CN109394640A - A kind of ferment skin care compositions and its application - Google Patents
A kind of ferment skin care compositions and its application Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/63—Steroids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/676—Ascorbic acid, i.e. vitamin C
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Abstract
The invention discloses a kind of ferment skin care compositions and its applications.The skin care compositions comprises the following components in parts by weight: 4~9 parts of plant enzyme, 2~5 parts of Crithmum maritimum stem cell extract, 0.5~2.5 part of glycyrrhetinic acid and 1~3 part of ascorbic acid glucoside.Each ingredient of the skin care compositions is mutually coordinated, has stronger scavenging effect to free radical, can inhibit the activity of tyrosine kinase, the generation of melanin, the anti-ageing significant effect of whitening are reduced, while being easy to by percutaneous absorption, moisturizing water lock effect is good, can improve skin quality, promotes elasticity of skin.
Description
Technical field
The invention belongs to cosmetic technical fields, and in particular to a kind of ferment skin care compositions and its application.
Background technique
Ferment is that one kind contains enzyme and its microbes producing cellulase and the associated adjustment factor and interaction factor, is such as activated
The substance of agent and inhibitor, collaboration and the feedback regulation factor etc..There are a variety of ferment in human skin, to help epidermal cell
Regeneration and the secretion of sebaceous glands, sweat gland.As the age increases, internal ferment is opposite to be reduced, skin aging.Utilize modern biotechnology
Prepared by technology is added in beauty product the ferment of skin beneficiating, to reinforce the function of skin, improves skin.Different ferment
Element has different efficacies: some ferment facilitate the growth of deep skin cell, the aging of delaying skin；It can be with there are also ferment
Injury of the ultraviolet light to skin is resisted, the generation of melanin is inhibited；There are also some skin cell exfoliations that can make aging, even
Promote the formation of collagen and elastin in skin.With the increase at age, ferment opposite can be reduced, and at this moment just will appear skin
Problem.Therefore, ferment becomes a kind of emerging cosmetic industry additive.
However, ferment is applied in skin care item, there are still some problems: ferment molecule is unstable, and life cycle is short, one
As ferment in the intracorporal life cycle of body by a few hours to several weeks etc., well below the shelf life of skin care item；Ferment is usual
Stable not as good as other cosmetic materials, activity is easy to be influenced by soda acid, high temperature and surfactant, therefore in ferment
It needs to pay special attention in cosmetic preparation process；Ferment is in cosmetics such as improper use, it may appear that pruitus, decortication and allergy etc.
Adverse reaction.Therefore, ferment is incorporated into skin care item, is not easy thing, China is to the understanding of ferment cosmetics also in a step
Section, the product for actually entering market is relatively fewer, and most of cosmetics containing ferment are foreign brand name, such as European Biotherm,
Japanese Shiseido, Kosé, the face shop of South Korea etc..
The Chinese patent application of 103690469 A of Publication No. CN disclose a kind of shin moisturizer containing gingko ferment and
Preparation method and application, the shin moisturizer include gingko ferment and skin matrix, and the gingko ferment is by ginkgo kernel, apple
16 kinds of fruits and vegetables such as fruit, jujube and herbal raw material are fermented obtained, which has the effect of dewrinkling spot-eliminating whitening, can be used for
Prepare facial mask.But raw material is relative complex, many kinds of, higher cost.
Summary of the invention
The object of the present invention is to provide a kind of ferment skin care compositions and its applications.The skin care compositions is respectively at split-phase
Mutually coordinate, there is stronger scavenging effect to free radical, can inhibit the activity of tyrosine kinase, reduce the generation of melanin, beauty
White anti-ageing significant effect, while being easy to by percutaneous absorption, moisturizing water lock effect is good, can improve skin quality, promotes elasticity of skin.
The present invention is achieved by the following technical programs:
A kind of ferment skin care compositions, comprises the following components in parts by weight: 4~9 parts of plant enzyme, Crithmum maritimum stem cell mention
Take 2~5 parts of object, 0.5~2.5 part of glycyrrhetinic acid and 1~3 part of ascorbic acid glucoside.
It is preferred that the ferment skin care compositions, comprises the following components in parts by weight: 6~8 parts of plant enzyme, Crithmum maritimum
3~5 parts of stem cell extract, 1~2 part of glycyrrhetinic acid and 2~3 parts of ascorbic acid glucoside.
It is preferred that the ferment skin care compositions, comprises the following components in parts by weight: 7 parts of plant enzyme, Crithmum maritimum are dry thin
5 parts of born of the same parents' extract, 1.5 parts of glycyrrhetinic acid and 2.5 parts of ascorbic acid glucoside.
The plant enzyme includes that following parts by weight prepare raw material: 10~20 parts of wheat, 10~20 parts of tremella and sweet
5~15 parts of grass.
It is preferred that the plant enzyme includes that following parts by weight prepare raw material: 15 parts of wheat, 15 parts of tremella and Radix Glycyrrhizae
10 parts.
Plant enzyme of the invention is prepared by the following method to obtain, method includes the following steps:
(1) wheat, tremella, Radix Glycyrrhizae of formula ratio are weighed, is mixed, is crushed, the water of 10~12 times of its dry weight is added, impregnates 1
~2h, decocts 1~2h, and filtering obtains filter residue and Aqueous extracts；
(2) Aqueous extracts are concentrated into the one third of original volume at 55~60 DEG C, obtain concentrate；
(3) concentrate that filter residue that step (1) obtains, step (2) obtain is added into fermentor, it is warming up to 100~
120 DEG C, sterilize 30~45min, is cooled to 25~30 DEG C, and Bacillus acidi lactici is added, then Cattell is added in 48~72h of anaerobic fermentation
Saccharomycete is passed through clean compressed air toward fermentor, carries out under conditions of 25~30 DEG C, 400~800rpm/min aerobic
48~72h of fermentation by filtering fermentation liquor, obtains supernatant after fermentation；
(4) supernatant that step (3) obtains is filtered using sterilizing filter, collects bacteria-free filtrate, concentrates the filtrate to
The one third of original volume, obtains plant enzyme.
In above-mentioned plant enzyme preparation method, in step (3) inoculum concentration of Bacillus acidi lactici be fermentation material total weight 2~
4%, the inoculum concentration of saccharomyces carlsbergensis bacterium is the 1~3% of fermentation material total weight.
Ferment skin care compositions provided by the invention can be used for preparing skin care item；The skin care item are including but not limited to refreshing
Skin water, lotion, face cream, facial mask, eye cream or Essence.
Ferment skin care compositions of the invention includes plant enzyme, Crithmum maritimum stem cell extract, glycyrrhetinic acid and resists
Bad hematic acid glucoside, each component compounding rationally, have significant synergistic function, can achieve whitening, it is anti-ageing, go wrinkle, protect
A variety of skin effects such as water moisturizing.Wherein, plant enzyme is formed by 3 kinds of wheat, tremella, Radix Glycyrrhizae raw materials through microbial fermentation, hair
Ferment process safety is controllable, and tunning is pollution-free, non-stimulated, highly-safe, contain microelement abundant, amino acid, oligopeptides,
Vitamin, minerals, enzyme isoreactivity ingredient can help activating skin cells, promote metabolism, effectively reparation aged skin,
Oxidation resistance is improved, skin water content is increased.Crithmum maritimum stem cell extract is the main original of skin progenitor cell metabolism
Material and source can quickly activate suspend mode stem cell, and stem cell self-regeneration is promoted to update, the first viability of reduction epidermal stem cells,
Keep Skin Cell newborn in a steady stream, thus reach promotion, firm skin, desalinate wrinkle anti-aging effects, especially with plant enzyme
When being used in compounding, reach ultimate attainment anti-ageing effect.Glycyrrhetinic acid has good antibacterial and anti-inflammation functions, and can effectively releive skin,
Skin care compositions is reduced to the irritation of skin, promotes the comfort level of skin.Ascorbic acid glucoside is VB5Derivative, can
Inhibit the activity of tyrosinase, reduces melanin production.
Ingredient in inventive formulation is unless otherwise instructed commercial product.Wherein, Crithmum maritimum stem cell extract
For the CELTOSOME of match Bicc Public Ltd. Co, France productionTMCrithmum maritimum.Glycyrrhetinic acid CAS 471-53-4,
Purchased from Xi'an Rayleigh Biotechnology Co., Ltd.Ascorbic acid glucoside CAS 129499-78-1, purchased from the fragrant biology of Guangzhou card
Science and Technology Ltd..
Compared with prior art, the invention has the following advantages:
Each ingredient of ferment skin care compositions provided by the invention is mutually coordinated, promotes skin metabolism, and it is thin to assign skin living
Born of the same parents, it is obscure to improve skin, and it is glossy to improve skin, so that skin water is moistened smooth, high resilience, has efficient skin whitening, moisturizing, anti-ageing
Tender skin effect.Meanwhile property is mild, without toxic side effect, good penetrability is easy to absorb, and there is certain conditioning to make skin
With skin quality can be improved.
Specific embodiment
The following examples are further illustrations of the invention, rather than limiting the invention.
The preparation of 1 plant enzyme of embodiment
Plant enzyme includes that parts by weight shown in following table prepare raw material:
| Raw material | A group | B group | C group | D group |
| Wheat | 15 | 10 | 20 | 15 |
| Tremella | 15 | 20 | 10 | 15 |
| Radix Glycyrrhizae | 10 | 15 | 10 | 5 |
Preparation step:
(1) wheat, tremella, Radix Glycyrrhizae of formula ratio are weighed, is mixed, is crushed, the water of 10 times of its dry weight is added, impregnates 2h, is decocted
2h is boiled, filters, obtains filter residue and Aqueous extracts；
(2) Aqueous extracts are concentrated into the one third of original volume at 55 DEG C, obtain concentrate；
(3) concentrate that filter residue that step (1) obtains, step (2) obtain is added into fermentor, is warming up to 100 DEG C,
Sterilize 45min, is cooled to 30 DEG C, and Bacillus acidi lactici is added, and the inoculum concentration of Bacillus acidi lactici is the 3% of fermentation material total weight, anaerobism hair
Then saccharomyces carlsbergensis bacterium is added in ferment 72h, the inoculum concentration of saccharomyces carlsbergensis bacterium is the 2% of fermentation material total weight, is passed through toward fermentor clean
Net compressed air carries out aerobic fermentation 72h, after fermentation, by fermentation liquid mistake under conditions of 25 DEG C, 600rpm/min
Filter, obtains supernatant；
(4) supernatant that step (3) obtains is filtered using sterilizing filter, collects bacteria-free filtrate, concentrates the filtrate to
The one third of original volume, obtains plant enzyme.
The preparation of 2 ferment skin care compositions of embodiment
The preparation of the present embodiment ferment skin care compositions includes the component of parts by weight shown in following table:
Preparation method: by parts by weight shown in upper table, formula each component raw material is weighed respectively, is uniformly mixed to get ferment
Skin care compositions.
The preparation of 1 ferment skin care compositions of comparative example
The preparation of this comparative example ferment skin care compositions includes the component of parts by weight shown in following table:
Apple stem cells culture extract in upper table is the production of the biochemical corp Switzerland meter Bai Le
PhytocelltecTMMalus Domestica, the dried apple slices cell extract are using rare Switzerland apple stem cell as base
Plinth and the liposomes product developed, main component be Switzerland's apple berry extract, xanthan gum, glycerol, lecithin, Phenoxyethanol with
And water.Lactobacillus fermentation product is purchased from U.S. Ai Ti scientific & technical corporation (Active Concepts), article No. 20440.
Preparation method: by parts by weight shown in upper table, formula each component raw material is weighed respectively, is uniformly mixed to get ferment
Skin care compositions.
The preparation of 3 Essence of skin care compositions containing ferment of embodiment
(1) it is formulated:
Surplus is deionized water.
(2) preparation method: embodiment 2 is formulated 1 ferment skin care compositions, EDETATE SODIUM, glycine betaine, glycerol and is added to
Ionized water is heated to 37 DEG C and is uniformly mixed, and 100 revs/min of stirring rate；After mixing, 28 DEG C of addition propylene are cooled to
Acyl dimethyltaurine ammonium/VP copolymer, Sensiva SC50 and Phenoxyethanol is uniformly mixed, and is dispensed to get Essence.
The Essence preparation of the ferment skin care compositions of 2-5 and the formula of comparative example 1 6-9 is formulated with reference to above-mentioned containing embodiment 2
Shown, the additional amount of corresponding ferment skin care compositions is 18wt% in each test example.
The preparation of 4 toner of skin care compositions containing ferment of embodiment
(1) it is formulated:
Surplus is deionized water.
(2) preparation method: embodiment 2 is formulated 1 ferment skin care compositions, glycerol, butanediol, glycine betaine, xanthan gum and is added
Enter to be heated to 37 DEG C to deionized water and be uniformly mixed, 100 revs/min of stirring rate；After mixing, 28 DEG C are cooled to add
Enter methyl hydroxybenzoate and Phenoxyethanol, be uniformly mixed, dispenses to get toner.
The toner preparation of the ferment skin care compositions of 2-5 and the formula of comparative example 1 6-9 is formulated with reference to above-mentioned containing embodiment 2
Shown, the additional amount of corresponding ferment skin care compositions is 8wt% in each test example.
The preparation of 5 face cream of skin care compositions containing ferment of embodiment
(1) it is formulated:
Surplus is deionized water.
(2) preparation method:
1. water, propylene glycol, glycerol, triethanolamine, xanthan gum are added in emulsion pot, it is heated to 80 DEG C and stirs to complete
Fully dissolved obtains material A；
2. by isohexadecane, stearic acid, cetostearyl alcohol, caprylic/capric triglyceride, Polyoxyethylene Sorbitan Monooleate, poly- diformazan
Radical siloxane is added in oil cauldron, is heated to 80 DEG C of stirrings to being completely dissolved, obtains material B；
3. the material B is pumped into the material A, stir evenly, open homogeneous, constant temperature stirring is cooling；
4. being cooled to 40 DEG C, embodiment 2 is added and is formulated 1 skin care compositions containing ferment, methyl hydroxybenzoate and Phenoxyethanol, sufficiently
It stirs evenly, it is cooling, obtain anti-radiation sun screen.
The face cream preparation for being formulated the ferment skin care compositions of 2-5 and the formula of comparative example 1 6-9 containing embodiment 2 refers to above-mentioned institute
Show, the additional amount of corresponding ferment skin care compositions is 15wt% in each test example.
Test example one, irritation test
(1) face cream of the ferment skin care compositions of 1-5 and the formula of comparative example 1 6-9 tested material: is formulated containing embodiment 2.
(2) by the requirement of " cosmetics safety technical specification " 2015 editions, select 90 ages 20~50 years old aspiration by
Study subject of the examination person as test.90 subjects are equally divided into 9 groups, tries out be formulated 1-5 and comparison containing embodiment 2 respectively
Example 1 is formulated the face cream of the ferment skin care compositions of 6-9.
(3) experimental method: accurate weighing 0.02g face cream is put into spot tester (area 30mm2, depth 1mm) small interior, use is low
Sensitization adhesive tape will be pasted on the forearm song side of subject added with the spot tester of face cream, is gently pressed with palm and is allowed to equably be pasted on skin
On skin, after persistently sticking for 24 hours, 30min after taking away spot tester, for 24 hours with the standard sight dermoreaction of 48h following table and is recorded
Observe result.
As the result is shown: the dermoreaction degree of all subjects is-, grading system 0.Illustrate to be formulated containing embodiment 2
1-5 and comparative example 1 are formulated the face cream of the ferment skin care compositions of 6-9 to no skin irritation, further illustrate ferment of the invention
It is mild, non-stimulated that plain skin care compositions and the present invention prepare material used in face cream.
Test example two, antioxidant effect test
According to free radical injury theory, the generation of free radical is to lead to skin aging main cause.Therefore, if there is energy
The ability for removing free radical is to evaluate one of the important indicator of anti-aging skin care product.
DPPH (DPPH.free radical) is a kind of stable free radical centered on nitrogen, if tested material can be clear
Except it, then it represents that tested material has the function of reducing the free radicals such as hydroxy radical, alkane free radical or peroxy radical.DPPH exists
520nm has strong absorption, and ethanol water is dark purple, and after tested material is added, in 520nm, using spectrophotometric determination, it is clear
The case where causing absorbance to reduce except DPPH can obtain the ability that tested material removes DPPH according to absorbance change.Other are clear
Except the experimental principle of superoxide anion, hydroxy radical is same as above.
Detecting instrument: DPPH (Tokyo is melted into Co., Ltd., analyzes pure)；UV-1600 ultraviolet specrophotometer
(METTLETOLEDO)。
Detection method: embodiment 2 is formulated the ferment skin care compositions of 1-5 respectively with dimethyl sulfoxide and comparative example 1 is matched
It is the solution that mass volume ratio is 1% that the ferment skin care compositions of square 6-9, which is configured to concentration, carries out experiment below.
(this is molten for the DPPH solution that the measurement of radicals scavenging effect: (1) taking sample solution 2mL and concentration is 100 μm of ol/L
The solvent of liquid is for water) 2mL is first added in same tool plug test tube, shakes up, 30 minutes are stood at room temperature, using solvent as blank, in
Sample absorbance is measured under 517nm wavelength.
Inhibiting rate: K%=[1- (Ai-Aj)/AC] × 100%, Ai:2mLDPPH solution+2mL sample solution absorbance,
The absorbance of Aj:2mL sample solution+2mL solvent (i.e. dimethyl sulfoxide), AC:2mL DPPH solution+2mL solvent (i.e. dimethyl
Sulfoxide) absorbance.The measurement result of specific radicals scavenging effect is shown in Table 1.
The measurement result of the ferment skin care compositions radicals scavenging effect of each test group of table 1
The results show that the ferment skin care compositions that embodiment 2 is formulated 1-5 all has preferable free radical scavenging ability,
The effect of middle formula 1 is best, is up to 92% to the inhibiting rate of DPPH, hence it is evident that better than the ferment skin care group that comparative example 1 is formulated 6-9
Close object.Show ferment skin care compositions each group distribution ratio science of the invention, it is mutually coordinated to play optimal antioxidant effect.
Test example three, moisturizing, moistening effect test
The Essence for the ferment skin care compositions that 1-5 and the formula of comparative example 1 6-9 are formulated containing embodiment 2 is mended respectively
Water, moistening effect test.
(1) test method: 90 healthy volunteers are chosen, the age is between 25~55 years old.By all subjects
9 test groups are randomly divided into, respectively test 1-5 group and comparison 1-4 group, wherein test 1-5 group is matched using containing embodiment 2 respectively
The Essence of the ferment skin care compositions of square 1-5, comparison 1-4 group use the ferment skincare composition that 6-9 is formulated containing comparative example 1 respectively
The Essence of object.It takes appropriate Essence to be uniformly applied to face after subject's morning and evening face cleaning of all test groups, massages 2~3min
To fully absorbing.Trial employment period prevents solar exposure, and is forbidden to use other skin care item.Continuous use 1 month after, to it is all by
Examination person carries out moisturizing, the detection of moistening effect and record.
(2) effect assessment standard: 1. skin moisture content: the skin moisture tester of CK company, Germany is utilized
(Corneometer CM825) measures the moisture content of subject's face skin, and moisture content climbing is bigger, shows that moisturizing is imitated
Fruit is better.2. skin elasticity: detecting subject's face flesh using the spring probe of Callegari soft plus skinanalysis apparatus
The initial elasticity m of skin0Value, duplicate measurements 5 times, is averaged, in order to preferably evaluate the data obtained, by the m detected every time points
Not divided by the m of unused sample0Average value obtains the elasticity grading (the elasticity grading before tested is 1 grade) at the position, works as bullet
Property grading value it is bigger, illustrate sample increase skin elasticity effect be more obvious.
It the results are shown in Table 2 and 3.
The measurement result of each test group moisture content of skin of table 2 variation
The measurement result of each test group skin elasticity of table 3 variation
As shown in Table 2, Essence moisturizing, the moistening effect that the ferment skin care compositions of 1-5 is formulated containing embodiment 2 are good, make
Skin moisture content can not only be significantly improved after, can also lock moisture, so that skin is kept long-acting water profit, hence it is evident that better than containing comparison
Example 1 is formulated the Essence of the ferment skin care compositions of 6-9.
As shown in Table 3, the Essence that the ferment skin care compositions of 1-5 is formulated containing embodiment 2 can effectively increase the bullet of skin
Property, hence it is evident that better than the Essence for the ferment skin care compositions for being formulated 6-9 containing comparative example 1, wherein with the ferment containing the formula of embodiment 21
The elasticity grading highest of the Essence of plain skin care compositions shows ferment skin care compositions each group distribution ratio science of the invention,
It is mutually coordinated to play optimal anti-ageing effect.
Test example four, whitening effect test
Beauty is carried out to the Essence for the ferment skin care compositions for being formulated 1-5 and the formula of comparative example 1 6-9 containing embodiment 2 respectively
White effect test.
(1) test method: 90 healthy volunteers are chosen, the age is between 25~55 years old.By all subjects
9 test groups are randomly divided into, respectively test 1-5 group and comparison 1-4 group, wherein test 1-5 group is matched using containing embodiment 2 respectively
The Essence of the ferment skin care compositions of square 1-5, comparison 1-4 group use the ferment skincare composition that 6-9 is formulated containing comparative example 1 respectively
The Essence of object.It takes appropriate Essence to be uniformly applied to face after subject's morning and evening face cleaning of all test groups, massages 2~3min
To fully absorbing.Trial employment period prevents solar exposure, and is forbidden to use other skin care item.Continuous use 1 month after, to it is all by
Examination person carries out detection and the record of whitening effect.
(2) skin care item of the present invention effect assessment standard: are evaluated using the change rate of dermal melanin content and brightness
Whitening effect, the specific method is as follows: 1. dermal melanin content: using Japanese Minolta CD-2600 spectrophotometric analysis
The melanin content situation of change of subject's face skin.It uses Lab color system, collects the reflection vertical with skin surface
Light, the b reflection in measurement parameter from Huang to blue color variation range (value variation range :+60 yellow~-60 blues),
It is positively correlated with the black pigment of skin content, this is related with melanin reflection yellow light.Before on-test, first to each subject's flesh
The melanin content of skin is detected, and is recorded；After test 1 month, the melanin of each subject's skin is carried out again
Detection, and record.The melanin rate of descent of skin: melanin rate of descent=(numerical value-test before testing is calculated using following formula
Numerical value afterwards)/test preceding numerical value × 100%.Melanin content rate of descent is bigger, shows that whitening effect is better.2. skin brightness:
Using the brightness of skin measurement system (VISIA) measurement skin, the skin the bright, is worth higher, and the skin the dim, be worth more
It is low.Before on-test, first the brightness of each subject's skin is detected, and records；After test 1 month, again
The brightness of each subject's skin is detected, and is recorded.The variation of the brightness of skin is calculated using following formula
Rate: numerical value × 100% before brightness change rate=(numerical value before numerical value-test after test)/test.Brightness climbing is bigger,
Show that whitening effect is better.
It the results are shown in Table 4.
Each test group whitening effect test result of table 4
| Group | Average black element rate of descent (%) | Average luminance climbing (%) |
| Test 1 group | 36.2 | 18.3 |
| Test 2 groups | 34.0 | 17.2 |
| Test 3 groups | 33.4 | 16.8 |
| Test 4 groups | 35.2 | 17.9 |
| Test 5 groups | 32.6 | 16.5 |
| Compare 1 group | 19.5 | 10.0 |
| Compare 2 groups | 21.7 | 12.2 |
| Compare 3 groups | 30.2 | 16.0 |
| Compare 4 groups | 29.0 | 15.9 |
The results show that dermal melanin can be significantly reduced containing the Essence that embodiment 2 is formulated the ferment skin care compositions of 1-5
Level promotes skin brightness, hence it is evident that better than the Essence for the ferment skin care compositions for being formulated 6-9 containing comparative example 1.Show this hair
Bright ferment skin care compositions each group distribution ratio science, it is mutually coordinated to play optimal whitening effect.
The above is only the preferred embodiment of the present invention, it is noted that above-mentioned preferred embodiment is not construed as pair
Limitation of the invention, protection scope of the present invention should be defined by the scope defined by the claims..For the art
For those of ordinary skill, without departing from the spirit and scope of the present invention, several improvements and modifications can also be made, these change
It also should be regarded as protection scope of the present invention into retouching.
Claims (10)
1. a kind of ferment skin care compositions, which is characterized in that comprise the following components in parts by weight: 4~9 parts of plant enzyme, extra large fennel
2~5 parts of smoked bean curd cell extract, 0.5~2.5 part of glycyrrhetinic acid and 1~3 part of ascorbic acid glucoside.
2. ferment skin care compositions according to claim 1, which is characterized in that comprise the following components in parts by weight: plant
6~8 parts of ferment, 3~5 parts of Crithmum maritimum stem cell extract, 1~2 part of glycyrrhetinic acid and 2~3 parts of ascorbic acid glucoside.
3. ferment skin care compositions according to claim 1 or 2, which is characterized in that comprise the following components in parts by weight: planting
7 parts of object ferment, 5 parts of Crithmum maritimum stem cell extract, 1.5 parts of glycyrrhetinic acid and 2.5 parts of ascorbic acid glucoside.
4. ferment skin care compositions according to claim 1 or 2, which is characterized in that the plant enzyme includes following
Parts by weight prepare raw material: 10~20 parts of wheat, 10~20 parts of tremella and 5~15 parts of Radix Glycyrrhizae.
5. ferment skin care compositions according to claim 4, which is characterized in that the plant enzyme includes following weight
Part prepares raw material: 15 parts of wheat, 15 parts of tremella and 10 parts of Radix Glycyrrhizae.
6. ferment skin care compositions according to claim 4, which is characterized in that the preparation of the plant enzyme include with
Lower step:
(1) wheat, tremella, Radix Glycyrrhizae of formula ratio are weighed, is mixed, is crushed, the water of 10~12 times of its dry weight is added, impregnates 1~2h,
1~2h is decocted, filtering obtains filter residue and Aqueous extracts；
(2) Aqueous extracts are concentrated into the one third of original volume at 55~60 DEG C, obtain concentrate；
(3) concentrate that filter residue that step (1) obtains, step (2) obtain is added into fermentor, is warming up to 100~120
DEG C, sterilize 30~45min, is cooled to 25~30 DEG C, and Bacillus acidi lactici is added, then saccharomyces carlsbergensis is added in 48~72h of anaerobic fermentation
Bacterium is passed through clean compressed air toward fermentor, aerobic fermentation is carried out under conditions of 25~30 DEG C, 400~800rpm/min
48~72h by filtering fermentation liquor, obtains supernatant after fermentation；
(4) supernatant that step (3) obtains is filtered using sterilizing filter, collects bacteria-free filtrate, concentrates the filtrate to substance
Long-pending one third, obtains plant enzyme.
7. ferment skin care compositions according to claim 6, which is characterized in that the preparation step of the plant enzyme
(3) inoculum concentration of Bacillus acidi lactici is the 2~4% of fermentation material total weight in, and the inoculum concentration of saccharomyces carlsbergensis bacterium is fermentation material total weight
1~3%.
8. ferment skin care compositions described in claim 1 is preparing the application in skin care item.
9. a kind of skin care item, which is characterized in that include ferment skin care compositions as claimed in claim 1 to 7.
10. skin care item according to claim 9, which is characterized in that the skin care item are toner, lotion, face cream, face
Film, eye cream or Essence.
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| CN201811337085.7A CN109394640B (en) | 2018-11-12 | 2018-11-12 | Enzyme skin care composition and application thereof |
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| CN111821237A (en) * | 2020-08-11 | 2020-10-27 | 淮阴师范学院 | Plant enzyme, preparation method thereof and application of plant enzyme in skin care products |
| CN114621881A (en) * | 2021-12-30 | 2022-06-14 | 广州君研生物科技有限公司 | Saccharomyces cerevisiae fermentation liquor and skin care product comprising saccharomyces cerevisiae fermentation liquor |
| CN115300437A (en) * | 2022-07-09 | 2022-11-08 | 黄斌斌 | Moisturizing and anti-aging skin care composition and preparation method and application thereof |
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| CN107929220A (en) * | 2017-12-01 | 2018-04-20 | 广州市韵丽生物科技有限公司 | It is a kind of that there is stem cell skin care compound for repairing anti-aging function and preparation method thereof |
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| CN111821237B (en) * | 2020-08-11 | 2022-03-04 | 淮阴师范学院 | Plant enzyme, preparation method thereof and application of plant enzyme in skin care products |
| CN114621881A (en) * | 2021-12-30 | 2022-06-14 | 广州君研生物科技有限公司 | Saccharomyces cerevisiae fermentation liquor and skin care product comprising saccharomyces cerevisiae fermentation liquor |
| CN115300437A (en) * | 2022-07-09 | 2022-11-08 | 黄斌斌 | Moisturizing and anti-aging skin care composition and preparation method and application thereof |
| CN115300437B (en) * | 2022-07-09 | 2023-10-13 | 黄斌斌 | Moisturizing and anti-aging skin care composition and preparation method and application thereof |
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| CN109394640B (en) | 2021-05-14 |
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Effective date of registration: 20230620 Address after: Room 101, No. 39 Baoying Avenue, Huangpu District, Guangzhou City, Guangdong Province, 510700, self-made unit 102 Patentee after: Guangdong Huasang Lixi Biotechnology Co.,Ltd. Address before: Room 301, 401, 39 BAOYING Avenue, Guangzhou Free Trade Zone, Guangdong 510730 Patentee before: GUANGDONG COOWAY BIOTECHNOLOGY Co.,Ltd. |