CN110487585B - Rhizosphere sampling device for continuous cropping soil disease mechanism research and application method - Google Patents
Rhizosphere sampling device for continuous cropping soil disease mechanism research and application method Download PDFInfo
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- CN110487585B CN110487585B CN201910891068.6A CN201910891068A CN110487585B CN 110487585 B CN110487585 B CN 110487585B CN 201910891068 A CN201910891068 A CN 201910891068A CN 110487585 B CN110487585 B CN 110487585B
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- 239000002689 soil Substances 0.000 title claims abstract description 69
- 238000005070 sampling Methods 0.000 title claims abstract description 58
- 201000010099 disease Diseases 0.000 title claims abstract description 26
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 26
- 230000007246 mechanism Effects 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title claims abstract description 22
- 238000009335 monocropping Methods 0.000 title claims abstract description 21
- 238000011160 research Methods 0.000 title claims abstract description 20
- 238000005192 partition Methods 0.000 claims abstract description 41
- 238000002955 isolation Methods 0.000 claims description 9
- 230000009471 action Effects 0.000 claims description 6
- 244000005700 microbiome Species 0.000 claims description 5
- 239000000706 filtrate Substances 0.000 claims description 4
- 238000009434 installation Methods 0.000 claims 2
- 238000005527 soil sampling Methods 0.000 abstract description 3
- 230000001360 synchronised effect Effects 0.000 abstract description 3
- 230000008569 process Effects 0.000 description 7
- 241000196324 Embryophyta Species 0.000 description 6
- 238000013461 design Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 239000010410 layer Substances 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 230000008506 pathogenesis Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 239000011229 interlayer Substances 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 206010011409 Cross infection Diseases 0.000 description 1
- 206010029803 Nosocomial infection Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 230000001863 plant nutrition Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01D—HARVESTING; MOWING
- A01D91/00—Methods for harvesting agricultural products
- A01D91/02—Products growing in the soil
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G9/00—Cultivation in receptacles, forcing-frames or greenhouses; Edging for beds, lawn or the like
- A01G9/02—Receptacles, e.g. flower-pots or boxes; Glasses for cultivating flowers
- A01G9/028—Multi-compartmented pots
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Abstract
The invention discloses a rhizosphere sampling device for continuous cropping soil disease mechanism research and an application method thereof, and relates to the technical field of rhizosphere soil sampling, wherein one side of a box body is provided with a mounting opening, a rhizosphere observation plate is detachably mounted on the mounting opening, and a root chamber partition plate is arranged in the box body and divides the box body into a first root chamber and a second root chamber; the root chamber baffle upper portion is provided with the mounting groove that is used for installing the root partition board, and the mounting groove upper surface is provided with a plurality of first recesses, and the root partition board lower surface is provided with a plurality of second recesses, and corresponding first recess and second recess form a root guide hole, and each root guide board detachably installs in the below of a first recess, and the root guide board installs in the root chamber baffle one side that is close to the rhizosphere observation board, and the rhizosphere sampler includes a plurality of parallel arrangement's cutting blade. The device and the application method can accurately position the diseased rhizosphere area and realize synchronous sampling research of root systems, rhizosphere soil and non-rhizosphere soil.
Description
Technical Field
The invention relates to the technical field of rhizosphere soil sampling, in particular to a rhizosphere sampling device for continuous cropping soil disease mechanism research and an application method thereof.
Background
The rhizosphere is an important area for nutrient and energy exchange between the crop root system and the soil, is the most active place of soil microorganisms, and is a specific area influenced by the growth of the crop root system and simultaneously the absorption and transportation of the crop nutrient. At present, rhizosphere micro-domain and period soil microorganism research is one of the focus of research of multiple disciplines such as soil science, plant nutrition science, agriculture and the like at home and abroad.
The conventional rhizosphere soil sampling method is mainly a human shaking separation method, but has the problems of nonuniform sampling quantity and low accuracy; the three-chamber multi-interlayer root box is adopted or closely adhered through an interlayer net for millimeter-level sampling, but the soil packing tightness has an influence on the rhizosphere spacing distance, and meanwhile, the sampling is a rhizosphere non-rhizosphere mixed sample, so that the action part of the root system cannot be accurately researched; in addition, a frozen slicing method is adopted, but the slicing thickness is not easy to control, the slicing can damage plant root systems, the rhizosphere root systems, rhizosphere soil and non-rhizosphere soil can not be sampled at the same time, and meanwhile, the quick freezing process can change the nutrient condition and the microbial activity in soil. At present, a root box device for researching a special precise positioning disease-causing rhizosphere area aiming at a soil continuous sitting obstacle mechanism is not reported.
Disclosure of Invention
In order to solve the technical problems, the invention provides a rhizosphere sampling device for continuous cropping soil disease mechanism research and an application method thereof, which are used for accurately positioning the diseased rhizosphere area and realizing synchronous sampling research of root systems, rhizosphere soil and non-rhizosphere soil.
In order to achieve the above object, the present invention provides the following solutions:
the invention provides a rhizosphere sampling device for continuous cropping soil disease mechanism research, which comprises a box body with an opening at the upper part, a rhizosphere observation plate, a root chamber partition plate, a root isolation plate, a sampling plate, a rhizosphere sampler and a plurality of root guide plates, wherein one side of the box body is provided with a mounting opening, the rhizosphere observation plate is detachably mounted on the mounting opening, the root chamber partition plate is arranged in the box body and divides the box body into a first root chamber and a second root chamber, the root chamber partition plate is arranged in parallel with the rhizosphere observation plate, the first root chamber is far away from the rhizosphere observation plate, the first root chamber is used for placing a normal soil sample, the second root chamber is used for placing a soil sample with bacteria, and the lower ends of the first root chamber and the second root chamber are respectively provided with a filtrate pipe communicated with the outside; the root chamber partition plate upper portion is provided with the mounting groove that is used for installing the root partition plate, the mounting groove upper surface is provided with a plurality of first recesses, the root partition plate lower surface is provided with a plurality of second recesses, first recess with second recess one-to-one and structure phase-match, corresponding first recess with the second recess forms a root guide hole, each root guide plate detachably install in one the below of first recess, just the root guide plate install in the root chamber partition plate is close to one side of root observation plate, the root sampler includes a plurality of parallel arrangement's cutting blade.
Preferably, two sampling plate grooves are symmetrically formed in the inner walls of the two side plates of the box body for installing the rhizosphere observation plate, the sampling plate grooves are formed in one end, close to the rhizosphere observation plate, of the box body, and the sampling plates are arranged in the sampling plate grooves and then are arranged in parallel with the rhizosphere observation plate.
Preferably, two limiting plates are respectively arranged on two sides of the upper parts of two ends of the root isolation plate.
Preferably, two sides of the upper end of the root chamber partition plate are respectively provided with a fixing knob, and the fixing knobs are used for fastening the root chamber partition plate.
Preferably, the root guide hole is arranged in a downward inclined manner from one end far away from the rhizosphere observation plate to one end close to the rhizosphere observation plate.
Preferably, a plurality of clamping grooves are formed in one side, close to the rhizosphere observation plate, of the root chamber partition plate, the clamping grooves correspond to the first grooves one by one, one clamping groove is located below one first groove, and one clamping groove is used for installing one root guide plate.
Preferably, the cross section of the root guide plate is V-shaped.
Preferably, the rhizosphere sampler further comprises an operating rod, a laser spotlight, a bearing roller, a bearing fixing rod, a battery cover, a power button, two fixing nuts and a plurality of distance control gaskets, wherein a battery bin is formed in the hollow mode inside the operating rod, the battery bin is used for accommodating the battery, the battery cover is arranged above the battery bin, the laser spotlight is arranged at the lower part of the lower side surface of the operating rod, the power button is arranged at the upper part of the upper side surface of the operating rod, the battery is used for supplying power to the laser spotlight, the power button is used for controlling the opening and closing of the battery, the bearing roller is fixed at the lower end of the operating rod, the bearing fixing rod is fixedly sleeved inside the bearing roller, the distance control gaskets and the cutting blades are sleeved outside the bearing fixing rod, one fixing nut is respectively arranged at two ends of the bearing fixing rod, and one distance control gasket is arranged between every two adjacent cutting blades.
The invention also provides an application method of the rhizosphere sampling device for continuous cropping soil disease mechanism research, which comprises the following steps:
firstly, filling a normal soil sample into the first root chamber, filling a bacteria-carrying soil sample into the second root chamber, installing the root guide plate after filling soil to a certain height, transplanting hydroponic seedlings into the first root chamber, enabling roots of the hydroponic seedlings to pass through the root guide holes, entering the root guide plate, closing and fixing the root guide plate, and continuously covering soil;
checking the growth and morbidity of the root system through the rhizosphere observation plate;
cutting a soil layer on one side close to the rhizosphere observation plate through a sampling plate;
fourthly, the box body is put flat so that the rhizosphere observation plate is positioned above, the rhizosphere observation plate is opened, and the rhizosphere sampler is adopted to cut along a root system; taking out the root system, and extracting microorganisms on the root surface and a root system plant sample; samples were extracted along the partitioned soil samples using a thin blade.
Compared with the prior art, the invention has the following technical effects:
the rhizosphere sampling device for continuous cropping soil disease mechanism research and the application method provided by the invention adopt the design of the isolated root chamber, the root system in the first root chamber grows normally, the root system in the second root chamber is ill, and the directional plant root system is ensured to be ill. Root system guiding is achieved through the root guiding holes and the root guiding plates, so that the root system in the second root chamber grows by attaching the rhizosphere observation plates, and the infection process and the prevention process of crop root system growth pathogens can be observed. By observing the dynamic process of rhizosphere disease, the critical area of root system disease is determined, the rhizosphere observation plate is opened, the rhizosphere sampler is used for realizing accurate directional sampling of the rhizosphere area, and meanwhile, the disease part, rhizosphere soil and non-rhizosphere soil are obtained. The design of the separated root guide holes is adopted, so that the disturbance or mechanical damage of the root system is avoided.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic structural diagram of a rhizosphere sampling device for continuous cropping soil disease mechanism study, which is provided by the invention;
FIG. 2 is a schematic structural view of a rhizosphere sampling device for continuous cropping soil disease mechanism study, provided by the invention, without a root isolation plate;
FIG. 3 is an enlarged view of a portion of a second root chamber of the present invention;
FIG. 4 is a schematic view of a first chamber component of the present invention;
FIG. 5 is a schematic view of a second root chamber assembly according to the present invention;
FIG. 6 is a schematic view of the structure of the root chamber partition and the root partition in the present invention;
FIG. 7 is a schematic view of a root guide plate according to the present invention;
FIG. 8 is a schematic diagram of a rhizosphere sampler according to the present invention;
FIG. 9 is an exploded view of the rhizosphere sampler of the present invention;
FIG. 10 is a schematic view of the present invention with a sampling plate installed;
FIG. 11 is a schematic diagram of the rhizosphere sampler of the present invention;
FIG. 12 is an enlarged view of a portion of the rhizosphere sampler of the present invention when sampling.
Reference numerals illustrate: 1. a first root chamber assembly; 2. a second root chamber assembly; 3. a root chamber partition; 4. a root isolation plate; 5. a limiting plate; 6. a rhizosphere observation plate; 7. sampling plate grooves; 8. a filtrate pipe; 9. a root guide plate; 10. fixing a knob; 11. a first groove; 12. a second groove; 13. a clamping groove; 14. sampling a template; 15. an operation lever; 16. a power button; 17. a battery cover; 18. a laser spot lamp; 19. bearing rollers; 20. a bearing fixing rod; 21. a cutting blade; 22. distance control gaskets; 23. a fixed screw cap; 24. the light beam is directed.
Description of the embodiments
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The invention aims to provide a rhizosphere sampling device for continuous cropping soil disease mechanism research and an application method thereof, which are used for accurately positioning a disease rhizosphere region and realizing synchronous sampling research of root systems, rhizosphere soil and non-rhizosphere soil.
In order that the above-recited objects, features and advantages of the present invention will become more readily apparent, a more particular description of the invention will be rendered by reference to the appended drawings and appended detailed description.
As shown in fig. 1 to 9, the present embodiment provides a rhizosphere sampling device for continuous cropping soil disease mechanism study, which comprises a box body with an opening at the upper part, a rhizosphere observation plate 6, a root chamber partition plate 3, a root partition plate 4, a sampling plate 14, a rhizosphere sampler and a plurality of root guide plates 9, wherein one side of the box body is provided with a mounting opening, the rhizosphere observation plate 6 is detachably mounted on the mounting opening, the root chamber partition plate 3 is arranged in the box body and divides the box body into a first root chamber and a second root chamber, the root chamber partition plate 3 is arranged in parallel with the rhizosphere observation plate 6, the first root chamber is far away from the rhizosphere observation plate 6, the first root chamber is used for placing normal soil samples, the second root chamber is used for placing bacteria soil samples, and the lower ends of the first root chamber and the second root chamber are respectively provided with a filtrate pipe 8 which is communicated with the outside; the root chamber baffle 3 upper portion is provided with the mounting groove that is used for installing the root baffle 4, the mounting groove upper surface is provided with a plurality of first recesses 11, root baffle 4 lower surface is provided with a plurality of second recesses 12, first recess 11 and second recess 12 one-to-one and structure phase-match, corresponding first recess 11 and second recess 12 form a root guide hole, each root guide plate 9 detachably installs in the below of a first recess 11, and root guide plate 9 installs in the root chamber baffle 3 one side that is close to rhizosphere observation board 6, the rhizosphere sampler includes a plurality of parallel arrangement's cutting blade 21.
In this embodiment, the design of the isolated root chamber is adopted, the root system in the first root chamber grows normally, and the root system in the second root chamber attacks, so that the directional attack of the plant root system is ensured. Root guiding is achieved through the arrangement of the root guiding holes and the root guiding plates 9, so that the root system in the second root chamber grows by attaching the rhizosphere observation plate 6, and the infection process and the prevention process of crop root system growth pathogens can be observed. Through observing the rhizosphere pathogenesis dynamic process, determining a root system pathogenesis key area, opening a rhizosphere observation plate 6, and applying rhizosphere samplers to realize the precise directional sampling of the rhizosphere area of the research of soil continuous-sitting obstacle mechanism, the rhizosphere samplers with a plurality of cutting blades 21 can simultaneously obtain the pathogenesis part, rhizosphere soil and non-rhizosphere soil. The design of the separated root guide holes is adopted, so that the disturbance or mechanical damage of the root system is avoided. By using the cutting type sampling plate 14, a near rhizosphere growth soil layer can be obtained, so that rhizosphere soil samples can be conveniently taken.
In this embodiment, the rhizosphere observation plate 6 is a transparent organic glass plate, and two sides of the rhizosphere observation plate 6 are mounted on two side plates at the mounting opening of the box body through screws and nuts.
In this embodiment, the case includes a first root chamber component 1 and a second root chamber component 2, two ends of the first root chamber component 1 and the second root chamber component 2 are abutted and the root chamber partition 3 is clamped in the middle, and then two sides of the first root chamber component 1 and the second root chamber component 2 are fixed by adopting screws and nuts.
In order to guide the sampling plate 14, two sampling plate grooves 7 are symmetrically formed in the inner walls of two side plates of the box body for installing the rhizosphere observation plate 6, the sampling plate grooves 7 are formed in one end, close to the rhizosphere observation plate 6, of the sampling plate 14, and the sampling plate 14 is arranged in the sampling plate grooves 7 and then is arranged in parallel with the rhizosphere observation plate 6. In this embodiment, the distance between the sampling plate groove 7 and the rhizosphere observation plate 6 is 0.4cm, and the sampling plate 14 is a stainless steel plate with a thickness of 1 mm.
As shown in fig. 3 and 6, in order to limit the forward and backward displacement of the root partition plate 4, two limiting plates 5 are provided on both sides of the upper portions of both ends of the root partition plate 4. In order to limit the up-and-down displacement of the root partition plate 4, two sides of the upper end of the root compartment partition plate 3 are respectively provided with a fixing knob 10, and the fixing knob 10 is used for fastening the root partition plate 4. When the device is used, after the root isolation plate 4 is placed in the mounting groove, the limit plate 5 limits the root isolation plate in the front-back direction, and the rotary fixing knob 10 limits the root isolation plate 4 in the upper-lower direction, so that the root isolation plate 4 is firmly mounted on the root chamber partition plate 3, soil leakage and root penetration between the first root chamber and the second root chamber are prevented, and observation and sampling are affected.
Specifically, the root guide hole is arranged by one end far away from the rhizosphere observation plate 6 to one end close to the rhizosphere observation plate 6 in a downward inclined manner, so that a height drop is formed, and cross infection of soil germs between the first root chamber and the second root chamber is avoided, and the test result is influenced.
Specifically, a plurality of clamping grooves 13 are formed in one side, close to the rhizosphere observation plate 6, of the root chamber partition plate 3, the clamping grooves 13 are in one-to-one correspondence with the first grooves 11, one clamping groove 13 is located below one first groove 11, and one clamping groove 13 is used for installing one root guide plate 9. In this embodiment, the cross section of the root guide plate 9 is V-shaped.
As shown in fig. 8 and 9, the rhizosphere sampler further comprises an operation rod 15, a laser spotlight 18, a bearing roller 19, a bearing fixing rod 20, a battery cover 17, a power button 16, two fixing nuts 23 and a plurality of distance control gaskets 22, a battery bin is formed in the operation rod 15, the battery bin is used for accommodating the battery, the battery cover 17 is arranged above the battery bin, the laser spotlight 18 is arranged at the lower part of the lower side surface of the operation rod 15, the power button 16 is arranged at the upper part of the upper side surface of the operation rod 15, the battery is used for supplying power to the laser spotlight 18, the power button 16 is used for controlling the opening and closing of the battery, the bearing roller 19 is fixed at the lower end of the operation rod 15, the bearing fixing rod 20 is fixedly sleeved in the bearing roller 19, the distance control gaskets 22 and the cutting blades 21 are sleeved outside the bearing fixing rod 20, two ends of the bearing fixing rod 20 are respectively provided with one fixing nut 23, one distance control gasket 22 is arranged between two adjacent cutting blades 21, the distance between the two adjacent cutting blades 21 can be adjusted by changing the distance control gaskets 22 with different thicknesses, and the cutting distance between the two adjacent cutting blades 21 can be adjusted according to actual requirements.
When the cutting machine is used, the laser spotlight 18 is started through the power button 16, and the laser spotlight 18 emits the guide beam 24 to guide the cutting direction, so that the cutting and sampling are more accurate. By using the rhizosphere sampler designed in the embodiment, the soil with different distances from the root system is accurately sampled, the distance from the root system is determined, and compared with a liquid nitrogen freezing cutting method, the rhizosphere sampler has the advantages that the target is clear and the plant body sample is not damaged.
In this embodiment, 3 cutting blades 21 are mounted on each side of the bearing roller 19.
The embodiment also provides an application method of the rhizosphere sampling device for continuous cropping soil disease mechanism research, which comprises the following steps:
firstly, filling a normal soil sample into a first root chamber, filling a bacteria-carrying soil sample into a second root chamber, installing a root guide plate 9 after filling soil to a certain height, transplanting hydroponic seedlings into the first root chamber, enabling the hydroponic seedlings to grow consistently and have no bacteria, enabling roots of the hydroponic seedlings to pass through root guide holes, closing and fixing the root guide plate 9 after entering the root guide plate 9, and continuing earthing;
checking the growth and morbidity of the root system through a rhizosphere observation plate 6;
step three, as shown in fig. 10, cutting a soil layer of 0.4cm on the side close to the rhizosphere observation plate 6 by the sampling plate 14;
step four, as shown in fig. 11 and 12, the box body is put flat so that the rhizosphere observation plate 6 is positioned above, the rhizosphere observation plate 6 is opened, and a rhizosphere sampler is adopted to cut along the root system; taking out the root system, and extracting microorganisms on the root surface and a root system plant sample; samples were extracted along the partitioned soil samples using a thin blade.
The principles and embodiments of the present invention have been described in this specification with reference to specific examples, the description of which is only for the purpose of aiding in understanding the method of the present invention and its core ideas; also, it is within the scope of the present invention to be modified by those of ordinary skill in the art in light of the present teachings. In view of the foregoing, this description should not be construed as limiting the invention.
Claims (8)
1. The rhizosphere sampling device for continuous cropping soil disease mechanism research is characterized by comprising a box body with an opening at the upper part, a rhizosphere observation plate, a root chamber partition board, a root partition board, a sampling board, a rhizosphere sampler and a plurality of root guide boards, wherein one side of the box body is provided with an installation opening, the rhizosphere observation plate is detachably installed on the installation opening, the root chamber partition board is arranged in the box body and divides the box body into a first root chamber and a second root chamber, the root chamber partition board is arranged in parallel with the rhizosphere observation board, the first root chamber is far away from the rhizosphere observation plate, the first root chamber is used for placing a normal soil sample, the second root chamber is used for placing a bacteria-carrying soil sample, and the lower ends of the first root chamber and the second root chamber are respectively provided with a filtrate pipe which is communicated with the outside; the root chamber partition plate comprises a root chamber partition plate, wherein the upper part of the root chamber partition plate is provided with a mounting groove for mounting the root partition plate, the upper surface of the mounting groove is provided with a plurality of first grooves, the lower surface of the root partition plate is provided with a plurality of second grooves, the first grooves are in one-to-one correspondence with the second grooves and are matched in structure, the corresponding first grooves and second grooves form a root guide hole, each root guide plate is detachably mounted below one first groove, the root guide plate is mounted on one side, close to the rhizosphere observation plate, of the root chamber partition plate, and the rhizosphere sampler comprises a plurality of cutting blades which are arranged in parallel; the rhizosphere sampler still includes action bars, laser shot-light, bearing gyro wheel, bearing dead lever, battery cover, power button, two fixation nut and a plurality of accuse apart from the gasket, the inside cavity of action bars forms the battery compartment, the battery compartment is used for placing the battery, the battery cover set up in battery compartment top, the laser shot-light set up in the lower part of action bars downside, power button set up in the upper portion of action bars upside, the battery is used for to the laser shot-light power supply, power button is used for controlling the switching of battery, the bearing gyro wheel is fixed in the action bars lower extreme, the fixed cover of bearing dead lever is located inside the bearing gyro wheel, accuse apart from the gasket with cutting blade cover is located the bearing dead lever is outside, one is installed respectively at the both ends of bearing dead lever fixation nut, adjacent two be provided with one between the cutting blade accuse apart from the gasket.
2. The rhizosphere sampling device for continuous cropping soil disease mechanism study according to claim 1, wherein two sampling plate grooves are symmetrically formed in inner walls of two side plates of the box body for installing the rhizosphere observation plate, the sampling plate grooves are formed in one end close to the rhizosphere observation plate, and the sampling plate is arranged in parallel with the rhizosphere observation plate after being installed in the sampling plate grooves.
3. The rhizosphere sampling device for continuous cropping soil disease mechanism study according to claim 1, wherein two limiting plates are respectively arranged on two sides of the upper parts of two ends of the root isolation plate.
4. The rhizosphere sampling device for continuous cropping soil disease mechanism research according to claim 3, wherein two sides of the upper end of the root chamber partition plate are respectively provided with a fixing knob, and the fixing knobs are used for fastening the root partition plate.
5. The rhizosphere sampling device for continuous cropping soil disease mechanism studies according to claim 1, wherein the root guide hole is disposed obliquely downward from an end far from the rhizosphere observation plate to an end near the rhizosphere observation plate.
6. The rhizosphere sampling device for continuous cropping soil disease mechanism research according to claim 1, wherein a plurality of clamping grooves are formed in one side, close to the rhizosphere observation plate, of the root chamber partition plate, the clamping grooves are in one-to-one correspondence with the first grooves, one clamping groove is located below one first groove, and one clamping groove is used for installing one root guide plate.
7. The rhizosphere sampling device for continuous cropping soil disease mechanism research according to claim 6, wherein the cross section of the root guide plate is V-shaped.
8. A method of using the rhizosphere sampling device for continuous cropping soil disease mechanism studies according to any one of claims 1-7, comprising the steps of:
firstly, filling a normal soil sample into the first root chamber, filling a bacteria-carrying soil sample into the second root chamber, installing the root guide plate after filling soil to a certain height, transplanting hydroponic seedlings into the first root chamber, enabling roots of the hydroponic seedlings to pass through the root guide holes, entering the root guide plate, closing and fixing the root guide plate, and continuously covering soil;
checking the growth and morbidity of the root system through the rhizosphere observation plate;
cutting a soil layer on one side close to the rhizosphere observation plate through a sampling plate;
fourthly, the box body is put flat so that the rhizosphere observation plate is positioned above, the rhizosphere observation plate is opened, and the rhizosphere sampler is adopted to cut along a root system; taking out the root system, and extracting microorganisms on the root surface and a root system plant sample; samples were extracted along the partitioned soil samples using a thin blade.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910891068.6A CN110487585B (en) | 2019-09-20 | 2019-09-20 | Rhizosphere sampling device for continuous cropping soil disease mechanism research and application method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910891068.6A CN110487585B (en) | 2019-09-20 | 2019-09-20 | Rhizosphere sampling device for continuous cropping soil disease mechanism research and application method |
Publications (2)
| Publication Number | Publication Date |
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| CN110487585A CN110487585A (en) | 2019-11-22 |
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