CN114432164A

CN114432164A - Aqueous face mask

Info

Publication number: CN114432164A
Application number: CN202011220859.5A
Authority: CN
Inventors: 曲召辉; 刘盼玉; 刘有停
Original assignee: Taihe Kangmei Beijing Research Institute of Traditional Chinese Medicine Co Ltd
Current assignee: Taihe Kangmei Beijing Research Institute of Traditional Chinese Medicine Co Ltd
Priority date: 2020-11-05
Filing date: 2020-11-05
Publication date: 2022-05-06
Anticipated expiration: 2040-11-05
Also published as: CN114432164B

Abstract

The invention discloses an aqueous face mask, which comprises the following raw materials in parts by weight: 82.4% -99.3% of a white fungus and dendrobium extract, 0.00% -10.00% of a humectant, 0.05% -2.5% of a preservative, 0.02% -0.10% of a chelating agent and 0.00% -5.00% of a skin feel regulator, wherein the preparation process of the white fungus and dendrobium extract comprises the following steps: mixing the following components in parts by weight: 1-5: the tremella and dendrobium are soaked in water according to the weight ratio of 1, then elutriated, and extracted under the conditions that the extraction pressure is 40-130 KPa, the extraction temperature is 105-125 ℃, and the extraction time is 1-4 hours to obtain the tremella and dendrobium extract. The tremella and dendrobium extract is added into the mask liquid of the mask, so that the skin feel is improved, the skin-friendly property, the fitting property and the like of mask cloth are improved, the whole mask achieves the effect of excellent skin feel, and the water aqua mask also has good moisturizing effect and can remarkably improve the elasticity and the glossiness of skin.

Description

Aqueous face mask

Technical Field

The invention belongs to the field of cosmetics, and particularly relates to an aqueous face mask.

Background

The facial mask is a product which is coated or applied on the surface of human skin, and is removed, scrubbed or kept after a period of time to play a role in centralized nursing or cleaning. The facial mask is mainly classified into a face mask, a cream mask, a gel mask and a powder mask according to the product type, wherein the face mask occupies more than 80% of the mask market. The mask can quickly replenish water and moisturize, and has good effects on repairing after being dried and replenishing water and calming. It is popular with people because of its convenient use and obvious effect.

The mask is a patch type product to be applied on the face for 10-15 minutes, and thus the use comfort and skin feel thereof are properties of great concern to consumers. The face mask is formed by combining face mask cloth and facial mask liquid, and the use feeling of the face mask is directly related to both the face mask cloth and the facial mask liquid. The materials of the film-pasting base cloth (hereinafter referred to as 'mask cloth') on the market mainly comprise hydrogel, silk, tencel, biological fiber, bamboo charcoal fiber, pure cotton fiber, viscose fiber, cuprammonium fiber, binchotan fiber, chitosan fiber and the like. At present, most mask products on the market can achieve certain beautifying and skin-care effects, but the comfort and skin-friendly feeling of the mask products cannot meet the requirements of consumers.

Chinese patent CN106236631B discloses a facial mask solution for lightening skin color comprising: 1.65-7.2% of a brightening composition, 0.05-0.2% of a chelating agent, 0.02-0.1% of a neutralizing agent, 0.1-0.5% of an acrylic acid (ester)/C10-30 alkanol acrylate cross-linked polymer, 0.05-0.2% of xanthan gum, 5.1-17.8% of a humectant and the balance of deionized water, wherein the brightening composition is selected from a combination of more than two of nicotinamide, acetyl chitosamine, carnosine and a dendrobium extract, and contains nicotinamide and acetyl chitosamine. The facial mask liquid not only has excellent deep moisturizing effect, but also has obvious effects of color homogenizing and brightening dull and yellowish skin color, so that the skin is closer to a natural and bright state; chinese patent CN106727148A discloses a dendrobium officinale moisturizing and repairing mask comprising: the A phase component comprises a humectant, a preservative, a thickening agent, sodium hyaluronate and water, and the B phase component comprises a dendrobium officinale extract, a American ginseng root extract, a cosolvent and water. Although the above documents disclose that the dendrobium facial mask liquid has whitening and moisturizing effects, whether the facial mask liquid can improve the comfort and skin-friendly feeling of the facial mask is not studied.

The development of the plant ingredient skin-friendly aqueous mask can well meet the requirements of consumers on the comfort and skin-friendly feeling of the mask products on the market.

Disclosure of Invention

Based on the defects of the prior art, the invention aims to provide the water aqua mask with plant ingredients for improving the skin-friendly property of the mask cloth.

The technical problem to be solved by the invention is realized by the following technical scheme:

according to the invention, the aqueous face mask comprises the following raw materials in parts by weight: 82.4-99.3% of tremella and dendrobium extract, 0.00-10.00% of humectant, 0.05-2.5% of preservative, 0.02-0.10% of chelating agent and 0.00-5.00% of skin feel regulator.

According to the invention, the tremella-dendrobium extract is prepared from tremella and dendrobium according to a certain proportion.

According to the invention, the preparation method of the tremella-dendrobium extract comprises the following steps: the tremella and dendrobium extract is prepared by adopting a high-temperature and high-pressure method.

According to a further embodiment of the invention, the preparation method of the tremella-dendrobium extract specifically comprises the following steps:

soaking white fungus and dendrobium in water according to a certain weight part ratio, washing, and extracting to obtain a white fungus and dendrobium extract;

in the extraction step, the extraction pressure is 40-130 KPa; the extraction temperature is 105-125 ℃; the extraction time is 1-4 h.

According to a further embodiment of the invention, the weight ratio of tremella to dendrobium in the tremella-dendrobium extract is 1: 1-5: 1.

According to a further embodiment of the invention, in the extracting step of the tremella and dendrobium extract, the extracting pressure is 40-75 KPa.

According to a further embodiment of the invention, in the step of extracting the tremella and dendrobium extract, the extraction temperature is 105-115 ℃.

According to a further embodiment of the invention, in the step of extracting the tremella and dendrobium extract, the extraction time is 2-3 hours.

According to still further preferred embodiments of the present invention, in the extraction method, the extraction pressure is 70 KPa.

According to still further preferred embodiments of the present invention, in the extraction method, the extraction temperature is 115 ℃.

According to still further preferred embodiments of the present invention, in the extraction method, the extraction temperature and time are 3 hours.

According to a further embodiment of the invention, in the extracting step of the dendrobium candidum extract, the ratio of the dendrobium candidum to water is 1: 30-1: 70.

According to some embodiments of the present invention, the humectant is one or more selected from glycerin, 1, 3-propanediol, and polyhydric alcohols.

According to some embodiments of the invention, the preservative is one or more of those conventional in the art.

According to some embodiments of the present invention, the chelating agent is one or more of sodium glucoheptonate, disodium EDTA, trisodium EDTA and tetrasodium EDTA.

According to some embodiments of the present invention, the skin-feel modifier is one or more of glyceryl polyether-26, panthenol, and other aqueous skin-feel modifiers.

According to the invention, the aqueous mask can be prepared by adopting a conventional method of masks in the field.

According to some embodiments of the invention, the aqueous mask is prepared by the following steps:

taking 82.4-99.3% of tremella and dendrobium extract, 0.00-10.00% of humectant (one or more of glycerin, 1, 3-propylene glycol and polyhydric alcohol), 0.05-2.5% of preservative, 0.02-0.10% of chelating agent (one or more of sodium glucoheptonate, disodium EDTA, trisodium EDTA and tetrasodium EDTA) and 0.00-5.00% of skin feel regulator (one or more of glycerin polyether-26, panthenol and skin feel regulators of other water aqua) as phase A, sequentially adding the phase A raw materials, and stirring uniformly to obtain the water aqua mask.

The invention has the beneficial effects that:

according to the water aqua facial mask, the tremella and dendrobium extract prepared by the method is added, so that the skin feel is improved, and the skin affinity, the fitting property and the like of the mask cloth are improved, so that the whole facial mask achieves an excellent skin feel effect.

The water aqua mask also has good moisturizing effect, and can obviously improve the elasticity and glossiness of the skin.

Drawings

FIG. 1 is a comparison graph of skin feel of Tremella and the Tremella and herba Dendrobii extract of two preparation processes;

FIG. 2 is a skin-feel radar chart of Tremella herba Dendrobii extract with different addition ratios;

FIG. 3 is a graph showing the result of ABTS free radical scavenging experiments with different amounts of Tremella herba Dendrobii extract;

FIG. 4 is a diagram showing the results of skin moisture content tests of 90% Tremella herba Dendrobii extract and facial mask liquid containing 90% Tremella herba Dendrobii extract;

FIG. 5 is a skin elasticity test chart of 90% Tremella herba Dendrobii extract and facial mask liquid containing 90% Tremella herba Dendrobii extract;

FIG. 6 is a skin glossiness test chart of 90% Tremella herba Dendrobii extract and facial mask liquid containing 90% Tremella herba Dendrobii extract;

FIG. 7 is a graph showing the initial feeling evaluation of aqueous mask-pasting mask liquid;

FIG. 8 is a skin feel evaluation chart of the water-based patch;

FIG. 9 is a sample result chart of skin feel after a mask cloth (cotton cloth) is soaked with water, a commercially available mask solution, a water-extracted Tremella herba Dendrobii mask solution and the Tremella herba Dendrobii mask solution of the present invention, respectively;

FIG. 10 is a sample result chart of skin feel after a mask cloth (flannelette) is soaked with water, a commercially available mask solution, a water-extracted tremella-dendrobium mask solution and the tremella-dendrobium mask solution of the present invention, respectively;

FIG. 11 is a result chart of skin feel tests after a mask cloth (copper ammonia wire) is soaked by water, a commercially available mask solution, a water-extracted tremella-dendrobium mask solution and the tremella-dendrobium mask solution of the present invention respectively;

FIG. 12 is a result chart comparing the consistencies of the tremella and dendrobium facial mask liquid, the commercially available facial mask liquid, the water-extracted tremella and dendrobium facial mask liquid and the water-extracted facial mask liquid according to the present invention;

FIG. 13 is a graph of the molecular weight distribution of dendrobe polysaccharides;

FIG. 14 is a graph of molecular weight distribution of Tremella polysaccharides;

FIG. 15 is a molecular weight distribution diagram of Dendrobium officinale polysaccharide prepared from Tremella fuciformis of the present invention.

Detailed Description

The invention is further illustrated below with reference to specific examples, to which, however, the invention is not restricted.

It should be appreciated by those skilled in the art that the present invention is not limited to the above embodiments, and any changes and modifications to the present invention are within the scope of the present invention.

The experimental methods described in the following examples are all conventional methods unless otherwise specified; the experimental materials and reagents are commercially available, unless otherwise specified.

TABLE 1 raw materials and sources

Name of raw materials	INCI	Suppliers of goods
			DendrobiumDendrobium officinale Kimura et Migo	Dendrobium officinale Kimura et Migo	Puer Darun Dendrobium officinale planting Co Ltd
Tremella fuciformis berk	Tremella fuciformis Berk	Kangwang food Co Ltd

The moisturizing facial mask comprises the following components: the tremella-dendrobium extract liquid comprises water, glycerol, 1, 3-propylene glycol, 1, 2-pentanediol, glycerol polyether-26, panthenol, 1, 2-hexanediol and sodium glucoheptonate.

Table 2 plant ingredients total ingredients of moisturizing mask

Raw materials	The addition amount is wt%
		Water (I)	TO100
The invention relates to a tremella-dendrobium extract	40-90
		Glycerol/1, 3-propanediol	0.00-10.00
Glycerol polyether-26/panthenol	0.00-5.00
		1, 2-pentanediol/1, 2-hexanediol	0.05-2.5
Sodium glucoheptonate	0.02-0.10

Example 1

The preparation method of the tremella and dendrobium extract comprises the following steps:

(1) weighing 20 g of tremella and 20 g of dendrobium (tremella: dendrobium = 1: 1);

(2) soaking the tremella and dendrobium mixture in water at the temperature of 20-30 ℃ for 0.5h, and then elutriating;

(3) extraction: adding 2000 mL of water, namely adding the mixture of the materials and the liquid in a ratio of 1: 50, extracting the mixture for 3 hours at the temperature of 115 ℃ under the condition of 70 KPa;

(4) and (3) filtering: filtering the extractive solution with 100 mesh filter cloth; obtaining the tremella and dendrobium extract.

Example 2

(1) Weighing 20 g of tremella and 10 g of dendrobium (tremella: dendrobium = 2: 1);

(3) extraction: adding 2100 mL of water, namely adding the mixture of the materials and the liquid in a ratio of 1:70, extracting the mixture for 3 hours at the temperature of 115 ℃ under the condition of 70 KPa;

(4) and (3) filtering: filtering the extracting solution once by using 200-mesh filter cloth; obtaining the tremella and dendrobium extract.

Example 3

(1) Weighing 20 g of tremella and 5 g of dendrobe (tremella: dendrobe = 4: 1);

(3) extraction: adding 720 mL of water, namely adding the mixture of the materials and the liquid in a ratio of 1:30, extracting the mixture for 3 hours at the temperature of 115 ℃ under the condition of 70 KPa;

Example 4

(1) Weighing 20 g of tremella and 4 g of dendrobium (tremella: dendrobium = 5: 1);

(2) soaking the tremella and dendrobium mixture in water at the temperature of 20-30 ℃ for 1.5h, and then elutriating;

(3) extraction: adding 1250 mL of water, namely adding the mixture of the feed liquid and the mixture of the feed liquid in a ratio of 1: 50, extracting the mixture for 4 hours at the temperature of 125 ℃ and under the pressure of 130 KPa;

Example 5

(3) extraction: adding 2000 mL of water, namely adding the mixture of the materials and the liquid in a ratio of 1: 50, extracting the mixture for 1h at the temperature of 110 ℃ under the condition of 50 KPa;

Example 6

(3) extraction: adding 2000 mL of water, namely adding the mixture of the feed liquid and the mixture in a ratio of 1: 50, extracting the mixture for 3 hours at the temperature of 105 ℃ under the condition of 40 KPa;

Comparative example 1

(1) Weighing 5 g of tremella and 20 g of dendrobium (tremella: dendrobium = 1: 4);

(3) extraction: adding 1250 mL of water, namely adding the mixture of the materials and the liquid in a ratio of 1: 50, mixing the mixture at 70 KPa and 115 deg.CIs/are as followsExtracting for 3 h under the condition;

Comparative example 2

(1) Weighing 20 g of tremella and 2.5 g of dendrobium (tremella: dendrobium = 8: 1);

(3) extraction: 1125 mL of water are added, namely the ratio of the feed to the liquid is 1: 50, extracting the mixture for 3 hours at the temperature of 115 ℃ under the condition of 70 KPa;

Comparative example 3

The same procedure as in example 1 was repeated except that dendrobium was not added to obtain a tremella extract.

(1) Weighing 20 g of tremella;

(2) soaking tremella in water at 20-30 ℃ for 0.5h, and then elutriating;

(3) extraction: adding 1000 mL of water, namely adding the mixture of the materials and the liquid in a ratio of 1: 50, extracting the mixture for 3 hours at the temperature of 115 ℃ under the condition of 70 KPa;

(4) and (3) filtering: filtering the extractive solution with 100 mesh filter cloth; obtaining the tremella extract.

Comparative example 4

The same procedure as in example 1 was repeated except that no white fungus was added to obtain a dendrobe extract.

(1) Weighing 20 g of dendrobe;

(2) soaking the dendrobium in water at the temperature of 20-30 ℃ for 0.5h, and then elutriating;

(4) and (3) filtering: filtering the extracting solution once by using 100-mesh filter cloth; obtaining the dendrobium extract.

Comparative example 5

The preparation method of the tremella and dendrobium extract by the traditional water extraction method comprises the following steps:

(1) weighing 20 g of tremella and 20 g of dendrobe (tremella: dendrobe = 1: 1);

(3) extraction: adding 2000 mL of water, namely adding the mixture of the materials and the liquid in a ratio of 1: 50, extracting the mixture for 3 hours under the condition of normal pressure and 80 ℃;

(4) and (3) filtering: filtering the extractive solution with 100 mesh filter cloth; obtaining the tremella and dendrobium extract by the traditional water extraction method.

Comparative example 6

The traditional water extraction method for preparing the dendrobium extract comprises the following steps:

(1) weighing 20 g of dendrobe;

(3) extraction: adding 1000 mL of water, namely adding the mixture of the materials and the liquid in a ratio of 1: 50, extracting the mixture for 3 hours under the condition of normal pressure and 80 ℃;

(4) and (3) filtering: filtering the extracting solution once by using 100-mesh filter cloth; obtaining the dendrobium extract by the traditional water extraction method.

Tremella dendrobium extract efficacy evaluation

1. Skin feel test of tremella and dendrobium extract

Skin feel test: 30 women aged 18-40 years and dry skin were recruited and 3

areas

1,2, 3 were drawn on the arm and applied with sample 1, sample 2, sample 3, respectively. Scoring the skin feel after trial, wherein the score of each item is 5 points after full score, and the more satisfied the score is, the higher the score is; except for sticky feel, the lower the score the better.

And (3) testing a sample:

sample 1 the method of example 1 was used to prepare a tremella-dendrobium extract;

sample 2 comparative example 3 high temperature high pressure tremella extract;

sample 3 tremella and dendrobium extract from comparative example 5 by water extraction.

Fig. 1 is a comparison graph of skin feel of a tested sample, and from five dimensions, a tremella extract obtained by the sample 2 under high temperature and high pressure is too thick, has obvious sticky feel, feels sticky and greasy, has poor skin feel and has strong filming feel. Compared with the

samples

2 and 3, the tremella and dendrobium extract obtained by the preparation method disclosed by the invention can obviously improve the sticky feeling of tremella, so that the skin feeling is greatly improved, and the moistening feeling, the absorption speed and the smooth feeling of hands are also improved to different degrees.

Fig. 2 is a skin-feel radar chart of different proportions of dendrobium officinale (samples prepared in example 1, comparative example 1 and comparative example 2), and the skin feel is different according to different addition proportions. Compared with a comparative example, the dendrobium officinale extract prepared by the embodiment of the invention has obvious advantages in the aspects of absorption speed, smooth hand feeling, moistening degree and sticky feeling.

The tremella dendrobium nobile prepared according to the proportion of the invention obviously improves the skin feel of the obtained extract.

2. Experiment for removing free radicals by using tremella and dendrobium extract

The experimental method for scavenging free radicals is as follows: preparation is 7 mmol.L^-1ABTS stock solution and 140 mmol. L^-1Potassium persulfate stock solution. A certain amount of 7 mmol. multidot.L^-1ABTS and 140 mmol. L^-1Mixing potassium persulfate, and standing at room temperature in a dark place for a period of time to form ABTS free radical stock solution. A certain amount of ABTS free radical stock solution is taken and diluted by 50% ethanol, so that the light absorption value of the final test negative control is 0.70 +/-0.02.

Diluting the tremella dendrobium extract prepared in example 1 into different concentration gradients, sequentially adding the test solution and the ABTS free radical stock solution, uniformly mixing by vortex, placing the mixture in an enzyme-linked immunosorbent assay for incubation in the dark for a certain time, measuring the light absorption value of the mixture at the wavelength of 734 nm by using the enzyme-linked immunosorbent assay, and calculating the ABTS free radical clearance. As can be seen from FIG. 3, the Tremella herba Dendrobii extract has high free radical scavenging rate.

Example 7 (aqueous mask film liquid)

Mixing 91.98% of white fungus and dendrobium extract (example 1), 1.00% of panthenol, 3.00% of glycerol, 2.00% of 1, 3-propylene glycol, 0.02% of sodium glucoheptonate, 1.00% of glyceryl polyether-26 and 1.00% (capryl hydroximic acid/1, 2-hexanediol/1, 3-propylene glycol) in sequence and stirring uniformly to obtain the aqueous mask liquid.

Example 8 (aqueous mask liquid)

Mixing 92.47% Tremella herba Dendrobii extractive solution (example 1), 0.50% phenoxyethanol/ethylhexyl glycerol, 3.00% 1, 3-propylene glycol, 0.03% disodium EDTA and 1.00% panthenol in sequence, and stirring to obtain aqua facial mask liquid.

Example 9 (aqueous mask liquid)

92.18% of tremella dendrobium extract (example 1), 0.80% of glycerol caprylate, caprylyl hydroximic acid, 1, 2-pentanediol/1, 2-hexanediol, 3.00% of glycerol, 2.00% of butanediol, 0.02% of Crodaquest A300, 2.00% of EG-1 and the like are sequentially mixed and stirred uniformly to obtain the aqueous mask liquid.

Example 10 (aqueous mask liquid)

Mixing 90.48% of tremella and dendrobium extract (example 1), 1.00% of panthenol, 3.00% of glycerol, 2.00% of 1, 3-propylene glycol, 0.02% of sodium glucoheptonate, 1.00% of glyceryl polyether-26, 2.00% of 1, 2-pentanediol and 0.5% of 1, 2-hexanediol in sequence, and stirring uniformly to obtain the aqueous mask liquid.

Example 11 (facial mask liquid containing 40% Tremella and herba Dendrobii extract)

Mixing 40% of tremella and dendrobium extract (example 1), 2.5% (1, 2-pentanediol/1, 2-hexanediol), 3.00% of glycerol, 2.00% of 1, 3-propanediol, 0.02% of Crodaquest A300, 1.00% of EG-1, 1.00% of panthenol and the balance of deionized water in sequence and stirring uniformly to obtain the aqua mask liquid.

Example 12 (facial mask liquid containing 70% Tremella and herba Dendrobii extract)

The aqueous mask liquid is prepared by mixing and stirring 70% of tremella and dendrobium extract (example 1), 2.5% (1, 2-pentanediol/1, 2-hexanediol), 3.00% of glycerol, 2.00% of 1, 3-propanediol, 0.02% of Crodaquest A300, 1.00% of EG-1, 1.00% of panthenol and the balance of deionized water in sequence.

Example 13 (facial mask liquid containing 90% Tremella and herba Dendrobii extract)

Mixing and stirring 90% of tremella and dendrobium extract (example 1), 2.5% (1, 2-pentanediol/1, 2-hexanediol), 3.00% of glycerol, 2.00% of 1, 3-propanediol, 0.02% of Crodaquest A300, 1.00% of EG-1, 1.00% of panthenol and the balance of deionized water in sequence to obtain the aqua mask liquid.

Comparative example 7 (commercial facial mask liquid)

The commercially available moisturizing mask comprises the following components: water, glycerol, 1, 3-propylene glycol, betaine, p-hydroxyacetophenone, sodium citrate, citric acid, hydroxyethyl cellulose, PEG-40 hydrogenated castor oil, sodium alginate, sodium hyaluronate, ascorbyl glucoside, 1, 2-pentanediol, rye (SECALE CEREALE) seed extract, dipeptide diaminobutyryl benzylamide diacetate, and essence.

Comparative example 8 (Water extraction tremella dendrobium facial mask liquid)

The same as in example 10 was repeated except that the Tremella-dendrobium extract of the present invention was replaced with the Tremella-dendrobium extract of the present invention (comparative example 5).

Evaluation of efficacy of aqueous mask film

3. Moisturizing effect test

Selecting 30 volunteers, smearing the test sample on forearm flexor, and testing the water content of tested part at each time point of 15min, 30min, 45min, 1h, 1.5h, 2h, etc. before use.

TABLE 390% influence of Tremella herba Dendrobii extract (example 1) and facial mask solution containing 90% Tremella herba Dendrobii extract (example 13) on skin Water-holding Property

Product(s)	Initial value	After 15min	After 30min	After 45min	After 1h of use	After 1.5h of use	After 2 hours of use
								90% Tremella dendrobium extract (example 1)	24.34	29.67	27.14	25.98	26.15	26.81	25.18
Mask solution containing 90% of tremella and dendrobium extract (example 12)	22.58	33.50	30.48	30.43	32.49	32.11	31.00

As can be seen from Table 3 and FIG. 4, the moisture content of skin can be significantly improved in both the Tremella dendrobium extract and the mask liquid product containing the Tremella dendrobium extract.

4. Skin elasticity test

Selecting 30 volunteers, smearing test samples on the bent side of the forearm, and testing the elasticity of the tested part at several time points, such as 15min, 1h, 2h and the like before and after the use of the 90% dendrobium-tremella extract and the facial mask liquid containing the 90% dendrobium-tremella extract.

TABLE 490% Tremella herba Dendrobii extractive solution (example 1) and facial mask solution containing 90% Tremella herba Dendrobii extractive solution (example 13) for improving skin elasticity

Elasticity of skin	Initial value	After 15min	After 1h of use	After 2 hours of use
					90% Tremella dendrobium extract (example 1)	0.816	1.055	1.110	1.089
Mask liquid containing 90% tremella and dendrobium extract (example 13)	0.814	1.092	1.120	1.120

As can be seen from Table 4 and FIG. 5, both the Tremella herba Dendrobii extract and the mask liquid containing the Tremella herba Dendrobii extract can significantly improve skin elasticity.

5. Skin gloss test

Selecting 30 volunteers, smearing test samples on the bent side of the forearm, and testing the glossiness of the tested part at several time points, such as 15min, 1h, 2h and the like before and after the use of the 90% dendrobium and tremella extract and the facial mask liquid containing the 90% dendrobium and tremella extract.

Influence of surface 590% Tremella herba Dendrobii extract (example 1) and facial mask liquid containing 90% Tremella herba Dendrobii extract (example 13) on skin glossiness

Skin gloss	Initial value	After 15min	After 1h of use	After 2 hours of use
					90% Tremella herba Dendrobii extract (example 1)	3.85	4.14	4.02	4.49
Mask liquid containing 90% tremella and dendrobium extract (example 12)	3.82	4.28	4.34	4.22

As can be seen from Table 5 and FIG. 6, both the Tremella herba Dendrobii extract and the mask liquid containing the Tremella herba Dendrobii extract can significantly improve skin elasticity.

6. Skin feel test of water aqua face mask

Selecting 23 volunteers, applying the water aqua facial mask of the invention for 15 minutes after normal face cleaning, filling in questionnaire survey after cleaning, wherein each total score is 5, the higher the score is, the higher the degree is, and according to trial feedback, finishing the initial feeling evaluation and the skin feeling evaluation of the test subjects on the water aqua facial mask.

TABLE 6 initial feeling evaluation results of aqueous mask-pasting mask liquid

Item	Mean value of	1	2	3	4	5
							Degree of impregnation of mask cloth	4.87	0	0	0	3(13.04%)	20(86.96%)
Degree of uniformity of liquid on membrane cloth	4.61	0	0	2(8.7%)	5(21.74%)	16(69.57%)
							Consistency of mask liquid	3.48	0	4(17.39%)	7(30.43%)	9(39.13%)	3(13.04%)
Color preference of facial mask liquid	3.78	0	3(13.04%)	5(21.74%)	9(39.13%)	6(26.09%)
							Degree of preference for odor of facial mask liquid	3.43	0	4(17.39%)	8(34.78%)	8(34.78%)	3(13.04%)

As can be seen from Table 6 and FIG. 7, the degree of impregnation of the face film cloth and the degree of uniformity of the liquid on the film cloth were highly acceptable.

TABLE 7 evaluation results of skin feel of the aqueous patch

Topic \ options	Average score	1	2	3	4	5
							Moistening sensation	4.17	0	0	3(13.04%)	13(56.52%)	7(30.43%)
Moisturizing effect	4	0	0	4(17.39%)	15(65.22%)	4(17.39%)
							Slippery feel	3.87	0	1(4.35%)	5(21.74%)	13(56.52%)	4(17.39%)
Absorption rate	3.74	0	2(8.7%)	5(21.74%)	13(56.52%)	3(13.04%)
							Sticky feeling	2.7	2(8.7%)	8(34.78%)	8(34.78%)	5(21.74%)	0
Feeling of unity	4.09	0	0	1(4.35%)	82.61%	3(13.04%)

As can be seen from table 7 and fig. 8, the subjects had high evaluations as to the overall moist feeling, moisturizing effect, smoothness, absorption speed, and overall feeling of the mask.

7. Evaluation of effects such as degree of fit and comfort of mask cloth

Three types of common mask cloth are respectively selected, water and water extracted tremella dendrobe mask liquid which are the same in amount as the mask liquid (25 g) of a commercially available mask and the tremella dendrobe mask liquid of the invention are used for soaking the same mask cloth for the same time (30 min), then 6 professional cosmetic formulators perform skin feeling evaluation, 5 is an upper limit, the fitting degree of the mask cloth is increased along with the increase of the score, the comfort degree of the mask cloth is increased, the softness of the mask cloth is increased, and the average value of the trial is taken as the final result for representation. By combining tables 8-10 and trial use of fig. 9-11, it can be seen very intuitively that the tremella-dendrobium mask liquid of the present invention can significantly improve the degree of fit of the mask cloth, and significantly improve the comfort and softness of the mask cloth.

FIGS. 9-11 Picture volunteers used facial mask fluids of Zuoyishu, Zuodi commercially available facial mask fluid, Zuosan water-extracted facial mask fluid containing Tremella and herba Dendrobii (comparative example 8), and Zuosi facial mask fluid containing Tremella and herba Dendrobii (example 10)

TABLE 8 correspondence between cotton cloth and water, commercially available mask solution, aqueous Tremella-Dendrobium mask solution (comparative example 8), and Tremella-Dendrobium mask solution of the present invention (example 10)

Cotton cloth	Water (I)	Commercially available facial mask liquid	Water-extraction tremella and dendrobium facial mask liquid	The invention relates to a tremella and dendrobium facial mask liquid
					Degree of film cloth fit	2.2	3.6	3.2	4.7
Comfort of membrane cloth	2.6	3.8	3.8	4.8
					Softness	2.9	3.9	3.3	4.5

TABLE 9 correspondence between flannelette and water, commercially available facial mask solution, water-extracted Tremella-Dendrobium facial mask solution (comparative example 8) and Tremella-Dendrobium facial mask solution of the present invention (example 10)

Flannelette	Water (W)	Commercially available facial mask liquid	Water-extraction tremella and dendrobium facial mask liquid	The invention relates to a tremella and dendrobium facial mask liquid
					Degree of film cloth fit	2.8	3.4	3.1	4.8
Comfort of membrane cloth	3.2	3.5	3.3	4.4
					Softness	3.5	3.8	3.8	4.7

TABLE 10 correspondences between cuprammonium gauze and water, commercially available facial mask solutions, aqueous tremella and dendrobe facial mask solutions (comparative example 8) and tremella and dendrobe facial mask solutions of the present invention (example 10)

Copper ammonia wire	Water (I)	Commercially available facial mask liquid	Water-extraction tremella and dendrobium facial mask liquid	The invention relates to a tremella and dendrobium facial mask liquid
					Degree of film cloth fit	2.1	3.6	3.3	4.5
Comfort of membrane cloth	2.3	3.2	3.4	4.3
					Softness	1.8	3.4	3.3	4.1

8. Consistency comparison of facial mask solutions

The lower graph shows the consistency comparison of the four facial mask solutions (generally, the greater the consistency, the less the facial mask cloth is wetted, and the best the water penetration should be). As shown in fig. 12, the comparative results of the thickness of the facial mask liquid are: the tremella and dendrobium facial mask liquid is more than a commercially available facial mask liquid and more than a water-extracted tremella and dendrobium facial mask liquid.

9. Influence of different proportions of tremella and dendrobium facial mask liquid on facial mask cloth skin feeling

The superfine cotton mask cloth is soaked by three tremella dendrobium mask solutions with the proportion of 40%, 70% and 90% of the tremella dendrobium extract, skin feeling evaluation is carried out by 6 professional cosmetic formulators, 5 is an upper limit, the moistening feeling is increased, the slippery feeling is increased, the absorption speed is accelerated, the sticky feeling is increased, the overall feeling is better, and the average value of trial use is taken as the final result for presentation. As shown in Table 11, the facial mask solution containing 90% of the Tremella herba Dendrobii extract had excellent moistening and slipping feeling, and also had better overall feeling.

TABLE 11 influence of facial mask liquid of Tremella herba Dendrobii extractive solutions of different proportions on facial mask cloth skin feeling

Superfine cotton	Mask liquid of 40% tremella and dendrobium extract (example 11)	Mask liquid of 70% tremella and dendrobium extract (example 12)	Facial mask liquid of 90% tremella and dendrobium extract (example 13)
				Sense of moistening	2.3	3.4	4.6
Slippery feel	3.2	3.8	4.7
				Absorption rate	4.1	3.8	3.5
Sticky feeling	1.8	2.5	2.8
				Feeling of unity	2.9	3.4	4.2

The inventors test the yield and molecular weight of the dendrobium candidum extract polysaccharide of the white fungus, and the test process and results are as follows:

polysaccharide yield test

The test method comprises the following steps: polysaccharide determination is carried out by adopting a phenol-sulfuric acid method (specifically, refer to the determination of polysaccharide content in tremella [ J ]. Heilongjiang pharmaceutical science, 1999(06): 32-32.).

Test samples: example a tremella dendrobium extract was prepared.

And (3) testing results: the polysaccharide content is 1.20 mg/mL-4.24 mg/mL.

Sample(s)

Example 1

Example 2

Example 3

Example 4

Example 5

Example 6

Polysaccharide content

4.24mg/mL

3.09mg/mL

2.94mg/mL

1.20mg/mL

3.20mg/mL

3.04mg/mL

The tremella-dendrobium extract prepared in example 1 is the highest polysaccharide content, and the polysaccharide content is 4.24 mg/mL.

Determination of molecular weight of tremella and dendrobium extract polysaccharide

Determination of molecular weight distribution: the detection method adopts high performance gel permeation chromatography (specifically referring to high performance gel permeation chromatography-based tremella polysaccharide quality control research, J Chinese herbal medicine, 2011, 42 (09): 1732-1735.).

The molecular weight distributions of the dendrobium candidum polysaccharide prepared in example 1, the dendrobium polysaccharide in comparative example 4 and the tremella polysaccharide in comparative example 3 are shown in fig. 13, fig. 14 and fig. 15.

As can be seen from fig. 13, 14 and 15, the molecular weight of the dendrobium polysaccharide is smaller, the molecular weight of the tremella polysaccharide is larger, the distribution of the molecular weight of the dendrobium polysaccharide prepared in example 1 in the invention is greatly changed compared with that of the dendrobium extract and the tremella extract, and the molecular weight distribution range is from 400 Da to 580 ten thousand Da. The molecular distribution of the dendrobium polysaccharide is mainly 300 Da to 260 ten thousand Da.

The above experiments show that the molecular weight distribution of the tremella-dendrobium extract is improved under the preparation process conditions of the invention.

Claims

1. An aqueous face mask is characterized in that: the raw materials comprise the following components in percentage by weight: 82.4% -99.3% of tremella-dendrobium extract, 0.00% -10.00% of humectant, 0.05% -2.5% of preservative, 0.02% -0.10% of chelating agent and 0.00% -5.00% of skin feel regulator; the tremella-dendrobium extract is prepared by compounding tremella and dendrobium according to a certain proportion and adopting a high-temperature high-pressure preparation process.

2. The aqueous face mask of claim 1, wherein: the preparation method of the tremella and dendrobium extract specifically comprises the following steps:

3. The aqueous face mask according to claim 1 or 2, characterized in that: the weight ratio of tremella to dendrobium in the tremella-dendrobium extract is 1: 1-5: 1.

4. An aqueous facial mask according to any of claims 1 to 3, characterized in that: in the step of extracting the tremella and dendrobium extract, the extraction pressure is 40-75 KPa; the extraction temperature is 105-115 ℃; the extraction time is 2-3 h.

5. The aqueous face mask according to any one of claims 1 to 4, characterized in that: in the step of extracting the tremella and dendrobium extract, the extraction pressure is 70 KPa; the extraction temperature is 115 ℃; the extraction time is 3 h.

6. The aqueous face mask according to any one of claims 1 to 5, characterized in that: in the step of extracting the tremella-dendrobium extract, the ratio of the tremella-dendrobium to water is 1: 30-1: 70.

7. The aqueous face mask according to any one of claims 1 to 6, characterized in that: the humectant is one or more of glycerol, 1, 3-propylene glycol and polyalcohol.

8. The aqueous face mask according to any one of claims 1 to 7, wherein: the preservative is one or more of the conventional preservatives in the field.

9. The aqueous face mask according to any one of claims 1 to 8, wherein: the chelating agent is one or more of sodium glucoheptonate, EDTA disodium, EDTA trisodium and EDTA tetrasodium.

10. The aqueous face mask of any one of claims 1-9, wherein: the skin feeling regulator is one or more of glyceryl polyether-26, panthenol and other aqueous skin feeling regulators.