CN113005079A - 一种人骨髓间充质干细胞体外扩增用添加剂及扩增方法 - Google Patents
一种人骨髓间充质干细胞体外扩增用添加剂及扩增方法 Download PDFInfo
- Publication number
- CN113005079A CN113005079A CN202110499065.5A CN202110499065A CN113005079A CN 113005079 A CN113005079 A CN 113005079A CN 202110499065 A CN202110499065 A CN 202110499065A CN 113005079 A CN113005079 A CN 113005079A
- Authority
- CN
- China
- Prior art keywords
- mesenchymal stem
- stem cells
- additive
- amplification
- culture medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0663—Bone marrow mesenchymal stem cells (BM-MSC)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/998—Proteins not provided for elsewhere
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Developmental Biology & Embryology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Rheumatology (AREA)
- Hematology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
组别 | 增殖倍数 | 细胞活率 |
实施例1 | 34.79 | 98.41% |
实施例2 | 32.84 | 97.58% |
实施例3 | 33.98 | 98.04% |
对比例1 | 18.36 | 76.83% |
对比例2 | 15.32 | 74.06% |
对比例3 | 14.79 | 72.59% |
对比例4 | 16.02 | 75.33% |
对比例5 | 21.49 | 81.79% |
对比例6 | 23.79 | 84.93% |
对比例7 | 19.87 | 80.08% |
对比例8 | 18.72 | 79.36% |
样品 | 钙化面积(%) |
实施例1 | 72.31±4.14 |
实施例2 | 70.57±4.25 |
实施例3 | 72.17±4.12 |
对比例1 | 54.19±3.68 |
对比例2 | 52.46±3.91 |
对比例3 | 58.02±3.85 |
对比例4 | 61.71±4.31 |
对比例5 | 45.37±3.27 |
对比例6 | 48.95±4.06 |
对比例7 | 44.65±3.79 |
对比例8 | 45.12±3.90 |
Claims (9)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202110499065.5A CN113005079B (zh) | 2021-05-08 | 2021-05-08 | 一种人骨髓间充质干细胞体外扩增用添加剂及扩增方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202110499065.5A CN113005079B (zh) | 2021-05-08 | 2021-05-08 | 一种人骨髓间充质干细胞体外扩增用添加剂及扩增方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN113005079A true CN113005079A (zh) | 2021-06-22 |
CN113005079B CN113005079B (zh) | 2022-12-06 |
Family
ID=76380672
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202110499065.5A Active CN113005079B (zh) | 2021-05-08 | 2021-05-08 | 一种人骨髓间充质干细胞体外扩增用添加剂及扩增方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN113005079B (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114574435A (zh) * | 2022-02-22 | 2022-06-03 | 岳俊丽 | 一种诱导脐带间充质干细胞分泌细胞因子的组合物及其应用 |
Citations (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101412985A (zh) * | 2007-10-15 | 2009-04-22 | 华东理工大学 | 用于骨髓间充质干细胞体外培养和扩增的无血清培养基 |
CN102550416A (zh) * | 2012-01-10 | 2012-07-11 | 江西师范大学 | 产石杉碱甲的离体蛇足石杉叶状体诱导和增殖的培养方法 |
CN102978156A (zh) * | 2012-11-13 | 2013-03-20 | 湖州市中心医院 | 一种骨髓间充质干细胞的体外扩增纯化培养方法及培养基 |
CN103667182A (zh) * | 2012-12-17 | 2014-03-26 | 湖州市中心医院 | 一种骨髓间充质干细胞在体外向成骨细胞分化的诱导方法及诱导培养基 |
WO2015056982A1 (ko) * | 2013-10-17 | 2015-04-23 | (주)아모레퍼시픽 | 만능 줄기세포의 유도 방법 및 상기 방법에 의해 제조된 만능줄기세포 |
WO2016104911A1 (ko) * | 2014-12-23 | 2016-06-30 | 영남대학교 산학협력단 | 엑소솜을 유효성분으로 함유하는 세포 노화 예방 또는 치료용 약학조성물 |
CN106282105A (zh) * | 2016-08-13 | 2017-01-04 | 浙江译美生物科技有限公司 | 一种间充质干细胞培养基 |
CN106635978A (zh) * | 2016-12-21 | 2017-05-10 | 广东科玮生物技术股份有限公司 | 一种脐带间充质干细胞无血清培养基及其制备方法和用途 |
CN106754677A (zh) * | 2016-12-24 | 2017-05-31 | 严志海 | 一种体外骨髓间充质干细胞培养基 |
CN109161525A (zh) * | 2018-10-06 | 2019-01-08 | 淮安康宜莱干细胞生物科技有限公司 | 促进人骨髓间充质干细胞增殖的方法 |
CN110331130A (zh) * | 2019-07-03 | 2019-10-15 | 依科赛生物科技(太仓)有限公司 | 一种间充质干细胞无血清培养基及其用途 |
CN110468101A (zh) * | 2019-09-18 | 2019-11-19 | 安徽科门生物科技有限公司 | 一种骨髓间充质干细胞的增殖培养方法 |
CN111621476A (zh) * | 2020-05-12 | 2020-09-04 | 河南侨创生命科技有限公司 | 一种用于间充质干细胞的无血清培养基及其制备方法 |
CN112080463A (zh) * | 2020-09-25 | 2020-12-15 | 郑州佐爵生物科技有限公司 | 一种促进骨髓间充质干细胞成骨分化的方法 |
CN112094812A (zh) * | 2020-09-25 | 2020-12-18 | 郑州佐爵生物科技有限公司 | 一种促进骨髓间充质干细胞向神经元细胞分化的方法 |
CN112322580A (zh) * | 2017-04-28 | 2021-02-05 | 北京赛斯达生物技术有限公司 | 一种间充质干细胞无血清培养基的应用 |
-
2021
- 2021-05-08 CN CN202110499065.5A patent/CN113005079B/zh active Active
Patent Citations (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101412985A (zh) * | 2007-10-15 | 2009-04-22 | 华东理工大学 | 用于骨髓间充质干细胞体外培养和扩增的无血清培养基 |
CN102550416A (zh) * | 2012-01-10 | 2012-07-11 | 江西师范大学 | 产石杉碱甲的离体蛇足石杉叶状体诱导和增殖的培养方法 |
CN102978156A (zh) * | 2012-11-13 | 2013-03-20 | 湖州市中心医院 | 一种骨髓间充质干细胞的体外扩增纯化培养方法及培养基 |
CN103667182A (zh) * | 2012-12-17 | 2014-03-26 | 湖州市中心医院 | 一种骨髓间充质干细胞在体外向成骨细胞分化的诱导方法及诱导培养基 |
WO2015056982A1 (ko) * | 2013-10-17 | 2015-04-23 | (주)아모레퍼시픽 | 만능 줄기세포의 유도 방법 및 상기 방법에 의해 제조된 만능줄기세포 |
WO2016104911A1 (ko) * | 2014-12-23 | 2016-06-30 | 영남대학교 산학협력단 | 엑소솜을 유효성분으로 함유하는 세포 노화 예방 또는 치료용 약학조성물 |
CN106282105A (zh) * | 2016-08-13 | 2017-01-04 | 浙江译美生物科技有限公司 | 一种间充质干细胞培养基 |
CN106635978A (zh) * | 2016-12-21 | 2017-05-10 | 广东科玮生物技术股份有限公司 | 一种脐带间充质干细胞无血清培养基及其制备方法和用途 |
CN106754677A (zh) * | 2016-12-24 | 2017-05-31 | 严志海 | 一种体外骨髓间充质干细胞培养基 |
CN112322580A (zh) * | 2017-04-28 | 2021-02-05 | 北京赛斯达生物技术有限公司 | 一种间充质干细胞无血清培养基的应用 |
CN109161525A (zh) * | 2018-10-06 | 2019-01-08 | 淮安康宜莱干细胞生物科技有限公司 | 促进人骨髓间充质干细胞增殖的方法 |
CN110331130A (zh) * | 2019-07-03 | 2019-10-15 | 依科赛生物科技(太仓)有限公司 | 一种间充质干细胞无血清培养基及其用途 |
CN110468101A (zh) * | 2019-09-18 | 2019-11-19 | 安徽科门生物科技有限公司 | 一种骨髓间充质干细胞的增殖培养方法 |
CN111621476A (zh) * | 2020-05-12 | 2020-09-04 | 河南侨创生命科技有限公司 | 一种用于间充质干细胞的无血清培养基及其制备方法 |
CN112080463A (zh) * | 2020-09-25 | 2020-12-15 | 郑州佐爵生物科技有限公司 | 一种促进骨髓间充质干细胞成骨分化的方法 |
CN112094812A (zh) * | 2020-09-25 | 2020-12-18 | 郑州佐爵生物科技有限公司 | 一种促进骨髓间充质干细胞向神经元细胞分化的方法 |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114574435A (zh) * | 2022-02-22 | 2022-06-03 | 岳俊丽 | 一种诱导脐带间充质干细胞分泌细胞因子的组合物及其应用 |
Also Published As
Publication number | Publication date |
---|---|
CN113005079B (zh) | 2022-12-06 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN110923196B (zh) | 无血清培养基及其制备方法和间充质干细胞的培养方法 | |
CN109762782B (zh) | 一种促间充质干细胞生长的培养基及其制备方法 | |
CN104805054A (zh) | 干细胞无血清培养基 | |
CN112662624A (zh) | 一种用于骨髓间充质干细胞体外培养和扩增的无血清培养基 | |
CN113736729B (zh) | 组合物及含有其的干细胞无血清培养基及干细胞培养方法 | |
CN111154718B (zh) | 一种人间充质干细胞体外快速扩增用添加剂及扩增方法 | |
CN111621476B (zh) | 一种用于间充质干细胞的无血清培养基及其制备方法 | |
CN105039248B (zh) | 树鼩骨髓间充质干细胞培养系统 | |
CN114480273A (zh) | 用于获得间充质干细胞及其外泌体的培养基及其制备方法 | |
CN104928247A (zh) | 一种神经干细胞培养基及神经干细胞贴壁培养方法 | |
CN106754675A (zh) | 一种脂肪干细胞无血清培养基及其制备方法和用途 | |
CN113151165B (zh) | 一种人脐带间充质干细胞扩增用培养基及培养方法 | |
CN113005079B (zh) | 一种人骨髓间充质干细胞体外扩增用添加剂及扩增方法 | |
CN104774808A (zh) | 将脐带间充质干细胞诱导分化成γ-氨基丁酸能神经元的方法 | |
CN110699317A (zh) | 一种人脐带间充质干细胞无血清培养基及其制备方法与应用 | |
CN116694567A (zh) | 将人诱导性多能干细胞间接诱导为间充质干细胞的培养液和方法 | |
CN114438025B (zh) | 一种制备脂肪间充质干细胞因子的诱导培养基及培养方法 | |
CN113652396B (zh) | 一种脂肪间充质干细胞成骨诱导组合物及成骨诱导方法 | |
Shipounova et al. | Hierarchy of mesenchymal stem cells: Comparison of multipotentmesenchymal stromal cells with fibroblast colony forming units | |
CN110628712B (zh) | 基于诱导多能干细胞的治疗级间充质干细胞制备方法及应用 | |
CN112708598A (zh) | 无血清成分的神经前体细胞培养基及其配制方法和应用 | |
CN111849900A (zh) | 一种自体脂肪MSCs来源的前体细胞智能培养方法 | |
CN104774807A (zh) | 将脐带间充质干细胞诱导分化成少突胶质细胞的方法 | |
CN111304163A (zh) | 脐带间充质干细胞的规模化培养方法 | |
KR102435452B1 (ko) | 노화가 감소되고 줄기세포능이 보존된 초기 중간엽 줄기세포, 및 그 배양방법 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
TA01 | Transfer of patent application right | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20221116 Address after: 264203 12th floor, office building of Weihai Zhengsheng Biotechnology Co., Ltd., north of Huanghe street and east of Guangtai airport, Zhangcun Town, Huancui District, Weihai City, Shandong Province Applicant after: Weihai Jiansheng Biotechnology Co.,Ltd. Address before: 052165 No.8, Huahua North Road, qiutou village, qiutou Town, Shijiazhuang circular chemical industry park, Shijiazhuang City, Hebei Province Applicant before: Hebei Chixi Technology Development Co.,Ltd. |
|
GR01 | Patent grant | ||
GR01 | Patent grant | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: An additive and amplification method for in vitro expansion of human bone marrow mesenchymal stem cells Effective date of registration: 20230425 Granted publication date: 20221206 Pledgee: Industrial and Commercial Bank of China Limited Weihai Branch Pledgor: Weihai Jiansheng Biotechnology Co.,Ltd. Registration number: Y2023980039089 |