CN114376122A - 一种改善大黄鱼肠道结构的饲料添加剂、饲料及应用 - Google Patents
一种改善大黄鱼肠道结构的饲料添加剂、饲料及应用 Download PDFInfo
- Publication number
- CN114376122A CN114376122A CN202210132097.6A CN202210132097A CN114376122A CN 114376122 A CN114376122 A CN 114376122A CN 202210132097 A CN202210132097 A CN 202210132097A CN 114376122 A CN114376122 A CN 114376122A
- Authority
- CN
- China
- Prior art keywords
- feed
- intestinal
- large yellow
- application
- feed additive
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000000968 intestinal effect Effects 0.000 title claims abstract description 27
- 241001596950 Larimichthys crocea Species 0.000 title claims abstract description 22
- 239000003674 animal food additive Substances 0.000 title claims abstract description 15
- ARIWANIATODDMH-UHFFFAOYSA-N rac-1-monolauroylglycerol Chemical compound CCCCCCCCCCCC(=O)OCC(O)CO ARIWANIATODDMH-UHFFFAOYSA-N 0.000 claims abstract description 15
- ARIWANIATODDMH-AWEZNQCLSA-N 1-lauroyl-sn-glycerol Chemical compound CCCCCCCCCCCC(=O)OC[C@@H](O)CO ARIWANIATODDMH-AWEZNQCLSA-N 0.000 claims description 9
- 235000012424 soybean oil Nutrition 0.000 abstract description 15
- 239000003549 soybean oil Substances 0.000 abstract description 15
- 235000021323 fish oil Nutrition 0.000 abstract description 9
- 210000001035 gastrointestinal tract Anatomy 0.000 abstract description 9
- 230000006378 damage Effects 0.000 abstract description 8
- 230000004888 barrier function Effects 0.000 abstract description 5
- 241001465754 Metazoa Species 0.000 abstract description 3
- 230000008859 change Effects 0.000 abstract description 3
- 208000037817 intestinal injury Diseases 0.000 abstract description 3
- 235000016709 nutrition Nutrition 0.000 abstract 1
- 241000251468 Actinopterygii Species 0.000 description 14
- 230000000694 effects Effects 0.000 description 8
- 238000000034 method Methods 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 5
- 230000014509 gene expression Effects 0.000 description 5
- 230000007358 intestinal barrier function Effects 0.000 description 5
- 235000019750 Crude protein Nutrition 0.000 description 4
- WSMYVTOQOOLQHP-UHFFFAOYSA-N Malondialdehyde Chemical compound O=CCC=O WSMYVTOQOOLQHP-UHFFFAOYSA-N 0.000 description 4
- 230000003078 antioxidant effect Effects 0.000 description 4
- 235000019784 crude fat Nutrition 0.000 description 4
- 229940118019 malondialdehyde Drugs 0.000 description 4
- 230000009467 reduction Effects 0.000 description 4
- 235000015112 vegetable and seed oil Nutrition 0.000 description 4
- 239000008158 vegetable oil Substances 0.000 description 4
- 239000003963 antioxidant agent Substances 0.000 description 3
- 238000009360 aquaculture Methods 0.000 description 3
- 244000144974 aquaculture Species 0.000 description 3
- 238000009472 formulation Methods 0.000 description 3
- 210000005027 intestinal barrier Anatomy 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- GHOKWGTUZJEAQD-ZETCQYMHSA-N (D)-(+)-Pantothenic acid Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-ZETCQYMHSA-N 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 235000005911 diet Nutrition 0.000 description 2
- 230000037213 diet Effects 0.000 description 2
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 230000000366 juvenile effect Effects 0.000 description 2
- 239000003921 oil Substances 0.000 description 2
- 235000019198 oils Nutrition 0.000 description 2
- 239000013535 sea water Substances 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- GVJHHUAWPYXKBD-IEOSBIPESA-N α-tocopherol Chemical compound OC1=C(C)C(C)=C2O[C@@](CCC[C@H](C)CCC[C@H](C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-IEOSBIPESA-N 0.000 description 2
- 206010067484 Adverse reaction Diseases 0.000 description 1
- KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 description 1
- 239000004151 Calcium iodate Substances 0.000 description 1
- GHOKWGTUZJEAQD-UHFFFAOYSA-N Chick antidermatitis factor Natural products OCC(C)(C)C(O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-UHFFFAOYSA-N 0.000 description 1
- 241000251464 Coelacanthiformes Species 0.000 description 1
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 1
- 241000239366 Euphausiacea Species 0.000 description 1
- 241001149925 Fenneropenaeus indicus Species 0.000 description 1
- 235000019733 Fish meal Nutrition 0.000 description 1
- 108010068370 Glutens Proteins 0.000 description 1
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 1
- 241000269795 Lateolabrax japonicus Species 0.000 description 1
- 239000005639 Lauric acid Substances 0.000 description 1
- KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 description 1
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 1
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 1
- 235000019482 Palm oil Nutrition 0.000 description 1
- 229930040373 Paraformaldehyde Natural products 0.000 description 1
- 241000276618 Perciformes Species 0.000 description 1
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 1
- 235000019764 Soybean Meal Nutrition 0.000 description 1
- 229930003451 Vitamin B1 Natural products 0.000 description 1
- 229930003779 Vitamin B12 Natural products 0.000 description 1
- 229930003471 Vitamin B2 Natural products 0.000 description 1
- 229930003448 Vitamin K Natural products 0.000 description 1
- 229910021536 Zeolite Inorganic materials 0.000 description 1
- 210000000579 abdominal fat Anatomy 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000006838 adverse reaction Effects 0.000 description 1
- 229940087168 alpha tocopherol Drugs 0.000 description 1
- 235000019728 animal nutrition Nutrition 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229960003237 betaine Drugs 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- UHWJJLGTKIWIJO-UHFFFAOYSA-L calcium iodate Chemical compound [Ca+2].[O-]I(=O)=O.[O-]I(=O)=O UHWJJLGTKIWIJO-UHFFFAOYSA-L 0.000 description 1
- 235000019390 calcium iodate Nutrition 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- GVPFVAHMJGGAJG-UHFFFAOYSA-L cobalt dichloride Chemical compound [Cl-].[Cl-].[Co+2] GVPFVAHMJGGAJG-UHFFFAOYSA-L 0.000 description 1
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 description 1
- 229910000365 copper sulfate Inorganic materials 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 description 1
- 230000006862 enzymatic digestion Effects 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 239000011790 ferrous sulphate Substances 0.000 description 1
- 235000003891 ferrous sulphate Nutrition 0.000 description 1
- 239000004467 fishmeal Substances 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 229960000304 folic acid Drugs 0.000 description 1
- 235000019152 folic acid Nutrition 0.000 description 1
- 239000011724 folic acid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000021312 gluten Nutrition 0.000 description 1
- 125000005456 glyceride group Chemical group 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000036046 immunoreaction Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000003750 lower gastrointestinal tract Anatomy 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 229940099596 manganese sulfate Drugs 0.000 description 1
- 239000011702 manganese sulphate Substances 0.000 description 1
- 235000007079 manganese sulphate Nutrition 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 238000009364 mariculture Methods 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- POULHZVOKOAJMA-UHFFFAOYSA-N methyl undecanoic acid Natural products CCCCCCCCCCCC(O)=O POULHZVOKOAJMA-UHFFFAOYSA-N 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 230000003387 muscular Effects 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000004792 oxidative damage Effects 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000002540 palm oil Substances 0.000 description 1
- 229940055726 pantothenic acid Drugs 0.000 description 1
- 235000019161 pantothenic acid Nutrition 0.000 description 1
- 239000011713 pantothenic acid Substances 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 229920002866 paraformaldehyde Polymers 0.000 description 1
- SHUZOJHMOBOZST-UHFFFAOYSA-N phylloquinone Natural products CC(C)CCCCC(C)CCC(C)CCCC(=CCC1=C(C)C(=O)c2ccccc2C1=O)C SHUZOJHMOBOZST-UHFFFAOYSA-N 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- ZUFQODAHGAHPFQ-UHFFFAOYSA-N pyridoxine hydrochloride Chemical compound Cl.CC1=NC=C(CO)C(CO)=C1O ZUFQODAHGAHPFQ-UHFFFAOYSA-N 0.000 description 1
- 229960004172 pyridoxine hydrochloride Drugs 0.000 description 1
- 235000019171 pyridoxine hydrochloride Nutrition 0.000 description 1
- 239000011764 pyridoxine hydrochloride Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229960000342 retinol acetate Drugs 0.000 description 1
- 235000019173 retinyl acetate Nutrition 0.000 description 1
- 239000011770 retinyl acetate Substances 0.000 description 1
- QGNJRVVDBSJHIZ-QHLGVNSISA-N retinyl acetate Chemical compound CC(=O)OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C QGNJRVVDBSJHIZ-QHLGVNSISA-N 0.000 description 1
- 229960002477 riboflavin Drugs 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 239000011781 sodium selenite Substances 0.000 description 1
- 235000015921 sodium selenite Nutrition 0.000 description 1
- 229960001471 sodium selenite Drugs 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000004455 soybean meal Substances 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 229960003495 thiamine Drugs 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 229960000984 tocofersolan Drugs 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 235000010374 vitamin B1 Nutrition 0.000 description 1
- 239000011691 vitamin B1 Substances 0.000 description 1
- 235000019163 vitamin B12 Nutrition 0.000 description 1
- 239000011715 vitamin B12 Substances 0.000 description 1
- 235000019164 vitamin B2 Nutrition 0.000 description 1
- 239000011716 vitamin B2 Substances 0.000 description 1
- 235000019168 vitamin K Nutrition 0.000 description 1
- 239000011712 vitamin K Substances 0.000 description 1
- 150000003721 vitamin K derivatives Chemical class 0.000 description 1
- 229940046010 vitamin k Drugs 0.000 description 1
- 239000010457 zeolite Substances 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- 229960001763 zinc sulfate Drugs 0.000 description 1
- 235000004835 α-tocopherol Nutrition 0.000 description 1
- 239000002076 α-tocopherol Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Birds (AREA)
- Insects & Arthropods (AREA)
- Marine Sciences & Fisheries (AREA)
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
Abstract
本发明涉及一种改善大黄鱼肠道结构的饲料添加剂、饲料及应用，水产动物营养饲料领域，所述的饲料添加剂中包括单月桂酸甘油酯。本发明还提供一种包含所述饲料添加剂的饲料，在所述饲料中单月桂酸甘油酯的质量百分比为0.04％。本发明还提供所述饲料添加剂在改善大黄鱼肠道结构中的应用，所述应用时间为70天以上。本发明饲料能够有效改善大豆油替代鱼油引发的肠道形态改变，肠绒毛减少，肠道周长比降低，肠道屏障损伤；缓解大豆油替代鱼油引发的肠道抗氧化力损伤。
Description
技术领域
本发明属于水产动物营养饲料领域，具体地涉及一种改善大黄鱼(Larimichthyscrocea)肠道结构的饲料添加剂、饲料及应用。
背景技术
大黄鱼属于硬骨鱼纲的鲈形目、石首鱼科、黄鱼属，主要分布在我国东南沿海海域，其肉质鲜美，且具有一定的药用价值，是我国特有的海水经济鱼类。根据2021年中国渔业统计年鉴显示，大黄鱼的养殖产量超过25万吨，是我国养殖产量最大的海水经济鱼类。
随着近年来我国水产养殖业的快速发展，对鱼类饲料优质脂肪源-鱼油的需求也在不断扩大，鱼油的供给与需求关系逐渐失衡，寻找鱼油的替代物已成为海水鱼养殖饲料行业的热点。由于大豆油、棕榈油等植物油具有价格低、来源广和脂肪酸种类含量丰富的特点，被广泛应用于大黄鱼等海水鱼的养殖中。但生产实际发现高比例的植物油添加往往易导致鱼类肠道屏障受损、肠绒毛减少、肠道结构改变、抗氧化能力损伤等不良反应，损害了鱼体健康，也制约着集约化海水养殖的发展。因此寻找特定的调控手段解决高比例植物油替代引发肠道损伤问题刻不容缓。
单月桂酸甘油酯是一种月桂酸的单甘油衍生物，因其具有良好的抗菌、抗病毒能力和一定的促生长功能，在水产动物养殖中具有较广泛的应用。研究发现，在花鲈的养殖饲料中添加一定比例的单月桂酸甘油酯，可以降低其腹部脂肪沉积；在白对虾的养殖中使用单月桂酸甘油酯可以改善消化酶活和非特异性免疫反应，促进生长。然而尚不清楚单月桂酸甘油酯是否具有对水产动物的肠道结构修复和肠道损伤的缓解作用。
发明内容
本发明要解决的问题在于提供一种改善大黄鱼肠道结构的饲料添加剂、饲料及应用，通过添加其在饲料中特定比例的单月桂酸甘油酯来改变大黄鱼肠道屏障损伤、肠绒毛减少和抗氧化力下降等问题，以解决水产养殖业油源替代过程中的技术壁垒。
本发明是通过如下技术方案来实现的：
一种改善大黄鱼肠道结构的饲料添加剂，所述的饲料添加剂中包括单月桂酸甘油酯。
本发明还提供一种包含所述饲料添加剂的饲料，在所述饲料中单月桂酸甘油酯的质量百分比为0.04％。
本发明还提供所述饲料在改善大黄鱼肠道结构中的应用，所述应用时间为70天以上。
本发明与现有技术相比的有益效果：
1)本发明所述饲料添加剂和饲料能够有效改善大豆油替代鱼油引发的肠道形态改变，肠绒毛减少，肠道屏障损伤。
2)本发明所述饲料添加剂和饲料能够有效缓解大豆油替代鱼油引发的肠道抗氧化力损伤(抗氧化酶活力提高，丙二醛含量下降)。
3)本发明所述单月桂酸甘油酯为鱼类所需营养物质，无任何毒副作用，对食品安全无任何影响。
附图说明
为了更清楚地说明本发明具体实施方式或现有技术中的技术方案，下面将对具体实施方式或现有技术描述中所需要使用的附图作简单地介绍，显而易见地,下面描述中的附图是本发明的一些实施方式，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下，还可以根据这些附图获得其他的附图。
图1、本发明实施例1中各组间大黄鱼肠道形态H&E染色结果；a鱼油组，b豆油组，c豆油组中添加质量比为0.02％单月桂酸甘油酯，d、豆油组中添加质量比为0.04％单月桂酸甘油酯，e、豆油组中添加质量比为0.08％单月桂酸甘油酯；
图2、本发明实施例1中各组间大黄鱼肠道绒毛高度、肌层厚度和周长比；
图3、本发明实施例1中各组间大黄鱼肠道物理屏障相关基因表达；
图4、本发明实施例1中各组间大黄鱼肠道抗氧化酶活力及丙二醛含量。
具体实施方式
下面通过实施例对本发明的技术特征作进一步解释，但本发明的保护范围不受实施例任何形式上的限制。
实施例1、使用本方法进行饲料制造及养殖管理
1、实验设计和实验饲料配方
以本实验室往年所用大黄鱼养殖大豆油替代饲料配方为基础配方，在基础配方中添加含有不同质量比的单月桂酸甘油酯的饲料添加剂，使饲料中单月桂酸甘油酯的质量比分别为0.02％、0.04％和0.08％(即200mg/kg、400mg/kg和800mg/kg)。另外，以常规鱼油饲料组作为阴性对照组。饲料配方见表1。
表1实验饲料的配方和粗成分(％干成分)
1白鱼粉(74％粗蛋白，12.6％粗脂肪)；高级磷虾粉(53.18％粗蛋白，13％粗脂肪)；去皮豆粕(46.68％粗蛋白，0.33％粗脂肪)；高筋面粉(19.66％粗蛋白，0.98粗脂肪)。
2多维预混料(mg/kg diet)(维生素A醋酸酯,3；维生素,D3 5；α-生育酚,240；维生素K,240；维生素B1,25；维生素B2,45；盐酸吡哆醇,20；维生素B12,10；泛酸,60；叶酸,20；烟酸,200；生物素,60；肌醇,800；维晶纤维素,13473)
3多矿预混料(mg/kg diet)(硫酸镁,1200；硫酸铜,10；硫酸亚铁,80，硫酸锌,50；硫酸锰,45；氯化钴,50；亚硒酸钠,20；碘酸钙,60；沸石粉,13485；)
4甘氨酸和甜菜碱。
5单月桂酸甘油酯：纯度92％。
2、实验用鱼和养殖管理
本实验选用初始体重为13g的实验大黄鱼幼鱼共540尾，鱼苗购自福建省宁德市富发水产有限公司。随机分成三个组，每个组三个重复，每个重复60尾鱼，养殖周期为10周。正式实验开始后，每天早上5：00和晚上17：00进行饱食投喂，不定期清理水面杂物、打捞死鱼。实验期间水温维持在19.3-22.8℃，盐度在25.6-29.9‰，溶氧水平在6.1-7.0mg/L之间。
3、实验样品采集与分析
养殖实验结束后，在经过24小时的禁食以后进行取样。实验鱼麻醉后，迅速解剖取出肠道放入液氮储存，用于后续分析肠道屏障相关基因表达、抗氧化酶活力。再另外取3尾幼鱼获得后肠，经PBS漂洗后放入多聚甲醛中固定用于制作石蜡切片。肠道抗氧化酶活力及丙二醛含量通过商业试剂盒进行检测。
如图1显示，豆油组与鱼油组相比，呈现出明显的肠道结构损伤，而使用本方法的添加组的肠道结构损伤被缓解，肠道绒毛数量增多；
如图2显示，豆油组的肠道绒毛高度和周长比明显降低，而使用本方法的添加组肠道绒毛高度相对改善；其中添加量为0.04％组肠道周长比明显高于豆油组。
如图3显示，豆油组的肠道屏障功能相关基因表达量显著降低，而使用本方法的添加组的肠道屏障相关基因表达量相对提高；其中添加量为0.04％组的肠道屏障相关基因表达量明显高于豆油组。
如图4显示，豆油组的肠道抗氧化酶活力显著下降，丙二醛含量下降，而使用本方法的添加组抗氧化力明显改善。其中添加量为0.04％组的抗氧化力明显高于豆油组。
Claims (3)
1.一种改善大黄鱼肠道结构的饲料添加剂，其特征在于所述的饲料添加剂中包括单月桂酸甘油酯。
2.一种包含权利要求1所述饲料添加剂的饲料，其特征在于所述饲料中单月桂酸甘油酯的质量百分比为0.04％。
3.权利要求2所述饲料在改善大黄鱼肠道结构中的应用，所述应用时间为70天以上。
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210132097.6A CN114376122A (zh) | 2022-02-14 | 2022-02-14 | 一种改善大黄鱼肠道结构的饲料添加剂、饲料及应用 |
| US18/109,523 US20230255239A1 (en) | 2022-02-14 | 2023-02-14 | Feed additive for improving intestinal structure of larimichthys crocea, feed, and use |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210132097.6A CN114376122A (zh) | 2022-02-14 | 2022-02-14 | 一种改善大黄鱼肠道结构的饲料添加剂、饲料及应用 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN114376122A true CN114376122A (zh) | 2022-04-22 |
Family
ID=81206087
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210132097.6A Pending CN114376122A (zh) | 2022-02-14 | 2022-02-14 | 一种改善大黄鱼肠道结构的饲料添加剂、饲料及应用 |
Country Status (2)
| Country | Link |
|---|---|
| US (1) | US20230255239A1 (zh) |
| CN (1) | CN114376122A (zh) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105410365A (zh) * | 2015-12-17 | 2016-03-23 | 浙江大学 | 一种可替代抗生素的饲料添加剂及其应用 |
| WO2016121007A1 (ja) * | 2015-01-27 | 2016-08-04 | 株式会社トーワ | 腸管改善用飼料添加剤及び飼料 |
| CN107028028A (zh) * | 2017-05-12 | 2017-08-11 | 黄平野洞河袁氏生态循环种养殖有限责任公司 | 一种泥鳅膨化饲料及其制备方法 |
| CN108967666A (zh) * | 2018-10-09 | 2018-12-11 | 湖北浩华生物技术有限公司 | 一种抗菌饲料添加剂及其用途 |
| CN110226673A (zh) * | 2019-07-08 | 2019-09-13 | 中国水产科学研究院淡水渔业研究中心 | 一种加州鲈鱼养殖中保肝健肠的复合制剂及其制备方法 |
| CN112042810A (zh) * | 2020-09-03 | 2020-12-08 | 浙江大学 | 复合饲料添加剂在制备提高养殖大黄鱼肌肉质地的饲料中的应用 |
| US20210244048A1 (en) * | 2020-02-11 | 2021-08-12 | Hangzhou Longyu Bio-Technology Co., Ltd. | Feed additive and application thereof |
| CN113439801A (zh) * | 2021-06-21 | 2021-09-28 | 山东得和明兴生物科技有限公司 | 一种替抗产品及其制备方法 |
| CN113768047A (zh) * | 2021-09-14 | 2021-12-10 | 浙江大学 | 一种缓释型丁酸月桂酸混合结构酯及其制备方法和应用 |
-
2022
- 2022-02-14 CN CN202210132097.6A patent/CN114376122A/zh active Pending
-
2023
- 2023-02-14 US US18/109,523 patent/US20230255239A1/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016121007A1 (ja) * | 2015-01-27 | 2016-08-04 | 株式会社トーワ | 腸管改善用飼料添加剤及び飼料 |
| CN105410365A (zh) * | 2015-12-17 | 2016-03-23 | 浙江大学 | 一种可替代抗生素的饲料添加剂及其应用 |
| CN107028028A (zh) * | 2017-05-12 | 2017-08-11 | 黄平野洞河袁氏生态循环种养殖有限责任公司 | 一种泥鳅膨化饲料及其制备方法 |
| CN108967666A (zh) * | 2018-10-09 | 2018-12-11 | 湖北浩华生物技术有限公司 | 一种抗菌饲料添加剂及其用途 |
| CN110226673A (zh) * | 2019-07-08 | 2019-09-13 | 中国水产科学研究院淡水渔业研究中心 | 一种加州鲈鱼养殖中保肝健肠的复合制剂及其制备方法 |
| US20210244048A1 (en) * | 2020-02-11 | 2021-08-12 | Hangzhou Longyu Bio-Technology Co., Ltd. | Feed additive and application thereof |
| CN112042810A (zh) * | 2020-09-03 | 2020-12-08 | 浙江大学 | 复合饲料添加剂在制备提高养殖大黄鱼肌肉质地的饲料中的应用 |
| CN113439801A (zh) * | 2021-06-21 | 2021-09-28 | 山东得和明兴生物科技有限公司 | 一种替抗产品及其制备方法 |
| CN113768047A (zh) * | 2021-09-14 | 2021-12-10 | 浙江大学 | 一种缓释型丁酸月桂酸混合结构酯及其制备方法和应用 |
Non-Patent Citations (2)
| Title |
|---|
| QIUFEN MO 等: ""High-Dose Glycerol Monolaurate Up-Regulated Beneficial Indigenous Microbiota without Inducing Metabolic Dysfunction and Systemic Inflammation: New Insights into Its Antimicrobial Potential"", 《NUTRIENTS》 * |
| 刘梦芸等: "饲粮中添加单月桂酸甘油酯对肉鸡生长性能、消化能力及鸡肉营养品质的影响", 《食品科学》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| US20230255239A1 (en) | 2023-08-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Ding | Grass carp, Ctenopharyngodon idella | |
| Reis et al. | Protein-to-energy ratios in production diets and growth, feed conversion and body composition of channel catfish, Ictalurus punctatus | |
| CN105614159A (zh) | 一种本地乌鳢全程使用的膨化配合饲料 | |
| CN102860427A (zh) | 一种添加浒苔制备刺参膨化饲料的方法 | |
| CN112293570B (zh) | 一种适用于低盐度养殖条件的凡纳滨对虾低鱼粉配合饲料 | |
| CN105533291B (zh) | 一种无鱼粉无豆粕黄河鲤鱼配合饲料及其制备方法 | |
| CN110651896A (zh) | 一种蛙类配合饲料 | |
| KR100503966B1 (ko) | 돌돔 양식용 사료 조성물 | |
| CN114376122A (zh) | 一种改善大黄鱼肠道结构的饲料添加剂、饲料及应用 | |
| CN107319208B (zh) | 一种水产饲料及其在石斑鱼饲养中的应用 | |
| CN113925115B (zh) | 一种大黄鱼幼鱼低水温养殖复合诱食剂及其应用 | |
| CN112262795B (zh) | 一种维持低脂型养殖鱼类肌肉脂肪酸品质的营养学方法 | |
| CN109907181A (zh) | 一种低鱼粉蛋白珍珠龙胆石斑鱼配合饲料 | |
| CN107897577A (zh) | 青虾双壳症防控饲料及防控方法 | |
| CN110476844B (zh) | 一种提高促进鱼体肌纤维发育和dha沉积的方法 | |
| Ramezani-Fard et al. | Changes over time in muscle fatty acid composition of Malaysian mahseer, Tor tambroides, fed different dietary lipid percentage | |
| CN114376116A (zh) | 黄颡鱼低锰饲料及其制备方法 | |
| CN111184148A (zh) | 一种促进黄颡鱼生长及提高利用率的饲料配方及加工工艺 | |
| CN104970230A (zh) | 一种大菱鲆低鱼粉配合饲料 | |
| CN115606715B (zh) | 一种含诱食剂的淡水鱼饲料及其制备方法 | |
| Venkateswarlu | Growth performance effect of dietary fish meal quality in shrimp Litopenaeus vannamei | |
| CN108902544A (zh) | 用于中华绒螯蟹育肥的饲料 | |
| CN114009633A (zh) | 一种提高浅色黄姑鱼鱼鳔品质与肌肉品质的饲料预混料 | |
| CN117814367A (zh) | 一种花鲈饲料添加剂及其应用 | |
| CN118216627A (zh) | 一种保障肝肠健康的低鱼粉斑节对虾配合饲料及其应用 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220422 |