CN113016552B - Method for improving flowering efficiency of bolting of arabidopsis thaliana by induction - Google Patents
Method for improving flowering efficiency of bolting of arabidopsis thaliana by induction Download PDFInfo
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- CN113016552B CN113016552B CN202110287730.4A CN202110287730A CN113016552B CN 113016552 B CN113016552 B CN 113016552B CN 202110287730 A CN202110287730 A CN 202110287730A CN 113016552 B CN113016552 B CN 113016552B
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- 241000219195 Arabidopsis thaliana Species 0.000 title claims abstract description 27
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title claims abstract description 12
- 230000006698 induction Effects 0.000 title claims abstract description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 238000007789 sealing Methods 0.000 claims abstract description 12
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 12
- 238000009331 sowing Methods 0.000 claims abstract description 9
- 239000003337 fertilizer Substances 0.000 claims abstract description 6
- 238000011010 flushing procedure Methods 0.000 claims abstract description 6
- 238000004519 manufacturing process Methods 0.000 claims abstract description 6
- 230000001954 sterilising effect Effects 0.000 claims abstract description 6
- 238000003756 stirring Methods 0.000 claims abstract description 6
- 238000009736 wetting Methods 0.000 claims abstract description 6
- 241000196324 Embryophyta Species 0.000 claims description 21
- 229930191978 Gibberellin Natural products 0.000 claims description 9
- IXORZMNAPKEEDV-UHFFFAOYSA-N gibberellic acid GA3 Natural products OC(=O)C1C2(C3)CC(=C)C3(O)CCC2C2(C=CC3O)C1C3(C)C(=O)O2 IXORZMNAPKEEDV-UHFFFAOYSA-N 0.000 claims description 9
- 239000003448 gibberellin Substances 0.000 claims description 9
- 238000005507 spraying Methods 0.000 claims description 8
- 241000758794 Asarum Species 0.000 claims description 6
- 240000002791 Brassica napus Species 0.000 description 5
- 235000011293 Brassica napus Nutrition 0.000 description 5
- 241000219198 Brassica Species 0.000 description 4
- 235000013311 vegetables Nutrition 0.000 description 3
- 101100438273 Arabidopsis thaliana CAN1 gene Proteins 0.000 description 2
- 235000011331 Brassica Nutrition 0.000 description 2
- 235000003351 Brassica cretica Nutrition 0.000 description 2
- 244000178993 Brassica juncea Species 0.000 description 2
- 235000011332 Brassica juncea Nutrition 0.000 description 2
- 235000014700 Brassica juncea var napiformis Nutrition 0.000 description 2
- 240000007124 Brassica oleracea Species 0.000 description 2
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 2
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 2
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 2
- 235000003343 Brassica rupestris Nutrition 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- QKSKPIVNLNLAAV-UHFFFAOYSA-N bis(2-chloroethyl) sulfide Chemical compound ClCCSCCCl QKSKPIVNLNLAAV-UHFFFAOYSA-N 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 235000010460 mustard Nutrition 0.000 description 2
- 241000219193 Brassicaceae Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000026287 bud dilation involved in lung branching Effects 0.000 description 1
- 230000008632 circadian clock Effects 0.000 description 1
- 238000009402 cross-breeding Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/60—Flowers; Ornamental plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Botany (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Soil Sciences (AREA)
- Cultivation Of Plants (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a method for improving flowering efficiency of bolting of arabidopsis thaliana by induction, which belongs to the technical field of crop cultivation and comprises the following steps: selecting fresh and mature qualified product of Arabidopsis thaliana; (2) Putting the seeds into a beaker in the middle ten days of 8 months, slowly flushing in warm water at 45-50 ℃, gently stirring for 3-5 minutes, standing for 3-4 hours, and draining with gauze; (3) Placing the drained seeds into a high-temperature disinfection and sterilization culture dish paved with filter paper, uniformly spreading the seeds on the filter paper, preserving the filter paper for wetting, sealing by a sealing film, and carrying out dark culture for 10-14 hours at 20-25 ℃ until 80% of the seeds are exposed to white; (4) Placing the culture dish filled with the white seeds into a refrigerator at the temperature of 3-5 ℃ for dark treatment for 10-30 days, and keeping the filter paper moist; (5) Sowing seeds subjected to dark treatment for 10-30 days and untreated seeds in a 50-hole tray in the same period in the last 9 months, and transplanting after 25-30 days; the field fertilizer and water management is the same as the production management of the arabidopsis thaliana sowed in normal seasons.
Description
Technical Field
The invention belongs to the technical field of crop cultivation, and particularly relates to a method for improving flowering efficiency of bolting of arabidopsis thaliana in an induction manner.
Background
The cruciferous plants mainly form a complex network through 6 ways to regulate and control bolting and flowering, namely a photoperiod way, a circadian clock, a vernalization way, an autonomous way, a gibberellin way, an age way and an external temperature way. In brassica vegetable crops, mustard is a typical seed vernalization crop and usually requires a long period of low temperature to be experienced to bolt and flower through vernalization.
The mustard (Brassica junsea var. Gemmifera Lee et Lin.) is a variety of Brassica juncea of Brassicaceae, commonly known as Brassica juncea, etc., and is mainly distributed in southwest region and middle and downstream region of Yangtze river, and is one of important vegetables in winter and spring such as Sichuan province and Chongqing. The winter and spring vegetables are on the market in the off season with high yield and good benefit, and positive contribution is made for guaranteeing urban and rural supply and increasing peasant income.
Most of the arabidopsis thaliana is a biennial cultivated crop, and the arabidopsis thaliana is good in warm, cool and moist environment, suitable for winter and spring cultivation, and strong in growth vigor. The seed of the baby cabbage starts to sprout at 8-10 ℃, the optimal temperature for seedling growth is about 20 ℃, when the baby cabbage grows to a certain stage, the top growing axillary buds start to expand, and enter the period of growing children, and the optimal temperature for axillary bud expansion is 10-15 ℃. The sowing period is a key link of high-yield cultivation of the brassica napus, and the Sichuan basin is sown in the beginning of 8-9 months. Early sowing period is too early, the early period is Wen Tuchang, the plants are bolting-formed into stick shape, and the buds are not growing; the sowing period is too late, the small plants are vernalized at low temperature, the axillary buds are few and small, the commodity shape cannot be normally expressed, and effective breeding work is difficult to develop. The brassica napus is cultivated in normal seasons, axillary buds are enlarged, commodity character observation selection is excellent, but bolting, flowering and fruiting are difficult, and the brassica napus cannot be seen and left, so that the brassica napus cross breeding technology is difficult to break through, and a high-yield high-quality new variety of stress-resistant brassica napus cannot be bred.
Disclosure of Invention
The invention aims to provide a method for improving the flowering efficiency of bolting of arabidopsis thaliana by induction, which solves the technical problems that the prior art has the defects of cultivation of the arabidopsis thaliana in normal seasons, enlarged axillary buds and excellent commodity character observation selection, but the method is difficult to bolt, flower and fruit, can only be seen and can not be left, so that the hybridization breeding technology of the arabidopsis thaliana is difficult to break through, and a new variety of high-yield high-quality stress-resistant arabidopsis thaliana can not be bred.
The invention provides a method for improving flowering efficiency of bolting of arabidopsis thaliana by induction, which comprises the following steps:
(1) Selecting fresh and mature qualified product of Arabidopsis thaliana;
(2) Putting the seeds into a beaker in the middle ten days of 8 months, slowly flushing in warm water at 45-50 ℃, gently stirring for 3-5 minutes, standing for 3-4 hours, and draining with gauze;
(3) Placing the drained seeds into a high-temperature disinfection and sterilization culture dish paved with filter paper, uniformly spreading the seeds on the filter paper, preserving the filter paper for wetting, sealing by a sealing film, and carrying out dark culture for 10-14 hours at 20-25 ℃ until 80% of the seeds are exposed to white;
(4) Placing the culture dish filled with the white seeds into a refrigerator at the temperature of 3-5 ℃ for dark treatment for 10-30 days, and keeping the filter paper moist;
(5) Sowing seeds subjected to dark treatment for 10-30 days on a 50-hole tray in the same period in the last ten days of 9 months, and transplanting for 25-30 days; the field fertilizer and water management is the same as the production management of the arabidopsis thaliana sowed in normal seasons;
(6) Spraying the heart leaf part of the plant with gibberellin 100-500 mg/l for 7 days for 5 times continuously when the plant enters the rosette period;
(7) And (5) normally managing the field until bolting, flowering and fruiting.
Further, the row spacing of the transplanted plants in the step (5) is 50 x 50cm.
Further, gibberellin is 300mg/l in the step (6).
The beneficial effects of the invention are as follows:
1. the method of using 2-5 ℃ to lower Wen Anchu to sprout the seed of the arabidopsis thaliana for 10-30 days and using the plant rosette period to continuously take 5 times, and spraying gibberellin for 7 days is adopted, the bolting time is advanced by about 12 days, the flowering period is advanced by about 15 days, the bolting rate of the arabidopsis thaliana can be improved to 10% -70%, and the setting rate can be improved to 11.3% -35%.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions in the embodiments of the present invention will be clearly and completely described in the following in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments. The components of the embodiments of the invention as generally illustrated may be arranged and designed in a wide variety of different configurations.
Thus, the following detailed description of the embodiments of the invention provided is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, are intended to fall within the scope of the present invention.
In addition, the embodiments of the present invention and the features of the embodiments may be combined with each other without collision.
In the description of the present invention, it should be noted that, directions or positional relationships indicated by terms such as "upper", "lower", "inner", "outer", etc., are based on the directions or positional relationships shown, or directions or positional relationships conventionally put in use of the inventive product, or directions or positional relationships conventionally understood by those skilled in the art are merely for convenience of describing the present invention and simplifying the description, and are not indicative or implying that the apparatus or element to be referred to must have a specific direction, be constructed and operated in a specific direction, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used merely to distinguish between descriptions and should not be construed as indicating or implying relative importance.
In the description of the present invention, it should also be noted that, unless explicitly specified and limited otherwise, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; the two components can be mechanically connected, can be directly connected or can be indirectly connected through an intermediate medium, and can be communicated with each other. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
Example 1
The embodiment of the invention provides a method for improving the flowering efficiency of bolting of arabidopsis thaliana in an induction way, which comprises the following steps:
(1) Selecting fresh and mature qualified product of Arabidopsis thaliana;
(2) Putting the seeds into a beaker in the middle of 8 months, slowly flushing with warm water at 45 ℃, gently stirring for 4 minutes, standing for 4 hours, and draining with gauze;
(3) Placing the drained seeds into a high-temperature disinfection and sterilization culture dish paved with filter paper, uniformly spreading the seeds on the filter paper, preserving the filter paper for wetting, sealing by a sealing film, and carrying out dark culture at 25 ℃ for 10 hours until 80% of the seeds are exposed to white;
(4) Placing the culture dish filled with the white seeds into a refrigerator at 4 ℃ for dark treatment for 20 days, and keeping the filter paper moist;
(5) Sowing seeds subjected to dark treatment for 20 days on a 50-hole tray in the same period of 9 months, transplanting for 25 days, wherein the plant row spacing is 50 x 50cm; the field fertilizer and water management is the same as the production management of the arabidopsis thaliana sowed in normal seasons;
(6) Spraying the heart leaf part of the plant with gibberellin 100mg/l for 7 days for 5 times continuously when the plant enters the rosette period;
(7) And (5) normally managing the field until bolting, flowering and fruiting.
The bolting time is 11 days in advance, the flowering period is 14 days in advance, the bolting rate is 65.0%, and the setting rate is 30.0%.
Example 2
This embodiment, as a preferred example of the present invention, comprises the following steps:
(1) Selecting fresh and mature qualified product of Arabidopsis thaliana;
(2) Putting the seeds into a beaker in the middle of 8 months, slowly flushing with warm water at 45 ℃, gently stirring for 4 minutes, standing for 4 hours, and draining with gauze;
(3) Placing the drained seeds into a high-temperature disinfection and sterilization culture dish paved with filter paper, uniformly spreading the seeds on the filter paper, preserving the filter paper for wetting, sealing by a sealing film, and carrying out dark culture at 24 ℃ for 12 hours until 80% of the seeds are exposed to white;
(4) Placing the culture dish filled with the white seeds into a refrigerator at 4 ℃ for dark treatment for 20 days, and keeping the filter paper moist;
(5) Sowing seeds subjected to dark treatment for 20 days in the same period of 9 months and transplanting the seeds into a 50-hole tray for 25 days, wherein the plant row spacing is 50cm; the field fertilizer and water management is the same as the production management of the arabidopsis thaliana sowed in normal seasons;
(6) Spraying 300mg/l gibberellin on the heart leaf part of the plant for 7 days for 5 times continuously when the plant enters the rosette period;
(7) And (5) normally managing the field until bolting, flowering and fruiting.
The bolting time is 12 days in advance, the flowering period is 15 days in advance, the bolting rate is 70%, and the setting rate is 35.0%.
Example 3
This embodiment, as a preferred example of the present invention, comprises the following steps:
(1) Selecting fresh and mature qualified product of Arabidopsis thaliana;
(2) Putting the seeds into a beaker in the middle of 8 months, slowly flushing with warm water at 50 ℃, gently stirring for 5 minutes, standing for 4 hours, and draining with gauze;
(3) Placing the drained seeds into a high-temperature disinfection and sterilization culture dish paved with filter paper, uniformly spreading the seeds on the filter paper, preserving the filter paper for wetting, sealing by a sealing film, and carrying out dark culture at 25 ℃ for 14 hours until 80% of the seeds are exposed to white;
(4) Placing the culture dish filled with the white seeds into a refrigerator at 4 ℃ for dark treatment for 20 days, and keeping the filter paper moist;
(5) Sowing seeds subjected to dark treatment for 20 days in the same period of 9 months and a 50-hole tray, transplanting the seeds for 30 days, wherein the plant row spacing is 50 x 50cm; the field fertilizer and water management is the same as the production management of the arabidopsis thaliana sowed in normal seasons;
(6) Spraying 500mg/l gibberellin on the heart leaf part of the plant for 7 days for 5 times continuously when the plant enters the rosette period;
(7) And (5) normally managing the field until bolting, flowering and fruiting.
The bolting time is 12 days in advance, the flowering period is 14 days in advance, the bolting rate is 50.0%, and the setting rate is 20.0%.
Example 4
This embodiment, as a control group of the present invention, includes the steps of:
(1) Normal temperature drying and placing;
(2) Spraying the heart leaf part of the plant with clear water in the last ten days of 11 months, wherein the spraying is carried out for 5 times in 7 days;
(3) And (5) normally managing the field until bolting, flowering and fruiting.
The bolting time is not advanced, the flowering period is not advanced, the bolting rate is 10.4%, and the setting rate is 4.2%.
Statistical table of bolting plant rate and setting rate in the above embodiment
Claims (3)
1. The method for improving flowering efficiency of bolting of the arabidopsis thaliana by induction is characterized by comprising the following steps of:
(1) Selecting fresh and mature qualified commodity seeds of the arabidopsis thaliana;
(2) Putting the seeds into a beaker in the middle ten days of 8 months, slowly flushing in warm water at 45-50 ℃, gently stirring for 3-5 minutes, standing for 3-4 hours, and draining with gauze;
(3) Placing the drained seeds into a high-temperature disinfection and sterilization culture dish paved with filter paper, uniformly spreading the seeds on the filter paper, preserving the filter paper for wetting, sealing by a sealing film, and carrying out dark culture for 10-14 hours at 20-25 ℃ until 80% of the seeds are exposed to white;
(4) Placing the culture dish filled with the white seeds into a refrigerator at the temperature of 2-5 ℃ for dark treatment for 10-30 days, and keeping the filter paper moist;
(5) Sowing seeds subjected to dark treatment for 10-30 days on a 50-hole tray in the same period in the last ten days of 9 months, and transplanting for 25-30 days; the field fertilizer and water management is the same as the production management of the arabidopsis thaliana sowed in normal seasons;
(6) Spraying the heart leaf part of the plant with gibberellin 100-500 mg/L for 7 days for 5 times continuously when the plant enters the rosette period;
(7) And (5) normally managing the field until bolting, flowering and fruiting.
2. The method for improving flowering efficiency of bolting in arabidopsis thaliana according to claim 1, wherein the row spacing of the transplanted plant in the step (5) is 50 x 50cm.
3. The method for improving flowering efficiency of bolting in arabidopsis thaliana according to claim 1 or 2, wherein gibberellin is 300mg/L in the step (6).
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110287730.4A CN113016552B (en) | 2021-03-17 | 2021-03-17 | Method for improving flowering efficiency of bolting of arabidopsis thaliana by induction |
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| CN202110287730.4A CN113016552B (en) | 2021-03-17 | 2021-03-17 | Method for improving flowering efficiency of bolting of arabidopsis thaliana by induction |
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| CN113016552B true CN113016552B (en) | 2024-03-12 |
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| CN110301336A (en) * | 2019-07-25 | 2019-10-08 | 福建省中科生物股份有限公司 | A kind of plant factor's cultural method of Stem lettuce |
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