Bactericidal composition for preventing and treating pineapple anthracnose
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a bactericidal composition for preventing and treating pineapple anthracnose.
Background
Pineapple is one of tropical fruits, has more than 70 varieties, and is one of four famous fruits in Lingnan. Anthracnose is a common fungal disease in the pineapple planting process, which occurs almost all year round. After the pineapple suffers from anthracnose, round or nearly round chlorosis small spots are firstly generated on the leaf surface of the pineapple, then the small spots gradually expand to the periphery to form large spots with different sizes from oval to long circle, the disease spots are connected into pieces in the later period, the production of the pineapple is seriously affected, but the anthracnose has different degrees of drug resistance to currently used drugs, so that the prevention and treatment effect of the current drugs on the pineapple anthracnose is poor.
The coumoxystrobin is a methoxy acrylate protective bactericide developed by Shenyang chemical research institute in China, has a wide bactericidal spectrum, and can effectively prevent and treat various diseases such as downy mildew, late blight, scab, anthracnose, leaf mold and the like. The action mechanism is to block the electron transfer between cytochrome b and cytochrome c, thereby inhibiting the respiration of mitochondria, causing the ATP synthesis to be blocked, causing the fungi to lack energy supply and gradually lose the infection ability, but the action site is single and is easy to generate resistance.
The SDHI bactericide, namely a succinate dehydrogenase inhibitor bactericide, affects the respiration of pathogenic fungi by inhibiting succinate dehydrogenase in tricarboxylic acid cycle during mitochondrial respiration of pathogenic bacteria, hinders energy metabolism, inhibits the growth of pathogenic bacteria, and causes the death of pathogenic bacteria. The coumoxystrobin and the fluxapyroxad are different in action site from coumoxystrobin, the generation of drug resistance of an agent can be delayed by compounding different pesticides, the compound pesticide has an ideal use effect, and no related report about the compounding of coumoxystrobin and sedaxane, fluopyram or isopyrazam exists at present.
The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person skilled in the art.
Disclosure of Invention
The invention aims to provide a bactericidal composition for preventing and treating pineapple anthracnose, so as to overcome the defect that the effect of the current medicament on preventing and treating the pineapple anthracnose is poor due to different degrees of drug resistance of the anthracnose to the currently used medicament.
In order to achieve the purpose, the invention provides a bactericidal composition for preventing and treating pineapple anthracnose, which is prepared by compounding effective components of coumoxystrobin and SDHI bactericides, wherein the SDHI bactericides are any one of sedaxane, fluopyram and isopyrazam.
Preferably, the mass ratio of coumoxystrobin to sedaxane is 1-7: 30-1.
Preferably, the mass ratio of coumoxystrobin to fluopyram is 1-5: 50-1.
Preferably, the mass ratio of coumoxystrobin to isopyrazam is 1-10: 20-1.
The invention also provides a pesticide preparation comprising the bactericidal composition for preventing and treating the pineapple anthracnose, wherein the mass of the effective component of the bactericidal composition accounts for 0.8-65% of the total mass of the pesticide preparation, and the balance is an auxiliary agent allowable in the pesticide pharmacy.
Preferably, the pesticide preparation is in the form of wettable powder, water dispersible granules, a suspending agent, a microcapsule suspending agent, an emulsion in water, an ultra-low volume liquid or an aqueous solution; the auxiliary agents allowed in the agricultural pharmacy comprise a plurality of known substances such as a dispersing agent, a wetting agent, a disintegrating agent, a binder, a thickening agent, a defoaming agent, an antifreeze agent, a solvent, an emulsifier, a high-molecular capsule wall material, a pH regulator and the like, and have no special requirement, and specific components and dosage can be selected according to the formula requirement.
Preferably, the mass of the active ingredient of the bactericidal composition accounts for 32% of the total mass of the pesticide preparation.
Compared with the prior art, the invention has the following beneficial effects:
after the pesticide composition is compounded, the active ingredients have no mutual resistance, and the pesticide composition has a good synergistic effect, can improve the prevention and treatment effect of pineapple anthracnose, reduce the pesticide application dose, reduce the pesticide residue and relieve the environmental pressure; in addition, as the sterilization action sites of the coumoxystrobin and the SDHI bactericide are different, the generation of drug resistance of pathogenic bacteria can be effectively delayed, and the prevention and treatment cost of pineapple anthracnose is reduced.
Detailed Description
The following detailed description of specific embodiments of the invention is provided, but it should be understood that the scope of the invention is not limited to the specific embodiments.
Examples: indoor biological activity test after compounding coumoxystrobin and SDHI bactericide
Reagent to be tested: 96% coumoxystrobin technical, 95% sedaxane technical, 96% fluopyram technical and 92% isopyrazam technical
Test germs: pineapple anthracnose bacteria collected from field
Test work: culturing to 3-4 pineapple seedlings at true leaf stage
The test method comprises the following steps:
1. dissolving the raw materials in ethanol, diluting with 0.1% Tween-80, respectively preparing single mother liquor, setting multiple groups of ratios, and setting 5-6 gradient mass concentrations of each single agent and each group of mixture according to equal ratio method;
2. culturing the tested bacteria in culture medium, and sterilizing after producing sporeWashing spore with water to obtain a solution with a concentration of 1 × 105Taking 10 mu L of prepared spore suspension liquid by a micro-sampler, inoculating the spore suspension liquid on the leaves of the pineapple seedlings, wherein each plant has 30 inoculation points, uniformly spraying liquid medicine on the leaves until the leaves are completely wet after inoculating for 24 hours, transferring the pineapple seedlings into a moisture preserving box with the relative humidity of 95 percent after the liquid medicine is naturally dried in air, culturing for 24 hours in the dark, and then culturing under the conditions of 25 ℃, illumination intensity of 20000Lux and humidity of 85 percent;
each treatment was 3, 4 replicates and a blank treatment containing ethanol alone and 0.1% tween-80 was set.
3. When the incidence rate of the blank control reaches more than 50 percent, measuring the diameter of the disease spot once by a vernier caliper by a cross-over vertical method, taking an average value, calculating the control effect of each treatment, carrying out regression analysis on the logarithm value of the medicament concentration and the control effect probability value by DPS software, calculating the EC50 of each treatment medicament, and calculating the co-toxicity coefficient (CTC value) of the mixed medicament according to the Sun Yupei method.
The control effect is [ (blank control lesion diameter-medicament treatment lesion diameter)/blank control lesion diameter ] × 100;
measured virulence index (ATI) ═ (standard agent EC 50/test agent EC50) × 100;
theoretical virulence index (TTI) ═ a agent virulence index × percentage of a in the mixture + B agent virulence index × percentage of B in the mixture;
co-toxicity coefficient (CTC) × 100 [ measured toxicity index (ATI) of the mixture)/Theoretical Toxicity Index (TTI) of the mixture ].
Criteria are divided according to joint action: the co-toxicity coefficient (CTC) is more than or equal to 120 and shows a synergistic effect; the co-toxicity coefficient (CTC) is less than or equal to 80, and the antagonism is shown; 80< co-toxicity coefficient (CTC) <120 showed additive effect. The results are shown in tables 1-3.
TABLE 1 indoor determination of biological Activity of Scutellaria Terminalia ester and Epoxiconazole Amylum Fluconazole on pineapple anthracnose pathogen
Name and proportion of the medicament
|
EC50(mg/L)
|
ATI
|
TTI
|
CTC
|
Eugenia caryophyllata ester
|
4.12
|
--
|
100
|
--
|
Sedaxane
|
0.46
|
--
|
896.65
|
--
|
Coumoxystrobin 1: sedaxane 30
|
0.28
|
1471.43
|
869.99
|
169.13
|
Coumoxystrobin 1: sedaxane 25
|
0.14
|
2942.86
|
865.06
|
340.19
|
Coumoxystrobin 1: sedaxane 20
|
0.21
|
1961.90
|
857.76
|
228.72
|
Coumoxystrobin 1: sedaxane 15
|
0.33
|
1248.48
|
845.92
|
147.59
|
Coumoxystrobin 1: sedaxane 10
|
0.16
|
2575.00
|
823.32
|
312.76
|
Coumoxystrobin 1: sedaxane 5
|
0.22
|
1872.73
|
763.04
|
245.43
|
Coumoxystrobin 1: sedaxane 1
|
0.49
|
840.82
|
497.83
|
168.90
|
Coumoxystrobin 2: sedaxane 1
|
0.67
|
614.93
|
365.22
|
168.37
|
Coumoxystrobin 3: sedaxane 1
|
0.51
|
807.84
|
298.91
|
270.26
|
Coumoxystrobin 4: sedaxane 1
|
0.39
|
1056.41
|
259.13
|
407.68
|
Coumoxystrobin 5: sedaxane 1
|
0.43
|
958.14
|
232.61
|
411.91
|
Coumoxystrobin 6: sedaxane 1
|
0.64
|
643.75
|
213.66
|
301.29
|
Coumoxystrobin 7: sedaxane 1
|
0.44
|
535.06
|
199.46
|
268.26 |
As can be seen from Table 1, after the coumoxystrobin and the sedaxane are compounded, the weight ratio of the coumoxystrobin to the sedaxane is 1-7: the co-toxicity coefficient to the pineapple anthracnose pathogen is more than 120 within the range of 30-1, and the synergistic effect is shown.
TABLE 2 determination of indoor biological Activity of coumoxystrobin and Fluopyram against pineapple anthracnose pathogen
Name and proportion of the medicament
|
EC50(mg/L)
|
ATI
|
TTI
|
CTC
|
Eugenia caryophyllata ester
|
4.12
|
--
|
100
|
--
|
Sedaxane
|
3.23
|
--
|
127.55
|
--
|
Coumoxystrobin 1: fluopyram 50
|
2.16
|
190.74
|
127.01
|
150.17
|
Coumoxystrobin 1: fluopyram 40
|
1.71
|
240.94
|
126.88
|
189.89
|
Coumoxystrobin 1: fluopyram 30
|
0.94
|
438.30
|
126.67
|
346.03
|
Coumoxystrobin 1: fluopyram 20
|
0.77
|
535.06
|
126.24
|
423.84
|
Coumoxystrobin 1: fluopyram 10
|
1.25
|
329.60
|
125.05
|
263.58
|
Coumoxystrobin 1: fluopyram 1
|
1.91
|
215.71
|
113.78
|
189.59
|
Coumoxystrobin 2: fluopyram 1
|
2.37
|
173.84
|
109.18
|
159.22
|
Coumoxystrobin 3: fluopyram 1
|
1.14
|
361.40
|
106.89
|
338.11
|
Coumoxystrobin 4: fluopyram 1
|
2.72
|
151.47
|
105.51
|
143.56
|
Coumoxystrobin 5: fluopyram 1
|
3.06
|
134.64
|
104.59
|
128.73 |
As can be seen from the table 2, after the coumoxystrobin and the fluopyram which are effective components of the invention are compounded, the weight ratio of the coumoxystrobin to the fluopyram is 1-5: the co-toxicity coefficient to the pineapple anthracnose pathogen is more than 120 within the range of 50-1, and the synergistic effect is shown.
TABLE 3 indoor determination of biological Activity of Scutellaria amethystoides and Pyrazolaphthalendronide on pineapple anthracnose pathogen
As can be seen from Table 3, after the coumoxystrobin and the isopyrazam which are effective components of the invention are compounded, the mass ratio of the coumoxystrobin to the isopyrazam is 1-5: the co-toxicity coefficient to the pineapple anthracnose pathogen is more than 120 within the range of 50-1, and the synergistic effect is shown.
In conclusion, the bactericidal composition of coumoxystrobin and sedaxane, fluopyram or isopyrazam has a good synergistic effect in a certain mass ratio range, and can improve the prevention and treatment effect of pineapple anthracnose.
The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.