CN114081853B

CN114081853B - Plant composition and preparation method and application thereof

Info

Publication number: CN114081853B
Application number: CN202110325667.9A
Authority: CN
Inventors: 孟宏; 曲召辉
Original assignee: Taihe Kangmei Beijing Research Institute of Traditional Chinese Medicine Co Ltd
Current assignee: Taihe Kangmei Beijing Research Institute of Traditional Chinese Medicine Co Ltd
Priority date: 2021-03-26
Filing date: 2021-03-26
Publication date: 2023-08-04
Anticipated expiration: 2041-03-26
Also published as: CN114081853A

Abstract

The invention discloses a plant composition, a preparation method and application thereof, and belongs to the field of daily cosmetics. The plant composition comprises tremella 10-30g, dendrobium 10-30g, ganoderma lucidum 2-6g and saffron 0.001-0.3g. The invention uses the medical and edible ideas to complement each other and scientifically combine the medical and edible ideas from the aspect of traditional Chinese medicine beauty treatment to form the plant composition with multiple effects and obvious effects. The plant composition has the effects of comfort skin feel, moisturizing, water locking, improving skin elasticity, reducing canthus fine wrinkles, preventing canthus fine wrinkles from growing and the like, can be directly applied to skin care products as natural plant raw materials, can also be directly used as a matrix for cosmetics such as toning lotion, emulsion, cream, facial mask and the like, and has wide market application prospect.

Description

Plant composition and preparation method and application thereof

Technical Field

The invention relates to the field of daily cosmetics, in particular to a plant composition and a preparation method and application thereof.

Background

The modern skin has various problems such as dryness, lack of water, loss of elasticity, dark yellow complexion, large pores, sensitive skinning and the like caused by the living conditions such as fast living rhythm, high working pressure, insufficient sleep, lack of exercise and the like. Moisture in skin is an important factor for moistening skin, keeping skin healthy and preventing skin aging, and effective moisturizing nursing can delay skin aging, improve skin tolerance to external stimulus and prevent skin diseases. At present, most of the commercial cosmetics can alleviate the problem of skin dryness to a certain extent, but cannot meet the skin care requirement of consumers for comprehensively improving the skin state from the aspect of skin health care. In recent years, with the progress of the times and the increase of people's chong and returning to natural demands, various traditional Chinese medicine effect cosmetics produced by using traditional Chinese medicine extracts as cosmetic additives are increasingly sought by consumers.

The patent (CN 201910649489.8) discloses a facial mask containing tremella and dendrobium extracts and a preparation method thereof, wherein the tremella and dendrobium extracts with extremely high polysaccharide content are selected, other skin-feel modifiers and other complicated skin-feel conditioning agents are not added, and the facial mask is simple in components, natural, smooth and super-strong in moisturizing and repairing. However, only the effects of moisturizing and moisturizing the mask are considered, and the skin care requirements of consumers on comprehensively improving the skin state are ignored.

Based on this, it is necessary to develop a plant composition having a pleasant skin feel, moisturizing, improving skin elasticity, reducing fine wrinkles on the corners of the eyes, and preventing the growth of fine wrinkles on the corners of the eyes by means of scientific compatibility.

Disclosure of Invention

Based on the defects in the prior art, the invention provides a plant composition which has the effects of comfort skin feel, moisturizing, water locking, improving skin elasticity, reducing fine wrinkles of the corners of the eyes, preventing the fine wrinkles of the corners of the eyes from growing and the like; the plant composition can be used as natural plant material for directly applying to skin care product, and can be directly used as matrix for cosmetic such as astringent, emulsion, cream, facial mask, etc.

The technical problems to be solved by the invention are realized by the following technical scheme:

according to the invention, the plant composition comprises tremella, dendrobium, ganoderma lucidum and saffron.

According to some specific embodiments of the present invention, the botanical composition comprises the following components in parts by weight: 10-30g of tremella, 10-30g of dendrobium, 2-6g of lucid ganoderma and 0.001-0.3g of saffron.

According to some specific embodiments of the present invention, the botanical composition comprises the following components in parts by weight: 10-30g of tremella, 10-30g of dendrobium, 2-6g of lucid ganoderma and 0.001-0.01g of saffron.

According to the present invention, the plant composition is prepared by a preparation method comprising:

1) Soaking and washing tremella and dendrobium;

2) Loading Ganoderma and stigma croci Sativi into filter bag, and mixing with elutriated Tremella and herba Dendrobii;

3) Extracting the plant raw materials in the step 1) and the step 2) with water according to a certain feed liquid ratio under the conditions of high temperature and high pressure;

4) Filtering and sterilizing.

According to the invention, the preparation pressure in the preparation method is 0.035-0.055MPa.

According to the invention, the preparation temperature in the preparation method is 100-130 ℃.

According to the invention, the treatment time in the preparation method is 1.0-4.0. 4.0 h.

According to some embodiments of the invention, the preparation pressure in the preparation method is 0.04MPa.

According to some embodiments of the invention, the preparation temperature in the preparation method is 110 ℃.

According to some embodiments of the invention, the processing time in the preparation method is 2.0. 2.0 h.

According to the invention, the feed liquid ratio of the plant raw material to water in the preparation method is 1:30-1:70 (m/m).

According to some embodiments of the invention, the plant material to water feed ratio in the preparation method is 1:45 (m/m).

According to the invention, the tremella is dry tremella, and the tremella with large blocks is torn into small blocks of 1-3 cm.

According to the invention, the filter bag is a non-woven filter bag.

According to the invention, the extract is filtered through a 100-200 mesh filter cloth.

In a second aspect, the present invention provides a method for preparing a plant composition according to the first aspect of the invention.

According to the invention, the preparation method comprises the following steps:

1) Weighing 10-30g of tremella and 10-30g of dendrobium, soaking and washing;

2) Weighing 2-6g of ganoderma lucidum and 0.001-0.3g of saffron, filling the ganoderma lucidum and the saffron into a filter bag, and mixing the ganoderma lucidum with the elutriated tremella and dendrobium;

3) Extracting the plant raw materials in the step 1) and the step 2) with water for 1.0-4.0h under the conditions of high temperature and high pressure with the preparation pressure of 0.035-0.055MPa and the preparation temperature of 100-130 ℃ according to the feed liquid ratio of 1:30-1:70 (m/m);

4) Filtering and sterilizing.

According to the invention, the filter bag is a non-woven filter bag.

In a third aspect, the present invention provides the use of a plant composition according to the first aspect of the present invention or a plant composition according to the second aspect of the present invention in cosmetics, wherein the plant composition can be directly used as a base material in cosmetics such as lotions, emulsions, creams, masks and/or skin care products.

According to the present invention, the botanical composition may be used directly as a base in cosmetics that use good skin feel and/or moisturize and/or lock water and/or improve skin elasticity and/or reduce periocular fine wrinkles and/or prevent periocular fine wrinkles from growing.

According to the present invention, the kind and formulation of the cosmetic are not particularly limited, such as a mask, cream, facial cleanser, essence, emulsion, etc.

The invention has the beneficial effects that:

1. the invention uses the medical and edible ideas from the beauty treatment of traditional Chinese medicine, takes the effects of supplementing liquid and promoting the production of body fluid of dendrobium candidum, takes the effects of resisting allergy and diminishing inflammation of lucid ganoderma, takes the effects of reinforcing water and protecting screen of tremella, captures the benefits of nourishing yin and moistening and nourishing saffron, and uses the scientific compatibility to complement each other to realize the best synergistic effect, thus forming the plant composition with the effects of supplementing water, preserving moisture, locking water, improving skin elasticity, reducing periocular fine wrinkles, preventing the growth of canthus fine wrinkles and the like.

2. The plant composition of the invention adopts natural plants, has simple components and meets the requirements of modern people on natural plant raw materials.

3. The plant composition can be directly applied to skin care products as a natural plant raw material, has comfortable skin feel and excellent moisturizing effect, can be directly used as a matrix in cosmetics such as toning lotion, emulsion, cream, facial mask and the like, and has wide market application prospect.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are required to be used will be briefly described below.

FIG. 1 is a skin feel test chart of example 1, example 5 and comparative examples 2 to 9;

FIG. 2 is a graph of the results of skin moisture content tests for example 1, example 5 and comparative examples 4-9;

FIG. 3 is a graph of the results of the canthus fine line test of example 1, example 5 and comparative examples 4-9;

FIG. 4 is a graph of skin moisture content test results for a mask;

FIG. 5 is a graph of the skin elasticity R2 test results of the mask;

FIG. 6 is a graph of the skin elasticity R5 test results of the mask;

FIG. 7 is a graph of the skin elasticity R7 test results of the mask;

fig. 8 is a graph of the results of the canthus fine line test of the mask.

Detailed Description

The invention will be further illustrated with reference to specific examples, but the invention is not limited thereto.

The experimental methods described in the following examples are all conventional methods unless otherwise specified; the experimental materials and reagents, unless otherwise specified, are commercially available.

The main raw material names, suppliers/manufacturers and places of production of the invention are shown in Table 1:

TABLE 1 raw materials and sources

Raw material name	SupplyingCommercial/manufacturer	Production area
			DendrobiumDendrobium officinale Kimura et Migo	Pu' er reach Dendrobium candidum planting Co.Ltd	Yunnan (Yunnan) province
Tremella fuciformis berk	Kangwang food Co., ltd	Fujian (Fujian)
			Ganoderma lucidum	Beijing Qianzao Chinese herbal pieces Co., ltd	Beijing
Saffron crocus flower	Beijing Qianzao Chinese herbal pieces Co., ltd	Beijing
			Hexanediol (Hexadiol)	De Xin (Shanghai) Limited	Germany
Pentanediol	De Xin (Shanghai) Limited	Germany

Example 1

Weighing 20g of tremella, 20g of dendrobium, 4g of lucid ganoderma and 0.01g of saffron; firstly, soaking and washing tremella and dendrobium; loading Ganoderma and stigma croci Sativi into filter bag, mixing with elutriated Tremella and herba Dendrobii, adding 1980mL water, extracting with feed liquid ratio of 1:45 (m/m) under 0.04MPa at 110deg.C for 2.0 h; filtering the extractive solution with 200 mesh filter cloth for one time to obtain plant composition.

Example 2

Weighing 10g of tremella, 10g of dendrobium, 2g of ganoderma lucidum and 0.001-g of saffron; firstly, soaking and washing tremella and dendrobium; loading Ganoderma and stigma croci Sativi into filter bag, mixing with elutriated Tremella and herba Dendrobii, adding 1540mL water, extracting at 100 deg.C under 0.035MPa and 100 deg.C at 1:70 (m/m) of feed-liquid ratio to 2.0 h; filtering the extract with 200 mesh filter cloth; obtaining the plant composition.

Example 3

Weighing 30g of tremella, 30g of dendrobium, 6g of ganoderma lucidum and 0.3g of saffron; firstly, soaking and washing tremella and dendrobium; loading Ganoderma and stigma croci Sativi into filter bag, mixing with elutriated Tremella and herba Dendrobii, adding 1980mL water, extracting at 130 deg.C under 0.055MPa and 1.0. 1.0 h with feed-liquid ratio of 1:30 (m/m); filtering the extract with 200 mesh filter cloth; obtaining the plant composition.

Example 4

Weighing 20g of tremella, 20g of dendrobium, 4g of lucid ganoderma and 0.01g of saffron; firstly, soaking and washing tremella and dendrobium; loading Ganoderma and stigma croci Sativi into filter bag, mixing with elutriated Tremella and herba Dendrobii, adding 1980mL water, extracting at 130 deg.C under 0.055MPa and 1:45 (m/m) of feed-liquid ratio to 2.0 h; filtering the extractive solution with 200 mesh filter cloth for one time to obtain plant composition.

Example 5

Weighing 20g of tremella, 20g of dendrobium, 4g of lucid ganoderma and 0.01g of saffron; firstly, soaking and washing tremella and dendrobium; loading Ganoderma and stigma croci Sativi into filter bag, mixing with elutriated Tremella and herba Dendrobii, adding 1980mL water, extracting at 100 deg.C under 0.035MPa and 1:45 (m/m) of feed-liquid ratio of 2.0 h; filtering the extractive solution with 200 mesh filter cloth for one time to obtain plant composition.

Comparative example 1

Weighing 20g of tremella, 20g of dendrobium, 4g of lucid ganoderma and 0.01g of saffron; firstly, soaking and washing tremella and dendrobium; loading Ganoderma and stigma croci Sativi into filter bag, mixing with elutriated Tremella and herba Dendrobii, adding 1980 and mL water, extracting at 85deg.C under normal pressure of 2.0 h at a feed-liquid ratio of 1:45 (m/m); filtering the extractive solution with 200 mesh filter cloth for one time to obtain plant composition.

Comparative example 2

Weighing 20g of dendrobium, 4g of lucid ganoderma and 0.01g of saffron; firstly, soaking and washing dendrobium; loading Ganoderma and stigma croci Sativi into filter bag, mixing with herba Dendrobii, adding 1080mL water with a feed-liquid ratio of 1:45 (m/m), and extracting at 110 deg.C under 0.04MPa for preparation pressure of 2.0 h; filtering the extractive solution with 200 mesh filter cloth for one time to obtain plant composition.

Comparative example 3

Weighing 20g of tremella, 4g of ganoderma lucidum and 0.01g of saffron; firstly, soaking and washing tremella; loading Ganoderma and stigma croci Sativi into filter bag, mixing with elutriated Tremella, adding 1080mL water with a feed-liquid ratio of 1:45 (m/m), and extracting at 110 deg.C under 0.04MPa for preparation pressure of 2.0 h; filtering the extractive solution with 200 mesh filter cloth for one time to obtain plant composition.

Comparative example 4

Weighing 20g of tremella, 20g of dendrobium and 4g of lucid ganoderma, and firstly soaking and washing tremella and dendrobium; loading Ganoderma into filter bag, mixing with elutriated Tremella and herba Dendrobii, adding 1980mL water with a feed-liquid ratio of 1:45 (m/m), extracting under 0.04MPa at 110deg.C for 2.0 h; filtering the extractive solution with 200 mesh filter cloth for one time to obtain plant composition.

Comparative example 5

Weighing 20g of tremella, 20g of dendrobium nobile and 0.01g of saffron, firstly soaking and washing tremella and dendrobium nobile; charging saffron into filter bag, mixing with elutriated tremella and dendrobium, charging 1800mL water, extracting 2.0. 2.0 h under the conditions of preparation pressure 0.04MPa and preparation temperature 110 deg.C, the feed-liquid ratio 1:45 (m/m); filtering the extractive solution with 200 mesh filter cloth for one time to obtain plant composition.

Comparative example 6

Weighing 20g of tremella and 20g of dendrobium; soaking and washing tremella and dendrobium; 1800mL of water is added, the feed-liquid ratio is 1:45 (m/m), and the extraction is 2.0 h under the conditions of the preparation pressure of 0.04MPa and the preparation temperature of 110 ℃; filtering the extract with 200 mesh filter cloth for one time to obtain Tremella herba Dendrobii composition.

Comparative example 7

Weighing 20g of tremella, 20g of dendrobium, 4g of lucid ganoderma and 0.01g of saffron; firstly, soaking and washing tremella and dendrobium; loading Ganoderma and stigma croci Sativi into filter bag, mixing with elutriated Tremella and herba Dendrobii, adding 1980mL water, extracting at 100 deg.C under 0.02 MPa and 1:45 (m/m) of feed-liquid ratio to 2.0 h; filtering the extractive solution with 200 mesh filter cloth for one time to obtain plant composition.

Comparative example 8

Weighing 20g of tremella, 20g of dendrobium, 4g of lucid ganoderma and 0.01g of saffron; firstly, soaking and washing tremella and dendrobium; loading Ganoderma and stigma croci Sativi into filter bag, mixing with elutriated Tremella and herba Dendrobii, adding 1980mL water, extracting with feed liquid ratio of 1:45 (m/m) under 0.06 MPa at 135 deg.C for 2.0 h; filtering the extractive solution with 200 mesh filter cloth for one time to obtain plant composition.

Comparative example 9

Weighing 6g of ganoderma lucidum, loading into a filter bag, adding 270mL of water, extracting 2.0. 2.0 h under the conditions of preparation pressure of 0.04MPa and preparation temperature of 110 ℃ in a feed-liquid ratio of 1:45 (m/m); filtering the extractive solution with 200 mesh filter cloth for one time to obtain Ganoderma extract.

Comparative example 10

Weighing 0.3g of saffron, loading into a filter bag, mixing with elutriated tremella and dendrobium nobile, adding 13.5mL of water, extracting 2.0. 2.0 h under the conditions of preparation pressure of 0.04MPa and preparation temperature of 110 ℃ with a feed-liquid ratio of 1:45 (m/m); filtering the extractive solution with 200 mesh filter cloth for one time to obtain stigma croci Sativi extract.

Comparative example 11

Weighing 5g of tremella, 20g of dendrobium, 20g of lucid ganoderma and 0.01g of saffron; firstly, soaking and washing tremella and dendrobium; loading Ganoderma and stigma croci Sativi into filter bag, mixing with elutriated Tremella and herba Dendrobii, adding 2025mL water, extracting with feed liquid ratio of 1:45 (m/m) under 0.04MPa at 110deg.C for 2.0 h; filtering the extractive solution with 200 mesh filter cloth for one time to obtain plant composition.

Comparative example 12

Weighing 20g of tremella, 5g of dendrobium, 20g of lucid ganoderma and 0.01g of saffron; firstly, soaking and washing tremella and dendrobium; loading Ganoderma and stigma croci Sativi into filter bag, mixing with elutriated Tremella and herba Dendrobii, adding 2025mL water, extracting with feed liquid ratio of 1:45 (m/m) under 0.04MPa at 110deg.C for 2.0 h; filtering the extractive solution with 200 mesh filter cloth for one time to obtain plant composition.

Basic index test of plant compositions with different preparation processes

1.1 polysaccharide and Total sugar content determination

The method is carried out by phenol-sulfuric acid method (specifically referring to the content determination of polysaccharide in tremella [ J ]. Heilongjiang medical science, 1999 (06): 32-32.).

1.2 pH value: measured using a pH meter.

1.3 Viscosity number: measured by a digital display rotary viscometer.

1.4 Solid content: measured by a solid content meter.

1.5 Skin feel and moisturizing effect: the evaluation method is sensory evaluation of people, and after the smearing sample is tried out, a tester simply describes smearing feeling, including moistening feeling, astringency, sticky feeling and moisturizing effect.

The preparation process has certain influence on the extraction efficiency, efficacy expression and the like of the plant composition. The water extraction method is a preparation method commonly used for plant extracts, and is simple and easy to implement. The plant composition prepared by the water extraction method is used as a control, and the influence of the extraction process on the basic properties and the efficacy of the plant composition is compared.

Table 2 index data for comparative example 1 and example 1

As shown in Table 2, the conventional water extraction method has low extraction efficiency, the polysaccharide content and the total sugar content in the plant composition are low, and the extraction of active substances is incomplete. If the extraction efficiency of the water extraction method is to be improved, the raw materials are required to be crushed in advance, and the crushing can cause filtering difficulty while greatly aggravating the manpower, material resources and time of the experiment, and the operation steps of early standing, suction filtration and the like are required to be added, so that the experimental time is doubled. The high-temperature high-pressure method is a method for extracting active ingredients under a high-pressure airtight condition, the extraction method can accelerate the reaction during extraction, and meanwhile, the airtight condition can enable water vapor to fully contact each medicinal material, so that the reaction efficiency is improved.

As can be seen from Table 2, compared with comparative example 1, the high temperature and high pressure process improves the polysaccharide content, total sugar content, viscosity and solid content of the plant composition, improves the extraction rate of active substances in the plant composition, avoids resource waste caused by insufficient raw material utilization, and greatly saves cost. Meanwhile, comparative examples 1 to 5, comparative example 7 and comparative example 8 can find that high temperature and high pressure process conditions are important factors affecting the extraction yield. When the temperature and pressure of the high-temperature high-pressure treatment are lower than a certain range, the extraction rate of the active substances of the plant extract is low, and the polysaccharide content, the total sugar content, the viscosity and the solid content are lower than those of the examples (example 5, comparative example 7), so that weak influence on skin feel may be generated; when the temperature and pressure of the high-temperature high-pressure treatment exceeds a certain range, the higher temperature and pressure basically do not improve the extraction efficiency of plants, such as (example 4 and comparative example 8), and have no influence on skin feel, but the excessively high pressure not only brings potential safety hazard, but also increases energy consumption and generates energy waste.

The tremella is rich in natural colloid, the solution is easy to become thick, when the water extraction method with low temperature and pressure is adopted for low extraction, the natural colloid in the tremella is difficult to extract, and when the water extraction method is adopted for extraction, the texture of the extracting solution is thin, and the extraction rate of active substances is low. Comparative example 1 (water extraction method) has an extract viscosity of only 5.5 mPas and a thin texture; the extract of example 1 (high temperature high pressure method) had a viscosity of 97.9 mPas and a thick texture, similar to a serum. The extract of example 1 (high temperature and high pressure method) has a texture similar to that of essence, skin feel is moist, and after dendrobium is added, the whole astringent feel and sticky feel of the extract of example 1 are reduced. The water extraction method is poor in moisturizing effect of the solution of comparative example 1 (water extraction method) because the extraction efficiency is low and the active ingredients are not completely extracted.

Basic index test of different raw material plant compositions

To test the basic indexes of the plant compositions of different raw materials, the indexes of the examples 1, 4-5, 2-6 and 9-12 such as pH value, viscosity (mPas), solid content (%), skin feel, moisturizing effect and the like were further measured, and the test methods are shown as 1.2-1.5.

TABLE 3 various index data for different raw plant compositions

As shown in table 3, the plant composition components significantly affected the viscosity, skin feel and moisturizing effect of the plant composition. Compared with comparative example 2, example 1 has the characteristics of moist feel, no astringent feel, light sticky feel, better moisturizing effect and the like, and has thick texture like essence; compared with the example 1, the comparative example 2 has small viscosity, which shows that the tremella macromolecular polysaccharide can increase the solution consistency and provide a certain moist skin feel, and the macromolecular polysaccharide can form a layer of protective film on the skin surface, so that the moisturizing effect of the composition is enhanced; in comparative example 11, the amount of tremella is reduced, and the consistency of the solution is changed, so that the moistening feeling is reduced, but the moistening feeling is still obtained; compared with example 1, comparative example 3 has slightly astringent feel, because dendrobe has the effect of lubricating skin, the lack of dendrobe can cause the skin feel to be slightly worse, and the combination of dendrobe and tremella can synergistically increase, so that the skin feel becomes moist and smooth; comparative example 12 reduced the amount of dendrobe used, reduced the oily feel of the solution, and produced some astringent feel; comparative examples 4 to 6 have no significant difference in skin feel from example 1; comparative example 9 glossy ganoderma extract, which has a texture similar to that of water, but slightly sticky; comparative example 10 is saffron extract, texture and water. In conclusion, the plant composition has good skin feel and moisturizing effect by reasonably combining the four plant raw materials of dendrobium, tremella, ganoderma lucidum and saffron and the synergistic effect of the functional components.

Evaluation of plant composition efficacy

To verify the efficacy of the product, and the effect of each ingredient on the product, the relevant efficacy tests were performed by screening examples 1, 5, and 2-9 from the comparative examples in combination with the above data.

3.1 skin feel test

Evaluating the sample: examples 1, 5, comparative examples 2-9;

test population: 45 total (women, ages 18-55, meeting the subject's volunteer inclusion criteria);

the testing method comprises the following steps: the 50 uL evaluation samples were applied to the backs of the left and right hands, respectively, and were softly swirled and applied 10 times with the other hand, and then the consistence, moist feel, absorption speed, astringency and sticky feel of the samples were evaluated in order in a scoring manner (1-5 minutes), and the test results are shown in fig. 1.

Consistency approval: a degree of acceptance of the appearance of the product by the subject is shown, 1 represents no acceptance, 5 represents acceptance;

moisturizing feel: the skin is moist after use, 1 represents very dry, 5 represents very moist;

absorption rate: the absorption speed of the product when being smeared is 1, which is not easy to absorb, 5, which is easy to absorb;

astringent feel: the unsmooth feel of the product when being smeared is 1, which means that the product is smooth, and 5, which means that the unsmooth feel is heavy;

sticky feel: sticky feel of skin after use, 1 represents non-sticky, 5 represents very sticky;

as shown in fig. 1, example 1 had a better moisturization feel, and the subject scores were all above 4 points. Example 5 is not much different from example 1 in skin feel compared to example 1; the moisture effect of the extracting solution of the comparative example 2 is poor, the score of the subjects is below 3 minutes, the sticky feeling and the astringent feeling of the extracting solution of the comparative example 2 are heavy, and the satisfaction degree of most subjects is low, because after the tremella is absent, the extracting solution is free of tremella macromolecular polysaccharide, and the solution is thin and lacks the moisture feeling; the consistency of the extracting solution in the comparative example 3 is approved by most subjects, but the viscosity is too heavy, the skin feel is sticky after the application, and the extracting solution is not easy to absorb; comparative examples 4 to 6 were not very different in skin feel, but the color of the extract was slightly different due to the difference between saffron and ganoderma lucidum itself; comparative example 7 was thinner, lacked a moist feel, and slightly sticky; comparative example 8 skin feel similar to clear water; in comparative example 9, the tremella was reduced in amount, the consistency was slightly thin, and the moist feeling was also reduced.

3.2 moisture content test

Evaluating the sample: examples 1, 5, comparative examples 2-9;

moisture content tests were performed on examples 1, 5, and comparative examples 2 to 9. The moisture retention effect of the sample is measured and evaluated by using a skin moisture content tester Corneometer CM825 after the use for 4 hours through single human trial on the arm, and the effect of improving the skin moisture content of the sample is evaluated and evaluated by comparing the skin moisture content changes before and after the use.

30 volunteers (women, 18-55 years old, and meeting the volunteer selection criteria) were selected for the test, and the samples were evaluated to be randomly distributed on the left and right forearm flexor sides of the volunteers, and the skin moisture contents of the forearm flexor sides before, after 5min, after 1h, after 2h, and after 4h of the test were measured, and the test results are shown in fig. 2.

As shown in fig. 2, the skin moisture content can be significantly improved in each of examples 1, 5 and comparative examples 4 to 6 and 9 for 5 minutes after use, and the skin moisture content is not significantly improved in comparative examples 7 to 8, compared with the initial value (before use); the skin moisture content gradually decreases as the time after use is prolonged. Example 1 and example 5 still gave higher moisture levels in the skin after use of 4h compared to the comparative examples, indicating good moisturization and water retention. And the moisturizing effect of example 1 and example 5 is superior to that of the plant compositions obtained in comparative examples 2 to 6 and the single extracts obtained in comparative examples 9 to 10, which means that the plant composition of the present invention has better moisturizing and water-locking effects than any single extract and the composition prepared by combining any two or three of the raw materials. Experimental data show that the plant composition has better effects of moisturizing and locking water through reasonable proportion of four plant raw materials of dendrobium, tremella, saffron and ganoderma lucidum.

3.3 test for reducing canthus fine wrinkles

Evaluating the sample: examples 1, 5, comparative examples 2-9;

test population: 45 (women, ages 18-55, meeting the subject's volunteer selection criteria);

the testing method comprises the following steps: the right and left corners of eyes are respectively smeared with a proper amount and the same amount of evaluation samples every day, and the volunteers are tested for the fine wrinkles of the corners of eyes 30 minutes, 1 week and 2 weeks before and after using by using an EvaSKIN instrument, and the test results are shown in figure 3.

As can be seen from fig. 3, examples 1, 5 and 9 all had a certain effect of reducing fine wrinkles at the canthus after 30 minutes of use compared with the initial value (before use), and the difference between the remaining samples was small and hardly had an effect. However, in terms of long-term effect, example 1 and example 5 have a remarkable effect of reducing canthus fine wrinkles after 2 weeks of use, as compared with comparative examples 5 and 9, i.e., example 1 and example 5 have effects of reducing canthus fine wrinkles and preventing canthus fine wrinkles from growing for a long period of time. Comparative examples 4, 5, 8 did not significantly reduce the canthus moire effect, but maintained substantially no increase in moire, while comparative examples 6, 7 did not reduce the moire effect. Experimental data further indicate that the botanical compositions of the present invention have better efficacy in reducing canthus fine lines than compositions prepared from any single extract and any combination of two or three of the materials. The plant composition has the effects of reducing the fine wrinkles of the canthus and preventing the fine wrinkles of the canthus from growing for a long time by reasonably proportioning the four plant raw materials of the dendrobium, the tremella, the saffron and the ganoderma lucidum and synergistically increasing.

Mask preparation method

From the experimental results, the plant composition disclosed by the invention has good skin feel and has the effects of moisturizing, reducing fine wrinkles on the corners of eyes and the like. In order to further study the application of the plant composition in the cosmetic field, the mask prepared in the embodiment 1 is used for crowd trial, and the efficacy of the plant composition in the cosmetic application is discussed. The type and formulation of the cosmetic of the present invention are not particularly limited, and any type of cosmetic known in the art may be used, such as a mask, cream, face wash, essence, emulsion, etc.

The mask comprises the following specific raw materials and preparation method:

table 4 mask formulation raw materials and formulation

The preparation method of the mask comprises the following steps:

adding the raw materials into a beaker according to the formula raw materials and the proportion of the mask shown in Table 4, heating for 30min at 95 ℃, cooling, soaking the mask cloth, and finally packaging into a mask bag.

Evaluation of mask Using Effect

The testing method comprises the following steps:

selecting 50 volunteers (female, 18-55 years old, according with the volunteer selection standard of the subject), and dividing the volunteers into 2 groups of 25 people, wherein 1 group of left face uses the mask of example 1, and the right face uses the deionized water mask; the left face of group 2 used deionized water mask and the right face used the mask of example 1.

The back half face of the face is applied at night for 15min, and is used continuously for 2 weeks, and the skin index tests of initial value, 5min, 30min, one week and two weeks, including skin moisture content, skin elasticity R2/R5/R7 value and canthus fine lines, are respectively carried out on volunteers every other day. Wherein, 5min and 30min after use are the instant effect of the mask, and the mask is directly tested without washing the face after use; the long-term effect is achieved after 1 week and 2 weeks, and the test is carried out after the face is washed by clean water and the test is still carried out for 30 minutes. The skin elasticity R2/R5/R7 value is that the total elasticity R2 is, and the closer the value is to 1, the higher the skin elasticity is; r5 is net elasticity, the closer the value is to 1, the higher the skin elasticity; r7 is elastic recovery ability, and the closer the value is to 1, the higher the skin elasticity is.

Skin moisture content was measured using a skin moisture tester Corneometer CM825, skin elasticity was measured using a skin elasticity tester Cutometer MPA580, and canthus fine lines were measured using an EvaSKIN instrument.

As shown in fig. 4, the skin moisture content was immediately raised after using both the deionized water mask and the mask of example 1 of the present invention for 5 minutes, and the skin moisture content was higher after using the mask of example 1, as compared with the initial value (before using). After 30min, the moisture content of the skin of the deionized water mask is rapidly reduced, and the moisture is possibly taken away while the moisture evaporates, so that the facial skin becomes drier like a skin care product after face washing. From the long-term effect, the mask of example 1 gradually increases the skin moisture content after two weeks of use, but the deionized water mask has no effect of increasing the skin moisture content, which indicates that the plant composition of the invention has better moisturizing and water locking effects.

The deionized water mask and the mask of the embodiment 1 of the invention show that the skin elasticity is shown in figures 5-7, and compared with the initial value (before use), the mask of the embodiment 1 of the invention has better improving effect on the R2/R5/R7 values of the skin elasticity immediately and for a long time, and the plant composition of the invention has the effect of improving the skin elasticity.

The number of fine wrinkles at the corners of eyes using the mask of example 1 and the deionized mask of the present invention is shown in fig. 8, and the number of fine wrinkles at the corners of eyes immediately after using the mask of example 1 and the deionized mask is reduced compared to the initial value (before use), which is probably because the effect of immediately moisturizing the eyes makes the dried fine wrinkles useless. From the long-term effect, the mask of the embodiment 1 of the invention has better effect of reducing the fine wrinkles at the corners of eyes.

In conclusion, the plant composition disclosed by the invention has better moisturizing and water-locking effects through reasonable collocation of dendrobium, tremella, ganoderma lucidum and saffron, and can effectively increase skin elasticity, reduce the number of fine wrinkles and prevent the growth of fine wrinkles at the corners of eyes. Skin elasticity and fine lines have a great relationship with skin aging, and as the age increases, collagen is lost and skin elasticity becomes worse. The plant composition has the effects of moisturizing, moisturizing and improving skin elasticity while being used as a matrix directly in cosmetics such as toning lotion, emulsion, cream, facial mask and the like, is not limited by the types and dosage forms of cosmetics and/or skin care products, and has wide market application prospect.

Claims

1. The plant composition is characterized by being prepared from the following components in parts by weight:

10-30g of tremella, 10-30g of dendrobium, 2-6g of ganoderma lucidum and 0.001-0.3g of saffron;

the plant composition is prepared by a preparation method comprising the following steps:

1) Soaking Tremella and herba Dendrobii, and panning;

4) Filtering and sterilizing;

the preparation pressure is 0.035-0.055MPa, the preparation temperature is 100-130 ℃ and the treatment time is 1.0-4.0h;

wherein, in terms of mass ratio, m/m, the feed liquid ratio of the plant raw material to water in the preparation method is 1:30-1:70.

2. The plant composition according to claim 1, wherein the plant composition is prepared from the following components in parts by weight: 10-30g of tremella, 10-30g of dendrobium, 2-6g of lucid ganoderma and 0.001-0.01g of saffron.

3. Use of a phytocomposition according to any one of claims 1 to 2 for the preparation of cosmetics, wherein said phytocomposition is directly used as a matrix for the preparation of cosmetics.

4. The use according to claim 3, wherein the cosmetic is a skin care product.

5. Use of a botanical composition as claimed in claim 3 in cosmetics as a substrate directly for the preparation of cosmetics which feel good on skin and/or moisturize and/or lock water and/or improve skin elasticity and/or reduce periocular fine wrinkles and/or prevent the growth of canthus fine wrinkles.