CN105831165A - Microbial pesticide for preventing and treating apple pest and disease damages and preparation method of microbial pesticide - Google Patents
Microbial pesticide for preventing and treating apple pest and disease damages and preparation method of microbial pesticide Download PDFInfo
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- CN105831165A CN105831165A CN201610276079.XA CN201610276079A CN105831165A CN 105831165 A CN105831165 A CN 105831165A CN 201610276079 A CN201610276079 A CN 201610276079A CN 105831165 A CN105831165 A CN 105831165A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/54—1,3-Diazines; Hydrogenated 1,3-diazines
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N53/00—Biocides, pest repellants or attractants, or plant growth regulators containing cyclopropane carboxylic acids or derivatives thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- General Health & Medical Sciences (AREA)
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Abstract
The invention provides a microbial pesticide for preventing and treating apple pest and disease damages .The microbial pesticide is prepared from, by weight, 16-24 parts of fenpropathrin, 11-19 parts of emulgator, 19-27 parts of organic solvent, 1-3 parts of an antifreezing agent, 3-7 parts of a thickening agent, 21-29 parts of water, 2-6 parts of purple bergenia herb extract, 2-4 parts of azoxystrobin, 1-3 parts of plantain herb water extract and 1-3 parts of chlorophyll .The invention further provides a preparation method of the microbial pesticide for preventing and treating the apple pest and disease damages .In this way, the prepared microbial pesticide has a good effect on preventing and treating of the common apple pest and disease damages, is good in effect of restraining and resisting apple bacteria, has no side effects on apple plants or apples in the process of carrying out preventing and treating on the apple plants and is high in safety, the yield of the apples is obviously increased, and the contents of carbohydrate, pectin and fructose in the apples are remarkably increased.
Description
Technical field
The invention belongs to preparation technique of pesticide field, be specifically related to a kind of microbial pesticide preventing and treating apple pest and preparation method thereof.
Background technology
Apple is kind of a food low in calories, and every 100 grams only produce 60 kilocalories of heats；Apple Middle nutrition composition solubility is big, is easily absorbed by the body, therefore has the title of " running water ", be conducive to dissolving element sulphur, makes skin lubrication tender.Apple is containing materials such as abundant carbohydrates, mainly sucrose, reduced sugar and protein, fat, phosphorus, iron, potassium；Possibly together with malic acid, quininic acid, citric acid, tartaric acid, tannic acid, pectin, cellulose, B family vitamin, vitamin C and trace element edible portion 76%.
Though apple is of high nutritive value, but apple pest is the most, and existing agricultural chemicals is unsatisfactory to the insect killing effect of pest and disease damage.Such as: first, the insect such as leafhopper, plant hopper, aphid, mirid pentatomid, coccid etc. that apple is common, to apple injury greatly, orchard worker uses the agricultural chemicals of routine to carry out after spraying kills, and prevention effect is general；Second, corrode the common germ such as apple black skin shell bacterium of apple, shell capsule spore bacterium, dry rot of apple bacterium, pears raw capsule spore shell bacterium, GLOMERFLLA CINGULATA bacterium etc., apple production, quality is made to receive serious infringement, existing agricultural chemicals germ common to apple does not suppress, resistant function, antibacterial, antibacterial effect is poor；3rd, during preventing and treating pest and disease damage, big to apple plants and apple side effect, security is low；4th, the yield of apple is also affected by agricultural chemicals, and underproduction phenomenon is obvious, and apple Middle nutrition composition such as carbohydrate and pectin, the content of fructose also decrease simultaneously.
In summary, prior art there will naturally be inconvenience and defect in actual use, it is therefore necessary to improved.
Summary of the invention
For solving technical problem present in prior art, the present invention provides a kind of microbial pesticide preventing and treating apple pest and preparation method thereof, to realize following goal of the invention:
1, microbial pesticide prepared by the present invention is to the common insect of apple: leafhopper, plant hopper, aphid, mirid pentatomid, coccid prevention effect are good；
2, microbial pesticide prepared by present invention germ common to apple plants and apple, has antibacterial, antibacterial effect；
3, microbial pesticide prepared by the present invention, during the preventing and treating to apple, has no side effect to apple, and security is high.
4, microbial pesticide prepared by the present invention is while carrying out apple pest preventing and treating and Anti-bacterium growth, and the yield of apple is significantly improved, and in apple, pectin, the content of fructose also significantly improve.
For solving above-mentioned technical problem, the technical scheme that the present invention takes is as follows:
A kind of microbial pesticide preventing and treating apple pest, described microbial pesticide includes following components: Fenpropathrin, emulsifying agent, organic solvent, antifreezing agent, thickener, water, purple bergenia herb extract, Fluoxastrobin, Asiatic plantain water logging agent, chlorophyll.
The following is and the further of technique scheme is improved:
The component of described microbial pesticide, in parts by weight, content is:
Fenpropathrin 16～24 parts, emulsifying agent 11～19 parts, organic solvent 19～27 parts, antifreezing agent 1～3 parts, thickener 3～7 parts, water 21～29 parts, purple bergenia herb extract 2～6 parts, Fluoxastrobin 2～4 parts, Asiatic plantain water logging agent 1～3 parts, chlorophyll 1～3 parts.
The component of described microbial pesticide, in parts by weight, content is:
Fenpropathrin 20 parts, emulsifying agent 15 parts, organic solvent 23 parts, antifreezing agent 2 parts, thickener 5 parts, 25 parts of water, purple bergenia herb extract 4 parts, Fluoxastrobin 3 parts, Asiatic plantain water logging agent 2 parts, chlorophyll 2 parts.
Described emulsifying agent is Ben-zylphenol Polyoxyethyl Ether.
Described organic solvent is cyclohexanone.
Described antifreezing agent is glycerine.
Thick dose of described synergy is xanthans.
Described purple bergenia herb extract includes: Bergenin；Described Determination of Bergenin is 98%.
The preparation method of a kind of microbial pesticide preventing and treating apple pest, comprises the following steps:
(1) raw material prepares: take Fenpropathrin, Ben-zylphenol Polyoxyethyl Ether, cyclohexanone, glycerine, xanthans, water, purple bergenia herb extract, Fluoxastrobin, Asiatic plantain water logging agent and chlorophyll according to the weight ratio of above-described embodiment 1～3；
(2) mixing: put into by Fenpropathrin in cyclohexanone and dissolve 30 minutes, forms homogeneous oil phase；By Ben-zylphenol Polyoxyethyl Ether, glycerine, xanthans, water, purple bergenia herb extract, Fluoxastrobin, Asiatic plantain water logging agent and chlorophyll mix and blend 15 minutes, form homogeneous aqueous phase；Pour oil phase into aqueous phase, at 17 DEG C ± 2 DEG C, stir 10 minutes under rotating speed 1500～1700 revs/min, i.e. make the microbial pesticide of the present invention.
The extracting method of described purple bergenia herb extract comprises the following steps:
(1) purple bergenia herb is taken, by purple bergenia herb grind into fine powder, with 85% ethanol cold soaking 20h of 25 times amount；
(2) collect filtrate with Buchner funnel, be repeated 4 times；
(3) by filtrate with Rotary Evaporators decompression distillation, concentrated filtrate, concentrated filtrate low temperature drying, crude extract is obtained；
(4) crude extract is decoloured through macroporous resin adsorption, after 95% ethanol elution, eluent is concentrated and is evaporated, frozen drying, obtain purple bergenia herb extract.
Compared with prior art, the invention have the benefit that
1, microbial pesticide prepared by the present invention, after dispenser 30 days, the insect common to apple: leafhopper, plant hopper, aphid, mirid pentatomid, the prevention effect of coccid respectively reach 97.5～99.5%, 95.8～99.4%, 96.7～99.0%, 95.5～98.9%, 96.5～99.2%；
2, microbial pesticide prepared by the present invention, after dispenser 30 days, the germ common to apple: apple black skin shell bacterium, shell capsule spore bacterium, dry rot of apple bacterium, pears raw capsule spore shell bacterium, the suppression of GLOMERFLLA CINGULATA bacterium, resist effective, respectively reaches 94.6～97.5%, 95.2～97.4%, 96.3～97.8%, 95.4～97.9%, 94.3～97.6%；
3, microbial pesticide prepared by the present invention, during the preventing and treating to apple, has no side effect to apple, and security is high；
4, microbial pesticide prepared by present invention germ common to apple plants and apple, there is antibacterial, antibacterial effect, after dispenser 30 days, the germ common to apple: apple black skin shell bacterium, shell capsule spore bacterium, dry rot of apple bacterium, pears raw capsule spore shell bacterium, the suppression of GLOMERFLLA CINGULATA bacterium, resist effective, respectively reaches 94.6～97.5%, 95.2～97.4%, 96.3～97.8%, 95.4～97.9%, 94.3～97.6%.
Detailed description of the invention
Below by way of specific embodiment, the present invention is described, but the present invention is not merely defined in these embodiments.
Embodiment
1
A kind of microbial pesticide preventing and treating apple pest
Described microbial pesticide includes the component of following weight portion:
Fenpropathrin 16 parts, emulsifying agent 11 parts, organic solvent 19 parts, antifreezing agent 1 part, thickener 3 parts, 21 parts of water, purple bergenia herb extract 2 parts, Fluoxastrobin 2 parts, Asiatic plantain water logging agent 1 part, chlorophyll 1 part；
Described emulsifying agent is Ben-zylphenol Polyoxyethyl Ether；
Described organic solvent is cyclohexanone；
Described antifreezing agent is glycerine；
Thick dose of described synergy is xanthans；
Described purple bergenia herb extract includes: Bergenin；Described Determination of Bergenin is 98%.
Embodiment
2
A kind of microbial pesticide preventing and treating apple pest
Described microbial pesticide includes the component of following weight portion:
Fenpropathrin 20 parts, emulsifying agent 15 parts, organic solvent 23 parts, antifreezing agent 2 parts, thickener 5 parts, 25 parts of water, purple bergenia herb extract 4 parts, Fluoxastrobin 3 parts, Asiatic plantain water logging agent 2 parts, chlorophyll 2 parts；
Described emulsifying agent is Ben-zylphenol Polyoxyethyl Ether；
Described organic solvent is cyclohexanone；
Described antifreezing agent is glycerine；
Thick dose of described synergy is xanthans；
Described purple bergenia herb extract includes: Bergenin；Described Determination of Bergenin is 98%.
Embodiment
3
A kind of microbial pesticide preventing and treating apple pest
Described microbial pesticide includes the component of following weight portion:
Fenpropathrin 24 parts, emulsifying agent 19 parts, organic solvent 27 parts, antifreezing agent 3 parts, thickener 7 parts, 29 parts of water, purple bergenia herb extract 6 parts, Fluoxastrobin 4 parts, Asiatic plantain water logging agent 3 parts, chlorophyll 3 parts；
Described emulsifying agent is Ben-zylphenol Polyoxyethyl Ether；
Described organic solvent is cyclohexanone；
Described antifreezing agent is glycerine；
Thick dose of described synergy is xanthans；
Described purple bergenia herb extract includes: Bergenin；Described Determination of Bergenin is 98%.
Embodiment
4
A kind of preparation method of the microbial pesticide preventing and treating apple pest
The preparation method of a kind of microbial pesticide preventing and treating apple pest comprises the following steps:
(1) raw material prepares: take Fenpropathrin, Ben-zylphenol Polyoxyethyl Ether, cyclohexanone, glycerine, xanthans, water, purple bergenia herb extract, Fluoxastrobin, Asiatic plantain water logging agent and chlorophyll according to the weight ratio of above-described embodiment 1～3；
(2) mixing: put into by Fenpropathrin in cyclohexanone and dissolve 30 minutes, forms homogeneous oil phase；By Ben-zylphenol Polyoxyethyl Ether, glycerine, xanthans, water, purple bergenia herb extract, Fluoxastrobin, Asiatic plantain water logging agent and chlorophyll mix and blend 15 minutes, form homogeneous aqueous phase；Pour oil phase into aqueous phase, at 17 DEG C ± 2 DEG C, stir 10 minutes under rotating speed 1500～1700 revs/min, i.e. make the microbial pesticide of the present invention.
The extracting method of described purple bergenia herb extract comprises the following steps:
(1) purple bergenia herb is taken, by purple bergenia herb grind into fine powder, with 85% ethanol cold soaking 20h of 25 times amount；
(2) collect filtrate with Buchner funnel, be repeated 4 times；
(3) by filtrate with Rotary Evaporators decompression distillation, concentrated filtrate, concentrated filtrate low temperature drying, crude extract is obtained；
(4) crude extract is decoloured through macroporous resin adsorption, after 95% ethanol elution, eluent is concentrated and is evaporated, frozen drying, obtain purple bergenia herb extract.
Percentage in above-described embodiment, in addition to specified otherwise, for mass percent.
Result detects:
(1) microbial pesticide prepared by the present invention is good to the prevention effect of Apple Pests, after dispenser 30 days, the insect common to apple: leafhopper, plant hopper, aphid, mirid pentatomid, the prevention effect of coccid respectively reach 97.5～99.5%, 95.8～99.4%, 96.7～99.0%, 95.5～98.9%, 96.5～99.2%, and specific targets are shown in Table 1；
Microbial pesticide prepared by table 1 present invention prevention effect to apple common insect pests
(2) microbial pesticide prepared by present invention germ common to apple plants and apple, there is antibacterial, antibacterial effect, after dispenser 30 days, the germ common to apple: apple black skin shell bacterium, shell capsule spore bacterium, dry rot of apple bacterium, pears raw capsule spore shell bacterium, the suppression of GLOMERFLLA CINGULATA bacterium, resist effective, respectively reaching 94.6～97.5%, 95.2～97.4%, 96.3～97.8%, 95.4～97.9%, 94.3～97.6%, specific targets are shown in Table 2；
The suppression of microbial pesticide germ common to apple prepared by table 2 present invention, opposing effect
(3) microbial pesticide prepared by the present invention, during the preventing and treating to apple, has no side effect to apple plants and apple, and security is high；
(4) microbial pesticide prepared by the present invention is while carrying out apple pest preventing and treating and Anti-bacterium growth, the yield of apple is significantly improved, and the content of carbohydrate and pectin, fructose also significantly improves in apple, the per mu yield of apple reaches 5500 jin～6600 jin, and in apple, the content of carbohydrate and pectin reaches 18.7
G/100g～25.0 g/100g, in apple, content 11.0g/100g～16.2g/100g of fructose, is specifically shown in Table 3；
Per mu yield, carbohydrate and the pectin of table 3 apple, the content of fructose
Comparison: do not use microbial pesticide of the present invention, replaces microbial pesticide to spray with clear water.
Last it is noted that the foregoing is only the preferred embodiments of the present invention, it is not limited to the present invention, although the present invention being described in detail with reference to previous embodiment, for a person skilled in the art, technical scheme described in foregoing embodiments still can be modified by it, or wherein portion of techniques feature is carried out equivalent.All within the spirit and principles in the present invention, any modification, equivalent substitution and improvement etc. made, should be included within the scope of the present invention.
Claims (10)
1. the microbial pesticide preventing and treating apple pest, it is characterised in that: described microbial pesticide includes following components:
Fenpropathrin, emulsifying agent, organic solvent, antifreezing agent, thickener, water, purple bergenia herb extract, Fluoxastrobin, Asiatic plantain water logging agent, chlorophyll.
A kind of microbial pesticide preventing and treating apple pest the most according to claim 1, it is characterised in that: the component of described microbial pesticide, in parts by weight, content is:
Fenpropathrin 16～24 parts, emulsifying agent 11～19 parts, organic solvent 19～27 parts, antifreezing agent 1～3 parts, thickener 3～7 parts, water 21～29 parts, purple bergenia herb extract 2～6 parts, Fluoxastrobin 2～4 parts, Asiatic plantain water logging agent 1～3 parts, chlorophyll 1～3 parts.
A kind of microbial pesticide preventing and treating apple pest the most according to claim 1, it is characterised in that: the component of described microbial pesticide, in parts by weight, content is:
Fenpropathrin 20 parts, emulsifying agent 15 parts, organic solvent 23 parts, antifreezing agent 2 parts, thickener 5 parts, 25 parts of water, purple bergenia herb extract 4 parts, Fluoxastrobin 3 parts, Asiatic plantain water logging agent 2 parts, chlorophyll 2 parts.
A kind of microbial pesticide preventing and treating apple pest the most according to claim 1, it is characterised in that: described emulsifying agent is Ben-zylphenol Polyoxyethyl Ether.
A kind of microbial pesticide preventing and treating apple pest the most according to claim 1, it is characterised in that: described organic solvent is cyclohexanone.
A kind of microbial pesticide preventing and treating apple pest the most according to claim 1, it is characterised in that: described antifreezing agent is glycerine.
A kind of microbial pesticide preventing and treating apple pest the most according to claim 1, it is characterised in that: thick dose of described synergy is xanthans.
A kind of microbial pesticide preventing and treating apple pest the most according to claim 1, it is characterised in that: described purple bergenia herb extract includes: Bergenin；Described Determination of Bergenin is 98%.
9. the preparation method of the microbial pesticide preventing and treating apple pest, it is characterised in that: comprise the following steps:
(1) raw material prepares: take Fenpropathrin, Ben-zylphenol Polyoxyethyl Ether, cyclohexanone, glycerine, xanthans, water, purple bergenia herb extract, Fluoxastrobin, Asiatic plantain water logging agent and chlorophyll according to the weight ratio of above-described embodiment 1～3；
(2) mixing: put into by Fenpropathrin in cyclohexanone and dissolve 30 minutes, forms homogeneous oil phase；By Ben-zylphenol Polyoxyethyl Ether, glycerine, xanthans, water, purple bergenia herb extract, Fluoxastrobin, Asiatic plantain water logging agent and chlorophyll mix and blend 15 minutes, form homogeneous aqueous phase；Pour oil phase into aqueous phase, at 17 DEG C ± 2 DEG C, stir 10 minutes under rotating speed 1500～1700 revs/min, i.e. make the microbial pesticide of the present invention.
The preparation method of a kind of microbial pesticide preventing and treating apple pest the most according to claim 9, it is characterised in that:
The extracting method of described purple bergenia herb extract comprises the following steps:
(1) purple bergenia herb is taken, by purple bergenia herb grind into fine powder, with 85% ethanol of 25 times amount
Cold soaking 20h；
(2) collect filtrate with Buchner funnel, be repeated 4 times；
(3) by filtrate with Rotary Evaporators decompression distillation, concentrated filtrate, concentrated filtrate low temperature drying, crude extract is obtained；
(4) crude extract is decoloured through macroporous resin adsorption, after 95% ethanol elution, eluent is concentrated and is evaporated, frozen drying, obtain purple bergenia herb extract.
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Cited By (2)
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| CN108033850A (en) * | 2017-12-13 | 2018-05-15 | 河北燊垚生物科技有限公司 | Prevent the liquid fertilizer of plant pest |
| CN114794130A (en) * | 2021-06-21 | 2022-07-29 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Control agent for macadimia nut anthracnose |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108033850A (en) * | 2017-12-13 | 2018-05-15 | 河北燊垚生物科技有限公司 | Prevent the liquid fertilizer of plant pest |
| CN114794130A (en) * | 2021-06-21 | 2022-07-29 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Control agent for macadimia nut anthracnose |
| CN114794130B (en) * | 2021-06-21 | 2023-09-26 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Agent for preventing and treating anthracnose of macadimia nut |
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Application publication date: 20160810 |