CN106069756A - A kind of quick breeding method for tissue culture spending Herba Phyllanthi Urinariae in vain - Google Patents
A kind of quick breeding method for tissue culture spending Herba Phyllanthi Urinariae in vain Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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Abstract
This application discloses a kind of quick breeding method for tissue culture spending Herba Phyllanthi Urinariae in vain, choose the blade spending Herba Phyllanthi Urinariae in vain the outer implant as group training；Form seedling by blade surface bulbil inducing culture, or form seedling by Callus of Leaf inducing culture, adventitious bud induction culture, root culture；After seedling grows adventitious root, carry out tissue cultured seedling rooting culture, the most normally cultivate.The application spends the quick breeding method for tissue culture of Herba Phyllanthi Urinariae in vain; solve and use the problem that underground propagation by corm exists regeneration difficulty, bulb reproduction coefficient is low; there is remarkable advantage easy and simple to handle, that growth coefficient is high; not limited by area, season, weather, the industrial massive that can realize high quality seedling produces.
Description
Technical field
The application belongs to technical field of plant propagation, specifically, relates to a kind of tissue culture spending Herba Phyllanthi Urinariae in vain
Method for quickly breeding.
Background technology
Spending Herba Phyllanthi Urinariae (Ornithogalum thyroides) in vain is Liliaceae Ornithogalum
The perennial bulb flowers of (Ornithogalum spp), high 25～50 centimetres, tunicated bulb circle is smooth, and ornamental value is high.
Leaf base is grown thickly, lanceolar and grow, top tool long-tail, bottle green, stockless.Spending small-sized, raceme is tight, pyramid, raw
In the scape top of pumping from leafage, above little Hua more than 25, flower-shape cup-shaped, pattern white, petal central authorities redgreen striped.
Not only its flower and bulb have higher ornamental value, can be widely applied to afforestation and as Fresh Cutting flower,
And chemical composition analysis shows, wherein contain the secondary metabolites such as steroline, cholestane glucosides, have potential medicinal
And economic worth.
Along with economic society and the development of gardens cause, flowering bulb because have that abundant species, pattern be gorgeous, the florescence relatively
Long, cultivation easily, feature and the advantage such as strong adaptability, in gardens, application is more and more, can be widely used as cut-flower, quilt and basin
Flowers etc., also have higher medicinal and economic worth.Herba Phyllanthi Urinariae's platymiscium, as Liliaceae perennial bulb flowers, has
Kind more than 150, because it has higher ornamental value, and higher medical value, is more and more attracted attention.
But the most out of office the surviving of breeding of flowering bulb is in vitro, it is substantially the bulb using underground, generally
The outstanding problem such as there is regeneration difficulty, bulb propagation coefficient is low and germplasm innovation is difficult, Herba Phyllanthi Urinariae's platymiscium is no exception,
Become the bottleneck of its research and development of restriction.
Summary of the invention
In view of this, the problem that the application exists regeneration difficulty for employing underground propagation by corm, bulb reproduction coefficient is low,
Provide a kind of quick breeding method for tissue culture spending Herba Phyllanthi Urinariae in vain, there is high notable of easy and simple to handle, growth coefficient
Advantage, the industrial massive that can realize high quality seedling produces.
In order to solve above-mentioned technical problem, this application discloses a kind of tissue-culturing quick-propagation spending Herba Phyllanthi Urinariae in vain
Method, chooses the blade spending Herba Phyllanthi Urinariae in vain the outer implant as group training；Formed by blade surface bulbil inducing culture
Seedling, or form seedling by Callus of Leaf inducing culture, adventitious bud induction culture, root culture；Treat that seedling grows
After adventitious root, carry out tissue cultured seedling rooting culture, the most normally cultivate.
Further, following steps are specifically included:
Step 1, the selection of outer implant and process
Choosing and spending the blade of Herba Phyllanthi Urinariae in vain is parent material, and the blade collected is cut into the leaf section of 2～4cm, makees
Outer implant for group training；
Step 2, carries out induced synthesis seedling to blade
Outer implant through surface sterilization is inoculated on bulbil inducing culture MS+TDZ2.0mg/L and trains at illumination
Support, after blade surface gradually forms complete root system, root system is inserted in culture medium the seedling becoming new；
Or, the outer implant through surface sterilization is inoculated in callus inducing medium MS+2,4D2.0mg/L is enterprising
Row light culture, treats to form white or flaxen callus at paddle cutout, is then inoculated in adventitious bud induction culture base MS+
6BA2.0mg/L, is placed at illumination and cultivates, and callus gradually forms adventitious bud, then is transferred to root media MS+
On IBA1.0mg/L, after 20～30d, seedling of taking root becomes new seedling；
Step 3, tissue cultured seedling rooting culture
After the adventitious root that seedling grows 3～5 2～3cm, carry out seedling exercising, then tissue cultured seedling is transplanted to culture matrix
In carry out slow Seedling, more normally cultivate.
Further, cultivation temperature is 25 DEG C.
Further, light application time is 12～14h/d, and intensity of illumination is 40～60u.Mol.m-2.s-1。
Further, bulbil induction, callus induction, Induce aerosor and root culture, minimal medium used
Being MS culture medium, dosage of sucrose is 20～40g/L, coagulator be agar powder, consumption be 6～7g/L, medium pH is 5.8.
Further, described seedling exercising particularly as follows: first group training chamber opening bottle cap seedling exercising 3 days, be then transported to greenhouse or
Cool canopy, then seedling exercising 3 days.
Further, described culture matrix is made up of with turfy soil 3:1 in mass ratio sandy loam.
Further, within the water content 25% of described culture matrix, relative air humidity 80～90%.
Compared with prior art, the application can obtain and include techniques below effect:
(1) the application is by choosing the blade spending Herba Phyllanthi Urinariae in vain the outer implant as group training；Pass through blade surface
Bulbil inducing culture forms seedling, or is formed by Callus of Leaf inducing culture, adventitious bud induction culture, root culture
Seedling；After seedling grows adventitious root, carry out tissue cultured seedling rooting culture, the most normally cultivate, solve employing underground bulb numerous
Grow and there is the problem that regeneration is difficult, bulb reproduction coefficient is low, there is remarkable advantage easy and simple to handle, that growth coefficient is high, not by ground
District, season, weather limit, and the industrial massive that can realize high quality seedling produces.
(2) cutting blade from maternal plant, be outer implant with blade, a blade forms multiple bulbil, breeding coefficient reaches 3
～10, it is tens times even tens times of general modes of reproduction, and less to plant damage itself, and maternal plant can be with continued growth
And utilization, it is achieved sustainable production and development.
(3) foundation spends the high-efficiency regeneration system of Herba Phyllanthi Urinariae in vain, for spending the industrialization development of Herba Phyllanthi Urinariae, foundation in vain
Molecular breeding platform and Germplasm enhancement etc. provide good technical support and system to support；Herba Phyllanthi Urinariae is spent in vain for improving
Genetic conversion system, set up the platform of molecular breeding, from molecular level, it carried out genetic improvement, further investigation develops
Lay a good foundation.
(4) the application can be also the plant useful thinking of offer and the reference of other flowering bulbs and regeneration difficulty.
Certainly, the arbitrary product implementing the application it is not absolutely required to reach all the above technique effect simultaneously.
Accompanying drawing explanation
Accompanying drawing described herein is used for providing further understanding of the present application, constitutes the part of the application, this Shen
Schematic description and description please is used for explaining the application, is not intended that the improper restriction to the application.In the accompanying drawings:
Fig. 1 is the tissue-culturing quick-propagation process comparison diagram that the embodiment of the present application spends Herba Phyllanthi Urinariae in vain；In figure, A is
Spending Herba Phyllanthi Urinariae's blade in vain, B forms multiple bulbil for spending Herba Phyllanthi Urinariae's blade surface in vain, C be blade surface bulbil by
Gradually sprouting, D is that bulbil is taken root seedling, and E is that blade produces callus and callus differentiates adventitious bud, and F is for spending brave eye in vain
Rohdea japonica Roth kind seedling rooting, G forms bud for spending Herba Phyllanthi Urinariae in vain, and H is bloomed for spending Herba Phyllanthi Urinariae in vain.
Detailed description of the invention
Presently filed embodiment is described in detail, thereby to the application how application technology hands below in conjunction with embodiment
Section solves technical problem and reaches the process that realizes of technology effect and can fully understand and implement according to this.
The present invention spends the quick breeding method for tissue culture of Herba Phyllanthi Urinariae in vain, specifically includes following steps:
Step 1, the selection of outer implant and process
Choosing and spending the blade of Herba Phyllanthi Urinariae in vain is parent material, and the blade collected is cut into the leaf section of 2～4cm, makees
Outer implant for group training；
Step 2, carries out induced synthesis seedling to blade
Step 2-1, is transferred to the leaf section through surface sterilization in aseptic inoculation dish, blots leaf section surface with aseptic filter paper
Moisture；
Specifically, described sterilization cleans 30min, period particularly as follows: Herba Phyllanthi Urinariae's leaf section liquid detergent solution will be spent in vain
Constantly being gently mixed, then flowing water rinses 30～60min, by above-mentioned cleaned spending in vain on the most sterilized superclean bench
Herba Phyllanthi Urinariae's leaf section is transferred in sterile beaker, and with alcohol-pickled 30 that volumetric concentration is 75%～60s, period constantly rocks
Beaker, pours out ethanol, then soaks 3～5min with the mercuric chloride of mass concentration 0.1%, constantly rock gently, pour out liter during immersion
After hydrargyrum, by sterile water wash through the blade more than 3 times of sterilization.
Step 2-2, keeps flat or inserts bulbil inducing culture MS+TDZ2.0mg/L (TDZ: thiophene benzene by the leaf section after sterilization
Grand) on carry out the induction of bulbil, as shown in Fig. 1 (A), cultivation temperature 25 DEG C, light application time is 12～14h/d, and intensity of illumination is
40～60u.Mol.m-2.s-1；20d rear blade surface forms small particles, and as shown in Fig. 1 (B), small particles gradually turns green, after
After 20d, small particles becomes green ball, and as shown in Fig. 1 (C), green ball is slowly taken root, and eventually forms complete root system, such as Fig. 1 (D) institute
Show, root system is inserted in culture medium the seedling becoming new, be i.e. " take root on leaf, land seedling "；
Or, the leaf section after sterilization is inoculated in callus inducing medium MS+2,4D2.0mg/L (2,4D:2,4-bis-
Chlorophenoxyacetic acid) on be placed in the induction carrying out callus at dark, temperature 25 DEG C, at paddle cutout, form white after 20d
Or flaxen callus；Then the callus induced is transferred to adventitious bud induction culture base MS+6BA2.0mg/L
(6BA: benayl aminopurine) carries out the induction of adventitious bud, cultivation temperature 25 DEG C, and light application time is 12～14h/d, and intensity of illumination is
40～60u.Mol.m-2.s-1, after 20d, callus gradually forms adventitious bud (blade), as shown in Fig. 1 (E)；To induce again
Adventitious bud be transferred to carry out on root media MS+IBA1.0mg/L (IBA: indolebutyric acid) induction of adventitious root, 20～30d
After, seedling of taking root becomes new seedling, as shown in Fig. 1 (F)；
Wherein, bulbil induction, callus induction, Induce aerosor and root culture, minimal medium used is
MS culture medium, dosage of sucrose is 20～40g/L, coagulator be agar powder, consumption be 6～7g/L, medium pH is 5.8.
Step 3, tissue cultured seedling rooting culture
After the adventitious root that seedling grows 3～5 2～3cm, carry out seedling exercising, then the agar on tissue cultured seedling surface cleaned,
Being transplanted in culture matrix carry out slow Seedling, as shown in Fig. 1 (G), tissue cultured seedling is transplanted in basin and is formed bud, more normally cultivates,
As shown in Fig. 1 (H), Herba Phyllanthi Urinariae's indefinite inflorescence of spending in vain of breeding opens one month the most successively.
Described seedling exercising is particularly as follows: first at group training chamber opening bottle cap seedling exercising 3d, be then transported to greenhouse or cool canopy, then seedling exercising
3d。
Preferably, culture matrix is made up of with turfy soil 3:1 in mass ratio sandy loam, the water content 25% of culture matrix with
In, relative air humidity 80～90%.
Embodiment 1
Step 1, the selection of outer implant and process
Choosing and spending the blade of Herba Phyllanthi Urinariae in vain is parent material, the blade collected is cut into the leaf section of 3cm, as group
The outer implant of training；
Step 2, carries out induced synthesis seedling to blade
Step 2-1, is transferred to the leaf section through surface sterilization in aseptic inoculation dish, blots leaf section surface with aseptic filter paper
Moisture；
Step 2-2, the leaf section after sterilizing keeps flat or inserts and carries out bulbil on bulbil inducing culture MS+TDZ2.0mg/L
Induction, cultivation temperature 25 DEG C, light application time is 14h/d, and intensity of illumination is 40u.Mol.m-2.s-1；Treat blade surface gradually shape
After becoming complete root system, root system is inserted in culture medium the seedling becoming new；
Minimal medium used is MS culture medium, and dosage of sucrose is 30g/L, coagulator be agar powder, consumption be 6.5g/
L, medium pH is 5.8.
Step 3, tissue cultured seedling rooting culture
After the adventitious root that seedling grows 3 3cm, carry out seedling exercising, then the agar on tissue cultured seedling surface is cleaned, be transplanted to
Carrying out slow Seedling in culture matrix, culture matrix is made up of with turfy soil 3:1 in mass ratio sandy loam, the water content of culture matrix
Within 25%, relative air humidity 80%, more normally cultivate.
Embodiment 2
Step 1, the selection of outer implant and process
Choosing and spending the blade of Herba Phyllanthi Urinariae in vain is parent material, the blade collected is cut into the leaf section of 2cm, as group
The outer implant of training；
Step 2, carries out induced synthesis seedling to blade
Step 2-1, is transferred to the leaf section through surface sterilization in aseptic inoculation dish, blots leaf section surface with aseptic filter paper
Moisture；
Step 2-2, the leaf section after sterilizing keeps flat or inserts and carries out bulbil on bulbil inducing culture MS+TDZ2.0mg/L
Induction, cultivation temperature 25 DEG C, light application time is 13h/d, and intensity of illumination is 50u.Mol.m-2.s-1；Treat blade surface gradually shape
After becoming complete root system, root system is inserted in culture medium the seedling becoming new；
Minimal medium used is MS culture medium, and dosage of sucrose is 20g/L, coagulator be agar powder, consumption be 6g/L,
Medium pH is 5.8.
Step 3, tissue cultured seedling rooting culture
After the adventitious root that seedling grows 4 3cm, carry out seedling exercising, then the agar on tissue cultured seedling surface is cleaned, be transplanted to
Carrying out slow Seedling in culture matrix, culture matrix is made up of with turfy soil 3:1 in mass ratio sandy loam, the water content of culture matrix
Within 25%, relative air humidity 85%, more normally cultivate.
Embodiment 3
Step 1, the selection of outer implant and process
Choosing and spending the blade of Herba Phyllanthi Urinariae in vain is parent material, the blade collected is cut into the leaf section of 4cm, as group
The outer implant of training；
Step 2, carries out induced synthesis seedling to blade
Step 2-1, is transferred to the leaf section through surface sterilization in aseptic inoculation dish, blots leaf section surface with aseptic filter paper
Moisture；
Step 2-2, the leaf section after sterilizing keeps flat or inserts and carries out bulbil on bulbil inducing culture MS+TDZ2.0mg/L
Induction, cultivation temperature 25 DEG C, light application time is 12h/d, and intensity of illumination is 60u.Mol.m-2.s-1；Treat blade surface gradually shape
After becoming complete root system, root system is inserted in culture medium the seedling becoming new；
Minimal medium used is MS culture medium, and dosage of sucrose is 40g/L, coagulator be agar powder, consumption be 7g/L,
Medium pH is 5.8.
Step 3, tissue cultured seedling rooting culture
After the adventitious root that seedling grows 5 2cm, carry out seedling exercising, then the agar on tissue cultured seedling surface is cleaned, be transplanted to
Carrying out slow Seedling in culture matrix, culture matrix is made up of with turfy soil 3:1 in mass ratio sandy loam, the water content of culture matrix
Within 25%, relative air humidity 90%, more normally cultivate.
Embodiment 4
Step 1, the selection of outer implant and process
Choosing and spending the blade of Herba Phyllanthi Urinariae in vain is parent material, the blade collected is cut into the leaf section of 3cm, as group
The outer implant of training；
Step 2, carries out induced synthesis seedling to blade
Step 2-1, is transferred to the leaf section through surface sterilization in aseptic inoculation dish, blots leaf section surface with aseptic filter paper
Moisture；
Step 2-2, is inoculated in callus inducing medium MS+2 by the leaf section after sterilization, 4D2.0mg/L is placed in black
Dark place carries out the induction of callus, temperature 25 DEG C；Then the callus induced is transferred to adventitious bud induction culture base
MS+6BA2.0mg/L carries out the induction of adventitious bud, cultivation temperature 25 DEG C, and light application time is 14h/d, and intensity of illumination is
40u.Mol.m-2.s-1；The adventitious bud induced is transferred on root media MS+IBA1.0mg/L carry out adventitious root again
Induction, after 20～30d, seedling of taking root becomes new seedling；
Minimal medium used is MS culture medium, and dosage of sucrose is 30g/L, and coagulator is that agar powder, consumption are
6.5g/L, medium pH is 5.8.
Step 3, tissue cultured seedling rooting culture
After the adventitious root that seedling grows 3 3cm, carry out seedling exercising, then the agar on tissue cultured seedling surface is cleaned, be transplanted to
Carrying out slow Seedling in culture matrix, culture matrix is made up of with turfy soil 3:1 in mass ratio sandy loam, the water content of culture matrix
Within 25%, relative air humidity 80%, more normally cultivate.
Embodiment 5
Step 1, the selection of outer implant and process
Choosing and spending the blade of Herba Phyllanthi Urinariae in vain is parent material, the blade collected is cut into the leaf section of 2cm, as group
The outer implant of training；
Step 2, carries out induced synthesis seedling to blade
Step 2-1, is transferred to the leaf section through surface sterilization in aseptic inoculation dish, blots leaf section surface with aseptic filter paper
Moisture；
Step 2-2, is inoculated in callus inducing medium MS+2 by the leaf section after sterilization, 4D2.0mg/L is placed in black
Dark place carries out the induction of callus, temperature 25 DEG C；Then the callus induced is transferred to adventitious bud induction culture base
MS+6BA2.0mg/L carries out the induction of adventitious bud, cultivation temperature 25 DEG C, and light application time is 13h/d, and intensity of illumination is
50u.Mol.m-2.s-1；The adventitious bud induced is transferred on root media MS+IBA1.0mg/L carry out adventitious root again
Induction, after 20～30d, seedling of taking root becomes new seedling；
Minimal medium used is MS culture medium, and dosage of sucrose is 20g/L, coagulator be agar powder, consumption be 6g/
L, medium pH is 5.8.
Step 3, tissue cultured seedling rooting culture
After the adventitious root that seedling grows 4 3cm, carry out seedling exercising, then the agar on tissue cultured seedling surface is cleaned, be transplanted to
Carrying out slow Seedling in culture matrix, culture matrix is made up of with turfy soil 3:1 in mass ratio sandy loam, the water content of culture matrix
Within 25%, relative air humidity 85%, more normally cultivate.
Embodiment 6
Step 1, the selection of outer implant and process
Choosing and spending the blade of Herba Phyllanthi Urinariae in vain is parent material, the blade collected is cut into the leaf section of 4cm, as group
The outer implant of training；
Step 2, carries out induced synthesis seedling to blade
Step 2-1, is transferred to the leaf section through surface sterilization in aseptic inoculation dish, blots leaf section surface with aseptic filter paper
Moisture；
Step 2-2, is inoculated in callus inducing medium MS+2 by the leaf section after sterilization, 4D2.0mg/L is placed in black
Dark place carries out the induction of callus, temperature 25 DEG C；Then the callus induced is transferred to adventitious bud induction culture base
MS+6BA2.0mg/L carries out the induction of adventitious bud, cultivation temperature 25 DEG C, and light application time is 12h/d, and intensity of illumination is
60u.Mol.m-2.s-1；The adventitious bud induced is transferred on root media MS+IBA1.0mg/L carry out adventitious root again
Induction, after 20～30d, seedling of taking root becomes new seedling；
Minimal medium used is MS culture medium, and dosage of sucrose is 40g/L, coagulator be agar powder, consumption be 7g/
L, medium pH is 5.8.
Step 3, tissue cultured seedling rooting culture
After the adventitious root that seedling grows 5 2cm, carry out seedling exercising, then the agar on tissue cultured seedling surface is cleaned, be transplanted to
Carrying out slow Seedling in culture matrix, culture matrix is made up of with turfy soil 3:1 in mass ratio sandy loam, the water content of culture matrix
Within 25%, relative air humidity 90%, more normally cultivate.
Using said method to cultivate breeding to spending Herba Phyllanthi Urinariae's leaf tissue in vain, breeding coefficient reaches 3～10, is the most numerous
Grow mode tens times even tens times, and less to plant damage itself, and maternal plant can be with continued growth and utilization, it is achieved can
Continuous production and development, and do not limited by area, season, weather, the industrial massive that can realize high quality seedling produces.
As employed some vocabulary in the middle of description and claim to censure special component or method.Art technology
Personnel are it is to be appreciated that same composition may be called with different nouns in different regions.This specification and claims are not
In the way of the difference of title is used as distinguishing composition." comprising " as mentioned by the middle of description and claim in the whole text is
One open language, therefore " comprise but be not limited to " should be construed to." substantially " refer in receivable range of error, this area
Technical staff can solve described technical problem in the range of certain error, basically reaches described technique effect.Description is follow-up
It is described as implementing the better embodiment of the application, for the purpose of right described description is the rule so that the application to be described, not
In order to limit scope of the present application.The protection domain of the application is when being as the criterion depending on the defined person of claims.
Also, it should be noted term " includes ", " comprising " or its any other variant are intended to nonexcludability
Comprise, so that include that the commodity of a series of key element or system not only include those key elements, but also include the most clearly
Other key elements listed, or also include the key element intrinsic for this commodity or system.In the feelings not having more restriction
Under condition, statement " including ... " key element limited, it is not excluded that in the commodity including described key element or system also
There is other identical element.
Described above illustrate and describes some preferred embodiments of invention, but as previously mentioned, it should be understood that invention is not
It is confined to form disclosed herein, is not to be taken as the eliminating to other embodiments, and can be used for other combinations various, amendment
And environment, and can be carried out by above-mentioned teaching or the technology of association area or knowledge in invention contemplated scope described herein
Change.And the change that those skilled in the art are carried out and change are without departing from the spirit and scope of invention, the most all should weigh appended by invention
In the protection domain that profit requires.
Claims (8)
1. the quick breeding method for tissue culture spending Herba Phyllanthi Urinariae in vain, it is characterised in that choose and spend Herba Phyllanthi Urinariae in vain
Blade as group training outer implant；Seedling is formed by blade surface bulbil inducing culture, or by blade wound healing group
Knit inducing culture, adventitious bud induction culture, root culture formation seedling；After seedling grows adventitious root, carry out tissue cultured seedling domestication
Transplant, the most normally cultivate.
2. require the quick breeding method for tissue culture spending Herba Phyllanthi Urinariae in vain as described in 1 such as profit, it is characterised in that specifically include
Following steps:
Step 1, the selection of outer implant and process
Choosing and spending the blade of Herba Phyllanthi Urinariae in vain is parent material, the blade collected is cut into the leaf section of 2～4cm, as group
The outer implant of training；
Step 2, carries out induced synthesis seedling to blade
Outer implant through surface sterilization is inoculated on bulbil inducing culture MS+TDZ2.0mg/L and cultivates at illumination, treat
After blade surface gradually forms complete root system, root system is inserted in culture medium the seedling becoming new；
Or, the outer implant through surface sterilization is inoculated in callus inducing medium MS+2,4D2.0mg/L is carried out dark
Cultivate, treat at paddle cutout, to form white or flaxen callus, be then inoculated in adventitious bud induction culture base MS+
6BA2.0mg/L, is placed at illumination and cultivates, and callus gradually forms adventitious bud, then is transferred to root media MS+
On IBA1.0mg/L, after 20～30d, seedling of taking root becomes new seedling；
Step 3, tissue cultured seedling rooting culture
After the adventitious root that seedling grows 3～5 2～3cm, carry out seedling exercising, then be transplanted in culture matrix enter by tissue cultured seedling
The slow Seedling of row, more normally cultivate.
3. require the quick breeding method for tissue culture spending Herba Phyllanthi Urinariae in vain as described in 2 such as profit, it is characterised in that cultivation temperature
It it is 25 DEG C.
4. require the quick breeding method for tissue culture spending Herba Phyllanthi Urinariae in vain as described in 2 such as profit, it is characterised in that light application time
Being 12～14h/d, intensity of illumination is 40～60u.Mol.m-2.s-1。
5. require the quick breeding method for tissue culture spending Herba Phyllanthi Urinariae in vain as described in 2 such as profit, it is characterised in that bulbil lures
Lead, callus induction, Induce aerosor and root culture, minimal medium used is MS culture medium, and dosage of sucrose is
20～40g/L, coagulator be agar powder, consumption be 6～7g/L, medium pH is 5.8.
6. require the quick breeding method for tissue culture spending Herba Phyllanthi Urinariae in vain as described in 2 such as profit, it is characterised in that described seedling exercising
Particularly as follows: first group training chamber opening bottle cap seedling exercising 3 days, it is then transported to greenhouse or cool canopy, then seedling exercising 3 days.
7. require the quick breeding method for tissue culture spending Herba Phyllanthi Urinariae in vain as described in 2 such as profit, it is characterised in that described cultivation
Substrate is made up of with turfy soil 3:1 in mass ratio sandy loam.
8. the quick breeding method for tissue culture spending Herba Phyllanthi Urinariae in vain as described in profit requires 2 or 7, it is characterised in that described
Within the water content 25% of culture matrix, relative air humidity 80～90%.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106508684A (en) * | 2016-12-08 | 2017-03-22 | 云南省热带作物科学研究所 | Ornithogalum Sauderside isolated culture method |
| CN109430059A (en) * | 2018-12-25 | 2019-03-08 | 云南省农业科学院花卉研究所 | A kind of cut-flower Ornithogalum caudatum in vitro culture quick-breeding method |
| CN111118027A (en) * | 2020-01-17 | 2020-05-08 | 四川天艺优境环境科技有限公司 | Ornithogalum caudatum ait homologous structural domain transcription factor OtPHD1 gene and application thereof |
| CN112243631A (en) * | 2020-09-14 | 2021-01-22 | 云南省农业科学院花卉研究所 | Method for rapidly breaking dormancy of green flower lily seed bulbs |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106508684A (en) * | 2016-12-08 | 2017-03-22 | 云南省热带作物科学研究所 | Ornithogalum Sauderside isolated culture method |
| CN106508684B (en) * | 2016-12-08 | 2018-08-03 | 云南省热带作物科学研究所 | A kind of South Africa Star of Bethlehem in-vitro culture method |
| CN109430059A (en) * | 2018-12-25 | 2019-03-08 | 云南省农业科学院花卉研究所 | A kind of cut-flower Ornithogalum caudatum in vitro culture quick-breeding method |
| CN111118027A (en) * | 2020-01-17 | 2020-05-08 | 四川天艺优境环境科技有限公司 | Ornithogalum caudatum ait homologous structural domain transcription factor OtPHD1 gene and application thereof |
| CN111118027B (en) * | 2020-01-17 | 2022-07-29 | 四川天艺优境环境科技有限公司 | Ornithogalum caudatum ait homologous structure domain transcription factor OtPHD1 gene and application |
| CN112243631A (en) * | 2020-09-14 | 2021-01-22 | 云南省农业科学院花卉研究所 | Method for rapidly breaking dormancy of green flower lily seed bulbs |
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| CN106069756B (en) | 2018-04-06 |
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