CN113016552B - A method for inducing and improving bolting and flowering efficiency of spores mustard - Google Patents
A method for inducing and improving bolting and flowering efficiency of spores mustard Download PDFInfo
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- CN113016552B CN113016552B CN202110287730.4A CN202110287730A CN113016552B CN 113016552 B CN113016552 B CN 113016552B CN 202110287730 A CN202110287730 A CN 202110287730A CN 113016552 B CN113016552 B CN 113016552B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/60—Flowers; Ornamental plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
本发明公开了一种诱导提高抱子芥抽薹开花效率的方法，属于农作物栽培技术领域，包括以下步骤：(1)选用新鲜成熟合格的抱子芥商品种；(2)8月中旬将上述种子置于烧杯中，45℃～50℃温水缓慢冲入，轻缓搅拌3～5分钟，静置3～4小时后，用纱布沥干水；(3)沥水后的种子放入铺有滤纸的高温消毒灭菌的培养皿里，将种子均匀摊开在滤纸上，保存滤纸湿润，用封口膜封口，20℃～25℃暗培养10～14小时，直至80％种子露白；(4)将装有露白种子的培养皿放入3℃～5℃冰箱暗处理10～30天，保持滤纸湿润；(5)将暗处理10～30天的种子与未处理的种子在9月上旬同期播种于50孔穴盘中，25～30天后移栽；大田肥水管理同于正常季节播种的抱子芥生产管理。The invention discloses a method for inducing and improving the bolting and flowering efficiency of spores of mustard, which belongs to the technical field of crop cultivation and includes the following steps: (1) selecting fresh and mature qualified spores of mustard commercial varieties; (2) planting the above seeds in mid-August Place in a beaker, slowly pour in warm water at 45°C to 50°C, stir gently for 3 to 5 minutes, let it sit for 3 to 4 hours, then drain the water with gauze; (3) Place the drained seeds into a pot covered with filter paper. In a high-temperature sterilized petri dish, spread the seeds evenly on the filter paper, keep the filter paper moist, seal it with a sealing film, and cultivate it in the dark at 20°C to 25°C for 10 to 14 hours until 80% of the seeds are white; (4) Place the seeds on the filter paper. The Petri dish with white seeds was darkened in a refrigerator at 3°C to 5°C for 10 to 30 days, keeping the filter paper moist; (5) The seeds that were darkened for 10 to 30 days and the untreated seeds were sown at the same time in early September at 50 In the hole tray, transplant after 25 to 30 days; the management of fertilizer and water in the field is the same as the production management of spores sown in normal seasons.
Description
Technical Field
The invention belongs to the technical field of crop cultivation, and particularly relates to a method for improving flowering efficiency of bolting of arabidopsis thaliana in an induction manner.
Background
The cruciferous plants mainly form a complex network through 6 ways to regulate and control bolting and flowering, namely a photoperiod way, a circadian clock, a vernalization way, an autonomous way, a gibberellin way, an age way and an external temperature way. In brassica vegetable crops, mustard is a typical seed vernalization crop and usually requires a long period of low temperature to be experienced to bolt and flower through vernalization.
The mustard (Brassica junsea var. Gemmifera Lee et Lin.) is a variety of Brassica juncea of Brassicaceae, commonly known as Brassica juncea, etc., and is mainly distributed in southwest region and middle and downstream region of Yangtze river, and is one of important vegetables in winter and spring such as Sichuan province and Chongqing. The winter and spring vegetables are on the market in the off season with high yield and good benefit, and positive contribution is made for guaranteeing urban and rural supply and increasing peasant income.
Most of the arabidopsis thaliana is a biennial cultivated crop, and the arabidopsis thaliana is good in warm, cool and moist environment, suitable for winter and spring cultivation, and strong in growth vigor. The seed of the baby cabbage starts to sprout at 8-10 ℃, the optimal temperature for seedling growth is about 20 ℃, when the baby cabbage grows to a certain stage, the top growing axillary buds start to expand, and enter the period of growing children, and the optimal temperature for axillary bud expansion is 10-15 ℃. The sowing period is a key link of high-yield cultivation of the brassica napus, and the Sichuan basin is sown in the beginning of 8-9 months. Early sowing period is too early, the early period is Wen Tuchang, the plants are bolting-formed into stick shape, and the buds are not growing; the sowing period is too late, the small plants are vernalized at low temperature, the axillary buds are few and small, the commodity shape cannot be normally expressed, and effective breeding work is difficult to develop. The brassica napus is cultivated in normal seasons, axillary buds are enlarged, commodity character observation selection is excellent, but bolting, flowering and fruiting are difficult, and the brassica napus cannot be seen and left, so that the brassica napus cross breeding technology is difficult to break through, and a high-yield high-quality new variety of stress-resistant brassica napus cannot be bred.
Disclosure of Invention
The invention aims to provide a method for improving the flowering efficiency of bolting of arabidopsis thaliana by induction, which solves the technical problems that the prior art has the defects of cultivation of the arabidopsis thaliana in normal seasons, enlarged axillary buds and excellent commodity character observation selection, but the method is difficult to bolt, flower and fruit, can only be seen and can not be left, so that the hybridization breeding technology of the arabidopsis thaliana is difficult to break through, and a new variety of high-yield high-quality stress-resistant arabidopsis thaliana can not be bred.
The invention provides a method for improving flowering efficiency of bolting of arabidopsis thaliana by induction, which comprises the following steps:
(1) Selecting fresh and mature qualified product of Arabidopsis thaliana;
(2) Putting the seeds into a beaker in the middle ten days of 8 months, slowly flushing in warm water at 45-50 ℃, gently stirring for 3-5 minutes, standing for 3-4 hours, and draining with gauze;
(3) Placing the drained seeds into a high-temperature disinfection and sterilization culture dish paved with filter paper, uniformly spreading the seeds on the filter paper, preserving the filter paper for wetting, sealing by a sealing film, and carrying out dark culture for 10-14 hours at 20-25 ℃ until 80% of the seeds are exposed to white;
(4) Placing the culture dish filled with the white seeds into a refrigerator at the temperature of 3-5 ℃ for dark treatment for 10-30 days, and keeping the filter paper moist;
(5) Sowing seeds subjected to dark treatment for 10-30 days on a 50-hole tray in the same period in the last ten days of 9 months, and transplanting for 25-30 days; the field fertilizer and water management is the same as the production management of the arabidopsis thaliana sowed in normal seasons;
(6) Spraying the heart leaf part of the plant with gibberellin 100-500 mg/l for 7 days for 5 times continuously when the plant enters the rosette period;
(7) And (5) normally managing the field until bolting, flowering and fruiting.
Further, the row spacing of the transplanted plants in the step (5) is 50 x 50cm.
Further, gibberellin is 300mg/l in the step (6).
The beneficial effects of the invention are as follows:
1. the method of using 2-5 ℃ to lower Wen Anchu to sprout the seed of the arabidopsis thaliana for 10-30 days and using the plant rosette period to continuously take 5 times, and spraying gibberellin for 7 days is adopted, the bolting time is advanced by about 12 days, the flowering period is advanced by about 15 days, the bolting rate of the arabidopsis thaliana can be improved to 10% -70%, and the setting rate can be improved to 11.3% -35%.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions in the embodiments of the present invention will be clearly and completely described in the following in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments. The components of the embodiments of the invention as generally illustrated may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the invention provided is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, are intended to fall within the scope of the present invention.
In addition, the embodiments of the present invention and the features of the embodiments may be combined with each other without collision.
In the description of the present invention, it should be noted that, directions or positional relationships indicated by terms such as "upper", "lower", "inner", "outer", etc., are based on the directions or positional relationships shown, or directions or positional relationships conventionally put in use of the inventive product, or directions or positional relationships conventionally understood by those skilled in the art are merely for convenience of describing the present invention and simplifying the description, and are not indicative or implying that the apparatus or element to be referred to must have a specific direction, be constructed and operated in a specific direction, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used merely to distinguish between descriptions and should not be construed as indicating or implying relative importance.
In the description of the present invention, it should also be noted that, unless explicitly specified and limited otherwise, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; the two components can be mechanically connected, can be directly connected or can be indirectly connected through an intermediate medium, and can be communicated with each other. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
Example 1
The embodiment of the invention provides a method for improving the flowering efficiency of bolting of arabidopsis thaliana in an induction way, which comprises the following steps:
(1) Selecting fresh and mature qualified product of Arabidopsis thaliana;
(2) Putting the seeds into a beaker in the middle of 8 months, slowly flushing with warm water at 45 ℃, gently stirring for 4 minutes, standing for 4 hours, and draining with gauze;
(3) Placing the drained seeds into a high-temperature disinfection and sterilization culture dish paved with filter paper, uniformly spreading the seeds on the filter paper, preserving the filter paper for wetting, sealing by a sealing film, and carrying out dark culture at 25 ℃ for 10 hours until 80% of the seeds are exposed to white;
(4) Placing the culture dish filled with the white seeds into a refrigerator at 4 ℃ for dark treatment for 20 days, and keeping the filter paper moist;
(5) Sowing seeds subjected to dark treatment for 20 days on a 50-hole tray in the same period of 9 months, transplanting for 25 days, wherein the plant row spacing is 50 x 50cm; the field fertilizer and water management is the same as the production management of the arabidopsis thaliana sowed in normal seasons;
(6) Spraying the heart leaf part of the plant with gibberellin 100mg/l for 7 days for 5 times continuously when the plant enters the rosette period;
(7) And (5) normally managing the field until bolting, flowering and fruiting.
The bolting time is 11 days in advance, the flowering period is 14 days in advance, the bolting rate is 65.0%, and the setting rate is 30.0%.
Example 2
This embodiment, as a preferred example of the present invention, comprises the following steps:
(1) Selecting fresh and mature qualified product of Arabidopsis thaliana;
(2) Putting the seeds into a beaker in the middle of 8 months, slowly flushing with warm water at 45 ℃, gently stirring for 4 minutes, standing for 4 hours, and draining with gauze;
(3) Placing the drained seeds into a high-temperature disinfection and sterilization culture dish paved with filter paper, uniformly spreading the seeds on the filter paper, preserving the filter paper for wetting, sealing by a sealing film, and carrying out dark culture at 24 ℃ for 12 hours until 80% of the seeds are exposed to white;
(4) Placing the culture dish filled with the white seeds into a refrigerator at 4 ℃ for dark treatment for 20 days, and keeping the filter paper moist;
(5) Sowing seeds subjected to dark treatment for 20 days in the same period of 9 months and transplanting the seeds into a 50-hole tray for 25 days, wherein the plant row spacing is 50cm; the field fertilizer and water management is the same as the production management of the arabidopsis thaliana sowed in normal seasons;
(6) Spraying 300mg/l gibberellin on the heart leaf part of the plant for 7 days for 5 times continuously when the plant enters the rosette period;
(7) And (5) normally managing the field until bolting, flowering and fruiting.
The bolting time is 12 days in advance, the flowering period is 15 days in advance, the bolting rate is 70%, and the setting rate is 35.0%.
Example 3
This embodiment, as a preferred example of the present invention, comprises the following steps:
(1) Selecting fresh and mature qualified product of Arabidopsis thaliana;
(2) Putting the seeds into a beaker in the middle of 8 months, slowly flushing with warm water at 50 ℃, gently stirring for 5 minutes, standing for 4 hours, and draining with gauze;
(3) Placing the drained seeds into a high-temperature disinfection and sterilization culture dish paved with filter paper, uniformly spreading the seeds on the filter paper, preserving the filter paper for wetting, sealing by a sealing film, and carrying out dark culture at 25 ℃ for 14 hours until 80% of the seeds are exposed to white;
(4) Placing the culture dish filled with the white seeds into a refrigerator at 4 ℃ for dark treatment for 20 days, and keeping the filter paper moist;
(5) Sowing seeds subjected to dark treatment for 20 days in the same period of 9 months and a 50-hole tray, transplanting the seeds for 30 days, wherein the plant row spacing is 50 x 50cm; the field fertilizer and water management is the same as the production management of the arabidopsis thaliana sowed in normal seasons;
(6) Spraying 500mg/l gibberellin on the heart leaf part of the plant for 7 days for 5 times continuously when the plant enters the rosette period;
(7) And (5) normally managing the field until bolting, flowering and fruiting.
The bolting time is 12 days in advance, the flowering period is 14 days in advance, the bolting rate is 50.0%, and the setting rate is 20.0%.
Example 4
This embodiment, as a control group of the present invention, includes the steps of:
(1) Normal temperature drying and placing;
(2) Spraying the heart leaf part of the plant with clear water in the last ten days of 11 months, wherein the spraying is carried out for 5 times in 7 days;
(3) And (5) normally managing the field until bolting, flowering and fruiting.
The bolting time is not advanced, the flowering period is not advanced, the bolting rate is 10.4%, and the setting rate is 4.2%.
Statistical table of bolting plant rate and setting rate in the above embodiment
Claims (3)
1. The method for improving flowering efficiency of bolting of the arabidopsis thaliana by induction is characterized by comprising the following steps of:
(1) Selecting fresh and mature qualified commodity seeds of the arabidopsis thaliana;
(2) Putting the seeds into a beaker in the middle ten days of 8 months, slowly flushing in warm water at 45-50 ℃, gently stirring for 3-5 minutes, standing for 3-4 hours, and draining with gauze;
(3) Placing the drained seeds into a high-temperature disinfection and sterilization culture dish paved with filter paper, uniformly spreading the seeds on the filter paper, preserving the filter paper for wetting, sealing by a sealing film, and carrying out dark culture for 10-14 hours at 20-25 ℃ until 80% of the seeds are exposed to white;
(4) Placing the culture dish filled with the white seeds into a refrigerator at the temperature of 2-5 ℃ for dark treatment for 10-30 days, and keeping the filter paper moist;
(5) Sowing seeds subjected to dark treatment for 10-30 days on a 50-hole tray in the same period in the last ten days of 9 months, and transplanting for 25-30 days; the field fertilizer and water management is the same as the production management of the arabidopsis thaliana sowed in normal seasons;
(6) Spraying the heart leaf part of the plant with gibberellin 100-500 mg/L for 7 days for 5 times continuously when the plant enters the rosette period;
(7) And (5) normally managing the field until bolting, flowering and fruiting.
2. The method for improving flowering efficiency of bolting in arabidopsis thaliana according to claim 1, wherein the row spacing of the transplanted plant in the step (5) is 50 x 50cm.
3. The method for improving flowering efficiency of bolting in arabidopsis thaliana according to claim 1 or 2, wherein gibberellin is 300mg/L in the step (6).
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