CN113430174A - 一株高裂解性沙门氏菌噬菌体rdp-sa-19009及其应用 - Google Patents

一株高裂解性沙门氏菌噬菌体rdp-sa-19009及其应用 Download PDF

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CN113430174A
CN113430174A CN202110539840.5A CN202110539840A CN113430174A CN 113430174 A CN113430174 A CN 113430174A CN 202110539840 A CN202110539840 A CN 202110539840A CN 113430174 A CN113430174 A CN 113430174A
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salmonella
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杜新永
王晓铃
于瀚学
李中满
李先胜
马如霞
刘玉庆
张庆
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Abstract

本发明公开了一株高裂解性沙门氏菌噬菌体RDP‑SA‑19009，保藏编号为CGMCCNo.21993，基因长度87591bp，对全基因进行测序后发现，不具有毒力基因和溶原性基因；噬菌体在80℃高温条件下作用30min，效价保持在1.0×106pfu/mL以上，对温度有较高的耐受性；本发明提供的沙门氏菌噬菌体RDP‑SA‑19009可以裂解包括鼠伤寒沙门氏菌、斯坦利沙门氏菌、肠炎沙门氏菌、肯塔基沙门氏菌、伤寒沙门氏菌、山夫登堡沙门氏菌等在内的致病菌，具有裂解谱广、裂解率高的特点，该噬菌体既可以单独使用，也可以和其他沙门氏菌的噬菌体和益生菌复配使用，可以有效治疗和预防降低禽类沙门氏菌病。

Description

一株高裂解性沙门氏菌噬菌体RDP-SA-19009及其应用
技术领域
本发明涉及生物技术领域，特别是涉及一株宽裂解普沙门氏菌噬菌体RDP-SA-19009及其在禽类动物致病性沙门氏菌感染中的应用。
背景技术
沙门氏菌病是公共卫生学上具有重要意义的人畜共患病之一,是一种常见的食源性致病菌。鸡是沙门氏菌最重要的宿主，沙门氏菌可引起鸡急性或慢性疾病，感染沙门氏菌的鸡也可通过产出带菌的蛋导致沙门菌的垂直传播，从而形成恶性循环。当鸡群暴发沙门氏菌病时死亡率可达80％，对养禽业造成巨大的经济损失。人一旦摄入了含有大量沙门氏菌的动物性食品，可导致食源性中毒，据统计在世界各国的种类细菌性食物中毒中，沙门氏菌引起的食物中毒常列榜首。抗生素一直以来被作为治疗细菌感染的首选而得到广泛应用。但随着抗生素的广泛使用甚至滥用，目前细菌对抗生素的耐药性问题已十分严重，抗生素耐药性正在对全球健康构成威胁。
噬菌体(Bacteriophage或Phage)是自然界中最丰富的生物之一，在地球上大约蕴藏着1031的噬菌体颗粒，也就是平均每1g土壤或水中包含了多达108数量的噬菌体。长期以来，其一直被认为可作为治疗药物和杀菌剂使用。噬菌体对某些细菌菌株具有高度特异性，其取决于噬菌体吸附器官和受体菌表面受体的分子结构和互补性，不会影响生物体或内源性组织的正常运转。另外，即使随着细菌耐药性的发展，噬菌体仍会大量保持。在过去几年中，使用噬菌体来检测和控制食品和环境中致病菌及腐败菌的生长受到越来越多研究者的关注，并且该方法已有许多研究支持。Bmvko LYu等讨论了噬菌体检测致病菌的优缺点，指出利用噬菌体检测食品安全及加工制造过程等方面存在的致病菌具有极大的应用前景。例如在蜜瓜切片、鸡胸肉、白菜、牛奶、鸡皮、豆芽菜等食品中发现使用噬菌体制剂后沙门氏菌菌量显著减少。另外，已从多种食物如碎牛肉、猪肉等肉制品、奶制品、生菜等分离出沙门氏菌噬菌体，因此，噬菌体是食品中的天然微生物。本试验从现代化肉鸡养殖场采集粪便、污水和肝脏等样品，筛选能裂解多株病原菌的宽噬菌谱沙门氏菌烈性噬菌体，并对其生物学特性进行分析，以期为噬菌体防治沙门氏菌的研究提供理论依据。
发明内容
本发明的目的在于提供一株宽裂解普沙门氏菌噬菌体RDP-SA-19009，旨在解决养殖场禽类因感染沙门氏菌，发病急而治疗不及时，且因致病菌耐药性而导致用药无效等问题。
为实现上述目的，本发明采用如下技术方案：
一株高裂解性沙门氏菌噬菌体RDP-SA-19009，透过电镜观察，该噬菌体头部为廿面体，且立体对称，头部长54.754nm，尾部长度为178.143nm，为有尾噬菌体目长尾噬菌体科，基因全长87591bp，不具有毒力基因和溶原基因，该噬菌体于2021年03月25日保藏于中国微生物菌种保藏管理委员会普通微生物中心，保藏地址为：北京市朝阳区北辰西路1号院3号，保藏编号CGMCC No.21993。
进一步地，该噬菌体在80℃高温条件下作用30min，效价保持在1.0×106pfu/mL以上，具有较高的生物活性，对温度有较好的耐受性。
进一步地，该噬菌体RDP-SA-19009可高效感染宿主菌，其最佳感染复数为0.001。
进一步地，该噬菌体RDP-SA-19009在体外抑制其宿主菌BS-19009长达12h。
本发明还提供了沙门氏菌噬菌体RDP-SA-19009在制备预防和或治疗沙门氏菌引起的疾病的药物中的用途。
本发明还提供了沙门氏菌噬菌体RDP-SA-19009在制备预防和或治疗沙门氏菌引起的疾病的饲料中的用途。
本发明还提供了包括有效量的沙门氏菌噬菌体RDP-SA-19009。
进一步地，还包括用于抗沙门氏菌的抗生素。
本发明还提供了一种预防和或治疗沙门氏菌引起的疾病的添加剂，其有效成分包括沙门氏菌噬菌体RDP-SA-19009。
一种清洁剂或消毒剂，包括沙门氏菌噬菌体RDP-SA-19009。
本发明的有益效果体现在：
(1)本发明从青岛即墨一具有沙门氏菌细菌性疾病的鸡场的粪便中取样分离得到沙门氏菌BS-19009，并通过实验确定了BS-19009的致病性和耐药性，以BS-19009为宿主，从环境中分离得到一株高裂解性沙门氏菌噬菌体，该噬菌体RDP-SA-19009对禽类养殖环境中致病性沙门氏菌BS-19009具有强裂解效用，为工业化生产噬菌体用于禽类养殖环境中致病性沙门氏菌BS-19009的防治提供了噬菌体来源；
(2)本发明所提供的沙门氏菌噬菌体RDP-SA-19009的裂解性高，裂解谱广，能裂解包括鼠伤寒沙门氏菌、斯坦利沙门氏菌、肠炎沙门氏菌、肯塔基沙门氏菌、甲型副伤寒沙门氏菌、乙型副伤寒沙门氏菌、伤寒沙门氏菌、山夫登堡沙门氏菌在内的致病菌，并具有裂解率高的特点，能裂解具有耐药性的沙门氏菌；在治疗沙门氏菌感染疾病具有很好的优势，效价较高，防治效果好。
(3)本发明所提供的沙门氏菌噬菌体RDP-SA-19009在防控禽类致病性沙门氏菌中具有良好的应用前景。
附图说明
本发明上述的以及其他的特征、性质和优势将通过下面结合附图和实施例的描述而变的更加明显：
图1为本发明噬菌体RDP-SA-19009的噬菌斑示意图；
图2为本发明噬菌体RDP-SA-19009的电镜图；
图3为本发明噬菌体RDP-SA-19009的RTD实验图；
图4为本发明噬菌体RDP-SA-19009的体外抑菌实验图；
图5为本发明噬菌体RDP-SA-19009在4℃下对宿主菌的裂解图；
图6为本发明噬菌体RDP-SA-19009在37℃下对宿主菌的裂解图；
具体实施方式
下面结合附图和实施例对本发明作进一步说明。以下实施例将有助于本领域的技术人员进一步理解本发明，但不以任何形式限制本发明。应当指出的是，对本领域的普通技术人员来说，在不脱离本发明构思的前提下，还可以做出若干变形和改进。这些都属于本发明的保护范围。
本发明使用从青岛即墨肉鸡养殖场粪便中分离得到的沙门氏菌BS-19009，并通过实验确定了BS-19009的致病性和耐药性，以BS-19009为宿主，从环境中分离得到一株沙门氏菌噬菌体RDP-SA-19009，并通过全基因组序列确定了RDP-SA-19009的基因型。
实施例1致病性沙门氏菌BS-19009的分离及其鉴定
从具有沙门氏菌细菌性疾病的鸡场的粪便中取样，采用无菌操作的方法，接种在SS培养基中，37℃条件下培养12-14h。
选择黑色菌落连续划线纯化三次，然后挑取单菌落接种于5ml的LB肉汤液体培养基中，37℃，200rpm振荡培养8h，得到均匀浑浊的细菌悬浮液。再通过16sRNA分子鉴定，确定为沙门氏菌，将其中一株命名为BS-19009；并通过实验确定了BS-19009的致病性和耐药性。
实施例2沙门氏菌噬菌体RDP-SA-19009的分离鉴定
(1)噬菌体RDP-SA-19009的分离：
取粪便10㎎置于10ml LB肉汤中4℃浸泡过夜，10000rpm离心5min，取上清液用0.22μm微孔滤膜过滤除菌。取3ml滤液和3ml处于对数生长期的宿主菌培养液，一起加入到20ml高压灭菌处理的LB肉汤中，静置于37℃培养箱中，过夜培养，对可能存在的噬菌体进行增殖。取样品培养液3ml，10000rpm离心5min,取上清液用0.22μm微孔滤膜过滤除菌。假设上述滤液中含有噬菌体。用LB肉汤进行梯度稀释，与处于对数生长期的宿主增殖液制备双层平板，在37摄氏度下培养19-23h，直到双层平板上出现透明的噬菌斑。
(2)噬菌体的纯化
从有明显单个噬菌斑的双平板中挑取噬菌斑透亮、圆滑、小的噬菌斑，置于生理盐水中，用移液枪经过反复吹打，用0.22μm微孔滤膜过滤除菌，用LB肉汤进行梯度稀释，选择可以得到单个噬菌斑的梯度制备双层平板。以上步骤重复4-5次，直到得到的双平板上的噬菌斑大小、形态和清晰度相同时，噬菌体纯化完成。结果如图1所示，其噬菌斑形态、大小和清晰度一致，为典型的裂解性噬菌体。
(3)噬菌体效价的测定
挑取已纯化完成的噬菌体的单个噬菌斑，加入到LB肉汤中，同时加入处于对数生长期的宿主菌培养液，37℃、170rpm振荡培养6h。取噬菌体增殖液5ml 10000rpm离心5min，取上清液用0.22μm微孔滤膜过滤除菌。用LB肉汤做10倍梯度稀释至109倍，取107、108、109倍稀释度的稀释液0.1ml和宿主菌增殖液0.1ml制备双层平板，测定噬菌体的效价。
其中，噬菌体效价(pfu/mL)＝噬菌斑数×稀释倍数×10；经测定该噬菌体的效价可达1010pfu/mL。该噬菌体最适温度为37℃，效价能达到1010pfu/mL，该噬菌体在-80℃的条件下可长期保存，效价维持稳定。
实施例3噬菌体的形态观察
运用磷钨酸负染法：将噬菌体增殖液20μL(效价1010pfu/mL)滴在石腊片上，将铜网放在上面10min后取下，以吸附噬菌体。在自然室温下干燥2-3min，用2％磷钨酸水液染色，2min后吸干水分，自然干燥5min，用电镜观察噬菌体形态。由电镜照片(图2)可看出，噬菌体RDP-SA-19009头部为廿面体，且立体对称，头部长54.754nm，噬菌体尾部长度为178.143nm，根据噬菌体最新分类与命名，属于有尾噬菌体目长尾噬菌体科。
实施例4菌体基因组测序
通过对单株噬菌体的富集培养后，在4℃条件下8000g离心15分钟，加入10％PEG8000和0.5M NaCl静置过夜，再加入等量氯仿混匀，静置分层后5000g下离心10min，去处氯仿层和PEG层后，加入限制性核酸内切酶消化处理，并采用梯度密度氯化铯条件下悬浮噬菌体，后期采用TM缓冲液透析3次，每次30min，一部分送华大基因生物测序公司进行全基因组测序。该噬菌体基因组全长为87591bp，不具有毒力基因和溶原基因。
实施例5最佳感染复数(MOI)实验
MOI指初始感染时噬菌体数量与宿主菌数量的比值。将噬菌体增殖液0.5ml(效价1010pfu/mL)用LB肉汤进行10倍梯度稀释，按照MOI值为1000、100、10、1、0.1、0.01、0.001，分别取每个梯度0.1ml稀释液和0.1ml的宿主菌增殖液(106fu/mL)加至5ml的LB肉汤中，37℃、200rpm振荡培养8h。将培养液10000rpm离心5min，采用双层平板法测其效价，效价最高的组合为其最佳感染复数。结果如表1所示。
表1最佳感染复数测定
Figure BDA0003071235400000061
由表1可知，该沙门氏菌噬菌体RDP-SA-19009的最佳感染复数是0.001。
实施例6 RTD实验
将噬菌体增殖液0.5ml(效价1010pfu/mL)用LB肉汤进行10倍梯度稀释，先滴加0.01ml宿主菌增殖液(效价106pfu/mL)，待其干后，再滴加0.01ml噬菌体稀释液，37℃下培养14-16h；结果如图3所示。
由图3可知，噬菌体RDP-SA-19009出现完全融合性裂解所需要的噬菌体稀释度为-3。
实施例7体外抑菌实验
取噬菌体增殖液(效价1010pfu/mL)和宿主菌增殖液(106pfu/mL)各2ml加入至100mlLB肉汤中，37℃、180rmp下培养，每隔1h取3ml测定其OD值。另设阴性对照组为100mlLB肉汤；阳性对照组加入2ml宿主菌增殖液(106pfu/mL)；共测定12h。结果如图4所示。
由图4可知，沙门氏菌噬菌体RDP-SA-19009在体外抑制其宿主菌BS-19009可长达12h。
实施例8噬菌体裂解谱测定
采用点样法进行宿主谱的测定：取0.1ml培养至对数期的菌株加入到温热的半固体培养基中，混匀后倒入预先制备好的LB琼脂平板中，待凝固后，取0.01ml效价1010pfu/mL的噬菌体滴加在上层平板表面，干燥后置于37℃培养箱培养4～6h，观察裂解情况。宿主范围如表2所示。
表2噬菌体裂解谱试验
Figure BDA0003071235400000062
Figure BDA0003071235400000071
注：-噬菌体不裂解；+噬菌体裂解微弱；++噬菌体裂解优异
由表2数据可知，本发明沙门氏菌噬菌体RDP-SA-19009对鼠伤寒沙门氏菌、斯坦利沙门氏菌、肠炎沙门氏菌、肯塔基沙门氏菌、甲型副伤寒沙门氏菌、乙型副伤寒沙门氏菌、伤寒沙门氏菌、山夫登堡沙门氏菌在内的致病菌均具有较好的裂解性能，表明了本发明沙门氏菌噬菌体具有裂解谱广的特点。
实施例9噬菌体80℃热空气处理实验
取噬菌体增殖液(效价1010pfu/mL)1ml，分别在80℃热空气中处理10min、20min、30min，测定其效价；结果如表3所示。
表3
Figure BDA0003071235400000072
由表3可知，本发明沙门氏菌噬菌体RDP-SA-19009在80℃条件下存活30min，效价依然保持在1.0x106 pfu/mL，表现出了良好的温度耐受性。
实施例9噬菌体的裂菌效果
在LB肉汤培养基中分别制备低浓度(1.2×102CFU/mL)、中浓度(2.2×104CFU/mL)和高浓度(1.1×106CFU/mL)沙门氏菌菌悬液，按照10％的体积比加入效价为1010的噬菌体增殖液，在4℃条件下放置8、24、48、72h,37℃条件下放置2、4、6、8、10、12h,分别取样涂布LB琼脂固体平板测定宿主菌数量的变化，以未添加噬菌体的各浓度宿主菌为对照。结果如图5和6所示
由图5和图6可知，噬菌体RDP-SA-19009对中低浓度宿主菌有良好的裂解作用，在4℃下对中低浓度的宿主菌可以完全裂解。在一定时间内对宿主菌有抑制作用。在37℃下对低浓度的噬菌体可以完全裂解，同时能较长降低宿主菌的浓度。说明该噬菌体在中低温度下中低浓度的宿主菌有良好的裂解作用。
应当指出的是，具体实施方式只是本发明比较由代表性的例子，显然本发明的技术方案不限于上述实施例，还可以有很多变形。本领域的普通技术人员，以本发明所明确公开的或根据文件的书面描述毫无异议的得到的，均应认为是本专利所要保护的范围。
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<110> 瑞科盟（青岛）生物工程有限公司
<120> 一株高裂解性沙门氏菌噬菌体RDP-SA-19009及其应用
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 87591
<212> DNA
<213> 2 Ambystoma laterale x Ambystoma jeffersonianum
<400> 1
ttaatcgtaa attgttgtac cttcaggaac acacttcaca tcaaagccta agaacttact 60
cacttcgatg ggtgacactt tattccaatc taaatgagaa agcaagaagt tttcttgttt 120
tctttttgaa aggtagtttg tcaccttaat gaccatacgg tcaccagctt tcttaatttt 180
cttgtacagc tttgtatcat tgtcaagaca ccttttcaaa tcttgcaaca cgctaatagt 240
gtaagtgtat gcaaacggtg cagcaacgta ttggatttta ccaaacattg cctcaacagc 300
atcttcatta ccttctaaga agatagtttt gtttgtgtct cgtgaatagc aatagccacg 360
agaaatcttc ctgttattga acgtaaagtt tctggcaata atccaactgc ttgtaaggtc 420
taggacacca ttcatataga tacgtgtcat atacctgtta tgattaatcc agtgaacatc 480
agttaagctg tcgttcaaga gcttctcatc tacttcaatc cagtcttctg gtatcttttt 540
ccaatttgct tttcggaaaa catagactgt ctttccaatg acattggcaa ctgacttagc 600
tacatcatct ggagatgagc aaaaacattc accatcaaca gtatcaccta ctgccttgat 660
ataaagttga ggctcttcaa ttgtgtctaa gtcttctgac acctctgtgt atgaagctac 720
accttctgct ggaacagctt tccagagttt tacaacacct cttacagctt ctttacgttg 780
ataatggtgt tccttatcag acatcttcac aatctttaac aagcttttat caagtttgtg 840
tagattgatt aggtcgtcta actctttttc agtcgagaat acaaagacaa taccattgta 900
tctgtgaaac atactcgact cactagcata gtctcgacat gcaccacgca gaatctggtt 960
gcgtcctaca gtcttctcag taccgttttt attgcgacgg tcattaatca cgaacaaaaa 1020
ctgttcaatt tggcttttac gcattgcacc aaagatattg aacatacttg cctcttgagt 1080
gtaggaaagt gctgttgcac gaatcttact ctctaaagag ttgaacttga cataagcaac 1140
tgggttataa agataatcta ctttagggat gttgtttccg ttcctatcaa ccttaatatt 1200
acctttaccg tcacgttcat aaatgactga gccgtcttct gcgtagataa tcccacgacg 1260
aatgtttagc aattcttctt ccagagtatc aagtttaaca ccaccccact ctagctttgg 1320
acacacagca ttaaacattt ctcgtgagtt caaacgcaac tccgcataag cctgtgcagc 1380
atccataagt gtaggttggc tattaacttt cttgataaca tcctttgtaa ttgcttcagt 1440
tatcttttta gtagcctcaa tgataacatt ttttgtcgta tcattcatct gcaatgcttc 1500
acgagaagct gcaatagcaa ctgaaccaat aggcatgtaa atgtttacaa ggtctacgct 1560
cctacggaaa aattctggca agactttgaa gaaatcatca ccaagtaatg cttccatatt 1620
aactggatag gcaatattac ccattaccac attaaaatct gtcctgttgc cactagaacg 1680
ccagctttgc ttatgaatca tagcatcata aacacctcct tcacgggcaa tgacattcat 1740
atctgctaat acatcatcat atacaatatt actttctggt tttacagcaa agtatgagta 1800
tacgttacca gcctcttcaa agaactttga aatacgatgg tcagcaacag ccacacgtac 1860
agctaagcca ttaggttcat ttgttgggtt agtagtaagc ttagtcactt gagggatacc 1920
attctcaagg taaacagagt atttattaac aacaccgtca acatagctag acactgtgaa 1980
tgactgagca attgcaaacg gtgactttga gccgataccc attgcaccaa tgtagtcatt 2040
agagtcgttc ttcgtagaag ccccgtagtt caggtacaaa ctcattactt tgtcatgggt 2100
taaaccagtt ccaaagtcac ggacttcaaa gtaaggctca aatcgagtag gtaaatgcac 2160
atggaatggg atgttctctt ttccagcttc tttctgagca tctactgcat tgcatgacag 2220
ttcacgaatc actgcccttt ctttgaatgt gtatacacca gaactcagaa ggctgaacat 2280
ttcaggtgtc attgtaatct gtgcttgaga tgtctccaaa gaagttgaac tcttaatcac 2340
ttctgcgtgg tcgtttacca tgcgcataat actttcctct tagtttgctg ttaaattact 2400
tattaaacat tttaccagaa ccaccacaaa tagggcaaca acctgctctt gtgtaccctt 2460
ctccgttaca gtagtcacac ttcttgtgct ctgcatagta cttgcaaaga aggtagacaa 2520
acactgctac actaccaagt gacagtaaaa tttctatcca atatgcttgc atgactcacc 2580
tctcatgttt ttgtaaagga taaagcttct gtcatggacg atacttgtag acactgcaaa 2640
gttactttca agtatctgtt tgtttggatt gtagaagctt tctttaaact cgtcaagtgc 2700
aaattggtgg ttgtctggaa gaccttttac acagactttt attgtgattg ctgctgcaac 2760
cttacccgtc agttcttctt tagctgctac taaaattttg ttaaggaatt tatgaccatt 2820
ttcaggtttc ttaatgtttt cccaacgttc ttccaagaca acgttaatat tcatctcttt 2880
gattttcatg tctttctcca gaaattaaaa aggctcccgt aggagcctct tatcatatac 2940
ttttaaagct gcttgtcaat attgtttcga atatcaccaa gagttgtata accgaactgc 3000
tcagagtcgc taaagactaa acgtaaagca caagctgggt ggtcaagtac atctgcaaaa 3060
ctccggaatc catagccatc tacagctttc agcttatcac catcccacat gggtgctaca 3120
ccaccaaagg cagacttctt aacaccacta tctgttttag ggtcttttgc aagcataatc 3180
tctttaccac caatgcttgc aagagttgct ttgacagcaa atgcaaaggt gtcacgagtc 3240
atgtactggt aagtgtaaga gcctacacca aacactacgt tagagcttgc aaaacccatt 3300
tcatacagac gcttcaggat ttcgtttgca cgttccagcg tgatagagtc accatagata 3360
agaccaatat gctcatccag taccttaaac cctttagagt tgatagttcc cccaaagatg 3420
ttgtacagtg tcttaatagc tccatcaatc tcagctacag gacgtgttac aacgtttgca 3480
gaaccaacca tataagcatt tttcaacatc accgtatcag caacttcaaa gtcttctctg 3540
ccaacaacca tttcgtagcc ttctgacagt agccatccag caataccgta actaatgttt 3600
tcaagcttca tgttcaggac agagttcagc attgtatcac ttgcctctag cttgcttagg 3660
tgctcgtagt aagccttttt agcacactct aagtggattg ctttataacc tgtgacaata 3720
tgtacagggt ctccagagtc agggcgaatt accagcttgc catcacgttc cataatttct 3780
ttacgaagtt ctggtaagat ttctgacaca gttctccaga agttataagt atctgaaaca 3840
atacttgcaa taccagttgg gtaagtttct gttaagaaac gacggaaggt ttgcaactca 3900
cctttaaatc gtctttcttc ttcaaccaac tcgttaccac cttcccaagc aatgtttgca 3960
cacatcacag agtgttcggt ggctggtaca gaactaccaa tatcagagat tgggtaagac 4020
tgtccataga tgcgtttagc tgtgtatata gcagggaagc tatcagtccc tttaaagctg 4080
gttaagtgac ctacagcatt aaatgcgtca tcagtaaagc cggacatacc acgcatagca 4140
aagtcatggc actgataagg taaatgtaag tcgttgtcgc aagtaaggtc agcccacttc 4200
ttacagatac gtttgtagtg caatgcgata gtcgcaatgg tgcaagcctt ccaaatctca 4260
gcagagaaag catcttccag ataacctgct acccaatgga aacctgaaac agtgttctgg 4320
aagacaatca ttggaacacg catagggaca actgtgcctt cttctacagc gtatacttca 4380
actggtagat aacctaagtc gtgaagtgct tcccaatgct ctcgaccgat agcatcttta 4440
cccagaacac cattcatgac ttctaagatt tcatcaattg cttctttttt atcacgttca 4500
aagaaagtgg cgttccagtg gtctatcaag taatctttaa caaaacgttg aataccaaaa 4560
gccactacac cgtcaattgc taaaggactg ttaaaccatt tatcactacg tggtgtcagg 4620
ttcaacataa gatattgcgt agcatctgga tactgatata tatgtgatgc cttgtaagca 4680
tctgcattaa gacctgctgg tactgcgtaa agtgacttac tcatatttta tctctctctt 4740
agttatgtct tgcaaactca ccatgaagtt ctattcgctt tttaattaca gcttcttttg 4800
cttcttctac cgttttaaat cttccacaaa agtattcttt atggtaaagc atgactcttg 4860
ctttgtaaag acctgtttgt ttacaaaaac ttacaccctt cacaccagtt ttattattct 4920
tgtatatacc cctattcctc tgattttgtg actttgtgct aagtcttaaa ttctccaact 4980
tattattaga tttatcaagg tctatgtggt ctatctcata tccacttggt atctcacctc 5040
cagacataat ccatgcaata ttatgtgcat agtacctctt cttatttatc tggacttgga 5100
tataaccctt cttagacctt gtgccagcca cattaccagc aatagcggta ctgcaaaggg 5160
ttactctcca aattaataaa cctgtatctg gttcatatat taggtatctt tcaaaataac 5220
ccttattcat actgttacct aatcaaggtt tgctacagta acttgactgt aatgtgttaa 5280
gccacggtct ttagcttcgc ctaaagagtt tgtagtgtaa atatggtcga taccattgtc 5340
aagaaggttt tcaacatctt tagagaagat accatgtgtt acataaagtt ccacacgttt 5400
tgcacctgct tcacgaagat gtttcgctgc ttctacgaag gttcgaccac catcacaaat 5460
atcatcaaga atcatgacag ttttatctgt caaatcaaca tcatcaagga ttcgcatacc 5520
agttatttca ccagtcttaa ggttgcgtac tttagacatt gtgatatatg gtttatccac 5580
ctctttagca gtctctgcaa tcttcttaga agcacctgca tctggggcta ctaagtaatc 5640
aatcagaggg tcatttgcat agtggactgc aatctccttt tgaggaatac tctggaagca 5700
gttgaaaagg ttatctggaa cgtaactatg agggtcaact gcacagactg catcaaaacc 5760
cattgcatta acctgttgtg caaacacttt caaagctgct gcatcgcctt taaacatgtg 5820
acggtcgtaa cgtgcatttg gcaaatagta aaagatagca gtcttcatag caaacttacg 5880
aggtactaaa tcatcaattg cctctttagc aagtgccaca gcaaataatg tgtctttgtc 5940
ataacccttt acaatcataa tcacgttgtt gatggatgat gcagcataag cagtaaaatc 6000
aacaaactct ggtgaaaaat gtccaccaat ctcaccagaa ggaaattgga cgatgttaaa 6060
ctcttcttca tgagtccctt tagttggtga atgaactgta acacggatag atgttttcat 6120
agttttctct ctcaatcaaa agttacgttt gttactgcca ttgcagattt gcattttgca 6180
gggtctacaa cgtgataaag tacactacag ttacctgtgc tgtctggtac aggctttacc 6240
cattgctgct ttatagttgg ctcccctaag tctttgtaag agccatctgt aaagttgttt 6300
accatctcaa gtgcaattgg ttctggaaca tgaccacgta tcatgtacat ttcacaacca 6360
ttaccacctt cactgactaa tggtaaaatc ttccacttct ctttagccat tataagttgc 6420
cctataaact gtctcgaact tcaacaggag agatattgcc aaatctgtat agttgttgtc 6480
aagcacatct ggcatattat ttttgatata tttctcttgc tcttcatagg tcatcttagg 6540
tgtgcttaga aggttatctc cttgcttctg gttatcgagc ttagtctgga taaaccagtt 6600
tgcacagtga tgctcaccac cctttgtaac aggtttgtgg tggcctaatg taaatgtttg 6660
cttacctgta tacctgcaaa gattgttcag tgttgcaata gtgttcttca caacacacca 6720
ttcatacagg ttatctattg tgtagtgtgg gtaataacgg acaagtgctc tttcacggaa 6780
gttatcacga ttccaaagaa ccatgtgatt agagttagaa gggtcgtatt ggtgggagtc 6840
aatgaactct tgacgttcct caaagctgag tcttagaact gacattgcag ccgaacgttt 6900
caggtttgac agatacattt tatctccaaa ataaaaaggg aacctttaca gttcccttat 6960
agtatctgtt tactgagcta ctttcaagat gcttgtgaat gaaatctcac tctcatccat 7020
aaactctttt gcttctccag taacttttac aaagttatct aggcagataa tcttcttatc 7080
tttcttgctg taggttagtt taaccttttc aatatctttc ttagagcttt tctcttcatt 7140
aaagagtttt acagcctctt tgaatgggag gtatgagcca tcatcatcac taataaaacc 7200
tgtaccacaa tagtagaagt tatattcttt agccataaac tggcacagta cgaaaatctg 7260
taatttgcga gtgttataaa ctttgctcat gattatttag ccttcttatt acgtttacgg 7320
ttacgagctt tcttagctgc tcgtttaata gctgctgcac cagacggacg gtgggcttgt 7380
ttcttaccac ctttgccacg gcctacatag atactttcca taatctcatt agccagcctc 7440
tcatccatct taaagttctg cataagatga gtcacatcaa caccagtgag catacctaat 7500
gaagctagtg ctgcttttac ttttgattta aaactattcc agagtcccat ttcatatctc 7560
tctcaatctt ttcacaaaat gttttaagct cttttcagtg acacaaacgg ttaagtttgt 7620
tctgtagaac attgtaaagt gtcctttacc gataaactac tttacgacga gacttactcg 7680
gctctccact gacaatcacc ttgccaccca tactcttttc tccagaggtt ttggttatca 7740
gaaccacgat acggtttaac tgttggttta gatgaatcat acttaccatc aatcaccacg 7800
tcaacatatt tcattacatc taggtgaatt ttttcgtgta actgaaaacc tgtccagagc 7860
cagatagact tctctgggta aacagtttta atacgtttac atatattggt aacctcttga 7920
atatttctat catccagagg ttctccacca agtattgaca aaccactgac agcattatca 7980
cccattagct ggatgattcc gtagaggttt gcataagtaa acactttacc agcattaaac 8040
ttccaagact ccctgttaaa gcagccttca cagtgatgct tacaaccagc tacgaagagg 8100
cttacacgaa ccccttcacc attagctgtg tcaaatggtc taatctccat gtaattcatc 8160
tagttacctc accaataaaa aggtgatgta agcctatcaa gacctacacc actttgtcaa 8220
gttttacata aggttatttt ttcttacaat tgtcaaaatg atatcgagtc atgttgccag 8280
cgccaccagt catgccacaa tgaggacagg ttttcttttc ctttggcttt tttccggctt 8340
ctgacatctt cttccttgtt tctatagacc ttttacaacc ttttgatttt gtatttccta 8400
tttgggctgc ccctaagcgt cttcgatgtt cttcagtgaa aaccctacct ttattcacaa 8460
tggatgaagc tttcgagttc aaagccctta ccttttcata gtctctggat gatattttcc 8520
tatctaggcc atcatgagct aacctgttcc atgcaaacca taacttatga ttgtttggat 8580
acatcttaac cagtaattta tgagctacat aatgctctct aggtgtaagt aaagcaatat 8640
tatctccact aattctatcg ccacctagac atactggtat gatatggtgt ctttcataac 8700
ccttaccttg acatttgtag tatgtaagag gcacacccct ttcagaatgt gccttcttaa 8760
taattgcatt atatattagt tcgtaattca tttatatgct acatagaaat cctatcctta 8820
atttctgcca tcttagcgtc attcattcga gactgacctt taatttttgt gaatgacaag 8880
tacccgcaca ccctgttaat cacagagata tcgtgtgaat gacaaacctc acattcttca 8940
acatcagcct ttggtctgtt gccacaatgc tcacagattg ctaagtcaaa gttaagtccc 9000
tgatagaaac ctttcaacat ccctcttgta atacaactct taagtgctgg taagttttct 9060
ggattagcca ctcttacata ctggattctg ccgcctctac agatatggaa gaagggttct 9120
tctaagtcct gcttctcaaa tggtgtaatg tcggctgcaa cattcatatg gaagctgttt 9180
gtaaagtatt ccttgtcaga aacgcctttg ataacgccaa acatatctct gaactgtttt 9240
agctgagttc cacaaagtga ttctgctgga gtgccataga ctgcatataa gaagccatct 9300
tcatttttaa actcttcagt cttcatgttg atatattcca gaacatcata tgcaaaacta 9360
cagcttccaa cttcatgaag tcgtttacct tcagcaagaa catctagctc atcaagcgca 9420
gtaaccccaa aagaagctgt gaaggacttc acgatatccc agccaacctt gtcagtaggt 9480
ttcttattcc ctttgtacag accaccttgt gtgaatgcaa gagggttaga acttgctggc 9540
atattagcaa tcatttcgta acgtttcttg tggaagctgc gaatcatttc taggtactta 9600
tcaagctctt tccagaaatc taagccattc tctttagaat actggtaaat cattggtaag 9660
ttcaaagata cagcaccaat gttagcacga cctacataaa actcttcacc atattcgtta 9720
tggtatggtg ataaaaatgc tctacacttg ttgttcaggt aagttcgcta agcttacccc 9780
gctttattca agctgcttac tgtcgccagt aagtccagac tatatcttct acttatttct 9840
aagtagcgta ccatttcgag tcgcttgacc ctacaccgct acattcatca cggttagtcg 9900
ttcggcattt agatgtattc aaactcatat tcgaagttat tatgctcttt tacacccttc 9960
aatattgaag agatggtctt gcgattgtgt ccaagtatat tggataactc tcgtattgac 10020
ttagcaacaa taacttcctt tgtcactttg tgtgtggctt tcacggcata tgaacgcttg 10080
tggaacttgt agcatccgtt ctcataacct tctttagcat tctcagaatg agtaccccat 10140
tctaagttag atgcgtggtt atttagtttg ttatcgtcct tatgttttac aattggaaga 10200
ttatttgggt taggtacata tttctcagca actaacctgt ggacatacat gtcacgattc 10260
ttcttaccgt aaaacctcac tttcatatac ccagtgttgg atttccactg cgtaagctct 10320
acaccatttt ccctaaagac ttttccatct tcagtgacgt gatagtttgg catgtaagac 10380
tcctatgttg tatagtttct tacatcgtac atcatttagc acaggattgt ctacatctgt 10440
agagtttccc tgtttaggca cgttttagat gagctataga gcctgttaac ccatcgggga 10500
aattaccttg ccagaacgct caaaagcctc tgctacagca ccatgtccag atacacttaa 10560
gaaatctgga tacatagctt tagagcaaca ctcaatagct ttactgtaca ggtggccttg 10620
acagatattc tcattgtgtc tcttctggtc ataaatataa accaatttag ggaatacaac 10680
aggcttcttg ctcttacctt gtccattcat acgaacatct aggagagtgc tcgcaatcat 10740
gtattgcagt cggttatctt cattagacat atctgagtca agcaacccga atgtcaaagt 10800
tgtgaatgca aaatcaccac ggctacaagg tacagtattt agtttcattt caagagactg 10860
aaaaccttga gttaattcaa tctgtagctg ctccatgaca tagttgtggt aatgttcttt 10920
aggaatacca taagctgttg ctttctcagc atgatagcgt agagacttct tagcatacgg 10980
cacaagtacc ttatcaatct ctgctaaagt aaagccacca aattgctgtg cagttgctga 11040
aagaactacg tcaccaataa cctgtaaggc tgacagtaca gacttcggtt cacaatattc 11100
aatgccagac atttcaaagc cacctttcag gactttacca atgtcaaaca ggcaacagtt 11160
gataccacca aaaattaggt ctcttaaatc atggatatag ataaaccctt tttcaatggc 11220
ttcaagttcc tctggtgtta agtggtactg tttaaagatt tctttagtca gataaccacg 11280
aataattgaa ccttttgtag aaattaaact gctgtcaaag ttagcattct ctctgtcacc 11340
taagaagagt gtatctttgg tcttctggta gagttcatcc caattttgag caacttcttt 11400
acggtaattt ctatatgttg agtaagactc ataaatctcg tgattgactt ctgccaaagc 11460
accctcaaca atactgtgaa tatcatttac agaaattagc aaattattct gcttagtgga 11520
ttttaccaga agcctcataa atgctgactc aagagcttgg gtaacatctg gtgggagttc 11580
tttataccca accctactag ctgactttgt gactgctgct aaaacttttt tgatatcagg 11640
ttcttcgagt gagccattct ttttaataat ctgtactttg ttcattattg cccctttaca 11700
tgctaaaaag gtctccgaag agaccctttc attttaaatc tttttagaaa acttcgcaaa 11760
gagttctctt agtttttcag tgatacctaa ctgtatctct aatatttcga agataaccca 11820
tagtaacatg gctccaaaca atgaaccttg ttcaaagtca gtaaataggt taacaataag 11880
cataccaatc acaatagctg gtgcatcaac aaccaaacct tcccaaatgc gtttaagcat 11940
tattcacctc tgccataaaa ttctgtagag tcccaacagg ttttaggttt tgtccatcag 12000
ttttcatgat gaatggcatc tgacgaactg gcatctgtgc aatatccatc aggtctgata 12060
gctcatagtc ctgccctaac attctcacaa catggtcaat accacgagct tttgcaaaat 12120
tctttgcagt ctcacactga gggcatccag ttttggagta aattacataa gtcattaagg 12180
aacctctaaa aatccactat tcaaatcatc tacaacagta ttcaataggt acgcaccgtt 12240
ctgttgctcc tgattagcat tctgctcttt atcaatttcc atcttcttaa tcatatactt 12300
taaaggtggt tctttaggag ctacaaaatc tctgggaatg ccaaacatat catacagtgg 12360
ggcagcatta tagtaaaccc actcatgaag aagctttgta tttaagccaa ctacagcacg 12420
cccttcggag aagatataat acgaccattt ctcttcactt tcaactactt catctaagat 12480
tactttaatc tctggaagaa tttgttgaaa agctttctgc cactcatcat ctcttaaagt 12540
ttcttttaaa acttcaatat caattttagt gtgaaggatt tcgtcaagca taatcttctg 12600
gacagcttga gcaataccct gaaatttatc ttgagcatca agtgcaaagg tacatgcaaa 12660
ggatgccata aaagatatac cttcaagtgc agtcactgca aacagacctt ttagaatcac 12720
tttatggaag tgtaaagggt ctttgtccag aagtgaatca cggacataac tcagtcggta 12780
gtttatccct tcatccagta attcttcaag aacacgattc acagttttta atcggtcttg 12840
cacagcaaca ttctggttaa tctcatctaa gattgtttca gggtttttaa tacattgtct 12900
tacaatctct gagtaagtaa gagcatgtag gttttcaatc tcagactgct tcataattgc 12960
agttgcataa atgtcatcag agataaaggg tgcaaaggca aatgccaagc tcttagcaac 13020
ttgagtatct gcttcccatt gccacttaag aatctcaagc attacacctg acattgatgc 13080
tgggacactc tcaaaatcaa gacgtgattg ttcaaaaggg aactcatctt ctgaccaatc 13140
ttgtgctttt tgttgtttat aaagctcaaa gatttttggg tagtgtttat taagtgagtc 13200
aaaagttttt ctctcaccac ctaaaaagat tgggtgctgg ttaatcataa cgtgatttct 13260
cctttcttta tcatttctaa ggcttgtttt ctgtctaaaa cttcccaatt ttctttgtta 13320
aacatctcat aaggtcttgc atagatttta ccatctgctg tagagatgta agagatacca 13380
gcaacccatg agtcatcatt ttgttttatc atcatgtctg tgctactcac atagtacatc 13440
gttttccgag gcttgtggag caagtatata ggtcgctcat gtttttctat taaatctttc 13500
atggtctctc caaagttatt tttagagggt cttttacgac cctcatagtt gttaaaacta 13560
aacaccacaa ccctcgcaat aagcatcttg cagtgcagat ttacctacac caatgcgact 13620
gttaaggtag tacatggttt tcatacctac cgagttggca taaatcatgt acttcagagc 13680
ttgagccaat gataccttct tagactttgc atagtcaaca tagaagtctg aagagatagc 13740
ttgaccagtg aacttttgaa caattgcata acaatcaatc atatcaaagg tgtcaatatc 13800
ccaagcaatt tcatagacat acttcaactc ttcataatct ggaacaataa acagtacgtt 13860
accagttgca gactttttag ttaaaataaa gtcacgaatt gggtacaagc catttgtcgt 13920
attagttgct agtgaagaac tctcattagg catgtaagct tctaatacag agtttcggat 13980
tccaccattt tctttgatac gttgtgctaa gtcatcccaa tcatatctta gttttgcatc 14040
atgcttttca tcaatcttct tgttagctgt ctttggagga acccagcctt caggatactt 14100
agtgaacttc atgtactctg gcacgccacg ttctttagca agtctcagag aggcttcatg 14160
cagatagtaa gagtgcatct ctgccagttc atgaagtttt gttttacctg ctcgtgaaga 14220
gtagttcacg tagttcttcg caaggtaatg agccacattt gtaaggccaa tcccaacaga 14280
acgacgcttc tgaacatggt tacgcatcga cggatacgga taatccataa ggtcaataac 14340
ggagtcaacc attgcaagag cataataagc aacgtcagcg tattcatctt ctgaaattct 14400
ccctgcaacc aaactagcta ggaagcaaag agccacctca ccatcctctt tcacagcatc 14460
gtctctgtaa aggtctgtct ctttctcaaa accatacact ggcaacacaa tttccataca 14520
aagatttgac atcttcaatg gttctttaaa tggtgtatgt gtgttcgcat tatttgtgaa 14580
gaatggatac acacgacctg tagcataacg ctgttgaata aacagcttag caatttcacg 14640
agcctttact cgtctgtgct taactcctga atgtactgcg tgaccaactg ccatagcaaa 14700
ctcatcagca gatgctgtgt agaacatgtc atagagtttt ggtgcatctt tgtaagagaa 14760
tagcaaccaa tctgtatcat actgaacaca ctgccagaaa taatcgtttg tgccgaatga 14820
gtagtccatc tcattaatac gtttagaagg aaccgttgta gggtgcttca aacgcagtaa 14880
atcttcaatc tgcgggtcta gagcagtgta gaagttatta gctgaaccac cacgactctt 14940
ctgtttgttt gcctctacag atgaacgtac aagcttgtaa taaggtagct tacccatgtg 15000
ttcaatcgta ttttgacgga taccatcacc aatagtgcgt gtctccatca gcataccaat 15060
accagcttgc tttgtggtca tatcataagc aacctttgca gcaataccga gagactcagc 15120
agtatcattt gccttaatca aacagcatga cgcataacct gatttagttg ctcttaaacc 15180
atttaaataa ggcgtaggag cgttaatctt caggtcagat aggtaagtgt acagcttgat 15240
aacatcttgc agtctacggt gctttggttg cttctcaaag gctttcatag ccatacccat 15300
aaacataaat tgtggtgact caaaaagtct tcccgtttta atatcacgga taccatactt 15360
gtctctgaac tgtttcaaga ctgcataacc gtaagagata tcttttgagt gtacgatgta 15420
accttgcagg tattcaagct cttcctgtga ataatccatc ttctcccaaa gtcctgctct 15480
ctccatattt ttaacgaagg taaccagcgt aggaacctta gtaaagcctc caaaggcttc 15540
tttgtagata atacccaaaa gcagccgtcc agccatatct gagtactctt gagtttgttt 15600
atcaacacaa acatcaatca tggcttggtg catctctttt gtagtgcaac cctcatagac 15660
acgtttcatg gcttccatag tgacttctga ccagataatt ccacgcttat ctgcccatga 15720
tgcccactta ttcagtcttt ctgggtcaaa gcttactact gtaccgtttg attttttaat 15780
tgtcttaatc attttcgaat cctacagatg aaaagagcct ccgaagaggc tccctagttt 15840
aaatcttgaa aatttgctca ttctgccaca tatcgtaaaa cttatcactt actcttgtga 15900
ctgaagaagt gtgcatacaa ttcagggaga accatgatgg tgtattatca tccataagtg 15960
tttcaagttt ttgtgtaacg ttcacatcca tgtctgcaac aacgtaatta cctcgcatct 16020
tgcaaacaat tcttccaaac aagattgcac ttctgcctct tcggtcatca cagtacataa 16080
ctgtgtcccc gtgcttaaca tcttgtccga tactatcaac acctaattta gcacctgaga 16140
taatgtcatc ataagatagc ttttttgctt tagacacggt atttctccaa gtttcttttt 16200
agatacaacc aaccatagtc agtcctttct acacccataa agagtggagc catcacaact 16260
ttaccgaaag tattcctgtg ttggcagatt tcaaaggaat actctttctc aatatcaatc 16320
ccgtatttgt acaggactgc ttttaaaact tgttcattgt ccagaagtgt atctggagtc 16380
tcaccatatt tttctagtaa cgggtcattc ataaacaccg tcatagaaat attgtagttt 16440
gagaagtggc tcttctgagc cacaccctct ttattaatgt tctgtgaatc cattataacc 16500
aacccctttt aaccagcact tcaggttatt agcatcataa ttcagtttcg ggtatgaggt 16560
tacatggaac tgctcaccat catagtagac aatatctgca atccatagtc cgttctcttt 16620
acagaaatct ttatctgctt tagaggtaat tggattgact tctacaaaac catctacacc 16680
caaatccttt aagctaccta caataccttt gcagataacg catgtctcag atactaccac 16740
aaaaagttct tggctttcaa gctctacaat gtattcacca agctgtactt tagggagatt 16800
tgattgcttc ataatcttcc catcggctgc acgaacaatc gcataccact ctttaccatc 16860
aactactgac attctgataa tacactcttg accagtacgc ttttcaatgt ctgcaagacg 16920
ttttaaagct tcttcataat catctgtgta cttcaggtta ttattatgac aaacagcttt 16980
catagcaccg ttatggtcat gttgtgacag gtaaatcaag ccgtcaagaa catattgtaa 17040
atctgcctga gcatccagtg cctcaatggg gtctttctct tcaattgctt ttacaagctc 17100
ttttgcttct tcaagcatac acagagattg agattttaaa gattcccaat actcatcact 17160
gtaaggttgc ttttgagtgt ttccacaacg aaggttccag ttttttactg attctcttga 17220
gttaaacatt cggactcctt aaaatatttg ttaaccttta gctctcgtgt ttcaatatat 17280
tctagatgct cacgtagctc ttgacgttta agcattaggt gctgcatttg agattcaaca 17340
gcttcaactt ctttaattat tgaccctcta gcatttgcaa ggatgcgctc aagttcttca 17400
atcttgctgt caacttttgc tacatgactt acagctttac ttttgttaaa cattttattc 17460
tctctcaata gttatcaagc acaatttgct ttcattacct tatggattct tttttcagca 17520
tcttcataag tttctttggt aataaactta attgctgcaa tattggcatt gtagaagagt 17580
ctgagcttcg agtcaatcct ttttgtcatt acatcgaact tatgttgaag gtttgcttca 17640
ccataaacta gaccaccttt tgtgtagtag gtttggataa tgtaaaagtc aaaaaattct 17700
tttccaaagc tttcaatatc ttttttgata tactcagaag aagtctcata agtcatccaa 17760
tcactttcct tagtgactat cttcttccga gtcttaccag caacttttct tttggtcaca 17820
ctgttaagct gttttttacc caaatagtac tgtccagtct ttttgcagta cactaggtag 17880
acaaaaccaa agtgctttgc tgggtcaact tcaccacata gtgaaatcca atgtccataa 17940
gttggacaac tcccaaaacc tttaacttta atcattcatg agcttctccg tgatttgtaa 18000
actctctatg aagttcttct cttttctttt tgacaacttc ttcagcctca gcaacagagt 18060
caaatagacc tagatggtag caaactttat tatgcttaac ttgtgcagcc catctacctc 18120
tttgcttgtg gtaatataca cccttcacac cagacttatt atctcttctt ttgactgcat 18180
tacagttgtt gtctgacctg ctacactctc tcaaattgtc gaaccagttg tcgtatctga 18240
tacaattctt gtggtcaaca aattttggga ggtatccttt catatacaag aatgccagcc 18300
tgtgtgctgc atatcttttt ccgaagagtt ttatatagat ataaccgcta tggtgtttaa 18360
aacctgctac tgagccagct ttggccttac cttttctatc aactaaccaa gtaaagaccc 18420
ctgtttcggg gtcatagtgc aggtgctctt taagagtttg ttgagttaac atttggtgtc 18480
cacccatact tatcaaaagt gaaatcgtct ttcttatttc tttcctgata agctaagaaa 18540
aagtgttgct gcattaactc taagggtgtt ctggtaattg tttgaccatc ccacgagaca 18600
taagtgtacg actctttctt agcataaagc tcataaattg catcaagaca ctctttgtaa 18660
gtggttttac cctcaagggc tttcattata gtaaccttgc caacaccctt taaaccaaaa 18720
taattatccg cattatcacc agccacggct tgatagcaaa gaaatttaaa acctacccca 18780
actgtcttct tagcttttgg tgttgatttt attgggcaat cccatatttc accaacattg 18840
ttatcagcaa taaaaattaa aggtgatttt tcataggtca tatcaatgca gtaagtgcct 18900
tcagcttggc gaaggtcttt atcaatactc attaaggcag cctttttacc catcttttca 18960
gctttagcaa ttacgataga gtcagcttca aacccatcaa gaatcaattt gaactctggt 19020
cttgacagaa gatactcacg acaagcaact aagtgtgttg gtgtgacagc atctttacgg 19080
ttcccttgat attggtgctc aagaccttta atgtctttat gtttatgcac acctttctct 19140
gttaaataac ctacccaagt tctttcttta ccaacaatct taagccattc ctgaagcacc 19200
tgttgagtag ccatgatagc ttctttttca ctcttagctt ctttccaagt ctgtctttcc 19260
cattcatctt catcaaatgt caggccaagc tcttccacaa ggattctctg gtctgctaac 19320
catcttgcag catcttttgc attatcaaac gaatcagatt cttctgctgt gagtttattg 19380
acatatttat attttgcttt ctcaactaca caagcacctt tataggcaat actatcagag 19440
tcaataaaaa catgtgtaac tgaatcggga agttttgtta atgtgtactt ctccattatg 19500
gctctccata tgaaaaagcc ccatactaag tacagggctt taaagtaact tttagagatt 19560
agtcttctgt atcgaagtct tcatcttctt cgtcatctgg gtctggcaaa tcttcatcat 19620
caccatcatc agaagcgttc gaaggtttat ggtctgttgc gtcttcttca gtgatttcac 19680
cgttatcttc aacaccatca agaccaagca tagccagttc atcttcatca agttcaggtt 19740
caccattagc accgttacca ccagtgtaag gtacaagagt atcaatgata aactgttcct 19800
gaataggttt tgtcagaaca ttgttctcaa aagtgtagaa gtgagtagaa agaatcacac 19860
taccaaaaga accgttacca actgcgatat ctggatgaat tacgtcatag ttcttgtcat 19920
cttcatgttt atcagatgct tgagctttga ttttcttcat cggctgctta acagctacac 19980
gcttaccatt tacttcttca atcagcatta cagggaatga ctgtttagct gtccatacag 20040
caccatcttt ataagctgct gcacgactta ctttcaagat gtagtaagtg tctgcttcaa 20100
aaggtggctt acaaccaaac ttctcttcga agtcgtctgc atcaactgct tcagtagtaa 20160
ctttgtccca accttctgga tttttcttag acttagtaaa ctctttaaac agtttgttac 20220
cgtcttctgc cagaattgat acgctgtagt tacagtcttt gcctgggaat ttcttatcaa 20280
tagatttacc ttttcccgga cgtggtgaag tgttcaggta ataaaaccag acatctttca 20340
gcaggtaacg cagagtttga cgttcagtac cgttgtactt ctctaccgga gctttcattt 20400
taacaacttt agacattatt taaacctcta tctcaattta tgaagagtac caattctaat 20460
ttgttgatac tctattgtca aatactaatt actggaagtt gtgcttatct ttagaagctg 20520
ctttacgtgc agtttttcca gttcttacac gtttctcttc atagtatttt gctggttttc 20580
cagcagtggc ttttaaggaa tccccaaaaa ctttttcaaa tgctttagag tgtttcatga 20640
tattctccga tatacttgat attcaaacac atcttcttta attataaaca gccaactaat 20700
attatgctga ggcagcgcaa tgatgactgt aatcatgctc tggtgaaaat cttcttttat 20760
ctcaacctct ccatcgcatt tcatgaggag cattctatag aacctatcaa tctttgtcaa 20820
acactcttcc tgaaatttat ttaaactttt ttcttcaaag aagggtgtga acccctctct 20880
tttataacta tcactcagtt tcgaatgggc agtcatctgc atcctcactt aatacaggtg 20940
gaatcgttga gctttgacgc tgctcttcag tatagacttc accagtctca cggtcaaaca 21000
cttcatcctc ataatgtggc aaagagtcat cgtaatggtc ttcagcttcg ccaataccaa 21060
actgttggcg aatattctct gctgcaccat caatgtcaac tccgcaacca gatgctttga 21120
tgaggcgtcc tgtatctgga ttgtaccaag tatgaccagc aatacccgtt gacttaccgt 21180
gacgacgaca tttagttagc ttgatttttg tcaagttttt cttaacaggg tctgggtcaa 21240
ccttgttacg cattaacaga atgttgttca tagaaatctg gaaatacgca ccagagcctt 21300
taatatcctc ttcagagata tccccacctt cagagttagc cttctgacca cctgcactct 21360
tacgaacgtg acagacgttc acttgtgcat actggtagcg tttgcaacga cgcaatagct 21420
cagacaaaac ttcctcttca tccgtatctg aacgtgacag agccaacgta atagggtcaa 21480
gaataataat cttacagtct aagctattaa caagatagtc aacaaactcc agtaagttat 21540
cttggtcaat tgccccttga tggtctacga tgtggatacg acgaccttta gatagttctg 21600
cgtgcgctcc cttcaattca tcccaatcac gttcgtcata aggaatctca gaaatctgct 21660
tgctcaggtg gattgcacag agcatctcca tcaactcttc ataggtatct tcaacaggaa 21720
ttacaccaat attgtaatcg gtatccttcc aagcagtgta aatcatttca cgagtgtatg 21780
cagatttacc cactgaagat ggtgctgcca gagttgtaat ctcacctaaa ccgtaaccgc 21840
cataagtcag cctgttcaaa tctccgaaag attctgggaa aggaatcaat ggaatctgac 21900
cacggttctt cattgcctca aaaccatctg cgaagttctt gataccagca gggcagtaac 21960
ggggtgcatt gtagatacgc tgcttaaatc cttccagaac tgtatctttc tctttataga 22020
actttgtcca ccattcgtta aggtctttta caccctctgg atactggaat aaacgaacct 22080
tctcaatagg gagaatgcca gcagcctctt tagtggcttt agcacctgct tcatcattat 22140
caaagcacaa gtaaatttca tcaaatgatg taatgtactg atagttgtct ttgatagact 22200
taatgtttgc acctgatgga acagatacgt gacagtaatt cttacgacga gacttgtcct 22260
taatcgcaag agaggtcata tagattgctg tcgcacattc catctcacct tcccagatga 22320
atagacggtt accaccttct ggagcaatcc atgaaccgaa cattgccagt tcacctttaa 22380
tgtctccaac accacctgag aagtctttta acttacctcg taggtgttct tttggatggt 22440
cttctgggta acggtgacga acacggtagc caacatgttc tagcttacca tcttcattac 22500
gtttgtaagt tggatagaag tgcgcatcaa tttcaccatc actgtcaatg tcaaccttga 22560
tacctaaacg ctcaagaact tttgcaggaa tcttcctgtc tttcaagtcc attgcttcta 22620
ggttttcttt tacatcgtct aaatccattc cacggaaagt acggttttta ttgtctgaac 22680
cagtagaata agtgctcacg atttgtcctt tatcaaaatc ccactctggg aaacctttgt 22740
tacaactaaa gcaagtcatc gaataagaat catcgtcatg atgatagatt gaaccagcat 22800
ctgatgaacc acaacgtgga catgcacaat gaccaataaa ctgaccagcc tctttcaatt 22860
tacgaccttt agacattagc acctcttcgt tgtagttctg ccttcaatcc attttcaatc 22920
ttgtccagtt catgaatttc atctgcaatc tctttccttc gtgattcaac tctcttaaga 22980
cgttcaatca ttacctcatt ggaaagagat gagagttcca ctaaacggtg gtcaataact 23040
ttaaaattgt cttttactct catcttcatt ctctctttta aactttaatg atggctctta 23100
gcttgttctc aaggtctgca agagtaccat tattatgaat aatgtcacgc tcatattttg 23160
tagaaatccc attttctgaa acgtgtgatg aaatcttatc cacattgtct ctttttactt 23220
caatagtttg gtgtgcaaat ctactcagcc actcagcttc agagtcaaat cttaaatcac 23280
tgattaagac aaaaccatat tggtttctaa gtgagcaact ttcatagaat ctaaccattc 23340
ttttttcaag gtctttagac cagaacttgt cgcccattac tttacggaca acttcagtac 23400
cccaaatctg ttgaatttgt cttgatgaga atttatactt tttgctaaac cccagacgtg 23460
tcaatagagt cggtttagca accttcttaa gctccataat caatcgtccg gttaactctg 23520
acataagctt gaagtccata tggtaacctt catctctgaa ggtaaactcc atagcttctg 23580
taacttttga catcagttca ggatatgata agtcaaaaac ctgtggagtc tctttagttt 23640
caccatacag gtcattccaa gtcaggtcaa atatctttga tgcagatagt ttaaggttgt 23700
ctgcaaaggc cattacttca acattgtagc cgtactcatc ctctaagata ttcttcacaa 23760
tagaacatga ggtgtctttt ccagaacgtg cctttccagt aaatgcgata atattactca 23820
ttttaccccc attaattact aaaaagcccc caactaagga ggcttgtaag atttataaag 23880
acttagtgta gttgctcagc agactctttt tcagccatag ctgagagttg cttcacaaaa 23940
tcatcaccga agttaatgcg tagtttctct tcaatgattg aagcaccaat attcaccaaa 24000
acatcattga ttgcttgcat actaacacca ccagtcatct cctcaatgaa ctcaatactc 24060
acacccatca tctgtgaaag ctggattagt gcaacgatat tcatcatcgt agagatagct 24120
ggaatcatca gtgcaacttt cacctgaagt ggttctatat cagctacaga gtcattttca 24180
acagcttctt ccaaagactc tttagtttgc tggataatct ccttgattcg tgggtttagt 24240
tcttctacac cccaatcaag tttttcatat cgtgacaact tctcttccat tcgcttctca 24300
agagcagcca taacagcact agttgatgaa attaacaggt caattagttc atcattacct 24360
gcgagtagta gcaagtcttt ttcttcagtg gtcatggcaa tatagtcatt tttctgcatc 24420
tcataaaaaa tgaactctgc aagggcatca acacctacaa cgatagatgc taaagcaagt 24480
gttttgtcgc aaagcatcat gttgacctgt tctgcaaaag caccgttatc agacttgtct 24540
tcaggcaagt tatagcttgc aggcataaac tgttctgtgt agttgttacc acgaagaatc 24600
atagccatgc tttctacagc attgattaag aacgcttcgt caataccttt ttcttcaagc 24660
atcttttcta catcgatatt catattattt ctctctcagt tggttaagtt tctttctaga 24720
acattttaag ttttcggatg aagaaaagtc aagtgtcttt tcagaacgtg gataatagtg 24780
cctatcccaa gaagtttcaa catcgttaat cagtgacgct agattacaca ggtctgtacc 24840
actttgcaac ctctttttaa gagaatctag taaagcaact gtgtctttag ctttacgtct 24900
cgctgtagca acatctttca tatgctcaaa tacagagcat tttaagtcat cttcatagtt 24960
ttcagacaac tctatttcat gttgtatatc aaccattctc ctgtgatggt gagcatattc 25020
ccgttgagca gcaatgtcaa gttcttccaa cttctgcaaa gctctaacaa agtcagctat 25080
gtctgggtga acaaacattt tctgcatgta agcctctttt aagtaactat taagttttaa 25140
tcttttatct taacgtattc tatacgttat actttaaagc tttttaaaag ctattaaata 25200
atcttttaag agttttaaag tatctgtata gttaaactgt taagtaacct tttaagtaaa 25260
aacccacttc gcaaagatta acaccttgtc aagtgtattg tcaacttgct ttttataatt 25320
ttttgcagta atgtatgggg ttaatgattt tagagggagt aaaaatggaa aacgtagatt 25380
ttaaaaactt acatttggtt ggtgatacag aaactgatgg tttactcctt gagttcacta 25440
aagttcacgt aatggccttt gcagactata aatctgacga tgaagagcca cctgtatggg 25500
tctttacaga tgagcctatc ctcggtcaca agtacactaa gtacattaag ggtggtttac 25560
gtgaaggtgt tgagtttgcg ttaaaggcaa aacgactttg tatccataac ggcctcggtt 25620
atgactggtg ggtcttcaat cacattgcac cggatttatg gaactttgat aatccaaaat 25680
gtaagccgtg gagtaatttc tttcaggatt ctcttatcca gtctcgtgtt cagtggatgg 25740
atagaccaac tccaaagggt tataaaggtg ctcatggttt ggctgcatgg ggtgctcgtg 25800
ttggtgttcg taaaccagag attgagcatt ggggtgtgtg gaatgcagaa atcttcactc 25860
gtgttgtaga agatatccgt attaacgcca aaactaaacg ggcacttgat aatgagtatc 25920
tcaagcttaa gaagtgtggc atagacactt atgaaaccta catgcgagct aaagaaacat 25980
ctttctggat gagtcaacaa gctatcaatg gttggaaagc tgataaagag cttatggagt 26040
tccatgtgaa ggaacttgac aagttgacta atgagcttgc ttcagaggtt gaaccacatc 26100
ttcctccaac tattaagacc aaaggtaaag tcactggaga agagtttgca aaagcttgga 26160
atgagtatgt tgaaacattt ggtcatgcag atggactgaa gagaattacc aagtacccta 26220
aaacaaagta tcgtcagcag gtacgtaacg gtgagatgca aacttatgaa atcaagccat 26280
ttggtaagcc aactacaaag atttttaaca ttgaaaagag aaattgctat acaccaacca 26340
acactgtaac tggtgaagag tacaaggaag gttttgtagc aatgaaggat gctcgtgcaa 26400
tttgcaatga gttgaacgca aagattggta agaagtgcaa agactggaag ccagtaaaaa 26460
cagtcaaaac tgtgaagtac tataacagtc acgttgttaa ccactttgaa cttgagtcaa 26520
gtcgctacac aggtttgatt gatgcaccat atacaccaat tgagttcgaa gtttctcgta 26580
tgactcaggt agcagttgtt aaagactact tgaaatcagt tggttggatt ccagatgact 26640
ggaactacaa gaaagactca gacggtcgcc ctgtcaaagt ttgtcgtttc aaagacaata 26700
aaaagatgat tacaaaacat cctaagtggc aggaaatggt ggaacgatgt ggtttgagtt 26760
atgttgaaca tgaaggtgta cagtacattg agcataactg gtctgtgaag aaatatacag 26820
atttgcttga gccttgctta atccgtactt caccaaaact taccgaatca tcttatgata 26880
cgattgaagg tgagcttgga cagaagattg ctaagtacta tactttgatg caccgacgca 26940
gaactattga gaactcaaag gatgatgaaa aaggttggtt gaaccagatt cgtcctgatg 27000
gtcgccttag tgctggtgca atggtgtttg gtacttcaac tgggcgtatg acacaatatg 27060
gtattgtaaa cgtaccgtct ggtgctgctg tctatggagc acctatgagg gaggtgtgga 27120
tttgcgagga aggggctaat atcgtttcag ttgatatgaa ctcagcccag ctagttcttc 27180
tttgtaactt tatgggtgac aaagacttca ccaaagcggt aacacaaggt aaagaagaga 27240
ttgagttcat tcgtcaagaa gatggacgct attactgcaa acactttgat gagtacctca 27300
acccagagat tgataagtac cttcgctatg actctgagaa tgacctgtac gttgtctatt 27360
cagggactga tgcacataca ctgaacagta tctactttag cttgaacgat gagcaggaca 27420
tcttgacttg tcgagctact caggatgaga atcttcttca tgagattagc aaaggtcgta 27480
agaaagctaa gaatggcatt tatgcgtcag tggcgcatgt aaaacgttgt gaattgctgg 27540
aagcctaagt ctgtaaagat atggtaatca gcagcgaagt ctctgttagg actcctaaag 27600
caaataacat aactatagag attcctaaca gagaaacgtt cagagactat cgaaaagcat 27660
acttaatgta tgaactgagt agagtagggt ggtaagctaa tgaccattcc caagtgcaac 27720
ggtagtatta ctactacgtg atatagtccg acactccgta gaaatgcgga gaagttcata 27780
agagaactgg tacaccttgc gagtgtattg accagatgta ttgttcggtg caggtgatga 27840
gaagtttgct aagacgatta aagcatcaac aaccgaagaa ggtgctttaa ctaagcaaac 27900
ttactttgta cgcctcccta aaattaagaa gttgctagat gacttggaag ctgactacaa 27960
agcaactaaa aaggcacttg aagaggtgtt tggtaagact gctgcaatct ctaaaggggg 28020
ttttgtaaag gttgctggag cttggttgtg gtgtaaatct ccacataagt tattgaatta 28080
tttgctcatg ggttcggaaa gtcagattca gaacgaggca attaaccttg cttgtagaag 28140
agcatgtgaa gaagggttga caaaattaaa tggacggaaa ccagcaattg gtgctagact 28200
tttgctggca taccacgatg agaacagttg ggaatgccca gaaagtatga ctcaagaagt 28260
taaagccata actgactgga tgtatggtca ggcatctaag aatttaggtt tgaagagtga 28320
gacgcttgta actggcacag gtaaggttgg taagagctgg tatgaggtac attgatggtg 28380
ttgaaatgat tacagaaatt cgtcttaagg agctattgga ctacgacccc gaaatagggg 28440
tctttacttg gaagtttaga gaaggtaatg caaattttaa ctctagattt ggaggtaaga 28500
gggctggaaa ctacagaaca aatactgatg gacacaagtc agttcagttg ctgattgatg 28560
gaaggagtta ccaagcaaat cgtttagtta tcttgtatgt ggatggatat ttgcctgata 28620
aaaccttaca ggttgaccat aagaatgggg acggttggga caataggtac ttgaatttaa 28680
gggtttgtac agcttcacaa aactcttgca atcggtcaag aaagtcccaa tctgggttgc 28740
caaaaggtgt acagaagata aataaaaggt ttcgagcaat gatacgttta caaggtaaaa 28800
actatcatct tggtatgttt gacacacctg aagaagccca taaagcttac tgtgaagctg 28860
ctattaaact ccacggagag tttgcaaaac taagttgaca aggtgctcag ggaagagtac 28920
cttatactat atcttgtata ggagggctta tgagccaccg tggaagaacc catgcagcaa 28980
tgatgaaagg tgcttcaaag gaaactatca agaaccgtaa gcagaagttg tttgagagaa 29040
tgaacaggtt aattgacaat tcatctcttt caggttctga gaaggttttc ttgaaaggta 29100
atctaaaaag cattgcacaa gaacttattg acattgagta ttggagacat caaaaatgac 29160
acttgcagac gttattcagc aacttcacga caattgctat actccagagt tgattcagga 29220
gatgtccatt gtagtgatgc ctagtaagtt cgtaaaaggt tttaatggtg actctttaaa 29280
gtctgcgcac attcttattg ttgatggtaa gattgcaaga gaccgtactg gagtactcaa 29340
aggtgaacgt attgatattc tggagttgct atgaagaaaa tgtacagtct ctggggaagg 29400
gttggtaaag gttttgactg gacacttctt cgttcaaacg ttaaacgtag tgaattacca 29460
gaactcatca ctcactattt aaaaacatac agagaggtag actatcgtga acaataaggt 29520
taagagttgt gtgaaagcaa tggttgcact tggagtaatt tttctggctg gctgtaaccc 29580
atcttatgaa aacaaagacg cctcttatag cctccctcca gagatgcaag actgcaaaat 29640
ctacaaactc aatggtgacg ctataagtag ggatattgtt gttgtcagat gtccaaactc 29700
tcaaacaaca acatcttata gctacggcaa aaatggtcaa tcacatacta cggttattga 29760
gtgaggtttt cacgatggaa gtcctagtaa actatatcta ccgttatgat gttgttcact 29820
ccactacaac catagctcaa cgtaatccaa tcgtcccacg agaaggtgag ctggttcgca 29880
ttgatggttg gacttacact gtggaaagca tcattcataa gtttgatgtt gctggtgatg 29940
ttcaagttat cgacgtagag attggtggta agagaaaatg actgtagaag ataaatttaa 30000
gaacgcagtt cttacagaag atggtgagct tgaaaaattc attcttcgtg ttgatggtaa 30060
gctattccgg tgtcgttgtg ggtcaaactg tttccataaa ccagataaaa atgacttaga 30120
gctttacgca tgtaatgctt gcaatacttg gtatcactca gaggttaaaa atgacaatcc 30180
tatacaaaca aaataaagat ggttccttca acgtctggtc atgcgttgct gtaggtgaca 30240
aagttatcac aacctacggt aaagaaaatg gcaagatgat gtttgaagag tacacagcag 30300
agcctaagaa catcggtaaa aagaatgagc gtaacgctga acagcaagct ctctttgaag 30360
tttctgctaa gtacaagaaa caagtagacc gtaaaggtta cgcttacaca aaagagtctg 30420
cacagaatac tgagaaggta ggtgtacagc ttgctcatga tgctgcaaag gttagccatg 30480
caaagtatct gaagttccct gctgatgctc aaccaaaact tgatggtgtc cgttgtagaa 30540
tttcaagaga tgctgattca gtcggtttca cagcttattc tcgtgagaat actgtttaca 30600
acgttccagc agaactaatt ccagatttac ttttgttgct taaattacat ccacaagttg 30660
aagactttga tggagagatt tatgctcatg gttgggactt agaagatatt gtatctatga 30720
ttaagaatgc tgacaatcca gaccgtcacc ttcttaaatt ctactggtat gatatctgtg 30780
acaattcaaa atcttggcct gagcgtcgtg aagttattga taactcaccc attgttgggc 30840
ttggtgatac ctgtagagtt gttcctgtac agactatccg tgtaaattct tgggaagagt 30900
ttgatgaagc tcatgataag tgggttgaag ctaagtttga aggtgcaatg taccgttcaa 30960
tctctgaaga atctttctat gagtgtggtc accgttcata cttcttgatt aagcacaaga 31020
agatgcacac tgaagagttt aaagtgactg gtgtaaagac tgataagcgt ggtcatggta 31080
agttcgttgt agagactctt cctaacgtct ttgtagatgt ttcatggaaa actactcatg 31140
agaagaaaca gtatcttgct gagcatcctg aagaatttat cgggaaacct ttaacggttc 31200
agttccagaa gatgactcgt aaggggtctt tacagttccc tgttgggtta gttatacgag 31260
attatgaata aaaattgttg acatagaaaa tttggtgagt atactgagca gcataaacca 31320
acgggtactc accagcatca cttaagggtc ttctaagagg gtttttaagt gatgtttggt 31380
gtggtgcggt ggtgttgggt gtagttttgt gttgttaggt gttgttaggt gaggtatggg 31440
tggatggtag tagccacgtt aaaaatctac caagtttcta aagagtcttc tgttgagggt 31500
tctttacaaa caacttaaca atcaactaaa gtgtggagct aaatgctatg aaactgttaa 31560
atatcaaaat cactggcact cgtcctttct tgagccataa cgatacgttg tcagacccat 31620
taaaccctct gacaaaatat cacaagtctc tgtccagtaa acgtaaaaag actgacgaag 31680
actatgcgct cttagcagaa agtcaactgg taacttcttg ttactacgat gagcagttag 31740
gttttgttat gaacggtgag atgattgagg cttgtatcaa gtctggtgca aagctcaaca 31800
aacttggtaa ggtgattgac cgagccatta tgctgacaga tgtagtcttc ccaatgacta 31860
tcaagaactg cccagcaaac ccacaagaac ttgctaagaa tccagacttt atctatgcta 31920
agtctgttaa gattggtaca gcacgagtta tgagctaccg tccaatcttc cgtgactggt 31980
ctgtagagtt tggtttgatg ttcgatgaag agcaaatcac acgagaggaa cttctgatgg 32040
ttctggaaaa tgctggtaac ctttgtggtg taggtgattg gcgtccacgc tttggtcgtt 32100
tttctgtaga agttatttca gaaggtaacg tttaattatg aatacaaaag cattagcagc 32160
aaagtttagt tacggcgaca ctgtatcaca ttctgagatg gacaccttct taggtattgt 32220
gaagccaact tatcaaggcg acattgttaa gtacgaagat gaaatgaagg cttatgcgct 32280
tacacgtcta aaccgacttg agaagtttat tgaaaagctt cttaaagaag agaagattta 32340
cctagttgct tcaatgggtg ttggttatcg tgtggttgaa cctaagcatc aagcaactat 32400
tgcaaaacgc aaaatgtctg gtaagatagg tcgtgcttta aagcaagcca cccaagcaat 32460
cgagaatgtg aacactatgg cacttagtca tcaagaacgc tcacgactga ttgaacagca 32520
aaaccactta gctgctatga aagctaacat taacaagcaa cgcagaaaac ctttctaagt 32580
tgactaggta gcctcttcgg aggctccttt ttaaaaggtc tttaagaggg tcttttaata 32640
aggtactgta ctgcattgtg ctgtctggtg cggtaaggcg gggtgaggtc tggtatggta 32700
aggtaaagta tgggtagcta gtctcaacta cattaaaatg agacttacct ttttaaaagg 32760
tctttaaaag ggtcttttaa caaggtattg tatggtgtag tttggttagg tatggttagg 32820
tgtggtaagg taaggtatgg aaggatggca gtagccttat aaaaaatctg ccaaataagc 32880
tataaaatct gttgacaagt caggtgttat agcttatttt tatactcaat gtaatacaaa 32940
atttaatata actttgaggt cattatgaaa aaggttttat tagctgctgc aatggttatg 33000
gcaatgaatt taccagtcaa tgcaacagaa cttcccaatg tggacttatc aggtgttcca 33060
gaagacactt gccagattgt aaaaggtgtt gctctagcta atggtgagtt acttaaacca 33120
atctctgaag agtctttgac agagatgacc gataaggtga ctgactacca gtatcgtgtt 33180
cttgcagagt atttcctgca atctgcaaac attaaagaga aacaccatga tgatattgat 33240
gtacaggcta tgctcaacca tcgtatccag tttaaagaag atttgatgca aaaagctatg 33300
tatggtgttg aatatttctt agaaaacaga agctgcacag gtatttgata tggctcttaa 33360
aaagttacat cccagaagtg gttatggtaa gataattgac gatacagacg gctttacagt 33420
ctttacagtt atctgtcaag atgattcaca gattgaaaag gctcttgatg attatcttaa 33480
cgatgaacgt gaaaaggtta gggctacaaa catagattca ttgattgaca cttcacgcaa 33540
acggaagaag aaagatgaat gaagcttttg acccttatgc cccacaagat gattgggagg 33600
ctgatagaga ggctgaaatg gagagttata tttgtccaat ggatgtagac gaaatgagag 33660
acttcgttgc acatcgtttt aagagagaga ttaaatccag aggtttttct caagagcaag 33720
ttgctaaaat ttgtggtatc tctcaggctc gtgtatccaa cataatccac ctcactggga 33780
atgtctctct tgagtatatg ttggaagtgt gtgaaaaatt tggtgttaat tttaatttaa 33840
ggttggcaga ttaatatgaa acgtgaaaac attatccact atgagaactt cgcattaggc 33900
ttttacggtg tacctactca ccttgaaaag tattatggtg taaaaattct ttccaatctc 33960
attatggctt acaaagatgg taagattaag catactgaga agaaacgtgt catgggctat 34020
atggcggtag gttcagcaat ctcgaacatt aagttggaaa caactagcag tcagattgta 34080
aaagaccact tcatcaaaga gctttaccag aatcttgatg gtgtagatgt tcaggctgtt 34140
tggctggatg ttgatggtca taactacaca agttttgttt ttaaaaacga tgacatcaag 34200
tgtctgttcc cataataggt gattaactat gattgatatc tacttacaag atactcatgc 34260
agacttcctt aaagagatac ttaaaaagtt tatggcttca cagtatgaga atgaagcatc 34320
ttttaaaata gttacatgtg gcgatgaagc tggttttgtt gagattgaac atgaaggtac 34380
tggaaagact gtttgtaagc tacctgatag tatgttctct aacacgttct taacaaagac 34440
tagtataaat gttaagcttg tgcctcagat tgaaacatac tctggtacag attaccctaa 34500
aggttttaag tcactgatga agtacttctt agatgacttt gtgagtaatc ttctgaggga 34560
ggtaaaagaa agtcgtacag tgttgactgt agagaacatt ggaggtacta tcagggtcac 34620
ttctgactgt tttgttatga gcctcttcga cttcgttcct aagaactttg atggtattct 34680
ggatgaagaa gatgactgtg tagattttat attggttctt gagccagttt ttgaggttaa 34740
atagatgaaa attgaacact gctatgagtc tgatggaaca cctatccgat gtccacattg 34800
tgggtgtaca gatttacaag gtgaagttca tgtgattgtt aatggtcata tagctgaaga 34860
gagtacgaca tgtgttgatt gtggtgagca gctatcattc tgggcttatg gttcatacca 34920
acctacacca cacctaatat accatagcaa taaagctgtg aagaatgtca tcaactggtt 34980
cattaagaaa gggtttacaa aatgattaaa ttaatctttg caagtggtga aaatggggag 35040
tttggtactc caactggtat gccgtggcct cgacataagc aagatatgca agagtttaag 35100
agactcacta aagacaactt ggtagttatg ggtaatgaga cttttaagac tctgggtagt 35160
aaaccattac cagaacgtac aaacctcgtc ttaacgaact ctgttccata tttgggtata 35220
gactttgaca aagatgatgt aatgtatgct aaggccagta aagaatcctt tggagcattt 35280
ttgaagtatc ttgacagctc tattgatgaa gatgtctttg taattggtgg tgcaagtgtt 35340
cttgtcaatg ctttaccgta tgctggtgta gttttccata cagttttcca taaagtcact 35400
gaagaggcca ctgtgtattt accttttgaa aacttcttcg ataaactgta tgatagccgt 35460
gtatttgcaa aggtacagtc aagaccatca gatgatggta aagcaacctt tgaaatctat 35520
gttccacaag taaaaggaca cttttgatat gtcacaagct gattcaagtt acaaaaatat 35580
cctgaaccat gttttatccg ttggtgaact gcgtactaca cgaactggag atgttatctc 35640
tgcatttgct ccaccacagt ttcgctttga tatgcgtact ggtttcccac tcttaacatc 35700
taaacaggtg tttacacggc aagttattgg ggaagcttta tggttcctga atggtgagaa 35760
taaacttggt gaactccgtt accgtacttg gggtgaaaat gatggagaac gctggactat 35820
ctggtcagac gattttaaac gctggttaag ctctaactat tcctctgaac aagattggtt 35880
agaagatgca ggtgggagaa tctacggggt tcagtggaga aactttgaag gccataatgg 35940
ttgtgttgta gaccagttag agaccttagt aacgaagatg aagggtgata tcacagaccg 36000
ctacatgctt gttaatgctt ggaatgcagc agatattgca gcaaactcaa tggctttagc 36060
accttgtcat gttctgtttc agatttacat cactaacgag ggtgaagtgg atttgcaatg 36120
gtatcagcgt tctgtagaca cctttttagg acttccgttt aacattgcat cctatggttt 36180
tattctggaa gttctttgca agatgactgg atacactcca cgatacttga taggtgtctt 36240
tggggatact cagatttatc agaaccatat gaagcaggtt tatgaactga tgaacaataa 36300
agagtttcat gcacctactt ttgagattgg tgtgcagctt aacacactaa gtgacctaaa 36360
acaccttact gcaagtgatt ttattggtgg cattaacaac taccaacatg caggaaagat 36420
tgaagcacct ttgtcagtag gtaaataaaa aacaaaaaag gctcccgtaa aaaggagcct 36480
taaattttac ttttcagtat tctttgtgtt cttttcagta atagcttgca aggctgatac 36540
tgattgagcc agattattca cactgtcaga gaatttatca agggttttgg taagttttgc 36600
gttttcaccc ttaacattct ctaactgaac tttctggttc tccatcccta gctgaatcag 36660
tctcatgtca gactgtaaat cacgaatagc tgaatagtta cttttggaat aattatctag 36720
ctgctgtaac tttgttgtga cagacacttc ttgcttacca cttgaaactt gcatcgtggt 36780
atacatccca ataacactaa aaataccaac aacaattgca ccaatattat ttttaaaagc 36840
ttcctctagc cacttcattt atttctcccc cttaaaagct ttttctaagt tatctacgaa 36900
ctcatcatca ataggtgtgt ctgtcttacc cgcaaggtat cttgcaagct taaagaacac 36960
tttctcaatc atgtattcac ttagaaggga taaaatgagt ttccagaaga agctacctag 37020
attttttagg agaattgcta ggattgtagg catttaatca cctcatcagc caagatggtg 37080
agaataccca taaagaatat taacaccatc ttaacaatca gtcaataagg gattaagcag 37140
ttcttaccca agccatcagc ttatagaact ggttagttac actaaatgct gaaccagaac 37200
ctgtaccacc agtgttacca ctaactgtat gactgtgagc accaatacca actgtgtgag 37260
catggtttcc gtcagaggca gcagtaccag atacagtgtg tgagtggtca ccagctacac 37320
tggaaacctg tactgcacca aatacctgaa cacgcggttt accaccgata gagtcaccac 37380
cataatgacc accaactgta tgtgtatgag caccagtgtt gttagtagaa ccactcactg 37440
agtgggtgtg agcaccagta gtgttagtgg ttttcgtacc atagtcaaat gatgaagtag 37500
tcgcagagaa gctatgagtg tgtgaaggta agttaccaac agataacgtt acagaatctg 37560
aaccaccagt tgtagcaaca tctgaaccgt ttgctgctgc aattctaata gttctaccaa 37620
caccattgtt cagatacgtc caagttaacc cagggagtgc tgtattaggg ttaacattac 37680
tgttaaacca tgtcacaata cctactggat agattttatt caggtctgta gagtcactaa 37740
tcgcttctgc aatcttctgg tcagtttctg atttagtata tgcaccaata tctgaaggtg 37800
ttggcttcaa gagactagtg taatagtgag caattgtccc atcaacatag ccagatggag 37860
cgttgtcaga tgtctctgga gcctcaaaca gttgtactgt tgcattagat gttgactgaa 37920
ccctagctgt tgtagcgact agatagttac caactgcgca ataaatgtca tagttatcac 37980
cagaggtatt aacataccca attctactta catcattatt cggtgagttt ttccatgcaa 38040
caacatttaa gccttttggg ttaccatccc ctgtcctaag aacaatttct gtcttacagg 38100
cttggtcaat taggccaaag ttaaatccag ccccaccaaa cagttcaatc actgctgttg 38160
atgcgccttg tggcatattc acagttgcaa tcttagccca tttaccagca ccactagtat 38220
tgaaatctgt gcttcttact ctcagatatc tgttatcagc agatgatttg ttgtagtatc 38280
tcgcatcaat gttagaccaa tctgaaggtt gaacctgccc agtgattgct aaagttgcat 38340
tcatttcgaa tgttgagcca atggtaagca ctttacctgt tttgtagtta aacagcctta 38400
agttattatt gttatcggca tcaccattac caacatacca cttagctgtc ccatcagcag 38460
cccaccccct aatgaacaat gtcgtttcag tttttgcttt taacgcaaga gcttcaccat 38520
cagagttgat aacttgtcgt acagaaaatg tattgagagt attagttctt gcaattgtac 38580
ttaatgttgc tgccggaata tatctagagt caatgttagt ccaatcagat ggttgaactt 38640
gaccattgat ttgcaaggtt ttattaatac caacagtagt acctagtgaa acctgtgaac 38700
ctgtaatgta gttataaata ttcagacgtg tctcatcaga atctgtacca acataccatt 38760
tatttgttcc atcagacttc ttaccaagaa tataaagtgc tgtacctgca tcagtattct 38820
gcaaagtaat agcattagca ttcttcttaa caacaaggtt agtgaaagta ttagcacctg 38880
taaaagtgtt attaacagct aactgtgcaa atctctgatt agctgctgtc tgagtaaagt 38940
atctagcatc taagttagag aagtcagaag gttgtacttg accagtgatt gctagagact 39000
tattcacaga gatattgctt gcaatagtca aagctgagtc atatttgtta ttgtacaact 39060
gcactgcatc agaaccattt ttgctaatac caaccaacca tctagccgag ccatctaggt 39120
ctacaccttc aatatattgt gcattatttg cattttggtt tctaaggcga agtaagttaa 39180
catcaccttg aatgttctga gtcccagaga atgtgtttgt aacagcaagt cttgcatatc 39240
ttgcgtcatt ctcttcatta gttctcatac caagctcatt aggacttggc ttattcagag 39300
tatgataaac agttgctgac cttgaagcat ctgcaatagt taatttaaga ccatttgcgt 39360
taattttaaa agtctttaaa atatccccca tagtaatctc agaagagcct gtagggtcaa 39420
agactgcctt accaccataa agtaacttaa caatccctaa gtcaccagtc atagtactac 39480
cagcaatctg aacaaatctt tcaagcttaa aagatgctaa gaagtcttga taggtcattc 39540
gacggtcttc atcacccagc atttcaggtc tctttttaac cctgacatga agaaggtcgt 39600
ctgaacggat tgtatcgatt gattttaatt cactcaattt gtaatctgcc attatttttg 39660
tcctctagaa agagggctgt acagccccct gtaaaagaat ttttaagcgg ttctttgcca 39720
tacataaacc acaaaggatg gttgttcaat gctaaatgct tgaccagcac ctgtagtacc 39780
tgtagtacct gagtgagtat gctcagatga ctgcaaagtt actgtgcctt tatgggtatg 39840
agcaccaatt gcaacagtat gggtatgagc accaattgca acagtgtgtt ggtgattacc 39900
tgtggtattt gttgaaccgc taatagcgtg tgagtggtca ccagctacac tggaaacctg 39960
ttcagtccct gagacctgaa cacgatgttt accaccgata gagtcaccac cataacgacc 40020
acctacagta tgtgtgtgat tacctgtggt atttgttgaa ccactcactg agtgggtgtg 40080
agcaccagtg ctattagtgg tctttgttcc ataatcgaaa ctgtttgtgc tcttattgcc 40140
gtaatcaaag ccatcaatag taatagcagc accatgagtg tgtccaccac cagttaggta 40200
gattgaatgg ctatgtgatg gtaggttgtt actagataag ctaacactac ttgagccaaa 40260
gttactacca actggcctag aatcactatc ataacctaca agtgctcttc ctcttgaaac 40320
taactcccaa gtacctccac aaattaaata tgtagaaggg tttgcaggat tcatagagag 40380
atggatagta cctactggat aagaagcctg aacagccttg tacaagttat ttactgctct 40440
tgctgtagca tacttatctg catcctcatt ataaaggtta gatgtcaccc agttctggac 40500
attgctcaaa ccaacctgtg ccttagttgt attgtgtggg ttacttttat cattaatatg 40560
ctgttgaaca aggctattaa cctcttctga agacataatc tgtagatttg ctcttgcctc 40620
agtgacatta gcaacatctg ataagttatt tgcagcaact aactgaagag cattgataac 40680
attatctaaa ccaatttgtg ttttagtaac accgtgaggg ttatttctta ggcttgagtg 40740
tggagcaagt aattgctcaa gagtacatct cttatcctca ataccctgct taagatggaa 40800
gatatcacta aggtcaattg gtaaggctgc ttgaggcaag gcactaattt gaatttcacc 40860
tactgccatt attaagctcc tacaaattca taagtgtaaa ggttttttgt acttgttgat 40920
gcagtacctg tttcggtctt aataagactc cagccattag ctactaaatc tggttgctca 40980
gtggcaaaag accttacttc accaacagtt ccgtttgctt tcttcaggag atagtttaag 41040
atatagttaa accattggcg tcccataggc tcacccctta ataaaccagt ggcctgaatt 41100
tctggtggtg gtaatacttt tagctggtta ccatcagcat ctacttcatc tgtagaccaa 41160
tttaaaaatg ccattaagaa cttccttctt gctgtgattt atcttttcta cctttgatga 41220
tttgagctac ttcagccata acaccataat caccacctgc tacagtttct ttaccaacga 41280
tgtagttatc tgtagcgtta taatttttat taacttttaa gtaatctact gagccactat 41340
tagccgtcct atcaacctcg aaataagcat ctcttacgcc agcatctgct aagctaccta 41400
gtaagttctt atctaaagaa ccacctgtgt tgttagtaac aagacccttg ttagcatctg 41460
taacgaacca gttatcttta tcatcaacaa ttgctaaggc tgagtcagcc acctctacag 41520
gtgtccaagc agtaccattc agagttacgt ctcttagaat aactgctgaa ccaatggttg 41580
ttgcagcaat ttttgctaat gtgtacgctg tatcaatgac gttatttctt gtattaaccc 41640
tgactacaat accagcagtc acaggtgtaa tatgctcaaa aatctgtgaa aatgttgcgt 41700
tatacagagt catgatagca ttctgtaaaa actttggagt tgtgtcagaa cgtcttagaa 41760
aaatttggat atacagcatt gctctgtatg tttcatcatc agcaccaagt ggtcgtggta 41820
ctttaattaa tgaaccaatg ttgtcaagtt gctgtccaac agcttttctg atattccttt 41880
cagtgtgcat ttgccatgaa acatcttcca atgtctgcaa ctcatcagta atcgctttaa 41940
gtaagcttgt atagatgaat ttatctttaa actgtgttac agtcctttca tcgagagtct 42000
tatagtaaac gtcgtcaatt ttctgaaaca ttattactcc ttagcaatgg tgtactgact 42060
actttcccat acagtatatt ggtcaccatc aacagtaatt cttgccgtag tatactgccc 42120
atcactagga ggtactgatt ggttatttga aagagctact ttgatttcat taatctcaac 42180
acctttaatg acatcataaa tatacccata gattctgtta gggataacat cattaccaac 42240
tttcaaagtt ctaccgtaag cgttaatacc ttgaacaata ctatctctga tatcttcttc 42300
tgggattgtt aagctctctt catcatataa agaataagat actttgacaa aagcatactt 42360
gggtgttggt ctgctaaaat agacattatg agctaagtta cctaagtcat aagctgtacc 42420
aaagatagct ccataagccc tgataccagc aggtttggtg tcccagattg cttgagcaac 42480
attatcattt tgaccaccaa ctacaacaat cttgaaagac tttggtggaa gcccttctga 42540
acttgtctct tcagtatcat tttccacacc tgaagcatct gatacaccct gaactctttt 42600
aacagcagct acgattgcat caagagtacc tacaccagta actgccaaag attctaaata 42660
tctctgtcga agttctgtat cagtttcttc atttctacct gttgtcaggt catatctgtt 42720
gtatacactg tcaagaccat ctacagttgt ttcaatttcg ataagtgttc cagctaatgc 42780
agggattgca ccaacttcct cagcaacaac atcattgata gttgtaattt ttgtgaatgt 42840
aaggaacgtc gtagcagtca ccaccatagg gttggttctg gcaataatgt caccttcatc 42900
tttataaact cgtaatgctg aaccgtcatt gataacttcg gcttttgcta cgataccacc 42960
attgattgca tcggcaagtt cagttaatag tactgtgatt gtatctgaag attttggctg 43020
ataagagaaa ataacgttat caataataat aacataatct gcatcagttc gtaaagagtt 43080
aacttcaaga acagcttcaa cacaatatga aggtgttaat gtaatgtttg aaactggata 43140
gaagatatta ccagcagtgc ttcttagtct agttgtagat ggaattgttg caccagttgt 43200
tccagtaaac tctacttggc ctcttgtagc ctgagccaca tatctgtata cggcgtttaa 43260
agctgtaata tcatcgaggt taaaaccttc cgctttatca atcgtcccac catcataaat 43320
ttctgacagg acttcatggg tgtctgctaa agaccttgca attgaagcca gaaagagacc 43380
taattgacta tcttcagaaa cgtcaaggtt tggtgaaata tctctaagaa gtcttgattt 43440
aatattatca aaaatttcct gatatcttag agtttgtaat cctgttgtag ttaatcctgc 43500
cattagatat taacctcttg cgtaatgtct gttaaaatat ctgttgtagt agttgcatca 43560
aaattaacag ttacttttct ttgagcattg tccattgaag acgagtagtt atagatattg 43620
gatacatctc ttgtttcaac aaggtaagct ttcatataat tatcaaagat agaagttttc 43680
tgtttaaatt tggcaagttg taaatatggg aatccagcag atgtgttaaa gaagacttca 43740
ccagccctta aaaggcatct gatatgaagt ctttgagcaa cctgagtagc tttatcatct 43800
tctgggataa ttctaatttg gttaccagta atctttaaat caccatgagc cacatacact 43860
gaatctgaac ctaaagtggc aacatagtca ccacctagat ttaatgcaaa atctgttttc 43920
attattgtgc ctctgtagta tcagcctcac cagcagggtc tgtccagtaa taatggtgcg 43980
tgtgttcatt aaagctcaca ccagtcgtgt cactgataaa atctgaacca tgtacttctt 44040
ctgttacgta caagttttta gaaatgtgta catcaccttc aaaataaaaa ttgccatcat 44100
ctgtaactct taacacagag tcaccgaaat gaagtctgac tgctgttggg tctggtttaa 44160
aattctgtgt tcttgtgcag atgcctacga aagctacaca gtctgagata tcgtgtgtcc 44220
ttctcatatt tgtttccatc tgagtattct tgtcattgac aacgaagtca tctaaaggta 44280
acattgagaa agctagccag catctgtcat tagtttttac agggaatgtt aaagatgcgc 44340
caccaccact tggaaattga acaggaacac cagtaatctc tggcataggt aaaccgttaa 44400
tagagtaaag tggcttaaca gtggctgttt gagtctttga atcaaaagac tggataatag 44460
ctggtaagcc agtatacagt tcttttctaa attcatcaag acattctgaa acatacccag 44520
acattctagt aactggtgac ttcattattc caccttctct aaatctagtt cagttgtcca 44580
agcaccacca gtaaagtcaa gattatgaga aagacctttt actcgatact gaccttcaaa 44640
atcttcactt tctctaattg tgacactatc acccatctta attctcccgt ctaaatggat 44700
tttgcaacga actccagttt taactttaat aactgtctta ttctcttttt tcagaacctt 44760
tctagttctt ctgtagtaac cttgcaaaga atcaataaca ttatacgggt aaatttccca 44820
agaaagttgt ctagccttag cgttaaaagg gactactctg atttgcttgt taaatgtata 44880
ccaacgtaga ctactttctt cacaaacctt tgtaagtgcc tctgcaacac ttccccaaac 44940
actaaaacca ttcttgtaag tgtaaccatc aatacttgaa aggtcttcat caataagtga 45000
gaagcctaat ctattgacta aatctctaat tacactctta cgtgttgtac ctgctttata 45060
agagattgat gtcttaatcg tggttctttc cattttatca ttggaacaga tgacctttgt 45120
aaccatatca acaccacgct tatatgtata agcatattca atagttccta gataaattaa 45180
tgggaggttg tcatactcaa taataaggtc accatttgcg tctcttttaa agccagtggt 45240
gtaacctgct ctgagcataa ctgttgcacc aactgtttta aatttggctc tcatctcttt 45300
atttaggttg tagatttcaa aagtggtgtc atcggaggtt actttatttt tctgagacgt 45360
ataagacacg ttacaagtga attgtaagtt gtcgaaatag tccatttgca tagaatcttt 45420
ggcatggctt gtaggtttat cattaaaggc tgtagtttca ctacctacag ccaattgata 45480
gcacctaaaa gaagccccag cagtgctatc ttttacagac attataaatt ctccattaat 45540
ctcatatctt cttgagtgta ataattaagc tcaaatgccc tttctcttcc gaaattattt 45600
ctggtaggtt gtaaatcagt accatacatt cgttcaacaa aaagctctcc agctaatgaa 45660
ggaattacat agcgtcctgt gattgactgg tctgcaaggc atttcttttc agataataat 45720
acattaccat caacatcaga tagcgtcaag aaccatctgt caagcctctc tttatacttt 45780
aactcaatta taaaaacagt accatccaga gttacagttt gtgtagacca ctctgtatca 45840
ggaacaggaa tatattgtga cattaataag tccccttctt attcggattc actgagtgct 45900
tttgtaatgc cttaccagtt ccagcagtcc tatttaatgc tgctccggca tttttttcag 45960
cctcatcact gaatgtggta acaccttttc ttgtcttcgc agacattgag catttcccaa 46020
gcgcattgtc ttcagcacta gtgagttccc ttacaccatt agcatctagg tcaaacagta 46080
actggcaatt cagtttacca ttacctaaac ttgttgtagt gttacccgta tttttcttag 46140
aagttgcgcc accatcattt gtggtagcgg tctttccagt agctgctgaa acatcagttt 46200
gccctacaat agctttgaag ttaatttcct gaaaagttag ctggattctt agtccattag 46260
aaattccaac atctttagag gctttaaagc ttgtaataat ggaatcgtca atcttgatac 46320
cgtctttaca gatgaccgaa ataatttgtt tctggtcacg ccagttttca agagtgtcaa 46380
tgaagttttc tactaattgt ccctgacgag ttaataataa gctaccttca tagccaacta 46440
caacgacacc actaatagtg attgttctgg gtgctctttg cacattatct gtgacggttt 46500
gccctgattg catgttctgt gtagttacct gcatagggct gtcaaattcc atattttcag 46560
ttgctgataa ggttaagaag gcatctacat tatctcttaa gtggaaatag atgccatctt 46620
tgccactata tttgatttgc atattagaat cccataacat tattcttcct ctggatagct 46680
tgaacttaga agaatgtctt cttgattctt gtcagtaata tccaccatct tagtagcaat 46740
ttgtttacca tcaagattga aagtaacatt cagggtttgt ttagtctgca taggtaaacc 46800
agaaggtgtc atcatcattg gtgtctggtt aaacttattg gcaaaattat caatagatgt 46860
tgatagcttc tccatgataa tctcccaatt agatagacca ttatcaatta acttcctgtt 46920
accttcaacg tcttgagtgt actgtgcaaa ctgaagttga ccattctcat caaagaacat 46980
tggtcttttt gggtttgtaa tattagcaac agcattttca aatggttttg gtaatgtgac 47040
ttcactgtag tttttagcag catttgggtc tgtagaacct cttagcatta atgccgaacc 47100
aacagtgcca agtgccattc tcgttgctgt aactccacct gctgctgcgg ctgcttctcc 47160
ggctgctgct gtacctgctg cactaacacc gagtctttgt aagattttac caaagatacc 47220
cccaccaacc aaaccactta ggagcttgac tgcttttgat actacagcaa ctgcgccgcc 47280
aattgtgaca actgttccta gaaattcacc agcacttttg ataagctttt gctggctgtt 47340
gtcaaggtct ttataccaag ctcttgcata gtagtatagt gcagatgttc tgtacatgaa 47400
gtctgtaacg aagtcaagta gattactagc accttttaag agactcccaa tcacaagacc 47460
caaagcttgt gtgctaccca tagaaccttg taagaacatt gcaacagagt tagataactg 47520
tgaaataccc tcactagagt tgttaaacag tgctacaagt gtgttatccc acattgcctt 47580
agcttgaccc attgatgtag cggtctgctt agatacagcg ttcataccac ctgcttgctt 47640
gacaagttca gccattcttt cagatacttt aggaagaacg tcttgagcaa gcagtttacc 47700
atcttgcatc atcttatcaa gttcttgtgg ggtcttacca atggcatcag cgaatagctg 47760
tacagcacct gctaaacggt cacctaactg tccacggagt tcttcagcct gaactttacc 47820
ttttgatgcc atctgctgga atgcaaccat gatacctttc aagtcttcat cagtagcacc 47880
cctgatacgg gcaaacattg cagcattctt atagaactct tgagtacctt gaaaaccaag 47940
tgttggttga gcaccagcag caaagtttga gtactgcttc atagtgtctg tgtagttctg 48000
accaatctgg tgtgcgaatg atgcagcgaa cattctagct tgctgtgtat ctgctccaaa 48060
gatagctgta gaggctaact gtgcagattg tctttttaca ccagcttcaa tagtcttttg 48120
agatagttcc agtaaagcgt aagctgaaac aaatccacca actaattggc gtaatgatgc 48180
gttagctcta tcctgtagcc aagctgattc tttaactgat tttaatctag cattttctgc 48240
aataacccaa cgtttggtta cgtcgatgag ctttttaact tccatctcat actcaccaac 48300
cttaccagtc cctttgtatc tgttgtagat attttgcaag ctccctctga aagaggctgc 48360
catctggtta ccttgaccac caattgtttc cagtctacgg gttaaccctg aatagaagtt 48420
gttattaaac atcctttcca tttgtctctg agcaacatct actctctgac ctctgggtgc 48480
tccaccacca acagggggga tgctctgtcc acctctccct ctgcctgttt taatagtgat 48540
tttaccgtca acattcatag catctcttaa ggacttgtta ataccttttg cagtcttttt 48600
tgcttgagtt tcaagtttct taagagattt aacaccttgt gaatcaaggt tcaaggaact 48660
gttgagtgct ttattgattc tgcccgaagc agactgagca ttttttacaa ttctattaag 48720
tgcttcctga gagcttttat taggtttcac atcaaaggct ttattaatat ttctctcaat 48780
acgttgagca gcttgcatag acatcttctc aactctttgc aagcccttaa taaccttttc 48840
actgaaacca agttccacaa tgaagctatc aactgtatat tgtgccatta catttttcct 48900
gctcttctaa gttcgttgta agcaatttcc tctttatacg acctctgaat ttcaagaaat 48960
tgtctcaatg ataataaatc agagaatgtc atagcaaaga gttggtcaag tgtttcttta 49020
cacccttcca taccataaat agcaagcaca aatttcatct cgtctgcttc ttcataggtt 49080
gcctctacag cagcatcagt tagtggtgtc tgtagagtgt tgcccatgtt tactgagaag 49140
ttaggctttt gaaaatgctt gcttcgaaaa aacttccgaa gtttgcctcc agcgcaaatg 49200
ctaagtaatc aatgaactca ccatagtttg cttggaagta cgtatcaata ttaagtggga 49260
agtcatcaac agttgcccct tgaaataaca gagttgccat ttcttcaagg ttaatctctt 49320
caattctgtc aaaacaagct tcaacaagtt ctttaaatgg aaccattgga gcttccttct 49380
tatccttgtc agtcagactt gatagcatct gtgcaaaggt tggaacaaca attttaccca 49440
acttcataga catcttaata ccatctcttg ccccaagcag aacgatattt actttcttac 49500
cattaattac tctaaattct gttttcattg tgattcctta atacttttaa aagaaacaaa 49560
aaaggggaag accttttaaa gtctccccct tataggattt attaaacact tgacgctgga 49620
attgtagaag tatagtctag cttctcacaa ccaaaaatcc aagttttaga gttctggtca 49680
cgaccaagtt caatctgtgg caactcctgc aaccaagcat taataccagt tgccagaaca 49740
gagcctgatg ggtcgtagat tacgaagtta gaagagatat ctacttcaag ttccatattg 49800
tcttgtttag cttgaattgc agaaagcatc tggttagaga gagaagtctg cattagctca 49860
atctcaatag tacctgtctt gtctgcattt cttgtcagag caacctgacc acctgcacca 49920
acaactggtg tcacaagtgg tgatgttctc tgtagacgca agaatgagtc tggggcaaag 49980
ccttcaatgg caataccatt ccagctacat acaacgtctt taggggaata ttgctgatac 50040
atagccattc caatttacct ctattattcg taagccactg tacctttcaa gtcaacatcc 50100
aagatagccc ctgctaagat acctgcgaag gtaacatctt tcaggatacg agctttcttg 50160
tctgctaaag caacttgaga ggctttagga acattaactg tgtaagatga caggaagttt 50220
ctgttgactg ctctttgcag agaggtttca atgacttgac gaatacgggt aataccagta 50280
tcatcataag taatcttacc acccttctgg ttaattagca agtctctcag agaagttttc 50340
aggtctgatt ctaaccagtc aacaccacgg acgatatcaa tccattcccc accagaagta 50400
atccctctac gaaccactgg aacaccacca tcaaggtcaa taaagttaca gtgacgtaca 50460
tctaaagcag acttctgaat acttgtcaga ggtctctgat tagatggttg tagagaagct 50520
gctacaccag ttagctgagc attaccccaa gcaattgaac ctgcatcata tggagcacca 50580
taagcaatgt atgccatctc tgggtaatct tctgctgctg cgtgatgcca caagcaaact 50640
gtacgagtgt acatattctt agcaagctgt gctggaacat catttgcact ggctaattct 50700
gtaccttgca gggctgttac atcggagtta gctgtaaaga agattttctt gcgagcctga 50760
atctcagaag ccattgctaa gacaaactgt tgagttctgt cttctgctgc aatgaaatac 50820
cagtcggtag agtaagcttc gatagctgcc agagcagttg atgctgtatc agcagttgta 50880
cttgcaatat atacagtctg tgctgtggtt gttactttca caaagtcatt atcaccagct 50940
ttggcaataa tcattgtgac agagccatta ctaccagtta cattcacaga aaccttatct 51000
ttgattgttg ggtcagcttc aatctgtgtt ttaaactgtt gtaacacgtt ctcagcagtg 51060
tctgaacttt gtgctgtgta ctggtatggt tgagaaattc caccgccagc agctacagta 51120
attgagtagt ctgtactttc ggtaacggca tcaggaattg atacagtgta ctgcatagcg 51180
cgtctaccga tataaagctg agttacttta ggagtctgac tccaaagttg tttagcagcc 51240
ttatatgcag cagtattctc atcgaaatct tcagcaactt cagttaagga agtgtaacca 51300
cgcactcttt cttcaaagtt atctgttgaa gctaagaata gtggcaaacc aaaaccttct 51360
cttgtagttc ctgcggtgtt caatgtaata tctacattaa caattggatt ccacatttat 51420
tttacccctt tggagtctac atctagatgg atagtatatt ctggtggctc ttgtccttct 51480
tcataaacca actcaccatc aacaatgaca cgctcaataa tacttccacg ttcatccttc 51540
aggactgagt tttttacaag agttacaaca agaggcgcag aattttcgaa atctgtattg 51600
agataagtat agtcattcgg gatagctcca gtgtctagta ctgtagctcc tgtctcttca 51660
aggattaaat ctctgactga actcatctct aatcgttgtt taagctcaag cataatactg 51720
tgagcacctt taccattcac cgtaattaat actggaatct gaaaagcaat tctgtagcaa 51780
actacatcat cctcaacaaa cttatcaaga acccaaccat aaggtgttgc agcatcttga 51840
caatatacag tgataaatgg ctggtcaggt tttaaacctt tgtcatttga gttatcagaa 51900
gggtaagctc taattacgtt tggcctatta tttttatcac gagctaatct gtgaccaata 51960
acatccacta aggttctaac tagacctttt tcaagttctg ctgtttctaa ctgcattcat 52020
tttatccctt ctaataatga tatattcata atgggacgta tgggctaatt gctgtgacca 52080
atccatagtc ataaatactt catactcatg accatcaatc ataacaatat cagactcatt 52140
ccactctaca tcatctgaag ttctaagttt atatgtagta tacaggattc ttgtatcggt 52200
aagtctgata ccttccggta aagcaatctg tgtaccattc tttacagaac ctttgatata 52260
tggctggata ttacctttac agttaacctc tacaatatct tgtgaagcta cccaatcacc 52320
atcatcatta taataaccgt cttcagagac tttacgcttt actacaaagc tgtgtctgtt 52380
taagagtctc atttcttaat acccttctta gtagaaattt tataagcaag gttatctctt 52440
aagtcacctg tctcaacaag aggtgcgtta aagccttttt tcttgactgt ggaaggtgcg 52500
ttaggaggga gtatagcaga gttaccaaaa cctcttttaa ttgccttttg agcattcttt 52560
gcaaatgctt ctaaggtatt tgaagggtct gtgttgagac tgctaagttg cttatataga 52620
ttcttcttag tctgctctaa caaggtctgt ttgtttagca tcatagtgat ttcaaacaac 52680
ctacgatata ctttaccaga agctgaggga accccaataa cttcttgtaa atacattaaa 52740
gcaggataag aaaaaccaga gctatgttga ccttgttctt gaaaataccc aacttgagca 52800
ttagccgttt gcaagttctt catagccccg actaattttg ctctagcggg gtgaataacc 52860
cttttaacca ttattcatct cgctcgataa taaatacgcc attaacacgg ttgcagtaat 52920
ctctgccttc atatctggca gcatcaccat attctgtgta tttcttgaca gagcaaggat 52980
tctgacgacg catgtcaata tcacactgat taataccacc tgcatagggt agtcctgaaa 53040
cagagctttt aacaaggtca tcataaacag ctttcagaga cttgaatctg gaagagttac 53100
gtaaataaac accaccaacc ttctcatctc ccatcttggc tacttgaaag agtaaatatt 53160
taagagcttt gatggctgct ttcttttcat cctttccaga ttctagatag aaccactcta 53220
gcacagactg ctcaataaga atttcatcat tattagtgtc tgtgcagagg attcttactc 53280
tatcaagagg gttattggct gggtcgcctg tatagcacat tcataaccct cctaagttta 53340
ttagccacct tgagcgtcag cgcgaacatc taccagcaac tgaggacgag tacagtatgg 53400
cagcatgtaa gagtgagctt cgaagtcaat accttcgtca cggtcttttt cgtattcaaa 53460
tacgtacagt tcctgaccaa gtgtatttgc gtaacccatc ttaggacatg gaccatatgc 53520
cacttcgaag atgttgttag cttcacccag catagatacg ttagggaagg catgaccaac 53580
accaacagtg tctgctacgc tgtcaatact caccagagtg tgaaccttac cgcgcttgtc 53640
tttgaactta ccgttgtact ggacaaactt aacaccaccg tagtagaaag tgttcatatg 53700
agcctgaacg ccgtcagcac caccagttct cagagaacca gtaatctgtt gccaagccag 53760
tggagtctgc tgtgcaagat aagcgtcacg aatcttagga tgcttaacca gtttgctgaa 53820
gaatacacgg tcaacaacta cgtggatttc ttcaccgttg attacagtgc cagtcttagc 53880
ttcgtcttcc atgtgcatac gcagttcttc aatagaagcg tcgatgtcag cattagggtt 53940
gtcaaggtcg aagtaaatag tcttcttctc aacgtcgaac tgcttgtaca ggtcagcata 54000
cagagtacca cgagcatcaa caactttacc cttcagagct tgcataaaca ggaactcacg 54060
agtaatatcg aacttggtac gaatcttcat cagcttctta gcacgtacta cagcttcagt 54120
agtcagttca tttgcagtgc ctggctgacg tacaccctga atctcatcag gagtaatgct 54180
ttcaacttct ttgaagtaca tcattgggaa gctgatttga cgaacacgct caggtgcgct 54240
agtctctgct ttacggctat cacggtctac cgcatcaagc aagctaacat cccaatcagt 54300
caagtccatc aggaaagtag tttgggtgat tggtgctgaa cggaacagac ctaagttgga 54360
aatataccca taagtatttg ggatagactg gacttcacca gtcaggtcag caaggaaaaa 54420
tctgcttttt tcagaattag tcaacattgt aaaattctcc agaatgtctt attattgtta 54480
ttacaggcca gttggtacga aatcaatacc tttggcagcc agagctttct tgactgcggc 54540
agcgtcaacg cttgcttcaa gagtcagcat gtctttcagt tctgcatcac ggtggatacc 54600
aacaactttc aactgaccat gataagacag ttgcaggtct gcataaaagt taacgataca 54660
tactgaatca gcctgagctt cttgccctgc tgtaacctta gtaccatctg ctttcagaac 54720
ttcacctaca cggtattctg ttgaagcaac cggagtgtac tctttgcgag agtgacctgt 54780
tggggtaacc tgttcccaaa gaatgatatc attcagaggt tctctgttac ctaacttagt 54840
aaaaccttga tatgccatta ttgtgttcct tatttgataa gagattttag agcattctgg 54900
agagccagtt tgcgttgttc agcggtgtct tcggaagcat tcttagctgg ttctttttct 54960
tcttcaacca aatcagcttc gccgtcatta cccacttctt ccatagcatt ggaatgttca 55020
agaacagcac cagcagtttc tttcagcttg gtaatttctg attctttttc ttccattgca 55080
gaagcatggg acgcgatagt ttcattaagt ttctggttag caccttccat agcattcata 55140
aacagaacac tcagagggtt atcaagacca gcacctaaaa tagtcgttgc agcttctttt 55200
gcatcaaatc caaaagcttc agcagaagca gaaatcttat tagtcaaatc tgacaaagca 55260
gcttcctgtt ctttagcttt catttgagca acctgaagac gcaaagcttc tagttcttgc 55320
ttttcttgtt cagtcatttc ttcacctgaa ttgttaacgt ttaaacttac aggagcctct 55380
tcagaacctt gtaagtaatt taagaaatca tcttgagaca tgattgagtt aattaaacca 55440
agttcaagag cttcctgagc agaataaaca ttcgcctcag tattctttac agcttcttca 55500
gagagattac gagattcagc tacaaaacct gtaaaggttg cgtaggtatc attaattctc 55560
ttttgaagtc tttctttgct ttcttctgaa agtgcttgga atggtgaacc cataccttta 55620
aactcaccag ctttgatgac gttaattgtc acgccattct tttcaaatgc tttagttaat 55680
tcctgatgaa ccataatgac accaatagaa ccaacatctg catctggtga tgcaataatt 55740
tcttcagcag aagatgcaag agcgtatgca gcggaacaag cgaactcatc tacataagca 55800
ataattttct tttggcctct tgaagccata atgtgacgtg ctaattcaaa gcaacctgaa 55860
gcttcaccac caccagaatc gatgtgcaga acaatagtct taattgactc atctgctaaa 55920
gcttcgtcaa agcctctacg taaaccctca taagagctta atccacctgt acacattgca 55980
tcaatgaatg tcatacgatg ggttaaacca cccataatag ggataatggc aatgtcatct 56040
tttactttta aaagacttct tgcttcacct ttgggtttat caaagtttac tgctgcttgg 56100
acatcaccca gcaatctgtt attcacataa gttgctgctg agtgagctaa tgattcagtg 56160
gctagtaaag gttggttgaa taatctatca gcaagtctga agatattcga actcattttt 56220
actcacccta tttgtttaaa tctacagaga ctgaagagat aacataaata ccttcttcag 56280
caaagaattg aggtttgcta agagcaccag ttgccacgca tttatcgtta gcatcccaca 56340
agctatagtg tgagaccgtt gcagaagctg gaacagtaat gttaactgtg tcttctgagg 56400
caattaagcc attatccggt tcagaaaaat aaatagccac tggctgagta accttatttg 56460
ctgtagggtc tgccgttggg tctacattgt gtaaaataat agtcgttggg gttagcgtgg 56520
cgaggatttt gttcttacca tcaatagtta atgttcccat taattaaacc ttactttttg 56580
tttaaggact gttgaatggt taccagattc atctaccaca tttacaatca tatcatatac 56640
tttgcctttg acaagtactt tgtaatcatt ttgtgaaaaa atgtattcaa gtctgtttgt 56700
ttctttatta actgtcattg gagattgaaa tgcagtgtcg agggtaatga aagcggcttc 56760
aatgctatta acattgatac gtttattctc gcagttatat aactttacac ccaaaaggca 56820
tgaactgtca aatggaattt taacaatttc actacaattt cctgaaataa acggttttcc 56880
actcattggt gcatcaagta acctgcaaat agtgaaaacg tcagaggctc caccatcgct 56940
tacataacct gaaaggctga cccttgagcc agcctctaca gataacttgt cagtaatgat 57000
aagaacacct ctatacgaat gcacccgtgt agcatttgaa atagagataa cttcagccat 57060
tatttatttg ccttatttgc tgtgcttggg tcttttgctg aaggtgtctt tgcagtacct 57120
tctccagcag tcttatagcc atctcctgaa cggctttggc tatttggaga aagcttttca 57180
gatactggtt gagactcatc agcaggagga agaccaatat gctctctaag cttgttagat 57240
agctctttgt caacttccaa agcacctact gcaacagtct tctgaatata agaaccaatt 57300
gcttcaaggt ctggagtttc gatatcatca tatgtgattt gcacatgttc ttcatcatcc 57360
cacatattaa gagcgtaagt ctgtgcaact aaatcacggt taattacgtt cttaatttgc 57420
ttcagcagga tatctactga catcgctaat aagcttgtct tagaatctgc aagagagaat 57480
gaaccgtatt ttgactgacc catagcaaga acatctgaca taaatgccat cataatctgc 57540
ttggaatatc tgtcgataat agaacctgta tcgtatgctt tagcaccctg tctagaaact 57600
aatgagaact caaaaatatc ctctttagta tctgggtcga tatatctagg ccagattaaa 57660
cctgctctat cattagcaat catatcgtta acaacagttt tgcagtattg tacgaaagct 57720
ttcttttcag gttctgcatt ttcatccaga taatctggtg gtaaaccaat ctttggcata 57780
cctaccaagt ctcttgaaac accaacggct tcgtactctt caatctgaac tttatacttc 57840
cacggtacat aggcattaag taatggtgaa cgaccttctg ggtttccata ttcatcatca 57900
tacttaaaca gcatgaattt agctcgtggg agttttcttg ttagtggcct ttctccgaga 57960
ttaattgctc cagcaatatg tgaaacattt ctcagattct gcctaacacc agtaactttt 58020
ctaaagtctt cgtcaaaata ccacttatca agtgttgact ggtttctgat tggtaattta 58080
gcccatccaa ttagaccatc atcaaatttt gactggtact ttcctttctt accctgacgc 58140
ttcttataaa ctttctcgtt aacacagaac ccataagtgc agaatgacat tacagagtta 58200
ataaaatctg cccaatcatg ctccatgtca tccattaaag aattaaagaa gtctgctctt 58260
tcaagcattt tagggtcttg ctcttttccc tttggaggta caaatctcca gttgactttt 58320
ctgacaaaca tcttaataat atttacagat gctgctacag cagggtcacg catcattaat 58380
tggaaagttt taatactttc agggaacctt agtgcctgac gaggttcttc atagattctt 58440
ccattcttaa ccttcagacc caaagaacct acttcaccca ttctaaatgg tggtaagctt 58500
tcttgtgttt ctgtaatatc tgccattctt ctcacctagc tatcaacgtc taagcccctc 58560
atatgggttt cctctcacta agtctgtgtg agcacccatt gatggtggct tgaataattt 58620
aacttcgtta agactgttga aagcatcact ggtagcatcc acttggtcat ctttagtttt 58680
gccatcacca caaaagcctt caagctcttg gaaataagct tcgttccaac tacctctcaa 58740
aacttttaca agtccagctt cagaagcagc agaaaatccc gcaaagcggg taactttatc 58800
tttatttgtt ggtttagctc ttgcacgata gcctttctcg gcaagtttcc tgatgaggga 58860
tgtcgcgtag gatttaccag cagcgcctgg gtcttgaggg ataaaaatac cagttcgctt 58920
accgtcactt tcagcagtca aattaatttg tgtttcgact ccagagggtc tatctctaaa 58980
tcttactaca tcaatgatat aatagcaacc gtctttttta gatttaccca tcttaacacc 59040
tgctgtccag tctggattag ggttaatctc agatggtaaa gttgctgcta agtcccatgc 59100
tctaacatca aatacatctt ctgggagtga atcaacaatt tcacaccatt gtctttgcca 59160
atagtttgaa ccttctgcac gagccttcca gttaccgaaa cgaagtcttg caacgttcac 59220
aggagtgttg ttttcaagct taccgcgata ctttggttct aagaagtcaa ggattgggtt 59280
atcatcaatc gtaccagaga taaaggtgta tgtttgagga atctcaagag ggaacatttc 59340
aagaatcttg tctctctccc aatcagaaac catcacacca tcattcatta cataccaacg 59400
aatacgacca cacttttcag ggtctggata acctccttca tctaagaatg gctctaccca 59460
atcataaata aagtggtctc tgtctgggtt catggaaatc ttcatgtatg aatcaccttc 59520
agcaccagaa cgtagacggg tttgtaggta tgaaatctgt gaagcagaga agtgtgtacc 59580
ttcgtcaaag taaatagctg agtattcaat accctgatga ccttcagcgt gcttttcaag 59640
ttctagatag gtaaacttga tagtcgcccc agaagggaat gtgatagtca ttttctgctc 59700
gtgaggaatc ccaccaaact taccaaatag tttctttgca gcaggccata aaccaccttg 59760
taactgtgtt gtgtttcgac ggaaatacac agcattatag ttagggtctt caataaatct 59820
taaagagtcc attaacaatg cagcagtctt accagcacca gcagcaccac catagagaac 59880
caagtcagca ttagtgttta aaaagacctc ttgagaaccc ggctgagggg ctacatagtt 59940
cttatcagtc atcaatttaa agataaggcg aacttggtct ggtgtgtatc ttaataaagt 60000
cagaatttga gttggaagga atttagaggg gtctttaccg aatgatttga taatttcttt 60060
tacttcatca gaaagcccca actctccagc taggactttc ctaacatctt ccactcgctt 60120
ctgcttaaca gcatttaagt ccattaagcg cctccgcaaa tagaattatt cagagtctgt 60180
cgcagtctct tctttcttaa cttcgaactt tgcatcaaca gcatcaagaa gtgcatccat 60240
agatgcttct ttaatatcaa tgcctgttgc tacagataat ctgtctgcaa ttgccatgat 60300
agtattctgc ataaactcta acttcttgtt agcttcataa agttctttat aaacgttctc 60360
gctcattatg ttttctccaa ataataaatc ttgtttcccc ttaaaaaaga cggcacagag 60420
accgtcaagg agaaaccaca atgtacgtca gagacatact gtataagtct tcctataaag 60480
tataactctg taaaaaagcc ctgtctaaaa atactcttcg gtagcgaaag gaagaatact 60540
tatagcaggg cattattatt attttaattg agagagagta aaataacttg gagaatccga 60600
agggacttga acccttataa acctgttttg cagacaggca catatccatt tctgtcacgg 60660
attcagattg gaggaagata ccagacttga actggtacac cgatttctca gctactggca 60720
gtttagcaaa ctgctccctt accttttagg gttaatcttc cattatttct gtaatgcgtt 60780
cttcatttct ggtgttgcaa tagtgtcgat tacaccagtt ttgcgagcat catcaaacca 60840
gtctggtaag atacctgcta agaacccatt gatattcgtt ttaaggtact ctgcaataag 60900
ggcagcttct tcttcaatca tctgcacaac ttcatctgca taagctgtaa tctttgagat 60960
accttcatta atcttgctaa cagcttgatt agctaaatca gaaacagtat caagaccctt 61020
atcaatagcg tcttgtaagt cacttaacac accgttaaca ctgtctagtg tgctgttaat 61080
catgtcaata gctttttgac catactctgt agcaacaccc ataataccac tgaatggtgt 61140
acaaccaact tgttctcctg ctgcacccat aacattggaa tacccctttg ctacctgcat 61200
acgtgatgaa aactcatcaa tagacttttg accatagtct gttagggtct tagtagttgc 61260
tgtagtgctt gtcaggcttg ttgtaaagct gtttagaagg actgttgtaa gtccagcagc 61320
aacaagtttc tcattcattg tcgggtctgt tacagagcta atagagctta caagtgatgt 61380
agaggcagct acggttccac cgagaactgc tgcaccagta ataagtgggt tagaaaaccc 61440
tttaccggtt tttaagagat taaaaatctc cttaccttgt cctgtcatct ctttcattaa 61500
gcacctttga aattggtagt ccagtgggat ttgaaccctc ttctcatgtt tttcagacac 61560
gcgctttaac catataagct acttggacta taaattgggg tgacctacgg gatttgaacc 61620
cgtatagacc atgttcacag catgggtcat taccgtttat gataaggcca catttaagga 61680
ctctcgtaag aacccttaga agtggcagcg gcataaggat ttgaacctta ataggacagc 61740
ttcagagact gctgcattgc cagttatgct ataccgctaa aattggtact ccatatcgga 61800
ttcgaaccga tacataacac agattttaag tctggcctct ctgccaattg gagtaatgga 61860
gcattggcgg gggatgttgg aattgaacca acttcttcga tttcaaagac cgaggtttta 61920
accttgtaaa ctaatcccct ttaaatcttt actttctcag tggatgaata aagaatgcca 61980
acataactct ttcccttgag aatgctcttt cttctgggac aacacttttt agtttccatc 62040
caacataaat tctccagtaa aactgtttac caaagacttt aattgacgga ataaaagcga 62100
ataatcccca agcattgttg ttccacatta ggagataacc agtctgatta tcttcagggt 62160
cagaacttac gttgatatta cctctccact tagtaacatc ttttacatct cttcctaata 62220
catggtaaga gaagttataa gctttgtttc tccagagcca cccaactctc tgcatgtaga 62280
cacccagctt accaatcttt ctaatcttag cccatctttt aacgtgacct tcgtcaccat 62340
caatcgggtt gtcataggtc tccatccatc tgaatccaaa aggtagatgt cctttcttct 62400
cactgtagaa tggaactacg aaaggtgcta agataactgc taggattgct gcaattgggt 62460
ctagtaaagc taagaaaatc catgaagcat acttcaaata tctcattaaa tagcctcttt 62520
aaattggtgt tccaagacgg attcgaaccg tcactaatac aaggtttgag cttgcatcct 62580
ctgccaattg ggatactgga acatggtact cactaaagga cttgaacctt tttcttcatc 62640
ttgtaagggt gatgttttac catataaact aagcgagtgt gaaagaggtc tgaagttgtg 62700
ccgctaactc aactccgtgg aatttgttac ggtcttcagt tgacaccagc ggtctttcac 62760
ttgacctctg aattggtgct gctcacagga ctcgaacctg catttccatc cttacaaggg 62820
atgtgtaata gccaattata cgaaagcagc attgtttgga gcatccagag ggaatcgaac 62880
cctcaacctc agtttggaag actgtaattt tcccgtttaa actatggatg cactaattgg 62940
tggagtcact gagaattgaa ctcagttccc aagtttgcaa aactcgaatt ttaaccatat 63000
aaactatgac ccaaatgtct ggtacaggtg gagggaattg aacccatcgt cttactgatt 63060
aagagtcagc cgcataacca ttttgctaca cctgcattaa tttggtagga gacaagggat 63120
tcgaaccctc aaacaccaac ttctaaggat ggtaggttta ccaattaccg tcaatctccc 63180
attaaatctt tttagagaac ttctaagaac ctaagcaaca aggttccaca acaagctgta 63240
ataaccttag aagccctcta aaaagaccta aaaaggtctt tgcatagtct tgtttaatcc 63300
ggtgactaga acctttggag agcttgatat tatctcttct ccgtgacacc tactaggtgt 63360
tttatcagtg tgtacgacaa tacacgcggg ataccaaagc gacatccgaa tgaagcttgg 63420
ctttaaatct tattaggcgg taagtaaagg agtcgaaccc tcaccgtatc tctacagtgg 63480
caactgtttt caagacagtt tggctaccat tagccgctac ctacccctaa taagactttg 63540
gcatgggacg gaggagttga acccctttga aacggtgttg gaagccgttg ctcatgcctt 63600
agagtcttaa catcgtccca tactataatt cgaagcttac cgtattatct caacactgta 63660
aagccccttc tcagattatt tacatgttct ggaaaacatg agatacagac cacctcccta 63720
caggaagacc cgcttaggtc gtctaagtaa tctgctaact aattggtcga ggcggcagga 63780
ctcgaacccg catactccac ttactcggtt aacggcggtt tagaagaccg ctgagatacg 63840
ccccgtaatt atctttaaag actctcttag aaagccctta aagatgccca ccttattaat 63900
cataccgtgg gctagtacgc caaattcttt gatgagggat tggaagacct cactggtgtt 63960
tagcctatca agctactgcc agaaaaacat tgtcgtttgc atttatttta aatttgcaaa 64020
atagacgcta cgcaacgaaa actattcaga atatgtacaa caaatttccc cacattgtgt 64080
tacgtctaac gctcatgtat tggtgtttga attatacata ttctgaagta ttttagttac 64140
tataaagtag tcacatctca atgtcaacaa ctttattgaa attggttgta ggtgagggta 64200
tcgaaccctc ttctaacggt tatcagccgt ttgcattacc atttatgcta acccacaatt 64260
ggaggttcag atgggaatcg aacccacatt catagggctt atgagaccct tgcattacct 64320
tatctgcgac tgaaccatat tggtagaagt ggagggattc gaacccatcg cctgtcagat 64380
taaaagtctg ccgcatcacc attctgctac acttccatta aattatcttt gtagataagc 64440
tacccagact tgcttaaggt ctgtagcgat gtgccttttt gggtttaaat ctagaattta 64500
ccctttcacc ctttgtggtc gcatactcac aggtaagctt aataacttat ctacaaagat 64560
aattaccaga ccgttgttta tcatcttaag tgctaccatt acaccaactc gacacctgcc 64620
gagtgaagga atcgaacctt ctccttttct ttaccgagaa atagattgtt taagttttgc 64680
tgtaaacggt cttctgtaaa tttgttgagc cagaccaagt tttaaatttt tcaaaattaa 64740
attggcttga aattttaagt aacttgctgt atttggtctt ctgctccttt aaagaatact 64800
cgtaagaact ctttaaggga ggggctaaat agccctctca aaattggcgg ttacgaaggg 64860
atttgaaccc tcatcatctc ccgtgacagg ggagtatttt aaccaaatta aactacataa 64920
cctttgttgg tgtgccgtgt aggagtcgaa cctaccgagt ctcaatgaca agggatttac 64980
agtccccacc gctaccatct acgggataac gacacattta atttctattc aggctaagga 65040
cttgcacctt acaaatcggt gagcaatcgc ttctctccta aatttggcgg ttagtcaggg 65100
attcgaaccc tgtgccattc gcttaacagg cgaccgcaca taccttatgt gcttcctaac 65160
catgtttagt attaccagac cacatctttc aatttttccg ctaaaaattt aataaatatt 65220
gctgtaaacg gtcttctgta aatttggagg cgggtgcagg agtcgaacct gccgatacca 65280
tgctaatgag acatgtgaga cgccctttct ctatacccgc aattcttaat gaccagacca 65340
aatctcttct ttgtccgatt taccttcaga tgaataagat taagtttgct gtaattggtc 65400
ttctgtcaaa actggcgttc cagaagggat ttgaaccctc aaatatccac tttgaaagag 65460
tggtgactat accattttgt ctactggaac attaatttgg tctctgttgg aggacttgaa 65520
cctccggcct taccgcccca aacggaacgc tctaccaagc tgagctaaac agagataaac 65580
ttttcaaact ctatgtaacc actttaacat tattttttag tgattgtcaa gaactttttt 65640
aaaatatttt tcagtatctt agaaaagctc tcgtttcgtt tctatgtaga acatattaaa 65700
gggtatcaaa cacattgtca ataccctttt taaaactttt taccagatat aacggtcaat 65760
cattactgct ttgagcatta cgctgatagg gtcaaacttc tcaccaccaa gcagagcttt 65820
taaagtggct ggagagaaac cagataccat tgctacaccg ttatccttaa cagatacttc 65880
gcaagtacca ttacggtttg ctaagtacca gaataccagt tgtggcattt cgtatccagc 65940
tttcttgtac ttactctgga ttgcttcaaa gtttgtgcga ccgttagctc catcaacctg 66000
attgaactcc atatctgaga agatgataag cttacttggc atatctttct gagtcaagtt 66060
atttctttta cctgtctcaa gaatacggtc aaaagctgct tgtaagttag ttgagccata 66120
ttcaacgtgc cgcatcacct gacgatgacg gtttcgtaaa tcaccactca gttcgatgaa 66180
gtgagggttt gttgagtaaa ccattaactc atttttaaag caacctgtat tgcgttctgc 66240
tacatacaat gcaagtgata caccaatatc cagagcagta atggagccaa aattcaccca 66300
agacattgaa cgtgaaacat cagacataca gagaatgttt tcaccctctg ccatccagtt 66360
tggtagtgct ttccactgct cattagcaac atctgcatta ccatacttaa cagacttaat 66420
cacatcgtat gggtaaacag caccagcatt aatcttagtc tcaccctttg ataaggactc 66480
gatgtaagct ttgtagcgtt ctccatcttt gcggttaaac agtttttggt aacgtgcagc 66540
agcaagtgaa ggaatcttac tgtagtcaat cttgccaaac tcattagcag agattttttg 66600
ctcaactgta tcagagagcg cagacaacag tgtgcgatac tctttctcac tcaagttggc 66660
aaacttacag aaacgtttta caaactgctt atgacgtggt ttgactcgtg gtagccactt 66720
ggctgccaaa cctgctgttg cagggtctag taatgctgct tctaaatgtt tgaaggcatc 66780
tgtctcgaaa cgagtaccta cgaagatttt gaagtcatca aaacgaccaa gttctgcaat 66840
cttatccata atgcgaagaa cctgtgtagg ctctaaaact ttatcttcaa ttgcttgaag 66900
caaaacagtt ctgaaggctt tacgctcacc aataccttct cgtacatctc gcatatgcag 66960
taaaatacga actgcaacat caacatcctc acgcaaagct ttgtagaaca ggtctggtaa 67020
gatttctacg ttgctacggc ttgaaccagc agctttgtaa aagtctacaa gagcagacat 67080
tgatgaagta tggtttacag caccattttc agttcgacct gcatgaaggt gcgcatgttt 67140
aaataattcg ctcatatttt actctcttct ctcatttgtt gtttgatgtg acagacttta 67200
gagtaacttt gtagagcctg tcaacatctt ttaaaattat tttttgagag ttgcaacagc 67260
agagaacgtt ggtttgtttt ctgtctgaga ctgactactg ttatcaggag tcttcttagt 67320
atctgctgca attgctttca tctcacctgc tgagtgggtc atgatagttt taccgacctc 67380
aatcatgctc ttgatggtta cgtcgtctgt gtttaacccg aactctgcaa gcttagcagc 67440
gtcccttgta acaatcgctt caaacaactt tgcagcaaac tcagcagaat tatcaatggt 67500
cagttgagct tttacgagtg agcttttgtt acgagagcct tttggtcttc cagatgggtt 67560
tccagactgg ccttttttaa actggccttt gtttgttctg tttttcattg gtatgcctct 67620
tataagacct cttaaaaggc ttttaagata aaagatagat atccagaaag atatctattt 67680
aaatagcctt tttagaggaa ccttttaagt tatcttctaa gtatttataa gcctatacct 67740
tgtcaagtgc tttgtcaaca acttttttaa cttgcaatag ttcttgactt gttgtatgga 67800
ttactgtacc atcttactta aagctgtagg tctgccttgt tctacaaagg agacttaatg 67860
agaaaatcaa ataaaccgaa gaaaggtaag aataccaatc actgtaagga atcaaaaagg 67920
gtagaattaa tctactattc gtcctctgaa attggtctgt acctgttctt tcaaaattac 67980
agaagacaag aggattatct atgtgtagtt cccaattaga aatcgcagat attatagatt 68040
tatatcaagc tgcaaagagt catggctata taacctcaat tggaaagaat agtcactatg 68100
atgctttgac tggaatgtat ttcagggcaa tggctcaatc tagtgaacaa cacttagtgg 68160
tttcttccag tgagtttact tcgtttctct attgcagcaa aattataaat cgcaggagaa 68220
ctgaaaaatg ttaacagtaa gttttaatta caatagtgac ggctctgtat caattaactc 68280
accatatgca aatgacctac ttaaagagct agtcaatcag tgtgagaggg gtcttcacta 68340
tgttcaaaat tcttttaaac agaagactat tgctaataac ctgatgcgtg taacagtgac 68400
ggcatcaaat ccaaactatg actttgatag tgaaagtccg tactcattag ttgcgcttgg 68460
agagagcagc caattcaaac ttgtttgcca cgactcagaa acattcctta aggtattttc 68520
caaccttatc cataataata agtacagcta cgttgatggt agtgtaaact tctatccagc 68580
aaactataca tgtttgttaa ttgataatat gagaaaaagt aagcaagagc ctacagaaat 68640
ttcatttgat gtgaactcta gtccagacgc agaaacaagt aagaactttg atatgagtta 68700
cgcattgtca ctcagtaaga aatccgagtt cattgattat gtcaatggat ttggttttaa 68760
gtttgatgag agcatgaatc ttaaaaaact taagaaccta cttaagacca aagcttaagt 68820
gtgaataggg gctgatgccc ctttaactat ttataaggat atttaatgaa agctaagagt 68880
gcaaaagact tttattgctt cctacaatcc tatatcaatt ccgtgcaaaa tggtgaaagg 68940
tacaacctta atgatgttat cccaacacct ttgacatgga gactcagtaa atggcctgaa 69000
gaagatatta caccgacaag cgaacaacgc acctacaaac ctgaaatcaa tattccagat 69060
tctgagaatc tattatatcc aatgttccat attgttggtc ttggaagatt ccttatggat 69120
atccagtatg taattggtaa aggctataag gttaaaggta ttgttgttgg tgatgtatct 69180
ccgcatcaca aaggttattt tagactaaac gcacatttag aggctaaaaa gaaatgatta 69240
aagcaaagac ttacccagac ttcaaagagt ttgtgaaggt cttcattgca aacgtaaaag 69300
ctggtaagag atatgatttt agaacatatc aagaggctat tttaccgctt acctatagtt 69360
cgtattggcc tgaagctgat attgcagaag ttgaggagtt tgactacaaa ccagactaca 69420
aagtcccatt tagtgatgag ttgctttaca gcatcggtgc tcaaatgaga acttctgact 69480
tcttcatgga cttacaatat gcaattatca atggtaaaga cgttgataca atttattgtg 69540
aatggctggc aagggttaag ccgttctcaa tgttgaatgc taagttgaag gatgctatca 69600
agccaccagc aatcactcag cagccaacca atcaaacagt gaatgaaggt ggcacactta 69660
ccctaagcgt tattgcaacc aacgccactg gatatcaatg gaagaagggt ggtgaggata 69720
tcactagtgc cacatctgca acctacacaa aacagtctgt agcaccttct gatgctggtt 69780
cttatacttg tgttgtgtct ggagaggctg ggacaagtgt tacctcagat gcggcaacag 69840
ttactgtcaa cgcactgcct gtgattacac agcaaccttc tagccagacc atcaatgaag 69900
gtgaaaacat caacttggaa gtgactgcaa caggtgcaac aggttatcag tggaagaaag 69960
gtgaggaaga catcctcgat gcaactactg caacttacac caaagaaggt gcaactgctg 70020
ctgatgcagg aagctataca tgtgtagtta ctggtgcagg tggctctgta acatctaatg 70080
cagcaacagt tactgttaac ccagcagggg aggcataatg caactctcaa gaaaaggttt 70140
agaagctatt aagttctttg aaggtcttaa gctagaggct tacgaagatt ctgctggaat 70200
cccaacaatt gggtacggaa caatccgtat ttctggcaaa cctgttaaga tgggtatgaa 70260
gattaccgct gaacaagctg aacagtatct tctcgcagat gttgaaaagt tcgttgcagc 70320
agtgaacaaa gtcatcaagg ttccaacttc tcagaatgag tttgacgcac ttgtaagtga 70380
aacatacaac attggcatca cggctatgca ggactctaca tttatcaagc gccacaacgc 70440
tggtgataag gtcggttgtg cagaagctat gcagtggtgg aacaaggtta cagtcaaagg 70500
taagaaggtt acctcaaacg gcctgaaaaa cagacgtaga atggaagctg acatttatct 70560
tgacagtgta tatccaaagt aatatcttca taggctcctt cgggagcctt ttttattttc 70620
taaggagaaa actatgaaac tttgggctag tgactttggg acttttaaat acactcgtaa 70680
tggctcgctt gtacgcattg ttggaaacaa tgtagtttca agaggtgaca aggtgtatac 70740
acggtttact gtagagcttg ttgaactgtc acctattgag tctgtgaaca atggtctgtt 70800
caagtttgag acttacaatg tcaatgagca tggacaattc aaccctcttg gcgaaagtgg 70860
acttgatatt atttcagagc acccattgac aaaagagcaa cttgcaggtt actataaatc 70920
tgttctggaa aggcagttag caacacatga acaagaagct aactaccatt tgcagcattg 70980
cgaaacttta agagcaaaaa tcgaacaagc ggagagaggt ttttatgaat aaagatgtta 71040
gagatatcca agtaaaagtt gacacgtttg gccatgatgg tcgagaagac ataggttttg 71100
ccgatgcagt aatatacaag tgcaacggag atttattcct tcagtttaca gggtctggaa 71160
gcacactcca tcgagtacga gatgatgaag atggttgccc tgtatttggt tggtttgata 71220
tagagttccc tctttatgca atccactatc cagatggtgg agaagactgg actactcaat 71280
caatctttga tgagcttaac ggtgttccag ttgagcaaga agagcaagag cctgttgaac 71340
tgacctttac cttcatcaaa gaagagaaag taggtgactt gtcagtaacc gaagcaattc 71400
aagtctcaca ggttattcgt taatgagtaa agtacaggtt gttttcccta tttgtgactt 71460
ctcactagag cgtgaacttg acctgtacga agaaattact gacgaaatta tctggtctgt 71520
tgtagaagag gctatcaaga aactgtatgg tggcctctta aacccatcaa gtaaaaagct 71580
caatactaaa caagtagctg atacacacac ttcctatgat gcctacaaca aaccttttga 71640
gaatacctgc tttgacctta tggtaggcaa taacaaagtc aactatttct tcgtcagaga 71700
gtttaacgat gagtaagctc catgtcacag tgtacaaaaa cttctcagat attaaagagt 71760
ctttaacaaa taagcttgac ttacaacgta aaaggctctt tttaatgtat gacatagaca 71820
actacaacca tcctaaagag tttaactaca aagatggtac gaaggtcgtt gaatttgaag 71880
attctgtgac ggtgtatgtc aaatatgact taccagcaaa gtacataggg atgttagagt 71940
attacatatt caaacataca ggtatgcgtg gtgaagctgt caagatatct tctatagaag 72000
tttttgagaa acctaacaca caacttaaaa aatatttaat gaggaaactg taatgtccga 72060
agagcaacag gatatcattc cacaagtaac acttgtacaa cactttggaa atattgaagg 72120
gtgtgttgca ctttttcaac caagcatcaa ctctcctgca aaagtttgca agctgactat 72180
gaatgttaac aacatcagtg tttgccttgt tgatgaagtt caatatttca agtttaacga 72240
cagagaggtt gatgctgcac tattgaagta ccgagcaaac ctcgaaaaag atattgacca 72300
caaagaactt gtaacactgt ttggtgacct tcacaagctt cttgaaaagg ttatgaagcg 72360
tacatactac atgaacaatg gttcaatcat caccactttg atttcaccat gtatttcaga 72420
gccgatttta actgatgatg gtggatacta cgtggtagca tcagcagact ctgattggtg 72480
gatgaagaac acagcactta aaacagtgat tgaggctatc cgtgaacata tcccttcatt 72540
cagcccgtgg aaaggtaaaa gtgatgattt tatcgcacta ttgagtgaag agagtaacaa 72600
gcgcagtgca ttactgccta aaaaatactc ttgaccgagt atacaaaact gattatgatg 72660
ggagctatcg agaggtagtt ccctttttag tttctagata agagacatgg tgaaagaatg 72720
aaaaaactaa ctacagttga agactactac aaactttccc tgttagaaca gtatcgcaga 72780
agccagaata tcagaaaatg ctatggtaag cacgctgaag gtgattttta ccattgctat 72840
gatgcagatt taaaaggcgt aacacctaga gggaaagttc tgcaaagatt tgttgacctt 72900
gaatggaaca aacgattgag aggggttgga aaatgattta cgaagaaaga tacaagatag 72960
attttcagga tactcgccat cacacttctt taagagtgac gaaaccaaat ggagataccg 73020
gtatcatagc acattttggt ggtgattact ggtatggcac aggttgcttt gaaggctaca 73080
ctcaagaata cctgaaagct ttctacagag actttgcaaa tgactacaac agagttgttg 73140
acgaaaagaa taagtgcatt aagcatgaac accatgccag aggctgccta agtattgtta 73200
tgggactggc attcttctta gcaacgttac tggcagtatc agtaatcagc tacacagctc 73260
aagatttgac aattacacag attaccgcaa aggtacaaga tatttggtac ttgtatgctg 73320
ttcctttagc tggtatcatc atctcactaa tgaggttcag agttcataag aaacgactta 73380
aggactctga ggttaaactt gaagaggtaa gtaaagaatg caacctacaa ttatagctgt 73440
atgtgttcgt tttgcaatag ctgaaatgat taacaaggca atcttaaaag atgcctatgg 73500
agagactaag taatgattaa gacacctgta ccaatttttg gattcccttc tatagaagag 73560
tttaaagtct atcttgacaa aaacttctac aacgagcagc ctgttacttt gctgaagagt 73620
gacttatcag agcttcttga tatggttatc aaggcaactt ctgagaagga acctgagcag 73680
aaagctgaga agaagactag taagaaatcc gataagaaga ctgaaaagtc tgagtagtaa 73740
cttgaggggt tacttgatag cccctttgta gaaacttaga aggtagcgaa tatgaaaatt 73800
aaaaaagtcg ttcaaaaagc aatgcttgac aactcaacta aagataaaat gtacaaagag 73860
atttgtgata agttgaattg ttcaagacat gctgctaagg ctcttgtgca ttgctttatc 73920
ttggaatgct cagaagctta tatgaaacat atagcttttg aaagttctaa cttactaggt 73980
gatgtagaag ttggtgagaa gctaaaagaa cctgagatga aaacagttcc taaagttggt 74040
aatacatacc ctcttaaaga tttcaagact ggaaagattg ttgcaaaagg tgtagtagaa 74100
tctgtctatc atgatggtaa atacttactt aaaatatttg agtatgatag ccgctataca 74160
cacttgtgtg gtcttacatt tttagtaaca gaagaagacc tcattaagaa caatgacaac 74220
aagtttgcag taccagatta ccaagtttta cgataatggt gtgatagcta tggaaaaaga 74280
taacttaaaa gaagttgaag gtaactacct agtccttgac tggaatgatg ttcgagaagc 74340
attatccaaa gaaagccttg acatactaga gcagcttgtc ttctcagtac ggcatatcag 74400
agaagttaag aatggtaaag agccacttga aggaatcttt gtagagaagt cttatccgtt 74460
ctatgaagac actttacaga aagttaaaat gtactttaaa cagaagaacc gtaaagtggt 74520
cactatggta tctcttggtg gacagagtat gtctgtgatg gaagatatca accctaagag 74580
accaagtaaa ggtgtctgta taaaaatcac aggtcatgaa gagtatatct cagtggatga 74640
ttttagtgtg ttctgtaatg gttctgctgt ctttaatggg tttcactatg atatccgagt 74700
gtacccaatc aagggagatg atatcatgat gcagatattt gataacaaca atggattcac 74760
tcatttttat cagacaacca agtcatcatt aaaaacagtt ctggaaacac tgatttaaaa 74820
tatcttaaga gcctccttaa ttgggggctt tttattttta taaaatttta tcggttgtcc 74880
caacctctgg gtcaccatca tatgtgaaca aaatatctat ccttcttaac aaccttctta 74940
atcttactct ctatatagtt atatagtact atatagtatc ttagtaacta tctagtggtc 75000
ttaatagccc tctgtataac ccttctaact agccctctaa caagccttct aaacactctt 75060
ctaacaagcc ttcttaacaa cctatacaat caccttaacc acctctctat accctcttag 75120
aagctcctat acacctctta acccattatg ttagctatga taccctctat aggtgttcac 75180
taggtattac taagcttcta tatagactgt tttaacaaac ttaacaggga tatctttaca 75240
gaccttatct cacaaggtct aacacggtac taacaaggta tcttataagc ctgtttctac 75300
ctgtattttt aaaagactgt ataggtaatt attcagaatt ttaaaatttt tataaaatag 75360
gactatcaag tcatttttta gtgtctttga aggaactgaa aaatatctga ttaggatgta 75420
tatctgcttg gtagccctta acagcccctt aacaccccct ttgcagaatt ttaaaatatc 75480
cttaacaatc tcttaacagt tctcacatct tcttaacaac tctttaaagg tcttaacagt 75540
ttcttaacag aactaacaag gctgtaacag ttttctaaca agtgctgaat aggctttaca 75600
gatgcttaac agatgactaa caagttaatt ttaaagagtt attttgttaa gataattaac 75660
aagttcctaa caagtgttta atctttttaa caagctatta acatgacaac tcttctttct 75720
tatcgttccc tttcctttct tagtgattcc ttaaacatac ctcttaaaag ctctctatag 75780
ctctttatag gctcttttaa gtctctccct agtatttacc cctcaactgt atggatagct 75840
ctttataggg ctttacagga gcttttaagg ctatgccaaa gactcttaca aggcttttac 75900
gtggtcttaa cagagattta acaaggggct aacaaggggc taacaagtgc cttaaaagca 75960
taactatgta gggtttgtta agaaattgtt aagaaattgt taagggggct agcatttacg 76020
taacttttaa gcaactttta cgtaacttta tagccacttt taagctttcc atagcatacc 76080
ttttaagtat tatcaagccc tttttagtta ttcacaagat agctaacaag atgataaagc 76140
ttacaccctt ataagattct tttaaggctc ttttaagcct ctataaagct ttataaaggg 76200
cttttaatgg tgagttaatg caatccctta agtcaggtta ataaggctgt tatagagctt 76260
tatagagcgt ttaaatggtt gcccttccct ctcgggttat gggcttatat ttaagttgat 76320
ttaataaaag gctatagagt aagatataga gttattgctt tttaaggtat gagatagatt 76380
ctatcgaatc aatgggtaag attctggaaa gggaaaggga tttgataaga cgtaaaaaag 76440
cccctatgaa aaggagcttt gaaggtatct ggggctattt tagccccgtt gattgacaat 76500
ttacagattt ttgattgtct cgtcgatatc tttatcaata tcttttaaag ttttttctaa 76560
attcttgata ttgaaagata agccttcacc gatggtttta gagtaacctt caggccgctg 76620
gttatgcagt tcgatttctt ccacattgga taagtttacc atctcaccat ttgcagcata 76680
ggccattgaa acagtgttta caggggcttt ataaaacttg tttaaggact tgatagaacg 76740
ctcataagcc attctataac tgtttaaaag gtttttaacg tgtttgtgaa cgtttttaca 76800
ctcatctaag taaagcccta aaaacatcgg gagaagttcc gcgacagctt gcataaagtg 76860
agagactaca acggttgcaa cattacgacg gtgaacgtag taagccccat tttctgactt 76920
ggtgatggtt gcaaagtgta caccattaca gcgaaattcg taagtgtctt taacaggttt 76980
tgacattaca gcacttgaac gaatgtaatt gatagctttg aacttgttca tctttgaatc 77040
tcctagttta aggactttac ttagtgggga aggttatccc ctcccgttga ttagtaattt 77100
acttgttttg acttaccttt gcaagcaatt ttttaagctt atctataaag aatcttttaa 77160
gtgaatccct tttagataaa ccccctcgaa caaagttacc aatgtcaaga ggggttgtca 77220
acactttttt aaaagttttt tgtaagttat tgattaactg atgaatattt tttcaggttt 77280
tacatagttc atcttttggc ctttgagcaa atcatcccag attaaagcgg cgatatgaga 77340
ataaccggat tcacgtaata gcttcattat tttacttctc gtttcgaatc tggttgcgta 77400
gtattcacta ttcaaggccg ttttattccc ttccttgtca gtgtgaacga tacgcatcac 77460
atgaaagggg cttgtaccct cgcctttgtt ccattgttca aagtatttta ggcactgcat 77520
aggtgtcatc ttatcactta cttgagttga ccaagatgcg ccagctttgt cggtaattac 77580
ggttacataa gccatttttg aatctcctag tttaaggact ttacttagtg gggaggttat 77640
ccctcccgtt gattgacaat ttacagactt ttatcaggct tgtctagtct ttttcaatga 77700
taatctcata ttggccgtca acactatcaa aataaacgcc gtccttgtct tctgtcttaa 77760
agtaacattg tatatcatca ctggcggggt agtcttgtct tgctatctga caaacctgta 77820
tagcattttc tttacttcct gtaaactcct caagcccttt aaaattgcat gaatacccat 77880
tacaaataga aaggataaga taaacggtta taagcatgat ttaagccccc taaaagcccc 77940
ttataggggc taaatagtgt ttataaggtg attgtataga ttattagaaa aagctcttta 78000
aatctctttc tgtaatgttt cctgtcagtg tgtccatgat gtcacaaaaa acctgaatac 78060
tgtctgattg ttggatactt ttagcgagaa gttctagagg ctcctcaata tagtttaaat 78120
tcgggttatc cagtgaagga agacaaaaag gcgaaaacat agcattaaat tcagtgatgt 78180
aaactttttt aatccagttt atatcaaaat actctgtaaa acttgccacg ctagacatat 78240
tatcgcaaga gaaatcatcg tttaactcat tttctggaat atcaagttct aagataacaa 78300
tgttttgagt tttcatttga aaggccgctg atatagtggc atttcctagc gcttgttgaa 78360
ttccttccga ttttatctct tccgcatcgt cgccatagat tttattttct ggataaacat 78420
aaaaattgcc gtccatatcc gaacaattcc atgcacctga tggcttatca ccgttattaa 78480
tcaggttgat aaagttttct tgagtagtac cgtgaaagca tttcattttt gaatctccta 78540
gtttaaggat ttagcggtaa agctctttat ctggggctta ctttatcaaa ctataagccc 78600
ccttgtaaag tactttaacg attatttttg acaactttta aaactgcgcg ataacctaag 78660
caataagaat caccgaagag agaccggccc atgaaagagt cctctttgat atcctttgaa 78720
gtaacagttt tttctacgcc gtgaaaaagg actatatcgc caactttgat atctttgatg 78780
tgagtagttt caatgtttaa gccgttaatg atagccattc tagaatctcc tagtttaagg 78840
actttactta gtggggaagg tcattccctc cccgttgatt agtaatttac ttgttttaaa 78900
aaccaaaatc aagaggaatt ttttaaagtt ctgaataaat tacgtcgcag atatcacgaa 78960
aatcgtgatt tgttaaatta cgttgtaacc atgttgcgaa aaagtcagcg attgcaaaat 79020
ttgcgccata ctcgacggat agccatactt taacagcaaa acgaaacatt ttcaaatcca 79080
gctctctatt cattacctta gatttgccta acatgtcagc catttcatat tgtgcgccgt 79140
ctagcgtgat acccgctgca acgtcaatca gtgagttttt tacgctttca acgtctaaga 79200
taacactttg aatcttcccg ataatgtttt gcagtgtttc gtctggcgct gatgacgtga 79260
taacattaat ccggttgacg gtcaaacgtg cttttaagat taagaattca cggttattaa 79320
tagccatttt taagttctcc aatttaggat ttagcggtaa agctctttat ctgggggctt 79380
actttatcga actataagcc ccttgtaaag tactttacac taaatgcagc tttttgcctt 79440
ttaccatgat gtaacttgat gcgctattgc tgcacacatc ttggtaaatt ctgcgcaaac 79500
ggcctttgta gtatactttc cagctggttg gtatcttttt cccatatccg gtagcagttt 79560
tttgaagtcc agcaagttgc caatccaaaa tatgttgttc cgcttgtaaa tcctcaaagt 79620
tcatcacgcc gtctacatag tggcgtagag tgacatttag agtacggcca ttgttagtag 79680
tatacatttt tgaatctcct agtttaagga ctttacttag tggggaaggt cattccctcc 79740
ccgttgatgt gtaatttaat atatctaaaa atcagtgtca actgttttgt tttcgtttgc 79800
tcaactcctg aacaattaca gcgtaatagt cttttaagtc actgatattt aaagcattta 79860
tcagggcgtt ataatcatcg acaagctgaa aatcctcttt gaaaatgtcg ctgatttgat 79920
aagtttttga ttgaaatctg aagctcattt ctaaccgttt aagttgttca tcacttaaac 79980
gctccagaaa gctcactaca aggttattat taacctgttg agtaattgcc tcacttgcca 80040
gattgtaggc gctgtaagcc ttgtaaagtg ctataatggc gatgattgca gtgataccca 80100
tcattaacat gatgttccct cttaatttaa tttttaaaga tattcatcac tgaatctttt 80160
aaggcttcta agcgttctaa tacttcagat tctgcgtctg cgttgttcac ttcctctttt 80220
agcctgtcat ctaaatcatc gccgtccaag taagaccaat caaacacata tccaatacta 80280
aaactatcta aaaccgtgtt accgtttgca tctacaactt ttgcagcaag gtagtattct 80340
gaagcctcta aatccctctt taaagatttt tgcattgatg cataagcttc taatgatgga 80400
ttttcacggc cttgttttgc atagtcacta gcaaggcaac ttaaactata gtttaaagga 80460
atagcatatt tgtatccatt gcgttcatag ttaggattct taacagttac cccgccatga 80520
tgtttatcag taaattccca gctatcttcc attacataag atggttgata agtgttaaca 80580
gcctcataaa tgatggaaaa tttttctttt acaacttcaa aggtagcgtc tacagttttc 80640
atttttgaat ctcctagttt aaggacttga tttagtggta aagctcttta tctggggctt 80700
aagatatcag cttttaagcc ccttgtaaag tactttatca gattaaattg ccagttaatg 80760
ataagtgcat tttatagcct tttttggtaa aaacgttcac tttaccgagt gattttacac 80820
ctttaaaaag gttgtttgaa ctgcgtttta aggttagttg cttatcagct ttcacgcccc 80880
accttacagc agtttgtttt ccgttgtcaa ccacttttcc gcataacttt ttagccataa 80940
cgcgagcgtc atcacgagtc tttgcagtaa ttacaacgtt tttgataata gccattttta 81000
agttctccaa tttaggattt agtggtaaag ctctttatct ggggcttact ttatcgaact 81060
ataagcccct tgtaaagtac tttattagtt aattttgaaa attgtcacat aaccatcact 81120
cccgatgatt tgtgataccc cagctttttc tacttgatag cccatatttt caagatggta 81180
aacagcatca tcgtaaccat atgcgccacc ctgataacgt cgcttatcgc attttatcag 81240
gtttttaccg cgtccagcaa gtgcattcag tacggctttt tcacttgaca gagtaccgtc 81300
taacatggtt gccgtgtaat ggttgattct attaccattg acatcatatt taaaattgaa 81360
tgcgtgaaca acaatcaatt tgccgttaaa tttttcatca agtgacttct ccagtgcgtt 81420
gcggaagttg tctttattga tgaatttcgg tgcacgataa gccattttag aatctcctag 81480
tttaaggtaa gttagtaaag ctctttatct ggggcttaag atatcagctt ttaagcccct 81540
tgtaaagtac tttttatata tcgtaatagt aaaccgctgt ctgttttata tgtcgctttg 81600
tattctttgt taggtagcaa ttagagttaa ttgccgtttc tacatagttg caataatcag 81660
cttctgatat tctacgccat acgccgcaca cttgcatata aaaacgtttt gcctttgtgt 81720
taacgttggt tgtaaccatc aattcgcgac tgtacatcat tttggattct ccagtttagg 81780
aagtgggctt gcttgcccgt ctgatgcaaa tattagataa ccatactcaa agagtcaata 81840
ggaattttaa aaatattttt gcaagaattg taatgaaacg ggtgcgcgcg actaccacaa 81900
aacagaacta caatcaagaa ttatttttca atccgctaca aatttttctg ttgactttta 81960
ttttcagatg tggtagcctt gtgagaaatg gcgacatccc cacctacacc cacctacctg 82020
cacaattcta aaatgatttt tcttgcccga cctgagcggg gtctgcacgt tttgaaaata 82080
aaaaagctct ttggagatta cctgcacagt ttgaaaatga attttctctt tggagttcaa 82140
aattctctct acagattttt gtccagaact ctacctgcac aatcctaaaa tgaaaaagcc 82200
ctccttacct gaaaaattta ccagataaga aaggcttagt ctattgtgaa tattctacaa 82260
agtttcttag ctctttgcgt ctaagaaggt aacttcctgt gaagagaaaa tttccttaac 82320
atcctcatca gttcttgcat agatatatgt atctgcatca ctgccaaatg ttactgcaat 82380
cctgtgattg ctcttcgaca ggtgggtaac tagagcatta cttagagaca tcttttcaac 82440
cggagggtta ctttgatgta tcttaccttt tcttcgtctg aagagggtaa ctgtatcatc 82500
tgtgaacact actacacccg caaagtattc tggttcacag tagttcttcc aacctgagtg 82560
taacttgtgg tacatctctc cttcatagac atacagatac acatctacac tcttcatcac 82620
ctgattgatg ataactgtga cagcttgagg gtcaagtcca ccgatacctg caccaatcat 82680
aggtaagcca actttcttca actgattaac ttcacagtat ctgttaagtt gcttcaaaga 82740
tgactctaaa gcactgtatc gggcatcttt accagtgtga agctgagtgt aaaggttagc 82800
tatacggcct tgttttaaac gtgctacaga gaagttgcct agtaaatttt cacagggttt 82860
gtggcctata ccacctgcat acagataaac ttctgtatcg gtctcatatg cttttgggta 82920
aagtttagaa atcttgttag cgatacccgc acccattaag ttcatacagt tgcaaccgtg 82980
accaatgata tcaaacttac ctttatcaaa tgcggagaag atatccccat tgatgatttt 83040
tacaataccc atttagcatc tctcccaaga ttctttatct tctgaaggtt ttacacgttg 83100
tccttcgtgg tctacccaga tacatccaca accttcacag attactggca ttgcataacc 83160
agctttaaag tcgtcttcag tgataagacc ttttaagtca cctgtatcac gtccaaacat 83220
ttcgatagaa cagtctttgc agaagtcagc cattgataat tacctcttga atgatgatac 83280
ctttaccaca acgactttta aagtcttcca atggctggga gataaagcca cgtaagcaat 83340
aactgaatgc taattttttc ctgtagacta tcacatcatt tgctgtaaaa ggaatcactt 83400
catttcttcc aataaatact gctgaccgga taattttccg tgtcttttta tctctggcta 83460
gaattacttt cataatttac cttctcagtc agttcataca agtcaacatg ctcgatgctg 83520
tctatgttga tatcaatctt tttgaaacgt tccatcactt cattgtcagt catccatatc 83580
agacaccaat gattctggat ggattcttca agaagctcaa cacaatcttt aaaagttttt 83640
gtatcacttc ttgttccgac tactgcgatt gtcccataca accacttacc attaccataa 83700
gatacttgtg ctacaccgac ttctttatct ttctcgtagt aaatggctac aaagtctctt 83760
ccattaatct gcatagttac tgcctcgttt taagaactgc taagatttct ttagagtctg 83820
gtaagaacca tgtgtagtga ttctctttga tttctggatg agtgtcaacc tttattttgc 83880
aatctttacc aacatgttta agttgtgctg tgacaccttg cataaagata tgacacatca 83940
cgttatccat aatggttggt gagaaatcaa cccgcatata agtttctttt ggtcgtggta 84000
gcagtaagta cccttctaca tagaaagtat cttcattctc tgtcattgct gtttatttcc 84060
taacaaaatc atatcaataa ggttattagc ttttattgta cactggtaaa aaccatcacg 84120
ataaaactca agggctgctg ccacatcact gcctgtgtta cctttgatga tataccgtga 84180
ttcttcttga gtgtacttgt cactctcact tacttggaag tttttgaatg tactgttcac 84240
tttgttcgtt gaacaccctg acataattat cagtaacaca aacattataa aactctggct 84300
tagttttttc atatgttagt acctcttttg tatgcttatt cttagcaagc acatctttca 84360
aatcgttttt gtaattggcg cttagtgtgg ctaaaccttc ctgataagca tctttagcaa 84420
cgcttattag tttctcattg tttagctcag cttcagcaac ttgatagtct ctgtaagaat 84480
atcctcccca gacacctgct ccaactaaga aaacaattat gaaagtagct tgacagaact 84540
ctttaaatgt catttaacaa tctcctgaaa tgaaaaaggc tcccgaagga gcctgtatct 84600
taaccttaac cgaggacttt agcaaggaca tttgcagcca tcgaagaagc ttttgcagca 84660
gcagctaccc cagctttgat agcgttatcc ttgatagcag ataccgttgc agcatcagta 84720
aagagataag tagttgtttt agaaccactt gtaaaggaaa gcataagagc agtttccaga 84780
ttgaaagagc gatacggagc accttcttta acgatacctt cagcacctaa ctctgaagcc 84840
atacccatat catagatagt catcaggttt tctttgtgtg cagtcgttga accagcacct 84900
ttgacgtgtt tcttaacatt cagaagagca cggtattctc ggatgttacc atctgctttt 84960
acattgacag cacggaagat tttaccctca aagttgcttt tgataatgtt acgaactact 85020
tcagatttgt ttgccgtaac gtccagtgct accgtaatga tattgttcat aagttttctc 85080
tcaatgtttt actaacatag ttttaatgtg cggaaggatt ctgtttacct tctgaacatt 85140
cttctctgta tcttctataa aacatactat attaaagtaa ggaagaatgc aagactgaat 85200
catcctaact ttcaaacttt ctgcatttat agagttagtt ccaaaaccac gcataaaaag 85260
catgtaatcg tgctctatgt tatgccttaa aaacatctca gtaggtattc tctgagattc 85320
tcctctcgct gtcaagagtc caatggcagc gtgactagca attgcatcaa taatactaaa 85380
aacatacgtt ggttttgctc ttacagagtc aagaagatta gtgtactgtg agaatgaccc 85440
atcagtcaat tccgttgagc aatcttcgtg attaaaattt gtaagaacac catcaatatc 85500
tgctaagatg aggttacctt tttctacaag gtctttattg actatgacta aactgtcacg 85560
tcttacacct atagattgcc cagtctcttt gtccacaacg ttcacaattg gttctgatgt 85620
gtggaactct tcaagtgaac atacaacttc tcttgcagtg tcttcaaaat ccctgcactg 85680
ttctggagtc catatacagg aatcaagacc taactcatga ggatgcaagt cgtaagcatg 85740
acacagtact tcatcagact ttcttaattt tttcatgatt taccttagaa atgtttgttg 85800
caaattcaca ttgagcgatg actttcatgt tgttatagac atcttgagag tatttcttag 85860
ctttaggaat tttgtacgaa tacccagcat tatatgatgc taaaactttt tgtaaagtct 85920
tctttgactt tggttgacca tgtaccttcg tccaaaactc aagctctttg tgtgtttcct 85980
ttgcagcata gtcaaaatct tttaagagtt tctttttagc cacattagga ctgattttgt 86040
tacgctttac aacagtcttc aagtgattct ggaagatacc atagtcatgg gtctttttat 86100
tctccatatt cagacctaac tctgactctt gtaaggctat agctgctaga gtgatacccc 86160
aacctttacc catgttgttc tcaccatatt ggtaagcttt taacatgttt actttttgac 86220
taattgatag ctctgggcag tcaactgcat aagataagtg tgctgtaaac attaagcaca 86280
aacctagaat caacttcttc attggttctc ctgtttagtt tatcgtgaca acaattatac 86340
agcactctgc acaaaataca aataaaaaag gccgccgaag cagcctttta agaaattact 86400
tattagattt cacgtttacg ttgggagatg agttcaccag cagtgccaac gatgacatgc 86460
aaagcttcag tcttaccatc ccatgctttc agcactttac gttgattatc aacgattgct 86520
gcaacttcat aacgagagga acgcatcttc atatcgttgt aatcagttgg aacagataca 86580
acatctcgtg gatggacacg aaccttcagg atggtatcac ctgagaagca acgaacataa 86640
tcccaagcac caacatgaag accttgagag cgagttacgt tacggttgtt atcaaccatc 86700
caacgtggca tctctacaat gttacccaaa tcattaggta ctttgtaggt gtgagagtca 86760
accagcttgc cttcacgagt agagactttc ttccaaccaa tgatgtagcc ttcttcatca 86820
atttcaacat caaggtgaga tacaaacccc caaagttgtt ctacagagtc tttagatggg 86880
ttttccatca gtttttcgaa gaacattaca agtcgttcaa aacctttatc accagtcttc 86940
atcatgtgaa gaatacggtc aaccagagta gaacgcatct caacagcacc gtagaatagc 87000
ttatcacctt tgatggtgat agcaccctga gtgaaatttt cgatagactt acgaatgttc 87060
atcagttcga aagctttctt atactcaccg gatacaacag ccatcacgat ttctttgtag 87120
ttcggatggg tagattcaac gatttcagaa tctgcaccga aagtcatgat aacagagtcg 87180
ccagtaatca tgtactcaat ctggttgcca ttctgcatag cttcatgcag tttatttacc 87240
ggggttttct gtacatcctc tttaacagcc tttttaactg gcttgtcagc tttcttttta 87300
actgacttag caacttgctt cagagtcttc gtagctggtt tagaagctac ctttttctta 87360
ccttttaagg ttgcttcatg acgctctaca gcacgaccaa ctgaacgggt tgaagtattg 87420
aacttctgtg cgatagctgt tttagtcagc ttaccttctt taaccagttc gtaaatttct 87480
gcgtcaatct gtgctttagt tttagtagtc atcttattac tctctcttgt tagttaataa 87540
atcattttgt gaggttattc taaggggctt tgaagcccct tgtcaaacac t 87591

Claims (10)

1.一株高裂解性沙门氏菌噬菌体RDP-SA-19009，其特征在于，透过电镜观察，该噬菌体头部为廿面体，且立体对称，头部长54.754nm，尾部长度为178.143nm，为有尾噬菌体目长尾噬菌体科，基因全长87591bp，不具有毒力基因和溶原基因，保藏编号CGMCC No.21993。
2.如权利要求1所述的沙门氏菌噬菌体RDP-SA-19009，其特征在于，该噬菌体在80℃高温条件下作用30min，效价保持在1.0×106pfu/mL以上，具有较高的生物活性，对温度有较高的耐受性。
3.如权利要求1所述的沙门氏菌噬菌体RDP-SA-19009，其特征在于，该噬菌体RDP-SA-19009可高效感染宿主菌，其最佳感染复数为0.001。
4.如权利要求1所述的沙门氏菌噬菌体RDP-SA-19009，其特征在于，该噬菌体RDP-SA-19009在体外抑制其宿主菌BS-19009长达12h。
5.如权利要求1所述的沙门氏菌噬菌体RDP-SA-19009在制备预防和或治疗沙门氏菌引起的疾病的药物中的用途。
6.如权利要求1所述的沙门氏菌噬菌体RDP-SA-19009在制备预防和或治疗沙门氏菌引起的疾病的饲料中的用途。
7.一种用于防治沙门氏菌的杀菌组合物，其特征在于，包括有效量的如权利要求1所述的沙门氏菌噬菌体RDP-SA-19009。
8.如权利要求7所述的用于防治沙门氏菌的杀菌组合物，其特征在于，还包括用于抗沙门氏菌的抗生素。
9.一种预防和或治疗沙门氏菌引起的疾病的添加剂，其有效成分包括权利要求1所述的沙门氏菌噬菌体RDP-SA-19009。
10.一种清洁剂或消毒剂，其特征在于，包括如权利要求1所述的沙门氏菌噬菌体RDP-SA-19009。
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Cited By (5)

* Cited by examiner, † Cited by third party
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CN116987673A (zh) * 2023-05-15 2023-11-03 佛山科学技术学院 沙门氏菌噬菌体及其组合物及应用

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Publication number Priority date Publication date Assignee Title
CN114231499A (zh) * 2021-11-18 2022-03-25 青岛嘉智生物技术有限公司 噬菌体及其应用
CN114231499B (zh) * 2021-11-18 2024-05-17 青岛嘉智生物技术有限公司 噬菌体及其应用
CN114921423A (zh) * 2022-06-28 2022-08-19 华南农业大学 一株多重耐药肯塔基沙门菌烈性噬菌体及其制备方法和应用
CN114921423B (zh) * 2022-06-28 2023-11-28 华南农业大学 一株多重耐药肯塔基沙门菌烈性噬菌体及其制备方法和应用
CN115747172A (zh) * 2022-10-24 2023-03-07 华中农业大学 一株耐热沙门氏菌烈性噬菌体毒株及其应用
CN115747172B (zh) * 2022-10-24 2024-06-11 华中农业大学 一株耐热沙门氏菌烈性噬菌体毒株及其应用
CN115948348A (zh) * 2022-11-09 2023-04-11 聊城大学 一种宽谱禽源沙门菌噬菌体及其应用和组合物
CN115948348B (zh) * 2022-11-09 2024-01-30 聊城大学 一种宽谱禽源沙门菌噬菌体及其应用和组合物
CN116987673A (zh) * 2023-05-15 2023-11-03 佛山科学技术学院 沙门氏菌噬菌体及其组合物及应用

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