CN110487585A - Rhizosphere sampler and application method for the research of continuous cropping soil distress mechanism - Google Patents
Rhizosphere sampler and application method for the research of continuous cropping soil distress mechanism Download PDFInfo
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- CN110487585A CN110487585A CN201910891068.6A CN201910891068A CN110487585A CN 110487585 A CN110487585 A CN 110487585A CN 201910891068 A CN201910891068 A CN 201910891068A CN 110487585 A CN110487585 A CN 110487585A
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- rhizosphere
- root
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- access panel
- plate
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- 239000002689 soil Substances 0.000 title claims abstract description 62
- 238000011160 research Methods 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims abstract description 25
- 230000007246 mechanism Effects 0.000 title claims abstract description 23
- 230000009429 distress Effects 0.000 title claims abstract description 22
- 238000009335 monocropping Methods 0.000 title claims abstract description 22
- 238000005070 sampling Methods 0.000 claims abstract description 31
- 238000005192 partition Methods 0.000 claims abstract description 29
- 238000005520 cutting process Methods 0.000 claims abstract description 23
- 230000002786 root growth Effects 0.000 claims description 8
- 244000052616 bacterial pathogen Species 0.000 claims description 6
- 201000010099 disease Diseases 0.000 claims description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 6
- 238000009434 installation Methods 0.000 claims description 6
- 239000000706 filtrate Substances 0.000 claims description 4
- 238000004891 communication Methods 0.000 claims description 3
- 244000005700 microbiome Species 0.000 claims description 3
- 238000002054 transplantation Methods 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 238000001727 in vivo Methods 0.000 claims description 2
- 230000001360 synchronised effect Effects 0.000 abstract description 3
- 238000005527 soil sampling Methods 0.000 abstract description 2
- 238000010586 diagram Methods 0.000 description 8
- 230000008569 process Effects 0.000 description 7
- 230000012010 growth Effects 0.000 description 3
- 239000010410 layer Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 239000011229 interlayer Substances 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000036285 pathological change Effects 0.000 description 2
- 231100000915 pathological change Toxicity 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 206010011409 Cross infection Diseases 0.000 description 1
- VVQNEPGJFQJSBK-UHFFFAOYSA-N Methyl methacrylate Chemical compound COC(=O)C(C)=C VVQNEPGJFQJSBK-UHFFFAOYSA-N 0.000 description 1
- 206010029803 Nosocomial infection Diseases 0.000 description 1
- 229920005372 Plexiglas® Polymers 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 239000002360 explosive Substances 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000001863 plant nutrition Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01D—HARVESTING; MOWING
- A01D91/00—Methods for harvesting agricultural products
- A01D91/02—Products growing in the soil
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/028—Multi-compartmented pots
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Abstract
The present invention discloses a kind of rhizosphere sampler and application method for the research of continuous cropping soil distress mechanism, it is related to rhizosphere soil sampling technical field, the side of cabinet is provided with installing port, rhizosphere access panel is removably installed on installing port, and root room partition is set in cabinet and cabinet is divided into first room and second room；Root room partition top is provided with for installing the mounting groove every root plate, mounting groove upper surface is provided with multiple first grooves, root plate lower surface is provided with multiple second grooves, corresponding first groove and the second groove form one and lead root hole, respectively lead the lower section that root plate is removably installed in first groove, and lead root plate and be installed on root room partition close to the side of rhizosphere access panel, rhizosphere sampler includes multiple cutting blades disposed in parallel.The device and application method precise positioning morbidity rhizosphere area and can realize the synchronized sampling research of root system, Rhizosphere Soil, Non-rhizosphere soil.
Description
Technical field
The present invention relates to rhizosphere soil sampling technical fields, study more particularly to one kind for continuous cropping soil distress mechanism
Rhizosphere sampler and application method.
Background technique
Rhizosphere is the important area that crop root and soil carry out nutrient and energy exchange, is that edaphon is most active
Place is the specific region for being influenced by crop root growth, while influencing the absorption and transhipment of crop nutrition content again.Rhizosphere at present
Therefore, rhizosphere micro-region is always that domestic and international agrology, Plant Nutrition, agronomy etc. are multidisciplinary for micro- domain and the research of phase edaphon
One of focus of research.
The sampling method of conventional rhizosphere soil is taking human as based on shake partition method, but there are sampling quantities inhomogenous, precision
Low problem；Research is closely affixed using the more interlayer root casees in three Room or by interlayer net again, is carried out the sampling of millimeter rank, but is filled
Native tight degree degree has an impact to rhizosphere spacing distance, while being sampled as the non-rhizosphere aggregate sample of rhizosphere, non-absolute sense Rhizosphere Soil,
The site of action of root system can not accurately be studied；In addition there are researchs to use freezing method, this right method slice thickness is not easy to control,
And slice can break ring root system of plant, can not achieve rhizosphere root system and sample simultaneously with Rhizosphere Soil, Non-rhizosphere soil, while quick-frozen process meeting
Change the nutrient situation and microbial activity in the soil body.Special precise positioning morbidity is studied for soil blockage mechanism of being punished for being related at present
The root case apparatus in rhizosphere region has not been reported.
Summary of the invention
In order to solve the above technical problems, the present invention provides a kind of rhizosphere sampling dress for the research of continuous cropping soil distress mechanism
It sets and application method, precise positioning morbidity rhizosphere area and the synchronized sampling research for realizing root system, Rhizosphere Soil, Non-rhizosphere soil.
To achieve the above object, the present invention provides following schemes:
The present invention provides a kind of rhizosphere sampler for the research of continuous cropping soil distress mechanism, the case including upper opening
Body, rhizosphere access panel, root room partition, every root plate, sampling plate, rhizosphere sampler and it is multiple lead root plate, the side of the cabinet is set
It is equipped with installing port, the rhizosphere access panel is removably installed on the installing port, and described room partition is set to the case
In vivo and the cabinet is divided into first room and second room, and described room partition is parallel with the rhizosphere access panel sets
It sets, far from the rhizosphere access panel, first room is used for placing normal soil sample, second room for first room
It carries disease germs soil sample in placement, the lower end of first room and second room is each provided with a filtrate pipe and is in communication with the outside；
Described room partition top is provided with for installing the mounting groove every root plate, and the mounting groove upper surface is provided with multiple the
One groove, described that root plate lower surface is provided with multiple second grooves, first groove and second groove correspond
And structure matches, corresponding first groove and second groove form one and lead root hole, it is each it is described lead root plate can
It is releasably installed on the lower section of first groove, and the root plate of leading is installed on described room partition close to the rhizosphere
The side of access panel, the rhizosphere sampler include multiple cutting blades disposed in parallel.
Preferably, there are two being symmetrical arranged on two side plate inner walls of the cabinet for installing the rhizosphere access panel
Board slot is sampled, the sampling board slot is disposed in proximity to one end of the rhizosphere access panel, and the sampling plate is installed on the sampling
It is arranged in parallel after in board slot with the rhizosphere access panel.
Preferably, the two sides every root board ends top are each provided with a limit plate.
Preferably, the two sides of described room partition upper end are each provided with a fixed knob, and the fixed knob is for tight
Gu described every root plate.
Preferably, described to lead root hole by one end far from the rhizosphere access panel to one end close to the rhizosphere access panel
Tilt down setting.
Preferably, described room partition is provided with multiple card slots close to the side of the rhizosphere access panel, the card slot with
First groove corresponds, and a card slot is located at the lower section of first groove, and a card slot is used
In installation one described in lead root plate.
Preferably, the cross section for leading root plate is V-shaped.
Preferably, the rhizosphere sampler further include operating stick, laser shot-light, bearing roller, bearing fixing rod, battery,
Away from gasket, the operating stick inner hollow forms battery compartment, described for battery cover, power knob, two hold-doun nuts and multiple controls
Battery compartment is set to above the battery compartment for placing the battery, the battery cover, and the laser shot-light is set to described
The lower part of operating stick downside, the power knob are set to the top of the operating stick upper side, and the battery is used for institute
The power supply of laser shot-light is stated, the power knob is used to control the opening and closing of the battery, and the bearing roller is fixed on the operation
Bar lower end, the bearing fixing rod fixing sleeve are set to inside the bearing roller, and the control is away from gasket and the cutting blade set
Outside the bearing fixing rod, the both ends of the bearing fixing rod are separately installed with the hold-doun nut, adjacent
The control is provided between two cutting blades away from gasket.
The present invention also provides a kind of application methods of rhizosphere sampler for the research of continuous cropping soil distress mechanism, including
Following steps:
Step 1 fills normal soil sample in first room Xiang Suoshu, and filling is carried disease germs soil sample in second room Xiang Suoshu, bankets
Root plate is led after reaching a certain height, described in installation, and water planting transplantation of seedlings to first room passes through the root of Rooted Cuttings described
Lead root hole, into it is described lead root plate after, lead root plate described in closure is fixed, continue earthing；
Step 2 checks root growth and incidence by described room access panel；
Step 3 cuts soil layer in the side close to described room access panel by sampling plate；
The cabinet is laid flat so that described room access panel is located above by step 4, and described room access panel of opening is adopted
It is cut with the rhizosphere sampler along root system；Root system is taken out, root table microorganism and root system plant sample are extracted；Using thin blade
Along separation soil extract sample.
The present invention achieves following technical effect compared with the existing technology:
Provided by the present invention for the rhizosphere sampler and application method of the research of continuous cropping soil distress mechanism, using isolation
Root room is designed, and root growth is normal in first room, and root system is fallen ill in second room, it is ensured that root system of plant orientation morbidity.Pass through
Setting, which leads root hole and leads root plate, realizes root system guidance, so that the root system patch root room access panel growth in second room, can observe
Crop root growth germ infection processs and prevention and treatment process.By observation rhizosphere morbidity dynamic process, determine that root system morbidity is crucial
Region, open root room access panel, using rhizosphere sampler realize rhizosphere region precisely orient sampling, while obtain site of pathological change and
Its Rhizosphere Soil, Non-rhizosphere soil.Root hole design is led using separate type, avoids root system disturbance or mechanical injuries.
Detailed description of the invention
It in order to more clearly explain the embodiment of the invention or the technical proposal in the existing technology, below will be to institute in embodiment
Attached drawing to be used is needed to be briefly described, it should be apparent that, the accompanying drawings in the following description is only some implementations of the invention
Example, for those of ordinary skill in the art, without creative efforts, can also obtain according to these attached drawings
Obtain other attached drawings.
Fig. 1 is the structural schematic diagram for the rhizosphere sampler studied provided by the present invention for continuous cropping soil distress mechanism；
Fig. 2 is that the rhizosphere sampler studied provided by the present invention for continuous cropping soil distress mechanism is not installed when root plate
Structural schematic diagram；
Fig. 3 is the partial enlarged view of second room in the present invention；
Fig. 4 is the structural schematic diagram of first chamber component in the present invention；
Fig. 5 is the structural schematic diagram of second chamber component in the present invention；
Fig. 6 is root room partition and the structural schematic diagram every root plate in the present invention；
Fig. 7 is the structural schematic diagram that root plate is led in the present invention；
Fig. 8 is the structural schematic diagram of rhizosphere sampler in the present invention；
Fig. 9 is the explosive view of rhizosphere sampler in the present invention；
Figure 10 is schematic diagram when installing sampling plate in the present invention；
Figure 11 is overall schematic when rhizosphere sampler is sampled in the present invention；
Figure 12 is partial enlarged view when rhizosphere sampler is sampled in the present invention.
Description of symbols: 1, first chamber component；2, second chamber component；3, root room partition；4, every root plate；5, it limits
Plate；6, rhizosphere access panel；7, board slot is sampled；8, filtrate pipe；9, root plate is led；10, fixed knob；11, the first groove；12, second
Groove；13, card slot；14, sampling plate；15, operating stick；16, power knob；17, battery cover；18, laser shot-light；19, bearing rolls
Wheel；20, bearing fixing rod；21, cutting blade；22, control is away from gasket；23, hold-doun nut；24, light beam is guided.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
The object of the present invention is to provide a kind of rhizosphere sampler for the research of continuous cropping soil distress mechanism and application sides
Method, precise positioning morbidity rhizosphere area and the synchronized sampling research for realizing root system, Rhizosphere Soil, Non-rhizosphere soil.
In order to make the foregoing objectives, features and advantages of the present invention clearer and more comprehensible, with reference to the accompanying drawing and specific real
Applying mode, the present invention is described in further detail.
As shown in figs 1-9, the present embodiment provides a kind of rhizosphere sampler for the research of continuous cropping soil distress mechanism, packets
It includes the cabinet of upper opening, rhizosphere access panel 6, root room partition 3, every root plate 4, sampling plate 14, rhizosphere sampler and multiple lead root
Plate 9, the side of cabinet are provided with installing port, and rhizosphere access panel 6 is removably installed on installing port, and root room partition 3 is set to
First room and second room are divided into cabinet and by cabinet, and root room partition 3 is arranged in parallel with rhizosphere access panel 6, first
Root room is far from rhizosphere access panel 6, and first room is for placing normal soil sample, and second room is for placing soil sample of carrying disease germs, and first
The lower end of room and second room is each provided with a filtrate pipe 8 and is in communication with the outside；3 top of root room partition be provided with for install every
The mounting groove of root plate 4, mounting groove upper surface are provided with multiple first grooves 11, and it is recessed that 4 lower surface of root plate is provided with multiple second
Slot 12, the first groove 11 is corresponded with the second groove 12 and structure matches, corresponding first groove 11 and the second groove
12, which form one, leads root hole, respectively leads the lower section that root plate 9 is removably installed in first groove 11, and lead root plate 9 and be installed on
For root room partition 3 close to the side of rhizosphere access panel 6, rhizosphere sampler includes multiple cutting blades disposed in parallel 21.
It designing in the present embodiment using isolation root room, root growth is normal in first room, and root system is fallen ill in second room,
Ensure root system of plant orientation morbidity.Root hole is led by setting and leads root plate 9 and realizes root system guidance, so that the root system in second room
The growth of root room access panel is pasted, crop root growth germ infection processs and prevention and treatment process can be observed.Pass through observation rhizosphere morbidity
Dynamic process determines that root system is fallen ill key area, is opened root room access panel, is realized that soil is punished for being related to obstacle machine using rhizosphere sampler
Reason research morbidity rhizosphere region precisely orients sampling, and the rhizosphere sampler with multiple cutting blades 21 makes it possible to obtain simultaneously
Site of pathological change and its Rhizosphere Soil, Non-rhizosphere soil.Root hole design is led using separate type, avoids root system disturbance or mechanical injuries.It adopts
With the sampling plate 14 of cutting type, nearly rhizosphere growth soil layer face can be obtained, convenient for taking Rhizosphere sampling.
In this specific embodiment, root room access panel is transparent plexiglass plate, and the two sides of root room access panel pass through screw
On two side plates being installed at the installing port of cabinet with nut.
In this specific embodiment, cabinet includes first chamber component 1 and second chamber component 2, first 1 He of chamber component
Second 2 both ends of chamber component docks and root room partition 3 is clamped in centre, then uses screw and nut by two sides of three
While being fixed.
It is symmetrical on two side plate inner walls of the cabinet for installing rhizosphere access panel 6 in order to be oriented to sampling plate 14
Setting is disposed in proximity to one end of rhizosphere access panel 6 there are two board slot 7, sampling board slot 7 is sampled, and sampling plate 14 is installed on sampling plate
It is arranged in parallel after in slot 7 with rhizosphere access panel 6.In this specific embodiment, sample between board slot 7 and rhizosphere access panel 6
Distance is 0.4cm, and sampling plate 14 is the stainless steel plate of thickness 1mm.
As shown in Fig. 3,6, in order to limit moving forward and backward every root plate 4, one is each provided with every the two sides on 4 both ends top of root plate
A limit plate 5.In order to limit the upper and lower displacement every root plate 4, the two sides of 3 upper end of root room partition are each provided with a fixed knob
10, fixed knob 10 is for fastening every root plate 4.In use, will after root plate 4 is placed in mounting groove, limit plate 5 to its into
Row limits in the front-back direction, rotation fixed knob 10 to the limit for carrying out up and down direction to it above root plate 4, be achieved in every
The leakage of generation soil is dynamic between first room and second room and root system is worn in preventing on root room partition 3 for 4 firm installation of root plate
Thoroughly, it influences to observe and sample.
Specifically, it leads root hole and is tilted down and set to one end close to rhizosphere access panel 6 by one end far from rhizosphere access panel 6
It sets, forms height fall, avoid generating the cross-infection of soil germ between first room and second room, influence test result.
Specifically, root room partition 3 is provided with multiple card slots 13 close to the side of rhizosphere access panel 6, card slot 13 and first recessed
Slot 11 corresponds, and a card slot 13 is located at the lower section of first groove 11, and a card slot 13 leads root for installing one
Plate 9.In this specific embodiment, the cross section for leading root plate 9 is V-shaped.
As shown in Figure 8,9, rhizosphere sampler further includes operating stick 15, laser shot-light 18, bearing roller 19, bearing fixing rod
20, battery, battery cover 17,16, two hold-doun nuts 23 of power knob and multiple controls are away from gasket 22,15 inner hollow shape of operating stick
At battery compartment, battery compartment is used for placing battery, and battery cover 17 is set to above battery compartment, and laser shot-light 18 is set to operating stick 15
The lower part of downside, power knob 16 are set to the top of 15 upper side of operating stick, and battery is used to power to laser shot-light 18, electricity
Source button 16 is used to control the opening and closing of battery, and bearing roller 19 is fixed on 15 lower end of operating stick, and the fixation of bearing fixing rod 20 is arranged
Inside bearing roller 19, control is sheathed on outside bearing fixing rod 20 away from gasket 22 and cutting blade 21, bearing fixing rod 20
Both ends are separately installed with a hold-doun nut 23, and a control is provided between two adjacent cutting blades 21 away from gasket 22, is led to
The control of replacement different-thickness is crossed away from adjustable adjacent the distance between two cutting blades 21 of gasket 22, and then can basis
Actual demand adjusts cutting distance, promotes the scope of application.
In use, opening laser shot-light 18 by power knob 16, laser shot-light 18 issues guidance 24 pairs of cutting sides of light beam
To being guided, so that cutting sampling is more accurate.By using the rhizosphere sampler designed in the present embodiment, realize away from
Precisely sampled from root system different distance soil, apart from root system distance determine, compared with liquid nitrogen frozen cutting method, it is with clearly defined objective and
Plant sample is not destroyed.
In this specific embodiment, the two sides of bearing roller 19 are respectively equipped with 3 cutting blades 21.
The present embodiment also provides a kind of application method of rhizosphere sampler for the research of continuous cropping soil distress mechanism, packet
Include following steps:
Step 1 fills normal soil sample into first room, and soil sample of carrying disease germs is filled into second room, bankets and reaches certain
After height, root plate 9 is led in installation, and by water planting transplantation of seedlings to first room, Rooted Cuttings growing way is consistent and without germ, the root of Rooted Cuttings
Across root hole is led, into after leading root plate 9, closure is fixed to lead root plate 9, continues earthing；
Step 2 checks root growth and incidence by root room access panel；
Step 3 cuts the soil layer of 0.4cm in the side close to root room access panel by sampling plate 14 as shown in Figure 10；
Cabinet is laid flat so that root room access panel is located above as shown in Figure 11,12, opens root room access panel by step 4,
It is cut using rhizosphere sampler along root system；Root system is taken out, root table microorganism and root system plant sample are extracted；Using thin blade edge
Separation soil extract sample.
Apply that a specific example illustrates the principle and implementation of the invention in this specification, above embodiments
Explanation be merely used to help understand method and its core concept of the invention；At the same time, for those skilled in the art,
According to the thought of the present invention, there will be changes in the specific implementation manner and application range.In conclusion in this specification
Appearance should not be construed as limiting the invention.
Claims (9)
1. a kind of rhizosphere sampler for the research of continuous cropping soil distress mechanism, which is characterized in that the case including upper opening
Body, rhizosphere access panel, root room partition, every root plate, sampling plate, rhizosphere sampler and it is multiple lead root plate, the side of the cabinet is set
It is equipped with installing port, the rhizosphere access panel is removably installed on the installing port, and described room partition is set to the case
In vivo and the cabinet is divided into first room and second room, and described room partition is parallel with the rhizosphere access panel sets
It sets, far from the rhizosphere access panel, first room is used for placing normal soil sample, second room for first room
It carries disease germs soil sample in placement, the lower end of first room and second room is each provided with a filtrate pipe and is in communication with the outside；
Described room partition top is provided with for installing the mounting groove every root plate, and the mounting groove upper surface is provided with multiple the
One groove, described that root plate lower surface is provided with multiple second grooves, first groove and second groove correspond
And structure matches, corresponding first groove and second groove form one and lead root hole, it is each it is described lead root plate can
It is releasably installed on the lower section of first groove, and the root plate of leading is installed on described room partition close to the rhizosphere
The side of access panel, the rhizosphere sampler include multiple cutting blades disposed in parallel.
2. the rhizosphere sampler according to claim 1 for the research of continuous cropping soil distress mechanism, which is characterized in that use
In being symmetrical arranged on the two side plate inner walls of the cabinet for installing the rhizosphere access panel, there are two sample board slot, the sampling
Board slot is disposed in proximity to one end of the rhizosphere access panel, the sampling plate be installed in the sampling board slot after with described
Border access panel is arranged in parallel.
3. the rhizosphere sampler according to claim 1 for the research of continuous cropping soil distress mechanism, which is characterized in that institute
It states and is each provided with a limit plate every the two sides on root board ends top.
4. the rhizosphere sampler according to claim 3 for the research of continuous cropping soil distress mechanism, which is characterized in that institute
The two sides for stating root room partition upper end are each provided with a fixed knob, and the fixed knob is described every root plate for fastening.
5. the rhizosphere sampler according to claim 1 for the research of continuous cropping soil distress mechanism, which is characterized in that institute
It states one end to the one end close to the rhizosphere access panel for leading root hole by the separate rhizosphere access panel and tilts down setting.
6. the rhizosphere sampler according to claim 1 for the research of continuous cropping soil distress mechanism, which is characterized in that institute
It states root room partition and the side of the rhizosphere access panel is provided with multiple card slots, the card slot and first groove one are a pair of
It answers, and a card slot is located at the lower section of first groove, a card slot is for leading root described in installation one
Plate.
7. the rhizosphere sampler according to claim 6 for the research of continuous cropping soil distress mechanism, which is characterized in that institute
Stating and leading the cross section of root plate is V-shaped.
8. the rhizosphere sampler according to claim 1 for the research of continuous cropping soil distress mechanism, which is characterized in that institute
Stating rhizosphere sampler further includes operating stick, laser shot-light, bearing roller, bearing fixing rod, battery, battery cover, power knob, two
Away from gasket, the operating stick inner hollow forms battery compartment for a hold-doun nut and multiple controls, and the battery compartment is described for placing
Battery, the battery cover are set to above the battery compartment, and the laser shot-light is set to the lower part of the operating stick downside,
The power knob is set to the top of the operating stick upper side, and the battery is used to power to the laser shot-light, described
Power knob is used to control the opening and closing of the battery, and the bearing roller is fixed on the operating stick lower end, and the bearing is fixed
Bar fixing sleeve is set to inside the bearing roller, and the control is sheathed on outside the bearing fixing rod away from gasket and the cutting blade
Portion, the both ends of the bearing fixing rod are separately installed with the hold-doun nut, between two adjacent cutting blades
The control is provided with away from gasket.
9. a kind of such as the rhizosphere sampler of any of claims 1-8 studied for continuous cropping soil distress mechanism
Application method, which comprises the following steps:
Step 1 fills normal soil sample in first room Xiang Suoshu, and filling is carried disease germs soil sample in second room Xiang Suoshu, bankets and reaches
Root plate is led after certain altitude, described in installation, and by water planting transplantation of seedlings to first room, the root of Rooted Cuttings is led root across described
Hole, into it is described lead root plate after, lead root plate described in closure is fixed, continue earthing；
Step 2 checks root growth and incidence by described room access panel；
Step 3 cuts soil layer in the side close to described room access panel by sampling plate；
The cabinet is laid flat so that described room access panel is located above, described room access panel is opened, using institute by step 4
Rhizosphere sampler is stated to cut along root system；Root system is taken out, root table microorganism and root system plant sample are extracted；Using thin blade along
Separate soil extract sample.
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Inventor after: Wang Xianting Inventor after: Xu Jinggao Inventor after: Ma Jianfang Inventor after: Lin Zhao Inventor after: He Yingxiang Inventor after: Shao Peimin Inventor after: Wang Bin Inventor after: Chen Jianfeng Inventor after: Zhang Xiaomeng Inventor after: Zeng Lihong Inventor after: Zhang Jiali Inventor after: Zhang Zhiming Inventor after: Liu Rongjie Inventor after: Feng Qianyan Inventor before: Wang Xianting Inventor before: Xu Jinggao Inventor before: Ma Jianfang Inventor before: Lin Zhao Inventor before: He Yingxiang Inventor before: Shao Peimin Inventor before: Wang Bin Inventor before: Zeng Lihong Inventor before: Zhang Xiaomeng Inventor before: Chen Jianfeng Inventor before: Zhang Jiali Inventor before: Zhang Zhiming Inventor before: Liu Rongjie Inventor before: Feng Qianyan |
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