CN112655390A - Cuttage and rapid propagation method for akebia quinata - Google Patents
Cuttage and rapid propagation method for akebia quinata Download PDFInfo
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- CN112655390A CN112655390A CN202011482026.6A CN202011482026A CN112655390A CN 112655390 A CN112655390 A CN 112655390A CN 202011482026 A CN202011482026 A CN 202011482026A CN 112655390 A CN112655390 A CN 112655390A
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Abstract
The invention provides a cutting propagation method of clematis chinensis, which is characterized in that a proper clematis chinensis cutting matrix is found, a reasonable matrix component distribution ratio is set, and through the matching of various cutting propagation details such as a corresponding proper cutting depth, the number of nodes of cutting slips, the number of leaves, growth hormones with proper soaking concentration and components and the like, the rooting efficiency and the survival rate are improved, the propagation efficiency of clematis chinensis is improved, the propagation yield of clematis chinensis is favorably improved, the production cost is reduced, and the requirements of expanded propagation production and landscape application of clematis chinensis resources are met.
Description
Technical Field
The invention belongs to the technical field of plant propagation, and particularly relates to a cutting propagation method of clematis armandii.
Background
Caulis Akebiae, Clematis Armandii, Ranunculaceae, is a evergreen vine. Cylindrical stem, three-fold leaves, leathery leaf, oval long ellipse. In the flowering period of 3-4 months, the cymbidium floridum is gathered, the diameter of a single flower is about 2cm, the flower color is mostly milk white, the color is light pink, and the flower quantity is large. The fruit period is 4-7 months, the lean fruit is flat, and white hair is grown and soft. Mainly distributed in Yunnan, Guizhou, Sichuan, Hubei, Hunan, etc. At present, natural propagation in original land is mainly relied on.
The clematis chinensis is large in flower quantity and strong in adaptability, is an excellent early-flowering evergreen clematis, is scarce in market quantity and is to be popularized and applied. However, most of the seeds produced by selfing of caulis akebiae in east China have empty shells and low fruiting rate, and the seed sowing method has low propagation yield and low speed, and cannot meet the requirement of large-scale production in the market.
In order to solve the problems, the xylocarpa usually adopts cutting propagation, and the conventional method comprises the following steps: the length of the cutting slips is generally 10-15 cm, at least 3-4 internodes are needed, the lower leaves are cut off, 2 leaves are left at the top end, and the bottom is cut into a 45-degree inclined plane by a sharp quick cutter. After the cutting slips are cut, the 2cm position of the base parts of the cutting slips is soaked in 0.005-0.01% naphthylacetic acid water solution, and after 24 hours, the cutting slips can be taken out for cutting propagation. Before cuttage, a place which faces the sun and the lee needs to be selected to manufacture a seedbed. Preferably loose, permeable and drained soil, after deep ploughing and raking, arranging the box according to the length of 500 plus 700 cm and the width of 80-100 cm to make a bed, then filling loose and permeable yellow sandy soil with the thickness of 35-40 cm on the bed, sterilizing the seedbed by 3000 times of potassium permanganate aqueous solution after the seedbed is made, sealing for one day and night, and airing for several days to make cuttage possible. When cutting, the prepared cutting is cut according to the plant-row spacing of 15-25 cm, the depth of the soil is 2/3 of the length of the cutting, and a section is generally reserved. During operation, the bamboo stick is used for punching, the cutting is inserted, the soil is covered and compacted, and water is sprayed once to ensure that the cutting and the matrix are tightly attached. After cuttage of the clematis filamentosa, a pergola needs to be built on a seedbed, and generally, the illumination intensity is only 45%. The substrate is kept moist, and water can be sprayed to the leaf surface every day in a mist manner, so that the evaporation of water is reduced, and the leaves are kept fresh and green. The temperature of the seedbed is controlled at 20-25 ℃, and if the temperature is too high, ventilation and water spraying cooling are also enhanced. After ten days and a half month, the ground temperature can be increased to 23-28 ℃, the seedbed is not watered, and the phenomenon that the ground temperature is increased due to excessive moisture, so that healing tissues are rotted, and the cutting shoots are rooted is prevented. When buds begin to sprout, the illumination intensity needs to be properly increased to strengthen the water management. After the sprouts sprout true leaves, the sprouts can be sprayed with 0.2-0.3% urea aqueous solution once every ten days and a half month, and can also be sprayed with 0.2% monopotassium phosphate aqueous solution alternately to promote the growth of the seedlings.
However, the conventional common cutting method needs a large amount of cutting shoots, requires a long time for pretreatment in cutting, has the problems of low rooting efficiency and low survival rate, has low propagation yield and low speed, and still cannot well meet the requirement of large-scale production in the market.
Disclosure of Invention
Based on the above, the invention aims to provide the rapid cutting propagation method of the clematis filamentosa, the clematis filamentosa produced by the method has high yield and low production cost, and meets the requirements of propagation production and landscape application of clematis filamentosa resources. The specific technical scheme is as follows:
the cutting method of the clematis chinensis bunge comprises the following steps:
(1) preparation of a matrix: adopting perlite, vermiculite and peat according to the proportion of 1: (0.9-1.1): (0.9-1.2) preparing a cutting medium; placing a cutting medium into the planting disc, wherein the thickness of the cutting medium is 12cm-16 cm;
(2) cutting treatment: cutting the semi-lignified branch of akebia quinata as cutting slips, reserving 1-2 nodes and half to one leaf of each cutting slip, and cutting off the rest leaves; soaking the cutting slips in a growth hormone solution and then cutting the cutting slips in the substrate in the step (1); the growth hormone is prepared by mixing naphthylacetic acid and indolebutyric acid according to the proportion of 1 (0.8-1.1), and the concentration of the growth hormone solution is 50-150 ppm;
(3) managing after the insertion: placing the cutting seedling in a shady environment with the temperature of 15-20 ℃ and the humidity of 60-80%; maintaining the substrate moisture at about 50-70%.
In some of these embodiments, the cutting medium is composed of perlite, vermiculite, peat in a 1: (0.9-1): (1-1.1) preparation.
In some of these embodiments, the cutting medium is composed of perlite, vermiculite, peat in a 1:1: (1-1.1) preparation.
In some of these embodiments, the cutting retains 2 nodes and half to one leaf.
In some of these embodiments, the cutting is soaked in the growth hormone solution for 0.5 to 1.5 hours.
In some of the examples, the growth hormone solution is prepared by mixing naphthylacetic acid and indolebutyric acid according to the proportion of 1 (1-1.1).
In some of these embodiments, the cutting is soaked in the growth hormone solution for 0.8 to 1.2 hours.
In some embodiments, the cutting depth in the substrate in the cutting step (1) after the cutting slips are soaked in the growth hormone solution in the step (2) is 4.5-5.5 cm; and/or
The cutting distance is (4cm-6cm) multiplied by (4cm-6 cm).
In some embodiments, after the cutting substrate is prepared in step (1), the substrate is drenched with a bactericide for disinfection before the cutting in step (2); and/or
After cuttage, spraying a bactericide on the cuttage seedlings.
In some of these examples, the cuttings were transplanted into pots after rooting was observed 50-100 days after cutting.
Based on the technical scheme, the invention has the following beneficial effects:
according to the invention, a proper cutting matrix of the clematis filamentosa dunn is found, a reasonable matrix component proportion is set, and through the matching of various cutting propagation details such as the corresponding proper cutting depth, the number of nodes of cutting slips, the number of leaves, growth hormone with proper soaking concentration and components and the like, the rooting efficiency and the survival rate are improved, the propagation efficiency of the clematis filamentosa dunn is improved, the propagation yield of the clematis filamentosa dunn is favorably improved, the production cost is reduced, and the requirements of expanding propagation production and landscape application of clematis filamentosa dunn resources are met.
Drawings
FIG. 1 shows the rooting of Akebia quinata by cutting according to the scheme described in example 1.
FIG. 2 shows the situation of cutting and rooting of Akebia quinata after the treatment by the method described in example 2.
FIG. 3 shows the situation of cutting and rooting of Akebia quinata after the treatment by the method described in example 3.
FIG. 4 shows the root of Akebia quinata after being treated by the method described in example 4.
FIG. 5 shows the condition of cutting and rooting of Akebia quinata after the treatment by the method described in comparative example 1.
FIG. 6 shows the root of Akebia quinata after the cuttage according to the comparative example 2.
FIG. 7 shows the condition of cutting and rooting of Akebia quinata after the treatment by the method described in comparative example 3.
Detailed Description
In order that the invention may be more readily understood, reference will now be made to the following more particular description of the invention, examples of which are set forth below. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. These embodiments are provided so that this disclosure will be thorough and complete. It is to be understood that the experimental procedures in the following examples, where specific conditions are not noted, are generally in accordance with conventional conditions, or with conditions recommended by the manufacturer. The various reagents used in the examples are commercially available.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
The cutting method of the akebia quinata provided by the invention comprises the following steps of:
(1) preparation of a matrix: adopting perlite, vermiculite and peat according to the proportion of 1: (0.9-1.1): (0.9-1.2) preparing a cutting medium; placing a cutting medium into the planting disc, wherein the thickness of the cutting medium is 12cm-16 cm;
(2) cutting treatment: cutting the semi-lignified branch of akebia quinata as cutting slips, reserving 1-2 nodes and half to one leaf of each cutting slip, and cutting off the rest leaves; soaking the cutting slips in a growth hormone solution and then cutting the cutting slips in the substrate in the step (1); the growth hormone is prepared by mixing naphthylacetic acid and indolebutyric acid according to the proportion of 1 (0.8-1.1);
(3) managing after the insertion: placing the cutting seedling in a shady environment with the temperature of 15-20 ℃ and the humidity of 60-80%; maintaining the substrate moisture at about 50-70%.
Preferably, the cutting medium is prepared from perlite, vermiculite and peat according to the ratio of 1: (0.9-1): (1-1.1) preparation. It may be 1:0.9:1 or 1:0.9:1.1 or 1:0.9:1.05 or 1:1:1.1 and in the range of 1: (0.9-1): (1-1.1) in any ratio. More preferably, the cutting medium is prepared from perlite, vermiculite and peat according to the ratio of 1:1: (1-1.1) preparation. Further preferably, the cutting medium is prepared from perlite, vermiculite and peat according to the ratio of 1:1:1, preparing the mixture. In some of these examples, the thickness of the substrate is guaranteed to be between 12 and 16 cm. As the akebia quinata is a fibrous root system and does not resist damp, heat and accumulated water, the substrate should be soft and has good air permeability. The substrate used in the invention is the mixture of perlite, vermiculite and peat, the perlite can make the substrate light and breathable, the vermiculite can improve the breathability and the water content of the substrate, the peat is rich in organic matters, the three are mixed according to the proportion, the balance of moisture retention, breathability and nutrition can be achieved, the substrate which is soft, breathable, moisture retention and rich in organic nutrients is formed, the requirements of the fibrous root of the akebia stem on moisture, breathability, nutrients and the like are met, the fibrous root growth is facilitated, the root rot is prevented, and the transplanting into a container after the rooting is facilitated.
Preferably, the cutting retains 2 nodes and half to one leaf. More preferably, the cutting retains 2 nodes and half leaves. The buds and leaves of the nodes can produce certain nutrient substances through photosynthesis and produce certain amount of hormone for rooting of the cutting slips, and the number of the nodes and the leaves has important influence on the rooting efficiency and survival rate of the cutting. If the nodes are too many, the long cuttings and the too deep soil are inevitably caused by too many nodes due to the long internodes of the akebia quinata, and the roots are easy to wet with water and insufficient in air permeability, so that the rooting is not facilitated; if too many leaves are reserved, the cuttings are too dehydrated too much due to the fact that the leaves of akebia trifoliata are large and excessive transpiration is caused, and the leaves are not reserved, photosynthesis is limited, nutrients of the cuttings are insufficient, and unfavorable rooting is caused.
Preferably, the cutting is soaked in the growth hormone solution for 0.5 to 1.5 hours. More preferably, the cutting is soaked in the growth hormone solution for 0.8 to 1.2 hours. More preferably, the cutting slips are soaked in the growth hormone solution for 0.9 to 1.1 hours.
Preferably, the growth hormone solution is prepared by mixing naphthylacetic acid and indolebutyric acid according to the proportion of 1 (1-1.1). More preferably, the growth hormone solution is prepared by mixing naphthylacetic acid and indolebutyric acid according to the proportion of 1:1.
Preferably, the growth hormone solution has a concentration of 50-150 ppm. Preferably 80-120 ppm. More preferably 90 to 110 ppm.
In some embodiments, the cutting slips are soaked in growth hormone solution prepared by mixing naphthylacetic acid and indolebutyric acid according to the proportion of 1 (1-1.1) at the concentration of 90-110ppm for 0.9-1.1 hours.
In fact, the length of time for which the cutting slips are soaked in growth hormone, and the components, concentration and proportion of the growth hormone can affect the cutting propagation efficiency of the clematis chinensis cutting slips. For example, a certain concentration of growth hormone can promote plant rooting, and an excessively high concentration can inhibit plant growth and reduce the rooting rate of cuttings.
In some embodiments, the cutting depth in the substrate in the cutting step (1) after the cutting slips are soaked in the growth hormone solution in the step (2) is 4.5-5.5 cm. Too deep cutting can result in excessive moisture in the roots, insufficient ventilation, and unfavorable rooting.
In some of these embodiments, the cutting spacing is (4cm-6cm) × (4cm-6 cm). Preferably (4.5cm-5.5cm) × (4.5cm-5.5 cm).
In some of these embodiments, after the cutting substrate is prepared in step (1), the substrate is drenched with a bactericide for sterilization before the cutting in step (2). Alternatively, the fungicide may be a carbendazim solution, a potassium permanganate solution or any other fungicide having a killing effect on bacteria or fungi that can cause crop diseases. Preferably a carbendazim solution. In some of the embodiments, the carbendazim solution needs to be diluted for use, and the preferred dilution factor is 600-1000. More preferably 700-900 times.
Similarly, after cutting, the cuttings are sprayed with a bactericide. The bactericide sprayed to the cutting seedlings can be a carbendazim solution, a potassium permanganate solution or any other bactericide with a killing effect on bacteria or fungi capable of causing crop diseases. Preferably a carbendazim solution. In some of the embodiments, the carbendazim solution needs to be diluted for use, and the preferred dilution factor is 600-1000. More preferably 700-900 times.
In some of these examples, the cuttings were transplanted into pots after rooting was observed 50-100 days after cutting. Preferably 70-90 days. More preferably, transplanting is performed when the number of roots per cutting is observed to be 3 or more.
Preferably, the transplanting is carried out when the average root length is more than 4 cm.
Furthermore, transplanting when the root number of each cutting shoot reaches more than 4 and the average root length reaches more than 5 cm.
The invention is described in detail below by way of examples:
example 1
(1) Preparation of substrate/cutting bed: perlite, vermiculite and peat are adopted, and the weight ratio is 1:1:1, preparing a cutting medium. Placing the cutting medium in a planting disc, wherein the thickness of the medium is about 12-16 cm. And (3) irrigating the matrix thoroughly by using 800 times of carbendazim solution, and disinfecting.
(2) Cutting treatment: and in the 9 months, cutting a semi-lignified branch grown in the current year of akebia quinata to serve as a cutting, reserving 2 nodes and half leaves for each cutting, and cutting off the rest leaves. And (3) soaking the cutting slips in a growth hormone solution for 1 hour, and then cutting the cutting slips in the prepared substrate. The growth hormone is prepared by mixing naphthylacetic acid and indolebutyric acid according to the proportion of 1:1, and the concentration of the growth hormone solution is 100 ppm. The cutting depth is 4.5-5.5cm, and the cutting distance is 5cm x 5 cm.
(3) Managing after the insertion: placing the cutting seedling in a shady environment with the temperature of 15-20 ℃ and the humidity of 60-80%. Regularly spraying water on the cuttage container, and keeping the humidity of the substrate to be about 50-70%. Spraying with 800-fold diluted carbendazim solution every 2 weeks.
(4) Transplanting and potting: after 70-90 days of cutting, when the root number of each cutting shoot reaches more than 4 and the average root length reaches more than 5cm, transplanting into a 1 gallon planting container.
In this example, after the clematis filamentosa dunn is cut, the rooting rate is counted to be 85% on the 90 th day, the average number of rooting strips of each cutting shoot is 6.1 strips/shoot, and the average number of root length is 9.8 cm. The rooting condition is shown in figure 1.
Example 2
This example is substantially the same as the cutting propagation method described in example 1, and the main differences are only: the growth hormone used in this example was prepared by mixing naphthylacetic acid and indolebutyric acid at a ratio of 1:1, and the concentration was 150 ppm.
In this example, after the clematis filamentosa is subjected to cuttage, the rooting rate is 75% by statistics on day 90, the average rooting number is 6.4 pieces/piece, and the average root length is 7.8 cm. The rooting is shown in figure 2.
Example 3
This example is substantially the same as the cutting propagation method described in example 1, and the main differences are only: each cutting ear of this example has 2 nodes. Whereas 1 node was retained per cutting in example 1.
In this example, after the clematis filamentosa dunn is cut, the rooting rate is 67% on day 90, the average root length is 6.9cm, and the average number of roots per cutting shoot is 4.6. The rooting rate was slower than in example 1. The rooting is shown in figure 3.
Example 4
This example is substantially the same as the cutting propagation method described in example 1, and the main differences are only: in this example, each cutting has 2 knots, and the growth hormone of this example is prepared by mixing naphthylacetic acid and indolebutyric acid at a ratio of 1:0.9, and the concentration of the growth hormone solution is 120 ppm.
In this example, after the clematis filamentosa dunn is cut, the rooting rate is 75% by statistics on day 90, the average root length is 5.7cm, and the average root number of each cutting shoot is 4.7. The rooting is shown in figure 4.
Comparative example 1
This example is substantially the same as the cutting propagation method described in example 1, and the main differences are only: the substrate used in this example was perlite and vermiculite mixed in a volume ratio of 1:1.
In this example, after the clematis filamentosa is subjected to cuttage, the rooting rate is counted to be 30% on the 90 th day, the average rooting number is 4.8 pieces/piece, and the average root length is 7.9 cm. The rooting is shown in figure 5.
Comparative example 2
This comparative example is essentially the same as the cutting propagation method described in example 1, with the main differences being only: the growth hormone of the comparative example is prepared by mixing naphthylacetic acid and indolebutyric acid according to the proportion of 1:1, and the concentration of the growth hormone solution is 200 ppm. The concentration of growth hormone was significantly higher than in example 1.
After the clematis chinensis is cut in the comparative example, the rooting rate is 53 percent, the average root length is 6.8 and the average root number of each cutting shoot is 4.5 on day 90. The rooting is shown in figure 6.
Comparative example 3
This comparative example is essentially the same as the cutting propagation method described in example 1, with the main differences being only:
the growth hormone of this comparative example was a 100ppm aqueous solution of naphthylacetic acid.
After the clematis chinensis is cut in the comparative example, the rooting rate is counted to be 37.5 percent on the 90 th day, the average root length is 5.9, and the average root number of each cutting shoot is 4.2. The rooting is shown in FIG. 7.
The technical features of the above embodiments can be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the above embodiments are not described, however, as long as there is no contradiction between the combinations of the technical features, the scope of the present description should be considered as being described in the present specification.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. The cutting method of the clematis chinensis is characterized by comprising the following steps:
(1) preparation of a matrix: adopting perlite, vermiculite and peat according to the proportion of 1: (0.9-1.1): (0.9-1.2) preparing a cutting medium; placing a cutting medium into the planting disc, wherein the thickness of the cutting medium is 12cm-16 cm;
(2) cutting treatment: cutting the semi-lignified branch of akebia quinata as cutting slips, reserving 1-2 nodes and half to one leaf of each cutting slip, and cutting off the rest leaves; soaking the cutting slips in a growth hormone solution and then cutting the cutting slips in the substrate in the step (1); the growth hormone is prepared by mixing naphthylacetic acid and indolebutyric acid according to the proportion of 1 (0.8-1.1), and the concentration of the growth hormone solution is 50-150 ppm;
(3) managing after the insertion: placing the cutting seedling in a shady environment with the temperature of 15-20 ℃ and the humidity of 60-80%; maintaining the substrate moisture at about 50-70%.
2. The cutting method of akebia quinata as claimed in claim 1, wherein the cutting medium is formed by perlite, vermiculite and peat according to a ratio of 1: (0.9-1): (1-1.1) preparation.
3. The cutting method of akebia quinata as claimed in claim 2, wherein the cutting medium is formed by perlite, vermiculite and peat according to a ratio of 1:1: (1-1.1) preparation.
4. The cutting method of akebia quinata according to claim 1, wherein the cutting shoot retains 2 nodes and half to one leaf.
5. The cutting method of akebia quinata according to claim 1, wherein the cutting shoots are soaked in the growth hormone solution for 0.5-1.5 hours.
6. The cutting method of akebia quinata as claimed in claim 5, wherein the growth hormone solution is prepared by mixing naphthylacetic acid and indolebutyric acid according to the proportion of 1 (1-1.1).
7. The cutting method of akebia quinata according to claim 5 or 6, wherein the cutting shoots are soaked in the growth hormone solution for 0.8-1.2 hours.
8. The cutting method of akebia quinata according to any one of claims 1 to 6, wherein the cutting depth in the substrate of the cutting step (1) after the cutting slips are soaked in the growth hormone solution in the step (2) is 4.5-5.5 cm; and/or
The cutting distance is (4cm-6cm) multiplied by (4cm-6 cm).
9. The cutting method of akebia quinata according to any one of claims 1-6, wherein after the cutting substrate is prepared in step (1), the substrate is drenched with a bactericide for sterilization before the cutting in step (2); and/or
After cuttage, spraying a bactericide on the cuttage seedlings.
10. The cutting method of caulis akebiae according to any one of claims 1 to 6, wherein after 50 to 100 days of cutting, rooting of the cutting shoot is observed, and the cutting shoot is transplanted into a pot.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114982491A (en) * | 2022-07-08 | 2022-09-02 | 湖南省植物园 | Cutting propagation method of monomer camellia japonica |
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