CN113368019A - 一种铁皮石斛发酵液、铁皮石斛精华液及制备方法和用途 - Google Patents
一种铁皮石斛发酵液、铁皮石斛精华液及制备方法和用途 Download PDFInfo
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Abstract
本发明公开了一种铁皮石斛发酵液、铁皮石斛精华液及制备方法和用途,包括如下质量份的原料制备得到:石斛10‑30份,白藜芦醇10‑15份,小麦低聚肽0.2‑0.25份,碳酸氢钠0.3‑0.5份,维生素0.20‑0.25份,肠膜明串珠菌0.005‑0.008份,乳酸乳球菌0.005‑0.008份,纤维素酶0.3‑0.5份,果胶酶0.3‑0.5份,纯净水50‑80份。本发明的铁皮石斛发酵液、铁皮石斛精华液的制备方法和用途具有多糖转换率高等优点。
Description
技术领域
本发明涉及生物发酵技术领域,具体涉及一种铁皮石斛发酵液、铁皮石斛精华液及制备方法和用途。
背景技术
铁皮石斛被誉为中华九大仙草之首,素有药中黄金的美誉,主要功效为清润解毒、滋养肌肤、补五脏虚劳、内脏不足、养肝明目等功效,多用于药品和食品中,而铁皮石斛中含有大量胶质,而胶质是补充人体皮肤中胶原蛋白的主要来源,因此,将铁皮石斛的有效成分应用于养肤护肤中还有待开发和研究。
现有技术中,提取铁皮石斛中的有效物质效率较低,得到的铁皮石斛发酵液内的多糖转换率较低,使得铁皮石斛发酵液难以有效地应用在养肤护肤领域。
发明内容
本发明提供了一种铁皮石斛发酵液、铁皮石斛精华液及制备方法和用途,旨在解决上述问题。
根据本申请实施例提供的一种铁皮石斛发酵液、铁皮石斛精华液及制备方法和用途,所述铁皮石斛发酵液包括如下质量份的原料制备得到:石斛10-30份,白藜芦醇10-15份,小麦低聚肽0.2-0.25份,碳酸氢钠0.3-0.5份,维生素0.20-0.25份,肠膜明串珠菌0.005-0.008份,乳酸乳球菌0.005-0.008份,纤维素酶0.3-0.5份,果胶酶0.3-0.5份,纯净水50-80份。
为了解决上述技术问题,提供一种铁皮石斛发酵液的制备方法,包括如下步骤:
步骤S1:将24份石斛至60℃烘箱中烘干,烘干时长为12小时,烘干后置于高速粉碎机中,粉碎至粉末待用;将石斛粉末溶于55倍纯净水中,加入0.25份维生素C,高速均质20min,烘干,过80目筛,灭菌得到铁皮石斛细粉;
步骤S2:将铁皮石斛细粉转移至酶解罐中,加入0.45份纤维素酶、0.35份果胶酶和64.37份纯净水,升温至50℃,酶解90min,加入10份海藻提取物、0.25份小麦低聚肽,搅拌均匀,加入0.32份碳酸氢钠,调节pH值至6-7;
步骤S3:将酶解后的料体至于已消毒的发酵罐中,升温至90℃,保温30min;
步骤S4:降温至25℃,在已消毒的酶解料体中加入发酵菌,0.005份肠膜明串珠菌和0.005份乳酸乳球菌,发酵72小时;
步骤S5:将发酵好的料体用40目滤布进行过滤,得到铁皮石斛发酵液。
优选地,所述步骤S5之后还包括:
步骤S6:铁皮石斛投料量为w,将得到的铁皮石斛发酵液加入5倍无水乙醇,搅拌沉淀,静置5min后离心分离沉淀物,将沉淀物在65℃下烘干,得到铁皮石斛多糖w1,计算多糖转化率a=w1/w*100%。
为了解决上述技术问题,提供一种铁皮石斛精华液,包括如下质量份的原料制备得到:水52%,银耳提取物2%,聚谷氨酸1%,芽孢杆菌提取物5%,甘油10%以及铁皮石斛发酵液30%。
为了解决上述技术问题,提供一种铁皮石斛精华液的制备方法,包括如下步骤:
步骤S8:在容器中加入52%的水、2%的银耳提取物、1%的聚谷氨酸、5%的芽孢杆菌提取物、10%的甘油以及30%的铁皮石斛发酵液,混合均匀;
步骤S9:混合均匀后的液体进行121℃灭菌,待混合均匀后的液体降温至40℃后,进行无菌灌装处理。
优选地,所述铁皮石斛发酵液用于制备具有高多糖转化率的饮品、保健品或护肤品。
本申请实施例提供的技术方案可以包括以下有益效果:本申请设计了一种铁皮石斛发酵液、铁皮石斛精华液及制备方法和用途,通过石斛,白藜芦醇,小麦低聚肽,碳酸氢钠,维生素,肠膜明串珠菌,乳酸乳球菌,纤维素酶,果胶酶以及纯净水配置而成的铁皮石斛发酵液具有多糖转换率高的优点。采用铁皮石斛发酵液的制备方法,通过该方法获得的铁皮石斛发酵液具有多糖转换率高的优点,所述铁皮石斛精华液的稳定性好、气味淡,采用铁皮石斛精华液进行抗衰测试,患者的细纹减淡效果明显,所述铁皮石斛精华液为一款纯天然温和的具有抗衰作用的精华。
附图说明
为了更清楚地说明本发明实施例技术方案,下面将对实施例描述中所需要使用的附图作简单地介绍,显而易见地,下面描述中的附图是本发明的一些实施例,对于本领域普通技术人员来讲,在不付出创造性劳动的前提下,还可以根据这些附图获得其他的附图。
图1是本发明一种铁皮石斛发酵液的制备方法的流程示意图;
图2是本发明一种铁皮石斛精华液的制备方法的流程示意图;
图3是本发明一种铁皮石斛精华液的抗衰测试结果图;
图4是本发明铁皮石斛精华液的鸡胚绒毛尿囊膜试验结果图。
具体实施方式
下面将结合本发明实施例中的附图,对本发明实施例中的技术方案进行清楚、完整地描述,显然,所描述的实施例是本发明一部分实施例,而不是全部的实施例。基于本发明中的实施例,本领域普通技术人员在没有做出创造性劳动前提下所获得的所有其他实施例,都属于本发明保护的范围。
还应当理解,在此本发明说明书中所使用的术语仅仅是出于描述特定实施例的目的而并不意在限制本发明。如在本发明说明书和所附权利要求书中所使用的那样,除非上下文清楚地指明其它情况,否则单数形式的“一”、“一个”及“该”意在包括复数形式。
还应当进一步理解,在本发明说明书和所附权利要求书中使用的术语“和/或”是指相关联列出的项中的一个或多个的任何组合以及所有可能组合,并且包括这些组合。
本发明公开了一种铁皮石斛发酵液,包括如下质量份的原料制备得到:石斛10-30份,白藜芦醇10-15份,小麦低聚肽0.2-0.25份,碳酸氢钠0.3-0.5份,维生素0.20-0.25份,肠膜明串珠菌0.005-0.008份,乳酸乳球菌0.005-0.008份,纤维素酶0.3-0.5份,果胶酶0.3-0.5份,纯净水50-80份。
请参阅图1,本发明公开了一种铁皮石斛发酵液的制备方法10,包括如下步骤:1)磨粉;2)酶解;3)提取灭菌;4)发酵;5)过滤。为了进行铁皮石斛多糖转化率测试,进行以下实验:
实验组1:
1)磨粉,步骤S1:将24份新鲜石斛至60℃烘箱中烘干,烘干时长为12小时,烘干后置于高速粉碎机中,粉碎至粉末待用;将石斛粉末溶于55倍纯净水中,加入0.25份维生素C(防止石斛被氧化),高速均质20min,烘干,过80目筛,灭菌得到铁皮石斛细粉;
2)酶解,步骤S2:将铁皮石斛细粉转移至酶解罐中,加入0.45份纤维素酶、0.35份果胶酶和64.37份纯净水,升温至50℃,酶解90min,加入10份海藻提取物、0.25份小麦低聚肽,搅拌均匀,加入0.32份碳酸氢钠,调节pH值至6-7;
3)提取灭菌,步骤S3:将酶解后的料体至于已消毒的发酵罐中,升温至90℃,保温30min;
4)发酵,步骤S4:降温至25℃,在已消毒的酶解料体中加入发酵菌,0.005份肠膜明串珠菌和0.005份乳酸乳球菌,发酵72小时;
5)过滤,步骤S5:将发酵好的料体用40目滤布进行过滤,得到铁皮石斛发酵液。
对照组1:
1)磨粉:将24份新鲜石斛至60℃烘箱中烘干,烘干时长为12小时,烘干后置于高速粉碎机中,粉碎至粉末待用;
2)酶解:将铁皮石斛细粉转移至酶解罐中,加入0.45份纤维素酶、0.35份果胶酶和58.24份纯净水,升温至50℃,酶解90min,加入10份海藻提取物、0.25份小麦低聚肽,搅拌均匀,加入0.45份碳酸氢钠,调节pH值至6-7;
3)提取灭菌:将酶解后的料体至于已消毒的发酵罐中,升温至90℃,保温30min;
4)发酵:降温至25℃,在已消毒的酶解料体中加入发酵菌,0.005份肠膜明串珠菌和0.005份乳酸乳球菌,发酵72小时;
5)过滤:将发酵好的料体用40目滤布进行过滤,得到铁皮石斛发酵液。
对照组2:
1)磨粉:将24份新鲜石斛至60℃烘箱中烘干,烘干时长为12小时,烘干后置于高速粉碎机中,粉碎至粉末待用;将石斛粉末溶于55倍纯净水中,加入0.25份维生素C(防止石斛被氧化),高速均质20min,烘干,过80目筛,灭菌得到铁皮石斛细粉;
2)酶解:将铁皮石斛细粉转移至酶解罐中,加入0.45份纤维素酶、0.35份果胶酶和64.365份纯净水,升温至50℃,酶解90min,加入10份海藻提取物、0.25份小麦低聚肽,搅拌均匀,加入0.45份碳酸氢钠,调节pH值至6-7;
3)提取灭菌:将酶解后的料体至于已消毒的发酵罐中,升温至90℃,保温30min;
4)发酵:降温至25℃,在已消毒的酶解料体中加入发酵菌,0.0075份肠膜明串珠菌和0.0075份乳酸乳球菌,发酵72小时;
5)过滤:将发酵好的料体用40目滤布进行过滤,得到铁皮石斛发酵液。
对照组3:
1)磨粉:将24份新鲜石斛至60℃烘箱中烘干,烘干时长为12小时,烘干后置于高速粉碎机中,粉碎至粉末待用;将石斛粉末溶于55倍纯净水中,加入0.25份维生素C(防止石斛被氧化),高速均质20min,烘干,过80目筛,灭菌得到铁皮石斛细粉;
2)酶解:将铁皮石斛细粉转移至酶解罐中,加入0.45份纤维素酶、0.35份果胶酶和64.365份纯净水,升温至50℃,酶解90min,加入10份海藻提取物、0.25份小麦低聚肽,搅拌均匀,加入0.45份碳酸氢钠,调节pH值至6-7;
3)提取灭菌:将酶解后的料体至于已消毒的发酵罐中,升温至90℃,保温30min;
4)发酵:降温至25℃,在已消毒的酶解料体中加入发酵菌,0.0025份肠膜明串珠菌和0.0025份乳酸乳球菌,发酵72小时;
5)过滤:将发酵好的料体用40目滤布进行过滤,得到铁皮石斛发酵液。
对照组4:
1)磨粉:将24份新鲜石斛至60℃烘箱中烘干,烘干时长为12小时,烘干后置于高速粉碎机中,粉碎至粉末待用;将石斛粉末溶于55倍纯净水中,加入0.25份维生素C(防止石斛被氧化),高速均质20min,烘干,过80目筛,灭菌得到铁皮石斛细粉;
2)酶解:将铁皮石斛细粉转移至酶解罐中,加入0.45份纤维素酶、0.35份果胶酶和64.365份纯净水,升温至50℃,酶解90min,加入5份海藻提取物、0.25份小麦低聚肽,搅拌均匀,加入0.45份碳酸氢钠,调节pH值至6-7;
3)提取灭菌:将酶解后的料体至于已消毒的发酵罐中,升温至90℃,保温30min;
4)发酵:降温至25℃,在已消毒的酶解料体中加入发酵菌,0.005份肠膜明串珠菌和0.005份乳酸乳球菌,发酵72小时;
5)过滤:将发酵好的料体用40目滤布进行过滤,得到铁皮石斛发酵液。
综上,计算铁皮石斛的多糖转化率:
铁皮石斛的投料量为w,将实验得到的铁皮石斛发酵液加入5倍无水乙醇,搅拌沉淀,静置5min后离心分离沉淀物,将沉淀物在65℃下烘干,得到铁皮石斛多糖w1,多糖转化率a=w1/w*100%。得到数据如下表1所示。
铁皮石斛多糖得率% | |
实验组1 | 34.8 |
对照组1 | 22.8 |
对照组2 | 35.1 |
对照组3 | 21.3 |
对照组4 | 25.6 |
表1
实验结论:
1.实验组1与对照组1对比,对照组1多糖转化率明显降低,说明处理后的铁皮石斛粉末能够提升铁皮石斛多糖的转化率;
2.实验组1与对照组2或对照组3对比,提升发酵菌浓度能提升多糖转化率,但效果不明显,但是减低发酵菌浓度,多糖转化率明显降低,根据性价比和实际的多糖转化率,选择0.005份肠膜明串珠菌和0.005份乳酸乳球菌的性价比更高;
3.实验组1与对照组4对比,减低海藻提取物含量,会使多糖转换率,海藻提取物对铁皮石斛发酵有增益效果。
本发明公开了一种铁皮石斛精华液,利用铁皮石斛发酵液制备,包括如下质量份的原料制备得到:水52%,银耳提取物2%,聚谷氨酸1%,芽孢杆菌提取物5%,甘油10%以及铁皮石斛发酵液30%。
所述铁皮石斛精华液的制备如下表2:
表2
请参阅图2,所述铁皮石斛精华液的生产工艺如下,包括以下步骤:
步骤S8:在容器中按比例加入水、银耳提取物、聚谷氨酸、芽孢杆菌提取物、甘油以及铁皮石斛发酵液,并混合均匀;
步骤S9:将混合均匀后的液体进行121℃灭菌,待混合均匀后的液体降温至40℃后,进行无菌灌装处理。
铁皮石斛精华液制备的抗衰测试:取10组患者对6组样品进行眼周皱纹进行测试,测试周期为一个月,其中对照组1的测试者细纹无明显减少,实验组1的测试者细纹有轻微的减少,实验组2-5的测试者细纹有明显的减少,且铁皮石斛发酵液的浓度越高,患者的细纹减淡效果越明显,但是,铁皮石斛发酵液浓度与产品气味效果成负相关关系,在铁皮石斛发酵液浓度达到40%时,测试者不太能接受铁皮石斛发酵液带来的气味,另外,料体色泽也偏黄,因此,中药液铁皮石斛发酵液的最佳浓度应控制在30%含量,该浓度下的铁皮石斛精华液美容护肤效果最佳且无特殊气味、颜色也不会偏黄。实验组4的测试结果可参照图3。
安全性:对实验组4的料体鸡胚绒毛尿囊膜试验中,并无刺激性反应,该配方温和无刺激,适合敏感肌使用。
鸡胚绒毛尿囊膜试验(刺激性测试):
1.对照设置:0.9%氯化钠溶液为阴性对照/IS值=0.0;0.1mol/L氢氧化钠为阳性对照/IS值14.8.
2.样品检验:取实验组4样品0.3ml直接滴加与绒毛尿囊膜表面,观察绒毛尿囊膜反应情况,在5min内观察初学、凝血、浑浊和血管溶解的时间。
3.实验结论:无刺激性,实验结果如图4所示。
测试结果显示:铁皮石斛精华液为一款纯天然温和的具有抗衰作用的精华。
本发明的上述铁皮石斛发酵液具有十分显著的高多糖转化率,因此,本发明提供的所述铁皮石斛发酵液可用于制备具有高多糖转化率的饮品、保健品或护肤品。饮品、保健品或护肤品具有本领域常规的含义,且并不属于上下位概念。
本申请实施例提供的技术方案可以包括以下有益效果:本申请设计了一种铁皮石斛发酵液、铁皮石斛精华液及制备方法和用途,通过石斛,白藜芦醇,小麦低聚肽,碳酸氢钠,维生素,肠膜明串珠菌,乳酸乳球菌,纤维素酶,果胶酶以及纯净水配置而成的铁皮石斛发酵液具有多糖转换率高的优点。采用铁皮石斛发酵液的制备方法,通过该方法获得的铁皮石斛发酵液具有多糖转换率高的优点,所述铁皮石斛精华液的稳定性好、气味淡,采用铁皮石斛精华液进行抗衰测试,患者的细纹减淡效果明显,所述铁皮石斛精华液为一款纯天然温和的具有抗衰作用的精华。
以上所述,仅为本发明的具体实施方式,但本发明的保护范围并不局限于此,任何熟悉本技术领域的技术人员在本发明揭露的技术范围内,可轻易想到各种等效的修改或替换,这些修改或替换都应涵盖在本发明的保护范围之内。因此,本发明的保护范围应以权利要求的保护范围为准。
Claims (6)
1.一种铁皮石斛发酵液,其特征在于,包括如下质量份的原料制备得到:石斛10-30份,白藜芦醇10-15份,小麦低聚肽0.2-0.25份,碳酸氢钠0.3-0.5份,维生素0.20-0.25份,肠膜明串珠菌0.005-0.008份,乳酸乳球菌0.005-0.008份,纤维素酶0.3-0.5份,果胶酶0.3-0.5份,纯净水50-80份。
2.一种铁皮石斛发酵液的制备方法,其特征在于,包括如下步骤:
步骤S1:将24份石斛至60℃烘箱中烘干,烘干时长为12小时,烘干后置于高速粉碎机中,粉碎至粉末待用;将石斛粉末溶于55倍纯净水中,加入0.25份维生素C,高速均质20min,烘干,过80目筛,灭菌得到铁皮石斛细粉;
步骤S2:将铁皮石斛细粉转移至酶解罐中,加入0.45份纤维素酶、0.35份果胶酶和64.37份纯净水,升温至50℃,酶解90min,加入10份海藻提取物、0.25份小麦低聚肽,搅拌均匀,加入0.32份碳酸氢钠,调节pH值至6-7;
步骤S3:将酶解后的料体至于已消毒的发酵罐中,升温至90℃,保温30min;
步骤S4:降温至25℃,在已消毒的酶解料体中加入发酵菌,0.005份肠膜明串珠菌和0.005份乳酸乳球菌,发酵72小时;
步骤S5:将发酵好的料体用40目滤布进行过滤,得到铁皮石斛发酵液。
3.根据权利要求2所述铁皮石斛发酵液的制备方法,其特征在于,所述步骤S5之后还包括:
步骤S6:铁皮石斛投料量为w,将得到的铁皮石斛发酵液加入5倍无水乙醇,搅拌沉淀,静置5min后离心分离沉淀物,将沉淀物在65℃下烘干,得到铁皮石斛多糖w1,计算多糖转化率a=w1/w*100%。
4.一种铁皮石斛精华液,其特征在于,包括如下质量份的原料制备得到:水52%,银耳提取物2%,聚谷氨酸1%,芽孢杆菌提取物5%,甘油10%以及铁皮石斛发酵液30%。
5.一种铁皮石斛精华液的制备方法,其特征在于,包括如下步骤:
步骤S8:在容器中加入52%的水、2%的银耳提取物、1%的聚谷氨酸、5%的芽孢杆菌提取物、10%的甘油以及30%的铁皮石斛发酵液,混合均匀;
步骤S9:混合均匀后的液体进行121℃灭菌,待混合均匀后的液体降温至40℃后,进行无菌灌装处理。
6.根据权利要求1所述铁皮石斛发酵液,其特征在于:所述铁皮石斛发酵液用于制备具有高多糖转化率的饮品、保健品或护肤品。
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