CN113348951A - High-stem-cutting rejuvenation method for sea buckthorn - Google Patents

High-stem-cutting rejuvenation method for sea buckthorn Download PDF

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CN113348951A
CN113348951A CN202110833313.5A CN202110833313A CN113348951A CN 113348951 A CN113348951 A CN 113348951A CN 202110833313 A CN202110833313 A CN 202110833313A CN 113348951 A CN113348951 A CN 113348951A
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rejuvenation
trunk
sea buckthorn
cutting
wax
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胡建忠
赵东晓
殷丽强
梁月
王东健
张玮
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Shan He Lin Beijing Water And Soil Conservation Technique Co ltd
Seabuckthorn Development Management Center Of Ministry Of Water Resources (water And Soil Conservation Plant Development Management Center Of Ministry Of Water Resources)
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Shan He Lin Beijing Water And Soil Conservation Technique Co ltd
Seabuckthorn Development Management Center Of Ministry Of Water Resources (water And Soil Conservation Plant Development Management Center Of Ministry Of Water Resources)
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Priority to CN202110833313.5A priority Critical patent/CN113348951A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/30Grafting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N3/00Preservation of plants or parts thereof, e.g. inhibiting evaporation, improvement of the appearance of leaves or protection against physical influences such as UV radiation using chemical compositions; Grafting wax
    • A01N3/04Grafting-wax
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/06Coniferophyta [gymnosperms], e.g. cypress
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/26Meliaceae [Chinaberry or Mahogany family], e.g. mahogany, langsat or neem

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Abstract

The application relates to the field of sea buckthorn rejuvenation, and particularly discloses a high-stem-cutting rejuvenation method for sea buckthorns, which comprises the following steps: s1, performing trunk cutting treatment on the sea buckthorn trees before sprouting, recording the trunk cutting height as H, wherein the value of H is 100-110cm, and then reserving 3-5 lateral branches within (80-H) cm of the trunk, wherein the reserved length of the lateral branches is 20-30 cm; s2, sealing the stubble of the trunk and the side branches with connecting wax; s3, when the length of the sprout of the trunk is more than 50cm, thinning branches, removing 40-50% of lateral branches in a mode of thinning every other branch, and pinching the retained branches. The high-stem-cutting rejuvenation method for the sea buckthorn trees has the advantages that the rejuvenation time of the sea buckthorn trees is short, the volume of the crowns in the current year can be recovered to about 2/3 before rejuvenation, the volume of the crowns in the next year after rejuvenation can reach the level before rejuvenation, and the economic benefit is high.

Description

High-stem-cutting rejuvenation method for sea buckthorn
Technical Field
The application relates to the technical field of sea-buckthorn rejuvenation, in particular to a high-stem-cutting rejuvenation method for sea-buckthorn.
Background
The sea buckthorn belongs to deciduous small trees, has strong adaptability to climate and soil, is resistant to severe cold wind and sand, is resistant to drought and high temperature, is resistant to barren soil, has strong tillering property, can be developed and utilized by fruit branches, and is a very important tree species for ecological environment treatment and economic development and utilization in the three north area and the southwest area of China.
"premature senility" means that the plant senescence comes ahead or the senescence starting age is earlier than the natural senescence time, for example, in a good habitat area, the growth period of camellia oleifera can reach hundreds of years, and in a poor area, senescence starts only at about 20 a; when the amount of the fir wood is 5-15a, the aging symptoms such as reduction of growth amount, accumulation amount and the like are presented. In recent years, the large-area premature senility phenomenon appears in the main planting area of the sea buckthorn in China, which is mainly reflected in that the aging initial age of the forest is advanced, the biomass of the population is reduced, the clonal propagation capacity is reduced, the old sea buckthorn enters the aging period after 5a under the condition of poor land conditions, particularly drought and no nurturing, and begins to fade or even die after about 8 a.
In the prior art, the sea-buckthorn is mainly updated by a stumping rejuvenation technology, namely, branches above the ground of the sea-buckthorn are cut at the contact part (with the height of 0-10 cm) of a tree body and the ground, so that the absorption and utilization of nutrients by the branches and the trunks are promoted, the growth vigor of the branches and the trunks is enhanced, and the plant diseases and insect pests are reduced. However, in areas with less interference to people and livestock, the fruit bearing requirement can be recovered in 4 years generally, and the rejuvenation time is longer; in areas with large interference to people and livestock, after stumping, the trees are difficult to recover due to the adverse effects of treading, gnawing and the like, the growth speed is slow, and the sea buckthorn gradually disappears along with the increase of the age.
In view of the above-mentioned related technologies, the inventors consider that a rejuvenation and renewal method for shortening the rejuvenation and renewal time of old sea buckthorn is urgently needed.
Disclosure of Invention
In order to shorten the rejuvenation and updating time of old trees, the application provides a high-stem-cutting rejuvenation method for sea buckthorn.
In a first aspect, the application provides a high-stem-cutting rejuvenation method for sea buckthorn, which adopts the following technical scheme:
a high-stem-cutting rejuvenation method for sea buckthorn comprises the following steps:
s1, performing trunk cutting treatment on the sea buckthorn trees before sprouting, recording the trunk cutting height as H, wherein the value of H is 100-110cm, and then reserving 3-5 lateral branches within (80-H) cm of the trunk, wherein the reserved length of the lateral branches is 20-30 cm;
s2, sealing the stubble of the trunk and the side branches with connecting wax;
s3, when the length of the sprout of the trunk is more than 50cm, thinning branches, removing 40-50% of lateral branches in a mode of thinning every other branch, and pinching the retained branches.
By adopting the technical scheme, the trunks of the sea buckthorn trees are removed by more than 100cm, so that old branches can be removed, and nutrient flow, water absorption and the like are promoted through the mutual restriction relationship among different parts of plants, and the renewal and the rejuvenation of the trunks are facilitated; in addition, the dry stubble openings are sealed by wax grafting, the wax grafting is soft in texture and can be coated on the stubble openings to seal the stubble openings, so that water evaporation, nutrient transition loss, rainwater invasion and plant diseases and insect pests are prevented, the wax grafting cannot cause any damage to the stubble openings, then branch thinning is carried out when the length of a sprout is more than 50cm, insect-infected branches, dry dead branches, useless overgrowth branches, excessively dense cross branches and overlapped branches are removed, the remained new branches grow vigorously, the growth trend is weakened, the ventilation and light transmission conditions of a tree body are improved, the photosynthetic efficiency of the leaves is improved, nutrient accumulation is increased, the remained branches are subjected to tip pinching treatment, the growth advantage at the top ends is removed, the growth of lateral buds is promoted, the branch growth and radial growth are promoted, the excessively dense photosynthetic branches and the overgrowth are prevented, the photosynthetic leaf area is increased, a large amount of products are accumulated, the formation of flower buds in autumn is promoted, the fruiting rate is improved, and the rejuvenated sea buckthorn tree adopting the scheme, in the current rejuvenation year, the crown can be recovered to about 2/3 before rejuvenation, the fruiting branch group is basically cultured, the number of flower buds is moderate, the flower buds can blossom and fruit in the next spring, the rejuvenation time is short, and the economic benefit can be improved.
Preferably, when the trunks of the sea buckthorn trees are cut and rejuvenated and the sea buckthorn trees are aged again, the second trunk cutting treatment is carried out before the sea buckthorn trees sprout, the height of the second trunk cutting is marked as H1, the value of H1 is H < H1 ≤ H +20 cm, or H > H1 ≥ H-20 cm;
when the value of H1 is H < H1 ≦ (H +20) cm, 3-5 lateral branches remain between H and H1 of the trunk, when the value of H1 is H > H1 ≧ (H-20) cm, 3-5 lateral branches remain between (H1-20) to H1, the retention length of the lateral branches is 20-30cm, and then step S2 and step S3 are performed.
By adopting the technical scheme, when the rejuvenation seabuckthorn trees grow and age again, the rejuvenation is carried out for the second time on the basis of the first rejuvenation, the trunk cutting height during the second rejuvenation can be larger than the first trunk cutting height or smaller than the first trunk cutting height, the position of the second trunk cutting treatment misses the position of the first trunk cutting treatment, the loss of the trunks is reduced, the cutoffs gradually form horse eyes or completely heal along with the hyperplasia healing of cortex, after the main branches and all the side branches on the tree crowns are removed, the side branches are reserved at different positions in advance, hidden buds can be stimulated to grow new branches, the new branches with the culture future are reserved to replace the original old branches, and then new tree crowns are formed, and the purpose of recovering the tree vigor and updating the rejuvenation is achieved.
Preferably, when the tree body is aged again after the second trunk pruning and rejuvenation, the third trunk pruning treatment is carried out before the sea buckthorn tree body sprouts, the third trunk pruning height is recorded as H2, the value of H2 is H < H2 ≤ H +20 cm, or H > H2 ≥ H-20 cm;
when the value of H2 is H < H2 ≦ (H +20) cm, 3-5 lateral branches are reserved between H and H2 of the trunk, when the value of H2 is H > H2 ≧ (H-20), 3-5 lateral branches are reserved between (H2-20) to H2, the retention length of the lateral branches is 20-30cm, and then step S2 and step S3 are carried out; h2 is not equal to H1.
By adopting the technical scheme, the sea-buckthorn trees which are subjected to secondary rejuvenation and have the aging phenomenon again are subjected to third trunk cutting rejuvenation, the third trunk cutting rejuvenation height is larger than or smaller than the first trunk cutting height, and is not equal to the second trunk cutting height, so that the positions of the two previous trunk cutting are avoided, the damage to the tree body is reduced, the number of lateral branches is increased, and the rejuvenation speed is accelerated.
Preferably, in step S1, the operation time of the drying process is early spring, 5-10 days before the sea-buckthorn sprouts.
By adopting the technical scheme, the trunk is cut in early spring, the wound is prevented from being frozen, the wound healing time is shortened, the evaporation of surface water is reduced due to the fact that the forest land is covered by the crowns and the dead leaves of the sea buckthorn, and meanwhile, the crowns and the dead leaves of the dead branches also play a role in heat preservation and moisture preservation, so that seedlings growing on the trunks of the sea buckthorn grow vigorously after the trunk is cut in spring; after stumping in rainy and autumn, the surface of the ground is exposed, and moisture is easy to evaporate in a large amount, so that the growth of the germinated seedlings is poor, the number of the germinated seedlings is small, and the effect is poor.
Preferably, in the step S2, the dosage of the grafted wax is 15-20 g/strain.
Through adopting above-mentioned technical scheme, connect the thickness of wax suitable, can prevent that the wax membrane is thicker, and easy the dropout from influencing the water retention ability, can prevent again that the wax membrane is too thin, and waterproof, bacterinertness are not enough.
Preferably, the connecting wax comprises the following components in parts by weight: 1-2 parts of rosin, 0.5-0.8 part of olive oil and 0.5-1 part of yellow wax.
By adopting the technical scheme, the rosin, the olive oil and the yellow wax are used for preparing the connecting wax, when the connecting wax is used, the connecting wax is melted and then coated on the dry stubbles of the trunk and the side branches, and the dry stubbles of the trunk and the side branches can be coated with a layer of uniform and thin bright paraffin layer, so that the dry stubbles of the trunk and the side branches are sealed, the evaporation amount of water is reduced, the pollution of rainwater and the like can be prevented, the callus for growth of the dry stubbles is not influenced, and the dry stubbles of the trunk and the side branches can still normally germinate and grow.
Preferably, the wax-grafting also comprises 0.1 to 0.5 weight part of tackifier, and the tackifier comprises rosin pentaerythritol ester and N, N' -1, 2-ethanediylbisacetostearyl (carbon) amide in a mass ratio of 1:0.3 to 0.5.
By adopting the technical scheme, the rosin and the yellow wax are easy to fall off from the dry stubbles when contacting the dry stubbles due to insufficient adhesive force, therefore, the rosin pentaerythritol ester and the N, N '-1, 2-ethanediylbisacetostearyl amide are used as the tackifier, the rosin pentaerythritol ester can improve the cohesive strength of the wax, improve the bonding force, promote the compatibility of the rosin and the N, N' -1, 2-ethanediylbisacetostearyl amide, avoid the phenomenon that the rosin is melted, the rosin and the N, N' -1, 2-ethanediylbisactadecyl (carbon) amide are separated out by layering or crystallization, the N, N' -1, 2-ethanediylbisactadecylamide has the characteristics of high melting point and low viscosity in a molten system, and is good in fluidity in a molten state, good in wettability and high in cohesive strength, and the tensile shear strength of the wax-connecting material can be improved.
Preferably, the wax-connecting agent also comprises 0.5-1 part of bactericide.
By adopting the technical scheme, when the wax is not sealed tightly and rainwater or other bacteria enter the dry stubbles of the trunks and the lateral branches of the sea buckthorns, the dry stubbles are easy to rot, and the bactericide is added, so that the bacteria can be effectively killed, and the dry stubbles are prevented from being rotted.
Preferably, the preparation method of the bactericide comprises the following steps:
(1) dissolving 1-2 parts by weight of chitosan and 2-4 parts by weight of carboxymethyl chitosan in 10 parts by weight of lactic acid with the concentration of 2%, and uniformly stirring to obtain a chitosan solution;
(2) adding 0.4-1 part by weight of beta-cyclodextrin and 0.5-1 part by weight of sodium benzoate into the chitosan solution obtained in the step (1), and performing ultrasonic dispersion to obtain a coating solution;
(3) 0.4-0.8 part of chinaberry powder, 1-2 parts of pseudolarix powder, 0.3-0.6 part of beta-indoleacetic acid and 1-1.5 parts of hollow mesoporous silica microspheres are uniformly mixed to form sterilization particles, the coating solution is uniformly atomized on the sterilization particles, and freeze drying is carried out to prepare the bactericide, wherein the mass ratio of the coating solution to the sterilization particles is 0.3-0.5: 1.
By adopting the technical scheme, the chitosan and the carboxymethyl chitosan are dissolved by using lactic acid, so that the chitosan solution can obtain good solubility and optimal viscosity, the bonding force of the wax to the trunk and the side branches of the tree body is further improved, then the beta-cyclodextrin and the sodium benzoate are added, the beta-cyclodextrin has moisture-preserving and water-blocking properties, the sodium benzoate has an antibacterial effect, the three are ultrasonically blended, then the coating solution is solidified on the surface of the sterilization particles by using a freeze-drying technology, strong interactions such as hydrogen bonds, ionic bonds and the like exist among the components in the coating solution, excellent compatibility is shown, therefore, the coating solution has better mechanical property after being solidified into a film, the freeze-drying technology enables the coating solution to generate micropores after being solidified, so that the sterilization particles in the interior are conveniently released, and the chitosan and the carboxymethyl chitosan in the coating solution are degradable substances, ultrasonic treatment and freeze-drying make the degradation speed of coating film accelerate, along with the going on of sprouting, the coating film degrades gradually, inside bactericidal granule releases gradually, the hollow mesoporous silica in the bactericidal granule has great specific surface area and good mesoporous structure, can load more chinaberry powder, cortex pseudolaricis powder and beta-indoleacetic acid, under hollow mesoporous silica's carrying, the particulate matter that has antibacterial action releases from the microporous membrane, and along with the degradation of microporous membrane, the release amount increases gradually, bactericidal action strengthens.
Preferably, the wax joint is prepared by the following method: heating olive oil to 65-70 deg.C, adding Colophonium and Cera flava, mixing, heating to 100 deg.C and 110 deg.C, adding tackifier, stirring for 20-30min, adding antibacterial agent, mixing, and cooling to normal temperature to obtain the final product.
By adopting the technical scheme, after the olive oil is heated, the rosin and the yellow wax are melted, the melted rosin and the yellow wax are mixed with the tackifier and the antibacterial agent to prepare the receiving wax, and when the receiving wax is used, the receiving wax is heated again, so that the operation is simple and convenient.
In summary, the present application has the following beneficial effects:
1. because the trunk cutting is carried out on the sea buckthorn tree body, the trunk cutting height is 100-plus 110cm, then the lateral branches are reserved, the trunk and the lateral branches are sealed by the grafting wax, and then the branches are thinned and pinched, the trunk cutting height is large, the growth of the lateral branches can be effectively promoted, the branches are prevented from growing too densely and overgrowing, the formation of flower buds is promoted, the volume of the crown in the current rejuvenation year can be recovered to about 2/3 before rejuvenation, the volume of the original tree body in the next rejuvenation year can be reached, the fruiting amount reaches the full bearing period level, the rejuvenation updating time is obviously shortened compared with a flat stubble technology, and the economic income is improved.
2. In the method, the sea-buckthorn trees which are aged again after being subjected to high trunk pruning rejuvenation are rejuvenated for the second time by adopting the method, the sea-buckthorn trees which are aged after being subjected to the second rejuvenation are rejuvenated for the third time, and the trunk pruning heights of the second rejuvenation and the third rejuvenation are different, so that the trunk pruning height during the first rejuvenation is avoided, the damage to the trunk can be reduced, the lateral branches can be stored and remained maximally, the rejuvenation speed is increased, and the rejuvenation time is shortened.
3. In the application, rosin, olive oil and yellow wax are used for preparing the grafting wax, and the tackifier prepared from rosin pentaerythritol ester and N, N' -1, 2-ethanediylbisacetostearyl (carbo) amide is added into the grafting wax, so that the bonding force of the grafting wax with the trunk and the lateral branches can be effectively increased, the grafting wax is prevented from being separated before the trunk and the lateral branches sprout, and the trunk and the lateral branches are prevented from being dehydrated too much and rotten due to bacterial infection.
4. In this application, preferably to the grafting wax of being made by rosin, olive oil and beeswax in add by the antiseptic that chitosan, cavity mesoporous silica etc. made through freeze-drying, chitosan and carboxymethyl chitosan are after supersound and freeze-drying, its degradation rate is accelerated, the cladding is on the granule that disinfects after freeze-drying, cortex pseudolaricis powder and the chinaberry powder that have antibiotic effect are under cavity mesoporous silica's loading, release from the micropore of coating film, along with the degradation of coating film, the release amount of antibiotic granule increases, thereby antibiotic effect has been increaseed along with the extension of time, after preventing to connect wax and lose sealed effect along with the extension of time, antibiotic effect descends.
Drawings
FIG. 1 is a tree body diagram of the sea buckthorn of the present invention after being cut;
FIG. 2 is a tree body diagram of the current year after rejuvenation and updating of Hippophae rhamnoides of the present invention;
FIG. 3 is a diagram of a fruiting tree of the present invention after rejuvenation and regeneration of Hippophae rhamnoides in one year.
Detailed Description
Examples of preparation of fungicides
Preparation examples 1-7 the chitosan was selected from Dalian Henchang source pharmaceutical chemical Co., Ltd, model number A5688; the carboxymethyl chitosan is selected from Sianjia and biotechnology limited company, and the product number is JH-465; the beta-cyclodextrin is selected from Ningbo Huayuan Biotech Co., Ltd, with a product number of 65223; the sodium benzoate is selected from chemical Limited of Ji nan Lu Wen Hao, with a product number of 0021; the cortex Meliae powder is selected from radix Angelicae Dahuricae Biotechnology, Inc., with product number of QZSW-1855; the cortex pseudolaricis powder is selected from Sierra Riemer bioengineering GmbH, with a product number of REL 0381; the beta-indoleacetic acid is selected from Wuhanfuxin Yunqi technology Co., Ltd, with the product number of FXY-B037; the hollow mesoporous silica microsphere is selected from Jiangsu Xiancheng nanometer material antibiotic Co., Ltd, with model number of 7440-21-3 and average particle size of 200 nm.
Preparation example 1: (1) according to the proportion in the table 1, 1kg of chitosan and 2kg of carboxymethyl chitosan are dissolved in 10kg of lactic acid with the concentration of 2 percent, and stirred for 3 hours to obtain a chitosan solution;
(2) adding 0.4kg of beta-cyclodextrin and 0.5kg of sodium benzoate into the chitosan solution obtained in the step (1), and performing ultrasonic dispersion for 3 hours under the conditions of power of 120W and frequency of 20kHz to obtain a coating solution;
(3) 0.4kg of chinaberry powder, 1kg of pseudolarix powder, 0.3kg of beta-indoleacetic acid and 1kg of hollow mesoporous silica microspheres are uniformly mixed to form bactericidal particles, the coating solution is uniformly atomized on the bactericidal particles and is frozen and dried for 2 hours at the temperature of minus 40 ℃ to prepare the bactericide, the atomization pressure is 0.2MPa, and the mass ratio of the coating solution to the bactericidal particles is 0.3: 1.
TABLE 1 antimicrobial preparation examples 1-7 raw material amounts
Figure BDA0003176305500000061
Preparation examples 2 to 3: the difference from preparation example 1 is that the amount of the raw materials was as shown in Table 1.
Preparation example 4: the difference from the preparation example 1 is that the mass ratio of the coating solution to the sterilization particles is 0.5:1, the freeze-drying temperature is-30 ℃, and the time is 3 hours.
Preparation examples 5 to 7: the difference from preparation example 1 is that the amount of the raw materials was as shown in Table 1.
Preparation examples 1 to 13 of catching wax
Preparation examples 1-13 the olive oil was selected from Jiangxi Huabang natural perfume GmbH, model No. J07, the rosin was selected from Jintenglong GmbH, Shenzhen, model No. KE-604, the yellow wax was selected from Hebei Qing beeswax GmbH, model No. 62, the rosin pentaerythritol ester was selected from Guangzhou Songbao chemical GmbH, model No. W105, N' -1, 2-ethanediylbisacetostearyl amide was selected from Shanghai eosin Karl, CAS model No. 110-30-5.
Preparation example 1: according to the raw material ratio in Table 2, 0.5kg of olive oil is heated to 65 ℃, 1kg of rosin and 0.5kg of yellow wax are added, and the materials are uniformly mixed to obtain the grafted wax.
Preparation example 2: according to the raw material ratio in Table 2, 0.8kg of olive oil is heated to 70 ℃, 2kg of rosin and 1kg of yellow wax are added, and the materials are uniformly mixed to obtain the grafted wax.
Preparation example 3: according to the raw material proportion in the table 2, 0.5kg of olive oil is heated to 65 ℃, 1kg of rosin and 0.5kg of yellow wax are added, the mixture is uniformly mixed, the temperature is raised to 100 ℃, 0.1kg of tackifier is added, the mixture is stirred for 20min and cooled to room temperature, and the wax is prepared, wherein the tackifier comprises rosin pentaerythritol ester and N, N' -1, 2-ethanediylbisacta (carbonamide) in a mass ratio of 1: 0.3.
Preparation example 4: according to the raw material proportion in the table 2, 0.5kg of olive oil is heated to 65 ℃, 1kg of rosin and 0.5kg of yellow wax are added, the mixture is uniformly mixed, the temperature is raised to 110 ℃, 0.5kg of tackifier is added, the mixture is stirred for 20min and cooled to room temperature, and the wax is prepared, wherein the tackifier comprises rosin pentaerythritol ester and N, N' -1, 2-ethanediylbisacta (carbonamide) in a mass ratio of 1: 0.3.
Preparation example 5: the difference from preparation example 3 is that the tackifier is pentaerythritol rosin ester.
Preparation example 6: the difference from preparation 3 is that the adhesion promoter is N, N' -1, 2-ethanediylbisactadecylamide.
Preparation example 7: according to the raw material proportion in the table 2, 0.5kg of olive oil is heated to 65 ℃, 1kg of rosin and 0.5kg of yellow wax are added, the mixture is uniformly mixed, the temperature is raised to 100 ℃, 0.1kg of tackifier is added, the mixture is stirred for 20min, 0.5kg of antibacterial agent is added, the mixture is uniformly stirred and cooled to room temperature, and the wax is prepared, wherein the tackifier comprises pentaerythritol ester of rosin and N, N' -1, 2-ethanediylbisacetostearyl (carbonamide) in a mass ratio of 1:0.3, and the antibacterial agent is prepared from the preparation example 1 of the antibacterial agent.
TABLE 2 preparation of grafted wax examples 1-13 raw material amounts
Figure BDA0003176305500000071
Preparation examples 8 to 13: the difference from preparation example 6 is that the source of the antibacterial agent is different, and is specifically shown in table 2.
Examples
Example 1: a high-stem-cutting rejuvenation method for sea buckthorn comprises the following steps:
s1, selecting a sea buckthorn tree with an aging phenomenon, taking a sea buckthorn tree with an aging phenomenon generated at 8a as an example, cutting off stems before the sea buckthorn tree sprouts, recording the height of the cut stems as H, keeping 3 lateral branches within (80-100) cm of a trunk, keeping the length of the lateral branches as 30cm, carrying out the cutting off stem treatment in early spring, and starting 5 days before the sea buckthorn sprouts;
s2, sealing the stubble of the trunk and the side branches with a connecting wax, wherein the connecting wax is selected from preparation example 1 of the connecting wax, the connecting wax is melted at 90 ℃, and then the connecting wax is coated on the stubble of the trunk and the side branches, and the using amount of the connecting wax is 15 g/trunk;
s3, when the length of the sprout of the trunk is more than 50cm, thinning branches, removing 40% of lateral branches in a mode of thinning every other branch, pinching the kept branches, and then carrying out appropriate fertilizer and water management, growing season branch thinning management, pest control and winter pruning management.
Example 2: a high-stem-cutting rejuvenation method for sea buckthorn comprises the following steps:
s1, selecting a sea buckthorn tree with an aging phenomenon, taking a sea buckthorn tree with an aging phenomenon generated at 8a as an example, cutting off stems before the sea buckthorn tree sprouts, recording the height of the cut stems as H, keeping the numerical value of the H as 100cm, keeping 5 lateral branches within (80-100) cm of a trunk, keeping the length of the lateral branches as 20cm, carrying out the cutting off treatment in early spring, and starting 8 days before the sea buckthorn sprouts;
s2, sealing the stubble of the trunk and the side branches with a connecting wax, wherein the connecting wax is selected from preparation example 2 of the connecting wax, the connecting wax is melted at 100 ℃, and then the connecting wax is coated on the stubble of the trunk and the side branches, and the using amount of the connecting wax is 18 g/trunk;
s3, when the length of the sprout of the trunk is more than 50cm, thinning branches, removing 50% of lateral branches in a mode of thinning every other branch, pinching the remaining branches, and then carrying out appropriate fertilizer and water management, growing season branch thinning management, pest control and winter pruning management.
Example 3: a high-stem-cutting rejuvenation method for sea buckthorn comprises the following steps:
s1, selecting a sea buckthorn tree with an aging phenomenon, taking a sea buckthorn tree with an aging phenomenon generated by 8a as an example, cutting off stems before the sea buckthorn tree sprouts, recording the height of the cut-off stems as H, keeping 5 lateral branches within (80-110) cm of a trunk, keeping the length of the lateral branches as 25cm, carrying out the cutting off stem treatment in early spring, and starting 10 days before the sea buckthorn sprouts;
s2, sealing the stubble of the trunk and the side branches with a connecting wax, wherein the connecting wax is selected from preparation example 3 of the connecting wax, the connecting wax is melted at 110 ℃, and then the connecting wax is coated on the stubble of the trunk and the side branches, and the using amount of the connecting wax is 20 g/trunk;
s3, when the length of the sprout of the trunk is more than 50cm, thinning branches, removing 45% of lateral branches in a mode of thinning every other branch, pinching the remaining branches, and then carrying out appropriate fertilizer and water management, growing season branch thinning management, pest control and winter pruning management.
Examples 4 to 11: a method for rejuvenating sea buckthorn with high cut stem is different from that of example 1 in the source of the grafting wax, and is shown in Table 3.
TABLE 3 sources of grafted wax in example-11
Examples Preparation example of catching wax
Example 1 Preparation example 1
Example 2 Preparation example 2
Example 3 Preparation example 3
Example 4 Preparation example 4
Example 5 Preparation example 5
Example 6 Preparation example 6
Example 7 Preparation example 7
Example 8 Preparation example 8
Example 9 Preparation example 9
Example 10 Preparation example 10
Example 11 Preparation example 11
Example 12 Preparation example 12
Example 13 Preparation example 13
Example 14: a sea-buckthorn high-stem-cutting rejuvenation method is different from the method in the embodiment 1,
s1, after 8 years of rejuvenation of the sea-buckthorn, when the sea-buckthorn trees are aged again, carrying out secondary trunk cutting treatment before the sea-buckthorn trees sprout, wherein the height of the secondary trunk cutting is H1, the value of H1 is 120cm, 3 lateral branches are reserved within 100 and 120cm of the trunk, and the reserved length of the lateral branches is 20 cm;
s2, sealing the stubble of the trunk and the side branches with a connecting wax, wherein the connecting wax is selected from the preparation example 1 of the connecting wax;
s3, when the length of the sprout of the trunk is more than 50cm, thinning branches, removing 40% of lateral branches in a mode of thinning every other branch, pinching the kept branches, and then carrying out appropriate fertilizer and water management, growing season branch thinning management, pest control and winter pruning management.
Example 15: a sea-buckthorn high trunk-cutting rejuvenation method is different from the embodiment 12 in that the value of the second trunk-cutting height H1 in the step S1 is 130cm, 3 lateral branches are reserved within 100-130cm of a trunk, and the reserved length of the lateral branches is 20 cm.
Example 16: a method for rejuvenating high trunks of sea buckthorns, which is different from example 12 in that the value of the second trunk height H1 in step S1 is 80cm, 3 side branches are retained within 60-80cm of the trunk, and the retained length of the side branches is 20 cm.
Example 17: a method for rejuvenating high trunks of sea buckthorns, which is different from example 12 in that the value of the second trunk height H1 in step S1 is 90cm, 3 side branches are retained within 70-90cm of the trunk, and the retained length of the side branches is 20 cm.
Example 18: a sea-buckthorn high trunk-cutting rejuvenation method is different from the method in example 14 in that when a tree body is aged again after being subjected to second trunk-cutting rejuvenation for 8 years, third trunk-cutting treatment is carried out before sea-buckthorn sprouts, the height of the third trunk-cutting treatment is H2, the value of H2 is 80cm, 3 lateral branches are reserved within 60-80cm of the trunk, and the reserved length of the lateral branches is 20 cm.
Example 19: a seabuckthorn high stem pruning rejuvenation method, which is different from the embodiment 15 in that when a tree body is aged again after being subjected to the second stem pruning rejuvenation for 8 years, the third stem pruning treatment is carried out before seabuckthorn sprouts, the height of the third stem pruning is H2, the value of H2 is 90cm, 3 lateral branches are reserved within 70-90cm of the trunk, and the reserved length of the lateral branches is 20 cm.
Example 20: a seabuckthorn high stem cutting rejuvenation method, which is different from the embodiment 16 in that when a tree body is aged again after being subjected to the second stem cutting rejuvenation for 8 years, the third stem cutting treatment is carried out before the seabuckthorn sprouts, the height of the third stem cutting is H2, the value of H2 is 120cm, 3 lateral branches are reserved within 100-120cm of the trunk, and the reserved length of the lateral branches is 20 cm.
Example 21: a seabuckthorn high stem cutting rejuvenation method, which is different from the embodiment 17 in that when a tree body is aged again after being subjected to the second stem cutting rejuvenation for 8 years, the third stem cutting treatment is carried out before the seabuckthorn sprouts, the height of the third stem cutting is H2, the value of H2 is 130cm, 3 lateral branches are reserved within 100-130cm of the trunk, and the reserved length of the lateral branches is 20 cm.
Comparative example
Comparative example 1: the high-stem-cutting rejuvenation method for sea buckthorn is different from the embodiment 1 in that a stumping rejuvenation method is adopted, and the stumping rejuvenation height is 8 cm.
Comparative example 2: a method for rejuvenating sea buckthorn with high stem cutting, which is different from the method in example 1 in that the stem cutting height in step S1 is 20 cm.
Comparative example 3: a method for rejuvenating sea buckthorn with high stem cutting, which is different from the method of example 1 in that the stem cutting height in step S1 is 50 cm.
Comparative example 4: a method for rejuvenating sea buckthorn with high stem cutting, which is different from the method in example 1 in that the stem cutting height in step S1 is 150 cm.
Comparative example 5: a method for rejuvenating sea buckthorn with high stem cutting, which is different from the method in example 1 in that the stem cutting height in step S1 is 200 cm.
Comparative example 6: a method for rejuvenating sea buckthorn with high cut-off, which is different from example 1 in that the cut-off process is performed in autumn in step S1.
Performance test
Kinematic viscosity detection of first, second wax
1. The graft wax was prepared in accordance with the methods described in preparation examples 1 to 13, and then the tensile shear strength of the graft wax was measured in accordance with GB/T7124-2008 "measurement of tensile shear strength of adhesive", and then the kinematic viscosity at 100 ℃ of the graft wax was measured in accordance with GB/T2794-1995 "measurement Standard of adhesive viscosity", and the measurement results are shown in Table 4.
2. Selecting a great-golden hawthorn tree which is three years old and has strong growth, developed root system, no plant diseases and insect pests and affinity with the scion as a stock, selecting a branch with the thickness close to that of the scion on the stock from a trunk part 3-7 days before grafting, trimming and flattening the cut port part of the branch, cutting a cut with the shape close to that of the end part of the scion on the stock by using a cutting and grafting knife according to the thickness of the scion, selecting a branch of a two-year-old sweet red hawthorn tree which is sunny, short in internode and full in development as the scion, inserting the cut scion into the cut of the stock inwards, aligning cambiums at two sides, and exposing the upper end of the cut surface of the scion by 0.1-0.5cm so as to be beneficial to healing growth of the stock and the scion; after grafting, the connector is bound by a plastic tape with the width of 1cm, grafting wax is coated on the connector, the falling-off condition of the grafting wax before emerging of a new bud is observed, 50 scions are correspondingly tested in each preparation example, and the data are counted in a table 4.
TABLE 4 Performance testing of the grafted waxes
Figure BDA0003176305500000101
As can be seen from the data in Table 4, the graft wax prepared using rosin, yellow wax and olive oil in preparation examples 1-2 had a kinematic viscosity of 13-139mm2S and a tensile shear strength of 22 to 24kg/cm26-8 scions fall off before sprouting, the adhesion force of the grafting wax and the dry stubbles is low, the scions are easy to fall off from the dry stubbles, and the residual grafting wax completely falls off from the sprouts after sprouting, which shows that the adhesion force of the grafting wax prepared in the preparation example 1-2 is not too tight, so that the sprouting is difficult to eject.
In the preparation examples 3-4, based on the preparation example 1, a tackifier consisting of rosin pentaerythritol ester and N.N' -1, 2-ethanediylbisactadecylamide is added, and as can be seen from the data in Table 4, the kinematic viscosity and the tensile shear strength of the grafting wax are increased, the falling rate of the grafting wax before sprouting is 0-0.2%, which is obviously lower than those in the preparation examples 1-2, and the grafting wax after sprouting completely falls off, which indicates that the grafting wax prepared in the preparation examples 3-4 has tight adhesion and falling prevention when dry stubbles are adhered, has no tight adhesion when sprouting and does not influence the sprouting of young buds.
In production examples 5 and 6, N.N' -1, 2-ethanediylbisacetostearyl (carbo) amide and pentaerythritol rosin were not added, respectively, and in production examples 5 to 6, the kinematic viscosity and tensile shear strength of the grafted wax were reduced and the amount of wax layer falling before the sprouts were not germinated was increased, as compared with production examples 3 to 4.
The number of the drops before the germination was 0 after the coating of the graft wax in preparation examples 7 to 13, and the graft wax was all dropped after the germination, and the adhesion of the graft wax in examples 7 to 13 was more excellent but did not affect the emergence of the germination.
Second, detection of rejuvenation effect of sea buckthorn
1. Experimental nature profile: selecting Temple town in the county of Kogyang of Shangliaoning, locating in western Liaoning, having topography belonging to hilly area of low mountain area, geographic position taste of 119 deg.C-52 deg.C-120 deg.C, average annual temperature of 8.7 deg.C, average annual rainfall of 475mm, accumulated temperature of 3589.1 deg.C, frost-free period of 159d, annual sunshine time of 2820.4h, and radiationTotal injection quantity 5878kJ/cm2The coating belongs to semi-arid continental climate, the coating is brown soil, the soil texture is mature light loam, and the thickness of the soil layer is 35-45 cm.
2. The test method comprises the following steps: under the condition of the same field, 19 mu of seabuckthorn fruit forest with the age of 8a and the same growth vigor is adopted and divided into 19 groups on average, 220 seabuckthorn trees are planted in each mu of seabuckthorn fruit forest, the tree height, the crown width and the seabuckthorn yield of the seabuckthorn trees are measured, the 19 groups of seabuckthorn trees are rejuvenated and updated according to the methods in examples 1-13 and comparative examples 1-6 respectively, the seabuckthorn tree bodies after stem cutting are shown in figure 1, the tree height, the crown width and the seabuckthorn yield of the seabuckthorn trees are detected in the current year of rejuvenation and the next year after rejuvenation, the tree height and the crown width are recorded in table 5 according to the average value of the 220 seabuckthorn trees, the tree bodies in the current year after rejuvenation are shown in figure 2, the fruit trees in the next year after rejuvenation are shown in figure 3, and the seabuckthorn yield is recorded in per mu yield; and simultaneously recording the average number of new branch sprouts, the average diameter of new branches and the average length of new branches all the year around before the seabuckthorn sprouts are not thinned in rejuvenation and updating, and recording the data in the table 6.
TABLE 5 rejuvenation effects of the high cut-stem rejuvenation method of Hippophae rhamnoides in examples 1-13 and comparative examples 1-6
Figure BDA0003176305500000111
Figure BDA0003176305500000121
As can be seen from the data in table 5, when 8a seabuckthorn trees aged in the ages of 8a are rejuvenated by the methods in examples 1 to 4 and examples 7 to 10, the tree height reaches about 2/3 of the original tree body in the current rejuvenation year, the crown volume reaches more than 60% of the crown volume before rejuvenation, and in the first rejuvenation year, the crown volume reaches the original crown volume and the fruit yield reaches the level before rejuvenation, compared with the ground stumping technology in comparative example 1, the rejuvenation method in the present application has strong tree body life and high ecological benefit, and has effective fruits for seabuckthorn trees aged in different ages, when 5a seabuckthorn trees are aged, the rejuvenation method in example 1 in the present application has the crown volume reaching 2/3 of the original crown in the current rejuvenation year, and the crown width reaches the original crown volume in the second rejuvenation year, compared with the conventional stubble cutting method, the rejuvenation time is shortened, and the economic benefit is improved.
Compared with the examples 3-4, the yield per mu of the seabuckthorn fruit forest in the same year of rejuvenation is reduced, the tree height is reduced, and the yield per mu of the seabuckthorn in one year after the rejuvenation is still lower than that in the examples 3-4; examples 11 to 13 compared with examples 7 to 10, the tree height and the yield per mu of seabuckthorn in the same year of rejuvenation were inferior to those of examples 7 to 10, and the yield per mu in one year after rejuvenation was inferior to those of examples 7 to 10.
In comparative example 1, the stumping rejuvenation method was used, and the stump height was 8cm, in the current year of stumping rejuvenation, the crown did not reach 55% before rejuvenation, and the height of the tree in the current year of rejuvenation was only 38% before rejuvenation, in the second year of rejuvenation, the crown volume was only within 70% before rejuvenation, and the height of the tree was 67% before rejuvenation, and the rejuvenation effect was not as good as in the present application, and it was necessary to continue to grow to the third or fourth year layer until the level before rejuvenation was completely reached.
Comparative examples 2 to 5 are different from example 1 in that the height of the trunk cut of comparative example 2 is 20cm, the height of the trunk cut of comparative example 3 is 50cm, the height of the trunk cut of comparative example 4 is 150cm, the height of the trunk cut of comparative example 5 is 200cm, the hippophae rhamnoides tree of comparative examples 2 to 3 is slow, the tree height is only 50% before rejuvenation in the current rejuvenation year, the tree height is only 83% before rejuvenation in the first year after rejuvenation, and the effect of example 1 is not achieved; in contrast, the growth rate of the hippophae rhamnoides trees in comparative examples 4 to 5 was slow, and the yield per mu was low.
Comparative example 6 compared with example 1, when the sea buckthorn was cut in autumn, the crown of the rejuvenated sea buckthorn tree in comparative example 4 was 48% higher in the current year of rejuvenation than in 84% higher in the first year of rejuvenation, which did not achieve the effect of the present application.
TABLE 6 Effect of the high stem-cut rejuvenation method in examples 1-13 and comparative examples 1-6 on shoot growth
Figure BDA0003176305500000131
As can be seen from the data in Table 6, the wax grafting prepared in the preparation example 1-2 of the wax grafting in the example 1-2 seals the cut dry stubbles, and after sealing, the number of new branches of seabuckthorn in the current year is 23.3-23.8, the diameter of the branch is 1.14-1.16cm, and the length of the branch is 57.6-58.3cm, so that the wax grafting has a good sealing effect and prevents water loss.
In the example 3-4, the grafting wax prepared in the preparation example 3-4 is adopted, compared with the example 1, the tackifier is added into the grafting wax, and compared with the example 1, the grafting wax prepared in the preparation example 3-4 is used for sealing the cut dry stubbles, the sprouting amount of new branches is increased, the diameter and the length of the sprout are increased, and the growth vigor of the branches is better.
In the case of the grafted wax prepared in examples 5-6, compared with example 1, the N, N' -1, 2-ethanediylbisacetostearyl (carbo) amide and the rosin pentaerythritol ester are not added into the tackifier, and the seabuckthorn trees renewed by the rejuvenation method in examples 5 and 6 have a significantly reduced new shoot number compared with example 3, and the diameter and length of the sprout are reduced, so that the growth vigor of the sprout is reduced.
In examples 7 to 10, the graft wax prepared in examples 7 to 10 was used, in which not only the tackifier but also the antibacterial agent prepared in antibacterial agent preparation examples 1 to 4 were respectively selected, and the rejuvenated hippophae rhamnoides trees obtained in examples 7 to 10 had increased number of new shoots, and had thicker sprouts, increased lengths and better growth vigor than those obtained in examples 1 to 2 and examples 3 to 4.
In examples 11 to 13, the graft waxes prepared in preparation examples 11 to 13 were used, respectively, wherein the antibacterial agent was not added with cortex pseudolaricis, chinaberry powder, and hollow mesoporous silica, respectively, and the rejuvenated hippophae rhamnoides in examples 11 to 13 had a decreased number of new shoot sprouts as compared with examples 7 to 10, and had thinned and shortened branches and poor growth of new branches.
In comparative example 1, the stumping rejuvenation method was used, the stumping height was 8cm, the number of new shoots germinated after rejuvenation was small, and the length and diameter of the shoots were not as long as those in example 1 of the present application.
Comparative examples 2 to 3 compared with example 1, comparative example 2 had a cut-off height of 20cm, comparative example 3 had a cut-off height of 50cm, and comparative examples 2 and 3 had a small number of sprouts and thinner shoots.
The height of the cut-off of the comparative example 4 is 150cm, the height of the cut-off of the comparative example 5 is 200cm, and after rejuvenation, the branches of the comparative example 4 and the comparative example 5 are thin and short, and the growth vigor is inferior to that of the example 1, although the number of the branches is large.
Compared with the example 1, the comparative example 6 has the advantages that the trunks of the sea buckthorn are cut in autumn, and after rejuvenation, the new branches of the sea buckthorn are few in germination quantity, thin in branches and poor in growth vigor.
Third, secondary rejuvenation effect detection of sea buckthorn
1. Experimental nature profile: selecting Temple town in the county of Kogyang of Shangliaoning, locating in western Liaoning, having topography belonging to hilly area of low mountain area, geographic position taste of 119 deg.C-52 deg.C-120 deg.C, average annual temperature of 8.7 deg.C, average annual rainfall of 475mm, accumulated temperature of > 10 deg.C of 3589.1 deg.C, frost-free period of 159d, annual sunshine time of 2820.4h, total radiation of 5878kJ/cm2The coating belongs to semi-arid continental climate, the coating is brown soil, the soil texture is mature light loam, and the thickness of the soil layer is 35-45 cm.
2. The test method comprises the following steps: taking and dividing a sea buckthorn fruit forest which is 8a old and has the same growth vigor into 4 mu groups on average under the same field condition, planting 220 sea buckthorn trees in each mu of sea buckthorn fruit forest, carrying out first rejuvenation according to the method in the embodiment 1, carrying out normal growth for 8 years after the first rejuvenation, when the sea buckthorn trees are aged again, carrying out second rejuvenation on the 4 groups of sea buckthorn trees by respectively adopting the methods in the embodiments 14-17, detecting the tree height, the crown breadth and the yield per mu of the sea buckthorn trees before the second rejuvenation, detecting the tree height, the crown breadth and the yield per mu of the sea buckthorn trees again in the current year and the first year after the rejuvenation, and recording the detection results in a table 7.
TABLE 7 rejuvenation effects of the methods in examples 14-17
Figure BDA0003176305500000141
As can be seen from the data in table 7, after the seabuckthorn trees are rejuvenated for the first time by the method in example 1 and grow for 8 years, when the aging phenomenon occurs, after the seabuckthorn trees are rejuvenated for the second time by the methods in examples 14 to 17, in the current rejuvenation year, the tree height can reach more than 60% of the tree height before the rejuvenation, and the volume of the crown can reach 3/2 of the volume of the original crown, and in the next rejuvenation year, the crown and the tree height of the seabuckthorn can reach the volume of the original crown and the original tree height, the rejuvenation of the seabuckthorn trees can be completed in one year, the stumping rejuvenation is performed, the rejuvenation time is obviously shortened, and the yield per mu can reach the full bearing stage level before the rejuvenation in the next year after the rejuvenation.
Fourth, detecting the third rejuvenation effect of sea buckthorn
1. Experimental nature profile: selecting Temple town in the county of Kogyang of Shangliaoning, locating in western Liaoning, having topography belonging to hilly area of low mountain area, geographic position taste of 119 deg.C-52 deg.C-120 deg.C, average annual temperature of 8.7 deg.C, average annual rainfall of 475mm, accumulated temperature of > 10 deg.C of 3589.1 deg.C, frost-free period of 159d, annual sunshine time of 2820.4h, total radiation of 5878kJ/cm2The coating belongs to semi-arid continental climate, the coating is brown soil, the soil texture is mature light loam, and the thickness of the soil layer is 35-45 cm.
2. The test method comprises the following steps: taking 4 acres of seabuckthorn fruit trees which are 8a old and have the same growth vigor under the same field condition, averagely dividing the seabuckthorn fruit trees into 4 groups, planting 220 seabuckthorn trees in each acre of seabuckthorn fruit trees, carrying out first rejuvenation the 4 groups of seabuckthorn trees according to the method in the embodiment 1, carrying out second rejuvenation on the 4 groups of seabuckthorn fruit trees according to the methods in the embodiments 14-17 after the rejuvenation is finished and normally growing for 8 years, and carrying out third rejuvenation by adopting the methods in the embodiments 18-21 when the seabuckthorn trees are aged again, namely carrying out second rejuvenation by adopting the method in the embodiment 14, carrying out third rejuvenation by adopting the method in the embodiment 18, carrying out second rejuvenation by adopting the method in the embodiment 15, carrying out third rejuvenation by adopting the method in the embodiment 19 and the like; the tree height, the crown width and the yield per mu of the seabuckthorn trees are detected before the third rejuvenation, the tree height, the crown width and the yield per mu of the seabuckthorn trees are detected again in the current year of the rejuvenation and the first year after the rejuvenation, and the detection results are recorded in the table 8.
TABLE 8 rejuvenation effects of the methods in examples 18-21
Figure BDA0003176305500000151
As can be seen from the data in table 8, the method in example 1 is used to perform a first rejuvenation on of a 8-year old seabuckthorn tree with aging, the method in examples 14 to 17 is used to perform a second rejuvenation when the seabuckthorn tree is aged again eight years after eight years of growth, and then the seabuckthorn tree is grown normally for eight years, the method in examples 18 to 21 is correspondingly used to perform a third rejuvenation when the seabuckthorn tree is aged, the trunk height of the seabuckthorn in the third rejuvenation is higher than 68% of the trunk height before the rejuvenation in the same year of the rejuvenation and the acre yield reaches about 65% of the trunk height before the rejuvenation, and the height of the seabuckthorn trunk in the last year after the rejuvenation is close to that before the rejuvenation and the crown width reaches the original volume, so that the yield reaches the original yield.
Adaptability detection of five-high stem-cutting rejuvenation method
1. Experimental nature profile: selecting Temple town in the county of Kogyang of Shangliaoning, locating in western Liaoning, having topography belonging to hilly area of low mountain area, geographic position taste of 119 deg.C-52 deg.C-120 deg.C, average annual temperature of 8.7 deg.C, average annual rainfall of 475mm, accumulated temperature of > 10 deg.C of 3589.1 deg.C, frost-free period of 159d, annual sunshine time of 2820.4h, total radiation of 5878kJ/cm2The coating belongs to semi-arid continental climate, the coating is brown soil, the soil texture is mature light loam, and the thickness of the soil layer is 35-45 cm.
2. The test method comprises the following steps: taking a sea buckthorn fruit forest which is 5a old and has the same growth vigor and is 4 mu, dividing the sea buckthorn fruit forest into 4 groups on average under the same field condition, wherein the sea buckthorn fruit forest is respectively marked as a first field, a second field, a third field and a fourth field, 220 sea buckthorn trees are planted in each mu of the sea buckthorn fruit forest, the 4 groups of sea buckthorn trees are rejuvenated for the first time according to the method in the embodiment 1 and grow normally for 5 years, when the senescence phenomenon occurs again, the sea buckthorn trees are rejuvenated for the second time by adopting the method in the embodiment 14 in one to four fields correspondingly by adopting the method in the embodiment 14 in one to four fields, and when the senescence phenomenon occurs again after the normal growth for 5 years, the sea buckthorn trees are rejuvenated for the second time by adopting the method in the embodiment 18 to four fields correspondingly by adopting the method in the embodiment 19 in one to three times, by analogy, the tree height, the crown width and the yield per mu of the 5a raw hippophae rhamnoides are detected before rejuvenation, the tree height, the crown width and the yield per mu are detected in the current year of the first rejuvenation and the one year after the rejuvenation, then the tree height, the crown width and the yield per mu are detected in the current year of the second rejuvenation and the three-time rejuvenation and the one year after the rejuvenation, the detection result of the hippophae rhamnoides after the first rejuvenation is recorded in table 9, the detection result after the second rejuvenation is recorded in table 10, and the detection result after the third rejuvenation is recorded in table 11.
TABLE 95 rejuvenation effects of raw Hippophae rhamnoides
Figure BDA0003176305500000161
TABLE 105 Secondary rejuvenation effects of raw Hippophae rhamnoides
Figure BDA0003176305500000162
TABLE 115A after three rejuvenations of raw Hippophae rhamnoides
Figure BDA0003176305500000171
As can be seen from the data in Table 9, even if the age of 5a of the young trees is old, when the aging phenomenon occurs, the trunk and the crown width of the young trees can reach more than 60% of the original trees by adopting the method in the application for rejuvenation and updating, the height of the trunks and the crown width of the young trees are equivalent to those of the young trees in the same year after rejuvenation, the rejuvenation of the sea buckthorn is completed only in one year, and the economic benefit is improved.
As can be seen from the data in table 10, after rejuvenating the 5 a-old and aged hippophae rhamnoides trees by the method of the present application, after five years of normal growth, when the aging occurs again, the hippophae rhamnoides subjected to one rejuvenation is rejuvenated by the method of the present application for two times, the height and crown width of the hippophae rhamnoides trees can reach about 65% of those before rejuvenation in the same year, and the height and volume of the original trees can reach those in the same year after rejuvenation.
As can be seen from the data in table 11, after rejuvenating 5a old sea buckthorn trees by the method of the present application, after growing for 5 years, the aging phenomenon occurs again, after rejuvenating for 5 years, after growing for 5 years, the aging phenomenon occurs, when rejuvenating three times by the method of the present application, the rejuvenation effect is good, in the same year, the volume and height of the crown of the tree can reach about 65% of those before rejuvenation, and in one year after rejuvenation, the yield per mu reaches the level before rejuvenation, and the rejuvenation can be completed only in one year, so that the rejuvenation time is shortened, the economic benefit is improved, and the rejuvenation effect is provided for aged sea buckthorn trees of different ages, and the rejuvenation effect is also provided for aged sea buckthorn leaves of different ages after rejuvenation, and the rejuvenation of sea buckthorn trees can be repeated.
The present embodiment is only for explaining the present application, and it is not limited to the present application, and those skilled in the art can make modifications of the present embodiment without inventive contribution as needed after reading the present specification, but all of them are protected by patent law within the scope of the claims of the present application.

Claims (10)

1. A high-stem-cutting rejuvenation method for sea buckthorn is characterized by comprising the following steps:
s1, performing trunk cutting treatment on the sea buckthorn trees before sprouting, recording the trunk cutting height as H, wherein the value of H is 100-110cm, and then reserving 3-5 lateral branches within (80-H) cm of the trunk, wherein the reserved length of the lateral branches is 20-30 cm;
s2, sealing the stubble of the trunk and the side branches with connecting wax;
s3, when the length of the sprout of the trunk is more than 50cm, thinning branches, removing 40-50% of lateral branches in a mode of thinning every other branch, and pinching the retained branches.
2. The high stem-cutting rejuvenation method for sea buckthorn according to claim 1, characterized in that: when the trunks of the sea buckthorn trees are cut and rejuvenated and the sea buckthorn trees are aged again, carrying out secondary trunk cutting treatment before the sea buckthorn trees sprout, wherein the height of the secondary trunk cutting is marked as H1, and the value of H1 is H which is more than H1 and less than or equal to (H +20) cm or H which is more than H1 and more than or equal to (H-20) cm;
when the value of H1 is H < H1 ≦ (H +20) cm, 3-5 lateral branches remain between H and H1 of the trunk, when the value of H1 is H > H1 ≧ (H-20) cm, 3-5 lateral branches remain between (H1-20) to H1, the retention length of the lateral branches is 20-30cm, and then step S2 and step S3 are performed.
3. The high stem-cutting rejuvenation method for sea buckthorn according to claim 2, characterized in that: when the tree body is aged again after the second trunk cutting and rejuvenation, carrying out third trunk cutting treatment before the sea-buckthorn tree body sprouts, wherein the height of the third trunk cutting is marked as H2, and the numerical value of H2 is H < H2 is less than or equal to (H +20) cm, or H > H2 is more than or equal to (H-20) cm;
when the value of H2 is H < H2 ≦ (H +20) cm, 3-5 lateral branches are reserved between H and H2 of the trunk, when the value of H2 is H > H2 ≧ (H-20), 3-5 lateral branches are reserved between (H2-20) to H2, the retention length of the lateral branches is 20-30cm, and then step S2 and step S3 are carried out; h2 is not equal to H1.
4. The method for rejuvenating high sea buckthorn stems according to claim 1, wherein in step S1, the operation time of the stem cutting treatment is early spring, 5-10 days before sea buckthorn sprouts.
5. The high-stem-cutting rejuvenation method for sea buckthorn according to claim 1 wherein in step S2, the amount of the graft wax is 15-20 g/plant.
6. The high-stem-cutting rejuvenation method for sea buckthorn according to claim 1, wherein the wax grafting comprises the following components in parts by weight: 1-2 parts of rosin, 0.5-0.8 part of olive oil and 0.5-1 part of yellow wax.
7. The high-cut-dry rejuvenation method for sea buckthorn according to claim 6, further comprising 0.1 to 0.5 weight part of a tackifier, wherein the tackifier comprises pentaerythritol rosin ester and N, N' -1, 2-ethanediylbisacyl (carbo) amide in a mass ratio of 1:0.3 to 0.5.
8. The high-stem-cutting rejuvenation method for sea buckthorn according to claim 7 wherein said wax further comprises 0.5 to 1 part of a bactericide.
9. The high-stem-cutting rejuvenation method for sea buckthorn according to claim 8, wherein the bactericide is prepared by:
(1) dissolving 1-2 parts by weight of chitosan and 2-4 parts by weight of carboxymethyl chitosan in 10 parts by weight of lactic acid with the concentration of 2%, and uniformly stirring to obtain a chitosan solution;
(2) adding 0.4-1 part by weight of beta-cyclodextrin and 0.5-1 part by weight of sodium benzoate into the chitosan solution obtained in the step (1), and performing ultrasonic dispersion to obtain a coating solution;
(3) 0.4-0.8 part of chinaberry powder, 1-2 parts of pseudolarix powder, 0.3-0.6 part of beta-indoleacetic acid and 1-1.5 parts of hollow mesoporous silica microspheres are uniformly mixed to form sterilization particles, the coating solution is uniformly atomized on the sterilization particles, and freeze drying is carried out to prepare the bactericide, wherein the mass ratio of the coating solution to the sterilization particles is 0.3-0.5: 1.
10. The high-stem-cutting rejuvenation method for sea buckthorn according to claim 9 wherein the graft wax is prepared by the following method: heating olive oil to 65-70 deg.C, adding Colophonium and Cera flava, mixing, heating to 100 deg.C and 110 deg.C, adding tackifier, stirring for 20-30min, adding antibacterial agent, mixing, and cooling to normal temperature to obtain the final product.
CN202110833313.5A 2021-07-22 2021-07-22 High-stem-cutting rejuvenation method for sea buckthorn Pending CN113348951A (en)

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CN101049073A (en) * 2007-05-09 2007-10-10 杜宏彬 Method for clipping fruit tree heavily
JP4665220B1 (en) * 2010-06-24 2011-04-06 稲本マシンツール工業株式会社 Chestnut branching with side branch renewal pruning
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US20150020448A1 (en) * 2012-04-10 2015-01-22 Tae-soo Park Method for forming ornamental tree by pruning and inducing growth of branches
CN107926940A (en) * 2017-11-27 2018-04-20 赤壁神山兴农科技有限公司 A kind of actinidia tree branch special-purpose sealing wax and preparation method thereof
CN109496656A (en) * 2018-12-03 2019-03-22 扬州大学 A method of improving Folium Ginkgo quality and harvest efficiency
CN111501372A (en) * 2020-04-14 2020-08-07 昆山阿基里斯新材料科技有限公司 High-durability antibacterial mildew-proof polyurethane synthetic leather and preparation method thereof

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101049073A (en) * 2007-05-09 2007-10-10 杜宏彬 Method for clipping fruit tree heavily
JP4665220B1 (en) * 2010-06-24 2011-04-06 稲本マシンツール工業株式会社 Chestnut branching with side branch renewal pruning
CN102936485A (en) * 2011-08-16 2013-02-20 江苏泰尔新材料科技有限公司 Wafer adhesive wax
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