CN111248123A - Fertilizer water paste for seawater shellfish pond culture and use method thereof - Google Patents
Fertilizer water paste for seawater shellfish pond culture and use method thereof Download PDFInfo
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- CN111248123A CN111248123A CN202010198445.0A CN202010198445A CN111248123A CN 111248123 A CN111248123 A CN 111248123A CN 202010198445 A CN202010198445 A CN 202010198445A CN 111248123 A CN111248123 A CN 111248123A
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 title claims abstract description 57
- 235000015170 shellfish Nutrition 0.000 title claims abstract description 36
- 239000013535 sea water Substances 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 10
- 239000003337 fertilizer Substances 0.000 title abstract description 11
- 241000192700 Cyanobacteria Species 0.000 claims abstract description 35
- 239000000843 powder Substances 0.000 claims abstract description 12
- 235000013619 trace mineral Nutrition 0.000 claims abstract description 8
- 239000011573 trace mineral Substances 0.000 claims abstract description 8
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 claims abstract description 6
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 6
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 6
- 239000004021 humic acid Substances 0.000 claims abstract description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 16
- 235000020639 clam Nutrition 0.000 claims description 14
- SRUWWOSWHXIIIA-UKPGNTDSSA-N Cyanoginosin Chemical compound N1C(=O)[C@H](CCCN=C(N)N)NC(=O)[C@@H](C)[C@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)C(=C)N(C)C(=O)CC[C@H](C(O)=O)N(C)C(=O)[C@@H](C)[C@@H]1\C=C\C(\C)=C\[C@H](C)[C@@H](O)CC1=CC=CC=C1 SRUWWOSWHXIIIA-UKPGNTDSSA-N 0.000 claims description 8
- 108010067094 microcystin Proteins 0.000 claims description 8
- 241000620877 Ruditapes philippinarum Species 0.000 claims description 5
- 241000237502 Ostreidae Species 0.000 claims description 3
- 235000020636 oyster Nutrition 0.000 claims description 3
- 241000192710 Microcystis aeruginosa Species 0.000 claims 2
- 241000195493 Cryptophyta Species 0.000 abstract description 7
- 150000001413 amino acids Chemical class 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 3
- 235000001014 amino acid Nutrition 0.000 abstract description 2
- 238000012258 culturing Methods 0.000 abstract description 2
- 239000006041 probiotic Substances 0.000 abstract description 2
- 235000018291 probiotics Nutrition 0.000 abstract description 2
- 230000035755 proliferation Effects 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract description 2
- 239000000047 product Substances 0.000 description 19
- 238000009360 aquaculture Methods 0.000 description 10
- 244000144974 aquaculture Species 0.000 description 10
- 241000894007 species Species 0.000 description 8
- 241000425347 Phyla <beetle> Species 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 230000004720 fertilization Effects 0.000 description 4
- 241000237519 Bivalvia Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000012544 monitoring process Methods 0.000 description 3
- 241000206761 Bacillariophyta Species 0.000 description 2
- 241000195628 Chlorophyta Species 0.000 description 2
- 241000206751 Chrysophyceae Species 0.000 description 2
- 241000514743 Cyclina sinensis Species 0.000 description 2
- 241000199914 Dinophyceae Species 0.000 description 2
- 241000195620 Euglena Species 0.000 description 2
- 241000287828 Gallus gallus Species 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000005611 electricity Effects 0.000 description 2
- 210000003608 fece Anatomy 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 239000010871 livestock manure Substances 0.000 description 2
- 239000003895 organic fertilizer Substances 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 239000011257 shell material Substances 0.000 description 2
- 239000010802 sludge Substances 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- 241000195649 Chlorella <Chlorellales> Species 0.000 description 1
- 108010049746 Microcystins Proteins 0.000 description 1
- 241000237503 Pectinidae Species 0.000 description 1
- 241001115039 Ruditapes Species 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000000446 fuel Substances 0.000 description 1
- 230000034659 glycolysis Effects 0.000 description 1
- 231100000784 hepatotoxin Toxicity 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 238000009342 intercropping Methods 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012982 microporous membrane Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000008239 natural water Substances 0.000 description 1
- 230000003472 neutralizing effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000011155 quantitative monitoring Methods 0.000 description 1
- 230000036186 satiety Effects 0.000 description 1
- 235000019627 satiety Nutrition 0.000 description 1
- 235000020637 scallop Nutrition 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000002344 surface layer Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 108700012359 toxins Proteins 0.000 description 1
- 238000003828 vacuum filtration Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
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- Bioinformatics & Cheminformatics (AREA)
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- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Botany (AREA)
- Biomedical Technology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Cell Biology (AREA)
- Animal Husbandry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a water and fertilizer paste for seawater shellfish pond culture and a using method thereof, wherein microalgae acidolysis products, humic acid, shell powder, bacillus subtilis powder and feeding trace elements are mixed and stirred uniformly according to the mass fraction of 50-70: 10-20: 4-6: 3-3.8: 0.1-0.2 to prepare a paste, and the water and fertilizer paste can be diluted according to the dosage of 1-1.5 kg/mu and then sprinkled for use when the transparency of a seawater shellfish culture pond is higher than 40 cm. The fertilizer water paste is prepared by using the bloom-forming cyanobacteria from the eutrophic water environment as a main raw material, and the obtained fertilizer water paste not only has strong affinity to the water environment, but also contains a large amount of active biological peptide, free amino acid, probiotics and trace elements necessary for the proliferation of microalgae. The invention can rapidly promote the beneficial algae in the culture pond to proliferate in large quantity, not only enables the water quality of the culture pond to present an obvious 'fertile, alive, tender and cool' state, but also can provide a large amount of fresh and alive microalgae baits for culturing shellfish, thereby achieving the purposes of increasing both production and income.
Description
Technical Field
The invention relates to the technical field of aquaculture, in particular to a water fertilizing paste for seawater shellfish pond culture and a using method thereof.
Background
The seawater shellfish culture has the highest yield in the aquaculture of China and great overall economic benefit, so the phenomenon of overhigh stocking density of cultured shellfish is particularly prominent. Because most cultured shellfish (scallops, clams, oysters, ruditapes philippinarum and the like) depend on phytoplankton in the filter-feeding aquaculture water environment to live, the density of the phytoplankton in the water environment can be directly and rapidly reduced when the shellfish stocking density in a relatively closed seawater aquaculture pond is too high, so that the density is far lower than the daily satiety requirement of the cultured shellfish, and the specification of the cultured shellfish is directly reduced, and the aquaculture yield is reduced. At present, in daily aquaculture production, in order to increase the density of phytoplankton in a seawater shellfish pond, farmers often use inorganic fertilizer or chicken manure fermented material rich water, but the rich water effect of the inorganic fertilizer is not lasting, and the fermented chicken manure rich water is easy to spoil the water quality of the pond, so that the seawater shellfish aquaculture pond urgently needs products with good rich water effect and high environmental safety.
Eutrophication causes frequent occurrence of cyanobacterial bloom in natural water body in China, the current treatment measures of the blue algae in the lake are taken as representatives, and the resource utilization practice of the cyanobacterial bloom in China is mainly to generate electricity by using the cyanobacterial as a biomass fuel; however, the direct incineration of the cyanobacteria sludge to generate electricity causes great waste of cyanobacteria protein resources, because the protein content of the cyanobacteria sludge can reach more than 40%. However, the bloom-forming cyanobacteria contains primary hepatotoxin, namely microcystin, so that the wide resource utilization of the bloom-forming cyanobacteria is limited. Therefore, if a large amount of bioactive peptides, free amino acids and the like can be obtained by acidolysis of cyanobacteria algae mud, microcystin contained in the cyanobacteria algae mud is degraded to be below the safe concentration and is used for seawater shellfish culture, a new low-cost, high-efficiency and high-valued resource utilization mode of the bloom-forming cyanobacteria is developed, so that the harm is really changed into treasure, the additional value of the resource utilization of the bloom-forming cyanobacteria is improved, and more social resources are promoted to participate in prevention and control of the cyanobacteria bloom.
Disclosure of Invention
The technical problem to be solved by the invention is to provide the water fertilizing paste for the pond culture of the seawater shellfish and the using method thereof, and the water fertilizing paste can be applied to daily water fertilizing operation of pond culture of the seawater shellfish such as the clams, the oysters, the Ruditapes philippinarum and the like.
In order to solve the technical problems, the invention adopts the technical scheme that: a water fertilizing paste for seawater shellfish pond culture is a paste prepared by mixing and stirring microalgae acidolysis product, humic acid, shell powder, bacillus subtilis powder and feeding trace elements according to the mass fraction of 50-70: 10-20: 4-6: 3-3.8: 0.1-0.2; the microalgae acidolysis product is a bloom-forming cyanobacteria acidolysis product, and the content of microcystin in dry matter is lower than 2.0 micrograms/kg.
The acidolysis product of the bloom-forming cyanobacteria is prepared by using concentrated hydrochloric acid to acidolyze bloom-forming cyanobacteria mud with the water content of 70-90%, wherein the volume of the concentrated hydrochloric acid is as follows: the volume of the cyanobacteria bloom mud is 15-30%.
When the transparency of the seawater shellfish culture pond is higher than 40 cm, the seawater shellfish culture pond is diluted according to the dosage of 1-1.5 kg/mu and then sprinkled for use.
The seawater shellfish is Cyclina sinensis, Concha Ostreae and Ruditapes philippinarum.
The invention has the beneficial effects that: the invention uses the bloom-forming cyanobacteria from the eutrophic water environment as the main raw material to prepare the fertilizer-water paste, which not only has strong affinity to the water environment, but also contains a large amount of active biological peptide, free amino acid, probiotics and trace elements necessary for the proliferation of the microalgae. Can quickly promote the beneficial algae in the culture pond to proliferate in a large quantity, not only enables the water quality of the culture pond to present an obvious 'fertile, alive, tender and cool' state, but also can provide a large quantity of fresh and alive microalgae baits for culturing shellfish so as to achieve the purposes of increasing both production and income.
Detailed Description
The technical scheme in the embodiment of the invention will be clearly and completely described below in combination with the embodiment of the invention; it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by those skilled in the art without any inventive work are within the scope of the present invention.
The water fertilizing paste for seawater shellfish pond culture is a paste prepared by mixing and stirring microalgae acidolysis product, humic acid, shell powder, bacillus subtilis powder and feeding trace elements according to the mass fraction of 50-70: 10-20: 4-6: 3-3.8: 0.1-0.2; the microalgae acidolysis product is a bloom-forming cyanobacteria acidolysis product, and the content of microcystin in dry matter is lower than 2.0 micrograms/kg.
The acidolysis product of the bloom-forming cyanobacteria is prepared by using concentrated hydrochloric acid to acidolyze bloom-forming cyanobacteria mud with the water content of 70-90%, wherein the volume of the concentrated hydrochloric acid is as follows: the volume of the cyanobacteria bloom mud is 15-30%.
When the transparency of the seawater shellfish culture pond is higher than 40 cm, the seawater shellfish culture pond is diluted according to the dosage of 1-1.5 kg/mu and then sprinkled for use.
The seawater shellfish is Cyclina sinensis, Concha Ostreae and Ruditapes philippinarum.
Example 1
20 kg of humic acid, 6 kg of shell powder, 3.8 kg of bacillus subtilis powder and 0.2 kg of feeding trace elements are added into 70 kg of water-blooming cyanobacteria acidolysis product (prepared in example 3), and then the mixture is uniformly stirred by an organic fertilizer mixing stirrer to prepare 100 kg of paste, namely the seawater shellfish culture pond fertilizer water paste.
Example 2
13 kg of humic acid, 5 kg of shell powder, 3.8 kg of bacillus subtilis powder and 0.2 kg of feeding trace elements are added into 58 kg of water-blooming cyanobacteria acidolysis product (prepared in example 3), and then the mixture is uniformly stirred by an organic fertilizer mixer to prepare 80 kg of paste, namely the seawater shellfish culture pond fertilizer water paste.
Example 3
1) Putting the bloom-forming cyanobacteria mud with the water content of 86.4 percent into a 50-liter reaction kettle, adding 20 percent hydrochloric acid (volume of a commercially available concentrated hydrochloric acid/volume of the bloom-forming cyanobacteria mud) according to volume, carrying out acidolysis for 8 hours at 120 ℃, and then neutralizing by using sodium hydroxide (NaOH) until the pH value is 6.5 to obtain a bloom-forming cyanobacteria acidolysis product;
2) the content of microcystins in the acidolysis product of the bloom-forming cyanobacteria is 0.06 microgram/kg according to the sanitary Standard for Drinking Water (GB5749-2006, which is described in the method, and is determined and analyzed by the high performance liquid chromatography technology;
3) in the acidolysis products of the bloom-forming cyanobacteria, the composition of free amino acids in the cyanobacteria glycolysis products is analyzed by adopting an amino acid automatic analyzer according to a method of 'determination of amino acids in feed' (GB/TB18246-2000), and the specific analysis result is as follows: the acidolysis degree conversion rate of the acidolysis product of the bloom-forming cyanobacteria reaches 91.5 percent, and the relative content of amino acid in the acidolysis product reaches 43.32 percent.
The preparation method of the acidolysis product of the bloom-forming cyanobacteria is the prior art, and can also be prepared by other methods as long as the acidolysis product of the bloom-forming cyanobacteria meets the requirement that the content of microcystin in dry substances is lower than 2.0 micrograms/kg.
Example 4
(1) The seashell culture pond water fertilizing paste prepared in the example 1 is sprayed according to the dosage of 1.2 kg/mu and the frequency of about 8 days/time when the transparency reaches 40 cm in a culture pond for intercropping 16 mu Ruditapes philippinarum-prawns of a sea water yield limited company in the new coastal area of Tianjin from 7 middle ten days to 8 middle ten days in 2019, the fertilizer water is sprayed for 4 times, the culture production test is carried out for 35 days, and other culture production operations are not changed; a16 mu clam culture pond which is not fertilized with water all the time is taken as a control group.
(2) Collecting a water sample: 1 qualitative sample and 1 quantitative sample are respectively collected at each sampling point arranged in 10 months and 30 days. Collection of qualitative samples: dragging slowly around the shape of infinity for 1-3 minutes on the surface layer of the water body to 50 cm by using a No. 25 plankton net, and fixing the collected sample by using 1.5% Rogowski fluid; and (3) collecting a quantitative sample, namely sampling by using a 1-liter organic glass water collector, fixing by using 1.5% Rogowski solution, concentrating into a 50-milliliter glass bottle after 48 hours, collecting another 1-liter water sample, directly performing vacuum filtration by using a 0.22-micrometer microporous membrane, and remaining filtrate for detecting the content of the microcystic toxins in the water.
(3) Collecting a water sample at 10 am 3 rd morning after each water fertilization, qualitatively and quantitatively monitoring phytoplankton in the pond and detecting the content of microcystin in the water, wherein the specific monitoring result shows that:
① 4 after water fertilization for 4 times, the phytoplankton identified in the clam culture pond is divided into 7 phyla 20 species, 8 phyla diatoms, 4 phyla chlorella, 2 phyla euglena, 3 phyla cyanobacteria, 1 species of cryptophyceae, chrysophyta and dinoflagellate, while the phytoplankton identified in the clam culture pond which is parallel to the phytoplankton identified all the time without water fertilization is divided into 4 phyla 13 species, 3 species of diatoms, 3 species of chlorophyta, 1 species of euglena, 3 species of cyanobacteria, 1 species of cryptophyceae, chrysophyta and dinoflagellate, so that the diversity of the phytoplankton in the clam culture pond can be greatly improved under the action of the invention.
② quantitative monitoring results of phytoplankton in the aquaculture pond, the results in the table are the average value of the 4 times of monitoring results, it can be seen that the total density of the phytoplankton in the clam aquaculture pond with rich water is improved by 3.35 times compared with the shellfish aquaculture pond without wastewater, specifically, the density of the main bait microalgae (green algae and diatom) of the clam after the water is rich is 3.68 times of that in the pond without rich water, and the water-fertilizing effect is very good.
No microcystin is detected in the water sample of the clam culture pond on the 3 rd day after ③ 4 times of water fertilization (the detection limit of an instrument is 5 ng/L).
(4) After the water fertilizing test of the 35-day clam culture pond, the growth conditions of the clams are as follows:
on the whole, the fresh weight and the shell length of the clam culture are obviously improved by fertilizing the water in the 35-day clam culture pond, and the seawater shellfish growth can be obviously improved by utilizing the fertilized water provided by the invention.
The above-mentioned embodiments are only for illustrating the technical ideas and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and to carry out the same, and the present invention shall not be limited to the embodiments, i.e. the equivalent changes or modifications made within the spirit of the present invention shall fall within the scope of the present invention.
Claims (4)
1. A water fertilizing paste for seawater shellfish pond culture is characterized in that microalgae acidolysis products, humic acid, shell powder, bacillus subtilis powder and feeding trace elements are mixed and stirred uniformly to prepare a paste according to the mass fraction of 50-70: 10-20: 4-6: 3-3.8: 0.1-0.2; the microalgae acidolysis product is a bloom-forming cyanobacteria acidolysis product, and the content of microcystin in dry matter is lower than 2.0 micrograms/kg.
2. The water fertilizing paste for seawater shellfish pond culture as claimed in claim 1, wherein the water bloom cyanobacteria acidolysis product is prepared by using concentrated hydrochloric acid to acidolyze water bloom cyanobacteria mud with water content of 70% -90%, wherein the volume of the concentrated hydrochloric acid is as follows: the volume of the cyanobacteria bloom mud is 15-30%.
3. The use method of the water fertilizing paste for the seawater shellfish pond culture as claimed in claim 1 or 2, characterized in that when the transparency of the seawater shellfish culture pond is higher than 40 cm, the paste is diluted according to the dosage of 1-1.5 kg/mu and then sprinkled for use.
4. The use method of the water fertilizing paste for the pond culture of seawater shellfish according to claim 3, characterized in that the seawater shellfish is clam, oyster and ruditapes philippinarum.
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| CN113396845A (en) * | 2021-07-23 | 2021-09-17 | 辽宁省海洋水产科学研究院 | Method for improving survival rate of two-year-old patinopecten yessoensis cultured in raft |
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Effective date of registration: 20221102 Address after: Agriculture and Animal Husbandry Circular Economy Industrial Park, Nalang Town, Zhuoni County, Gannan Tibetan Autonomous Prefecture, Gansu Province, 747600 Patentee after: Zhuoni County Jiufeng Ecological Pharmaceutical Co.,Ltd. Address before: 300384 No. 22, Jing Jing Road, Xiqing District, Tianjin Patentee before: TIANJIN AGRICULTURAL University |