CN113396845A - Method for improving survival rate of two-year-old patinopecten yessoensis cultured in raft - Google Patents

Method for improving survival rate of two-year-old patinopecten yessoensis cultured in raft Download PDF

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CN113396845A
CN113396845A CN202110834637.0A CN202110834637A CN113396845A CN 113396845 A CN113396845 A CN 113396845A CN 202110834637 A CN202110834637 A CN 202110834637A CN 113396845 A CN113396845 A CN 113396845A
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bait
release
slow
patinopecten yessoensis
release block
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宋伦
毕相东
宋广军
吴金浩
孙明
王召会
王昆
杜静
李楠
刘桂英
李爱
胡超魁
田金
邵泽伟
杨爽
李安龙
赵海勃
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LIAONING OCEAN AND FISHERIES SCIENCE RESEARCH INSTITUTE
Tianjin Agricultural University
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LIAONING OCEAN AND FISHERIES SCIENCE RESEARCH INSTITUTE
Tianjin Agricultural University
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Publication of CN113396845A publication Critical patent/CN113396845A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/50Culture of aquatic animals of shellfish
    • A01K61/54Culture of aquatic animals of shellfish of bivalves, e.g. oysters or mussels
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/60Floating cultivation devices, e.g. rafts or floating fish-farms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/163Sugars; Polysaccharides
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/26Compounds containing phosphorus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K40/00Shaping or working-up of animal feeding-stuffs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures

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  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Microbiology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Biomedical Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Physiology (AREA)
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  • Feed For Specific Animals (AREA)
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  • Fodder In General (AREA)

Abstract

The invention discloses a method for improving the survival rate of two-year-old patinopecten yessoensis cultured in raft, belonging to the technical field of shellfish culture, and the method utilizes exogenous nutrition input to increase the immunity and growth rate of the patinopecten yessoensis and improve the survival rate of the patinopecten yessoensis; exogenous input nutrition is implemented by adopting a bait slow-release block, and the bait slow-release block comprises 150-250:3-7:30-40:50-70 mass ratio of dunaliella salina powder, lactic acid bacteria, inorganic salt and corn starch, wherein the inorganic salt is inorganic salt containing nitrogen, phosphorus and silicon elements, and the mass ratio of the nitrogen elements, the phosphorus elements and the silicon elements is 27-37:1: 2; the invention has no pollution to the ecological environment, is beneficial and harmless to the growth of cultured shellfish, and the bait slow-release block can slowly release nutrient salt and bait in seawater, thereby increasing beneficial bait microalgae in the seawater and improving the culture benefit.

Description

Method for improving survival rate of two-year-old patinopecten yessoensis cultured in raft
Technical Field
The invention relates to the technical field of shellfish culture, in particular to a method for improving the survival rate of patinopecten yessoensis cultured in raft for two ages.
Background
Patinopecten yessoensis (Patinopecten yessoensis) belongs to the phylum Mollusca (Mollusca), the class of lamprex (Lamellibranchia), the class of pteridophyceae (Pterimorphia), the order of Pterioda (Pterioodae) and the family of Pectinidae, and has the advantages of large adductor muscle, delicious taste, high nutritional value and good economic benefit, thus being deeply welcomed by consumers and culture units. Patinopecten yessoensis belongs to filter-feeding bivalve shellfish, and has fan-shaped shell, more prominent right shell, yellowish white color, slightly flat left shell and slightly smaller right shell, and purple brown color. The surface of the shell is provided with 15-20 radial ribs, and the shell ears at two sides are provided with shallow byssus holes. The right shell is wide and low in rib width and narrow in intercostal space; the left shell rib is thinner and the ribs are wider. A triangular inner ligament is arranged below the top of the shell. Naturally distributed at the water depth of 6-60 m, and the bottom is gravel. The Japanese scallop is a cold water shellfish and grows at a proper temperature range of 5-23 ℃. Originally, the method is mainly produced in the south water areas of Japan and Russian thousand island islands, the North sea of Japan and the North China. The salinity is within a proper range of 24-40, and the distribution is high in salinity, the bottom material without fresh water injection is hard, the silt is less, and the water depth is not more than 40m in coastal sea areas. Mainly used for cultivation in the sea areas such as Liaodong peninsula and Shandong Changdai in the north of China. The growth speed is slow, 1l-12 cm from the young shellfish to the shell, and the shortest time is 1 year and 7 months. It is stated that the maximum shell height can be as high as 27.94 cm, with a lifetime of about 25 years.
In recent years, the phenomenon of quality reduction, even large-scale death, such as long stagnation, low fertilizer fullness, small specification and the like, of seaculture shellfish generally appears, and becomes an important factor for limiting the sustainable development of marine fishery. At present, the patinopecten yessoensis culture is still 'eaten by the day', and the patinopecten yessoensis grows by eating natural microalgae bait. In nearly 10 years, the death rate of two-year-old patinopecten yessoensis cultured in raft in Changhai county is over 80-90%, so that the two-year-old patinopecten yessoensis is not cultured in raft basically at present, and the scale is less than 0.1% before 10 years. Bait microalgae is an energy basis and health guarantee for bivalve shellfish, the evolution of the species, the quantity and the grain size of the bait microalgae has great influence on the healthy growth and survival of the bivalve shellfish, the microalgae with small grain size and low nutrition are not beneficial to the nutritional storage and healthy growth of the bivalve shellfish, earlier researches find that the continuous expansion of the sea area culture scale in Changhai county, the reduction of nutritional factors such as nitrogen and phosphorus in culture areas and climate change lead to the positive trend of the grain size of the microalgae towards miniaturization, and the large-grain microalgae occupies less area, thus causing the low immunity and large-scale death of the Japanese scallop.
Disclosure of Invention
The invention aims to provide a method for improving the survival rate of patinopecten yessoensis cultured in two ages in raft, which solves the problem of the lack of beneficial bait in a patinopecten yessoensis culture area through exogenous bait supplement, enhances the immunity and growth rate of the patinopecten yessoensis, improves the survival rate and promotes the healthy and sustainable development of shellfish culture industry.
In order to achieve the purpose, the invention provides the following scheme:
the invention provides a bait slow-release block for Japanese scallops, which comprises 150-weight ratio of 250:3-7:30-40:50-70 of dunaliella salina powder, lactic acid bacteria, inorganic salt and corn starch.
Further, the inorganic salt is an inorganic salt containing nitrogen, phosphorus and silicon elements, wherein the mass ratio of the nitrogen elements to the phosphorus elements to the silicon elements is 27-37:1: 2.
Further, the inorganic salts include sodium nitrate, sodium dihydrogen phosphate, and sodium silicate.
Furthermore, the bait slow-release block is blocky and has the mass of 50-500 g.
The invention also provides a preparation method of the bait slow-release block for the comb shells, which comprises the following steps:
and uniformly mixing the dunaliella salina powder, the corn starch, the inorganic salt and the lactic acid bacteria, adding water, and pressing into blocks to obtain the bait slow-release block of the patinopecten yessoensis.
The invention also provides a comb shell breeding raft, which comprises 200 suspension cages, wherein each suspension cage is suspended with 3 bait slow-release bags, each bait slow-release bag contains 4 bait slow-release blocks, and the bait slow-release bags are suspended at the upper part, the middle part and the lower part of the outer side of the suspension cage.
The invention also provides application of the bait slow-release block in improving the survival rate of the comb shells cultured in the raft.
The invention also provides a method for improving the survival rate of two-year-old patinopecten yessoensis cultured in raft, which utilizes the exogenous nutrition input to increase the immunity and growth rate of the patinopecten yessoensis and improve the survival rate of the patinopecten yessoensis; wherein, the exogenous input nutrition comprises the bait slow-release block.
Further, the bait slow-release blocks are placed in a 100-mesh net bag to form bait slow-release bags, and the bait slow-release bags are respectively hung on the upper portion, the middle portion and the lower portion of the outer side of the suspension cage.
Further, the bait slow-release block is replaced when the comb shells are poured into the cage, and the use condition of the bait slow-release block is that the water temperature is below 15 ℃.
The invention discloses the following technical effects:
the invention provides a method for improving the survival rate of two-year-old patinopecten yessoensis cultured in raft, which increases the immunity and growth rate of the patinopecten yessoensis by using exogenous nutrition input and slow release modes, thereby improving the survival rate of the patinopecten yessoensis.
At present, no method for improving the survival rate of the shellfish in a natural sea area exists, the method has no pollution to the ecological environment, is beneficial and harmless to the growth of the cultured shellfish, and the bait slow-release block can slowly release nutrient salt and bait in seawater, so that beneficial bait microalgae in the seawater are increased, and the culture benefit is improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 is a schematic diagram of a test deployment and sampling station;
FIG. 2 is a graph showing the comparison of the survival rate of Japanese scallop for 7 months;
FIG. 3 is a comparison graph of 7-month single-weight test of Japanese scallop;
fig. 4 is a comparison graph of a survival subject (left side) and a death subject (right side) of Japanese scallop;
fig. 5 is a comparison of a test area (left side) and a control area (right side) of Japanese scallop.
Detailed Description
Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but as a more detailed description of certain aspects, features and embodiments of the invention.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. Further, for numerical ranges in this disclosure, it is understood that each intervening value, between the upper and lower limit of that range, is also specifically disclosed. Every smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference herein for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. The description and examples are intended to be illustrative only.
As used herein, the terms "comprising," "including," "having," "containing," and the like are open-ended terms that mean including, but not limited to.
Example 1
A bait slow-release block for improving the survival rate of two-year-old patinopecten yessoensis cultured in raft is prepared by the following steps: 200g of dunaliella salina powder, 60g of corn starch, 33g of sodium nitrate, 1g of sodium dihydrogen phosphate, 2g of sodium silicate and 5g of lactic acid bacteria, then 10mL of water is added, the mixture is uniformly stirred, and finally the mixture is pressed by a tablet press to obtain the block-shaped bait slow-release block. 2400 identical bait slow-release blocks are prepared according to the same method and are placed in 100-mesh net bags, and 4 bait slow-release blocks are placed in each net bag to form 1 bait slow-release bag.
Example 2
A bait slow-release block for improving the survival rate of two-year-old patinopecten yessoensis cultured in raft is prepared by the following steps: 150g of dunaliella salina powder, 50g of corn starch, 27g of sodium nitrate, 1g of sodium dihydrogen phosphate, 2g of sodium silicate and 7g of lactic acid bacteria, then 10mL of water is added, the mixture is stirred uniformly, and finally the mixture is pressed by a tablet press to obtain the block-shaped bait slow-release block. 2400 identical bait slow-release blocks are prepared according to the same method and are placed in 100-mesh net bags, and 4 bait slow-release blocks are placed in each net bag to form 1 bait slow-release bag.
Example 3
A bait slow-release block for improving the survival rate of two-year-old patinopecten yessoensis cultured in raft is prepared by the following steps: 250g of dunaliella salina powder, 70g of corn starch, 37g of sodium nitrate, 1g of sodium dihydrogen phosphate, 2g of sodium silicate and 3g of lactic acid bacteria, then 10mL of water is added, the mixture is stirred uniformly, and finally the mixture is pressed by a tablet press to obtain the block-shaped bait slow-release block. 2400 identical bait slow-release blocks are prepared according to the same method and are placed in 100-mesh net bags, and 4 bait slow-release blocks are placed in each net bag to form 1 bait slow-release bag.
Test examples
In 16 days 9 and 2020, in the small Changshan island Yingjicun patinopecten yessoensis floating raft culture sea area in DaLianghai county, 20 mu of test area 2 floating rafts are selected to hang the bait slow-release blocks of the embodiment 1, each cage is hung with 3 slow-release bags and respectively fixed at the upper, middle and lower positions outside the cage, each floating raft has 200 cages, 600 bait slow-release bags (2400 bait slow-release blocks) are hung, meanwhile, in the area outside 100 meters, 2 floating rafts are selected as the reference, and the test layout is shown as fig. 1.
By 2021 year, 4 months and 21 days, the survival rate of the second-instar patinopecten yessoensis in the test area is averagely improved by 10 percent (figure 2), and the weight is improved by 18.88 percent (figure 3).
The patinopecten yessoensis survival rate can be obviously improved by the suspended bait slow-release block indirectly proved by the fact that the patinopecten yessoensis survival rate is not dead basically in the test period (in figure 4, the left side in the figure is a survival individual, and the right side in the figure is a dead individual in the early stage of the patinopecten yessoensis test (9 months in 2020)).
The living condition of Patinopecten yessoensis in the test area and the control area is investigated on 21/4/2021 (fig. 5, the left side is the test area, and the right side is the control area), and it is obvious that the size of the individual scallop in the left test area is larger than that in the right control area.
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.

Claims (10)

1. The bait slow-release block for the comb shells is characterized by comprising 150-250:3-7:30-40:50-70 mass ratio of saline algae powder, lactic acid bacteria, inorganic salt and corn starch.
2. The bait sustained-release block for patinopecten yessoensis according to claim 1, wherein the inorganic salt is an inorganic salt containing nitrogen, phosphorus and silicon elements, wherein the mass ratio of the nitrogen element, the phosphorus element and the silicon element is 27-37:1: 2.
3. The bait sustained-release block for patinopecten yessoensis according to claim 2, wherein the inorganic salt comprises sodium nitrate, sodium dihydrogen phosphate and sodium silicate.
4. The bait slow-release block for patinopecten yessoensis according to claim 1, wherein the bait slow-release block is block-shaped, and the mass of the block-shaped bait slow-release block is 50-500 g.
5. A method for preparing a bait sustained-release block of Japanese scallop as claimed in any one of claims 1 to 4, which comprises the following steps:
and uniformly mixing the dunaliella salina powder, the corn starch, the inorganic salt and the lactic acid bacteria, adding water, and pressing into blocks to obtain the bait slow-release block of the patinopecten yessoensis.
6. A comb shell breeding raft, characterized in that the raft comprises 200 suspension cages, 3 bait slow-release bags are suspended on each suspension cage, each bait slow-release bag contains 4 bait slow-release blocks as claimed in any one of claims 1 to 4, and the bait slow-release bags are suspended at the upper part, the middle part and the lower part of the outer side of the suspension cage.
7. Use of the bait slow-release block as claimed in any one of claims 1 to 4 for improving the survival rate of the patinopecten yessoensis cultured in the raft.
8. A method for improving the survival rate of two-year-old patinopecten yessoensis cultured in raft is characterized in that exogenous nutrition is utilized to input, so that the immunity and growth rate of the patinopecten yessoensis are improved, and the survival rate of the patinopecten yessoensis is improved; wherein the exogenous input nutrition comprises the bait sustained-release block as claimed in any one of claims 1 to 4.
9. The method of claim 8, wherein the bait slow-release block is placed in a 100-mesh net bag to form bait slow-release bags, and the bait slow-release bags are respectively hung at the upper part, the middle part and the lower part of the outer side of the suspension cage.
10. The method as claimed in claim 8, wherein the bait slow-release block is replaced when the comb shell is poured into the cage, and the use condition of the bait slow-release block is that the water temperature is below 15 ℃.
CN202110834637.0A 2021-07-23 2021-07-23 Method for improving survival rate of two-year-old patinopecten yessoensis cultured in raft Pending CN113396845A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
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CN116082085A (en) * 2023-02-20 2023-05-09 辽宁省海洋水产科学研究院(辽宁省农业科学院大连生物技术研究所、辽宁省海洋环境监测总站) Sustained-release gel silicon fertilizer for improving shellfish survival rate and preparation method thereof
CN116262672A (en) * 2023-02-20 2023-06-16 辽宁省海洋水产科学研究院(辽宁省农业科学院大连生物技术研究所、辽宁省海洋环境监测总站) Preparation method of sodium alginate composite slow-release phosphate fertilizer, product and application thereof
CN116283420A (en) * 2023-02-20 2023-06-23 辽宁省海洋水产科学研究院(辽宁省农业科学院大连生物技术研究所、辽宁省海洋环境监测总站) Preparation method of composite slow-release nitrogenous fertilizer for improving feeding force of shellfish culture baits
CN117426338A (en) * 2023-12-22 2024-01-23 三亚屿礁海洋科技开发有限公司 Method for long-acting maintenance of plankton number based on aquaculture water

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US5567221A (en) * 1995-01-03 1996-10-22 Oms Investments, Inc. Compositions and methods for use in aquaculture
JPH1175A (en) * 1997-04-18 1999-01-06 Yoshitomi Fine Chem Kk Nutrient agent for shellfishes
ES2327586A1 (en) * 2006-03-08 2009-10-30 Juan Ignacio Rodriguez Vazquez Polygonal batea for bivalve crossing with all occupation surface dedicated to cultivation. (Machine-translation by Google Translate, not legally binding)
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CN116082085A (en) * 2023-02-20 2023-05-09 辽宁省海洋水产科学研究院(辽宁省农业科学院大连生物技术研究所、辽宁省海洋环境监测总站) Sustained-release gel silicon fertilizer for improving shellfish survival rate and preparation method thereof
CN116262672A (en) * 2023-02-20 2023-06-16 辽宁省海洋水产科学研究院(辽宁省农业科学院大连生物技术研究所、辽宁省海洋环境监测总站) Preparation method of sodium alginate composite slow-release phosphate fertilizer, product and application thereof
CN116283420A (en) * 2023-02-20 2023-06-23 辽宁省海洋水产科学研究院(辽宁省农业科学院大连生物技术研究所、辽宁省海洋环境监测总站) Preparation method of composite slow-release nitrogenous fertilizer for improving feeding force of shellfish culture baits
CN116082085B (en) * 2023-02-20 2023-08-15 辽宁省海洋水产科学研究院(辽宁省农业科学院大连生物技术研究所、辽宁省海洋环境监测总站) Sustained-release gel silicon fertilizer for improving shellfish survival rate and preparation method thereof
CN116262672B (en) * 2023-02-20 2023-10-27 辽宁省海洋水产科学研究院(辽宁省农业科学院大连生物技术研究所、辽宁省海洋环境监测总站) Preparation method of sodium alginate composite slow-release phosphate fertilizer, product and application thereof
CN116283420B (en) * 2023-02-20 2024-01-26 辽宁省海洋水产科学研究院(辽宁省农业科学院大连生物技术研究所、辽宁省海洋环境监测总站) Preparation method of composite slow-release nitrogenous fertilizer for improving feeding force of shellfish culture baits
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