CN113396924A - Bactericidal composition for preventing and treating macadamia nut anthracnose - Google Patents
Bactericidal composition for preventing and treating macadamia nut anthracnose Download PDFInfo
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- CN113396924A CN113396924A CN202110684271.3A CN202110684271A CN113396924A CN 113396924 A CN113396924 A CN 113396924A CN 202110684271 A CN202110684271 A CN 202110684271A CN 113396924 A CN113396924 A CN 113396924A
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- anthracnose
- bergenin
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
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- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
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Abstract
The invention belongs to the technical field of pesticides, and particularly relates to a sterilization composition for preventing and treating macadimia nut anthracnose. The effective component of the bactericidal composition for preventing and treating macadimia nut anthracnose is formed by binary compounding of bergapten and pyraclostrobin, fluopyram or bromothalonil. The bactericidal composition for preventing and treating macadimia nut anthracnose is prepared by compounding two effective components, can delay the generation of drug resistance of the macadimia nut anthracnose to a compounded medicament, reduces the risk caused by independently applying bergenin, and can play a certain role in protecting the bergenin.
Description
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a sterilization composition for preventing and treating macadimia nut anthracnose.
Background
Macadamia nut, also known as macadamia nut, is a nut plant originally produced in macadamia, not only has higher economic value, but also has good nutritional value and medicinal value, and is vegetatively reputed as the king of dried fruits. The macadimia nuts have more diseases and insect pests, more than 30 diseases and more than 300 insect pests, and the more serious diseases include damping off, root rot, flower blight, anthracnose, ulceration and gray mold. Wherein the anthracnose mainly damages leaves, young shoots and fruits of the macadimia nuts, the young shoots of the macadimia nuts are dead and the young leaves are withered and yellow, black plaques appear on the surfaces of the macadimia nuts, young fruits and shells are black brown, and the young fruits and the shells gradually develop into black conidiospores, so that the yield and the quality of the macadimia nuts are influenced. Chemical agents are common means for controlling anthracnose, but long-term application of a single chemical agent can cause the anthracnose to generate drug resistance, the current anthracnose generates different degrees of drug resistance to the existing chemical agents, fruit growers generally increase the application dosage of the chemical agents in order to improve the control effect, so that the generation of the drug resistance is accelerated, and meanwhile, the problem of pesticide residue is also caused.
The inhibition effect of 18 coumarin compounds on the citrus anthracnose is determined by adopting a growth rate method, and the isocoumarin compound bergenin has the strongest inhibition activity on the citrus anthracnose, and EC is determined50It was 7.2766. mu.g/mL. Therefore, the anthracnose can be effectively prevented by utilizing bergenin. Like chemical agents, if the active ingredient is singly applied for a long time, the diseases are easy to generate drug resistance.
The compounding of different pesticide effective components is an effective shortcut for developing new pesticide varieties. After different pesticide active ingredients are compounded, three action types can be shown: additive, synergistic and antagonistic. Through a large number of experiments, the inventor finds that when bergenin is binary compounded with pyraclostrobin, fluopyram or bromothalonil, the remarkable synergistic effect can be shown in a certain mass ratio range, and the composition has important significance for protecting bergenin medicaments and delaying the drug resistance of diseases.
The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person skilled in the art.
Disclosure of Invention
The invention aims to provide a bactericidal composition for preventing and treating macadimia nut anthracnose, which overcomes the defect that the disease is easy to generate drug resistance by singly applying bergenin for a long time.
In order to achieve the purpose, the invention provides a bactericidal composition for preventing and treating macadimia nut anthracnose, which is prepared by binary compounding of bergapten and pyraclostrobin, fluopyram or bromothalonil as effective components.
Preferably, the mass ratio of bergapten to pyraclostrobin is 1-10: 20-1.
Preferably, the mass ratio of bergapten to fluopyram is 1-30: 15-1.
Preferably, the mass ratio of bergapten to bromothalonil is 1-40: 40-1.
Compared with the prior art, the invention has the following beneficial effects:
(1) the bactericidal composition for controlling macadimia nut anthracnose has obvious synergistic effect when binary compounding is carried out, and compared with a single medicament, the bactericidal composition improves the control effect on the macadimia nut anthracnose, and can reduce the application dosage and times of pesticides, reduce the control cost and reduce the pesticide residue of macadimia nuts.
(2) The bactericidal composition for preventing and treating macadimia nut anthracnose is prepared by compounding two effective components, can delay the generation of drug resistance of the macadimia nut anthracnose to a compounded medicament, reduces the risk caused by independently applying bergenin, and can play a certain role in protecting the bergenin.
Detailed Description
The following detailed description of specific embodiments of the invention is provided, but it should be understood that the scope of the invention is not limited to the specific embodiments.
Examples: indoor bioactivity test of bergenin compound
Reagent to be tested: 98.8% bergenin technical, 98% pyraclostrobin technical, 96% fluopyram technical and 95% bromothalonil technical
Test germs: the pathogenic bacteria of the macadimia nut anthracnose are collected from the pathogenic parts of the macadimia nut anthracnose and separated and purified in a laboratory
The test method comprises the following steps: refer to indoor bioassay test criteria for pesticides NY/T1156.2-2006 section 2: petri dish method for pathogenic fungus hypha growth inhibition test
1. Dissolving the raw medicine with ethanol, diluting with 0.1% Tween-80, respectively preparing single-dose mother liquor, setting multiple groups of ratios, and setting multiple gradient mass concentrations for use according to equal ratio method for each single dose and each group of ratio mixture;
2. quantitatively adding the pre-melted PDA culture medium into a sterile conical flask under aseptic conditions, sequentially and quantitatively sucking liquid medicine from low concentration to high concentration, respectively adding into the conical flasks, and sufficiently shaking. Then, the mixture was poured into 4 dishes 9cm in diameter in equal amounts to prepare drug-containing plates, and blank controls containing only ethanol and 0.1% Tween-80 were added to the plates, and 3 treatments were repeated for each treatment.
3. Under the aseptic condition, punching holes on the edges of bacterial colonies of the macadimia nut anthracnose pathogenic bacteria by using a sterilization puncher with the diameter of 5mm to prepare a bacterial cake with the diameter of 5 mm; inoculating the fungus cake in the center of the medicated plate with the hypha facing upwards, covering with a dish, and culturing at 25 deg.C in an incubator.
4. When the diameter of the blank control colony is more than 2/3 of the diameter of the culture dish, measuring the diameter of the colony by a caliper, vertically measuring the diameter of each colony by a cross method once, taking the average value, calculating the hypha growth inhibition rate of each treatment, carrying out regression analysis on the numerical value of the medicament concentration and the hypha growth inhibition rate value by DPS software, calculating the EC50 of each treatment medicament, and calculating the cotoxicity coefficient (CTC value) of the mixture according to the Sun Tapei method.
Hypha growth inhibition (%) [ (blank control colony diameter-5) - (drug-treated colony diameter-5) ]/[ (blank control colony diameter-5) ] × 100;
measured virulence index (ATI) ═ (standard agent EC 50/test agent EC50) × 100;
theoretical virulence index (TTI) ═ a agent virulence index × percentage of a in the mixture + B agent virulence index × percentage of B in the mixture;
co-toxicity coefficient (CTC) × 100 [ measured toxicity index (ATI) of the mixture)/Theoretical Toxicity Index (TTI) of the mixture ].
Criteria are divided according to joint action:
the co-toxicity coefficient (CTC) is more than or equal to 120 and shows a synergistic effect;
the co-toxicity coefficient (CTC) is less than or equal to 80, and the antagonism is shown;
80< co-toxicity coefficient (CTC) <120 showed additive effect.
The results are shown in tables 1-3.
TABLE 1 determination of the indoor biological Activity of bergapten and pyraclostrobin on the pathogen of macadamia anthracnose
| Name and proportion of the medicament | EC50(mg/L) | ATI | TTI | CTC |
| Bergenin | 9.84 | 100 | -- | -- |
| Pyraclostrobin (Kresoxim-methyl) | 4.43 | 222.12 | -- | -- |
| Bergenin 1: pyraclostrobin 20 | 3.43 | 286.88 | 216.31 | 132.63 |
| Bergenin 1: pyraclostrobin 16 | 3.17 | 310.41 | 214.94 | 144.42 |
| Bergenin 1: pyraclostrobin 12 | 2.94 | 334.69 | 212.73 | 157.33 |
| Bergenin 1: pyraclostrobin 8 | 2.27 | 433.48 | 205.88 | 207.85 |
| Bergenin 1: pyraclostrobin 4 | 1.93 | 509.84 | 197.70 | 257.89 |
| Bergenin 1: pyraclostrobin 1 | 1.66 | 592.77 | 161.06 | 368.04 |
| Bergenin 2: pyraclostrobin 1 | 4.83 | 203.73 | 140.71 | 144.79 |
| Bergenin 4: pyraclostrobin 1 | 5.92 | 166.22 | 124.42 | 133.59 |
| Bergenin 6: pyraclostrobin 1 | 3.17 | 310.41 | 117.45 | 264.30 |
| Bergenin 8: pyraclostrobin 1 | 2.88 | 341.67 | 113.57 | 300.84 |
| Bergenin 10: pyraclostrobin 1 | 4.17 | 235.97 | 111.10 | 212.39 |
As can be seen from Table 1, the bergenin and the pyraclostrobin are compounded at a mass ratio of 1-10: the co-toxicity coefficient to the pathogenic bacteria of the macadimia nut anthracnose within the range of 20-1 is more than 120, and the synergistic effect is obvious.
TABLE 2 determination of the biological Activity of bergenin and Fluopyram in combination against the pathogen of macadamia anthracnose
| Name and proportion of the medicament | EC50(mg/L) | ATI | TTI | CTC |
| Bergenin | 9.84 | 100.00 | -- | -- |
| Fluopyram | 3.29 | 299.09 | -- | -- |
| Bergenin 1: fluopyram 15 | 1.93 | 509.84 | 286.65 | 177.87 |
| Bergenin 1: fluopyram 12 | 1.85 | 531.89 | 283.77 | 187.44 |
| Bergenin 1: fluopyram 9 | 1.66 | 592.77 | 279.18 | 212.33 |
| Bergenin 1: fluopyram 7 | 1.53 | 643.14 | 274.20 | 234.55 |
| Bergenin 1: fluopyram 5 | 1.35 | 728.89 | 265.91 | 274.11 |
| Bergenin 1: fluopyram 3 | 1.19 | 826.89 | 249.32 | 331.66 |
| Bergenin 1: fluopyram 1 | 2.83 | 347.70 | 199.54 | 174.25 |
| Bergenin 5: fluopyram 1 | 3.91 | 251.66 | 133.18 | 188.96 |
| Bergenin 10: fluopyram 1 | 4.22 | 233.18 | 118.10 | 197.44 |
| Bergenin 15: fluopyram 1 | 5.51 | 178.58 | 112.44 | 158.82 |
| Bergenin 20: fluopyram 1 | 4.88 | 201.64 | 109.48 | 184.18 |
| Bergenin 25: fluopyram 1 | 3.46 | 284.39 | 107.66 | 264.17 |
| Bergenin 30: fluopyram 1 | 4.42 | 222.62 | 106.42 | 209.19 |
As can be seen from Table 2, the bergenin and fluopyram are compounded in a mass ratio of 1-30: 15-1, the co-toxicity coefficient to the pathogenic bacteria of the macadimia nut anthracnose is more than 120, and the synergistic effect is obvious.
TABLE 3 indoor bioactivity assay of bergenin and bromothalonil in combination against macadamia nut anthracnose pathogens
As can be seen from Table 3, the bergenin and bromothalonil are compounded in the weight ratio of 1-40: the co-toxicity coefficient to the pathogenic bacteria of the macadimia nut anthracnose within the range of 40-1 is more than 120, and the synergistic effect is obvious.
As can be seen from tables 1-3, the bergapten, pyraclostrobin, fluopyram or bromothalonil compound of the invention has a synergistic effect on macadamia nut anthracnose within a certain mass ratio range.
The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (4)
1. The bactericidal composition for preventing and treating macadimia nut anthracnose is characterized in that the effective components of the bactericidal composition are formed by binary compounding of bergapten and pyraclostrobin, fluopyram or bromothalonil.
2. The bactericidal composition for controlling macadimia nut anthracnose according to claim 1, wherein the mass ratio of bergapten to pyraclostrobin is 1-10: 20-1.
3. The bactericidal composition for controlling macadimia nut anthracnose according to claim 1, wherein the mass ratio of bergenin to fluopyram is 1-30: 15-1.
4. The bactericidal composition for controlling macadimia nut anthracnose according to claim 1, wherein the mass ratio of bergapten to bromothalonil is 1-40: 40-1.
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| CN202110684271.3A CN113396924B (en) | 2021-06-21 | 2021-06-21 | Bactericidal composition for preventing and treating macadamia nut anthracnose |
| CN202210339862.1A CN114794130B (en) | 2021-06-21 | 2021-06-21 | Agent for preventing and treating anthracnose of macadimia nut |
| CN202210339858.5A CN114794129B (en) | 2021-06-21 | 2021-06-21 | Pesticide composition for preventing and treating anthracnose of macadimia nut |
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| CN202210339862.1A Division CN114794130B (en) | 2021-06-21 | 2021-06-21 | Agent for preventing and treating anthracnose of macadimia nut |
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| CN115868396A (en) * | 2022-12-16 | 2023-03-31 | 广西南亚热带农业科学研究所 | Ecological seedling raising substrate for macadamia nuts |
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| CN114794130B (en) | 2023-09-26 |
| CN114794130A (en) | 2022-07-29 |
| CN113396924B (en) | 2022-03-29 |
| CN114794129A (en) | 2022-07-29 |
| CN114794129B (en) | 2023-09-26 |
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