CN114431038A - Root division research device for different functional root systems of wheat and test arrangement method - Google Patents

Root division research device for different functional root systems of wheat and test arrangement method Download PDF

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Publication number
CN114431038A
CN114431038A CN202210218810.9A CN202210218810A CN114431038A CN 114431038 A CN114431038 A CN 114431038A CN 202210218810 A CN202210218810 A CN 202210218810A CN 114431038 A CN114431038 A CN 114431038A
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China
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root
wheat
planting
partition plate
hole
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CN202210218810.9A
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杨习文
吴寅
臧贺藏
张均
贺德先
周苏玫
宋淼
黄源
龚璞
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Henan Agricultural University
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Henan Agricultural University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G9/00Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
    • A01G9/02Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
    • A01G9/028Multi-compartmented pots
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/20Cereals

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Botany (AREA)
  • Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)

Abstract

The invention belongs to the technical field of root division research of wheat crops, and particularly discloses a root division research device for different functional roots of wheat and a test arrangement method.

Description

Root division research device for different functional root systems of wheat and test arrangement method
Technical Field
The invention belongs to the technical field of root division research of wheat crop roots, and particularly relates to a root division research device and a test arrangement method for different functional root systems of wheat.
Background
The root system of the wheat belongs to a fibrous root system and is divided into a primary root and a secondary root; the primary root can grow to 150-200 cm underground, is a main period for the primary root to play a role from seedling emergence to jointing, has a large role in the later period (after grouting), and particularly has special significance for utilizing deep soil moisture under the later-period drought condition; according to the research, the primary root is cut at any time in the whole life of wheat, and the wheat is seriously influenced. This shows that the plants have activity after the secondary rooting and before the maturation, except for the water and nutrient which are independently supplied to the plants before tillering by the primary eradication; in addition, the primary root grows stably and can maintain the minimum requirement of wheat growth under the adverse environment condition, which shows that the primary root is closely related to the stable yield of wheat. The secondary roots are mostly concentrated in a plough layer of 20-30 cm, the occurrence time of the secondary roots is late, after the joints are pulled out, the further differentiation and development of the spikes, the vigorous growth of stems and leaves, the formation and maturation of the sub-grains and the like mainly depend on the activities of the secondary roots. The secondary roots have long rooting time, large number (large root quantity) and strong function, are the main part of the root system and have close relation with high yield. Then, how much the function difference between the two is, how much each contributes to high and stable yield of wheat, and further research is needed.
The primary root and the secondary root have obvious physiological and ecological difference, and at present, the research on the functional difference of the primary root and the secondary root is only reported and is mainly limited by the defects of a research method and a root dividing device; the existing root dividing test technology only simply divides the root system of a plant into two parts, the functions of the two parts of the root system exerted on the growth of the plant are actually the same, and the simple root dividing technology cannot meet the research of the difference of the primary root function and the secondary root function.
Disclosure of Invention
The invention aims to provide a device for researching root division of different functional roots of wheat and a test arrangement method, provides technical support for researching different root function differences of the wheat, provides a technical method for further researching different special physiological functions of primary roots and secondary roots of the wheat, and compared with the existing root division technology, the device can easily separate the primary roots and the secondary roots of the wheat, process the primary roots and the secondary roots in respective growth environments according to research requirements, and finally clearly describe the differences in physiological functions of the primary roots and the secondary roots and the like.
In order to achieve the purpose, the invention adopts the technical scheme that:
the utility model provides a different function root system of wheat divides root research device, is including planting the basin, be equipped with the division board in planting the basin, the space that the division board will be planted in the basin is equallyd divide for two and plants the chamber, and two soil or planting environment that the chamber convenient to use is different are planted to two, are convenient for form the contrast, are favorable to dividing going on of root research, be equipped with one row of through-hole apart from top 4 cm's position on the division board, the setting of through-hole is convenient for the primary root of connecting on the terrestrial stem of wheat and is passed the baffle and grow in one of them separate intracavity, with the separate research of primary root and secondary root, through-hole upper end earthing 1 ~ 2cm, the basin is convenient for water along the surplus runoff, prevents.
Furthermore, the through hole is an inclined hole forming an angle of 45 degrees with the horizontal plane, a guide pipe is movably inserted in the through hole, the included angle between the guide pipe and the partition plate is 45 degrees, the side wall of the guide pipe is provided with a through opening, the inclined hole is arranged to match the growth direction of the underground stem, the underground stem and the primary root can smoothly penetrate through the through hole conveniently, the influence on the underground stem and the primary root is minimum without changing the growth direction, the guide pipe is arranged in a mode that the side wall is provided with the through opening, the underground stem and the primary root can smoothly penetrate into the guide pipe without damaging the primary root and the underground stem, and the guide pipe is arranged to conveniently guide the primary root and the underground stem to penetrate through the inclined hole.
Furthermore, plant the basin and be square, the division board is located and plants the basin middle and with planting basin lateral wall parallel arrangement.
Further, the square has a width of 20cm and a length and a height of 30cm, and the dimensions are selected to maximally simulate a density of 270 ten thousand per hectare of wheat crop seedlings.
A test arrangement method of a wheat root division research device with different functional roots comprises the following steps:
(1) selecting wheat seeds and growing the wheat seeds in a seedling growing pond, wherein the row spacing is 20cm and the plant spacing is 2cm during sowing;
(2) transplanting the seedlings which are robust, uniform and consistent in growth, complete and undamaged in root systems and about 1cm in stem length in the ground, placing the underground stems and the primary roots in a guide pipe with an opening to penetrate through a partition plate through hole to enable the underground stems of the wheat to be located in the through hole, respectively locating the primary roots and the overground parts at two sides of a partition plate, filling soil and watering, embedding a planting pot in the field to complete test arrangement, and enabling the seedlings to grow secondary roots after the wheat is tilled and to be located at the other side of the partition plate, where the primary roots are wanted to be located.
Furthermore, the planting pot is made of plastic, and the planting density of a field test is 270 thousands of seedlings per hectare.
The invention has the advantages that: the invention utilizes the partition plate to distribute the root systems with different functions in two different growth spaces, and the cavities on the two sides can be treated by different water and fertilizer, thereby exploring the response of the root systems with different functions to the treatment of different water and fertilizer, not only separately researching the root systems with different functions, but also approaching the field environment as much as possible, simulating the density of field growth, and being more tightly combined with production. The method comprises the following specific steps:
1. the seedling culture method of the invention designs the seeding depth to be 5cm, and the special biological characteristics of wheat are utilized to form the underground stem with the length of about 1cm, thereby not only not influencing the normal growth and development of the wheat, but also separating the primary root and the secondary root according to the space distribution difference of the primary root and the secondary root;
2. the invention integrally designs a test device for separating root systems with different functions of wheat, the root systems with different functions are distributed in two different growth spaces by utilizing a partition plate, cavities on two sides can be treated by using different water and fertilizers, and the response of the root systems with different functions to the treatment of different water and fertilizers is further explored;
3. the matched guide pipe with the opening in the transplanting process can effectively protect primary roots in the root-penetrating transplanting process, is convenient for seedling transplanting operation, and improves the working efficiency;
4. the 45-degree perforation on the partition board in the test device is beneficial to connecting the primary root and the overground part of the underground stem to be in a natural growth state as far as possible when the underground stem penetrates through the partition board, so that the normal growth of wheat and the formation of strong seedlings are facilitated; in addition, the inclined holes are favorable for seedling transplanting operation.
Drawings
FIG. 1 is a schematic diagram of the present invention.
Fig. 2 is a schematic view of the structure of the catheter of the present invention.
Fig. 3 is a reference view showing a state of use of the catheter in the present invention.
Detailed Description
As shown in the figure, the device for researching the root division of the different functional root systems of the wheat comprises a planting pot 1, a partition plate 2 is arranged in the planting pot, the partition plate 2 divides the space in the planting pot into two planting cavities, the two planting cavities are convenient to use different soil or planting environments so as to form comparison and be beneficial to the research of the root division, a row of through holes 21 are formed in the position, 4cm away from the top, of the partition plate 2, the through holes are arranged so that primary roots of the wheat can grow in the partition cavity on one side along the through holes, the primary roots and secondary roots can be researched separately, the upper ends of the through holes are covered with soil by 1-2 cm, the rest parts of the pot edges are convenient to water, and runoff is prevented; the through hole 21 is an inclined hole forming an angle of 45 degrees with the horizontal plane, a conduit 3 wrapping the primary root and the underground stem is movably inserted in the through hole, the included angle between the conduit 3 and the partition plate is 45 degrees, the side wall of the conduit 3 is provided with a through opening so as to facilitate the operation of wrapping the primary root and the underground stem, the inclined hole is arranged to be close to the growth direction of the underground stem, the underground stem and the primary root can smoothly pass through the through hole conveniently, the influence on the through hole by the growth direction is not changed, the conduit is arranged in a mode that the side wall is provided with the through opening so as to smoothly pass the underground stem and the primary root into the conduit without damaging the underground stem and the primary root, the conduit is conveniently arranged to guide the underground stem to pass through the inclined hole, and the conduit needs to be removed after the underground stem passes through the inclined hole in the actual use process; the number of the partition plates is at least one, a plurality of partition plates can be arranged according to actual work requirements, the partition plate is one in the embodiment, the planting pot is square, the partition plate is located in the middle of the planting pot and is arranged in parallel with the side wall of the planting pot, the square is 20cm in width, the length and the height are both 30cm, and the size is selected to simulate the density of 270 ten thousand per hectare of basic seedlings of wheat crops to the maximum extent.
A test arrangement method of a wheat root division research device with different functional roots comprises the following steps: .
1. Selecting wheat seeds with plump seeds in the current year for later use, and culturing the seedlings in a seedling pond (which can be replaced by a pot plant);
2. watering the seedling raising pond properly before sowing (enough soil moisture at the bottom can be omitted), uniformly leveling the soil from the edge to the corner, removing surface soil, simply crushing and sieving for seedling raising, wherein the sieve mesh is 0.5 cm square, and the bottom layer is leveled again;
3. the method is characterized in that manual sowing is carried out in a seedling raising pool, the line spacing is 20cm, the plant spacing is 2cm, the sieved soil is used for covering, the soil covering depth is 5cm, and the soil covering depth is consistent, so that the seedlings can grow out of the stems in the field;
4. when the wheat seedlings grow to 1 leaf one-heart or two-leaf period, transplanting the seedlings, and selecting the seedlings which grow consistently and are provided with local mesostemma, wherein the local mesostemma length is about 1 cm; at the moment, root systems connected with the underground stems are all primary roots, the primary roots and the underground stems penetrate through the pores of the partition plates of the pot culture container, so that the primary roots are completely positioned in the space on one side of the container, the underground stems penetrate through the inclined pores of the partition plates, and the secondary roots grow in the space on the other side after the wheat is tillered; loading soil which is sieved before and is treated according to a test scheme into a pot according to weight, in the pot loading process, protecting primary roots as much as possible and enabling the primary roots to be tightly combined with the soil, when the pores of a partition board in the loaded soil are about 1cm, watering according to the calculated weight, and then covering the surface of the residual soil, wherein the soil surface just covers the pores of the partition board by 1-2 cm; and burying the transplanted pots in the field to complete the test arrangement, wherein the planting pots are made of plastic materials, and the planting density of the field test is 270 thousands of seedlings per hectare.

Claims (6)

1. The utility model provides a different function root system root-dividing research device of wheat which characterized in that: including planting the basin, be equipped with the division board in planting the basin, the space that the division board will plant in the basin divide into two planting chambeies, the position apart from top 4cm on the division board is equipped with one row of through-hole.
2. The wheat root rooting device with different functions, as claimed in claim 1, is characterized in that: the through hole is an inclined hole forming an angle of 45 degrees with the horizontal plane, a guide pipe is movably inserted in the through hole, the included angle between the guide pipe and the partition plate is 45 degrees, and a through opening is formed in the side wall of the guide pipe.
3. The wheat root rooting device with different functions, as claimed in claim 2, is characterized in that: the planting pot is square, and the partition plate is located in the middle of the planting pot and parallel to the side wall of the planting pot.
4. The device for root-dividing research on different functional root systems of wheat as claimed in claim 3, wherein: the square dimensions are 20cm wide and 30cm long and high.
5. A test arrangement method of a wheat root rooting research device with different functions as claimed in any one of claims 1 to 4, characterized by comprising the following steps:
(1) selecting wheat seeds and growing the wheat seeds in a seedling growing pond, wherein the row spacing is 20cm and the plant spacing is 2cm during sowing;
(2) transplanting the seedlings when the wheat seedlings grow to one heart stage or two leaves, selecting the seedlings which are robust, uniform and undamaged in growth, and have the root system complete and undamaged and the length of the underground stem of 1cm, placing the underground stem and the primary root in a guide pipe with an opening to penetrate through a through hole of a partition plate, enabling the underground stem of the wheat to be positioned in the through hole, placing the primary root on one side of the partition plate, filling soil and watering, burying a planting pot in the field to complete experimental arrangement, and after the wheat is tillered, growing the secondary root and positioning the partition plate on the other side, relative to the primary root, of the partition plate.
6. A test arrangement method of a wheat root-dividing research device with different functions as claimed in claim 5, characterized in that: the planting pot is made of plastic, and the planting density of a field test is 270 thousands of seedlings per hectare.
CN202210218810.9A 2022-02-28 2022-03-08 Root division research device for different functional root systems of wheat and test arrangement method Pending CN114431038A (en)

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CN2022101842887 2022-02-28
CN202210184288 2022-02-28

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Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002101772A (en) * 2000-09-28 2002-04-09 Tomohiko Sakamoto Plant protection material
TWM338548U (en) * 2007-10-30 2008-08-21 geng-hao Zhang Opening pipe used for grafting tomato
CN204616638U (en) * 2015-04-16 2015-09-09 中国林业科学研究院亚热带林业研究所 A kind of split-root experiment container
CN105165414A (en) * 2015-07-15 2015-12-23 广州市白云区农业科学试验中心 Seedling raising method for eggplants and tomatoes by grafting
CN206596432U (en) * 2017-02-14 2017-10-31 青岛农业大学 It is a kind of to distinguish wheat primary root, point root casing bit of secondary root
CN207083670U (en) * 2017-08-12 2018-03-13 广东飘之绿名木古树保护有限公司 A kind of ancient banian aerial root quick traction apparatus for descending
CN208029636U (en) * 2018-04-09 2018-11-02 菏泽学院 A kind of pretective sleeve pipe for transplanting peony plant
CN109122275A (en) * 2018-09-27 2019-01-04 西北农林科技大学 A kind of device dividing root culture suitable for aulophyte root system multicell
CN109258245A (en) * 2018-11-21 2019-01-25 广西壮族自治区农业科学院生物技术研究所 The guard method of passion fruit basal part of stem
CN209171112U (en) * 2018-11-09 2019-07-30 云南农业大学 It is a kind of for studying point root device of plant root nutrient solution
CN112997731A (en) * 2021-02-19 2021-06-22 农业农村部南京农业机械化研究所 Plant root absorption data collection device and method

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002101772A (en) * 2000-09-28 2002-04-09 Tomohiko Sakamoto Plant protection material
TWM338548U (en) * 2007-10-30 2008-08-21 geng-hao Zhang Opening pipe used for grafting tomato
CN204616638U (en) * 2015-04-16 2015-09-09 中国林业科学研究院亚热带林业研究所 A kind of split-root experiment container
CN105165414A (en) * 2015-07-15 2015-12-23 广州市白云区农业科学试验中心 Seedling raising method for eggplants and tomatoes by grafting
CN206596432U (en) * 2017-02-14 2017-10-31 青岛农业大学 It is a kind of to distinguish wheat primary root, point root casing bit of secondary root
CN207083670U (en) * 2017-08-12 2018-03-13 广东飘之绿名木古树保护有限公司 A kind of ancient banian aerial root quick traction apparatus for descending
CN208029636U (en) * 2018-04-09 2018-11-02 菏泽学院 A kind of pretective sleeve pipe for transplanting peony plant
CN109122275A (en) * 2018-09-27 2019-01-04 西北农林科技大学 A kind of device dividing root culture suitable for aulophyte root system multicell
CN209171112U (en) * 2018-11-09 2019-07-30 云南农业大学 It is a kind of for studying point root device of plant root nutrient solution
CN109258245A (en) * 2018-11-21 2019-01-25 广西壮族自治区农业科学院生物技术研究所 The guard method of passion fruit basal part of stem
CN112997731A (en) * 2021-02-19 2021-06-22 农业农村部南京农业机械化研究所 Plant root absorption data collection device and method

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
臧贺藏 等: "冬小麦初生根与次生根形态、生理性状差异分析", 《河南农业科学》 *
马元喜 等: "《小麦的根》", 31 October 1999, 中国农业出版社 *

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