CN109122275A - A kind of device dividing root culture suitable for aulophyte root system multicell - Google Patents
A kind of device dividing root culture suitable for aulophyte root system multicell Download PDFInfo
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- CN109122275A CN109122275A CN201811131699.XA CN201811131699A CN109122275A CN 109122275 A CN109122275 A CN 109122275A CN 201811131699 A CN201811131699 A CN 201811131699A CN 109122275 A CN109122275 A CN 109122275A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Abstract
The invention discloses a kind of devices for dividing root culture suitable for aulophyte root system multicell, are made of multicell main body and pedestal；Multicell main body is box-like structure, the bottom plate of box-like structure is equipped with several air holes, partition is equipped in box-like structure, partition is vertical with the bottom plate of box-like structure, and the inner space of box-like structure is separated into several points of root rooms by partition, partition top is equipped with expanding pyramidal structure from bottom to top, pyramidal structure, which is equipped with, divides root through-hole, and by dividing root through-hole to be connected to root room is divided, box-like structure base slab is equipped with card slot in pyramidal structure space, card slot is corresponding with partition position, and box-like structure base slab is equipped with support construction；Pedestal is the groove structure cooperated with support construction, and the baffle cooperated with card slot is equipped in groove structure, and the cell wall height of groove structure is less than the height of support construction.The present invention solves poor leakproofness in traditional root case apparatus, easy cross contamination, at high cost and complex structure and other problems.
Description
Technical field
The present invention relates to a kind of devices for dividing root culture suitable for aulophyte root system multicell.
Background technique
Root system of plant is important plant organ, can obtain moisture and nutriment needed for growth and development etc., related
The Basic of Biology research of root system of plant can illustrate the response mechanism that root system changes environmental factor.Edaphon is in plant
Growth, nutriment decomposition and inversion etc. all play between this important role, especially microorganism and host plant root system
The symbiosis of formation can be improved plant to the utilization efficiency of soil nutrient, in plant growth and development and improve the utilization of resources
It is played an important role in terms of efficiency.
However, previous root system research is all in a unification and uniform surrounding medium (such as flowerpot), but plant roots
Usually in growing environment complicated and changeable, especially most plants can form multiple symbiosis with multiple-microorganism for system
Relationship (such as leguminous plant can be with rhizobium and mycorrhizal fungi form three syntaxial system), will solve this problem in science,
The single training method of tradition just can not simulating plant complexity root growth environment.In addition, existing locellus root case is with close
Poor, the easy cross contamination of root air hole of envelope property, point root device occupied space is big, structure is complicated, technique is cumbersome and divides root operation tired
The disadvantages of difficult, causes such correlative study to face the problems such as error is big, and precision is low.Therefore, it is badly in need of wanting new culture apparatus with true
That protects aulophyte divides root research can be more accurate, more convenient.
Summary of the invention
In order to solve the deficiencies in the prior art, an object of the present invention is to provide a kind of more suitable for aulophyte root system
The device of root culture is divided in room, solves the shortage of dedicated point of root device of aulophyte and traditional root case dress in current root system research
Set poor middle leakproofness, easy cross contamination, at high cost and complex structure and other problems.
To achieve the goals above, the technical solution of the present invention is as follows:
A kind of device dividing root culture suitable for aulophyte root system multicell, is made of multicell main body and pedestal；
The multicell main body is the box-like structure formed by side wall and bottom plate, if the bottom plate of box-like structure is logical equipped with dry air
Hole, the box-like structure is interior to be equipped at least one partition, and the partition is vertical with the bottom plate of box-like structure, the bottom edge of the partition
With the contacts baseplate of box-like structure, the two sides of the partition are contacted with the side wall of box-like structure, and the partition is by box-like knot
The inner space of structure is separated into several points of root rooms, and the partition top is equipped with expanding pyramidal structure from bottom to top, the cone
Shape structure hollow, the pyramidal structure are equipped with several points of root through-holes, and the space in pyramidal structure passes through a point of root through-hole and one
A that root room is divided to be connected to, the lower surface of the box-like structure base slab is equipped with card slot, and the card slot is corresponding with partition position, the box-like
The lower surface of structure base slab is equipped with support construction；
The pedestal is the groove structure cooperated with support construction, and the baffle cooperated with card slot is equipped in groove structure, recessed
The cell wall height of slot structure is less than the height of support construction.
The present invention securely fixes plant by inverted pyramidal structure, to support the growth and development of its entire plant.It is logical
Excessive root through-hole can make root system of plant enter to difference and divide root room, to carry out a point root culture.It can will be entire by partition
Device is divided into multicell structure, can prevent the exchange that moisture, nutriment are carried out between multicell.Meanwhile the groove and card of pedestal
Slot can prevent it is different divide microorganism in root room, moisture by the exchange of air hole, thus increase locellus culture effect and
Accuracy.
The second object of the present invention is to provide a kind of above-mentioned apparatus in research bacterium to the induction of plant defense response and anti-
The application of the regulatory mechanism of inverse ability.
The third object of the present invention is to provide the induction and degeneration-resistant energy of a kind of Glomus versiforme to alfalfa defense reaction
The test method that the regulatory mechanism of amount influences, provides above-mentioned apparatus, alfalfa seedling is put into the pyramidal structure of device,
The soil of sterilizing is packed into each point of root room of device, the condition changed in the soil of each point of root room is cultivated, and the condition is
Moisture, Glomus versiforme and the two combination.
The invention has the benefit that
Root ring design is divided for taper in the device of the invention top, on the one hand, saves the design of volume, reserves more for plant
More growing spaces；On the other hand, since the vertical range of conical centre to the conical surface is most short, the exposed distance outside of root system is most
It is short, reduce the systematic error of experiment.In addition, the pedestal setting of bottom of device solves the ventable hole such as root moisture and microorganism
A possibility that mutually polluting.
Detailed description of the invention
The accompanying drawings constituting a part of this application is used to provide further understanding of the present application, and the application's shows
Meaning property embodiment and its explanation are not constituted an undue limitation on the present application for explaining the application.
Fig. 1 is the structural schematic diagram of apparatus of the present invention；
Fig. 2 is the bottom substance schematic diagram of multicell main body；
Fig. 3 is the structural schematic diagram of pedestal；
Fig. 4 is test schematic diagram of the AMF to the induction of alfalfa defense reaction and the regulatory mechanism research of anti-adversity ability；
Wherein, 1. multicell main body, 2. pedestals, 3. partitions, 4. pyramidal structures, 5. points of root through-holes, 6. support constructions, 7. gears
Plate, 8. air holes, 9. card slots.
Specific embodiment
It is noted that described further below be all exemplary, it is intended to provide further instruction to the application.Unless another
It indicates, all technical and scientific terms used herein has usual with the application person of an ordinary skill in the technical field
The identical meanings of understanding.
It should be noted that term used herein above is merely to describe specific embodiment, and be not intended to restricted root
According to the illustrative embodiments of the application.As used herein, unless the context clearly indicates otherwise, otherwise singular
Also it is intended to include plural form, additionally, it should be understood that, when in the present specification using term "comprising" and/or " packet
Include " when, indicate existing characteristics, step, operation, device, component and/or their combination.
Support construction described herein is that can support multicell main body, after multicell main body and base engagement, can be made
There are gaps between multicell main body and the groove structure of pedestal, and groove structure is enable to pass through the atmosphere outside the gap and device
Structure of connection, such as supporting leg, support plate, support frame etc..
As background technique is introduced, exist in the prior art poor leakproofness in traditional root case apparatus, easy cross contamination,
Deficiency at high cost and that structure is complicated waits is suitable for symbiosis present applicant proposes one kind and plants to solve technical problem as above
Object root system multicell divides the device of root culture.
A kind of exemplary embodiment of the application provides a kind of dress for dividing root culture suitable for aulophyte root system multicell
It sets, is made of multicell main body and pedestal；
The multicell main body is the box-like structure formed by side wall and bottom plate, if the bottom plate of box-like structure is logical equipped with dry air
Hole, the box-like structure is interior to be equipped at least one partition, and the partition is vertical with the bottom plate of box-like structure, the bottom edge of the partition
With the contacts baseplate of box-like structure, the two sides of the partition are contacted with the side wall of box-like structure, and the partition is by box-like knot
The inner space of structure is separated into several points of root rooms, and the partition top is equipped with expanding pyramidal structure from bottom to top, the cone
Shape structure hollow, the pyramidal structure are equipped with several points of root through-holes, and the space in pyramidal structure passes through a point of root through-hole and one
A that root room is divided to be connected to, the lower surface of the box-like structure base slab is equipped with card slot, and the card slot is corresponding with partition position, the box-like
The lower surface of structure base slab is equipped with support construction；
The pedestal is the groove structure cooperated with support construction, and the baffle cooperated with card slot is equipped in groove structure, recessed
The cell wall height of slot structure is less than the height of support construction.
The application securely fixes plant by inverted pyramidal structure, to support the growth and development of its entire plant.It is logical
Excessive root through-hole can make root system of plant enter to difference and divide root room, to carry out a point root culture.It can will be entire by partition
Device is divided into multicell structure, can prevent the exchange that moisture, nutriment are carried out between multicell.Meanwhile air is root system of plant
Necessary to growth, oxygen deficiency will lead to the breathings such as root system of plant and microorganism and be obstructed, and can also influence nutriment in soil
Therefore mutual conversion is provided with several air holes for ventilating in the bottom plate of multicell main body；However, the moisture of multicell
Or the ventable aperture such as microorganism mutually exchanges, this will affect the effect and accuracy of locellus culture, in order to solve this
Problem, the groove of the application pedestal and card slot can prevent it is different divide microorganism in root room, moisture by the exchange of air hole,
To increase the effect and accuracy of locellus culture.
Since plant lateral roots are mainly distributed near the ground, in order to save space, reduce cost, one kind of the embodiment or
In various embodiments, box-like structure is gradually decreased from bottom plate to the radial section of opposite side.
In one or more embodiments of the embodiment, top side length, bottom side length, the height of device of box-like structure
Ratio be 8:6~7:9~11.The shape not only reduced the production cost, but also did not influenced the distribution and growth of root system.The box-like knot
Structure radial section is square or circle；If being square, top side length, bottom side length be respectively top square side length,
The side length of bottom square；If circle, top side length, bottom side length be respectively the diameter of top circular, bottom circular it is straight
Diameter.
In one or more embodiments of the embodiment, the diameter of the circular open of pyramidal structure and the top of box-like structure
The ratio of portion's side length is 1~2:8.
In order to simplify the structure of device, in one or more embodiments of the embodiment, one is equipped in box-like structure
Partition.
In one or more embodiments of the embodiment, the support construction is two support plates, two support plate bottoms
Plate, which contacts, to be formed the contact of straight line, partition with bottom plate to form straight line parallel.It can guarantee the air circulation of bottom of device.
In one or more embodiments of the embodiment, prepared using the method for 3D printing.
The culture apparatus of the production of previous plastic material in use, intolerant to exposing to the sun and rain, less acid-alkali-corrosive-resisting,
Therefore its service life is shorter, and in order to make device that there is high temperature resistant, wear-resistant, corrosion-resistant, the features such as quality is lighter, the embodiment
One or more embodiments in, the material of device is polyvinyl resin material.This material lowers costs of manufacture, improve dress
The service efficiency etc. set.
The another embodiment of the application provides a kind of above-mentioned apparatus and lures in research bacterium plant defense response
It leads and the application of the regulatory mechanism of anti-adversity ability.
Moisture or microorganism are by dividing root through-hole to leak in order to prevent, in one or more embodiments of the embodiment,
By root system of plant by dividing root through-hole to enter each point of root room after, divide root through-hole using nylon wire or sponge blocking.
The application the third embodiment there is provided a kind of Glomus versiformes to the induction of alfalfa defense reaction and
The test method that the regulatory mechanism of degeneration-resistant energy influences, provides above-mentioned apparatus, alfalfa seedling is put into the taper of device
In structure, the soil of sterilizing is packed into each point of root room of device, the condition changed in the soil of each point of root room is cultivated, described
Condition is moisture, Glomus versiforme and the two combination.
In order to enable those skilled in the art can clearly understand the technical solution of the application, below with reference to tool
The technical solution of the application is described in detail in the embodiment of body.
Embodiment
A kind of device dividing root culture suitable for aulophyte root system multicell, as shown in Figures 1 to 3, by multicell main body 1 and bottom
Seat 2 forms.
Multicell main body 1 is the box-like structure formed by side wall and bottom plate.The bottom plate of box-like structure is equipped with several air holes
8.1 partition 3 is equipped in box-like structure.Partition 3 is vertical with the bottom plate of box-like structure.The bottom edge of partition 3 and the bottom of box-like structure
Plate contact.The two sides of partition 3 are contacted with the side wall of box-like structure.The inner space of box-like structure is separated into 2 by partition 3
Divide root room.3 top of partition is equipped with expanding pyramidal structure 4 from bottom to top, and pyramidal structure 4 is hollow.Pyramidal structure 4 is equipped with 2
Divide root through-hole 5, the space in pyramidal structure 4 is connected to by a point of root through-hole 5 with a point of root room.Under box-like structure base slab
Surface is equipped with card slot 9, and card slot 9 is corresponding with 3 position of partition.The lower surface of box-like structure base slab is equipped with support construction 6.
Pedestal is the groove structure cooperated with support construction 6, is equipped with the baffle 7 cooperated with card slot 9, groove in groove structure
The cell wall height of structure is less than the height of support construction 6.
Box-like structure is gradually decreased from bottom plate to the radial section of opposite side.
Box-like structure radial section is square.The top square side length of box-like structure is 8cm, bottom square side length
Height for 6.5cm, device is 10cm.The diameter of the circular open of pyramidal structure 4 is 1.5cm.
Support construction 6 is two support plates, and two support plate contacts baseplates form the contact shape of straight line, partition 3 with bottom plate
It is in line parallel.
The device is made using polyvinyl resin as raw material by 3D printing technique.
AMF is carried out using the device to study the induction of alfalfa defense reaction and the regulatory mechanism of anti-adversity ability.
Test material and method
It is test plant that Medicago sativacv (Medicago sativa L.cv.Algonquin) is selected in test.Kind
Son is purchased from Beijing right way Eco Science Technology Co., Ltd.It is Glomus versiforme (Glomus constrictum) for examination AMF, by Beijing
City's agricultural and forest science institute plant nutrient and the Study on Resources institute " arbuscular mycorrhizal fungi germplasm resource bank " (BGC) are provided, mycorhiza inoculum
Mixture by expanding numerous plant root segment, mycelia, spore and sandy soil forms.Rhizobium (Sinorhizobium meliloti
2011) it is taught from Chinese Academy of Sciences Shanghai life science institute Wang Yanzhang.Test preceding pasteurizing solution to greenhouse, seedbed and
Flowerpot used carries out disinfection processing.Soil used is derived from field arable layer, and main nutrient composition is as follows: C (13.5g/kg), complete
N(1.26g/kg)、NO3 -(7.52mg/kg)、NH4 +(45.67mg/kg)、P(0.8g/kg)、K(8.29mg/kg)、Ca
(92.85mg/kg),Na(138.39mg/kg).Before soil fills basin, preparatory sieving and the high-temperature sterilization 48h in 170 DEG C of baking oven.
Full, uniform and healthy alfalfa seed is selected, the hypochlorite disinfectant 2min for being 0.1% with concentration, distilled water cleaning
It is placed in culture dish and germinates after clean.Germinate 3~4d in 4 DEG C of dark situation.It chooses and grows uniform seedling replanting to being equipped with
Water planting 4 weeks (long 20cm, wide 15cm, high 7cm) in the culture vessel of 2L nutrient solution.It selects and grows uniform plant seedlings, by palpus
Root is divided into two parts, and foundation divides root cultivating system.Seedling cultivation is in experimental rig as shown in the figure.Flowerpot after transplanting is put
At 25/18 DEG C of temperature (day/night), relative humidity 40%, the photoperiod is in the growing environment of 16/8h (light dark).
Experimental design
The signal between system response mechanism and homobium after receiving drought stress signal for clear mycorrhizas homobium turns
Approach is led, in dividing root cultivating system, is provided with Local treatment, i.e. side carries out drought stress processing, and the other side remains normal
Moisture condition；Side carries out inoculation processing simultaneously, and the other side is not inoculated with processing.Thus can be formed in total two it is non-uniform
Culture systems: (1) drought stress processing+maintain normal moisture condition in non-mycorhiza room in mycorhiza room is carried out；(2) in non-bacterium
Drought stress processing+normal moisture condition is maintained in mycorhiza room is carried out in root room.Osmotic treatment is imposed rather than bacterium in mycorhiza room
Root room maintains the dividing in root culture systems of normal moisture condition, it is contemplated that the influence of inoculation and the two factors of moisture joined 3
1. how a control treatment, 2. and 3. (Fig. 4), carrys out clear AM homobium when coping with drought stress to host plant entirety with this
Regulated and controled, to improve survival ability of the host plant in heterogeneous environment.Therefore, (the following letter of root culture systems is divided at this
Referred to as system (1)) in, totally 4 processing-(1), 1., 2. and 3., each processing is repeated 5 times, total 20 basins.1 plant of plant of every basin,
Test the arrangement of basin alms bowl completely random.Be arranged non-mycorhiza room impose Osmotic treatment and mycorhiza room maintain normal moisture condition divide root to train
Feeding system is to disclose the feedback mechanism that AM homobium copes with external stress signal.Be related in this system one inoculation because
Element, therefore 1 control treatment is added 1..Divide in root culture systems (hereinafter referred to as system (2)) at this, totally 2 processing-(2)
1. each processing is repeated 5 times, amount to 10 basins.1 plant of plant of every basin, test basin alms bowl completely random arrangement.
Trial
During test, soil moisture content is controlled with weight method, and weighing is primary daily, supplies the water of loss.It is testing
Early period is handled, maintains the soil moisture content of each basin to 12% (based on soil dry weight, about the 70% of water-retaining quantity among field of soil).Root
According to the daily evaporated water of every basin, each locellus respectively adds the half of daily evaporated water.After plant growth 25 days, by Fig. 4 into
Row moisture solution.For two locellus there are two the processing of the gradation of moisture, the locellus of Osmotic treatment adds the 1/4 of daily evaporated water,
The locellus of normal moisture solution adds 3/4 (soil moisture content is about 12%) of daily evaporated water.For with considerable moisture ladder
Two locellus of degree, each locellus respectively add the half of daily evaporated water.Moisture solution carries out harvest and correlation after continuing 10 days
Index determining.
The foregoing is merely preferred embodiment of the present application, are not intended to limit this application, for the skill of this field
For art personnel, various changes and changes are possible in this application.Within the spirit and principles of this application, made any to repair
Change, equivalent replacement, improvement etc., should be included within the scope of protection of this application.
Claims (10)
1. a kind of device for dividing root culture suitable for aulophyte root system multicell, characterized in that be made of multicell main body and pedestal；
The multicell main body is the box-like structure formed by side wall and bottom plate, and the bottom plate of box-like structure is equipped with several air holes,
At least one partition is equipped in the box-like structure, the partition is vertical with the bottom plate of box-like structure, the bottom edge of the partition and case
The contacts baseplate of shape structure, the two sides of the partition are contacted with the side wall of box-like structure, and the partition is by box-like structure
Inner space is separated into several points of root rooms, and the partition top is equipped with expanding pyramidal structure from bottom to top, the taper knot
Structure is hollow, and the pyramidal structure is equipped with several points of root through-holes, and the space in pyramidal structure passes through a point of root through-hole and one point
The lower surface of the connection of root room, the box-like structure base slab is equipped with card slot, and the card slot is corresponding with partition position, the box-like structure
The lower surface of bottom plate is equipped with support construction；
The pedestal is the groove structure cooperated with support construction, is equipped with the baffle cooperated with card slot, groove knot in groove structure
The cell wall height of structure is less than the height of support construction.
2. device as described in claim 1, characterized in that box-like structure is gradually decreased from bottom plate to the radial section of opposite side.
3. device as claimed in claim 2, characterized in that the top side length of box-like structure, bottom side length, the height of device
Than for 8:6~7:9~11.
4. device as described in claim 1, characterized in that the diameter of the circular open of pyramidal structure and the top of box-like structure
The ratio of side length is 1~2:8.
5. device as described in claim 1, characterized in that be equipped with a partition in box-like structure.
6. device as described in claim 1, characterized in that the support construction is two support plates, two support plate bottom plates
Contact forms the contact of straight line, partition with bottom plate, and to form straight line parallel.
7. device as described in claim 1, characterized in that prepared using the method for 3D printing；
Or, the material of device is polyvinyl resin material.
8. induction and anti-adversity ability of a kind of any device of claim 1~7 in research bacterium to plant defense response
Regulatory mechanism application.
9. application as claimed in claim 8, characterized in that by root system of plant by dividing root through-hole to enter each point of root room after, adopt
Divide root through-hole with nylon wire or sponge blocking.
10. the test side that a kind of Glomus versiforme influences the regulatory mechanism of the induction of alfalfa defense reaction and degeneration-resistant energy
Method, characterized in that provide claim 1~7 any device, alfalfa seedling is put into the pyramidal structure of device
In, the soil of sterilizing is packed into each point of root room of device, the condition changed in the soil of each point of root room is cultivated, the condition
For the combination of moisture, Glomus versiforme and the two.
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| CN110915478A (en) * | 2019-12-20 | 2020-03-27 | 南京兴华建筑设计研究院股份有限公司 | Assembled 3D prints planting system |
| CN114431038A (en) * | 2022-02-28 | 2022-05-06 | 河南农业大学 | Root division research device for different functional root systems of wheat and test arrangement method |
| CN115316157A (en) * | 2022-09-26 | 2022-11-11 | 贵州省中国科学院天然产物化学重点实验室(贵州医科大学天然产物化学重点实验室) | Mycorrhiza seedling raising device capable of efficiently infecting honeysuckle and use method thereof |
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