CN115300437A

CN115300437A - Moisturizing and anti-aging skin care composition and preparation method and application thereof

Info

Publication number: CN115300437A
Application number: CN202210815615.4A
Authority: CN
Inventors: 黄斌斌; 谢明宏; 唐妍娟
Original assignee: Individual
Current assignee: Individual
Priority date: 2022-07-09
Filing date: 2022-07-09
Publication date: 2022-11-08
Anticipated expiration: 2042-07-09
Also published as: CN115300437B

Abstract

The invention discloses a moisturizing and anti-aging skin care composition, a preparation method and an application thereof, wherein the skin care composition comprises the following components in parts by weight: 20 to 50 parts of hop, 20 to 40 parts of longan fruit, 10 to 30 parts of butterflybush flower, 10 to 20 parts of decursia alba and 5 to 20 parts of vetiver; the invention has remarkable capacity of inhibiting hyaluronidase, and can relieve skin inflammation injury to a certain extent when being acted on skin; moisturizing, brightening skin color and improving dark yellow skin; the components have good synergistic effect, and can better lock the moisture of the skin and reduce the water flowing, thereby achieving the effect of moisturizing.

Description

Moisturizing and anti-aging skin care composition and preparation method and application thereof

Technical Field

The invention relates to the technical field of skin care compositions, in particular to a moisturizing and anti-aging skin care composition and a preparation method and application thereof.

Background

The skin is the largest organ of the human body, has barrier, absorption, secretion, excretion, metabolism, immunity, thermoregulation and sensory functions, and the care of the skin is an essential problem in daily life. The most prominent problems of the skin are a series of skin problems caused by changes of age and seasons, such as dry skin, roughness, itching, allergy, spots, wrinkles and the like, and the skin moisturizing is very important;

the Chinese patent with the prior publication number of CN112641671A discloses a moisturizing composition and a preparation process thereof, wherein the moisturizing composition comprises the following components in parts by weight: 25-30 parts of water, 10-40 parts of glycerol, and 26 parts of glyceryl polyether: 10 to 25 portions of panthenol, 1 to 10 portions of panthenol, 0.5 to 3 portions of PCA sodium, 0.01 to 1 portion of thickening agent, 0.01 to 1 portion of neutralizing agent, 0 to 0.5 portion of essence and 0.1 to 1 portion of preservative. The preparation process is simple, the prepared moisturizing composition is low in cost and strong in operability, has excellent effects on improving wrinkle, skin hydration degree, skin roughness and skin uniformity and the like, and can improve whitening and moisturizing functions of the skin and enable the skin to have luster and elasticity after being used for a long time; wherein, the added panthenol and PCA sodium can effectively improve the defects of dull and rough complexion and lusterless skin; the added glyceryl polyether-26 can enhance the effects of moisturizing, whitening, brightening skin, removing wrinkles and resisting aging of the product;

the Chinese patent with the prior publication number of CN114010524A discloses a moisturizing composition for cosmetics, which comprises the following raw materials in percentage by mass: 5.5-7.5% of glycerol glucoside, 0.8-2% of ectoin, 3.0-5.5% of citrulline and the balance of water. The invention has the characteristics of good air permeability, short-term improvement of skin water content and immediate reduction of wrinkles;

at present, moisturizing products including the cosmetics are difficult to achieve continuous moisturizing effect, can achieve moisturizing effect only after being used continuously, and rebound quickly after stopping; therefore, the development of the cosmetic with the effects of continuously and effectively replenishing water and preserving moisture has practical significance and wide market prospect.

Disclosure of Invention

The invention aims to provide a moisturizing and anti-aging skin care composition as well as a preparation method and application thereof, and aims to solve the problems that the existing moisturizing product is difficult to realize a continuous moisturizing effect, can realize a moisturizing effect only after being continuously used and rebounds quickly after being stopped.

In order to achieve the purpose, the invention provides the following technical scheme: the moisturizing and anti-aging skin care composition comprises the following components in parts by weight: 20 to 50 parts of hop, 20 to 40 parts of longan fruit, 10 to 30 parts of butterflybush flower, 10 to 20 parts of decursia alba and 5 to 20 parts of vetiver root.

Wherein, the hop extraction part is petals, dried leaves after removing leaves and hairs, and the production place is Shandong; the longan fruit is extracted from the Guangdong province; the extract part of the buddleja officinalis is leaves, and the production place is Hubei; the root is extracted from the decursia decursivus and the origin is Sichuan; the root of vetiver is extracted from the root of vetiver and the producing area is Fujian.

A preparation method of a moisturizing and anti-aging skin care composition comprises the following steps:

step 1, raw material pretreatment, namely respectively crushing the hop, the longan fruit, the butterflybush flower, the decursia parviflora willd root and the vetiver root, and uniformly mixing the crushed hop, the longan fruit, the butterflybush flower, the decursia parviflora willd root and the vetiver root to obtain a raw material mixture for subsequent use;

step 2, adding the raw material mixture obtained in the step 1 into 85% ethanol, performing reflux extraction at 55-75 ℃ for 35-55 minutes, cooling to 42-52 ℃, and performing vacuum filtration by using a-0.05-0.1 mpa circulating water type multipurpose vacuum pump and a glass crucible to obtain a clarified mixture;

step 3, repeating the operation of the step 2;

step 4, injecting the clarified mixture obtained after the suction filtration in the step 2 and the step 3 into a rotary evaporation bottle, and performing rotary evaporation on all ethanol at the temperature of 85-100 ℃ to obtain a mixture solid;

step 5, adding the mixture solid obtained in the step 4 into an ultrasonic extractor, weighing a solvent, adding the solvent into the ultrasonic extractor, stirring at the speed of 30-45 r/min, soaking for 20-40 min, then starting to heat to 70-95 ℃, setting the ultrasonic frequency to 350-450W, setting the working frequency to 6-10 KHz, and setting the ultrasonic extraction time to 60-100 min;

step 6, after the ultrasonic extraction is finished, cooling to 45-65 ℃, and filtering to a storage tank by using filter cloth of 200-300 meshes to obtain a mixed solution I for later use;

step 7, collecting filter residues obtained after the suction filtration in the step 3, mixing, adding deionized water, adding 0.1-0.5% of plant extraction special enzyme, keeping the pH = 4.0-5.0, setting the temperature at 45-52 ℃, carrying out enzymolysis for 240-360 minutes, inactivating the enzyme at the temperature of 90-100 ℃ for 20-30 minutes after the enzymolysis is finished, and filtering by using filter cloth of 200-300 meshes to obtain an enzyme treatment mixture II for later use;

step 8, mixing the enzymolyzed filter residue with a solvent according to a mass ratio of 1-10, placing the mixture into a water bath for extraction for 150-210 minutes at 75-95 ℃ to obtain a filter residue extracting solution, cooling the filter residue extracting solution to 42-52 ℃, and filtering the filter residue extracting solution with a filter cloth of 200-300 meshes to a storage tank to obtain a mixture III for later use;

step 9, repeating the operation of the step 8, repeating the water bath extraction once to obtain a mixture IV for later use;

step 10, combining and mixing the mixtures II, III and IV, and then diluting the mixture with deionized water, wherein the dilution ratio is as follows: 1;

and 11, mixing the mixture I and the mixture V together, and concentrating by using a rotary evaporator for 0.9-1.0 g/ml to obtain a concentrated mixture, thus obtaining the moisturizing and anti-aging skin care composition.

Wherein, in the step 1, the pulverized hops, longan fruits, butterflybush flowers, decursinia decurrens roots and vetiver roots are filtered through a sieve of 80 to 250 meshes.

In the step 2, the mass fraction ratio of the raw material mixture to the ethanol is 1.

Wherein, in the step 5, the mass ratio of the mixture solid to the solvent is 1.

In the step 5, the solvent is one or more of butanediol, propylene glycol, glycerol and 1, 2-hexanediol, deionized water is added into the solvent, and the mass ratio of the solvent to the deionized water is 1.

In the step 7, the mass ratio of the filter residue to the deionized water is 1.

In step 7, the plant extraction special enzyme is composed of 1 ten thousand U/g cellulase, 1 ten thousand U/g hemicellulase, 3 ten thousand U/g glucanase, 1 ten thousand U/g pectinase and 1 ten thousand U/g papain.

Wherein, in step 7, in step 8, the solvent is one or more of butylene glycol, glycerol, and 2, 3-butylene glycol.

The application of the moisturizing and anti-aging skin care composition is that one of lotion, essence, cream, eye cream and emulsion which are commonly used in the moisturizing and anti-aging skin care composition is mixed to obtain the skin care product with the moisturizing and anti-aging effects, and the addition amount of the moisturizing and anti-aging skin care composition is 1-10% of the weight of the skin care product.

Among them, solvent butanediol-producing Japan Kabushiki Kaisha (DAICEL CORPORATION); 2, 3-butanediol producing region Shandong Qing Dazhixing Biotech Co., ltd; the glycerol production area: tokyo brown (zhang ) limited; SKC PICGLOBAL co, ltd, propylene glycol production site; deionized water: EDI ultrapure water self-production; plant extract special enzyme (Nanning Dong Henghuadao Biotech, inc.).

Wherein, the preparation equipment is as follows: an ultrasonic extractor (model: SCIENTZ-5T, available from Ningbo Xinzhi Biotechnology Co., ltd.), a circulating water type multi-purpose vacuum pump (model: SHB-111, zhengzhou Changcheng Kogyo Co., ltd.), a glass crucible (with a pore diameter of 16-30 um, about 30 mL), a CF-44 sealed swinging type pulverizer (finished product fineness: 80-250 mm), a rotation speed of 25000r/min, a Chuanli medicinal material instrument factory in Wenling City of Zhejiang province, and a rotary evaporator (model: RE-52A, zhengzhou Yao instrument science and technology Co., ltd.).

Compared with the prior art, the invention has the beneficial effects that:

the moisturizing and anti-aging skin care composition prepared by the invention has remarkable capacity of inhibiting hyaluronidase, and can relieve skin inflammation injury to a certain extent when acting on skin; moisturizing, brightening skin color and improving dark yellow skin;

the moisturizing and anti-aging skin care composition prepared by the invention can effectively improve the water content of the skin when being applied to the mask, wherein the components have good synergistic effect, can better lock the moisture of the skin and reduce the water flowing of the moisture, thereby achieving the effect of moisturizing;

the moisturizing and anti-aging skin care composition prepared by the invention can reduce wrinkles, improve the skin roughness and smoothness and inhibit the activity of collagenase and elastase when being applied to the cream.

Drawings

FIG. 1 is a graph showing the rate of change of skin moisture content at various time points after application of the product in application example 1 of the present invention relative to that before application.

Detailed Description

The technical solution of the present invention is further described in detail with reference to the specific embodiments, but the present invention is not limited thereto, and any limited number of modifications made by anyone within the scope of the present invention are still within the scope of the present invention. It should be noted that the raw materials used in the technical scheme provided by the present invention are all purchased from the market by conventional means except for special instructions.

Example 1

The moisturizing and anti-aging skin care composition comprises the following components in parts by weight: 30 parts of hops, 24 parts of longan fruits, 18 parts of butterflybush flowers, 13 parts of decursia alba roots and 15 parts of vetiver roots.

Example 2

The moisturizing and anti-aging skin care composition comprises the following components in parts by weight: 50 parts of hops, 20 parts of longan fruits, 12 parts of butterflybush flowers, 12 parts of decursia alba roots and 6 parts of vetiver roots.

Example 3

The moisturizing and anti-aging skin care composition comprises the following components in parts by weight: 20 parts of hops, 40 parts of longan fruits, 25 parts of butterflybush flowers, 10 parts of decursia alba roots and 5 parts of vetiver roots.

Example 4

The moisturizing and anti-aging skin care composition comprises the following components in parts by weight: 25 parts of hops, 25 parts of longan fruits, 30 parts of butterflybush flowers, 13 parts of decursia alba roots and 7 parts of vetiver roots.

Example 5

The moisturizing and anti-aging skin care composition comprises the following components in parts by weight: 40 parts of hops, 28 parts of longan fruits, 15 parts of butterflybush flowers, 11 parts of decursia alba roots and 6 parts of vetiver roots.

Preparation example

step 2, adding the raw material mixture obtained in the step 1 into 85% ethanol, performing reflux extraction at 60 ℃ for 40 minutes, cooling to 48 ℃, and performing vacuum filtration by using a-0.01 mpa circulating water type multipurpose vacuum pump and a glass crucible to obtain a clarified mixture;

step 3, repeating the operation of the step 2;

step 4, injecting the clarified mixture obtained after the suction filtration in the step 2 and the step 3 into a rotary evaporation bottle, and performing rotary evaporation on all ethanol at the temperature of 90 ℃ to obtain a mixture solid;

step 5, adding the solid mixture obtained in the step 4 into an ultrasonic extractor, weighing a solvent, adding the solvent into the ultrasonic extractor, stirring at the speed of 40 revolutions per minute, soaking for 30 minutes, then starting to heat to 85 ℃, setting the ultrasonic frequency to 400W, setting the working frequency to 8KHz, and setting the ultrasonic extraction time to 90 minutes;

step 6, after the ultrasonic extraction is finished, cooling to 55 ℃, and filtering to a storage tank by using 250-mesh filter cloth to obtain a mixed solution I for later use;

step 7, collecting filter residues obtained after the suction filtration in the step 3, mixing, adding deionized water, adding 0.3% of plant extraction special enzyme, setting the pH =4.0, carrying out enzymolysis at the temperature of 48 ℃ for 300 minutes, inactivating the enzyme at the temperature of 95 ℃ for 25 minutes after the enzymolysis is finished, and filtering with 250-mesh filter cloth to obtain an enzyme treatment mixture II for later use;

step 8, mixing the enzymolyzed filter residue with a solvent according to a mass ratio of 1 to 15, putting the mixture into a water bath at 85 ℃ for extraction for 200 minutes to obtain a filter residue extracting solution, cooling the filter residue extracting solution to 48 ℃, and filtering the filter residue extracting solution with a filter cloth of 250 meshes to a storage tank to obtain a mixture III for later use;

step 10, combining and mixing the mixtures II, III and IV, and then diluting the mixture with deionized water, wherein the dilution ratio is as follows: 1, obtaining a mixture V;

and 11, mixing the mixture I and the mixture V together, and concentrating by using a rotary evaporator to obtain a concentrated mixture, namely obtaining the moisturizing and anti-aging skin care composition.

Comparative example 1

Compared with the example 1, the hop is replaced by the deionized water, and the content of other components and the process are not changed.

Comparative example 2

Compared with the example 1, the longan fruit is replaced by the hop, and the content of other components and the process are not changed.

Comparative example 3

Compared with example 1, flos Buddlejae and radix Linderae Caudatae are replaced by arillus longan and flos Lupuli, and other components and processes are unchanged.

Comparative example 4

Compared with the example 1, the longan fruit beverage does not contain buddleja officinalis, is replaced by longan fruits, and does not change the content of other components and the process.

Comparative example 5

Compared with the example 1, the butterflybush flower and the vetiver root are replaced by the longan fruit and the decurrent bugle herb root, and the content of other components and the process are not changed.

Examples of the experiments

In order to prove the technical points of the technical scheme provided by the application, the experimental data of examples 1-5 and comparative examples 1-5 provided by the application are given below;

1. in vitro assay-assay for inhibition of hyaluronidase Activity

1.1, test principle:

hyaluronidase (HAase) is capable of indiscriminately hydrolyzing sodium hyaluronate to yield the product β -N-acetylglucosamine, which can condense with borate under alkaline conditions to produce a chromogen that develops color with p-dimethylaminobenzaldehyde under acidic conditions. The test inhibits hyaluronidase activity by adding an analyte, which limits its enzymatic reaction. In view of the conjugation effect of the generation system, the inhibition efficiency of the analyte on hyaluronidase is reflected by taking the absorption wavelength at the maximum absorbance as the optimal selection wavelength and examining the change of the absorbance.

1.2 Experimental procedures

(1) Reagent set-up

Acetic acid buffer (pH 3.5), 0.5% sodium hyaluronate, 100U/ml hyaluronidase, 0.1% sodium hydroxide, 0.1% sodium borate, 1% centella asiatica extract, 0.1mol/L p-dimethylaminobenzaldehyde

(2) A, receiving a sample: the test examples 1 to 5 and the comparative examples 1 to 5 were diluted with acetic acid buffer solutions to 1% samples, respectively; respectively adding 0.5ml of hyaluronidase, 0.5ml of sample, 0.5ml of sodium hyaluronate, 0.1ml of sodium hydroxide and 0.5ml of sodium borate into a test tube, fully shaking up, and dripping 1ml of 0.1mol/L p-dimethylaminobenzaldehyde into the test tube; the cells were incubated at 37 ℃ for 30min in a water bath, transferred to a 96-well plate and the absorbance measured at 582 nm.

(3) A test sample blank control: respectively adding 0.5ml of acetic acid buffer solution, 0.5% of sodium hyaluronate, 100U/ml of hyaluronidase, 0.1% of sodium hydroxide and 0.1% of sodium borate into a test tube, fully shaking, dripping 1ml of 0.1mol/L p-dimethylaminobenzaldehyde, preventing 30min at 30 ℃, transferring to a 96-well plate, and measuring the light absorption value at 582 nm.

(4) B negative control: respectively adding herba Centellae extract 0.5ml, hyaluronidase 0.5ml, sample 0.5ml, sodium hyaluronate 0.5ml, sodium hydroxide 0.1ml, and sodium borate 0.5ml into test tube, shaking, dripping p-dimethylaminobenzaldehyde 1ml and 0.1mol/L, preventing at 30 deg.C for 30min, transferring to 96-well plate, and measuring light absorption value at 582 nm.

(5) B negative blank control: respectively adding 0.5ml of acetic acid buffer solution, 0.5% of sodium hyaluronate, 100U/ml of hyaluronidase, 0.1% of sodium hydroxide and 0.1% of sodium borate into a test tube, fully shaking, dripping 1ml of 0.1mol/L p-dimethylaminobenzaldehyde, preventing 30min at 30 ℃, transferring to a 96-well plate, and measuring the light absorption value at 582 nm.

1.3, the calculation formula of the hyaluronidase activity inhibition rate (1) is as follows:

1.4, the results of the test data are shown in Table 1 for the hyaluronidase Activity inhibition ratio

TABLE 1 inhibition of hyaluronidase Activity

1.5, conclusion

As shown in Table 1, the hyaluronidase inhibition rates of examples 1-5 and comparative examples 1-5 are about 52.9% or more and about 21.5% or less, and the negative control is compared with examples 1-5, and the hyaluronidase inhibition rates of examples 1-5 are about 2 times that of the blank control; the higher the inhibition rate is, the better the anti-allergic and anti-inflammatory effects are. As can be seen from table 1, the moisturizing skin care composition of the present invention has a significant ability to inhibit hyaluronidase, which indicates that it has a good anti-allergic and anti-inflammatory activity, and can relieve skin inflammation injury to a certain extent when acting on skin.

2. In vitro assay-free radical scavenging assay

Examples 1-5 and comparative examples 1-5 were validated with reference to the Shanghai society for daily chemical industry, group Standard T/SHRH 006-2018 cosmetic-free radical (DPPH) scavenging test method.

2.1, test principle:

1, 1-diphenyl-2-trinitrophenylhydrazine (DPPH for short) is a stable long-life free radical, and the ethanol solution of the free radical is dark purple and has strong absorption near 517 nm. In the presence of the free radical scavenger, the DPPH ethanol solution absorbs less light due to its one-electron pairing. The degree of discoloration of the DPPH ethanol solution is in a linear relationship with the number of electrons accepted by the DPPH ethanol solution, so that the capability of the test sample for scavenging free radicals can be evaluated, namely, the higher the scavenging rate is, the stronger the capability of the test sample for scavenging free radicals for resisting oxidation is shown.

2.2 Experimental procedures

(1) Reagent preparation

Ethanol solution of DPPH: weighing 40mg of DPPH, adding absolute ethyl alcohol to dissolve, and fixing the volume in a 1000mL volumetric flask, wherein the DPPH concentration is prepared to be 40mg/L. Storing in dark at low temperature for 5 days.

Liquid to be detected: the moisturizing and skin care compositions of examples 1-5 and comparative examples 1-5 were diluted to a concentration of 1.0% with a phosphate buffer.

Positive control sample: vitamin C was diluted to a concentration of 1.5X 10-4g/l with distilled water.

(2) Experimental methods

A1 test sample group: adding 1.0mL of DPPH test solution with the mass concentration of 40mg/L and 1.0mL of sample to be tested into a test tube, supplementing the total volume to 3mL by absolute ethyl alcohol, shaking up, reacting for 30min in a dark place, taking 1.0mL, transferring into a 96-well plate, and measuring the absorbance at the wavelength of 517 nm;

a2 blank control sample group: adding 2mL of absolute ethyl alcohol and 1.0mL of a sample to be detected into a test tube, shaking up, carrying out a dark reaction for 30min, and transferring 1.0mL to a 96-well plate to measure absorbance at the wavelength of 517 nm;

a3 negative control group: adding 1.0mL of DPPH test solution with the mass concentration of 40mg/L and 2mL of absolute ethyl alcohol into a test tube, shaking up, carrying out a dark reaction for 30min, taking 1mL, transferring the solution into a 96-well plate, and measuring the absorbance at the wavelength of 517 nm;

positive control group: adding 1.0mL of DPPH test solution with the mass concentration of 40mg/L and 1.0mL of positive control sample into a test tube, supplementing the total volume to 3mL by absolute ethyl alcohol, shaking up, reacting for 30min in a dark place, taking 1.0mL, transferring into a 96-well plate, and measuring the absorbance at the wavelength of 517 nm.

2.3, detection result

(1) And the calculation formula of the DPPH free radical clearance rate (2) of the sample is as follows:

(2) And the test data results are shown in table 2.

Table 2 data results

2.4, conclusion

From the test results, examples 1 to 5 showed outstanding ability to scavenge DPPH radicals, and the clearance rate was about 80% or more. Compared with the examples 1 to 5, the comparative examples 1 to 5 have poor effect, and the clearance rate is about less than 45 percent; the technical schemes of examples 1-5 have the effect of clearing rate which is about equivalent to that of the positive control sample with the concentration of 1.5 multiplied by 10 < -4 > g/l, but is about twice that of the comparative examples 1,2, 3 and 5; the moisturizing and skin care composition disclosed by the invention can moisturize water and moisturize, brighten skin color and improve dark and yellow skin.

Application example 1

The formula of the essence facial mask liquid with the moisturizing and anti-aging effects is respectively added with the moisturizing and skin-care composition materials of the example 1 (A) and the comparative example 1 (B) and the facial mask matrix without the examples and the comparative examples, and tests are carried out; the test formulations are given in table 3 below:

TABLE 3A facial mask liquid with moisturizing effect

Production process

(1) Weighing the raw materials of the group A, adding the raw materials into an emulsifying pot, stirring at the speed of 35-45 r/min, homogenizing at the medium speed of 3000r/min for 2-3 minutes until the raw materials are semitransparent viscous liquid, stirring and heating to 83-85 ℃, and keeping the temperature for 15-20 minutes until the raw materials are transparent liquid;

(2) Cooling, stirring at the speed of 30-40 r/min, and vacuumizing to-0.03 mpa;

(3) Cooling to below 45 ℃, weighing the raw materials of the group B, adding the raw materials into an emulsifying pot, and stirring at the speed of 30-40 r/min for 5-8 minutes until the mixture is yellowish transparent viscous liquid;

(4) Weighing the raw materials of the group C, uniformly mixing in a container, preheating in a water bath kettle at 75-78 ℃ for dissolving and transparent, adding into an emulsifying kettle, stirring at the speed of 30-40 r/min for 8-15 minutes, and homogenizing at the speed of 1000r/min for 1-3 minutes to obtain yellowish transparent viscous liquid;

(5) Weighing the raw materials in the group D, adding the raw materials into an emulsifying pot, and stirring the mixture for 5 to 8 minutes at the speed of 30 to 40r/min until the mixture is yellowish transparent viscous liquid;

(6) And (4) the physical and chemical indexes are qualified, and the mixture is filtered by using 200-400 meshes of filter cloth, placed in a storage tank and kept stand for 24H, and then the microorganism is detected.

The internal control standard of the essence facial mask liquid with the moisturizing and anti-aging effects is as follows:

appearance: a yellowish transparent viscous liquid;

odor: rose water fragrance;

pH:5.5～6.8

viscosity: the 2# rotating speed is more than or equal to 800mpa at 25 ℃;

stability: cold resistance: the temperature is kept for 24h at (-8 +/-2) ℃, and no obvious difference exists after the temperature is restored to the room temperature.

Heat resistance: the temperature is kept at (40 +/-1) ℃ for 24 hours, and no obvious difference exists between the temperature after being restored to the room temperature and before the test.

Microorganisms: total number of colonies: less than or equal to 10FCU/g;

total number of molds and yeasts: less than or equal to 10CFU/g;

heat-resistant coliform group bacteria: cannot be detected;

and (4) staphylococcus aureus: cannot be detected;

pseudomonas aeruginosa: cannot be detected;

heavy metals: mercury is less than or equal to 1mg/kg; lead is less than or equal to 10mg/kg; arsenic is less than or equal to 2mg/kg; cadmium is less than or equal to 5mg/kg.

The matching degree of the facial mask liquid and plant cotton fibers such as Xinjiang cotton fibers, folium artemisiae argyi fibers, banana fiber cotton and the like reaches 99 percent after 90-day test.

And (3) carrying out a moisturizing performance test on the essence facial mask liquid with the moisturizing and anti-aging effects:

the tests of the moisture content of the skin before use and after 1h, 2h, 3h and 4h of the product after use of the essence facial mask liquid of the facial mask matrix in example 1 and comparative example 1 are evaluated by a 4-h human body moisturizing test, and the larger the moisture content value of the skin is, the higher the moisture content of the skin is.

Volunteers require:

the number of volunteers: 25, 6 per group, female;

the cosmetic has no history of allergy, the cosmetic is stopped 3 days before use, patients without skin disease, women in pregnancy or lactation period have no health;

age: 25-35 years old;

test area: forearm flexor;

and (3) testing period: 4H;

testing an instrument:

skin moisture content tester Corneometer (CM 825, courage and Khazaka, germany).

Positive control sample:

0.05% sodium hyaluronate (molecular weight: 1.3-1.35X 105, shandong Anhua biological medicine Co., ltd.).

And (3) testing process:

(1) Controlling the indoor temperature to be 20-22 ℃; relative humidity 50. + -.5% RH

(2) 24 persons are divided into 4 groups, 6 persons are in each group, the group A is in example 1, the group B is in comparative example 1, the mask substrate is a substance which does not contain any moisturizing, moisturizing and anti-aging skin care composition, and the sodium hyaluronate is a positive control group.

(3) Before use, the test skin area is tested, recorded as 0h data and marked.

(4) And cutting the test sample into pieces of 4cm by 4cm, pasting the test sample in a test area before the test sample is marked for use by a subject, and after 20min of pasting, removing a mask, massaging the test sample until the test sample is absorbed, and performing return visit.

(4) Applying for 20min, removing the mask, massaging to the 1 st, 2 nd, 3 rd and 4 th hours after absorption, and retesting. The test was carried out with the area of the sample-free mask as a blank.

The test result calculation formula is as follows:

initial value: refers to the base value (data before use, 0 h) when no product is used.

Mean value

Where x = individual parameter measurement, n = number of valid data

Difference from initial value = xT after use-xT before use

And (3) testing results:

the results of the skin moisture content test before the product was used and after the product was used for 1h, 2h, 3h and 4h are shown in table 4 and fig. 1.

Table 4 mean values and statistics of skin moisture content before use, after 1h, 2h, 3h, 4h of product (n = 24);

TABLE 4

And (4) conclusion:

as can be seen from table 4 and the data in fig. 1, in the embodiment 1 of the moisturizing and anti-aging skin care composition provided by the invention, the moisturizing and anti-aging skin care composition has a good moisturizing effect when applied to an essence facial mask liquid with moisturizing and anti-aging effects; after the composition is used for 1 hour, the moisture content of the skin reaches a peak value, the moisture content is obviously higher than that of a blank group without the mask and is also higher than that of a comparative example 1 and a positive control sample, so that the moisture-preserving and anti-aging skin-care composition disclosed by the invention can effectively improve the moisture content of the skin when being applied to the mask, the components have good synergistic effects, the moisture-preserving effect of the mask can be influenced by the absence of any component, and the technical scheme can better lock the moisture of the skin and reduce the water flowing of the moisture, so that the moisture-preserving effect is achieved.

Applications 2

A moisturizing and moisturizing cream is prepared by respectively adding 5% of moisturizing and skin-care composition materials of example 5 (A) and comparative example 5 (B) to a cream base, and performing a test without adding the moisturizing and skin-care composition materials of the example and the comparative example; test formulations table 5 is as follows:

table 5 cream with moistening, moisturizing and moisture-keeping functions

The production process comprises the following steps:

1) Weighing the raw materials of the group A, adding the raw materials into an oil pan, stirring at the speed of 35r/min, heating to 78-83 ℃, dissolving and transparent for later use;

2) Weighing the raw materials in the group B, adding the raw materials into an emulsifying pot, stirring at the speed of 35r/min, and heating to 83-85 ℃ for dissolving and transparency;

3) Weighing the raw materials in the group C, uniformly mixing the raw materials in a container until viscous liquid is added into an emulsifying pot, and stirring the mixture for 15 minutes at a speed of 65-70 r/min until the mixture is semitransparent liquid;

4) Pumping the dissolved raw materials of the group A into an emulsifying pot by a-0.05 mpa vacuum pump, stirring at the speed of 45-55 r/min, and homogenizing at the high speed of 30000r/min for 15 minutes to obtain milk white liquid;

5) Weighing the raw materials of the group D, adding the raw materials into an emulsifying pot, stirring at the speed of 35-45 r/min, and homogenizing at the speed of 5000r/min for 1 minute to obtain viscous emulsion;

6) Cooling, vacuumizing to-0.03mpa, and stirring at 35-38 r/min;

7) Cooling to below 45 ℃, weighing the raw materials of the group E, adding the raw materials into an emulsifying pot, and stirring at the speed of 35-38 r/min for 15 minutes until white emulsion is obtained;

8) Respectively weighing the F group of raw materials, adding the raw materials into an emulsifying pot, and stirring the mixture for 10 minutes at the speed of 35 to 38r/min until white emulsion is formed;

9) Weighing raw materials of G, adding the raw materials into an emulsifying pot, and stirring the mixture for 10 minutes at the speed of 35 to 38r/min until white emulsion is formed;

10 Discharging after the physicochemical index is qualified.

Wherein, the physical and chemical indexes are as follows:

appearance: white brightening emulsion

pH:5.5～6.8

Odor: fragrance of flower water;

stability and cold resistance: keeping the temperature at (-8 +/-2) ℃ for 24h, and no layering phenomenon exists after the room temperature is recovered;

stability and heat resistance: keeping the temperature at (40 +/-1) ℃ for 24 hours, and no layering phenomenon exists after the room temperature is recovered;

centrifugal examination: 2000r/min,20min, no delamination;

microorganisms: total number of colonies: less than or equal to 10FCU/g;

total number of molds and yeasts: less than or equal to 10CFU/g;

heat-resistant coliform group bacteria: cannot be detected;

staphylococcus aureus: cannot be detected;

pseudomonas aeruginosa: cannot be detected;

Wherein, the particularly beautiful soft nano liquid consists of 0.3 to 1.0 percent of polyglycerol-10 heptastearate, 10.0 to 15.0 percent of glycerol, 15.0 to 25.0 percent of butanediol, 18.0 to 22.0 percent of isododecane, 42.0 to 48.0 percent of caprylic/capric triglyceride and 3.0 to 5.0 percent of deionized water, and is obtained by a low-temperature or normal-temperature dispersion emulsification technology, and the use purpose is to enhance the skin moistening property and the water locking property.

The wrinkle reducing effect experiment is carried out on the cream with the functions of moistening, moisturizing and preserving moisture:

the efficacy of creams a, B, a base to reduce wrinkles and improve skin roughness, lubrication was evaluated by a 4-week human test.

(1) And testing pretreatment: 15 volunteers with wrinkles and healthy and undamaged skin with the age of 38-48 are selected to respectively test the cream A, the cream B and the matrix, and 5 persons in each group. The support person applies the test sample twice daily to the face. During the experiment, the subject was not able to apply any other cosmetic product at the experimental site. The test subjects performed the test after 0, 1,2, 3, 4 weeks of continuous use of the test samples.

(2) And the test method comprises the following steps: the skin condition was measured using a skin wrinkle tester VISIOMETER SV600 at weeks 0, 1,2, 3, and 4, respectively, and the test site was the face. The test parameter is skin roughness Rt which is the difference value of dermatoglyph from the highest peak to the lowest valley and is called as Rl in the software of the instrument; average roughness RZ is the arithmetic average of 5 consecutive segments of the same length, called R3 in the software of the instrument, the larger the values of R1 and R3, the more severe the wrinkles, indicating a worse roughness and lubrication of the skin.

(3) And calculating a formula:

skin roughness R1 variation = R1 _{The fourth side} -R1 _{Initial value} ；

Skin roughness change rate R1 change rate = [ (R1) _{The fourth side} -R1 _{Initial value} )/R1 _{Initial value} ]×100％；

Skin roughness average R3 variation = R3 _{The fourth side} -R3 _{Initial value} ；

Skin roughness change rate R3 change rate = [ (R3) _{The fourth side} -R3 _{Initial value} )/R3 _{Initial value} ]×100％；

Initial value: refers to the basal value when no product was used (data before use, 0 weeks);

the test results for specific R1 are shown in table 6, and the test results for R3 are shown in table 7:

TABLE 6 variation in skin roughness R1 (in mm) after use of cream A, B, base product

TABLE 7 variation in average roughness of skin R3 (in mm) after use of creams A, B, base product

And (4) conclusion:

table 6 shows the change of the skin roughness R1 around the products using creams a, B and a base, and the experimental results in table 6 show that the product using cream a can significantly reduce the difference between the highest peak and the lowest valley of the dermatoglyph, i.e., significantly reduce the skin roughness, and that the skin roughness can be reduced by more than 21.42% after the product using cream a is around the skin. Table 7 shows the change of the average roughness R3 of the skin around the cream a, B and base products, and the results of the experiment in table 7 show that the average roughness of the skin can be reduced by more than 54.79% after the cream a is used for four weeks. As can be seen from the results in tables 6 and 7, the moisturizing and anti-aging skin care composition of the present invention can reduce wrinkles, improve skin roughness and smoothness, and inhibit collagenase activity and elastase activity when added to the cream of the present invention.

All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Claims

1. A moisturizing and anti-aging skin care composition, which is characterized in that: the skin care composition comprises the following components in parts by weight: 20 to 50 parts of hop, 20 to 40 parts of longan fruit, 10 to 30 parts of butterflybush flower, 10 to 20 parts of decumbent bugle herb root and 5 to 20 parts of vetiver root.

2. The preparation method of the moisturizing and anti-aging skin care composition is characterized by comprising the following steps:

step 3, repeating the operation of the step 2;

step 5, adding the solid mixture obtained in the step 4 into an ultrasonic extractor, weighing a solvent, adding the solvent into the ultrasonic extractor, stirring at the speed of 30-45 rpm, soaking for 20-40 minutes, then starting to heat to 70-95 ℃, setting the ultrasonic frequency to 350-450W, setting the working frequency to 6-10 KHz, and setting the ultrasonic extraction time to 60-100 minutes;

step 9, repeating the operation of the step 8, and repeating the water bath extraction once to obtain a mixture IV for later use;

3. The moisturizing and anti-aging skin care composition as claimed in claim 2, wherein in step 1, the pulverized hops, longan fruits, butterflybush flowers, decursinia alba and vetiver roots are filtered through a sieve of 80-250 meshes.

4. The moisturizing and anti-aging skin care composition as claimed in claim 2, wherein in the step 2, the mass fraction ratio of the raw material mixture to ethanol is 1.

5. The moisturizing and anti-aging skin care composition as claimed in claim 2, wherein in the step 5, the mass ratio of the mixture solid to the solvent is 1.

6. The moisturizing and anti-aging skin care composition according to claim 2, wherein in the step 5, the solvent is one or more of butanediol, propylene glycol, glycerol and 1, 2-hexanediol, and deionized water is added to the solvent, and the mass ratio of the solvent to the deionized water is 1.

7. The moisturizing and anti-aging skin care composition according to claim 2, wherein in the step 7, the mass ratio of the filter residue to the deionized water is 1.

8. The moisturizing anti-aging skin care composition of claim 2, wherein in step 7, the plant extract specific enzymes consist of 1 ten thousand U/g cellulase, 1 ten thousand U/g hemicellulase, 3 ten thousand U/g glucanase, 1 ten thousand U/g pectinase and 1 ten thousand U/g papain.

9. The moisturizing and anti-aging skin care composition as claimed in claim 2, wherein in step 7, in step 8, the solvent is one or more of butylene glycol, glycerin and 2, 3-butylene glycol.

10. The application of the moisturizing and anti-aging skin care composition as claimed in any one of claims 1 to 9, wherein one of lotion, essence, cream, eye cream and emulsion commonly used in the moisturizing and anti-aging skin care composition is mixed to obtain a skin care product with moisturizing and anti-aging effects, and the moisturizing and anti-aging skin care composition is added in an amount of 1 to 10% by weight of the skin care product.