CN114081853A

CN114081853A - Plant composition and preparation method and application thereof

Info

Publication number: CN114081853A
Application number: CN202110325667.9A
Authority: CN
Inventors: 孟宏; 曲召辉
Original assignee: Taihe Kangmei Beijing Research Institute of Traditional Chinese Medicine Co Ltd
Current assignee: Taihe Kangmei Beijing Research Institute of Traditional Chinese Medicine Co Ltd
Priority date: 2021-03-26
Filing date: 2021-03-26
Publication date: 2022-02-25
Anticipated expiration: 2041-03-26
Also published as: CN114081853B

Abstract

The invention discloses a plant composition and a preparation method and application thereof, and belongs to the field of daily cosmetics. The plant composition comprises 10-30g of tremella, 10-30g of dendrobium, 2-6g of lucid ganoderma and 0.001-0.3g of saffron. The plant composition provided by the invention is prepared from the traditional Chinese medicine beauty aspect by utilizing the medicine and food homologous thought, supplementing the whole formula and scientifically matching, and has multiple effects and remarkable effects. The plant composition has the effects of comfortable skin feel, moisturizing, locking water, improving skin elasticity, reducing fine lines of canthus, preventing the fine lines of canthus from growing and the like, can be directly applied to skin care products as a natural plant raw material, can also be directly used as a substrate for cosmetics such as astringent, lotion, cream, facial mask and the like, and has wide market application prospect.

Description

Plant composition and preparation method and application thereof

Technical Field

The invention relates to the field of daily cosmetics, and in particular relates to a plant composition and a preparation method and application thereof.

Background

The living states of fast pace of life, high working pressure, insufficient sleep, lack of movement and the like cause various problems of the skin of modern people, such as dryness, water shortage, loss of elasticity, dark yellow skin color, large pores, sensitive peeling and the like. Moisture in the skin is an important factor for moistening the skin, keeping the skin healthy and preventing skin aging, and effective moisture-keeping nursing can delay skin aging, improve the tolerance of the skin to external stimulation and prevent skin diseases. At present, most of commercially available cosmetics can relieve the problem of dry skin to a certain extent, but cannot meet the skin care requirements of consumers for comprehensively improving the skin state from the aspect of skin health preservation. In recent years, along with the progress of times and the increase of people's advocating and natural returning demands, various cosmetics with traditional Chinese medicine efficacy produced by taking traditional Chinese medicine extracts as cosmetic additives are more and more sought after by consumers.

The patent (CN 201910649489.8) discloses a facial mask containing tremella and dendrobium extract and a preparation method thereof, wherein tremella and dendrobium extract with extremely high polysaccharide content are selected, other skin feel modifiers and other complex skin feel conditioners are not added, the components are simple, and natural silkiness and super-strong moisture retention and repair are achieved. But only the efficacy of moisturizing the facial mask is considered, and the skin care requirements of consumers on overall comprehensive improvement of the skin state are ignored.

Based on the above, it is necessary to develop a plant composition with comfortable skin feel, moisturizing, water locking, skin elasticity improving, fine lines of the canthus reducing and growth preventing effects by scientific compatibility.

Disclosure of Invention

Based on the above-mentioned drawbacks of the prior art, the present invention provides a plant composition having the effects of comfortable skin feel, moisturizing, locking water, improving skin elasticity, reducing fine lines of the canthus, and preventing the growth of fine lines of the canthus; the plant composition can be used as natural plant material directly applied in skin care products, and can be directly used as matrix in cosmetic water, lotion, cream, facial mask, etc.

The technical problem to be solved by the invention is realized by the following technical scheme:

according to the invention, the plant composition comprises the following components of tremella, dendrobium, ganoderma lucidum and saffron.

According to some specific embodiments of the present invention, the plant composition comprises the following components in parts by weight: 10-30g of tremella, 10-30g of dendrobium, 2-6g of lucid ganoderma and 0.001-0.3g of saffron crocus.

According to some specific embodiments of the present invention, the plant composition comprises the following components in parts by weight: 10-30g of tremella, 10-30g of dendrobium, 2-6g of lucid ganoderma and 0.001-0.01g of saffron crocus.

According to the present invention, the plant composition is prepared by a preparation method comprising:

1) soaking and washing Tremella and herba Dendrobii;

2) putting Ganoderma and stigma croci Sativi into filter bag, and mixing with cleaned Tremella and herba Dendrobii;

3) extracting the plant raw materials in the steps 1) and 2) with water according to a certain material-liquid ratio under the conditions of high temperature and high pressure;

4) filtering and sterilizing.

According to the invention, the preparation pressure in the preparation method is 0.035-0.055 MPa.

According to the invention, the preparation temperature in the preparation method is 100-130 ℃.

According to the invention, the treatment time in the preparation method is 1.0-4.0 h.

According to some embodiments of the invention, the preparation pressure in the preparation process is 0.04 MPa.

According to some embodiments of the invention, the preparation temperature in the preparation method is 110 ℃.

According to some embodiments of the invention, the treatment time in the preparation process is 2.0 h.

According to the invention, the feed-liquid ratio of the plant raw material to water in the preparation method is 1:30-1:70 (m/m).

According to some embodiments of the invention, the ratio of plant material to water in the preparation process is 1:45 (m/m).

According to the invention, the tremella is dry tremella, and large tremella pieces are properly torn into small pieces of 1-3 cm.

According to the invention, the filter bag is a non-woven fabric filter bag.

According to the invention, the extracting solution is filtered once by using 100-200-mesh filter cloth.

In a second aspect, the present invention provides a method for preparing a plant composition according to the first aspect of the present invention.

According to the invention, the preparation method comprises the following steps:

1) weighing 10-30g of tremella and 10-30g of dendrobium, soaking and elutriating;

2) weighing 2-6g of lucid ganoderma and 0.001-0.3g of saffron crocus, filling into a filter bag, and mixing with the washed tremella and dendrobium;

3) extracting the plant raw materials in the step 1) and the step 2) with water according to the material-liquid ratio of 1:30-1:70 (m/m) for 1.0-4.0h under the conditions of the preparation pressure of 0.035-0.055MPa and the preparation temperature of 100-;

4) filtering and sterilizing.

According to the invention, the filter bag is a non-woven fabric filter bag.

In a third aspect, the present invention provides the use of the plant composition of the first aspect or the plant composition prepared by the preparation method of the plant composition of the second aspect in cosmetics, wherein the plant composition can be directly used as a substrate in cosmetics such as astringent, lotion, cream, mask and the like and/or skin care products.

According to the invention, the botanical composition can be used directly as a base in a cosmetic product for good skin feel and/or moisturizing and/or locking water and/or improving skin elasticity and/or reducing fine lines around the eyes and/or preventing the growth of fine lines around the eyes.

According to the present invention, the cosmetic is not particularly limited in kind or formulation, and may be, for example, a mask, a cream, a face lotion, a essence, a milky lotion, or the like.

The invention has the beneficial effects that:

1. the plant composition disclosed by the invention starts from the aspect of traditional Chinese medicine cosmetology, utilizes the concept of homology of medicine and food, takes the effect of supplementing liquid and promoting the secretion of saliva or body fluid of dendrobium officinale, adopts the anti-allergy and anti-inflammation effects of lucid ganoderma, contains the water-retaining and screen-protecting function of tremella, and benefits of nourishing yin and moistening and nourishing saffron, utilizes scientific compatibility, fully complements each other, realizes optimal synergy, and has the effects of supplementing water, preserving moisture, locking water, improving skin elasticity, reducing fine lines around eyes, preventing the increase of fine lines at the corners of eyes and the like.

2. The plant composition of the invention adopts natural plants, has simple components and meets the requirements of modern people on natural plant raw materials.

3. The plant composition can be directly applied to skin care products as a natural plant raw material, has comfortable skin feel and excellent moisturizing effect, can be directly used as a substrate for cosmetics such as astringent, emulsion, cream, facial mask and the like, and has wide market application prospect.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the following will be briefly described.

FIG. 1 is a skin feel test chart of example 1, example 5 and comparative examples 2 to 9;

FIG. 2 is a graph showing the results of skin moisture content tests of example 1, example 5 and comparative examples 4 to 9;

FIG. 3 is a graph of the results of the canthus fine line test for examples 1, 5 and comparative examples 4-9;

FIG. 4 is a graph of the results of a skin moisture content test of the mask;

FIG. 5 is a graph of the skin elasticity R2 test results for a mask;

FIG. 6 is a graph of the results of the R5 test for skin elasticity of the mask;

FIG. 7 is a graph of the skin elasticity R7 test results for a mask;

fig. 8 is a graph showing the results of the canthus fine line test of the mask.

Detailed Description

The invention is further illustrated below with reference to specific examples, to which, however, the invention is not restricted.

The experimental methods described in the following examples are all conventional methods unless otherwise specified; the experimental materials and reagents are commercially available, unless otherwise specified.

The names of the main raw materials, suppliers/manufacturers and production places of the invention are shown in table 1:

TABLE 1 raw materials and sources

Name of raw materials	Supplier/producer	Producing area
			DendrobiumDendrobium officinale Kimura et Migo	Puer Darun Dendrobium officinale planting Co Ltd	Yunnan province
Tremella fuciformis berk	Kangwang food Co Ltd	Fujian tea
			Glossy ganoderma	Beijing Qiancao Chinese herbal pieces Limited	Beijing
Saffron crocus	Beijing Qiancao Chinese herbal pieces Limited	Beijing
			Hexanediol	De Zhi Xin (Shanghai) Co., Ltd	Germany
Pentanediol	De Zhi Xin (Shanghai) Co., Ltd	Germany

Example 1

Weighing 20g of tremella, 20g of dendrobium, 4g of lucid ganoderma and 0.01g of saffron crocus; firstly, soaking and washing tremella and dendrobium; putting Ganoderma and stigma croci Sativi into filter bag, mixing with washed Tremella and herba Dendrobii, adding 1980mL water at a material-liquid ratio of 1:45 (m/m), and extracting at 110 deg.C under 0.04MPa for 2.0 hr; filtering the extractive solution with 200 mesh filter cloth to obtain the plant composition.

Example 2

Weighing 10g of tremella, 10g of dendrobium, 2g of lucid ganoderma and 0.001 g of saffron crocus; firstly, soaking and washing tremella and dendrobium; putting Ganoderma and stigma croci Sativi into filter bag, mixing with washed Tremella and herba Dendrobii, adding 1540mL of water at a material-liquid ratio of 1:70 (m/m), and extracting at 100 deg.C under 0.035MPa for 2.0 h; filtering the extractive solution with 200 mesh filter cloth; obtaining the plant composition.

Example 3

Weighing 30g of tremella, 30g of dendrobium, 6g of lucid ganoderma and 0.3g of saffron crocus; firstly, soaking and washing tremella and dendrobium; putting Ganoderma and stigma croci Sativi into filter bag, mixing with washed Tremella and herba Dendrobii, adding 1980mL water at a material-liquid ratio of 1:30 (m/m), and extracting at 130 deg.C under 0.055MPa for 1.0 h; filtering the extractive solution with 200 mesh filter cloth; obtaining the plant composition.

Example 4

Weighing 20g of tremella, 20g of dendrobium, 4g of lucid ganoderma and 0.01g of saffron crocus; firstly, soaking and washing tremella and dendrobium; putting Ganoderma and stigma croci Sativi into filter bag, mixing with washed Tremella and herba Dendrobii, adding 1980mL water at a material-liquid ratio of 1:45 (m/m), and extracting at 130 deg.C under 0.055MPa for 2.0 hr; filtering the extractive solution with 200 mesh filter cloth to obtain the plant composition.

Example 5

Weighing 20g of tremella, 20g of dendrobium, 4g of lucid ganoderma and 0.01g of saffron crocus; firstly, soaking and washing tremella and dendrobium; putting Ganoderma and stigma croci Sativi into filter bag, mixing with washed Tremella and herba Dendrobii, adding 1980mL water at a material-liquid ratio of 1:45 (m/m), and extracting at 100 deg.C under 0.035MPa for 2.0 h; filtering the extractive solution with 200 mesh filter cloth to obtain the plant composition.

Comparative example 1

Weighing 20g of tremella, 20g of dendrobium, 4g of lucid ganoderma and 0.01g of saffron crocus; firstly, soaking and washing tremella and dendrobium; putting Ganoderma and stigma croci Sativi into filter bag, mixing with washed Tremella and herba Dendrobii, adding 1980mL water at a material-liquid ratio of 1:45 (m/m), extracting at 85 deg.C under normal pressure for 2.0 hr; filtering the extractive solution with 200 mesh filter cloth to obtain the plant composition.

Comparative example 2

Weighing 20g of dendrobium, 4g of lucid ganoderma and 0.01g of saffron crocus; firstly, soaking and elutriating the dendrobium; putting Ganoderma and stigma croci Sativi into filter bag, mixing with washed herba Dendrobii, adding 1080mL water at a material-liquid ratio of 1:45 (m/m), and extracting at 110 deg.C under 0.04MPa for 2.0 hr; filtering the extractive solution with 200 mesh filter cloth to obtain the plant composition.

Comparative example 3

Weighing 20g of tremella, 4g of lucid ganoderma and 0.01g of saffron crocus; firstly, soaking and washing tremella; putting Ganoderma and stigma croci Sativi into filter bag, mixing with washed Tremella, adding 1080mL water at a material-liquid ratio of 1:45 (m/m), and extracting at 110 deg.C under 0.04MPa for 2.0 hr; filtering the extractive solution with 200 mesh filter cloth to obtain the plant composition.

Comparative example 4

Weighing 20g of tremella, 20g of dendrobium and 4g of lucid ganoderma, and firstly soaking and washing the tremella and the dendrobium; putting Ganoderma into filter bag, mixing with washed Tremella and herba Dendrobii, adding 1980mL water at a material-liquid ratio of 1:45 (m/m), and extracting at 110 deg.C under 0.04MPa for 2.0 hr; filtering the extractive solution with 200 mesh filter cloth to obtain the plant composition.

Comparative example 5

Weighing 20g of tremella, 20g of dendrobium and 0.01g of saffron crocus, and firstly soaking and washing the tremella and the dendrobium; putting stigma croci Sativi into filter bag, mixing with washed Tremella and herba Dendrobii, adding 1800mL water at a material-liquid ratio of 1:45 (m/m), and extracting at 110 deg.C under 0.04MPa for 2.0 h; filtering the extractive solution with 200 mesh filter cloth to obtain the plant composition.

Comparative example 6

Weighing 20g of tremella and 20g of dendrobium; soaking and washing tremella and dendrobium; adding 1800mL of water, wherein the material-liquid ratio is 1:45 (m/m), and extracting for 2.0 h under the conditions that the preparation pressure is 0.04MPa and the preparation temperature is 110 ℃; filtering the extractive solution with 200 mesh filter cloth to obtain Tremella and herba Dendrobii composition.

Comparative example 7

Weighing 20g of tremella, 20g of dendrobium, 4g of lucid ganoderma and 0.01g of saffron crocus; firstly, soaking and washing tremella and dendrobium; putting Ganoderma and stigma croci Sativi into filter bag, mixing with washed Tremella and herba Dendrobii, adding 1980mL water at a material-liquid ratio of 1:45 (m/m), and extracting at 100 deg.C under 0.02 MPa for 2.0 hr; filtering the extractive solution with 200 mesh filter cloth to obtain the plant composition.

Comparative example 8

Weighing 20g of tremella, 20g of dendrobium, 4g of lucid ganoderma and 0.01g of saffron crocus; firstly, soaking and washing tremella and dendrobium; putting Ganoderma and stigma croci Sativi into filter bag, mixing with washed Tremella and herba Dendrobii, adding 1980mL water at a material-liquid ratio of 1:45 (m/m), and extracting at 135 deg.C under 0.06 MPa for 2.0 hr; filtering the extractive solution with 200 mesh filter cloth to obtain the plant composition.

Comparative example 9

Weighing 6g of lucid ganoderma, putting the lucid ganoderma into a filter bag, adding 270mL of water according to the material-liquid ratio of 1:45 (m/m), and extracting for 2.0 h under the conditions that the preparation pressure is 0.04MPa and the preparation temperature is 110 ℃; filtering the extractive solution with 200 mesh filter cloth to obtain Ganoderma extract.

Comparative example 10

Weighing 0.3g of saffron crocus, filling into a filter bag, mixing with the washed tremella and dendrobium, adding 13.5mL of water, wherein the material-liquid ratio is 1:45 (m/m), and extracting for 2.0 h under the conditions that the preparation pressure is 0.04MPa and the preparation temperature is 110 ℃; filtering the extractive solution with 200 mesh filter cloth to obtain stigma croci Sativi extract.

Comparative example 11

Weighing 5g of tremella, 20g of dendrobium, 20g of lucid ganoderma and 0.01g of saffron crocus; firstly, soaking and washing tremella and dendrobium; putting Ganoderma and stigma croci Sativi into filter bag, mixing with washed Tremella and herba Dendrobii, adding 2025mL water at a material-liquid ratio of 1:45 (m/m), extracting at 110 deg.C under 0.04MPa for 2.0 hr; filtering the extractive solution with 200 mesh filter cloth to obtain the plant composition.

Comparative example 12

Weighing 20g of tremella, 5g of dendrobium, 20g of lucid ganoderma and 0.01g of saffron crocus; firstly, soaking and washing tremella and dendrobium; putting Ganoderma and stigma croci Sativi into filter bag, mixing with washed Tremella and herba Dendrobii, adding 2025mL water at a material-liquid ratio of 1:45 (m/m), extracting at 110 deg.C under 0.04MPa for 2.0 hr; filtering the extractive solution with 200 mesh filter cloth to obtain the plant composition.

Basic index test of plant composition by different preparation processes

1.1 measurement of polysaccharide and Total sugar content

The method is carried out by adopting a phenol-sulfuric acid method (specifically referring to the content determination of polysaccharide in tremella [ J ]. Heilongjiang pharmaceutical science, 1999(06): 32-32.).

1.2 pH value: measured using a pH meter.

1.3 viscosity number: measured using a digital rotational viscometer.

1.4 solid content: measured by a solid content instrument.

1.5 skin feel and moisturizing effect: the evaluation method is crowd sensory evaluation, and after the smearing sample is tried out, the testers simply describe smearing feelings, including moistening feelings, astringency feelings, sticky feelings and moisturizing effects.

The preparation process has certain influence on the extraction efficiency, the efficacy expression and the like of the plant composition. The water extraction method is a common preparation method of plant extracts, and the method is simple and easy to implement. The plant composition prepared by water extraction method is used as a control to compare the influence of the extraction process on the basic properties and efficacy of the plant composition.

TABLE 2 index data of comparative example 1 and example 1

As shown in Table 2, the conventional water extraction method has low extraction efficiency, low polysaccharide content and total sugar content in the plant composition, and incomplete extraction of active substances. If the extraction efficiency of the water extraction method is to be improved, the raw materials need to be crushed in advance, so that the experimental manpower, material resources and time are greatly increased, meanwhile, the crushing also causes difficulty in filtration, and operation steps such as standing in advance, suction filtration and the like need to be added, so that the experimental time is doubled. The high-temperature high-pressure method is a method for extracting active ingredients under a closed condition with high pressure intensity, the extraction method can accelerate reaction during extraction, and meanwhile, water vapor can fully contact with all medicinal materials under the closed condition, so that the reaction efficiency is improved.

As can be seen from table 2, compared with comparative example 1, the high-temperature high-pressure process improves the polysaccharide content, total sugar content, viscosity and solid content of the plant composition, improves the extraction rate of active substances in the plant composition, avoids resource waste caused by insufficient utilization of raw materials, and greatly saves cost. Meanwhile, comparative examples 1 to 5, comparative example 7 and comparative example 8 have found that the high temperature and high pressure process conditions are important factors affecting the extraction rate. When the temperature and pressure of the high-temperature high-pressure treatment are lower than a certain range, the extraction rate of active substances of the plant extract is low, and the polysaccharide content, the total sugar content, the viscosity and the solid content are lower than those of the examples (example 5 and comparative example 7), so that the skin feeling can be slightly influenced; when the temperature and the pressure of the high-temperature high-pressure treatment exceed a certain range, the higher temperature and the higher pressure basically do not improve the extraction efficiency of the plants (example 4 and comparative example 8), and the skin feeling is not affected, but the too high pressure not only brings potential safety hazards, but also increases energy consumption and generates energy waste.

Tremella is rich in natural colloid, and can easily thicken solution, and when water extraction method with low temperature and pressure is adopted for low extraction, natural colloid in Tremella is difficult to extract, and when water extraction method is adopted for extraction, the texture of the extract is thin, and the extraction rate of active substances is low. Comparative example 1 (water extraction) the viscosity number of the extract was only 5.5 mPas, the texture was thin; example 1 (high temperature and high pressure method) the extract had a viscosity of 97.9 mPas and a thick texture similar to the essence. The extract liquid of example 1 (high temperature and high pressure method) has texture similar to essence liquid, and skin feeling is moist, and the astringent feeling and sticky feeling of the whole extract liquid of example 1 are reduced after herba Dendrobii is added. The solution of comparative example 1 (water extraction method) has poor moisturizing effect due to low extraction efficiency and incomplete extraction of effective components.

Basic index test of different raw material plant composition

In order to test the basic indexes of different raw material plant compositions, indexes such as pH value, viscosity (mPa.s), solid content (%), skin feeling, moisturizing effect and the like of each index of example 1, examples 4 to 5, comparative examples 2 to 6 and comparative examples 9 to 12 were further measured, and the test methods are as shown in the above 1.2 to 1.5.

TABLE 3 index data for different raw plant compositions

As shown in table 3, the plant composition components significantly affected the viscosity, skin feel and moisturizing effect of the plant composition. Compared with the comparative example 2, the example 1 has the characteristics of moistening feel, no astringent feel, light sticky feel, good moisturizing effect and the like, and has thick texture like essence; compared with the embodiment 1, the viscosity of the comparative example 2 is low, which shows that the tremella polysaccharide can increase the solution consistency and provide certain moist skin feeling, and the macromolecular polysaccharide can form a layer of protective film on the skin surface to enhance the moisturizing effect of the composition; comparative example 11 the moistening feeling was reduced but remained due to the reduced tremella usage and the change in solution consistency; compared with example 1, comparative example 3 has slightly astringent feeling because dendrobe has the efficacy of lubricating skin, the lack of dendrobe causes the skin feeling to be slightly poor, and the combination of dendrobe and tremella synergizes to cause the skin feeling to be moist and smooth; comparative example 12 reduces the amount of dendrobium, reduces the oily feeling of the solution, and produces some astringent feeling; comparative examples 4-6 had no significant difference in skin feel compared to example 1; comparative example 9 Ganoderma lucidum extract, similar in texture to water but slightly sticky; comparative example 10 is saffron extract, similar in texture to water. In conclusion, the plant composition has good skin feeling and moisturizing effect by reasonably matching the dendrobium, the tremella, the lucid ganoderma and the saffron crocus and the synergistic effect of the functional components.

Evaluation of efficacy of botanical compositions

In order to verify the efficacy of the product, and the effect of each ingredient on the product, example 1, example 5, and comparative examples 2-9 were screened from the comparative examples and examples in combination with the above data for relevant efficacy tests.

3.1 skin feel test

The samples were evaluated: example 1, example 5, comparative examples 2-9;

testing the population: a total of 45 persons (female, age 18-55 years, subject volunteer enrollment criteria);

the test method comprises the following steps: the 50 uL evaluation sample is respectively coated on the backs of the left hand and the right hand, the other hand is used for gently and circularly coating for 10 times, and then the thickness, the moistening feeling, the absorption speed, the astringency feeling and the sticky feeling of the sample are sequentially evaluated in a scoring mode (1-5 minutes), and the test result is shown in figure 1.

Consistency acceptance: indicating the acceptance of the subjects on the product appearance, 1 representing no acceptance and 5 representing acceptance;

moistening feeling: the moistening feeling of the skin after use, 1 represents very dry, and 5 represents very moist;

absorption speed: the absorption speed of the product during smearing is 1, which means that the product is not easy to absorb, and 5, which means that the product is easy to absorb;

astringency: the astringent feeling of the product during smearing, 1 represents very smooth, and 5 represents heavy astringent feeling;

sticky feeling: the sticky feeling of the skin after use, 1 represents no stickiness, and 5 represents very stickiness;

as shown in FIG. 1, example 1 had better moistening feeling, and the subjects scored more than 4 points. Compared with example 1, example 5 has little difference in skin feel from example 1; the extract of the comparative example 2 has poor moistening effect, the scores of subjects are below 3, the extract of the comparative example 2 has heavy sticky feeling and astringent feeling, and the satisfaction of most subjects is low, because the tremella is lacking, the tremella macromolecular polysaccharide is not contained in the extract, and the solution is thin and lacks moistening feeling; comparative example 3 the consistency of the extract is accepted by most subjects, but the sticky feeling is too heavy, the skin feel is sticky after the extract is applied, and the extract is not easy to absorb; comparative examples 4 to 6 are not very different in skin feel, but slightly different in color of the extract due to the difference between saffron and ganoderma lucidum; comparative example 7 has thinner consistency, lacks moist feeling, slightly sticky feeling; comparative example 8 skin feel was similar to that of clear water; comparative example 9 the moistening feeling was reduced because the amount of tremella was reduced and the consistency was slightly thin.

3.2 moisture content test

The samples were evaluated: example 1, example 5, comparative examples 2-9;

moisture content tests were performed for example 1, example 5, and comparative examples 2 to 9. Through the single human trial at the arm, utilize skin moisture content tester Corneometer CM825 to measure the moisturizing effect of evaluating sample in 4 hours after using, through the skin moisture content change before and after the contrast use, the evaluation sample improves the efficiency of skin moisture content.

30 (female, age 18-55, according to the volunteer selection standard of the subject) volunteers were selected to participate in the test, the evaluation samples were randomly distributed on the left and right forearm flexor sides of the volunteers, and the skin moisture content of the forearm flexor sides before, after 5min, 1h, 2h and 4h after the test, and the test results are shown in fig. 2.

As shown in FIG. 2, compared with the initial values (before use), the skin moisture content can be remarkably improved in the examples 1 and 5, and the comparative examples 4 to 6 and 95 min after use, and the comparative examples 7 to 8 have no remarkable effect of improving the skin moisture content; the moisture content of the skin gradually decreases with time after use. Compared with the comparative example, the skin can still have higher moisture content after 4 hours of use in example 1 and example 5, which shows that the skin has good moisture retention and water locking effects. And the moisturizing and moisturizing effects of the example 1 and the example 5 are superior to those of the plant compositions obtained in the comparative examples 2 to 6 and the single extracts obtained in the comparative examples 9 to 10, which shows that the plant composition of the present invention has better moisturizing and moisturizing effects and water locking effects than the composition prepared from any single extract and any combination of two or three of the extracts. Experimental data show that the plant composition has the advantages of synergistic effect through reasonable proportioning of the dendrobium, the tremella, the saffron crocus and the lucid ganoderma, and has better effects of moisturizing and locking water.

3.3 reduction of canthus Slim testing

The samples were evaluated: example 1, example 5, comparative examples 2-9;

testing the population: a total of 45 (women, age 18-55 years, subject volunteer enrollment criteria);

the test method comprises the following steps: appropriate amount of equal amount of evaluation samples are respectively smeared on the left canthus and the right canthus every morning and evening, and the fine lines of the canthus 30min, 1 week and 2 weeks before and after the use of the volunteers are tested by an EvasKIN instrument, and the test results are shown in figure 3.

As can be seen from fig. 3, after 30min of use, examples 1 and 5, comparative examples 5 and 9 all had a certain effect of reducing the fine lines in the canthus, and the difference between the remaining samples was small, and there was almost no effect, as compared with the initial value (before use). However, from the long-term effect, compared with comparative example 5 and comparative example 9, examples 1 and 5 have a significant effect of reducing the fine lines of the canthus after 2 weeks of use, i.e., examples 1 and 5 have the efficacy of reducing the fine lines of the canthus for a long time and preventing the increase of the fine lines of the canthus. Comparative examples 4, 5 and 8 did not have a significant effect of reducing the canthus striae, but the striae were substantially kept from increasing, whereas comparative examples 6 and 7 did not have an effect of reducing the striae. The experimental data further show that the botanical compositions of the invention have better efficacy in reducing fine lines in the canthus than compositions prepared from any single extract and any combination of two or three of the materials. The plant composition has the effects of reducing fine lines of canthus for a long time and preventing the increase of the fine lines of the canthus by reasonably proportioning the dendrobium, the tremella, the saffron and the lucid ganoderma and realizing synergistic interaction.

Preparation method of facial mask

The experimental results show that the plant composition has good skin feel and has the effects of moisturizing, reducing fine wrinkles at the canthus and the like. To further study the application of the plant composition of the present invention in the cosmetic field, the facial mask prepared in example 1 was used for the trial of the population to investigate the efficacy of the plant composition in the cosmetic application. The present invention is not particularly limited in the kind and formulation of the cosmetic, and the kind of the cosmetic known in the art may be used, for example, facial mask, cream, face lotion, essence, lotion, etc.

The specific raw materials of the mask and the preparation method are as follows:

TABLE 4 facial mask formula raw materials and mixture ratio

The preparation method of the facial mask comprises the following steps:

adding the raw materials into a beaker according to the formula raw materials and the proportion of the facial mask shown in the table 4, heating at 95 ℃ for 30min, cooling, soaking the facial mask cloth, and finally packaging into a facial mask bag.

Evaluation of mask use Effect

The test method comprises the following steps:

selecting 50 (female, age 18-55, according to subject volunteer selection standard) volunteers, dividing into 2 groups of 25 persons, wherein 1 group uses the facial mask of example 1 on the left face and uses deionized water facial mask on the right face; the deionized water mask was used for the left face and the mask of example 1 was used for the right face of group 2.

After face cleaning, the face is pasted on the back half of the face for 15min at night, the face is continuously used for 2 weeks, the face is used once every other day, and skin index tests including skin moisture content, skin elasticity R2/R5/R7 value and fine lines of canthus are respectively carried out on the volunteers for initial value, 5min, 30min, one week and two weeks. Wherein the facial mask has instant effect 5min and 30min after application, and can be directly tested without washing face after application; the long-term effect is shown in 1 week and 2 weeks, and the test is carried out after the face is washed with water and then statically sitting for 30 min. The skin elasticity R2/R5/R7 value R2 is the total elasticity, and the closer the value is to 1, the higher the skin elasticity is; r5 is net elasticity, the closer the value is to 1, the higher the skin elasticity; r7 is the elastic recovery capability, the closer the value is to 1, the higher the skin elasticity.

The skin moisture content was measured using a skin moisture content tester, Corneometer CM825, the skin elasticity was measured using a skin elasticity tester, Cutometer MPA580, and the number of fine lines in the canthus was measured using an evaski instrument.

As shown in fig. 4, compared with the initial value (before use), the moisture content of the skin can be instantly increased after 5min by using the deionized water mask and the mask of the embodiment 1 of the invention, and the moisture content of the skin is higher after using the mask of the embodiment 1. After the facial mask is used for 30min, the moisture content of the skin using the deionized water facial mask is rapidly reduced, the moisture of the epidermis layer of the skin is taken away while the moisture is evaporated, and the facial skin becomes drier like a skin care product which is not smeared after face washing. From the long-term effect, after two weeks of use, the facial mask of example 1 gradually increased the skin moisture content, while the deionized water facial mask did not have an increasing effect on the skin moisture content, indicating that the plant composition of the present invention has good moisturizing and water locking effects.

As shown in figures 5-7, compared with the initial value (before use), the skin elasticity of the deionized water mask and the mask of the invention in the embodiment 1 is better improved in the values of R2/R5/R7 immediately and for a long time after the mask of the invention in the embodiment 1 is used, and the plant composition has the effect of improving the skin elasticity.

The number of fine lines of the canthus using the mask of example 1 and the deionized mask of the present invention is shown in fig. 8, and the number of immediate fine lines of the canthus after using the mask of example 1 and the deionized mask is reduced compared to the initial value (before use), which is probably because the immediate moisturizing effect makes the dry fine lines disappear. From the long-term effect, the facial mask of the embodiment 1 of the invention has better effect of reducing fine wrinkles of the canthus.

In conclusion, the plant composition has better moisturizing and water locking effects through reasonable matching of the dendrobium, the tremella, the lucid ganoderma and the saffron, effectively increases the skin elasticity, reduces the number of fine lines and prevents the increase of fine lines of canthus. Skin elasticity and fine lines have a great relationship with skin aging, and as the skin ages, collagen is lost, and the skin elasticity becomes worse and worse. The plant composition has the effects of moisturizing, moisturizing and locking water, simultaneously has the effect of improving the skin elasticity, can be directly used as a substrate for cosmetics such as astringent, emulsion, cream, mask and the like, is not limited by cosmetics and/or skin care varieties and dosage forms, and has wide market application prospect.

Claims

1. The plant composition is characterized by comprising the following components in parts by weight: 10-30g of tremella, 10-30g of dendrobium, 2-6g of lucid ganoderma and 0.001-0.3g of saffron crocus.

2. The plant composition according to claim 1, wherein the plant composition comprises the following components in parts by weight: 10-30g of tremella, 10-30g of dendrobium, 2-6g of lucid ganoderma and 0.001-0.01g of saffron crocus.

3. The plant composition according to claim 1 or 2, wherein the plant composition is prepared by a preparation method comprising the steps of:

1) soaking Tremella and herba Dendrobii, and elutriating;

4) filtering and sterilizing.

4. The plant composition as claimed in claim 3, wherein the preparation pressure is 0.035-0.055MPa, the preparation temperature is 100-130 ℃, and the treatment time is 1.0-4.0 h.

5. The plant composition according to claim 3, wherein the ratio of plant material to water in the preparation method is 1:30 to 1:70 (m/m).

6. A method of preparing a botanical composition, said method comprising the steps of:

weighing 10-30g of tremella and 10-30g of dendrobium, soaking and elutriating;

weighing 2-6g of lucid ganoderma and 0.001-0.3g of saffron crocus, filling into a filter bag, and mixing with the washed tremella and dendrobium;

4) filtering and sterilizing.

7. Use of a plant composition according to any one of claims 1 to 5 or prepared by a process for the preparation of a plant composition according to claim 6 in cosmetics, characterized in that the plant composition can be used directly as a base in cosmetics and/or skin care products.

8. Use of a plant composition according to claim 7 in cosmetics, characterized in that the plant composition can be used directly as a base in cosmetics for good skin feel and/or moisturizing and/or locking water and/or improving skin elasticity and/or reducing fine lines around the eyes and/or preventing the growth of fine lines at the corners of the eyes.