CN114040979B - 稳定地保持外源基因的人工重组rna病毒的制作方法 - Google Patents

稳定地保持外源基因的人工重组rna病毒的制作方法 Download PDF

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CN114040979B
CN114040979B CN202080045560.2A CN202080045560A CN114040979B CN 114040979 B CN114040979 B CN 114040979B CN 202080045560 A CN202080045560 A CN 202080045560A CN 114040979 B CN114040979 B CN 114040979B
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rotavirus
artificial recombinant
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CN114040979A (zh
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小林刚
金井祐太
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University of Osaka NUC
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C12N15/867—Retroviral vectors
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    • C12N2720/12311—Rotavirus, e.g. rotavirus A
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    • C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
    • C12N2720/00011—Details
    • C12N2720/12011—Reoviridae
    • C12N2720/12311—Rotavirus, e.g. rotavirus A
    • C12N2720/12322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
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    • C12N2720/00011—Details
    • C12N2720/12011—Reoviridae
    • C12N2720/12311—Rotavirus, e.g. rotavirus A
    • C12N2720/12341—Use of virus, viral particle or viral elements as a vector
    • C12N2720/12343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host

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CN202080045560.2A 2019-06-21 2020-06-19 稳定地保持外源基因的人工重组rna病毒的制作方法 Active CN114040979B (zh)

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JP2019115532 2019-06-21
JP2019-115532 2019-06-21
PCT/JP2020/024131 WO2020256099A1 (ja) 2019-06-21 2020-06-19 外来遺伝子を安定的に保持する人工組換えｒｎａウイルスの作製方法

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CN114040979A CN114040979A (zh) 2022-02-11
CN114040979B true CN114040979B (zh) 2024-06-28

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US (1) US12305192B2 (https=)
EP (1) EP3988650A4 (https=)
JP (1) JP7517704B2 (https=)
CN (1) CN114040979B (https=)
WO (1) WO2020256099A1 (https=)

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102666860A (zh) * 2009-07-31 2012-09-12 诺华有限公司 反向遗传系统

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IL140441A0 (en) * 1998-09-29 2002-02-10 Maxygen Inc Shuffling of codon altered genes
CN1934260B (zh) * 2004-01-22 2013-03-13 株式会社载体研究所 利用含有巨细胞病毒增强子和鸡β-肌动蛋白启动子的杂合启动子制造负链RNA病毒载体的方法
ATE453716T1 (de) 2004-08-03 2010-01-15 Geneart Ag Verfahren zur modulation der genexpression durch änderung des cpg gehalts
DE102004037611B4 (de) 2004-08-03 2013-10-02 Geneart Ag Induzierbare Genexpression
JP2009519715A (ja) * 2005-12-16 2009-05-21 ザ ユーエービー リサーチ ファウンデーション ウイルスベクターを使用する制御された遺伝子発現に関する組成物および方法
CN101245350B (zh) * 2007-02-17 2011-12-28 王健伟 密码子优化的轮状病毒蛋白的编码核苷酸序列、其重组体及其应用
KR101621100B1 (ko) * 2007-03-30 2016-05-13 더 리서치 파운데이션 오브 스테이트 유니버시티 오브 뉴욕 백신에 유용한 약독화 바이러스
GB0800272D0 (en) * 2008-01-08 2008-02-13 Plant Bioscience Ltd Protein expression systems
CN101502650B (zh) * 2009-03-10 2011-12-28 邢益平 密码子优化的乙型肝炎核酸疫苗
TWI696700B (zh) * 2015-01-16 2020-06-21 國立研究開發法人產業技術總合研究所 使用具有匿跡性之ｒｎａ的基因表現系統及含有該ｒｎａ的基因導入、表現載體
CN104830874B (zh) * 2015-04-16 2017-11-21 南京医科大学第一附属医院 密码子优化的严重发热伴血小板减少综合征病毒核蛋白基因及其核酸疫苗
JP7054143B2 (ja) * 2016-05-11 2022-04-13 国立大学法人大阪大学 キメラ抗原受容体、及びその利用
EP4299766A3 (en) 2016-09-27 2024-05-01 Osaka University Method for preparing artificial recombinant rotavirus
CN110198733B (zh) 2016-09-28 2024-04-30 巴法里安诺迪克有限公司 用于增强痘病毒中转基因的稳定性的组合物和方法

Patent Citations (1)

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CN102666860A (zh) * 2009-07-31 2012-09-12 诺华有限公司 反向遗传系统

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JPWO2020256099A1 (https=) 2020-12-24
CN114040979A (zh) 2022-02-11
US20220356491A1 (en) 2022-11-10
EP3988650A1 (en) 2022-04-27
EP3988650A4 (en) 2022-09-21
US12305192B2 (en) 2025-05-20
JP7517704B2 (ja) 2024-07-17
WO2020256099A1 (ja) 2020-12-24

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