基因，将扩增产物构建到表达载体为含有Ubi启动子的pBCXUN载体。获得的重组表达载体命名为pBCXUN-PPR；

2)用pBCXUN-PPR转化农杆菌EHA105，然后利用转化的农杆菌侵染玉米愈伤组织，获得抗低温的转基因玉米幼苗。

其中，步骤1)中所述引物F和R的核苷酸序列如Seq ID No.3和4所示，所述玉米优选为LH244，即具有纯合基因型的玉米植株。

将本发明的PPR基因过表达后，玉米表现为抗低温的表型。为了便于对转基因植物进行鉴定及筛选，可对所使用的载体进行加工，如加入植物可选择性标记或具有抗性的抗生素标记物等。

本发明具备如下有益效果：

本发明通过对编码植物内响应调节子的基因PPR的研究，发现过表达该基因的转基因植株较野生型植株具有明显抗低温的表型。本发明提供的PPR基因为培育抗低温植物新品种提供了基因资源，为研究植物应答逆境信号的机制和耐受不利环境的分子机理奠定理论基础。

附图说明

图1为本发明实施例1中过表达株系的PPR基因过量表达图。

图2为本发明实施例2中过表达株系低温处理前和低温处理恢复后植株生长情况照片。

图3为本发明实施例3中过表达株系离子渗漏率统计图。

图4为本发明实施例3中PPR的同源基因的real time数据结果。

具体实施方式

以下实施例用于说明本发明，但不用来限制本发明的范围。

若未特别指明，实施例均按照常规实验条件，如Sambrook等分子克隆实验手册(Sambrook J&Russell DW,Molecular cloning:a laboratory manual,21)，或按照制造厂商说明书建议的条件。

以下实施例中的主要试剂为：各种限制性内切酶、Taq DNA聚合酶、T4连接酶、Pyrobest Taq酶、KOD购自NEB、Toyobo等生物公司；dNTPs购自Genestar公司；质粒小提试剂盒和琼脂糖凝胶回收试剂盒购自上海捷瑞生物工程公司；琼脂粉、琼脂糖、氨苄青霉素(Amp)、卡那霉素(Kan)、硫酸庆大霉素(Gen)、利福平(Rif)等抗生素以及Glucose、BSA、、LBMedium等购自Sigma、Bio-Rad等公司；实时定量PCR所用的试剂购自于TaKaRa，实施例中所使用的各种其它化学试剂均为进口或国产分析纯试剂。

实施例中所使用的引物由六合华大公司合成，并进行相关测序。

实施例1PPR基因过表达载体的构建和检测

从B73玉米(Zea mays L.)提取总RNA，反转录获得cDNA，以cDNA为模板，F和R为引物，扩增PPR基因，引物带有酶切位点，酶切后连到过表达载体上。PPR基因过表达载体构建方法如下：

(1)用Magen公司的RNA提取试剂盒提取B73玉米总RNA，具体步骤参照试剂盒说明书。

(2)用thermo公司的反转录试剂盒将RNA反转录出cDNA，具体步骤参照试剂盒说明书。

(3)以cDNA为模板，F和R为引物，扩增PPR基因的cDNA(如SEQ ID NO.2所示，其编码氨基酸序列如SEQ ID NO.1所示)，将扩增产物跑电泳切胶回收，回收方法参照天根公司试剂盒。

所用扩增PPR基因cDNA的引物为：

上游引物F：5’-ATGCATCCGCCTTCCCGCCT-3’(Seq ID No.3)

下游引物R：5’-CTAAATATGTGAGCCACTTT-3’(Seq ID No.4)

(4)将回收的PPR基因cDNA和pBCXUN载体用Xba I和Cla I双酶切，酶切产物跑电泳切胶回收。回收产物用T4连接酶连接。将PPR基因连接至pBCXUN载体(pBCXUN载体以商业化载体pCAMBIA1300为骨架，将其中的潮霉素抗性基因hpt替换为除草剂抗性基因barM；同时将玉米泛素基因Ubi的启动子通过酶切连接的方式克隆到载体上，驱动下游过表达基因的转录)，以Ubi启动子驱动PPR基因的表达。

(5)取5μL酶切-连接体系的产物，转化大肠杆菌感受态。在含有50μg/mL卡那霉素的LB平板上筛选。菌落PCR鉴定单克隆，挑选阳性克隆测序。获得的测序正确的重组表达载体命名为pBCXUN-PPR。将上一步所得的质粒酶切后，进行电泳检测，具体的方法为：用Xba I和Cla I酶切UBI:PPR，用1％琼脂糖凝胶，120V，50mA电泳后，UVP Gel Documentation凝胶分析系统扫描成像。

实施例2PPR基因过表达植物的构建和检测

将实施例1所述含有PPR基因的pBCXUN载体转化到农杆菌EHA105菌株中，再侵染玉米愈伤组织，得到转基因幼苗。具体方法为：将含有目的载体的农杆菌接种于100mL LB三抗液体培养液(Kan 50μg/mL，Rif 50μg/mL，Gen 50μg/mL)中，28℃振荡培养过夜，待OD₆₀₀值为1.0-2.0，以5000g，室温离心15min，收集菌体；用2mL转化液(1/2MS，5％蔗糖，40μL SilwetL-77)悬浮菌体；将玉米愈伤组织浸泡在农杆菌的转化液中，封口。放回光照培养架上正常生长至长出植株。然后进行筛选得到的种子进行低温逆境处理实验。

本实施例中分离得到过表达株系OE-1和OE-4，采用实时定量PCR检测所得过表达株OE-1和OE-4中PPR基因的表达，具体步骤如下：

1)提取植物总RNA，并反转录获得cDNA。

2)将反转录得到的cDNA稀释5倍后，用Takara试剂盒进行实时定量PCR，所用反应体系包括：2×SYBR Premix ExTaq buffer，0.2μL DyII，0.4μLPrimer(F/R)，2μL cDNA模板，最后用ddH₂O补齐至20μL，充分混匀后放入ABI PRISM 75实时定量PCR仪中进行两步法进行PCR扩增，反应条件为：95℃30s；95℃5s；60℃40s；40cycles。

实时定量PCR反应完成后根据2^-ΔΔCt的原理计算出野生型和过表达之间相对表达量并作图分析。在扩增所鉴定基因的同时，每个样品以UBI作为内参同时扩增。

实施例3过表达PPR基因植物的抗低温能力检测

首先将CK(未转基因的对照)、OE-1和OE-4的种子播种在黑土，进口土和蛭石(1:1:1)的长10cm，宽10cm，高10cm的小盆里，每个盆里放5粒，再覆2cm土放置于托盘中，浇水至土完全湿润，放置于23度的培养室中，16h光照，8小时黑暗。生长14天后进行4度低温处理直到第二叶皱缩萎蔫后，拿出来放到23度培养室恢复两天后拍照以及取材进行离子渗漏率的统计。

本实施例中离子渗漏率的统计采用测叶片的相对电导率L＝(S1-S0)/(S2-S0)。将低温处理后玉米的一整棵植株全部放到装有10ml蒸馏水的15ml离心管中，用真空泵抽气30min,然后置于摇床室温摇1h,之后用电导仪测其初电导值为S1，然后再将样品置于沸水中水浴15min,取出置于摇床摇2h，再测电导率记为S2。S0为空白对照蒸馏水的电导率。

结果显示(图2、图3)，OE-1和OE-4均表现出抗低温的表型。过表达PPR基因能够使得玉米抗冻能力增强。

除此之外，本发明在研究过程中在玉米RNA-seq数据分析过程中进一步发现PPR基因的多个同源基因，其表达水平均受到低温胁迫(如图4)，其中GRMZM2G047255的氨基酸序列为SEQ ID NO.5，核苷酸序列为SEQ ID NO.6，用于扩增的引物序列为SEQ ID NO.7-8；GRMZM2G421690的氨基酸序列为SEQ ID NO.9，核苷酸序列为SEQ ID NO.10，用于扩增的引物序列为SEQ ID NO.11-12；GRMZM2G121579的氨基酸序列为SEQ ID NO.13，核苷酸序列为SEQ ID NO.14，用于扩增的引物序列为SEQ ID NO.15-16；GRMZM2G070381的氨基酸序列为SEQ ID NO.17，核苷酸序列为SEQ ID NO.18，用于扩增的引物序列为SEQ ID NO.19-20；GRMZM2G312954的氨基酸序列为SEQ ID NO.21，核苷酸序列为SEQ ID NO.22，用于扩增的引物序列为SEQ ID NO.23-24；GRMZM2G046919的氨基酸序列为SEQ ID NO.25，核苷酸序列为SEQ ID NO.26，用于扩增的引物序列为SEQ ID NO.27-28。

虽然，上文中已经用一般性说明及具体实施方案对本发明作了详尽的描述，但在本发明基础上，可以对之作一些修改或改进，这对本领域技术人员而言是显而易见的。因此，在不偏离本发明精神的基础上所做的这些修改或改进，均属于本发明要求保护的范围。

序列表

<110> 中国农业大学

<120> 玉米PPR蛋白或其编码基因在调控植物抗寒性中的应用

<130> KHP201118201.6

<160> 28

<170> SIPOSequenceListing 1.0

<210> 1

<211> 648

<212> PRT

<213> 玉米(Zea mays)

<400> 1

Met His Pro Pro Ser Arg Leu Leu Val Leu Leu His Ala Gly Ala Val

1 5 10 15

His Pro Pro Ser Phe Arg Val Leu Leu Lys Leu His Ala His His Leu

20 25 30

Val Ser Gly Leu Leu Ser Ser His Ser Thr Ser Pro Phe Ile Asp Arg

35 40 45

Leu Val Ala Ala Phe Ala His Ser Asp Pro Ala Ser Pro Arg Pro Leu

50 55 60

Leu His Ala Leu Ala Ile Leu Ala Ser Leu Pro Ser Pro Pro Asp Ser

65 70 75 80

Ala Phe Pro Tyr Asn Ala Ala Phe Arg Ala Leu Ser Leu Cys Pro His

85 90 95

Gln His Leu Val Asp Arg His Cys Leu Pro Leu Tyr Arg Ala Leu Leu

100 105 110

His Ser Gly Ser Ala Arg Pro Asp His Leu Thr Phe Pro Phe Leu Ile

115 120 125

Lys Ala Cys Ala Arg Leu Gln Tyr Arg Ser Tyr Gly Ala Ala Val Leu

130 135 140

Gly His Val Gln Lys Leu Gly Phe Ser Ala Asp Val Phe Val Val Asn

145 150 155 160

Ala Ala Met His Phe Trp Ser Val Arg Gly Pro Met Ala Phe Ala Arg

165 170 175

Arg Leu Phe Asp Glu Ser Pro Val Arg Asp Val Val Ser Trp Asn Thr

180 185 190

Leu Ile Gly Gly Tyr Val Arg Ser Gly Leu Pro Arg Glu Ala Leu Glu

195 200 205

Leu Phe Trp Arg Leu Ala Glu Asp Gly Asn Ala Val Arg Pro Asp Glu

210 215 220

Val Thr Val Ile Gly Ala Val Ser Gly Cys Ala Gln Met Gly Asp Leu

225 230 235 240

Glu Leu Gly Lys Arg Leu His Glu Phe Val Asp Asn Lys Gly Val Arg

245 250 255

Cys Thr Val Arg Leu Met Asn Ala Val Met Asp Met Tyr Val Lys Cys

260 265 270

Gly Ser Leu Glu Leu Ala Asn Ser Val Phe Glu Arg Ile Ser Asn Arg

275 280 285

Thr Ala Val Ser Trp Thr Thr Met Ile Val Gly His Ala Arg Leu Gly

290 295 300

Met Met Glu Asp Ala Arg Met Leu Phe Asp Glu Met Pro Glu Arg Asp

305 310 315 320

Val Phe Pro Trp Asn Ala Leu Met Ala Gly Tyr Val Gln Asn Lys Gln

325 330 335

Gly Lys Glu Ala Ile Ala Leu Phe His Glu Met Gln Lys Ser Lys Val

340 345 350

Asp Pro Asn Glu Ile Thr Met Val Asn Leu Leu Ser Ala Cys Ser Gln

355 360 365

Leu Gly Ala Leu Glu Met Gly Met Trp Val His His Tyr Ile Asp Arg

370 375 380

His Lys Leu His Leu Ser Val Ala Leu Gly Thr Ser Leu Val Asp Met

385 390 395 400

Tyr Ala Lys Cys Gly Asn Ile Lys Lys Ala Ile Cys Val Phe Asn Glu

405 410 415

Ile Pro Val Gln Asn Ala Leu Thr Trp Thr Ser Met Ile Cys Gly Leu

420 425 430

Ala Asn His Gly His Ala Asp Glu Ala Ile Glu Tyr Phe Gln Arg Met

435 440 445

Ile Asp Leu Gly Leu Gln Pro Asp Glu Ile Thr Phe Ile Gly Val Leu

450 455 460

Ser Ala Cys Cys His Ala Gly Leu Val Glu Ala Gly Arg Gln Phe Phe

465 470 475 480

Ser Leu Met His Ala Lys Tyr His Leu Glu Arg Lys Met Lys His Tyr

485 490 495

Ser Cys Met Ile Asp Leu Leu Gly Arg Ala Gly His Leu Asp Glu Ala

500 505 510

Glu Gln Leu Val Asn Ala Met Pro Met Asp Pro Asp Ala Val Val Trp

515 520 525

Gly Ala Leu Phe Phe Ala Cys Arg Met His Gly Asn Ile Thr Leu Gly

530 535 540

Glu Lys Ala Ala Met Lys Leu Val Glu Leu Asp Pro Ser Asp Ser Gly

545 550 555 560

Ile Tyr Val Leu Leu Ala Asn Met Tyr Ala Glu Ala Asn Met Arg Lys

565 570 575

Lys Ala Asp Lys Val Arg Val Met Met Arg His Leu Gly Val Glu Lys

580 585 590

Val Pro Gly Cys Ser Cys Ile Glu Leu Asn Gly Val Val His Glu Phe

595 600 605

Ile Val Lys Asp Lys Ser His Leu Asp Thr Asn Ala Ile Tyr Asp Cys

610 615 620

Leu His Glu Ile Thr Leu Gln Met Arg His Ile Ala Asn Leu Ile Asp

625 630 635 640

Ile Ser Glu Ser Gly Ser His Ile

645

<210> 2

<211> 1947

<212> DNA

<213> 玉米(Zea mays)

<400> 2

atgcatccgc cttcccgcct gctggtcctc ctccacgccg gcgccgtcca cccgccgtcg 60

ttccgcgtcc ttctgaagct tcacgcgcac cacctcgtct ccggcctcct ctcctcccac 120

tccacgtcgc ccttcatcga ccgcctcgtc gccgcctttg cccactccga ccccgcctct 180

ccgcgcccgc ttctccacgc gcttgccatc ctcgcttccc tcccatcccc gccggactct 240

gcattcccct acaatgccgc cttccgcgcg ctctccctct gcccgcatca acacctcgtg 300

gaccgccact gcctcccgct ctaccgcgcc ctcctccact ccggttccgc gcgccccgat 360

cacctcacgt tcccgttcct catcaaggcc tgtgcgcgcc tgcagtacag atcgtacggt 420

gccgcggttc ttgggcatgt gcagaaactg gggttcagcg ctgacgtctt cgtggtgaac 480

gccgcgatgc atttctggtc tgttcgtggg cccatggctt tcgcccgaag gttgttcgac 540

gaaagtccag tcagggacgt ggtctcgtgg aacacactga ttggtgggta cgtgaggagt 600

ggcctcccgc gggaggcgct agagttgttt tggaggttgg cggaagacgg caatgcagtg 660

aggcctgacg aggtcacggt gatcggagca gtgtcgggat gtgcacagat gggggatttg 720

gagctaggga agaggctgca tgagtttgtg gacaataaag gggtgaggtg cacggtgagg 780

ctgatgaacg cggtgatgga tatgtatgtc aagtgtggca gtttagagtt agcaaactcg 840

gtgtttgaaa ggatcagtaa cagaactgct gtttcttgga caaccatgat cgtggggcat 900

gcgaggctgg ggatgatgga agatgcacgg atgttgttcg atgagatgcc tgaaagggat 960

gtattcccat ggaatgctct gatggctggt tatgtacaga ataagcaggg caaggaggct 1020

attgcactgt ttcatgagat gcagaaatca aaggtggatc ctaatgagat caccatggtt 1080

aatctcttat ctgcttgctc gcagctagga gcattagaaa tggggatgtg ggtccaccat 1140

tacattgacc gacataaact tcatcttagt gttgcacttg gtacatctct ggtggacatg 1200

tatgcaaagt gtggcaacat caagaaggca atctgtgttt tcaatgaaat cccagttcaa 1260

aatgcactca catggacctc aatgatatgt ggtcttgcaa atcatggaca tgctgatgag 1320

gccatagaat acttccagag aatgatagat cttggattgc agccagatga gataacattt 1380

ataggggttc tgtcagcttg ttgccatgcc ggtttggttg aagcaggtcg tcaatttttc 1440

tccttaatgc atgcaaaata tcaccttgag aggaaaatga aacattattc atgtatgata 1500

gacttgctag gcagagcagg gcatttggat gaagcagaac agcttgtgaa cgccatgcct 1560

atggatcctg atgcagtagt ttggggtgct cttttctttg cttgtaggat gcatggtaat 1620

ataactcttg gtgagaaggc agcaatgaaa ttggtagaac ttgacccaag tgatagtgga 1680

atctatgtgt tattggctaa catgtatgcg gaggcaaata tgaggaagaa ggctgacaaa 1740

gtgagggtta tgatgaggca tttgggagtg gagaaggttc ctggatgtag ttgcattgag 1800

ctaaacggtg ttgttcatga atttatcgtg aaagataagt ctcatttgga cactaatgca 1860

atttatgact gcttacatga gatcacatta caaatgaggc atattgcaaa cctgattgac 1920

atttccgaaa gtggctcaca tatttag 1947

<210> 3

<211> 20

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 3

atgcatccgc cttcccgcct 20

<210> 4

<211> 20

<212> DNA

<213> 人工序列(Artificial Sequence)

<400> 4

ctaaatatgt gagccacttt 20

<210> 5

<211> 639

<212> PRT

<213> 玉米(Zea mays)

<400> 5

Met Val Thr Gly Arg Val Lys Ala Ala Met Gly Phe Gln Arg Ser Pro

1 5 10 15

Lys Ser Gln Val Pro Glu Ala Ala Ser Pro Ser Arg Ala Pro Thr Ser

20 25 30

Gly Ser Val Ser Thr Ala Gly Ser Ala Pro Ser Lys Ala Ser Ala Leu

35 40 45

Ala Arg Ser Phe Gly Ala Tyr Phe Pro Arg Ser Ser Ala Gln Val Arg

50 55 60

Pro Ala Ala Arg Ala Pro Pro Gln Val Ala Glu Leu Leu Arg Ala Ile

65 70 75 80

Glu Gln Leu Gln Glu Arg Glu Ser Arg Leu Arg Val Glu Leu Leu Glu

85 90 95

Gln Lys Ile Leu Lys Glu Thr Val Ala Ile Val Pro Phe Leu Glu Ala

100 105 110

Glu Leu Ala Ala Lys Lys Ala Glu Leu Gln Arg Cys Arg Asp Thr Ala

115 120 125

Glu Arg Leu Glu Ala Glu Asn Gly Arg Leu Cys Ala Glu Leu Asp Ala

130 135 140

Ala Ala Leu Glu Val Thr Ser Arg Lys Gln Arg Ile Val Glu Leu Glu

145 150 155 160

Lys Glu Met Ala Glu Leu Arg Thr Arg Gln Asp Ala Ala Ala Asp Ala

165 170 175

Asp Asp Cys Ser Ser Ser Ala Ser Ala Pro Ser Glu Arg Leu Glu Ser

180 185 190

Ser Ser Ala Ala Pro Lys Pro Asn Arg Ala Ser Leu Ala Gln Leu Asp

195 200 205

Pro Pro Ala Pro Pro Ala Pro Gly Pro Ala Pro Leu Lys Ser Lys Ser

210 215 220

Tyr Phe Ser Ala Ser Ser Ser His Pro Ser Pro Ala Ser Ser Ser Pro

225 230 235 240

Pro Pro Ser Pro Ser Ser Ser Thr Ser Thr Ser Ser Ser Pro Ala His

245 250 255

Ser Arg Ser Ser Ser Asp Thr Ala Ala Ala Pro Arg Pro Arg Val Val

260 265 270

Glu Leu Ser Lys Leu Pro Pro Ile Pro Pro Pro Pro Pro Pro Cys Pro

275 280 285

Pro Pro Pro Pro Pro Ser Arg Ser Lys Arg Ser Ser Pro Pro Asn Ser

290 295 300

Ala Ser Val Asp Gly Ser Ala Ala Ala Ala Pro Pro Pro Pro Pro Pro

305 310 315 320

Pro Pro Pro Pro Pro Pro Pro Pro Ala Arg Arg Gln Phe Gly Ala Ala

325 330 335

Pro Ala Pro Pro Ala Gly Ala Ser Ser Gly Gln Gly Asp Val Arg Arg

340 345 350

Val Pro Glu Val Val Glu Phe Tyr His Ser Leu Met Arg Arg Glu Ser

355 360 365

Lys Arg Asp Gly Ser Gly Thr Ala Ser Glu Ala Ala Asn Gly Gly Gly

370 375 380

Gly Gly Ala Ala Ala Thr Arg Asp Met Ile Gly Glu Ile Glu Asn Arg

385 390 395 400

Ser Ala His Leu Leu Ala Ile Lys Ser Asp Val Glu Arg Gln Gly Asp

405 410 415

Phe Ile Arg Phe Leu Ile Lys Glu Val Glu Gly Ala Ala Phe Val Asp

420 425 430

Ile Glu Asp Val Val Ser Phe Val Lys Trp Leu Asp Asp Glu Leu Ser

435 440 445

Arg Leu Val Asp Glu Arg Ala Val Leu Lys His Phe Glu Trp Pro Glu

450 455 460

Asn Lys Ala Asp Ala Leu Arg Glu Ala Ala Phe Gly Tyr Cys Asp Leu

465 470 475 480

Lys Lys Leu Glu Arg Glu Ala Ala Ser Phe Arg Asp Asp Ala Arg Gln

485 490 495

Pro Cys Ala Ala Ala Leu Lys Lys Met Gln Ala Leu Phe Glu Lys Leu

500 505 510

Glu His Gly Val Tyr Asn Leu Ala Arg Val Arg Asp Ala Ala Thr Gly

515 520 525

Arg Tyr Thr Arg Phe Gln Ile Pro Trp Glu Trp Met Lys Gln Asp Thr

530 535 540

Gly Ile Val Ser Gln Ile Lys Leu Gln Ser Val Lys Leu Ala Met Lys

545 550 555 560

His Leu Lys Arg Val Ser Ser Glu Leu Glu Val Ile Lys Gly Gly Pro

565 570 575

Glu Glu Glu Glu Gln Glu Leu Met Leu Gln Gly Val Arg Phe Ala Phe

580 585 590

Arg Val His Gln Phe Ala Gly Gly Phe Asp Val Asp Thr Met Arg Ala

595 600 605

Phe Gln Glu Leu Lys Glu Lys Ala Ser Met Cys Arg Val Gln Arg Gln

610 615 620

Lys Gln His Arg His Leu Arg Gln His Arg Leu Val Ala Arg Ala

625 630 635

<210> 6

<211> 1920

<212> DNA

<213> 玉米(Zea mays)

<400> 6

atggtgacag gccgggtcaa ggcggccatg ggcttccagc gcagccccaa gtcgcaggtg 60

ccggaggcgg cgtctccttc tcgggcgcct acgtcggggt ccgtgtccac cgccgggtcg 120

gcgccgtcca aggcgtcggc gctcgcgcgg tccttcggcg cgtacttccc gcgctcttcg 180

gcgcaggtgc gccccgccgc ccgcgcgccg ccgcaggtcg ccgagctgct gcgcgccatc 240

gagcagctcc aggagcggga gtcgcgcctc cgcgtggagc tgctggagca gaagatactc 300

aaggagaccg tcgccatcgt cccgttcctg gaggccgagc tcgcggccaa gaaggccgag 360

ctccagaggt gcagggacac cgcggagcgg ctggaggccg agaacgggcg gctgtgcgcc 420

gagctcgacg ccgccgcgct cgaggtcacg agcaggaagc agaggatcgt cgagctggag 480

aaggagatgg cggagctgag gacgcggcag gacgcggcag cggacgccga cgactgctcc 540

tcgtccgctt cggcgccgag cgagcgtctc gagagctcca gcgctgcgcc aaagccaaac 600

cgcgcgagtt tagctcagct tgatcctcca gcgccgccag cgccagggcc agcgcctttg 660

aagtccaagt cctacttctc cgcgagctcg tcgcaccctt cgcccgcaag ctcgtcgccg 720

ccgccctcgc cgtcttcctc cacgtcgacc tcgtcttcgc cagcgcactc ccgctcgtcg 780

tcggacacgg cagccgctcc aaggccccgc gtggtagagc tctcaaagct cccgcccata 840

cctccgccgc cgccgccttg ccctcctccg ccacctccca gcaggagcaa gagatcctct 900

cccccgaact cagccagcgt ggacggaagc gcagccgccg ctccgcctcc cccgccgccg 960

ccgccgccgc cgcctccccc tccggcaagg aggcagttcg gcgccgcgcc agcaccgcct 1020

gcaggggcat cgtcggggca gggcgacgtg aggcgcgtgc ccgaggtggt ggagttctac 1080

cactcgttaa tgcggcggga gtccaagagg gacggcagcg gcacagcaag tgaggccgcg 1140

aacggaggcg gcggcggcgc ggcggcgacg cgcgacatga tcggagagat cgagaaccgg 1200

tctgcgcatc tcctcgcgat caagtcggac gtggagaggc agggcgactt catccggttc 1260

ctgatcaagg aggtggaggg cgcggcgttc gtggacatcg aggacgtcgt gagcttcgtc 1320

aagtggctgg acgacgagct ctcgcgcctg gtggacgagc gcgcggtgct gaagcacttc 1380

gagtggccgg agaacaaggc ggacgcgctc cgcgaggcgg cgttcggcta ctgcgacctc 1440

aagaagctgg agcgggaggc ggcctccttc cgcgacgacg cgcgccagcc ctgcgccgcg 1500

gcgctcaaga agatgcaggc gctcttcgag aagctggagc acggcgtgta taacctcgcc 1560

cgcgtccggg acgccgccac cggccgctac acccggttcc agatcccgtg ggagtggatg 1620

aagcaggaca ccggcatcgt cagccagatc aaactccagt cggtgaagct ggcaatgaag 1680

cacctgaaac gggtttcctc cgagctcgag gtgatcaagg gaggccctga agaggaggag 1740

caggagctga tgctccaagg agtacggttc gccttcaggg tacaccagtt cgcaggtggg 1800

ttcgacgtgg acaccatgcg agccttccag gagctcaagg agaaggcgtc catgtgccgg 1860

gtacagcggc agaagcagca ccgtcacctg cgccagcaca ggctcgttgc cagggcctga 1920

<210> 7

<211> 20

<212> DNA

<213> 玉米(Zea mays)

<400> 7

agtcctactt ctccgcgagc 20

<210> 8

<211> 20

<212> DNA

<213> 玉米(Zea mays)

<400> 8

gtatgggcgg gagctttgag 20

<210> 9

<211> 535

<212> PRT

<213> 玉米(Zea mays)

<400> 9

Met Pro Pro Pro Leu Leu Glu Phe Ala Ile Leu Ala Thr Ala Cys Arg

1 5 10 15

Phe Ser Lys Pro Leu Thr Val Leu Arg Arg Leu Gly Val Leu Gly Pro

20 25 30

Pro Thr Val Arg Arg Asn Ala Ala Glu Ala Phe Val Pro Ser Pro Phe

35 40 45

Arg Gln Val Val Val Gln Ser Gly Lys Leu Leu Gly Thr Pro Arg Phe

50 55 60

Cys His Thr Thr Ala Gly Met Ser Glu Cys Leu Pro Asp Val Tyr Ala

65 70 75 80

Ser Gln Val Leu Lys Val Leu Lys Ser Thr Ser Ala Asp Asn Thr Asp

85 90 95

Leu Ser Gly Ala Leu His Gln Phe Ala Val His Met Asp Glu Asp Ile

100 105 110

Ile Leu Met Val Leu Gln Lys Gln Arg Ser Asn Trp Gln Val Ala Leu

115 120 125

Ala Phe Phe Asn Trp Ala Ala Thr Leu Pro Gly Tyr Ala His Gly Ser

130 135 140

Arg Ala Tyr Thr Glu Met Leu Asp Ile Leu Gly Arg Met Lys Lys Val

145 150 155 160

Arg His Met Arg Gln Leu Phe Asp Glu Ile Pro Glu Glu Arg Arg Gln

165 170 175

Val Val Val Thr Asn Lys Met Phe Ala Val Leu Leu Asn Arg Tyr Ala

180 185 190

Gly Ala His Lys Val Gln Glu Ala Ile Glu Val Phe Tyr Leu Arg Lys

195 200 205

Asp Tyr Gly Phe Glu Leu Asp Leu Val Gly Phe Gln Ile Leu Leu Met

210 215 220

Ser Leu Cys Arg Tyr Lys His Val Glu Glu Ala Glu Ala Leu Phe Arg

225 230 235 240

Glu Lys Lys Asp Glu Phe Pro His Val Thr Lys Ser Trp Asn Ile Ile

245 250 255

Leu Asn Gly Trp Cys Val Lys Gly Ser Leu Arg Asp Ala Gln Arg Ile

260 265 270

Trp Asn Asp Ile Ile Ala Ser Lys Val Glu Arg Asp Leu Phe Thr Phe

275 280 285

Gly Thr Phe Ile Asn Ala Leu Ala Lys Asn Gly Arg Ile Ser Ala Ala

290 295 300

Val Lys Leu Phe Lys Gln Met Trp Glu Lys Gly Ile Asn Pro Asp Val

305 310 315 320

Ala Ile Cys Asn Cys Ile Ile Asp Gln Leu Cys Phe Lys Lys Lys Ile

325 330 335

Pro Glu Ala Leu Glu Ile Phe Gly Glu Met Asn Asp Arg Leu Cys Gln

340 345 350

Ala Asp Val Ala Thr Tyr Asn Thr Leu Ile Lys Tyr Met Cys Lys Ile

355 360 365

Lys Arg Met Glu Lys Val Tyr Glu Leu Leu Asp Asp Met Glu Ala Lys

370 375 380

Gly Cys Pro Pro Asn Asn Arg Thr Tyr Ser Tyr Ile Leu Lys Thr Thr

385 390 395 400

Glu Lys Pro Lys Asp Val Ile Ala Leu Met Gln Arg Met Glu Arg Thr

405 410 415

Gly Cys Lys Leu Asp Ser Asp Ile Tyr Asn Leu Ile Leu Asn Leu Tyr

420 425 430

Ile Lys Trp Lys Tyr Glu Lys Gly Met Gln Gln Ile Trp Asp Glu Met

435 440 445

Glu Arg Arg Gly Ser Gly Pro Asp Gln Arg Ser Phe Thr Ile Met Val

450 455 460

His Gly Leu His Ser Gln Gly Lys Leu Asp Glu Ala Leu Gln Tyr Tyr

465 470 475 480

Thr Thr Met Lys Ser Arg Gly Met Ile Pro Glu Pro Arg Thr Lys Ile

485 490 495

Leu Val Lys Ala Met His Met Lys Lys Asp Gly Ala Ala Thr Glu Asp

500 505 510

Asp Ser Ala Ser Met Ala Gly Lys His Leu Lys Leu Asp His Arg Ser

515 520 525

Arg Leu Phe Asn Val His Arg

530 535

<210> 10

<211> 1608

<212> DNA

<213> 玉米(Zea mays)

<400> 10

atgccgccgc cgctgcttga gttcgcgatc ctcgccactg cctgcaggtt cagcaagccc 60

cttaccgttc tcaggaggct tggcgttctt ggcccaccaa ccgttcgacg caatgccgca 120

gaggcgttcg tgccttcacc gtttcgccag gttgtcgttc agagtggaaa gcttcttggg 180

acaccaaggt tctgccacac cactgccggc atgtcagaat gtctcccgga cgtctacgcc 240

agtcaggtac tgaaggttct caagtccacc agtgctgaca acacggatct cagcggtgct 300

cttcatcagt tcgcggtcca catggatgag gatattatcc tcatggtcct ccagaagcag 360

cggtccaact ggcaggtcgc cctggcattc ttcaactggg cggcaaccct gccggggtat 420

gcccacgggt caagggccta caccgagatg ctggacattc ttgggcggat gaagaaggtc 480

agacacatga ggcagctgtt tgacgagatt cctgaggagc gacgtcaggt ggtcgtgacg 540

aataagatgt ttgccgtgct gttgaaccgg tacgctggtg cgcacaaggt gcaggaggcg 600

atagaggtgt tctacttgag gaaggattac gggtttgagc ttgacttggt cgggttccag 660

atactactga tgtcgctgtg caggtataag catgtcgagg aggcggaggc gctgttccgt 720

gagaagaaag atgagttccc gcatgtcaca aagagctgga acattatact aaatggttgg 780

tgtgtcaagg gaagcctgag ggacgctcaa aggatttgga atgatatcat tgcatctaag 840

gttgaacgag acctattcac ctttggtacg ttcataaacg cattggccaa aaatggcaga 900

attagtgccg cggtgaagct gttcaaacag atgtgggaga aagggatcaa tcctgatgtt 960

gcgatttgca attgtatcat cgaccagctg tgcttcaaga aaaagattcc agaagcactt 1020

gagatttttg gtgagatgaa tgaccggctt tgtcaagccg atgtggctac ctacaacact 1080

ttgataaaat acatgtgtaa gataaaaagg atggagaaag tttatgaact tttggatgac 1140

atggaagcaa agggctgccc tccaaacaat aggacatact cctacattct gaagacgact 1200

gagaaaccca aagatgtcat tgccttgatg cagaggatgg agcgaaccgg ctgcaaattg 1260

gattctgata tatacaactt gatattgaac ctttatatca aatggaaata tgagaagggg 1320

atgcaacaga tatgggatga gatggagaga agagggtcag gcccagatca gcggtcgttt 1380

actattatgg ttcatggtct tcattcccaa ggcaagctcg atgaggctct gcagtattac 1440

acaacaatga agtctagagg gatgattcca gaacctagga ccaagatact ggtgaaagct 1500

atgcacatga agaaggacgg ggctgccact gaagatgact cggcaagtat ggcagggaag 1560

catctaaaat tggatcatcg ttcaagacta ttcaatgttc atagatag 1608

<210> 11

<211> 20

<212> DNA

<213> 玉米(Zea mays)

<400> 11

agaattagtg ccgcggtgaa 20

<210> 12

<211> 20

<212> DNA

<213> 玉米(Zea mays)

<400> 12

ttgaagcaca gctggtcgat 20

<210> 13

<211> 267

<212> PRT

<213> 玉米(Zea mays)

<400> 13

Met Val Ser Gly Tyr Ala Gln Asn Gly Arg His Glu Glu Ala Val Arg

1 5 10 15

Thr Phe Leu Asp Met Trp Asp Gly Ala Gly Val Arg Pro Asn Glu Leu

20 25 30

Thr Val Ser Ser Val Leu Pro Ala Cys Ala Ala Leu Gly Ala Leu Ala

35 40 45

Leu Gly Arg Lys Val Glu Arg Tyr Ala Arg Gly Arg Gly Met Leu Arg

50 55 60

Asn Val Tyr Val Ala Asn Ala Leu Val Glu Met Tyr Ala Lys Cys Gly

65 70 75 80

Ser Ile His Arg Ala Trp Lys Val Phe Arg Gly Met Gly Thr Gln Arg

85 90 95

Asp Leu Cys Ser Trp Asn Ser Met Ile Met Ala Phe Val Val His Gly

100 105 110

Leu Trp Thr Glu Ala Leu Thr Leu Phe His Lys Leu Arg Met Thr Gly

115 120 125

Ala Lys Pro Asp Gly Ile Thr Phe Val Gly Val Ile Leu Ala Cys Thr

130 135 140

His Gly Gly Leu Val Asp Glu Gly Lys Leu Leu Phe Asn Ser Met Arg

145 150 155 160

Gly Glu Phe Gly Leu Lys Pro Arg Ile Glu His Tyr Gly Cys Met Val

165 170 175

Asp Leu Leu Gly Arg Val Ala Asn Leu Val Ile Leu Ser Asn Ile Tyr

180 185 190

Ala Ser Cys Gly Lys Trp Asp Gly Val Ala Arg Val Trp Lys Leu Leu

195 200 205

Lys Glu Lys Asp His Lys Lys Ser Ala Gly Tyr Asn Val Ile Glu Leu

210 215 220

Asp Gly Arg Met His Lys Phe Leu Val Glu Asp Lys Ser His Pro Arg

225 230 235 240

Ser Glu Lys Val Tyr Asp Ala Leu Asp Ser Ile Thr Leu Ala Met Lys

245 250 255

Leu Val Ser Ser Glu Asn Pro Glu Val Glu Ser

260 265

<210> 14

<211> 804

<212> DNA

<213> 玉米(Zea mays)

<400> 14

atggtgtcgg gctacgcgca gaacgggagg catgaggagg cggtgcgcac gttcctggac 60

atgtgggacg gggccggggt gcggcccaac gagctcaccg tcagcagcgt cctgcccgcg 120

tgcgctgcgc tcggggccct cgcgctgggg aggaaggtgg agcgctatgc cagggggaga 180

gggatgctca ggaacgtcta tgtcgccaac gccttggtcg agatgtacgc caagtgcggc 240

agcatccacc gggcttggaa ggtgtttcgg ggcatgggga cgcagaggga tctttgttct 300

tggaactcca tgatcatggc gtttgtcgtg catggcctct ggacggaggc gctgaccttg 360

tttcataaac tgaggatgac aggggcaaaa ccagatggca ttacatttgt tggagtcatt 420

ttggcctgta ctcatggagg cttggtggat gaaggcaagc tgcttttcaa ctcgatgaga 480

ggggagttcg gtcttaagcc aagaatcgag cactatggtt gcatggttga cctgctgggg 540

cgtgttgcta acctagtgat cctttcgaat atatacgcgt catgtggcaa atgggacggt 600

gttgcccggg tgtggaagct gctaaaggaa aaagatcata agaaatctgc tgggtacaac 660

gttattgagt tagatggtag gatgcacaag ttcctggttg aagataagtc gcatccaaga 720

tctgagaagg tatatgacgc cctggatagc atcacgctgg ccatgaagct tgtcagctcg 780

gaaaatcctg aagtggaaag ctga 804

<210> 15

<211> 18

<212> DNA

<213> 玉米(Zea mays)

<400> 15

ggggcgtgtt ggtctgtt 18

<210> 16

<211> 20

<212> DNA

<213> 玉米(Zea mays)

<400> 16

aagttgccat ggaagtgcag 20

<210> 17

<211> 720

<212> PRT

<213> 玉米(Zea mays)

<400> 17

Met Ala Leu Lys Val Ala Tyr Arg Ile Arg Phe Ala Arg Trp His Leu

1 5 10 15

Leu Ser Gly Arg Leu Met Tyr Cys Ser Cys Pro Asp Ser Pro Ser Ala

20 25 30

Arg Ala Ser Ala Glu Arg Ala Ser Ser Ser Glu Phe Asp Ser Ala Ile

35 40 45

Arg Ser Leu Lys Asp Ser Leu Arg Pro Glu Ser Leu Ala Arg Ile Leu

50 55 60

Asp Ser Thr Ser Asp Ser Ser Leu Ala Leu Arg Ile Phe Arg Trp Ala

65 70 75 80

Ser His Gln Arg Tyr Asn Ile Gln Thr Val Asp Thr Tyr Ser Cys Met

85 90 95

Ile Ser Arg Leu Thr Val Val Glu Asn Arg Asp Glu Met Asp Ser Leu

100 105 110

Leu Ser Glu Met Val Arg Leu Arg Val Pro Ala Leu Glu Gln Ala Leu

115 120 125

Asn Lys Leu Val Glu Ser Leu Ser Ser Lys Asn Arg Cys Asp Glu Ala

130 135 140

Leu Leu Val Ile Gln His Ala Arg Ser Ala Lys Leu Lys Leu Ser Val

145 150 155 160

Ser Ala Cys Asn Gly Val Leu His Gly Met Val Arg Gln Gly Arg Gly

165 170 175

Leu Arg Leu Phe Met Leu Ala Tyr Thr Glu Ile Ile Lys Ala Gly Val

180 185 190

Leu Pro Asp Val Glu Ala Leu Asn Phe Leu Ile Gln Ala Leu Cys Glu

195 200 205

Ser Gly Arg Leu Asp Leu Ala Leu Ile Gln Phe Asp Arg Met Asn Lys

210 215 220

Lys Arg Cys Thr Pro Asn Ser His Thr Phe Glu Ile Leu Ile Thr Ala

225 230 235 240

Leu Cys Phe His Ser Arg Ala Asp Glu Ala Val Lys Leu Phe Asp Lys

245 250 255

Met Leu Gln Leu Arg Cys Ser Pro Asp Ser Ser Phe His Ala Gln Val

260 265 270

Met Pro Leu Phe Cys Lys Phe Ser Lys Val Lys Glu Val Ile Thr Leu

275 280 285

His Arg Ile Met Lys Glu Gly Asn Ile Gln Leu Ser Leu Gln Leu Tyr

290 295 300

Ser Ala Leu Val Arg Cys Leu Cys Glu Asn Glu Leu Leu Asp Asp Ala

305 310 315 320

Thr Ile Val Phe Lys Glu Met Val Ala Ser Gly His Ala Pro Met Ala

325 330 335

Ile Thr Tyr Val Asp Met Ile Asn Cys Tyr Cys Thr Lys Ser Lys Phe

340 345 350

His Asn Ala Met Ile Phe Leu Glu Glu Asn Gly Val Thr Glu Leu Glu

355 360 365

Pro Tyr Asn Val Leu Leu Thr Trp Leu Cys Arg Asn Asp Arg Leu Gln

370 375 380

Asp Ser Val Asn Tyr Leu Glu Lys Leu His Ser Arg Gly Phe Val Asp

385 390 395 400

Cys Glu Ser Trp Asn Ile Val Ile Thr Gln Phe Cys Asn Glu Gly Asn

405 410 415

Ile Arg Arg Ala Phe Glu Leu Ile Gly Arg Met Val Val Ser Ser Phe

420 425 430

Ala Pro Asp Lys Thr Thr Tyr Ser Ala Val Ile Ser Cys Tyr Cys Arg

435 440 445

Leu Gly Ser Tyr Ile Asp Ala Leu Ser Met Phe Arg Arg Val Ser Ile

450 455 460

Ser Asn Met Ser Leu Asn Ser Glu Ser Phe Ser Gln Leu Val Glu Val

465 470 475 480

Leu Cys His Ser Lys Arg Ile Lys Glu Ala Ile Glu Val Phe Lys Tyr

485 490 495

His Tyr Asn Arg Gly Cys Ser Leu Thr Asn Lys Ser Leu Asp Met Leu

500 505 510

Ile Gln Gly Ser Cys Leu Ser Gly Lys Ile Gln Glu Ala Val Lys Leu

515 520 525

Arg Thr Leu Ala Val Ser Val Cys Thr Gly Thr Phe Cys Thr Phe Ser

530 535 540

Thr Tyr Asp Ile Ile Ile Gln Ala Leu Phe His Leu Lys Glu Gly Lys

545 550 555 560

His Val Leu Ala Leu Phe Ala Gln Met Leu Met Glu Gly Cys Pro Leu

565 570 575

Asp Gly Tyr Ala Tyr Asn Ile Leu Leu Arg Ser Phe Leu Thr Lys Glu

580 585 590

Thr Ile Ile Glu Ala Ala Ile Leu Phe Asn Arg Met Val Asn His Ser

595 600 605

Phe Phe Pro Asp Gln Glu Thr Phe Glu Leu Leu Val Asn Asp Met Ala

610 615 620

Leu Phe Ser Phe Leu Asn Met Val Ala Gln Ser Leu Leu Lys Val Val

625 630 635 640

Asp Ala Ser Gly Thr Val Ser Pro Arg Met Tyr Asn Ile Ile Ile Tyr

645 650 655

Gly Leu Ile Lys Glu Gly Phe Lys Ser Glu Ala Cys Lys Phe Leu Asp

660 665 670

Gln Met Leu Glu Lys Gly Trp Val Pro Asp Ser Arg Thr His Arg Val

675 680 685

Leu Val Gly Ser Ser Asp Leu Val Glu Ala Arg Glu Leu Val Glu Ala

690 695 700

Tyr Gln Thr Val Asp Gly Asp Asn Val Ala Asn Ile Leu Leu Glu Gly

705 710 715 720

<210> 18

<211> 2169

<212> DNA

<213> 玉米(Zea mays)

<400> 18

atggcgctga aggttgcgta tcgcatacgc tttgcgagat ggcacctgct atcaggcaga 60

ctcatgtact gttcgtgccc agattcgccc tccgcgcggg cttctgccga gagagcaagt 120

tcttccgagt ttgattctgc gatccggtca ctcaaggaca gtctccggcc agagagtcta 180

gcccggattc tcgattccac gtccgattcg agcttggcac ttaggatctt cagatgggct 240

tcgcatcaga ggtataacat ccagacggtt gatacctaca gctgtatgat ctccaggctt 300

actgttgtgg aaaatcgtga tgagatggac agcctcctca gtgagatggt caggctgagg 360

gttcctgcgc tggagcaggc ccttaataaa ttagttgagt ccctgagcag taagaaccga 420

tgtgatgaag ctttgctggt aatacagcat gctagatctg cgaagcttaa gttgtcagtc 480

tcagcttgca acggagtgct gcatggtatg gtcaggcaag gaaggggttt gcggttgttc 540

atgcttgcgt acacggagat tatcaaggcg ggagtcctcc ctgatgtgga ggcattgaat 600

tttctgatcc aggctttgtg tgagtctggt cgtttagatc tggcactgat tcagtttgac 660

aggatgaaca agaagaggtg tactcctaat agtcatacgt ttgagatact gatcacagca 720

ctttgtttcc acagcagagc agatgaagcg gtcaagctct ttgataagat gctgcaactt 780

agatgctccc ctgatagcag cttccatgct caagtgatgc ccctgttttg caagttcagt 840

aaagttaagg aggtgattac gttgcatcga ataatgaaag aaggcaacat tcagttgagt 900

ctgcagttgt acagtgcctt ggttagatgt ttatgtgaga atgaactact ggatgacgct 960

accatagtgt tcaaagagat ggtagcatca ggtcatgctc caatggcaat tacatatgtg 1020

gacatgataa attgctactg tacaaaatcc aagttccaca acgctatgat ctttctagag 1080

gaaaatggtg tcactgaatt agaaccatat aatgtgctcc tgacatggtt gtgtagaaat 1140

gacaggctgc aggattcagt aaactatctt gagaaattgc acagcagagg ttttgttgat 1200

tgtgagtcat ggaacattgt tataactcag ttctgcaatg aaggaaacat cagaagagca 1260

tttgagctca ttggtagaat ggttgtttct tcatttgcac cagataagac tacctattct 1320

gctgttatat cttgttactg tcgattagga tcttacatag atgccttgag tatgttcaga 1380

agggtaagta ttagcaacat gtctttgaat tcggaatctt tctcacagct tgtagaggtt 1440

ttgtgtcact caaagaggat caaagaagct atcgaagttt ttaaatatca ctataataga 1500

ggttgcagtc tgacaaacaa gtcacttgat atgctaattc aagggagttg cttgtctgga 1560

aagattcaag aagcagtcaa attgcgcacg ttggctgttt ctgtttgcac aggcactttt 1620

tgtacattct ccacttatga cataattatc caagcactgt tccatttgaa ggaggggaaa 1680

catgtattgg ctctctttgc acaaatgttg atggaaggtt gtccattgga tgggtatgca 1740

tacaatattc tgttgcgctc tttcctcacc aaggaaacaa ttattgaggc tgctatcttg 1800

ttcaacagaa tggttaacca ttcctttttt cctgaccaag agacgtttga attattagtc 1860

aatgatatgg ccttgttttc ttttcttaat atggttgcac aaagtttact gaaagtggta 1920

gatgcaagtg gaacagtgag ccctagaatg tataacatta ttatttacgg acttattaaa 1980

gaaggcttca agagtgaggc atgcaagttt ctagaccaaa tgctggaaaa ggggtgggtg 2040

cctgattcaa ggacacatcg tgtcttggtc ggcagctctg atctggtgga ggctagagaa 2100

cttgttgaag cctaccagac agtggatggt gataatgtgg ccaacatatt gttggaagga 2160

ctagattga 2169

<210> 19

<211> 20

<212> DNA

<213> 玉米(Zea mays)

<400> 19

catacgcttt gcgagatggc 20

<210> 20

<211> 20

<212> DNA

<213> 玉米(Zea mays)

<400> 20

agtgaccgga tcgcagaatc 20

<210> 21

<211> 1098

<212> PRT

<213> 玉米(Zea mays)

<400> 21

Met Ala Arg Ser Asn Ser Trp Tyr Val Leu His Phe Cys Ser Thr Ser

1 5 10 15

Leu Lys Ser Trp Pro Ser Ser Ala Thr Asp His Pro His Leu Phe Pro

20 25 30

Gln Ile Arg Cys Arg Leu Leu Ser Leu Pro Phe Leu Arg Arg Gly Ile

35 40 45

His Ser Ser Val Pro Thr Ser Val Gly Ala Ala Thr Asp Ala Thr Leu

50 55 60

Leu Gly Arg Leu Thr Arg Leu Leu Leu Leu His Arg Phe Ser Ala Val

65 70 75 80

Ala Arg Leu Leu Ser Ser Ser Ala Pro Leu Thr Pro Ala Leu Leu His

85 90 95

Ala Ala Leu Arg Arg Val Arg Leu Asp Ala Asp Ala Ala Val His Leu

100 105 110

Phe Arg Leu Ala Pro Phe Pro Pro Ser Leu Leu Ala His Ala Gln Leu

115 120 125

Leu His Ile Leu Val Arg Ala Arg Arg Ser Ala Asp Ala Arg Ala Ile

130 135 140

Leu Ala Ser Leu Leu Ser Ala Arg Pro Pro Ala Pro Pro Leu Phe Pro

145 150 155 160

His Leu Val Glu Val Tyr Lys Glu Phe Ser Phe Ser Ala Ala Ser Phe

165 170 175

Asp Leu Leu Leu Arg Ala Leu Ala Asn Ala Gly Gln Leu Asp Gly Ala

180 185 190

Leu Gln Val Phe Asp Glu Met Arg Thr Leu Gly Cys Arg Leu Ser Met

195 200 205

Arg Ser Cys Asn Ser Ile Leu Asn Arg Leu Ala Gln Thr Gly Asp Leu

210 215 220

Gly Ala Thr Val Ala Val Phe Glu Gln Met Gln Arg Ala Gly Ala Leu

225 230 235 240

Pro Asp Lys Phe Thr Val Ala Ile Met Ala Lys Ala Tyr Cys Lys Val

245 250 255

Lys Gly Val Val His Ala Leu Glu Phe Val Glu Glu Met Thr Lys Met

260 265 270

Gly Val Glu Val Asn Leu Val Ala Tyr His Ala Val Met Asn Gly Tyr

275 280 285

Cys Glu Val Gly Gln Thr Asn Asp Ala Arg Arg Met Leu Asp Ser Leu

290 295 300

Pro Gly Arg Gly Phe Ser Pro Asn Ile Val Thr Tyr Thr Leu Leu Val

305 310 315 320

Lys Gly Tyr Cys Asn Glu Lys Asn Met Glu Glu Ala Glu Gly Val Val

325 330 335

Gln Glu Ile Arg Lys Asn Lys Gln Leu Val Val Asp Glu Ala Val Phe

340 345 350

Gly Ala Val Ile Asn Gly Tyr Cys Gln Met Gly Arg Met Glu Asp Ala

355 360 365

Ala Arg Leu Leu Asn Glu Met Val Asp Ser Arg Leu Gln Val Asn Leu

370 375 380

Phe Val Tyr Asn Ile Met Ile Asn Gly Tyr Cys Lys Leu Gly Arg Met

385 390 395 400

Val Glu Ala His Asn Ile Leu His Glu Met Thr Gly Val Gly Val Arg

405 410 415

Pro Asp Thr Tyr Ser Tyr Asn Ser Leu Val Asp Gly Tyr Cys Lys Lys

420 425 430

Gly Leu Met Asn Lys Ala Phe Glu Thr Tyr Asn Thr Met Leu Arg Asn

435 440 445

Gly Phe Ala Ala Thr Thr Leu Thr Tyr Asn Ala Leu Leu Lys Gly Phe

450 455 460

Cys Ser Leu Gly Ser Ile Asp Asp Ala Leu Arg Leu Trp Phe Leu Met

465 470 475 480

Leu Lys Lys Gly Ile Ala Pro Asn Glu Ile Ser Cys Ser Thr Leu Leu

485 490 495

Asp Gly Phe Phe Lys Ser Gly Lys Thr Glu Lys Ala Leu Asn Leu Trp

500 505 510

Lys Glu Thr Leu Ala Arg Gly Leu Ala Lys Asn Thr Thr Thr Phe Asn

515 520 525

Thr Val Ile Asn Gly Leu Cys Lys Ile Glu Arg Met Pro Glu Ala Glu

530 535 540

Glu Leu Val Asp Lys Met Lys Gln Trp Arg Cys Pro Pro Asp Ile Ile

545 550 555 560

Thr Tyr Arg Thr Leu Phe Ser Gly Tyr Cys Lys Ile Gly Asp Met Asp

565 570 575

Arg Ala Ser Arg Ile Leu Asn Glu Leu Glu Asn Leu Gly Phe Ala Pro

580 585 590

Thr Ile Glu Phe Phe Asn Ser Leu Ile Thr Gly His Phe Ile Ala Lys

595 600 605

Gln His Gly Lys Val Asn Asp Ile Leu Phe Glu Met Ser Asn Arg Gly

610 615 620

Leu Ser Pro Asn Thr Val Ala Tyr Gly Ala Leu Ile Ala Gly Trp Cys

625 630 635 640

Lys Glu Gly Asp Leu His Thr Ala Tyr Asn Leu Tyr Leu Glu Met Ile

645 650 655

Glu Lys Gly Leu Val Pro Asn Leu Phe Ile Cys Ser Ser Leu Val Ser

660 665 670

Cys Phe Tyr Arg Lys Gly Lys Phe Asp Glu Ala Asn Leu Val Leu Gln

675 680 685

Asn Leu Val Gly Thr Asp Met Ile Pro Asp Ile Ser Ala Pro Arg Leu

690 695 700

Glu Ile Gly Lys Val Ala Asn Phe Ile Asp Thr Val Ala Gly Gly Asn

705 710 715 720

His His Ser Ala Lys Ile Met Trp Asn Ile Val Ile Phe Gly Leu Cys

725 730 735

Lys Leu Gly Arg Ile Glu Asp Ala Lys Asn Leu Leu Ala Asp Leu Lys

740 745 750

Asp Lys Gly Phe Val Ala Asp Asn Phe Thr Tyr Ser Ser Leu Ile His

755 760 765

Gly Cys Ser Ala Ser Gly Phe Val Asp Val Ala Phe Asp Leu Arg Asp

770 775 780

Thr Met Leu Ser Val Gly Leu Thr Pro Asn Ile Val Thr Tyr Asn Ser

785 790 795 800

Leu Ile Tyr Gly Leu Cys Lys Ser Gly Glu Leu Ser Arg Ala Val Ser

805 810 815

Leu Phe Lys Lys Leu Trp Thr Lys Gly Ile Ser Pro Asn Ala Ile Thr

820 825 830

Tyr Asn Thr Leu Ile Asp Lys His Cys Lys Asp Gly Tyr Ile Thr Glu

835 840 845

Ala Phe Lys Leu Lys Gln Arg Met Ile Glu Glu Gly Ile His Pro Thr

850 855 860

Val Phe Thr Tyr Ser Ile Leu Ile Asn Gly Leu Cys Thr Gln Gly Tyr

865 870 875 880

Met Glu Glu Ala Ile Lys Leu Leu Asp Gln Met Ile Glu Asn Asn Val

885 890 895

Asp Pro Asn Tyr Val Thr Tyr Trp Thr Leu Ile Gln Gly Tyr Val Arg

900 905 910

Cys Glu Ser Val Asp Val Pro Ile Met Pro Leu Asp Gln Ser Thr Ile

915 920 925

Val Val Lys Ala Ser Pro Arg Arg Ala Val Arg Arg Pro Gly Ser Val

930 935 940

Ser Arg Pro Ala Arg Leu Thr Pro Ala Pro Lys Ala Ser Gly Arg Asp

945 950 955 960

Tyr Val Leu Asn Met Gln Val Ser Ile Thr Phe Gln Gln Tyr Asn Cys

965 970 975

Leu Ile Val Phe Cys Glu Gly Ala Trp Ser Tyr His Glu Lys Cys Phe

980 985 990

Gln Pro Pro Ser His Val Ser Gln Val Val Ser Ser Asn Tyr Arg Leu

995 1000 1005

Asp Trp Leu Ser Lys Cys Arg Asp Leu Leu Leu Val Thr Leu Pro Leu

1010 1015 1020

Leu Ser Gly Thr Arg Tyr Ile Pro Ser Ile Leu Gln Ser Val Ser Tyr

1025 1030 1035 1040

Pro Arg Leu Phe Phe Met Asp Gln Ala Leu Ser Lys Val Tyr Pro Leu

1045 1050 1055

Phe Ile Pro Arg Lys Asn Val Cys Thr Gly Gly Glu Glu Thr Pro Leu

1060 1065 1070

Thr Glu Ser Gly Ser Ser Tyr Phe Gly Ala Asp Ser Gly Phe Cys Gln

1075 1080 1085

Glu Thr Val Phe Trp Phe Gln Glu Lys Gln

1090 1095

<210> 22

<211> 3297

<212> DNA

<213> 玉米(Zea mays)

<400> 22

atggcgcgaa gcaacagctg gtacgttctg cacttctgct ccacctccct aaaatcttgg 60

ccgagctccg ccactgacca tccccacttg tttccccaga tacgctgccg cctcctatcg 120

ctccccttcc tccgccgcgg catccactcc tccgtgccaa cttcggtcgg cgccgctaca 180

gatgctacac tcctgggtcg cctcacccgc ctcctcctcc tccaccgttt ctctgccgtc 240

gctcgcctcc tctcctcctc ggcccccctc acccctgcac tcctccacgc ggcgctgcgc 300

cgggtccgcc tcgacgccga cgccgcggtg cacctcttcc gcctcgcccc tttcccccct 360

tccctcctcg cgcacgccca gctgctccac atcctcgtcc gcgcccggcg ctccgccgac 420

gcccgcgcga tcctggcatc cctcctctcc gcacggcccc cggctccgcc tctctttccg 480

cacctcgtcg aggtatataa ggagttctcc ttctccgcag cctcattcga cctgctcctc 540

cgtgcacttg ccaacgccgg ccaactcgac ggcgccctcc aggtgttcga cgaaatgcga 600

acgctcgggt gccgccttag catgcggtcc tgcaacagta tactcaacag gttagcacag 660

actggggacc tgggcgccac agtggcagtg tttgagcaga tgcagcgtgc aggggctctg 720

ccagacaagt tcacggtggc gattatggca aaggcttact gcaaggttaa gggggtggtg 780

catgcattgg agttcgtgga ggagatgaca aagatgggtg tggaggtgaa cctagtggcg 840

taccatgcgg tgatgaatgg ctattgtgag gtggggcaga ccaacgatgc aagaaggatg 900

ttggactcgc tgcctgggag aggtttttca ccgaatatag ttacatatac attgcttgtg 960

aagggatatt gtaatgagaa gaacatggag gaggctgagg gggttgttca ggagattagg 1020

aagaataagc agcttgtggt tgatgaggct gtttttggtg cagtgatcaa tggctattgc 1080

caaatgggaa gaatggagga tgcagctcga ttattgaatg agatggtgga ttctaggctc 1140

caggttaatt tgtttgtgta taacataatg attaatggat attgcaagtt ggggagaatg 1200

gtggaagcac ataacatttt gcatgaaatg acaggtgttg gtgtgaggcc tgacacatac 1260

agttacaata gcctagttga tgggtattgc aaaaagggtt tgatgaacaa ggctttcgaa 1320

acatataata caatgttaag gaatggattc gcggcgacga cattaacata taatgccctc 1380

ctaaaaggtt tttgctcgct tggctcaatc gatgatgcgc tcagactttg gttcctgatg 1440

ttgaagaaag gtattgcacc taatgaaatc agttgtagca ccttgcttga tggtttcttc 1500

aagtcaggta aaactgagaa ggccttaaat ctttggaaag aaaccttagc aaggggttta 1560

gcaaaaaata caaccacatt taacacagtt atcaatgggt tgtgtaagat tgagaggatg 1620

cctgaagcag aagagcttgt tgacaaaatg aagcaatgga gatgtcctcc tgatattata 1680

acttacagaa cattatttag tggttattgt aagataggtg acatggatag agctagccgc 1740

attctgaatg aactggaaaa tttaggtttt gctcctacaa ttgaattttt caattctttg 1800

ataactggac acttcatagc taagcaacat ggaaaggtga atgatatact ctttgaaatg 1860

agtaataggg gactttctcc taacacggtc gcatatggag ctctgattgc tggatggtgt 1920

aaagagggag atttgcatac agcctataac ttatatcttg aaatgattga gaaaggtctg 1980

gtaccaaatc tttttatttg cagttcttta gtgagttgtt tctacaggaa gggaaagttt 2040

gatgaggcaa atttagtgtt gcaaaatctt gtgggtactg acatgattcc agatatcagt 2100

gcgcccagac ttgagatagg gaaagtagca aatttcattg ataccgttgc tggtggaaat 2160

catcattctg caaaaatcat gtggaacatt gttatttttg gtctatgcaa attgggaagg 2220

attgaagatg ctaaaaactt gttggcagat ctaaaagaca aggggtttgt tgctgataat 2280

ttcacttatt ctagcctcat ccatggttgt tctgcttcag gatttgttga tgtagctttt 2340

gatttgaggg atacaatgtt gagtgttggt ctcactccaa atatagtaac ctacaattct 2400

cttatatatg gtctctgcaa gtctggggag ttatccaggg ctgtcagtct ttttaagaag 2460

ttgtggacaa agggtatatc ccctaatgct atcacatata atacattaat tgataagcat 2520

tgcaaggatg gttacataac cgaggccttt aaactaaagc aaaggatgat agaggaaggt 2580

attcatccta ctgtgttcac ctattctata ctgattaatg gtctctgtac tcaaggctat 2640

atggaagaag caatcaagct tcttgatcaa atgattgaga ataatgtgga tccaaattat 2700

gtcacatatt ggacactgat tcaaggttat gttagatgtg aaagtgttga tgttccaata 2760

atgccactag atcaatcaac catagttgtt aaggcgtcgc ctaggcgggc ggtaaggcgc 2820

cccggcagcg taagtcgtcc tgctcgcctt actccagcac ctaaggcgag tgggagagac 2880

tatgtgctca acatgcaagt ctcaatcact ttccagcaat ataattgtct aattgtcttc 2940

tgcgaagggg cctggtcata tcatgagaaa tgtttccaac ctccctcaca tgtcagccaa 3000

gtggtatctt ctaactacag gctggattgg ctttctaagt gcagagactt gttgctggtg 3060

actttgcccc ttttatctgg tacacgctac attccatcaa tattgcagtc cgtatcctat 3120

ccacgattgt tcttcatgga tcaggcttta agcaaggttt accctctttt tatacctagg 3180

aaaaatgttt gcacaggtgg tgaagaaacc ccattgacag aaagtggttc gagttacttt 3240

ggagctgact ctggattctg tcaagaaaca gtcttttggt tccaagagaa gcaatag 3297

<210> 23

<211> 20

<212> DNA

<213> 玉米(Zea mays)

<400> 23

catggataga gctagccgca 20

<210> 24

<211> 20

<212> DNA

<213> 玉米(Zea mays)

<400> 24

tgcgaccgtg ttaggagaaa 20

<210> 25

<211> 501

<212> PRT

<213> 玉米(Zea mays)

<400> 25

Met Leu Met Pro Pro Pro Pro Cys Pro Pro Thr Leu Pro His Leu Leu

1 5 10 15

Arg His Leu Arg Gly Arg Met Leu Thr Thr Gln Leu Leu Asp His Leu

20 25 30

Ile Arg Ser Thr Ser Ser Ser Pro Phe Pro Ser Leu Ser Phe Ser Val

35 40 45

Phe Ile Leu Leu Leu Arg Ser Ser Leu Arg Pro Ser His Phe Thr Phe

50 55 60

Pro Phe Leu Ala Arg Ala Ala Ala Arg Leu Ser Ser Ala Ser Leu Ala

65 70 75 80

Ser Ala Leu His Ala His Pro Leu Arg Leu Gly Leu Leu Pro Ala Asp

85 90 95

Leu His Val Ala Asn Ser Leu Val His Met Tyr Ala Ala Cys Ala Leu

100 105 110

Pro Asp His Ala Asn Arg Val Phe Asp Glu Ile Pro Arg Pro Asn Leu

115 120 125

Val Ser Trp Asn Ala Leu Leu Asp Gly Tyr Ala Lys Cys His Asp Leu

130 135 140

Pro Ala Ala Arg Lys Val Phe Ala Arg Met Pro Gln Arg Asp Val Val

145 150 155 160

Ser Trp Ser Ala Met Ile Asp Gly Cys Val Lys Cys Gly Glu His Arg

165 170 175

Glu Ala Leu Ala Leu Phe Glu Met Met Glu Asn Ala Ala Ala Arg Ser

180 185 190

Ala Glu Glu Glu Asp Gly Gly Gly Gly Ala Arg Ala Asn Asp Val Thr

195 200 205

Met Val Ser Val Leu Gly Ala Cys Ala Gln Leu Gly Asp Leu Glu Arg

210 215 220

Gly Arg Arg Ala His Arg Cys Leu Arg Glu Arg Gly Phe Ala Leu Asn

225 230 235 240

Leu Arg Leu Ala Thr Ser Leu Val Asp Met Tyr Ala Lys Cys Gly Ala

245 250 255

Ile Ser Glu Ala Met Glu Val Phe Trp Ala Val Pro Val Glu Ser Thr

260 265 270

Asp Val Leu Ile Trp Asn Ala Val Ile Gly Gly Leu Ala Val His Gly

275 280 285

Met Ala Thr Lys Ser Val Glu Ile Phe Leu Glu Met Gln Arg Ala Gly

290 295 300

Val Ala Pro Asp Glu Ile Thr Tyr Leu Cys Leu Leu Ser Ala Cys Ala

305 310 315 320

His Gly Gly Leu Val Asp Glu Ala Trp Glu Phe Phe Arg Ser Leu Glu

325 330 335

Ala Gln Gly Leu Arg Pro His Val Glu His Tyr Ala Cys Leu Val Asp

340 345 350

Val Leu Gly Arg Ala Gly Arg Leu Glu Glu Val Tyr Gly Val Val Lys

355 360 365

Ser Met Pro Met Lys Pro Ser Val Ser Val Leu Gly Ala Leu Leu Asn

370 375 380

Ala Cys His Leu His Gly Trp Val Glu Leu Gly Glu Val Leu Gly Arg

385 390 395 400

Arg Leu Val Gln Leu Gln Pro Asp His Asp Gly Arg Tyr Ile Gly Leu

405 410 415

Ser Asn Ile Tyr Ala Val Ala Arg Arg Trp Gln Glu Ala Lys Lys Ala

420 425 430

Arg Lys Val Met Glu Asp Arg Gly Val Lys Lys Val Pro Gly Phe Ser

435 440 445

Glu Ile Asp Val Gly Gly Gly Leu Ser Arg Phe Thr Ala Gln Asp Lys

450 455 460

Thr His Pro Gly Ser Val Lys Ile Tyr Glu Leu Leu Asn Leu Ile Ala

465 470 475 480

Ile Glu Met Lys Met Lys Asp Asp Ala Thr Ile Pro Asp Tyr Leu Leu

485 490 495

Cys Thr Leu Leu Glu

500

<210> 26

<211> 1506

<212> DNA

<213> 玉米(Zea mays)

<400> 26

atgctcatgc ctccgcctcc ctgcccgccg acgctgccgc accttctccg ccacctccgc 60

ggccgcatgc tcaccaccca gctcctcgac cacctcatca ggtcaacctc atcctcgccg 120

ttcccctccc tctccttctc cgtcttcatc ctcctcctcc gctcctccct ccgcccgtcc 180

cacttcactt tcccattcct tgcccgcgcc gccgcgcgcc tttcctccgc ctccctcgcg 240

tctgcgctcc acgcgcaccc gctgcggctc gggctcctcc ccgccgacct ccacgtcgcc 300

aactccctcg tccacatgta cgcggcctgc gccctgccgg accacgccaa ccgagtgttc 360

gacgaaatcc cgcgccccaa ccttgtctcc tggaacgcgc tcctcgatgg gtacgccaag 420

tgccacgacc tccccgctgc gcgaaaggtg ttcgctcgga tgccccagcg ggacgtggtg 480

tcctggagcg ccatgatcga cgggtgcgtc aagtgtgggg agcaccgcga ggcgctcgcg 540

ctgttcgaga tgatggagaa tgctgccgcc aggtcagcag aggaggagga tggtgggggt 600

ggggcgaggg ctaacgacgt cacgatggtg agcgtgcttg gagcctgtgc tcagcttgga 660

gacctcgagc gcggaaggcg ggcgcaccgg tgcctgaggg aacgtggctt tgcattgaac 720

ctcaggcttg caacctcgct cgttgacatg tatgccaagt gtggggccat cagtgaggca 780

atggaggtgt tctgggctgt gccggtggag agcaccgatg ttctgatatg gaatgctgtg 840

atcggtggcc ttgcggtgca tggcatggcc accaaatcgg tggagatatt cctggagatg 900

cagcgtgctg gggttgcgcc ggatgagatc acgtaccttt gtctgctgag cgcttgtgcc 960

cacggcggtc tagtggatga ggcttgggag ttcttccgct cacttgaagc gcaagggctc 1020

agaccacatg tcgagcatta tgcttgcctg gtagatgtgc ttggcagagc aggacgcctg 1080

gaggaagtgt acggtgttgt taaaagtatg ccaatgaaac ctagtgtttc tgtgcttggt 1140

gctctcttaa atgcctgtca tttgcatgga tgggtggagc tgggcgaggt actcggtagg 1200

cggcttgtcc agttgcagcc agaccacgat ggcaggtaca ttggcctgtc caatatctat 1260

gctgttgcca gacggtggca agaggcaaag aaagctagaa aggtgatgga ggataggggt 1320

gtaaaaaagg tccctggatt tagtgagatt gatgttggtg gagggctctc caggttcact 1380

gcccaggaca agactcatcc tggctcagtg aagatctatg agttgctgaa tctaatagca 1440

atagagatga aaatgaagga tgatgctact attccagatt acctcctgtg cacactgcta 1500

gaatga 1506

<210> 27

<211> 20

<212> DNA

<213> 玉米(Zea mays)

<400> 27

ttgggagttc ttccgctcac 20

<210> 28

<211> 20

<212> DNA

<213> 玉米(Zea mays)

<400> 28

ccaagcacat ctaccaggca 20

Claims (6)

1.玉米PPR蛋白或其编码基因在提高植物抗寒性中的应用；

所述玉米PPR蛋白的氨基酸序列如SEQ ID NO.1所示；所述植物为玉米。

2.玉米PPR蛋白或其编码基因在抗寒植物育种或抗寒转基因植物制备中的应用；

所述玉米PPR蛋白的氨基酸序列如SEQ ID NO.1所示；所述植物为玉米。

3.根据权利要求1或2所述的应用，其特征在于，所述玉米PPR蛋白由如SEQ ID NO.2所示的核苷酸序列编码得到。

4.一种提高植物抗寒性的方法，其特征在于，包括：

提高植物中玉米PPR蛋白的表达水平，所述玉米PPR蛋白的氨基酸序列如SEQ ID NO.1所示；所述植物为玉米。

5.根据权利要求4所述的方法，其特征在于，通过提高所述植物中所述玉米PPR蛋白的编码基因的表达水平，提高所述植物的抗寒能力；或，通过将所述玉米PPR蛋白的编码基因过表达的株系与其他株系杂交，培育抗寒株系。

6.根据权利要求5所述的方法，其特征在于，提高所述植物中所述玉米PPR蛋白的编码基因的表达水平，具体为：

将包含所述玉米PPR蛋白的编码基因的载体转化至农杆菌EHA105菌株中，再浸染玉米愈伤组织，得到转基因植株的幼苗。