CN112175093B

CN112175093B - 对鳞翅目害虫具有毒性或抑制性的嵌合杀昆虫蛋白质

Info

Publication number: CN112175093B
Application number: CN202011054128.8A
Authority: CN
Inventors: J·A·鲍姆; T·A·塞鲁蒂; C·L·达特; L·H·恩格里斯; 付晓然; V·M·古佐夫; A·R·豪; J·P·摩根斯特恩; J·K·罗伯茨; S·A·萨尔瓦多; 王金玲; S·弗拉辛斯基
Original assignee: Monsanto Technology LLC
Current assignee: Monsanto Technology LLC
Priority date: 2014-10-16
Filing date: 2015-10-15
Publication date: 2024-08-27
Anticipated expiration: 2035-10-15
Also published as: KR102208985B1; ZA202001771B; EA201892763A1; NZ768150A; ES2864657T3; CA3151125A1; BR112017007794B1; US10611806B2; IL251570A0; CA2964776A1; CU24551B1; KR20190142455A; US10494408B2; CU20170049A7; JP6626102B2; KR102127553B1; CN112142857A; EP3207049A2; WO2016061391A2; CR20210268A

Abstract

公开了编码新型嵌合杀昆虫蛋白质的核苷酸序列，所述嵌合杀昆虫蛋白质表现出鳞翅目抑制活性。具体实施方案提供了含有编码一种或多种所述嵌合杀昆虫蛋白质的重组核酸分子的组合物以及经过转化的植物、植物部分和种子。

Description

对鳞翅目害虫具有毒性或抑制性的嵌合杀昆虫蛋白质

本申请是申请日为2015年10月15日、申请号为201580055840.0、发明名称为“对鳞翅目害虫具有毒性或抑制性的新型嵌合杀昆虫蛋白质”的发明专利申请的分案申请。

对相关申请的引用

本申请要求2014年10月16日提交的美国临时申请No.62/064,989的权益，该申请以全文引用的方式并入本文中。

序列表的并入

序列表的计算机可读形式与本申请一起通过电子提交方式提交并且以全文引用的方式并入本申请。序列表包含在2016年8月23日创建的文件中，具有文件名P34230WO00_Seq_PCT_Art34.txt，并且大小是898,229字节(如在操作系统中所测量)。

发明领域

本发明总体上涉及昆虫抑制性蛋白质领域。在本申请中公开了对农作物和种子的农业相关害虫表现出昆虫抑制性活性的一类新型的嵌合杀昆虫蛋白质。具体来说，所公开的类别的蛋白质对鳞翅目昆虫害虫表现出了杀昆虫活性。提供了含有编码所公开的毒素蛋白质中的一种或多种的重组核酸分子的植物、植物部分和种子。

发明背景

提高重要农业植物，尤其包括玉米、大豆、甘蔗、水稻、小麦、蔬菜和棉花的作物产量已经变得越来越重要。除了日益增长的人口对用于提供食物、衣物和能量的农产品的需要日益增长以外，预计气候相关的影响和来自于日益增长的人口将土地用于除农业耕作以外的用途的压力也会减少可耕种耕地的量。这些因素已经导致了关于粮食保障的严峻预测，特别是在植物生物技术和农业技术不存在重大改进的情况下。鉴于这些压力，技术、农业技术和害虫治理的环境可持续改进是在有限量的可耕种耕地上使作物增产的重要工具。

昆虫，特别是鳞翅目内的昆虫，被认为是破坏农田作物，从而降低受侵扰区域中的作物产量的主要原因。会对农业造成负面影响的鳞翅目害虫物种包括但不限于草地粘虫(草地贪夜蛾)、甜菜粘虫(甜菜夜蛾)、披肩粘虫(蓓带夜蛾)、黑切根虫(球菜夜蛾)、甘蓝尺蠖(粉纹夜蛾)、大豆尺蠖(大豆夜蛾)、黎豆毛虫(黎豆夜蛾)、绿叶虫(苜蓿绿夜蛾)、烟草芽虫(烟芽夜蛾)、颗粒切根虫(黄地老虎)、粘虫(一星粘虫)、西方切根虫(灰地老虎)、欧洲玉米钻心虫(欧洲玉米螟)、脐橙虫(脐橙螟)、玉米根结网虫(玉米根网螟)、草地结网虫(水稻切叶野螟)、向日葵蛾(向日葵螟)、小玉米茎钻心虫(南美玉米苗斑螟)、苹果蛾(苹果蠹蛾)、葡萄果蛾(葡萄小食心虫)、东方果蛾(梨小食心虫)、向日葵芽小卷蛾(向日葵芽卷叶蛾)、钻背蛾(小菜蛾)、粉红棉铃虫(棉红铃虫)、粉红钻茎虫(大螟)、吉普赛蛾(舞毒蛾)、棉叶虫(棉叶波纹夜蛾)、果树卷叶虫(果树黄卷蛾)、欧洲卷叶虫(玫瑰黄卷蛾)、亚洲水稻钻心虫或水稻钻茎虫(二化螟)、水稻卷叶虫(稻纵卷叶螟)、玉米根结网虫(玉米根网螟)、蓝草结网虫(早熟禾草螟)、西南玉米钻心虫(西南玉米螟)、蔗螟(小蔗螟)、多刺棉铃虫(埃及金刚钻)、斑点棉铃虫(翠纹金刚钻)、古棉铃虫(棉铃实夜蛾)、玉米穗虫、大豆荚虫或棉铃虫(玉米穗夜蛾)、草地结网虫(水稻切叶野螟)、欧洲葡萄蛾(欧洲葡萄缀穗蛾)、柑桔潜叶虫(柑桔潜叶蛾)、大白蝴蝶(大菜粉蛾)、菜青虫或小白蝴蝶(小菜粉蛾)、烟草切根虫或茶蚕(斜纹夜蛾)和番茄潜叶虫(番茄潜叶蛾)。

在历史上，农业依赖于密集施用作为害虫防治剂的合成化学杀昆虫剂。除了出现抗性问题以外，对环境和人类健康的忧虑也刺激了生物杀虫剂的研究和开发。这种研究工作导致逐渐发现和使用多种昆虫病原微生物物种，包括细菌。

当发现了昆虫病原细菌，尤其是属于芽孢杆菌属的细菌，并且将其开发为生物害虫防治剂时，生物防治模式发生了变化。细菌苏云金芽孢杆菌(Bt)的菌株已经被用作杀昆虫蛋白质的来源，因为人们发现Bt菌株会对特定的昆虫显示出高毒性。已知Bt菌株会在孢子形成开始时和在稳定生长期期间产生位于伴孢结晶包涵体内的δ内毒素(例如Cry蛋白)，而且还已知其能产生分泌型杀昆虫蛋白质。在被易感昆虫摄取后，δ内毒素以及分泌型毒素在中肠皮膜细胞表面发挥其作用，从而破坏细胞膜，导致细胞破环和死亡。除Bt以外的细菌物种中也鉴别出了编码杀昆虫蛋白质的基因，所述细菌物种包括其他芽孢杆菌和多种其他细菌物种，诸如侧孢短芽孢杆菌(Brevibacillus laterosporus)、球形赖氨酸芽孢杆菌(Lysinibacillus sphaericus)(“Ls”先前被称为球形芽孢杆菌(Bacillus sphaericus))和日本甲虫芽孢杆菌(Paenibacillus popilliae)。

结晶型和分泌型可溶性杀昆虫蛋白质毒素对其宿主具有高度特异性，并且已经得以在世界范围内被接受作为化学杀昆虫剂的替代物。举例来说，杀昆虫毒素蛋白质已经被用于多种农业应用中，以防止重要农业植物遭到昆虫侵扰，减少对化学杀虫剂施用的需要和增加产量。通过机械方法诸如将含有多种细菌菌株的微生物制剂分散至植物表面上的喷雾和通过使用旨在产生表达杀昆虫毒素蛋白质的转基因植物和种子的基因转化技术将杀昆虫毒素蛋白质用来防治作物植物的农业相关害虫。

表达杀昆虫蛋白质的转基因植物的使用已经在全世界得到了采用。举例来说，在2012年，有2610万公顷种植了表达Bt毒素的转基因作物(James,C.,Global Status ofCommercialized Biotech/GM Crops:2012.ISAAA Brief第44期)。转基因昆虫防护作物的全球使用和被用于这些作物中的杀昆虫蛋白质的有限数目已经对能赋予针对目前所利用的杀昆虫蛋白质的抗性的现有昆虫等位基因产生了选择压力。

目标害虫中发展对杀昆虫蛋白质的抗性引起持续需要发现和开发可用于控制昆虫对表达杀昆虫蛋白质的转基因作物的抗性增加的杀昆虫蛋白质新形式。具有提高的效力并且对更宽范围的易感昆虫物种表现出控制的新杀昆虫蛋白质将减少可能发展抗性等位基因的存活昆虫的数目。另外，在一种植物中使用两种或更多种对同一昆虫害虫有毒并且呈现出不同的作用模式的转基因杀昆虫蛋白质能降低任何单一目标昆虫物种中出现抗性的概率。

因此，极度需要鉴别出具有改善的杀昆虫性质，诸如与农业耕作中目前使用的毒素相比对更宽范围的目标昆虫害虫物种和不同的作用模式的功效有所增加的其他杀昆虫蛋白质。为了满足这个需要，本发明公开了对主要目标鳞翅目害虫物种表现出活性的新型Cry1嵌合杀昆虫蛋白质。

本领域中已知Cry1晶体蛋白质家族成员会对鳞翅目害虫表现出生物活性。Cry1晶体蛋白质的前体形式由两个近似相等尺寸的片段组成。所述前体蛋白质的羧基末端部分，称为原毒素片段，能使晶体形成稳定并且不表现杀昆虫活性。所述前体蛋白质的胺基末端部分包含Cry1蛋白的毒素片段，并且基于Cry1家族成员内的保守或基本保守序列的比对，可以进一步再分成三个结构域，即结构域I、结构域II和结构域III。结构域I包含活性毒素片段的约前三分之一，而且已经被证明对通道形成是不可或缺的。结构域II和结构域III都牵涉受体结合和昆虫物种特异性，这取决于所研究的昆虫和杀昆虫蛋白质。

由对本领域中已知的众多天然杀昆虫蛋白质的结构域结构进行拣选来任意地产生具有增强的嵌合蛋白质的可能性微乎其微。这是蛋白质结构、寡聚和释放杀昆虫蛋白质片段所需的激活(包括对嵌合前体进行正确蛋白水解处理，如果以此种形式表达的话)的复杂特性的结果。只有通过小心地选择各亲本蛋白质内的原毒素和具体靶标来产生嵌合结构才可能构建出与得到嵌合体的亲本蛋白质相比表现出有所改善的杀昆虫活性的功能嵌合杀昆虫毒素。本领域中已知，重新组装原毒素与任何两种或更多种彼此不同的毒素的毒素结构域I、结构域II和结构域III往往会构建出表现出错误晶体形成或完全缺乏针对优选目标昆虫害虫物种的任何可检测杀昆虫活性的蛋白质。只有通过试错法才能设计出有效的杀昆虫嵌合体，而且即使那样，熟练的技术人员最后也不一定能得到与可能得到嵌合体组成原毒素或毒素结构域的任何单个亲本毒素蛋白质相比表现出等效或改善的杀昆虫活性的嵌合体。举例来说，文献报告了由两种或更多种晶体蛋白质前体构建或组装嵌合蛋白质的众多实例。参见例如Jacqueline S.Knight等人，“A Strategy for Shuffling NumerousBacillus thuringiensis Crystal Protein Domains.”J.Economic Entomology，97(6)(2004)：1805-1813；Bosch等人(美国专利No.6,204,246)；Malvar和Gilmer(美国专利No.6,017,534)。在这些实例中的每一个中，所得嵌合体中有许多与得到嵌合体组分的前体蛋白质相比不能表现出等效或有所改善的杀昆虫或晶体形成性质。

发明概述

提供了编码对鳞翅类植物害虫具有毒性的嵌合杀昆虫蛋白质的重组核酸分子。每一种嵌合杀昆虫蛋白质都可以单独使用或与彼此以及与其他杀昆虫蛋白质和昆虫抑制剂组合于制剂和活体中；从而提供农业系统中目前使用的杀昆虫蛋白质和杀昆虫化学物质的替代物。

在某些实施方案中，本文中公开了一种嵌合杀昆虫蛋白质，其包含如SEQ ID NO：21、10、28、7、4、13、16、19、23、25、30、33、36、39、41、43、45、47、50、53、55、57、59、61、63、65、67、69、71、73、75、77、79、81、83、85、87、89、91、93、95、97、99、101、103、105、107、109或111中的任意者中所示出的氨基酸序列。这种嵌合杀昆虫蛋白质对鳞翅目昆虫物种表现出抑制活性，所述鳞翅目昆虫物种诸如但不限于黎豆夜蛾、小蔗螟、南美玉米苗斑螟、玉米穗夜蛾、烟芽夜蛾、大豆夜蛾、考斯夜蛾、亚热带粘虫、草地贪夜蛾、甜菜夜蛾、棉铃实夜蛾、斜纹夜蛾、棉红铃虫、西南玉米螟、翠纹金刚钻、南美棉铃虫和薄荷灰夜蛾。

在另一个实施方案中，公开了一种编码嵌合杀昆虫蛋白质的多核苷酸，其中所述多核苷酸可操作地连接至异源启动子，并且所述嵌合杀昆虫蛋白质包含如SEQ ID NO:21、10、28、7、4、13、16、19、23、25、30、33、36、39、41、43、45、47、50、53、55、57、59、61、63、65、67、69、71、73、75、77、79、81、83、85、87、89、91、93、95、97、99、101、103、105、107、109或111中的任意者中所示出的氨基酸序列。还设想了一种编码嵌合杀昆虫蛋白质的多核苷酸，其中所述多核苷酸包含的核苷酸序列任选地：在严格条件下与如SEQ ID NO:1、2、3、5、6、8、9、11、12、14、15、17、18、20、22、24、26、27、29、31、32、34、35、37、38、40、42、44、46、48、49、51、52、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128、129或130中的任意者中所示出的多核苷酸序列的反向互补序列杂交；或编码包含如SEQ ID NO:21、10、28、7、4、13、16、19、23、25、30、33、36、39、41、43、45、47、50、53、55、57、59、61、63、65、67、69、71、73、75、77、79、81、83、85、87、89、91、93、95、97、99、101、103、105、107、109或111中的任意者中所示出的氨基酸序列的嵌合杀昆虫蛋白质。

在其他实施方案中，本文中公开了一种宿主细胞，其包含SEQ ID NO:1、2、3、5、6、8、9、11、12、14、15、17、18、20、22、24、26、27、29、31、32、34、35、37、38、40、42、44、46、48、49、51、52、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128、129或130中的任意者中所示出的多核苷酸，其中所述宿主细胞是选自由细菌宿主细胞或植物宿主细胞组成的群组。所设想的细菌宿主包括土壤杆菌、根瘤菌、芽孢杆菌、短芽孢杆菌、埃希氏杆菌、假单胞菌、克雷伯氏杆菌和欧文氏菌；并且其中所述芽孢杆菌属是蜡样芽胞杆菌或苏云金芽孢杆菌，所述短芽孢杆菌是侧孢短芽孢杆菌，且所述埃希氏杆菌是大肠埃希氏杆菌。所设想的植物细胞包括单子叶植物和双子叶植物。

本文中所公开的其他实施方案包括昆虫抑制性组合物，其包含有包含如SEQ IDNO:21、10、28、7、4、13、16、19、23、25、30、33、36、39、41、43、45、47、50、53、55、57、59、61、63、65、67、69、71、73、75、77、79、81、83、85、87、89、91、93、95、97、99、101、103、105、107、109或111中的任意者中所示出的氨基酸序列的嵌合杀昆虫蛋白质。在某些实施方案中，所述昆虫抑制性组合物还包含与所述嵌合杀昆虫蛋白质不同的至少一种昆虫抑制剂。所设想的与所述嵌合杀昆虫蛋白质不同的昆虫抑制剂包括昆虫抑制性蛋白质、昆虫抑制性dsRNA分子和昆虫抑制性化学物质。这些与所述嵌合杀昆虫蛋白质不同的昆虫抑制剂可以对鳞翅目、鞘翅目、半翅目、同翅目或缨翅目的一种或多种害虫物种表现出活性。

在又另一个实施方案中，本文中公开了一种种子，其包含昆虫抑制有效量的以下各物：包含如SEQ ID NO:21、10、28、7、4、13、16、19、23、25、30、33、36、39、41、43、45、47、50、53、55、57、59、61、63、65、67、69、71、73、75、77、79、81、83、85、87、89、91、93、95、97、99、101、103、105、107、109或111中的任意者中所示出的氨基酸序列的嵌合杀昆虫蛋白质；或SEQ IDNO:1、2、3、5、6、8、9、11、12、14、15、17、18、20、22、24、26、27、29、31、32、34、35、37、38、40、42、44、46、48、49、51、52、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128、129或130中的任意者中所示出的多核苷酸。

还设想了一种防治鳞翅目害虫的方法，所述方法包括使所述鳞翅目害虫与抑制量的本发明嵌合杀昆虫蛋白质接触。

在另一个实施方案中，本文中公开了一种转基因植物细胞、植物或植物部分，其包含嵌合杀昆虫蛋白质，其中：所述嵌合杀昆虫蛋白质包含如SEQ ID NO:21、10、28、7、4、13、16、19、23、25、30、33、36、39、41、43、45、47、50、53、55、57、59、61、63、65、67、69、71、73、75、77、79、81、83、85、87、89、91、93、95、97、99、101、103、105、107、109或111中的任意者中所示出的任意氨基酸序列；或所述嵌合杀昆虫蛋白质包含如下蛋白质：与SEQ ID NO:21、10具有至少94％同一性；与SEQ ID NO:28具有至少93％同一性；与SEQ ID NO:7具有至少87％同一性；与SEQ ID NO:4具有至少90％同一性；与SEQ ID NO:13具有至少91％同一性；与SEQ IDNO:16具有至少64％同一性；与SEQ ID NO:19具有至少66％同一性；与SEQ ID NO:23具有至少86％同一性；与SEQ ID NO:25具有至少91％同一性；与SEQ ID NO:30具有至少94％同一性；与SEQ ID NO:33具有至少91％同一性；与SEQ ID NO:36具有至少64％同一性；与SEQ IDNO:39具有至少66％同一性；与SEQ ID NO:41具有至少94％同一性；与SEQ ID NO:43具有至少84％同一性；与SEQ ID NO:45具有至少93％同一性；与SEQ ID NO:47具有至少94％同一性；与SEQ ID NO:50具有至少91％同一性；或与SEQ ID NO:53具有至少93％同一性；或与SEQ ID NO:85、93、105具有至少87％同一性；或与SEQ ID NO:55、57、59、61、63、65、67、69、71、73、75、77、79具有至少85％同一性；或与SEQ ID NO:91、87、89具有至少88％同一性；或与SEQ ID NO:107、111具有至少89％同一性；或与SEQ ID NO:97具有至少90％同一性；与SEQ ID NO:109具有至少91％同一性；或与SEQ ID NO:83具有至少93％同一性；或与SEQ IDNO:91或103具有至少94％同一性；或与SEQ ID NO:95、101具有至少95％同一性；或与SEQID NO:99具有至少98％同一性。还设想了防治鳞翅目害虫的方法，其包括使所述害虫暴露于这种转基因植物细胞、植物或植物部分，其中所述植物细胞、植物或植物部分表达鳞翅目抑制量的所述嵌合杀昆虫蛋白质。

在本文中的其他实施方案中，提供了来源于所述植物细胞、植物或植物部分的商品产品，其中所述产品包含可检测量的所述嵌合杀昆虫蛋白质。所设想的商品产品包括植物生物质、油、膳食、动物饲料、面粉、薄片、糠、棉绒、外壳和经过处理的种子。

本文中所公开的又另一种方法是一种生产包含嵌合杀昆虫蛋白质的种子的方法，所述方法包括：种植至少一个包含嵌合杀昆虫蛋白质的种子；从所述种子长出植物；和从所述植物收获种子，其中所述收获的种子包含所述嵌合杀昆虫蛋白质。

本文中还设想了编码嵌合杀昆虫蛋白质的重组多核苷酸分子，其包含选自由以下各项组成的群组的核苷酸序列：1、2、3、5、6、8、9、11、12、14、15、17、18、20、22、24、26、27、29、31、32、34、35、37、38、40、42、44、46、48、49、51、52、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128、129或130；和任选地，编码与所述嵌合杀昆虫蛋白质不同的昆虫抑制剂的多核苷酸序列。

本文中所设想的另一种重组核酸分子包含可操作地连接至编码嵌合杀昆虫蛋白质的多核苷酸片段的异源启动子，其中：所述嵌合杀昆虫蛋白质包含如SEQ ID NO:21、10、28、7、4、13、16、19、23、25、30、33、36、39、41、43、45、47、50、53、55、57、59、61、63、65、67、69、71、73、75、77、79、81、83、85、87、89、91、93、95、97、99、101、103、105、107、109或111中的任意者中所示出的任意氨基酸序列；或所述嵌合杀昆虫蛋白质包含如下蛋白质：与SEQ ID NO:21、10具有至少94％同一性；与SEQ ID NO:28具有至少93％同一性；与SEQ ID NO:7具有至少87％同一性；与SEQ ID NO:4具有至少90％同一性；与SEQ ID NO:13具有至少91％同一性；与SEQ ID NO:16具有至少64％同一性；与SEQ IDNO：19具有至少66％同一性；与SEQ IDNO：23具有至少86％同一性；与SEQ ID NO：25具有至少91％同一性；与SEQ ID NO：30具有至少94％同一性；与SEQ ID NO：33具有至少91％同一性；与SEQ ID NO：36具有至少64％同一性；与SEQ ID NO：39具有至少66％同一性；与SEQ ID NO：41具有至少94％同一性；与SEQ IDNO：43具有至少84％同一性；与SEQ ID NO：45具有至少93％同一性；与SEQ ID NO：47具有至少94％同一性；与SEQ ID NO：50具有至少91％同一性；或与SEQ ID NO：53具有至少93％同一性；或与SEQ ID NO：85、93、105具有87％同一性；或与SEQ ID NO：55、57、59、61、63、65、67、69、71、73、75、77、79具有至少85％同一性；或与SEQ ID NO：91、87、89具有至少88％同一性；或与SEQ ID NO：107、111具有至少89％同一性；或与SEQ ID NO：97具有至少90％同一性；或与SEQ ID NO：109具有至少91％同一性；或与SEQ ID NO：83具有至少93％同一性；或与SEQ ID NO：91、103具有至少94％同一性；或与SEQ ID NO：95、101具有至少95％同一性；或与SEQ ID NO：99具有至少98％同一性；或者所述多核苷酸片段与具有如SEQ ID NO：1、2、3、5、6、8、9、11、12、14、15、17、18、20、22、24、26、27、29、31、32、34、35、37、38、40、42、44、46、48、49、51、52、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128、129或130中的任意者中所示出的核苷酸序列的多核苷酸杂交。

本发明的其他实施方案、特征和优点将从以下详细描述、实施例和权利要求书中显而易见。

序列简述

SEQ ID NO：1是用于在细菌细胞中表达的编码TIC1100的重组DNA序列。

SEQ ID NO:2是用于在植物细胞中表达的编码TIC1100的合成DNA序列。

SEQ ID NO:3是用于在植物细胞中表达的编码TIC1100的合成DNA序列。

SEQ ID NO:4是TIC1100的氨基酸序列。

SEQ ID NO:5是用于在细菌细胞中表达的编码TIC860的重组DNA序列。

SEQ ID NO:6是用于在植物细胞中表达的编码TIC860的合成DNA序列。

SEQ ID NO:7是TIC860的氨基酸序列。

SEQ ID NO:8是用于在细菌细胞中表达的编码TIC867的重组DNA序列。

SEQ ID NO:9是用于在植物细胞中表达的编码TIC867的合成DNA序列。

SEQ ID NO:10是TIC867的氨基酸序列。

SEQ ID NO:11是用于在细菌细胞中表达的编码TIC867_20的重组DNA序列。

SEQ ID NO:12是用于在植物细胞中表达的编码TIC867_20的合成DNA序列。

SEQ ID NO:13是TIC867_20的氨基酸序列。

SEQ ID NO:14是用于在细菌细胞中表达的编码TIC867_21的重组DNA序列。

SEQ ID NO:15是用于在植物细胞中表达的编码TIC867_21的合成DNA序列。

SEQ ID NO:16是TIC867_21的氨基酸序列。

SEQ ID NO:17是用于在细菌细胞中表达的编码TIC867_22的重组DNA序列。

SEQ ID NO:18是用于在植物细胞中表达的编码TIC867_22的合成DNA序列。

SEQ ID NO:19是TIC867_22的氨基酸序列。

SEQ ID NO:20是用于在植物细胞中表达的编码TIC867_23的合成DNA序列。

SEQ ID NO:21是TIC867_23的氨基酸序列。

SEQ ID NO:22是用于在植物细胞中表达的编码TIC867_24的合成DNA序列。

SEQ ID NO:23是TIC867_24的氨基酸序列。

SEQ ID NO:24是用于在植物细胞中表达的编码TIC867_24的合成DNA序列。

SEQ ID NO:25是TIC867_25的氨基酸序列。

SEQ ID NO:26是用于在细菌细胞中表达的编码TIC868的重组DNA序列。

SEQ ID NO:27是用于在植物细胞中表达的编码TIC868的合成DNA序列。

SEQ ID NO:28是TIC868的氨基酸序列。

SEQ ID NO:29是用于在植物细胞中表达的编码TIC868_9的合成DNA序列。

SEQ ID NO:30是TIC868_9的氨基酸序列。

SEQ ID NO:31是用于在细菌细胞中表达的编码TIC868_10的重组DNA序列。

SEQ ID NO:32是用于在植物细胞中表达的编码TIC868变异体TIC868_10的合成DNA序列。

SEQ ID NO:33是TIC868_10的氨基酸序列。

SEQ ID NO:34是用于在细菌细胞中表达的编码TIC868_11的重组DNA序列。

SEQ ID NO:35是用于在植物细胞中表达的编码TIC868_11的合成DNA序列。

SEQ ID NO:36是TIC868_11的氨基酸序列。

SEQ ID NO:37是用于在细菌细胞中表达的编码TIC868_12的重组DNA序列。

SEQ ID NO:38是用于在植物细胞中表达的编码TIC868_12的合成DNA序列。

SEQ ID NO:39是TIC868_12的氨基酸序列。

SEQ ID NO:40是用于在植物细胞中表达的编码TIC868_13的合成DNA序列。

SEQ ID NO:41是TIC868_13的氨基酸序列。

SEQ ID NO:42是用于在植物细胞中表达的编码TIC868_14的合成DNA序列。

SEQ ID NO:43是TIC868_14的氨基酸序列。

SEQ ID NO:44是用于在植物细胞中表达的编码TIC868_15的合成DNA序列。

SEQ ID NO:45是TIC868_15的氨基酸序列。

SEQ ID NO:46是用于在植物细胞中表达的编码TIC868_29的合成DNA序列。

SEQ ID NO:47是TIC868_29的氨基酸序列。

SEQ ID NO:48是用于在细菌细胞中表达的编码TIC869的重组DNA序列。

SEQ ID NO:49是用于在植物细胞中表达的编码TIC869的合成DNA序列。

SEQ ID NO:50是TIC869的氨基酸序列。

SEQ ID NO:51是用于在细菌细胞中表达的编码TIC836的重组DNA序列。

SEQ ID NO:52是用于在植物细胞中表达的编码TIC836的合成DNA序列。

SEQ ID NO:53是TIC836的氨基酸序列。

SEQ ID NO:54是编码嵌合TIC713氨基酸序列的DNA序列。

SEQ ID NO:55是从SEQ ID NO:54中所示出的开放阅读框翻译而来的TIC713氨基酸序列。

SEQ ID NO:56是编码嵌合TIC843氨基酸序列的DNA序列。

SEQ ID NO:57是从SEQ ID NO:56中所示出的开放阅读框翻译而来的TIC843氨基酸序列。

SEQ ID NO:58是编码嵌合TIC862氨基酸序列的DNA序列。

SEQ ID NO:59是从SEQ ID NO:58中所示出的开放阅读框翻译而来的TIC862氨基酸序列。

SEQ ID NO:60是编码嵌合TIC1099氨基酸序列的DNA序列。

SEQ ID NO:61是从SEQ ID NO:60中所示出的开放阅读框翻译而来的TIC1099氨基酸序列。

SEQ ID NO:62是编码嵌合TIC1099-T507E氨基酸序列的DNA序列。

SEQ ID NO:63是从SEQ ID NO:62中所示出的开放阅读框翻译而来的TIC1099-T507E氨基酸序列。

SEQ ID NO:64是编码嵌合TIC1099-R522K氨基酸序列的DNA序列。

SEQ ID NO:65是从SEQ ID NO:64中所示出的开放阅读框翻译而来的TIC1099-R522K氨基酸序列。

SEQ ID NO:66是编码嵌合TIC1099-K490S氨基酸序列的DNA序列。

SEQ ID NO:67是从SEQ ID NO:66中所示出的开放阅读框翻译而来的TIC1099-K490S氨基酸序列。

SEQ ID NO:68是编码嵌合TIC1099-T562R氨基酸序列的DNA序列。

SEQ ID NO:69是从SEQ ID NO:68中所示出的开放阅读框翻译而来的TIC1099-T562R氨基酸序列。

SEQ ID NO:70是编码嵌合TIC1099-S553R氨基酸序列的DNA序列。

SEQ ID NO:71是从SEQ ID NO:70中所示出的开放阅读框翻译而来的TIC1099-S553R氨基酸序列。

SEQ ID NO:72是编码嵌合TIC1099-G498D氨基酸序列的DNA序列。

SEQ ID NO:73是从SEQ ID NO:72中所示出的开放阅读框翻译而来的TIC1099-G498D氨基酸序列。

SEQ ID NO:74是编码嵌合TIC1099-K490A氨基酸序列的DNA序列。

SEQ ID NO:75是从SEQ ID NO:74中所示出的开放阅读框翻译而来的TIC1099-K490A氨基酸序列。

SEQ ID NO:76是编码嵌合TIC1099-E564A氨基酸序列的DNA序列。

SEQ ID NO:77是从SEQ ID NO:76中所示出的开放阅读框翻译而来的TIC1099-E564A氨基酸序列。

SEQ ID NO:78是编码嵌合TIC1103氨基酸序列的DNA序列。

SEQ ID NO:79是从SEQ ID NO:78中所示出的开放阅读框翻译而来的TIC1103氨基酸序列。

SEQ ID NO:80是编码嵌合TIC1101氨基酸序列的DNA序列。

SEQ ID NO:81是从SEQ ID NO:80中所示出的开放阅读框翻译而来的TIC1101氨基酸序列。

SEQ ID NO:82是编码嵌合TIC845氨基酸序列的DNA序列。

SEQ ID NO:83是从SEQ ID NO:82中所示出的开放阅读框翻译而来的TIC845氨基酸序列。

SEQ ID NO:84是编码嵌合TIC846氨基酸序列的DNA序列。

SEQ ID NO:85是从SEQ ID NO:84中所示出的开放阅读框翻译而来的TIC846氨基酸序列。

SEQ ID NO:86是编码嵌合TIC858氨基酸序列的DNA序列。

SEQ ID NO:87是从SEQ ID NO:86中所示出的开放阅读框翻译而来的TIC858氨基酸序列。

SEQ ID NO:88是编码嵌合TIC865氨基酸序列的DNA序列。

SEQ ID NO:89是从SEQ ID NO:88中所示出的开放阅读框翻译而来的TIC865氨基酸序列。

SEQ ID NO:90是编码嵌合TIC866氨基酸序列的DNA序列。

SEQ ID NO:91是从SEQ ID NO:90中所示出的开放阅读框翻译而来的TIC866氨基酸序列。

SEQ ID NO:92是编码嵌合TIC838氨基酸序列的DNA序列。

SEQ ID NO:93是从SEQ ID NO:92中所示出的开放阅读框翻译而来的TIC838氨基酸序列。

SEQ ID NO:94是编码嵌合TIC839氨基酸序列的DNA序列。

SEQ ID NO:95是从SEQ ID NO:94中所示出的开放阅读框翻译而来的TIC839氨基酸序列。

SEQ ID NO:96是编码嵌合TIC841氨基酸序列的DNA序列。

SEQ ID NO:97是从SEQ ID NO:96中所示出的开放阅读框翻译而来的TIC841氨基酸序列。

SEQ ID NO:98是编码嵌合TIC842氨基酸序列的DNA序列。

SEQ ID NO:99是从SEQ ID NO:98中所示出的开放阅读框翻译而来的TIC842氨基酸序列。

SEQ ID NO:100是编码嵌合TIC850氨基酸序列的DNA序列。

SEQ ID NO:101是从SEQ ID NO:100中所示出的开放阅读框翻译而来的TIC850氨基酸序列。

SEQ ID NO:102是编码嵌合TIC859氨基酸序列的DNA序列。

SEQ ID NO:103是从SEQ ID NO:102中所示出的开放阅读框翻译而来的TIC859氨基酸序列。

SEQ ID NO:104是编码嵌合TIC861氨基酸序列的DNA序列。

SEQ ID NO:105是从SEQ ID NO:104中所示出的开放阅读框翻译而来的TIC861氨基酸序列。

SEQ ID NO:106是编码嵌合TIC848氨基酸序列的DNA序列。

SEQ ID NO:107是从SEQ ID NO:106中所示出的开放阅读框翻译而来的TIC848氨基酸序列。

SEQ ID NO:108是编码嵌合TIC849氨基酸序列的DNA序列。

SEQ ID NO:109是从SEQ ID NO:108中所示出的开放阅读框翻译而来的TIC849氨基酸序列。

SEQ ID NO:110是编码嵌合TIC847氨基酸序列的DNA序列。

SEQ ID NO:111是从SEQ ID NO:110中所示出的开放阅读框翻译而来的TIC847氨基酸序列。

SEQ ID NO:112是用于在植物细胞中表达的编码TIC713的合成DNA序列。

SEQ ID NO:113是用于在植物细胞中表达的编码TIC713的合成DNA序列。

SEQ ID NO:114是用于在植物细胞中表达的编码TIC843的合成DNA序列。

SEQ ID NO:115是用于在植物细胞中表达的编码TIC862的合成DNA序列。

SEQ ID NO:116是用于在植物细胞中表达的编码TIC1099的合成DNA序列。

SEQ ID NO:117是用于在植物细胞中表达的编码TIC1103的合成DNA序列。

SEQ ID NO:118是用于在植物细胞中表达的编码TIC845的合成DNA序列。

SEQ ID NO:119是用于在植物细胞中表达的编码TIC846的合成DNA序列。

SEQ ID NO:120是用于在植物细胞中表达的编码TIC858的合成DNA序列。

SEQ ID NO:121是用于在植物细胞中表达的编码TIC866的合成DNA序列。

SEQ ID NO:122是用于在植物细胞中表达的编码TIC838的合成DNA序列。

SEQ ID NO:123是用于在植物细胞中表达的编码TIC841的合成DNA序列。

SEQ ID NO:124是用于在植物细胞中表达的编码TIC842的合成DNA序列。

SEQ ID NO:125是用于在植物细胞中表达的编码TIC850的合成DNA序列。

SEQ ID NO:126是用于在植物细胞中表达的编码TIC859的合成DNA序列。

SEQ ID NO：127是用于在植物细胞中表达的编码TIC861的合成DNA序列。

SEQ ID NO：128是用于在植物细胞中表达的编码TIC848的合成DNA序列。

SEQ ID NO：129是用于在植物细胞中表达的编码TIC849的合成DNA序列。

SEQ ID NO：130是用于在植物细胞中表达的编码TIC847的合成DNA序列。

发明详述

农业害虫防治领域中的问题的特征是需要对目标害虫有效果、对目标害虫物种表现出广谱毒性、能够在植物中表达而不会引起不希望的农艺学问题并且与市面上用于植物的当前毒素相比可提供替代作用模式的新杀昆虫蛋白质。本文中公开了新型嵌合杀昆虫蛋白质，并且解决了这些需要中的每一个，特别是对抗较宽范围的鳞翅目昆虫害虫。

为了避免发展或规避昆虫对目前使用的杀昆虫蛋白质的抗性，需要具有不同的作用模式(MOA)以及广谱性和功效的新杀昆虫蛋白质用于鳞翅目防治。解决这种需要的一种方式是从不同的生物学来源，优选地从细菌、真菌或植物中发现新的杀昆虫蛋白质。另一种方法是在表现出结构相似性的各种Bt蛋白质之间互换片段以产生具有昆虫抑制性质的新嵌合Bt蛋白质。本领域中已知由对本领域中已知的众多天然杀昆虫晶体蛋白质的结构域结构进行再拣选来产生具有增强的嵌合蛋白质的可能性微乎其微。参见例如JacquelineS.Knight等人，“A Strategy for Shuffling Numerous Bacillus thuringiensisCrystal Protein Domains.”J.Economic Entomology，97(6)(2004)：1805-1813。

本文中公开了编码新型嵌合杀昆虫蛋白质的重组核酸分子序列。这些杀昆虫蛋白质解决了本领域中持续需要工程改造其他毒性杀昆虫蛋白质以具有改善的杀昆虫性质(诸如对较宽范围的目标昆虫害虫物种的功效有所增加)和不同的作用模式。这组蛋白质的成员，包括本文中所公开的示例性蛋白质，对鳞翅目昆虫害虫物种表现出杀昆虫活性。

术语“片段(segment/fragment)”在本申请中用于描述比描述所公开的嵌合杀昆虫蛋白质的完整氨基酸或核酸序列短的连续氨基酸或核酸序列。本申请中还公开了表现出昆虫抑制活性的片段，如果将所述片段与所述嵌合杀昆虫蛋白质的相应部分比对，那么将得到在所述片段与所述嵌合杀昆虫蛋白质的相应部分之间存在从约65％至约100％的任何分数百分比的氨基酸序列同一性。

在本申请中提及术语“活性的”或“活性”、“杀虫活性”或“杀虫的”，或者“杀昆虫活性”、“昆虫抑制性”或“杀昆虫的”是指毒性剂，诸如杀昆虫蛋白质，在抑制(抑制生长、摄食、繁殖力或存活力)、遏制(遏制生长、摄食、繁殖力或存活力)、控制(控制害虫侵扰、控制含有有效量的杀昆虫蛋白质的特定作物上的害虫摄食活动)或杀死(引起发病、死亡或降低繁殖力)害虫方面的功效。这些术语意图包括对害虫提供杀虫有效量的杀昆虫蛋白质的结果，其中使所述害虫暴露于所述杀昆虫蛋白质将导致发病、死亡、繁殖力降低或发育迟缓。这些术语还包括由于在植物中或在植物上提供杀虫有效量的杀昆虫蛋白质而将害虫从植物、植物组织、植物部分、种子、植物细胞或从植物可能生长的特定地理位置中驱离。一般来说，杀虫活性是指杀昆虫蛋白质在抑制特定目标害虫(包括但不限于鳞翅目昆虫)的生长、发育、存活力、摄食行为、交配行为、繁殖力方面有效或对由以这种蛋白质、蛋白质片段、蛋白质片段或多核苷酸为食的昆虫引起的不利作用产生任何可测量的减少的能力。所述杀昆虫蛋白质可以由植物产生，或可以被施用至所述植物或施用至所述植物所处位置内的环境。术语“生物活性”、“有效的”、“有效果的”或其变化形式也是本申请中可互换用于描述本发明的嵌合杀昆虫蛋白质对目标昆虫害虫的作用的术语。

当提供于目标害虫的食物中时，杀虫有效量的毒性剂在所述毒性剂接触所述害虫时表现出杀虫活性。毒性剂可以是杀昆虫蛋白质或本领域中已知的一种或多种化学试剂。杀昆虫化学试剂和杀昆虫蛋白质试剂可以单独使用或与彼此组合使用。化学试剂包括但不限于靶向目标害虫中的特定基因以便遏制的dsRNA分子、有机氯化物、有机磷酸酯、氨基甲酸酯、拟除虫菊酯、新烟碱和莱恩碱。杀昆虫蛋白质试剂包括本申请中示出的嵌合杀昆虫蛋白质，以及其他蛋白质毒性剂，包括靶向鳞翅目害虫物种的那些，以及用于防治其他植物害虫的蛋白质毒素，诸如本领域中可用于防治鞘翅目、缨翅目、半翅目和同翅目物种的Cry蛋白质。

意图提及害虫、特别是作物植物的害虫意指作物植物的昆虫害虫，特别是通过所公开的嵌合杀昆虫蛋白质加以防治的那些鳞翅目昆虫害虫。然而，当靶向这些害虫的毒性剂与所述嵌合杀昆虫蛋白质或同所述嵌合杀昆虫蛋白质具有65％至约100％同一性的蛋白质共处或共存时，提及害虫还可以包括植物的鞘翅目、半翅目和同翅目昆虫害虫以及线虫和真菌。

本文中所公开的嵌合杀昆虫蛋白质对鳞翅目昆虫物种的昆虫害虫(包括成虫、蛹、幼虫和初孵幼虫)以及半翅目昆虫物种(包括成虫和若虫)表现出杀昆虫活性。鳞翅目昆虫包括但不限于夜蛾科的粘虫、切根虫、尺蠖和夜蛾，例如草地粘虫(草地贪夜蛾)、甜菜粘虫(甜菜夜蛾)、披肩粘虫(蓓带夜蛾)、黑切根虫(球菜夜蛾)、甘蓝尺蠖(粉纹夜蛾)、大豆尺蠖(大豆夜蛾)、黎豆毛虫(黎豆夜蛾)、绿啃叶虫(苜蓿绿夜蛾)、烟草芽虫(烟芽夜蛾)、颗粒切根虫(黄地老虎)、粘虫(一星粘虫)、西方切根虫(灰地老虎)；螟蛾科的钻心虫、鞘蛾、结网虫、球果虫、甘蓝虫和雕叶虫，例如，欧洲玉米钻心虫(欧洲玉米螟)、脐橙虫(脐橙螟)、玉米根结网虫(玉米根网螟)、草地结网虫(水稻切叶野螟)、向日葵蛾(向日葵螟)、小玉米茎钻心虫(南美玉米苗斑螟)；卷蛾科的卷叶虫、芽虫、种虫和果虫，例如，苹果蛾(苹果蠹蛾)、葡萄果实蛾(葡萄小食心虫)、东方果蛾(梨小食心虫)、向日葵芽小卷蛾(向日葵芽卷叶蛾)；和许多其他经济上重要的鳞翅目昆虫，例如钻背蛾(小菜蛾)、粉红棉铃虫(棉红铃虫)和吉普赛蛾(舞毒蛾)。其他鳞翅目昆虫害虫包括例如棉叶波纹夜蛾(棉叶虫)、果树黄卷蛾(果树卷叶虫)、玫瑰黄卷蛾(欧洲卷叶虫)和其他黄卷蛾属、二化螟(亚洲水稻钻心虫或水稻钻茎虫)、稻纵卷叶螟(水稻卷叶虫)、玉米根网螟(玉米根结网虫)、早熟禾草暝(蓝草结网虫)、西南玉米螟(西南玉米钻心虫)、小蔗螟(蔗螟)、埃及金刚钻(多刺棉铃虫)、翠纹金刚钻(斑点棉铃虫)、棉铃实夜蛾(美洲棉铃虫)、玉米穗夜蛾(玉米穗虫或棉铃虫)、烟芽夜蛾(烟草芽虫)、水稻切叶野螟(草地结网虫)、欧洲葡萄缀穗蛾(欧洲葡萄蛾)、柑桔潜叶蛾(柑桔潜叶虫)、大菜粉蛾(大白蝴蝶)、小菜粉蛾(菜青虫或小白蝴蝶)、小菜蛾(钻背蛾)、甜菜夜蛾(甜菜粘虫)、斜纹夜蛾(烟草切根虫、茶蚕)和番茄潜叶蛾(番茄潜叶虫)。

本申请中提及“分离的DNA分子”或者等效术语或短语意图意指所述DNA分子是单独或与其他组合物组合存在但不在其天然环境内的DNA分子。举例来说，只要生物体基因组DNA内天然存在的核酸元件，诸如编码序列、内含子序列、非翻译前导序列、启动子序列、转录终止序列等在所述生物体基因组内且在天然存在其的基因组内位置上，所述元件就不被认为是“分离的”。然而，只要这些元件中的每一个和这些元件的子部分不是在所述生物体基因组内且在天然存在其的基因组内位置上，所述元件在本公开的范围内就将是“分离的”。类似地，只要编码杀昆虫蛋白质或所述蛋白质的任何天然存在的杀昆虫变体的核苷酸序列不在从中天然存在编码所述蛋白质的序列的细菌DNA内，所述核苷酸序列就将是分离的核苷酸序列。出于本公开的目的，编码天然存在的杀昆虫蛋白质的氨基酸序列的合成核苷酸序列将被认为是分离的。出于本公开的目的，任何转基因核苷酸序列，即插入植物或细菌细胞基因组中或存在于染色体外载体中的DNA核苷酸序列将被认为是分离的核苷酸序列，无论其存在于质粒内或用于转化所述细胞的类似结构内、所述植物或细菌的基因组内，还是以可检测的量存在于来源于所述植物或细菌的组织、子代、生物样品或商品产品中。

如实施例中进一步描述，通过嵌合工作，由已知杀昆虫毒素(在本文中被称为“亲本蛋白质”的原毒素和毒素结构域构建了约八百四十四(844)个编码嵌合杀昆虫蛋白质的核苷酸序列，并且加以表达且在生物测定中测试鳞翅目活性。与从中得到其毒素组分的亲本蛋白质相比，所构建的嵌合杀昆虫蛋白质中少数表现出提高的鳞翅目活性或增大的鳞翅目谱。

这些具有提高的鳞翅目活性或增大的鳞翅目谱的新型嵌合杀昆虫蛋白质是由以下杀昆虫亲本蛋白质原毒素和毒素结构域构建：Cry1Ah(结构域I)、Cry1Bb1(结构域I和II)、Cry1Be2(结构域I和II)、Cry1Ja1(结构域I和II)、Cry1Fa1(结构域I和II)、Cry1Ac(结构域II和结构域原毒素)、Cry1Ca(结构域III和结构域原毒素)、Cry1Ka(结构域III和结构域原毒素)、Cry1Jx(结构域III)、Cry1Ab(结构域III)、Cry1Ab3(原毒素)、Cry1Da1(原毒素)、Cry4(原毒素)、Cry9(原毒素)、Cry1Be(原毒素),和结构域Cry1Ka(原毒素)。

具体来说，本发明的具有提高的鳞翅目活性或增大的鳞翅目谱的新型嵌合杀昆虫蛋白质包含以下原毒素与结构域组合：TIC1100/SEQ ID NO:4(结构域I-Cry1Ah、结构域II-Cry1Ac、结构域III-Cry1Ca、原毒素-Cry1Ac)、TIC860/SEQ ID NO:7(结构域I-Cry1Bb1、结构域II-Cry1BB1、结构域III-Cry1Ca、原毒素-Cry1Ac)、TIC867/SEQ ID NO:10(结构域I-Cry1Be2、结构域II-Cry1Be2、结构域III-Cry1Ka、原毒素-Cry1Ab3)、TIC868/SEQ ID NO:28(结构域I-Cry1Be2、结构域II-Cry1Be2和结构域III-Cry1Ca、原毒素-Cry1Ab3)、TIC869/SEQ ID NO:50(结构域I-Cry1Ja1、结构域II-Cry1Ja1、结构域III-Cry1Jx、原毒素-Cry1Ab3)和TIC836/SEQ ID NO:53(结构域I-Cry1Fa1、结构域II-Cry1Fa1、结构域III-Cry1Ab、原毒素-Cry1Ac)。

对于嵌合杀昆虫蛋白质TIC867和TIC868，还构建了引入氨基酸取代或替代原毒素结构域的变体。具体来说，TIC867和TIC868的这些变体包含以下氨基酸取代或替代原毒素结构域：TIC867_20/SEQ ID NO:13(替代原毒素结构域Cry1Da1)、TIC867_21/SEQ ID NO:16(替代原毒素结构域Cry4)、TIC867_22/SEQ ID NO:19(替代原毒素结构域Cry9)、TIC867_23/SEQ ID NO:21(替代原毒素结构域Cry1Be)、TIC867_24/SEQ ID NO:23(替代原毒素结构域Cry1Ka)、TIC867_25/SEQ ID NO:25(替代原毒素结构域Cry1Ka)、TIC868_9/SEQ ID NO:30(氨基酸修饰N240S_Y343Q_N349T)、TIC868_10/SEQ ID NO:33(替代原毒素结构域Cry1Da1)、TIC868_11/SEQ ID NO:36(替代原毒素结构域Cry4)、TIC868_12/SEQ ID NO:39(替代原毒素结构域Cry 9),TIC868_13/SEQ ID NO:41(替代原毒素结构域Cry1Be)、TIC868_14/SEQ ID NO:43(替代原毒素结构域Cry1Ka)、TIC868_15/SEQ ID NO:45(替代原毒素结构域Cry1Ca)和TIC868_29/SEQ ID NO:47(氨基酸修饰Q136Y_Y343Q_N349T)。

如实施例中所显示，这些TIC867和TIC868变体中的每一种都能改变亲本嵌合杀昆虫蛋白质的鳞翅目活性和/或减小鳞翅目活性谱，因此表明替代原毒素结构域和氨基酸取代对嵌合杀昆虫蛋白质TIC867和TIC868的杀昆虫活性和谱具有直接后果。

所述嵌合杀昆虫蛋白质中有许多种对多个鳞翅目昆虫害虫物种显示了杀昆虫活性。具体来说，本申请中所公开的新型嵌合杀昆虫蛋白质对一种或多种以下鳞翅目昆虫害虫表现出活性：黎豆毛虫(VBC，黎豆夜蛾)、蔗螟(SCB，小蔗螟)、小玉米茎钻心虫(LSCB，南美玉米苗斑螟)、玉米穗虫(CEW，玉米穗夜蛾)、大豆荚虫(SPW，玉米穗夜蛾)、棉铃虫(CBW，玉米穗夜蛾)、烟草芽虫(TBW，烟芽夜蛾)、大豆尺蠖(SBL，大豆夜蛾)、黑粘虫(BLAW，考斯夜蛾)、南方粘虫(SAW，亚热带粘虫)、草地粘虫(FAW，草地贪夜蛾)、甜菜粘虫(BAW，甜菜夜蛾)、古棉铃虫(OBW，棉铃实夜蛾)、东方叶虫(OLW，斜纹夜蛾)、粉红棉铃虫(PBW，棉红铃虫)、西南玉米钻心虫(SWCB，西南玉米螟)、斑点棉铃虫(SBW，翠纹金刚钻)、美洲棉铃虫(SABW，南美棉铃虫)和向日葵尺蠖(SFL，薄荷灰夜蛾)。因此，本申请中所描述的示例性蛋白质因常见功能而相关并且对鳞翅目昆虫物种的昆虫害虫，包括成虫、幼虫和蛹，表现出杀昆虫活性。

与所述嵌合杀昆虫蛋白质类似的蛋白质可以通过使用本领域中已知的基于计算机的算法与彼此比较来加以鉴别。举例来说，可以使用Clustal W比对，使用以下这些默认参数来分析蛋白质相对于嵌合杀昆虫蛋白质的氨基酸序列同一性：权重矩阵：blosum，空位开放罚分：10.0，空位延伸罚分：0.05，亲水性空位：On，亲水性残基：GPSNDQERK，残基特异性空位罚分：On(Thompson等人,(1994)Nucleic Acids Research,22:4673-4680)。通过100％×(氨基酸同一性/标的蛋白质的长度)的乘积来进一步计算氨基酸同一性百分比。还可利用本领域中的其他比对算法，得到与使用Clustal W比对获得的结果类似的结果，且涵盖在本申请中。

意图本申请中公开一种表现出昆虫抑制活性的查询蛋白质，在比对此类查询蛋白质与SEQ ID NO:4、7、10、13、16、19、21、23、25、28、30、33、36、39、41、43、45、47、50、53、55、57、59、61、63、65、67、69、71、73、75、77、79、81、83、85、87、89、91、93、95、97、99、101、103、105、107、109和111中所示出的标的嵌合杀昆虫蛋白质时，在所述查询蛋白质与所述标的蛋白质之间获得至少约64％、65％、66％、67％、68％、69％、70％、71％、72％、73％、74％、75％、76％、77％、78％、79％、80％、81％、82％、83％、84％、85％、86％、87％、88％、89％、90％、91％、92％、93％、94％、95％、96％、97％、98％、99％或约100％氨基酸序列同一性(或此范围中的任何分数百分比)。

如本申请的实施例中进一步描述，设计编码所述嵌合杀昆虫蛋白质的合成序列或人工序列以供用于植物中。设计用于植物中的示例性合成核苷酸序列示出于SEQ ID NO:2和3(TIC1100)、SEQ ID NO:6(TIC860)、SEQ ID NO:9(TIC867)、SEQ ID NO:12(TIC867_20)、SEQ ID NO:15(TIC867_21)、SEQ ID NO:18(TIC867_22)、SEQ ID NO:20(TIC867_23)、SEQID NO:22(TIC867_24)、SEQ ID NO:24(TIC867_25)、SEQ ID NO:27(TIC868)、SEQ ID NO:29(TIC868_9)、SEQ ID NO:32(TIC868_10)、SEQ ID NO:35(TIC868_11)、SEQ ID NO:38(TIC868_12)、SEQ ID NO:40(TIC868_13)、SEQ ID NO:42(TIC868_14)、SEQ ID NO:44(TIC868_15)、SEQ ID NO:46(TIC868_29)、SEQ ID NO:49(TIC869)和SEQ ID NO:52(TIC836)、SEQ ID NO:112和113(TIC713)、SEQ ID NO:114(TIC843)、SEQ ID NO:115(TIC862)、SEQ ID NO:116(TIC1099)、SEQ ID NO:117(TIC1103)、SEQ ID NO:118(TIC845)、SEQ ID NO:119(TIC846)、SEQ ID NO:120(TIC858)、SEQ ID NO:121(TIC866)、SEQ ID NO:122(TIC838)、SEQ ID NO:123(TIC841)、SEQ ID NO:124(TIC842)、SEQ ID NO:125(TIC850)、SEQ ID NO:126(TIC859)、SEQ ID NO:127(TIC861)、SEQ ID NO:128(TIC848)、SEQ ID NO:129(TIC849)和SEQ ID NO:130(TIC847)中。

为了在植物细胞中表达，所述嵌合杀昆虫蛋白质可以被表达以存在于细胞溶质中或靶向植物细胞的各种细胞器。举例来说，使蛋白质靶向叶绿体可能导致转基因植物中的表达蛋白质的水平增加，同时防止出现脱靶表型。靶向还可能导致转基因事件中的害虫抗性功效增加。靶标肽或转运肽是指导蛋白质转运至细胞中特定区域，包括细胞核、线粒体、内质网(ER)、叶绿体、质外体、过氧化物酶体和胞质膜的短(3至70个氨基酸长)肽链。在蛋白质被转运之后通过信号肽酶使一些靶标肽从所述蛋白质上裂解。为了靶向叶绿体，蛋白质含有大约40至50个氨基酸的转运肽。关于叶绿体转运肽的使用的描述，参见美国专利No.5,188,642和5,728,925。许多位于叶绿体的蛋白质是从细胞核基因表达为前体，并且通过叶绿体转运肽(CTP)靶向叶绿体。此类分离的叶绿体蛋白质的实例包括但不限于与核酮糖-1,5-双磷酸羧化酶、铁氧化还原蛋白、铁氧化还原蛋白氧化还原酶、捕光复合体蛋白I和蛋白II、硫氧化还原蛋白F、烯醇丙酮莽草酸磷酸合酶(EPSPS)和美国专利No.7,193,133中所描述的转运肽的小亚单元(SSU)缔合的那些。已经在活体内和活体外证明，可以通过使用与异源CTP的蛋白质融合物使非叶绿体蛋白质靶向叶绿体，而且CTP足以使蛋白质靶向叶绿体。并入合适的叶绿体转运肽，诸如阿拉伯芥EPSPS CTP(CTP2)(参见Klee等人,Mol.Gen.Genet.210:437-442,1987)或矮牵牛EPSPS CTP(CTP4)(参见della-Cioppa等人,Proc.Natl.Acad.Sci.USA 83:6873-6877,1986)已经证明异源EPSPS蛋白质序列靶向转基因植物中的叶绿体(参见美国专利No.5,627,061、5,633,435和5,312,910；以及EP0218571、EP 189707、EP 508909和EP 924299)。为了使嵌合杀昆虫蛋白质靶向叶绿体，相对于已经设计的用于在植物细胞中进行最佳表达的编码嵌合杀昆虫蛋白质的合成编码序列，将编码叶绿体转运肽的序列的放在5'处的可操作键联中和框架中。

根据本领域中已知的转化方法和技术来构建含有这些合成或人工核苷酸序列的表达盒和载体并且将其引入玉米、棉花和大豆植物细胞中。使转化细胞再生出转化植物，观察到所述转化植物表达所述嵌合杀昆虫蛋白质。为了测试杀虫活性，在存在鳞翅目害虫幼虫的情况下使用获自转化植物的植物叶盘进行生物测定。设想了编码所述嵌合杀昆虫蛋白质的重组核酸分子组合物。举例来说，可以用重组DNA构建体表达所述嵌合杀昆虫蛋白质，其中编码嵌合杀昆虫蛋白质的具有ORF的多核苷酸分子可操作地连接至诸如启动子之类的基因表达元件和在所述构建体所意图的系统中表达所必需的任何其他调控元件。非限制性实例包括与合成嵌合杀昆虫蛋白质编码序列可操作地连接以便在植物中表达所述嵌合杀昆虫蛋白质的植物功能启动子或与嵌合杀昆虫蛋白质编码序列可操作地连接以便在Bt细菌或其他芽孢杆菌属中表达所述蛋白质的Bt功能启动子。其他元件可以可操作地连接至所述嵌合杀昆虫蛋白质编码序列，包括但不限于增强子、内含子、未翻译前导序列、编码蛋白质固定标签(HIS标签)、转位肽(即，质粒转运肽、信号肽)、针对翻译后修饰酶的多肽序列、核糖体结合位点和RNAi标靶位点。

本文中所提供的示例性重组多核苷酸分子包括但不限于与编码具有如SEQ IDNO:4(TIC1100)、7(TIC860)、10(TIC867)、13(TIC867_20)、16(TIC867_21)、19(TIC867_22)、21(TIC867_23)、23(TIC867_24)、25(TIC867_25)、28(TIC868)、30(TIC868_9)、33(TIC868_10)、36(TIC868_11)、39(TIC867_12)、41(TIC867_13)、43(TIC867_14)、45(TIC867_15)、47(TIC867_29)、50(TIC869)、53(TIC836)、55(TIC713)、57(TIC843)、59(TIC862)、61(TIC1099)、63(TIC1099-T507E)、65(TIC1099-R522K)、67(TIC1099-K490S)、69(TIC1099-T562R)、71(TIC1099-S533R)、73(TIC1099-G498D)、75(TIC1099-K490A)、77(TIC1099-E564A)、79(TIC1103)、81(TIC1101)、83(TIC845)、85(TIC846)、87(TIC858)、89(TIC865)、91(TIC866)、93(TIC838)、95(TIC839)、97(TIC841)、99(TIC842)、101(TIC850)、103(TIC859)、105(TIC861)、107(TIC848)、109(TIC849)和111(TIC847)中所示出的氨基酸序列的多肽或蛋白质的诸如SEQ ID NO:1、2、3、5、6、8、9、11、12、14、15、17、18、20、22、24、26、27、29、31、32、34、35、37、38、40、42、44、46、48、49、51、52、54、56、58、60、62、64、66、68、70、72、74、76、78、80、82、84、86、88、90、92、94、96、98、100、102、104、106、108、110、112、113、114、115、116、117、118、119、120、121、122、123、124、125、126、127、128、129和130之类的多核苷酸可操作地连接的异源启动子。异源启动子还可以可操作地连接至编码质粒靶向的嵌合杀昆虫蛋白质和未靶向的嵌合杀昆虫蛋白质的合成DNA编码序列。设想编码本文中所公开的嵌合杀昆虫蛋白质的重组核酸分子的密码子可以被同义密码子取代(在本领域中被称为沉默取代)。

包含嵌合杀昆虫蛋白质编码序列的重组DNA分子或构建体可以进一步包含编码一种或多种毒性剂的DNA区域，所述DNA区域可以经配置以便与编码嵌合杀昆虫蛋白质、不同于嵌合杀昆虫蛋白质的蛋白质、昆虫抑制性dsRNA分子或辅助蛋白质的DNA序列伴随表达或共同表达。辅助蛋白质包括但不限于辅因子、酶、结合伴侣或功能在于辅助昆虫抑制剂的效果，例如通过辅助其表达、影响其在植物中的稳定性、优化寡聚自由能、加强其毒性和增加其活性谱的其他试剂。辅助蛋白质可以例如促进一种或多种昆虫抑制剂的吸收或增强毒性剂的毒性效果。

可以组装重组DNA分子或构建体，使得所有蛋白质或dsRNA分子都可以从一种启动子表达，或者每一种蛋白质或dsRNA分子都在单独启动子控制或其一些组合下。本发明的蛋白质可以由多基因表达系统表达，其中嵌合杀昆虫蛋白质由常见核苷酸片段表达，所述核苷酸片段还含有其他开放阅读框和启动子，取决于所选表达系统的类型。举例来说，细菌多基因表达系统可以利用单个启动子来驱动多个连接/串联开放阅读框从单个操纵子内表达(即，多顺反子表达)。在另一个实例中，植物多基因表达系统可以利用多个未连接的表达盒，每一个表达盒表达不同的蛋白质或其他毒性剂，诸如一或多个dsRNA分子。

可以通过载体，例如质粒、杆状病毒、合成染色体、病毒体、粘粒、噬菌粒、噬菌体或病毒载体，将包含嵌合杀昆虫蛋白质编码序列的重组核酸分子或重组DNA构建体递送至宿主细胞。此类载体可用于实现嵌合杀昆虫蛋白质编码序列在宿主细胞中的稳定或暂时表达或者所编码的多肽的后续表达。包含嵌合杀昆虫蛋白质序列编码序列并且被引入宿主细胞中的外源重组多核苷酸或重组DNA构建体在本文中被称为“转基因”。

本文中提供了含有编码所述嵌合杀昆虫蛋白质中的任何一种或多种的多核苷酸的转基因细菌、转基因植物细胞、转基因植物和转基因植物部分。术语“细菌细胞”或“细菌”可以包括但不限于土壤杆菌、芽孢杆菌、埃希氏杆菌、沙门氏菌、假单胞菌或根瘤菌细胞。术语“植物细胞”或“植物”可以包括但不限于双子叶细胞或单子叶细胞。所设想的植物和植物细胞包括但不限于苜蓿、香蕉、大麦、豆、茎椰菜、甘蓝、芸苔、胡萝卜、木薯、蓖麻、花椰菜、芹菜、鹰嘴豆、大白菜、柑桔、椰子、咖啡、玉米、三叶草、棉花、葫芦、黄瓜、花旗松、茄子、桉树、亚麻、大蒜、葡萄、啤酒花、韭菜、莴苣、火炬松、小米、甜瓜、坚果、燕麦、橄榄树、洋葱、观赏植物、棕榈、牧草、豌豆、花生、胡椒、木豆、松树、马铃薯、白杨树、南瓜、辐射松、萝卜、油菜籽、水稻、根茎、黑麦、红花、灌木、高粱、南方松、大豆、菠菜、南瓜、草莓、糖用甜菜、甘蔗、向日葵、甜玉米、香枫、甜薯、柳枝稷、茶树、烟草、番茄、黑小麦、草坪草、西瓜和小麦植物细胞或植物。在某些实施方案中，提供了由转基因植物细胞再生的转基因植物和转基因植物部分。在某些实施方案中，所述转基因植物可以获自转基因种子、通过插枝、折断、研磨或其他方式使部分从植物脱离。在某些实施方案中，所述植物部分可以是种子、蒴、叶、花、茎、根或其任何部分，或者转基因植物部分的不可再生部分。如这种情况下所使用，转基因植物部分的“不可再生”部分是不能受到诱导而形成完整植物或不能受到诱导而形成能够进行有性繁殖和/或无性繁殖的完整植物的部分。在某些实施方案中，植物部分的不可再生部分是转基因种子、蒴、叶、花、茎或根的一部分。

提供了制造包含鳞翅目抑制量的嵌合杀昆虫蛋白质的转基因植物的方法。此类植物可以通过将编码本申请中所提供的嵌合杀昆虫蛋白质的多核苷酸引入植物细胞中和选择表达昆虫或鳞翅目抑制量的嵌合杀昆虫蛋白质的来源于所述植物细胞的植物来制造。植物可以通过再生、种子、花粉或分生组织转化技术而来源于植物细胞。用于转化植物的方法在本领域中是已知的。举例来说，土壤杆菌介导的转化描述于美国专利申请公布2009/0138985A1(大豆)、2008/0280361A1(大豆)、2009/0142837A1(玉米)、2008/0282432(棉花)和2008/0256667(棉花)中。

表达所述嵌合杀昆虫蛋白质的植物可以通过利用表达其他杀昆虫蛋白质和/或表达其他转基因特质(诸如其他昆虫防治特质)的转基因事件、除草剂耐受基因、赋予产量或应力耐受特质的基因等等进行育种来进行杂交，或可以将此类特质组合在单个载体中，从而将所述特质全部联系起来。

本申请中还公开了经过加工的植物产品，其中所述经过加工的产品包含可检测量的嵌合杀昆虫蛋白质、其昆虫抑制性片段或其任何区别部分。在某些实施方案中，所述经过加工的产品是选自由以下各项组成的群组：植物部分、植物生物质、油、膳食、糖、动物饲料、面粉、薄片、糠、棉绒、外壳、经过加工的种子和种子。在某些实施方案中，所述经过加工的产品是不可再生的。所述植物产品可以包含来源于转基因植物或转基因植物部分的商品或其他商业产品，其中所述商品或其他产品可以通过检测编码或包含嵌合杀昆虫蛋白质的特色部分的核苷酸片段或者表达的RNA或蛋白质来通过商业进行追踪。

本申请中还公开了用所述嵌合杀昆虫蛋白质来防治昆虫、特别是鳞翅目昆虫侵扰作物植物的方法。此类方法可以包括种植包含昆虫或鳞翅目抑制量的所述嵌合杀昆虫蛋白质的植物。在某些实施方案中，此类方法可以进一步包括以下操作中的任何一个或多个：(i)向植物或能产生植物的种子施用包含或编码嵌合杀昆虫蛋白质的任何组合物；和(ii)用编码嵌合杀昆虫蛋白质的多核苷酸对植物或能产生植物的植物细胞进行转化。一般来说，设想嵌合杀昆虫蛋白质可以提供于组合物中，提供于微生物中，或提供于转基因植物中，以赋予针对鳞翅目昆虫的昆虫抑制活性。

在某些实施方案中，所述嵌合杀昆虫蛋白质是通过培养经过转化以便在适合表达的条件下表达嵌合杀昆虫蛋白质的重组芽孢杆菌或任何其他重组细菌细胞而制备的昆虫抑制性组合物的杀昆虫活性成分。此类组合物可以通过对表达/产生所述嵌合杀昆虫蛋白质的此类重组细胞的培养物进行干燥、冻干、均质化、提取、过滤、离心、沉降或浓缩来制备。此类方法可以获得芽孢杆菌或其他昆虫病原细菌细胞提取物、细胞悬浮液、细胞匀浆、细胞溶解产物、细胞上清液、细胞滤液或细胞球粒。通过获得如此产生的嵌合杀昆虫蛋白质，包括所述嵌合杀昆虫蛋白质的组合物可以包括细菌细胞、细菌孢子和伴孢包涵体，并且可以经过配制以用于各种用途，包括作为农业昆虫抑制性喷雾产品或作为食物生物测定中的昆虫抑制性制剂。

上述化合物或制剂可以进一步包含农业上可接受的载体，诸如诱饵、粉末、粉尘、球粒、颗粒、喷雾、乳液、胶态悬浮液、水溶液、牙胞杆菌孢子或晶体制剂或种子处理。所述化合物或制剂还可以进一步包含经转化以表达一种或多种所述蛋白质的重组植物细胞、植物组织、种子或植物；或经转化以表达一种或多种所述蛋白质的细菌。取决于所述重组多肽固有的昆虫抑制或杀昆虫抑制水平和应用于植物或食物测定的化合物或制剂水平，所述化合物或制剂可以包括各种以重量计量的所述重组多肽，例如以重量计0.0001％至0.001％至0.01％至1％至99％的所述重组多肽。

在一个实施方案中，为了降低抗性发展的可能性，包含嵌合杀昆虫蛋白质的昆虫抑制组合物或转基因植物可以进一步包含至少一种其他毒性剂，所述毒性剂对相同鳞翅目昆虫物种表现出昆虫抑制活性但不同于所述嵌合杀昆虫蛋白质。用于此类组合物的其他可能毒性剂包括昆虫抑制性蛋白质和昆虫抑制性dsRNA分子。使用此类核糖核苷酸序列来防治昆虫害虫的一个实例描述于Baum等人(美国专利公布2006/0021087A1)中。用于防治鳞翅目害虫的此类其他多肽可以选自由昆虫抑制性蛋白质组成的群组，诸如但不限于Cry1A(美国专利No.5,880,275)、Cry1Ab、Cry1Ac、Cry1A.105、Cry1Ae、Cry1B(美国专利公布No.10/525,318)、Cry1C(美国专利No.6,033,874)、Cry1D、Cry1E、Cry1F和Cry1A/F嵌合体(美国专利No.7,070,982、6,962,705和6,713,063)、Cry1G、Cry1H、Cry1I、Cry1J、Cry1K、Cry1L、Cry2A、Cry2Ab(美国专利No.7,064,249)、Cry2Ae、Cry4B、Cry6、Cry7、Cry8、Cry9、Cry15、Cry43A、Cry43B、Cry51Aa1、ET66、TIC400、TIC800、TIC834、TIC1415、Vip3A、VIP3Ab、VIP3B、AXMI-001、AXMI-002、AXMI-030、AXMI-035和AXMI-045(美国专利公布2013-0117884A1)、AXMI-52、AXMI-58、AXMI-88、AXMI-97、AXMI-102、AXMI-112、AXMI-117、AXMI-100(美国专利公布2013-0310543A1)、AXMI-115、AXMI-113、AXMI-005(美国专利公布2013-0104259A1)、AXMI-134(美国专利公布2013-0167264A1)、AXMI-150(美国专利公布2010-0160231A1)、AXMI-184(美国专利公布2010-0004176A1)、AXMI-196、AXMI-204、AXMI-207、AXMI-209(美国专利公布2011-0030096A1)、AXMI-218、AXMI-220(美国专利公布2014-0245491A1)、AXMI-221z、AXMI-222z、AXMI-223z、AXMI-224z、AXMI-225z(美国专利公布2014-0196175A1)、AXMI-238(美国专利公布2014-0033363A1)、AXMI-270(美国专利公布2014-0223598A1)、AXMI-345(美国专利公布2014-0373195A1)、DIG-3(美国专利公布2013-0219570A1)、DIG-5(美国专利公布2010-0317569A1)、DIG-11(美国专利公布2010-0319093A1)、AfIP-1A及其衍生物(美国专利公布2014-0033361A1)、AfIP-1B及其衍生物(美国专利公布2014-0033361A1)、PIP-1APIP-1B(美国专利公布2014-0007292A1)、PSEEN3174(美国专利公布2014-0007292A1)、AECFG-592740(美国专利公布2014-0007292A1)、Pput_1063(美国专利公布2014-0007292A1)、Pput_1064(美国专利公布2014-0007292A1)、GS-135及其衍生物(美国专利公布2012-0233726A1)、GS153及其衍生物(美国专利公布2012-0192310A1)、GS154及其衍生物(美国专利公布2012-0192310A1)、GS155及其衍生物(美国专利公布2012-0192310A1)、如美国专利公布2012-0167259A1中所描述的SEQ ID NO:2及其衍生物、如美国专利公布2012-0047606A1中所描述的SEQ ID NO:2及其衍生物、如美国专利公布2011-0154536A1中所描述的SEQ ID NO:2及其衍生物、如美国专利公布2011-0112013A1中所描述的SEQ ID NO:2及其衍生物、如美国专利公布2010-0192256A1中所描述的SEQ ID NO:2和4及其衍生物、如美国专利公布2010-0077507A1中所描述的SEQ ID NO:2及其衍生物、如美国专利公布2010-0077508A1中所描述的SEQ ID NO:2及其衍生物、如美国专利公布2009-0313721A1中所描述的SEQ ID NO:2及其衍生物、如美国专利公布2010-0269221A1中所描述的SEQ ID NO:2或4及其衍生物、如美国专利No.7,772,465(B2)中所描述的SEQ ID NO:2及其衍生物、如WO2014/008054A2中所描述的CF161_0085及其衍生物、如美国专利公布US2008-0172762A1、US2011-0055968A1和US2012-0117690A1中所描述的鳞翅目毒性蛋白质及其衍生物、如US7510878(B2)中所描述的SEQ ID NO:2及其衍生物、如美国专利No.7812129(B1)中所描述的SEQ ID NO:2及其衍生物；等等。

在其他实施方案中，昆虫抑制组合物或转基因植物可以进一步包含对不受本发明的嵌合杀昆虫蛋白质抑制的昆虫害虫(诸如鞘翅目、半翅目和同翅目害虫)表现出昆虫抑制活性的至少一种其他毒性剂，以便扩大所获得的昆虫抑制的范围。

用于防治鞘翅目害虫的此类其他毒性剂可以选自由昆虫抑制性蛋白质组成的群组，诸如但不限于Cry3Bb(美国专利No.6,501,009)、Cry1C变体、Cry3A变体、Cry3、Cry3B、Cry34/35、5307、AXMI134(美国专利公布2013-0167264A1)、AXMI-184(美国专利公布2010-0004176A1)、AXMI-205(美国专利公布2014-0298538A1)、axmi207(美国专利公布2013-0303440A1)、AXMI-218、AXMI-220(美国专利公布20140245491A1)、AXMI-221z、AXMI-223z(美国专利公布2014-0196175A1)、AXMI-279(美国专利公布2014-0223599A1)、AXMI-R1和其变体(美国专利公布2010-0197592A1)、TIC407、TIC417、TIC431、TIC807、TIC853、TIC901、TIC1201、TIC3131、DIG-10(美国专利公布2010-0319092A1)、eHIP(美国专利申请公布No.2010/0017914)、IP3和其变体(美国专利公布2012-0210462A1)和ω-Hexatoxin-Hv1a(美国专利申请公布US2014-0366227A1)。

用于防治半翅目害虫的此类其他毒性剂可以选自由半翅目活性蛋白质组成的群组，诸如但不限于TIC1415(美国专利公布2013-0097735A1)、TIC807(美国专利No.8609936)、TIC834(美国专利公布2013-0269060A1)、AXMI-036(美国专利公布2010-0137216A1)和AXMI-171(美国专利公布2013-0055469A1)。用于防治鞘翅目、鳞翅目和半翅目昆虫害虫的其他多肽可以在由Neil Crickmore维护的苏云金芽孢杆菌毒素命名网站(www.btnomenclature.info)上找到。

可以使用本领域技术人员已知的方法，诸如聚合酶链反应(PCR)、热扩增和杂交来鉴别嵌合杀昆虫蛋白质编码序列和与所述嵌合杀昆虫蛋白质具有相当大同一性百分比的序列。举例来说，所述嵌合杀昆虫蛋白质可用于产生特异性结合相关蛋白质的抗体，并且可用于筛选和发现密切相关的其他蛋白质。

此外，编码所述嵌合杀昆虫蛋白质的核苷酸序列可以用作筛选用探针和引物，以便使用热循环或等温扩增和杂交方法来鉴别所述类别的其他成员。举例来说，来源于如SEQID NO：2中所示出的序列的寡核苷酸可用于确定来源于商品产品的脱氧核糖核酸样品中存在或不存在嵌合杀昆虫转基因。鉴于使用寡核苷酸的某些核酸检测方法的敏感性，预期来源于如SEQ ID NO：2中的任意者中所示出的序列的寡核苷酸可用于在来源于汇集来源的商品产品中检测相应嵌合杀昆虫蛋白质，其中仅一部分商品产品来源于含有SEQ ID NO：2中的任意者的转基因植物。

实施例

鉴于上文，本领域技术人员应了解，以下公开实施方案仅代表本发明，本发明可以用多种形式体现。因此，本文中所公开的具体结构和功能细节不应理解为具有限制性。

实施例1

鳞翅目活性新型嵌合杀昆虫蛋白质编码序列的生成和克隆

本实施例说明新型嵌合杀昆虫蛋白质的生成以及所述嵌合杀昆虫蛋白质的克隆和表达。

由用于产生编码新型嵌合杀昆虫蛋白质的多核苷酸序列的已知Cry蛋白质基因构建了重组核酸序列。将所得多核苷酸序列克隆至苏云金芽孢杆菌(Bt)表达质粒载体中。在证实多核苷酸序列之后，将所述表达质粒转化至Bt中并且表达。测定所表达的新型嵌合蛋白质的制剂对各种鳞翅目害虫的活性。

产生了许多编码嵌合杀昆虫蛋白质的多核苷酸序列并且在生物测定中加以测试。不是所有嵌合杀昆虫蛋白质都显示出活性。仅基于其在生物测定中显示的对特定鳞翅目的活性来选择一些嵌合杀昆虫蛋白质。还基于原始嵌合杀昆虫蛋白质TIC867和TIC868产生了引入氨基酸取代或替代原毒素结构域的氨基酸变体。本发明的嵌合杀昆虫蛋白质的组分(结构域I、II和III，以及原毒素)提供于表1中。还提供了TIC868变体中相对于原始TIC868蛋白质序列的氨基酸取代。

表1.新型嵌合杀虫蛋白质和其组分。

*使用标准IUPAC氨基酸代码来标识氨基酸突变。参见IUPAC-IUB JointCommission on Biochemical Nomenclature.Nomenclature and Symbolism for AminoAcids and Peptides.Eur.J.Biochem.138：9-37(1984)。第一个氨基酸序列缩写表示给定支架蛋白质中的原始氨基酸，数字表示所述氨基酸的位置，而第二个氨基酸序列缩写表示改进的变体蛋白质中放在该位置上的氨基酸。

实施例2

新型嵌合杀昆虫蛋白质对鳞翅目害虫显示出活性

本实施例说明了对实施例1中所描述的嵌合杀昆虫蛋白质的测试和针对所述嵌合杀昆虫蛋白质观测到的鳞翅目活性。

在Bt中表达编码嵌合杀昆虫蛋白质的多核苷酸序列。然后针对已知是玉米、甘蔗、大豆和棉花以及其他作物植物的害虫的多种鳞翅目昆虫来测定所表达的嵌合杀昆虫蛋白质。具体来说，测定杀昆虫蛋白质对黎豆毛虫(VBC，黎豆夜蛾)、蔗螟(SCB，小蔗螟)、小玉米茎钻心虫(LSCB，南美玉米苗斑螟)、玉米穗虫(CEW，玉米穗夜蛾)、烟草芽虫(TBW，烟芽夜蛾)、大豆尺蠖(SBL，大豆夜蛾)、黑粘虫(BLAW，考斯夜蛾)、南方粘虫(SAW，亚热带粘虫)、草地粘虫(FAW，草地贪夜蛾)、甜菜粘虫(BAW，甜菜夜蛾)、古棉铃虫(OBW，棉铃实夜蛾)、东方叶虫(OLW，斜纹夜蛾)、粉红棉铃虫(PBW，棉红铃虫)、黑切根虫(BCW，球菜夜蛾)、西南玉米钻心虫(SWCB，西南玉米螟)、斑点棉铃虫(SBW，翠纹金刚钻)和欧洲玉米钻心虫(ECB，欧洲玉米螟)的活性。玉米穗虫(CEW，玉米穗夜蛾)也称为大豆荚虫(SPW)和棉铃虫(CBW)。通过死亡率与发育迟缓评分以及MIC50评分的组合来测定活性。MIC50是指蜕变抑制浓度，其中将死幼虫和L1幼虫(未能蜕变至第二龄的幼虫)都考虑至所述评分中。表2示出了每一种嵌合杀昆虫蛋白质的活性。‘+’符号表示对特定昆虫害虫观测到的活性。

如以上表2中可见，大部分嵌合杀昆虫蛋白质对一种或多种鳞翅目害虫物种表现出活性。

实施例3

合成编码嵌合杀昆虫蛋白质的基因并且用于在植物中表达

本实施例说明合成编码嵌合杀昆虫蛋白质的多核苷酸以便在植物中表达。

构建合成编码序列以用于在植物中表达嵌合杀昆虫蛋白质。根据美国专利5,500，365中大体描述的方法来设计并合成所述合成序列，从而避免某些有害的问题序列，诸如ATTTA和富A/T植物聚腺苷酸化序列，同时保留嵌合杀昆虫蛋白质的氨基酸序列。表3中列出了用于在植物中表达的编码所述嵌合杀昆虫蛋白质的这些基因的核苷酸序列。

表3.设计用于在植物中使用的编码嵌合杀昆虫蛋白质的多核苷酸序列。

实施例4

用于在植物中表达嵌合杀昆虫蛋白质的表达盒

本实施例说明包含设计用于在植物中使用的编码嵌合杀昆虫蛋白质的多核苷酸序列的表达盒的构建。

用表3中提供的设计用于植物表达的编码嵌合杀昆虫蛋白质的多核苷酸序列构建了多种植物表达盒。此类表达盒可用于在植物原生质体中暂时表达或转化植物细胞。根据蛋白质在细胞内的最终落点来设计典型表达盒。用允许蛋白质被翻译并且保留在细胞溶质中的方式设计一组表达盒。设计具有与毒素蛋白质连续的转运肽以允许靶向细胞的细胞器(诸如叶绿体或质粒)的另一组表达盒。以5′端开始用启动子设计所有表达盒，其可以由可操作地连接以加强转基因的表达的多个启动子元件、增强子元件或本领域技术人员已知的其他表达元件构成。启动子序列通常连续跟随有相对于启动子处于3′的一或多个前导序列。相对于前导序列，内含子序列通常提供在3′以改进转基因的表达。相对于可操作地连接的启动子、前导序列和内含子构形，毒素或转运肽的编码序列和毒素的编码序列通常位于3′。3′UTR序列通常提供在编码序列的3′，以促进转录终止并且提供对所得转录产物的聚腺苷酸化非常重要的序列。以上所描述的所有元件都可操作地连接并且依次布置，通常有为了构建表达盒而设的其他序列。

实施例5

嵌合杀昆虫蛋白质在经稳定转化的玉米中的鳞翅目活性

本实施例说明嵌合杀昆虫蛋白质当表达在玉米植物中并且作为食物提供给相应玉米昆虫害虫时对鳞翅目害虫表现出的抑制活性。

使用土壤杆菌介导的转化方法，用实施例4中所描述的二元转化载体转化玉米品种LH244。通过本领域中已知的方法诱导经转化的细胞以形成植物。以类似于美国专利No.8,344,207中所描述的那些生物测定的方式，使用植物叶盘进行生物测定。使用未转化的LH244植物来获得用作阴性对照的组织。针对玉米穗虫(CEW，玉米穗夜蛾)、草地粘虫(FAW，草地贪夜蛾)、黑切根虫(BCW，球菜夜蛾)和西南玉米钻心虫(SWCB，西南玉米螟)评价来自每一个二元载体的多个转化事件。

对R₀和F₁代转基因植物进行叶盘生物测定。另外，对受鳞翅目昆虫害虫侵扰的表达某些嵌合杀昆虫蛋白质的完整转基因F₁植物，评价叶损伤评级。还评价了表达TIC860和TIC868的F₁转基因事件在农田中对FAW、CEW和SWCB的活性。表4中示出了测定结果。‘+’符号表示对特定昆虫害虫观测到的活性。如表4中可见，大部分嵌合杀昆虫蛋白质和许多嵌合杀昆虫蛋白质变体对一种或多种鳞翅目害虫物种显示出活性。

实施例6

所述嵌合杀昆虫蛋白质在经稳定转化的大豆中的鳞翅目活性

本实施例说明嵌合杀昆虫蛋白质当表达在大豆植物中并且作为食物提供给相应昆虫害虫时对鳞翅目害虫表现出的抑制活性。

重新设计所选嵌合杀昆虫蛋白质的编码序列以用于植物表达，克隆至二元植物转化载体中，并且用于转化大豆植物细胞。所述植物转化载体包含用于如实施例4中所描述来表达嵌合杀昆虫蛋白质的第一转基因盒和用于使用壮观霉素选择来选择经转化的植物细胞的第二转基因盒。在一些情况下，诸如在TIC1100、TIC860和TIC836的情况下，将叶绿体转运肽编码序列可操作地连接至嵌合杀昆虫编码序列。用靶向和非靶向TIC1100、TIC860和TIC836的质粒进行测定。以下表5示出了嵌合杀昆虫蛋白质和TIC867变异嵌合杀昆虫蛋白质以及用于在经稳定转化的大豆中表达的相关编码序列。

使用以上所描述的二元转化载体通过土壤杆菌介导的转化来转化大豆植物细胞。诱导所得经转化的植物细胞以形成完整大豆植物。收集叶组织并且用于如实施例5中所描述的生物测定中，或替代地，将冻干组织用于昆虫食物中以用于生物测定。对FAW、南方粘虫(SAW，亚热带粘虫)、大豆尺蠖(SBL，大豆夜蛾)、大豆荚虫(SPW，玉米穗夜蛾)、黎豆毛虫(VBC，黎豆夜蛾)、烟草芽虫(TBW，烟芽夜蛾)、黑粘虫(BLAW，考斯夜蛾)、小玉米茎钻心虫(LSCB，南美玉米苗斑螟)和古棉铃虫(OBW，棉铃实夜蛾)进行生物测定。

表5示出了每一种杀昆虫蛋白质在R₀代植物中对所选鳞翅目物种的活性，其中‘+’表示活性。如表5中可见，经稳定转化的大豆中所表达的每一种嵌合杀昆虫蛋白质对多个鳞翅目物种显示出活性。特别值得注意的是，TIC867变体TIC867_23显示了对SPW的活性。

表5.来自经稳定转化的R₀大豆叶组织的嵌合杀昆虫蛋白质的生物测定活性。

允许所选转化事件自花授粉，并且种植所得种子。从R₁代植物收获叶组织并且用于饲喂生物测定。测定表达TIC1100、TIC860、TIC867、TIC868、TIC869和TIC836的R₁植物对SAW、SBL、SPW和VBC的活性。表6示出了在这些测试中所观测到的活性。‘+’符号表示对特定昆虫害虫观测到的活性。如表6中所显示，来自R₁代植物的大部分表达的嵌合杀昆虫蛋白质对一种或多种鳞翅目物种显示了活性。

表6.来自经稳定转化的R₁大豆叶组织的嵌合杀昆虫蛋白质的生物测定活性。

毒素	SAW	SBL	SPW	VBC
					TIC1100	+	+	+
TIC860	+	+		+
					TIC867	+
TIC868	+	+		+
					TIC869	+	+	+
TIC836	+	+		+

表7显示在网室中用表达TIC1100、TIC860和TIC836的经稳定转化的R₁代大豆植物进行农田测试的结果。在网室中用于侵扰植物的物种包括SAW、SBL和SPW。抗性定义为小于或等于百分之十五的大豆植物发生脱叶。在这些网笼试验中观测到的抗性与表6中提供的在R₁代大豆叶组织测定中观测到的抗性一致。‘+’符号表示对特定昆虫害虫观测到的活性。

表7.网室农田测试中所测试的R₁代大豆中表达的TIC1100、TIC860和TIC836的活性谱。

毒素	SAW	SBL	SPW
				TIC1100	+	+
TIC860	+	+
				TIC836	+	+

还在阿根廷的两个不同的地方阿塞维多和冯特左拉(Fontezuela)在网室中用表达TIC867和TIC869的经稳定转化的R₁代大豆植物进行农田测试。用于在网室中侵扰植物的物种包括南美棉铃虫(SABW，南美棉铃虫)、VBC、BLAW和向日葵尺蠖(SFL，薄荷灰夜蛾)。抗性定义为小于或等于百分之十五的大豆植物发生脱叶。以下表8示出了观测到的抗性。‘+’符号表示对特定昆虫害虫观测到的活性。如表8中所显示，表达TIC867的转基因大豆植物对BLAW和VBC显示了抗性。表达TIC869的转基因大豆植物对SABW、SFL、BLAW和VBC显示了抗性。

表8.网室农田测试中所测试的R₁代大豆中表达的TIC867和TIC869的活性谱。

实施例7

嵌合杀昆虫蛋白质在经稳定转化的棉花中的鳞翅目活性

本实施例说明嵌合杀昆虫蛋白质当表达在棉花植物中并且作为食物提供给相应昆虫害虫时对鳞翅目害虫表现出的抑制活性。

重新设计所选嵌合杀昆虫蛋白质的编码序列以用于植物表达，克隆至二元植物转化载体中，并且用于转化棉花植物细胞。所得二元载体类似于实施例4中所描述的那些，并且被用于表达靶向和非靶向TIC860(编码序列：SEQ ID NO：6；蛋白质序列：SEQ ID NO：7)、TIC867(编码序列：SEQ ID NO：9；蛋白质序列：SEQ ID NO：10)、TIC868(编码序列：SEQ IDNO：27；蛋白质序列：SEQ ID NO：28)和TIC867_23(编码序列：SEQ ID NO：20；蛋白质序列：SEQ ID NO：23)的质粒。

通过土壤杆菌介导的转化方法来转化棉花植物细胞。诱导经转化的棉花细胞以形成完整植物。如实施例5中所描述将棉花叶组织用于对棉铃虫(CBW，玉米穗夜蛾)、FAW、TBW和SBL进行生物测定。表9示出了TIC860、TIC867和TIC868在经稳定转化的R₀代棉花中对这些鳞翅目物种的观测活性，其中‘+’表示活性。如表9中可见，TIC860、TIC867和TIC868在经稳定转化的R₀代棉花中对两种或更多种鳞翅目害虫物种显示了活性。

表9.来自经稳定转化的R₀棉花叶组织的TIC860、TIC867和TIC868的生物测定活性。

毒素	CBW	FAW	TBW	SBL
					TIC860	+	+
TIC867	+	+	+	NT
					TIC868	+	+

所选转化事件被用于产生R₁种子。测定表达TIC860、TIC867和TIC868的R₁植物对CBW、FAW、TBW和SBL的抗性。将叶、蕾和蒴组织用于测定。表10示出了在这些测试中观测到的活性。‘+’符号表示对特定昆虫害虫观测到的活性。如表10中所显示，TIC860在叶组织中对FAW显示了活性。此外，嵌合杀昆虫蛋白质TIC867在叶、蕾和蒴组织中对CBW和FAW以及在叶中对TBW和SBL显示了活性。嵌合杀昆虫蛋白质TIC868在叶、蕾和蒴组织中对FAW以及在叶中对TBW和SBL显示了活性。

表10.来自经稳定转化的R₁棉花叶组织的嵌合杀昆虫蛋白质的生物测定活性。

本文中所公开和要求保护的所有组合物都可以在不过度实验的情况下根据本公开内容来制造和执行。尽管已经依据上述说明性实施方案描述了本发明的组合物，但本领域技术人员将显而易见，可以在不背离本发明的真实原理、精神和范围的情况下对本文中所描述的组合物加以变化、改变、修改和变更。更具体来说，将显而易见，在化学上和生理学上相关的某些药剂可以取代本文中所描述的药剂，同时将实现相同或类似的结果。对本领域技术人员显而易见的所有此类取代和修改都被认为在如所附权利要求书所定义的本发明精神、范围和原理内。

本说明书中所引用的所有出版物和公开的专利文件都以引用的方式并入本文中，达到与明确地且个别地指示每一个别出版物或专利申请以引用的方式并入相同的程度。

序列表

<110> Monsanto Technology LLC

Baum, James A

Cerruti, Thomas A

Dart, Crystal L

English, Leigh H

Fu, Xiaoran

Guzov, Victor M

Howe, Arlene R

Morgenstern, Jay P

Roberts, James K

Salvador, Sara A

Wang, Jinling

<120> 对鳞翅目害虫具有毒性或抑制性的嵌合杀昆虫蛋白质

<130> P34230WO00/0022270.00098

<150> US 62/064989

<151> 2014-10-16

<160> 53

<170> PatentIn version 3.5

<210> 1

<211> 3570

<212> DNA

<213> 人工的

<220>

<223> 用于在细菌细胞中表达的编码TIC1100的重组核苷酸序列。

<400> 1

atggagatag tgaataatca gaatcaatgc gtgccttata attgtttgaa taatcccgaa 60

atcgaaatat tagaaggcgg aagaatatca gttggtaata ccccaattga tatttctctt 120

tcgcttactc agtttctttt gagtgaattt gtcccaggtg cggggtttgt attaggatta 180

attgatttaa tatggggatt tgtaggtcct tcccaatggg acgcatttct tgctcaagtg 240

gaacagttaa ttaaccaaag aatagcagaa gctgtaagaa atacagcaat tcaggaatta 300

gagggaatgg cacgggttta tagaacctat gctactgctt ttgctgagtg ggaaaaagct 360

cctgatgacc cagagctaag agaagcacta cgtacacaat ttacagcaac tgagacttat 420

ataagtggaa gaatatccgt tttaaaaatt caaacttttg aagtacagct gttatcagtg 480

tttgcccaag ctgcaaattt acatttatct ttattaagag acgttgtgtt ttttgggcaa 540

agatggggtt tttcaacgac aaccgtaaat aattactaca atgatttaac agaagggatt 600

agtacctata cagattatgc tgtacgctgg tacaatacgg gattagaacg tgtatgggga 660

ccggattcta gagattgggt aaggtataat caatttagaa gagaattaac actaactgta 720

ttagatatcg ttgctctgtt cccgaattat gatagtagaa gatatccaat tcgaacagtt 780

tcccaattaa caagagaaat ttatacaaac ccagtattag aaaattttga tggtagtttt 840

cgaggctcgg ctcagggcat agaaagaagt attaggagtc cacatttgat ggatatactt 900

aacagtataa ccatctatac ggatgctcat aggggttatt attattggtc agggcatcaa 960

ataatggctt ctcctgtcgg tttttcgggg ccagaattca cgtttccgct atatggaacc 1020

atgggaaatg cagctccaca acaacgtatt gttgctcaac taggtcaggg cgtgtataga 1080

acattatcgt ccactttata tagaagacct tttaatatag ggataaataa tcaacaacta 1140

tctgttcttg acgggacaga atttgcttat ggaacctcct caaatttgcc atccgctgta 1200

tacagaaaaa gcggaacggt agattcgctg gatgaaatac cgccacagaa taacaacgtg 1260

ccacctaggc aaggatttag tcatcgatta agccatgttt caatgtttcg ttcaggcttt 1320

agtaatagta gtgtaagtat aataagagct cctatgttct cttggataca tcgtagtgct 1380

gaatttaata atataattgc atcggatagt attaatcaaa tacctttagt gaaaggattt 1440

agagtttggg ggggcacctc tgtcattaca ggaccaggat ttacaggagg ggatatcctt 1500

cgaagaaata cctttggtga ttttgtatct ctacaagtca atattaattc accaattacc 1560

caaagatacc gtttaagatt tcgttacgct tccagtaggg atgcacgagt tatagtatta 1620

acaggagcgg catccacagg agtgggaggc caagttagtg taaatatgcc tcttcagaaa 1680

actatggaaa taggggagaa cttaacatct agaacattta gatataccga ttttagtaat 1740

cctttttcat ttagagctaa tccagatata attgggataa gtgaacaacc tctatttggt 1800

gcaggttcta ttagtagcgg tgaactttat atagataaaa ttgaaattat tctagcagat 1860

gcaacatttg aagcagaatc tgatttagaa agagcgcaga aggcggtgaa tgcgctgttt 1920

acgtctacaa accaactagg gctaaaaaca aatgtaacgg attatcatat tgatcaagtg 1980

tccaatttag ttacgtattt atcggatgaa ttttgtctgg atgaaaagcg agaattgtcc 2040

gagaaagtca aacatgcgaa gcgactcagt gatgaacgca atttactcca agattcaaat 2100

ttcaaagaca ttaataggca accagaacgt gggtggggcg gaagtacagg gattaccatc 2160

caaggagggg atgacgtatt taaagaaaat tacgtcacac tatcaggtac ctttgatgag 2220

tgctatccaa catatttgta tcaaaaaatc gatgaatcaa aattaaaagc ctttacccgt 2280

tatcaattaa gagggtatat cgaagatagt caagacttag aaatctattt aattcgctac 2340

aatgcaaaac atgaaacagt aaatgtgcca ggtacgggtt ccttatggcc gctttcagcc 2400

caaagtccaa tcggaaagtg tggagagccg aatcgatgcg cgccacacct tgaatggaat 2460

cctgacttag attgttcgtg tagggatgga gaaaagtgtg cccatcattc gcatcatttc 2520

tccttagaca ttgatgtagg atgtacagac ttaaatgagg acctaggtgt atgggtgatc 2580

tttaagatta agacgcaaga tgggcacgca agactaggga atctagagtt tctcgaagag 2640

aaaccattag taggagaagc gctagctcgt gtgaaaagag cggagaaaaa atggagagac 2700

aaacgtgaaa aattggaatg ggaaacaaat atcgtttata aagaggcaaa agaatctgta 2760

gatgctttat ttgtaaactc tcaatatgat caattacaag cggatacgaa tattgccatg 2820

attcatgcgg cagataaacg tgttcatagc attcgagaag cttatctgcc tgagctgtct 2880

gtgattccgg gtgtcaatgc ggctattttt gaagaattag aagggcgtat tttcactgca 2940

ttctccctat atgatgcgag aaatgtcatt aaaaatggtg attttaataa tggcttatcc 3000

tgctggaacg tgaaagggca tgtagatgta gaagaacaaa acaaccaacg ttcggtcctt 3060

gttgttccgg aatgggaagc agaagtgtca caagaagttc gtgtctgtcc gggtcgtggc 3120

tatatccttc gtgtcacagc gtacaaggag ggatatggag aaggttgcgt aaccattcat 3180

gagatcgaga acaatacaga cgaactgaag tttagcaact gcgtagaaga ggaaatctat 3240

ccaaataaca cggtaacgtg taatgattat actgtaaatc aagaagaata cggaggtgcg 3300

tacacttctc gtaatcgagg atataacgaa gctccttccg taccagctga ttatgcgtca 3360

gtctatgaag aaaaatcgta tacagatgga cgaagagaga atccttgtga atttaacaga 3420

gggtataggg attacacgcc actaccagtt ggttatgtga caaaagaatt agaatacttc 3480

ccagaaaccg ataaggtatg gattgagatt ggagaaacgg aaggaacatt tatcgtggac 3540

agcgtggaat tactccttat ggaggaatga 3570

<210> 2

<211> 3570

<212> DNA

<213> 人工的

<220>

<223> 设计用于在植物细胞中表达的编码TIC1100的合成核苷酸序列。

<400> 2

atggagattg tgaacaacca gaaccagtgc gttccttaca actgcttgaa caaccctgag 60

attgagattc ttgagggtgg tagaatttct gttggcaaca ctcctattga catctctttg 120

agtttgactc aattcttgtt gagtgagttc gttcctggtg ctggtttcgt cttgggtttg 180

attgatttga tttggggttt cgttggtcct agtcaatggg atgctttctt ggctcaagtt 240

gagcaattga ttaaccagag gatcgctgag gctgtgagga acactgctat tcaagagttg 300

gagggtatgg ctagagttta cagaacttac gctactgctt tcgctgagtg ggagaaggct 360

cctgatgacc ctgagttgag ggaggctttg agaactcaat tcactgctac tgagacttac 420

atcagtggta gaatcagtgt cttgaagatt caaactttcg aggttcaatt gctttctgtg 480

ttcgctcaag ctgcaaactt gcacttgtct ttgcttagag atgttgtgtt ctttggtcaa 540

agatggggtt tctccactac taccgtgaac aattactaca acgatttgac tgagggtatt 600

tctacttaca ctgattacgc tgttagatgg tacaacactg gtttggagag agtttggggt 660

ccagattcca gagattgggt cagatacaac cagttcagaa gggagttgac tttgactgtc 720

ttggacattg ttgctctctt ccctaactac gatagtcgtc gttaccctat tagaactgtt 780

tctcaactta ctagggaaat ctacactaac cctgttcttg agaacttcga tggtagtttc 840

cgtggtagtg ctcaagggat tgagcgttct attcgttctc ctcatcttat ggacattctt 900

aactctatta ctatctacac tgatgctcat cgtggttact attactggtc tggtcatcaa 960

attatggcta gtcctgttgg tttcagtggt cctgagttca ctttccctct ttacggtact 1020

atgggcaacg ctgcacctca acagaggatc gttgctcaac ttggtcaagg tgtttacagg 1080

actctttctt caacccttta caggcgtcct ttcaacattg ggatcaacaa ccagcagctt 1140

tctgttcttg atggaaccga gttcgcttac ggaacctctt caaaccttcc tagtgctgtt 1200

tacaggaagt ctggaaccgt tgacagtctt gatgagattc caccgcagaa caataacgtt 1260

ccacccaggc aaggcttcag tcataggctt tctcatgttt ctatgttccg ctctggattc 1320

agcaactctt cagtttctat tatcagggct ccaatgttct cgtggattca taggtctgcc 1380

gagttcaaca acattatcgc ttccgatagc attaaccaga ttccacttgt taagggattc 1440

cgtgtttggg gaggcacctc tgttattacc ggaccaggct tcaccggagg cgacattctt 1500

cgtcgtaaca ccttcggaga tttcgtttca cttcaagtga acattaactc accaatcacc 1560

cagcgctaca ggcttcgctt ccgctacgca tcatccaggg atgcaagggt gatcgtgctt 1620

accggagcag cctcaaccgg agtgggaggc caagtgagcg tgaacatgcc acttcagaag 1680

acgatggaga tcggcgagaa ccttacctca agaacctttc gttacaccga tttcagcaac 1740

ccattcagct ttcgtgcaaa cccagacatc atagggatct cagagcagcc actgtttgga 1800

gctggatcaa tctcatccgg agagctttac atcgacaaga tcgagatcat actcgcagat 1860

gcaaccttcg aggctgagag cgatctggag cgtgcacaga aggcagtgaa cgcactcttt 1920

acctctacca accagctcgg actcaagacc aacgtgaccg attaccacat cgaccaagtg 1980

agcaacctcg tgacctacct ctcagatgag ttctgcttgg atgagaaacg cgaactcagc 2040

gagaaggtga agcacgcaaa gcgtctctca gatgagcgta acctcctcca ggatagcaat 2100

ttcaaggaca tcaatcgtca gccagagcgt ggatggggag gctcaaccgg aatcaccatc 2160

cagggaggcg atgatgtgtt taaggagaat tacgtgacac tctccggaac attcgatgag 2220

tgctacccaa catacctcta tcagaagatc gacgagtcca agctcaaggc gttcacccgt 2280

tatcagctcc gtggctacat cgaggatagt caagacctgg aaatctacct catccgctac 2340

aatgcaaagc acgagacagt gaatgtgcca ggaacaggct ccctctggcc actctccgca 2400

cagtctccaa tcggcaagtg cggcgagcca aatcgctgcg cgccacacct ggagtggaat 2460

cccgacctgg actgctcctg ccgcgacggc gagaagtgcg cccaccactc ccaccacttt 2520

agcctggaca tcgacgtggg ctgtacagac ctgaatgagg atctgggcgt gtgggtgatc 2580

tttaagatca agacacagga cggccacgcc cgcctgggca atctggagtt tctggaggag 2640

aagcctctgg tgggcgaagc cctggcccgc gtgaagcgcg ccgagaagaa atggcgcgac 2700

aaacgcgaga aactggaatg ggaaacaaac atcgtgtaca aagaagccaa agaatccgtg 2760

gacgccctat ttgtgaactc ccagtatgac cagctacagg ccgacacaaa catcgcgatg 2820

atccacgctg cggacaagcg cgtgcactcc atacgcgaag cctatctacc cgaactatcc 2880

gtgatacccg gcgtcaatgc cgcgatcttt gaagaattgg aaggccgcat cttcacagcc 2940

tttagcctct atgacgcccg aaatgtcatc aagaatggcg actttaacaa tgggctatcc 3000

tgttggaatg tcaaagggca cgtggacgtc gaagagcaga acaatcagcg atccgtctta 3060

gtcgtacccg aatgggaagc cgaagtctcc caggaagtcc gagtctgtcc tggtagaggt 3120

tacatcttga gagtgactgc ttacaaggag ggttacggtg agggatgcgt gactattcac 3180

gagattgaga acaacactga tgagttgaag ttcagtaact gcgtggagga ggaaatctac 3240

cccaacaaca ctgtgacttg taacgattac accgtgaacc aggaggaata cggaggcgct 3300

tacacctcca gaaaccgtgg atacaatgag gctccctcgg tccccgctga ttatgcctcc 3360

gtctatgagg agaagtccta caccgatgga aggcgcgaga atccctgcga gttcaatcgc 3420

ggctatcgag actacactcc gctacccgtt ggctatgtca caaaggaact ggaatacttc 3480

ccggaaacag acaaagtctg gatcgaaatc ggcgaaacag aagggacgtt catagtcgat 3540

agcgtagaac ttctccttat ggaagaatga 3570

<210> 3

<211> 3570

<212> DNA

<213> 人工的

<220>

<400> 3

atggagattg tgaacaacca gaaccagtgc gttccttaca actgcttgaa caaccctgag 60

attgagattc ttgagggtgg tagaatttct gttggcaaca ctcctattga catctctttg 120

agtttgactc aattcttgtt gagtgagttc gttcctggtg ctggtttcgt cttgggtttg 180

attgatttga tttggggttt cgttggtcct agtcaatggg atgctttctt ggctcaagtt 240

gagcaattga ttaaccagag gatcgctgag gctgtgagga acactgctat tcaagagttg 300

gagggtatgg ctagagttta cagaacttac gctactgctt tcgctgagtg ggagaaggct 360

cctgatgacc ctgagttgag ggaggctttg agaactcaat tcactgctac tgagacttac 420

atcagtggta gaatcagtgt cttgaagatt caaactttcg aggttcaatt gctttctgtg 480

ttcgctcaag ctgcaaactt gcacttgtct ttgcttagag atgttgtgtt ctttggtcaa 540

agatggggtt tctccactac taccgtgaac aattactaca acgatttgac tgagggtatt 600

tctacttaca ctgattacgc tgttagatgg tacaacactg gtttggagag agtttggggt 660

ccagattcca gagattgggt cagatacaac cagttcagaa gggagttgac tttgactgtc 720

ttggacattg ttgctctctt ccctaactac gatagtcgtc gttaccctat tagaactgtt 780

tctcaactta ctagggaaat ctacactaac cctgttcttg agaacttcga tggtagtttc 840

cgtggtagtg ctcaagggat tgagcgttct attcgttctc ctcatcttat ggacattctt 900

aactctatta ctatctacac tgatgctcat cgtggttact attactggtc tggtcatcaa 960

attatggcta gtcctgttgg tttcagtggt cctgagttca ctttccctct ttacggtact 1020

atgggcaacg ctgcacctca acagaggatc gttgctcaac ttggtcaagg tgtttacagg 1080

actctttctt caacccttta caggcgtcct ttcaacattg ggatcaacaa ccagcagctt 1140

tctgttcttg atggaaccga gttcgcttac ggaacctctt caaaccttcc tagtgctgtt 1200

tacaggaagt ctggaaccgt tgacagtctt gatgagattc caccgcagaa caataacgtt 1260

ccacccaggc aaggcttcag tcataggctt tctcatgttt ctatgttccg ctctggattc 1320

agcaactctt cagtttctat tatcagggct ccaatgttct cgtggattca taggtctgcc 1380

gagttcaaca acattatcgc ttccgatagc attaaccaga ttccacttgt taagggattc 1440

cgtgtttggg gaggcacctc tgttattacc ggaccaggct tcaccggagg cgacattctt 1500

cgtcgtaaca ccttcggaga tttcgtttca cttcaagtga acattaactc accaatcacc 1560

cagcgctaca ggcttcgctt ccgctacgca tcatccaggg atgcaagggt gatcgtgctt 1620

accggagcag cctcaaccgg agtgggaggc caagtgagcg tgaacatgcc acttcagaag 1680

acgatggaga tcggcgagaa ccttacctca agaacctttc gttacaccga tttcagcaac 1740

ccattcagct ttcgtgcaaa cccagacatc atagggatct cagagcagcc actgtttgga 1800

gctggatcaa tctcatccgg agagctttac atcgacaaga tcgagatcat actcgcagat 1860

gcaaccttcg aggctgagag cgatctggag cgtgcacaga aggcagtgaa cgcactcttt 1920

acctctacca accagctcgg actcaagacc aacgtgaccg attaccacat cgaccaagtg 1980

agcaacctcg tgacctacct ctcagatgag ttctgcttgg atgagaaacg cgaactcagc 2040

gagaaggtga agcacgcaaa gcgtctctca gatgagcgta acctcctcca ggatagcaat 2100

ttcaaggaca tcaatcgtca gccagagcgt ggatggggag gctcaaccgg aatcaccatc 2160

cagggaggcg atgatgtgtt taaggagaat tacgtgacac tctccggaac attcgatgag 2220

tgctacccaa catacctcta tcagaagatc gacgagtcca agctcaaggc gttcacccgt 2280

tatcagctcc gtggctacat cgaggatagt caagacctgg aaatctacct catccgctac 2340

aatgcaaagc acgagacagt gaatgtacca ggaacaggct ccctctggcc actctccgca 2400

cagtctccaa tcggcaagtg cggcgagcca aatcgctgcg cgccacacct ggagtggaat 2460

cccgacctgg actgctcctg ccgcgacggc gagaagtgcg cccaccactc ccaccacttt 2520

agcctggaca tcgacgtggg ctgtacagac ctgaatgagg atctgggcgt gtgggtgatc 2580

tttaagatca agacacagga cggccacgcc cgcctgggca atctggagtt tctggaggag 2640

aagcctctgg tgggcgaagc cctggcccgc gtgaagcgcg ccgagaagaa atggcgcgac 2700

aaacgcgaga aactggaatg ggaaacaaac atcgtgtaca aagaagccaa agaatccgtg 2760

gacgccctat ttgtgaactc ccagtatgac cagctacagg ccgacacaaa catcgcgatg 2820

atccacgctg cggacaagcg cgtgcactcc atacgcgaag cctatctacc cgaactatcc 2880

gtgatacccg gcgtcaatgc cgcgatcttt gaagaattgg aaggccgcat cttcacagcc 2940

tttagcctct atgacgcccg aaatgtcatc aagaatggcg actttaacaa tgggctatcc 3000

tgttggaatg tcaaagggca cgtggacgtc gaagagcaga acaatcagcg atccgtctta 3060

gtcgtacccg aatgggaagc cgaagtctcc caggaagtcc gagtctgtcc tggtagaggt 3120

tacatcttga gagtgactgc ttacaaggag ggttacggtg agggatgcgt gactattcac 3180

gagattgaga acaacactga tgagttgaag ttcagtaact gcgtggagga ggaaatctac 3240

cccaacaaca ctgtgacttg taacgattac accgtgaacc aggaggaata cggaggcgct 3300

tacacctcca gaaaccgtgg atacaatgag gctccctcgg tccccgctga ttatgcctcc 3360

gtctatgagg agaagtccta caccgatgga aggcgcgaga atccctgcga gttcaatcgc 3420

ggctatcgag actacactcc gctacccgtt ggctatgtca caaaggaact ggaatacttc 3480

ccggaaacag acaaagtctg gatcgaaatc ggcgaaacag aagggacgtt catagtcgat 3540

agcgtagaac ttctccttat ggaagaatga 3570

<210> 4

<211> 1189

<212> PRT

<213> 人工的

<220>

<223> 嵌合蛋白质TIC1100的氨基酸序列。

<400> 4

Met Glu Ile Val Asn Asn Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu

1 5 10 15

Asn Asn Pro Glu Ile Glu Ile Leu Glu Gly Gly Arg Ile Ser Val Gly

20 25 30

Asn Thr Pro Ile Asp Ile Ser Leu Ser Leu Thr Gln Phe Leu Leu Ser

35 40 45

Glu Phe Val Pro Gly Ala Gly Phe Val Leu Gly Leu Ile Asp Leu Ile

50 55 60

Trp Gly Phe Val Gly Pro Ser Gln Trp Asp Ala Phe Leu Ala Gln Val

65 70 75 80

Glu Gln Leu Ile Asn Gln Arg Ile Ala Glu Ala Val Arg Asn Thr Ala

85 90 95

Ile Gln Glu Leu Glu Gly Met Ala Arg Val Tyr Arg Thr Tyr Ala Thr

100 105 110

Ala Phe Ala Glu Trp Glu Lys Ala Pro Asp Asp Pro Glu Leu Arg Glu

115 120 125

Ala Leu Arg Thr Gln Phe Thr Ala Thr Glu Thr Tyr Ile Ser Gly Arg

130 135 140

Ile Ser Val Leu Lys Ile Gln Thr Phe Glu Val Gln Leu Leu Ser Val

145 150 155 160

Phe Ala Gln Ala Ala Asn Leu His Leu Ser Leu Leu Arg Asp Val Val

165 170 175

Phe Phe Gly Gln Arg Trp Gly Phe Ser Thr Thr Thr Val Asn Asn Tyr

180 185 190

Tyr Asn Asp Leu Thr Glu Gly Ile Ser Thr Tyr Thr Asp Tyr Ala Val

195 200 205

Arg Trp Tyr Asn Thr Gly Leu Glu Arg Val Trp Gly Pro Asp Ser Arg

210 215 220

Asp Trp Val Arg Tyr Asn Gln Phe Arg Arg Glu Leu Thr Leu Thr Val

225 230 235 240

Leu Asp Ile Val Ala Leu Phe Pro Asn Tyr Asp Ser Arg Arg Tyr Pro

245 250 255

Ile Arg Thr Val Ser Gln Leu Thr Arg Glu Ile Tyr Thr Asn Pro Val

260 265 270

Leu Glu Asn Phe Asp Gly Ser Phe Arg Gly Ser Ala Gln Gly Ile Glu

275 280 285

Arg Ser Ile Arg Ser Pro His Leu Met Asp Ile Leu Asn Ser Ile Thr

290 295 300

Ile Tyr Thr Asp Ala His Arg Gly Tyr Tyr Tyr Trp Ser Gly His Gln

305 310 315 320

Ile Met Ala Ser Pro Val Gly Phe Ser Gly Pro Glu Phe Thr Phe Pro

325 330 335

Leu Tyr Gly Thr Met Gly Asn Ala Ala Pro Gln Gln Arg Ile Val Ala

340 345 350

Gln Leu Gly Gln Gly Val Tyr Arg Thr Leu Ser Ser Thr Leu Tyr Arg

355 360 365

Arg Pro Phe Asn Ile Gly Ile Asn Asn Gln Gln Leu Ser Val Leu Asp

370 375 380

Gly Thr Glu Phe Ala Tyr Gly Thr Ser Ser Asn Leu Pro Ser Ala Val

385 390 395 400

Tyr Arg Lys Ser Gly Thr Val Asp Ser Leu Asp Glu Ile Pro Pro Gln

405 410 415

Asn Asn Asn Val Pro Pro Arg Gln Gly Phe Ser His Arg Leu Ser His

420 425 430

Val Ser Met Phe Arg Ser Gly Phe Ser Asn Ser Ser Val Ser Ile Ile

435 440 445

Arg Ala Pro Met Phe Ser Trp Ile His Arg Ser Ala Glu Phe Asn Asn

450 455 460

Ile Ile Ala Ser Asp Ser Ile Asn Gln Ile Pro Leu Val Lys Gly Phe

465 470 475 480

Arg Val Trp Gly Gly Thr Ser Val Ile Thr Gly Pro Gly Phe Thr Gly

485 490 495

Gly Asp Ile Leu Arg Arg Asn Thr Phe Gly Asp Phe Val Ser Leu Gln

500 505 510

Val Asn Ile Asn Ser Pro Ile Thr Gln Arg Tyr Arg Leu Arg Phe Arg

515 520 525

Tyr Ala Ser Ser Arg Asp Ala Arg Val Ile Val Leu Thr Gly Ala Ala

530 535 540

Ser Thr Gly Val Gly Gly Gln Val Ser Val Asn Met Pro Leu Gln Lys

545 550 555 560

Thr Met Glu Ile Gly Glu Asn Leu Thr Ser Arg Thr Phe Arg Tyr Thr

565 570 575

Asp Phe Ser Asn Pro Phe Ser Phe Arg Ala Asn Pro Asp Ile Ile Gly

580 585 590

Ile Ser Glu Gln Pro Leu Phe Gly Ala Gly Ser Ile Ser Ser Gly Glu

595 600 605

Leu Tyr Ile Asp Lys Ile Glu Ile Ile Leu Ala Asp Ala Thr Phe Glu

610 615 620

Ala Glu Ser Asp Leu Glu Arg Ala Gln Lys Ala Val Asn Ala Leu Phe

625 630 635 640

Thr Ser Thr Asn Gln Leu Gly Leu Lys Thr Asn Val Thr Asp Tyr His

645 650 655

Ile Asp Gln Val Ser Asn Leu Val Thr Tyr Leu Ser Asp Glu Phe Cys

660 665 670

Leu Asp Glu Lys Arg Glu Leu Ser Glu Lys Val Lys His Ala Lys Arg

675 680 685

Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Ser Asn Phe Lys Asp Ile

690 695 700

Asn Arg Gln Pro Glu Arg Gly Trp Gly Gly Ser Thr Gly Ile Thr Ile

705 710 715 720

Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val Thr Leu Ser Gly

725 730 735

Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln Lys Ile Asp Glu

740 745 750

Ser Lys Leu Lys Ala Phe Thr Arg Tyr Gln Leu Arg Gly Tyr Ile Glu

755 760 765

Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr Asn Ala Lys His

770 775 780

Glu Thr Val Asn Val Pro Gly Thr Gly Ser Leu Trp Pro Leu Ser Ala

785 790 795 800

Gln Ser Pro Ile Gly Lys Cys Gly Glu Pro Asn Arg Cys Ala Pro His

805 810 815

Leu Glu Trp Asn Pro Asp Leu Asp Cys Ser Cys Arg Asp Gly Glu Lys

820 825 830

Cys Ala His His Ser His His Phe Ser Leu Asp Ile Asp Val Gly Cys

835 840 845

Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Val Ile Phe Lys Ile Lys

850 855 860

Thr Gln Asp Gly His Ala Arg Leu Gly Asn Leu Glu Phe Leu Glu Glu

865 870 875 880

Lys Pro Leu Val Gly Glu Ala Leu Ala Arg Val Lys Arg Ala Glu Lys

885 890 895

Lys Trp Arg Asp Lys Arg Glu Lys Leu Glu Trp Glu Thr Asn Ile Val

900 905 910

Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala Leu Phe Val Asn Ser Gln

915 920 925

Tyr Asp Gln Leu Gln Ala Asp Thr Asn Ile Ala Met Ile His Ala Ala

930 935 940

Asp Lys Arg Val His Ser Ile Arg Glu Ala Tyr Leu Pro Glu Leu Ser

945 950 955 960

Val Ile Pro Gly Val Asn Ala Ala Ile Phe Glu Glu Leu Glu Gly Arg

965 970 975

Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala Arg Asn Val Ile Lys Asn

980 985 990

Gly Asp Phe Asn Asn Gly Leu Ser Cys Trp Asn Val Lys Gly His Val

995 1000 1005

Asp Val Glu Glu Gln Asn Asn Gln Arg Ser Val Leu Val Val Pro

1010 1015 1020

Glu Trp Glu Ala Glu Val Ser Gln Glu Val Arg Val Cys Pro Gly

1025 1030 1035

Arg Gly Tyr Ile Leu Arg Val Thr Ala Tyr Lys Glu Gly Tyr Gly

1040 1045 1050

Glu Gly Cys Val Thr Ile His Glu Ile Glu Asn Asn Thr Asp Glu

1055 1060 1065

Leu Lys Phe Ser Asn Cys Val Glu Glu Glu Ile Tyr Pro Asn Asn

1070 1075 1080

Thr Val Thr Cys Asn Asp Tyr Thr Val Asn Gln Glu Glu Tyr Gly

1085 1090 1095

Gly Ala Tyr Thr Ser Arg Asn Arg Gly Tyr Asn Glu Ala Pro Ser

1100 1105 1110

Val Pro Ala Asp Tyr Ala Ser Val Tyr Glu Glu Lys Ser Tyr Thr

1115 1120 1125

Asp Gly Arg Arg Glu Asn Pro Cys Glu Phe Asn Arg Gly Tyr Arg

1130 1135 1140

Asp Tyr Thr Pro Leu Pro Val Gly Tyr Val Thr Lys Glu Leu Glu

1145 1150 1155

Tyr Phe Pro Glu Thr Asp Lys Val Trp Ile Glu Ile Gly Glu Thr

1160 1165 1170

Glu Gly Thr Phe Ile Val Asp Ser Val Glu Leu Leu Leu Met Glu

1175 1180 1185

Glu

<210> 5

<211> 3672

<212> DNA

<213> 人工的

<220>

<223> 用于在细菌细胞中表达的编码TIC860的重组核苷酸序列。

<400> 5

atgacttcaa ataggaaaaa tgagaatgaa attataaatg ctttatcgat tccaacggta 60

tcgaatcctt ccacgcaaat gaatctatca ccagatgctc gtattgaaga tagcttgtgt 120

gtagccgagg tgaacaatat tgatccattt gttagcgcat caacagtcca aacgggtata 180

aacatagctg gtagaatatt gggcgtatta ggtgtgccgt ttgctggaca actagctagt 240

ttttatagtt ttcttgttgg ggaattatgg cctagtggca gagatccatg ggaaattttc 300

ctggaacatg tagaacaact tataagacaa caagtaacag aaaatactag gaatacggct 360

attgctcgat tagaaggtct aggaagaggc tatagatctt accagcaggc tcttgaaact 420

tggttagata accgaaatga tgcaagatca agaagcatta ttcttgagcg ctatgttgct 480

ttagaacttg acattactac tgctataccg cttttcagaa tacgaaatga agaagttcca 540

ttattaatgg tatatgctca agctgcaaat ttacacctat tattattgag agacgcatcc 600

ctttttggta gtgaatgggg gatggcatct tccgatgtta accaatatta ccaagaacaa 660

atcagatata cagaggaata ttctaaccat tgcgtacaat ggtataatac agggctaaat 720

aacttaagag ggacaaatgc tgaaagttgg ttgcggtata atcaattccg tagagaccta 780

acgttagggg tattagattt agtagcccta ttcccaagct atgatactcg cacttatcca 840

atcaatacga gtgctcagtt aacaagagaa atttatacag atccaattgg gagaacaaat 900

gcaccttcag gatttgcaag tacgaattgg tttaataata atgcaccatc gttttctgcc 960

atagaggctg ccattttcag gcctccgcat ctacttgatt ttccagaaca acttacaatt 1020

tacagtgcat caagccgttg gagtagcact caacatatga attattgggt gggacatagg 1080

cttaacttcc gcccaatagg agggacatta aatacctcaa cacaaggact tactaataat 1140

acttcaatta atcctgtaac attacagttt acgtctcgag acgtttatag aacagaatca 1200

aatgcaggga caaatatact atttactact cctgtgaatg gagtaccttg ggctagattt 1260

aattttataa accctcagaa tatttatgaa agaggcgcca ctacctacag tcaaccgtat 1320

cagggagttg ggattcaatt atttgattca gaaactgaat taccaccaga aacaacagaa 1380

cgaccaaatt atgaatcata tagtcataga ttatctcata taggactaat cataggaaac 1440

actttgagag caccagtcta ttcttggacg catcgtagtg cagatcgtac gaatacgatt 1500

ggaccaaata gaattaatca aataccttta gtgaaaggat ttagagtttg ggggggcacc 1560

tctgtcatta caggaccagg atttacagga ggggatatcc ttcgaagaaa tacctttggt 1620

gattttgtat ctctacaagt caatattaat tcaccaatta cccaaagata ccgtttaaga 1680

tttcgttacg cttccagtag ggatgcacga gttatagtat taacaggagc ggcatccaca 1740

ggagtgggag gccaagttag tgtaaatatg cctcttcaga aaactatgga aataggggag 1800

aacttaacat ctagaacatt tagatatacc gattttagta atcctttttc atttagagct 1860

aatccagata taattgggat aagtgaacaa cctctatttg gtgcaggttc tattagtagc 1920

ggtgaacttt atatagataa aattgaaatt attctagcag atgcaacatt tgaagcagaa 1980

tctgatttag aaagagcgca gaaggcggtg aatgcgctgt ttacgtctac aaaccaacta 2040

gggctaaaaa caaatgtaac ggattatcat attgatcaag tgtccaattt agttacgtat 2100

ttatcggatg aattttgtct ggatgaaaag cgagaattgt ccgagaaagt caaacatgcg 2160

aagcgactca gtgatgaacg caatttactc caagattcaa atttcaaaga cattaatagg 2220

caaccagaac gtgggtgggg cggaagtaca gggattacca tccaaggagg ggatgacgta 2280

tttaaagaaa attacgtcac actatcaggt acctttgatg agtgctatcc aacatatttg 2340

tatcaaaaaa tcgatgaatc aaaattaaaa gcctttaccc gttatcaatt aagagggtat 2400

atcgaagata gtcaagactt agaaatctat ttaattcgct acaatgcaaa acatgaaaca 2460

gtaaatgtgc caggtacggg ttccttatgg ccgctttcag cccaaagtcc aatcggaaag 2520

tgtggagagc cgaatcgatg cgcgccacac cttgaatgga atcctgactt agattgttcg 2580

tgtagggatg gagaaaagtg tgcccatcat tcgcatcatt tctccttaga cattgatgta 2640

ggatgtacag acttaaatga ggacctaggt gtatgggtga tctttaagat taagacgcaa 2700

gatgggcacg caagactagg gaatctagag tttctcgaag agaaaccatt agtaggagaa 2760

gcgctagctc gtgtgaaaag agcggagaaa aaatggagag acaaacgtga aaaattggaa 2820

tgggaaacaa atatcgttta taaagaggca aaagaatctg tagatgcttt atttgtaaac 2880

tctcaatatg atcaattaca agcggatacg aatattgcca tgattcatgc ggcagataaa 2940

cgtgttcata gcattcgaga agcttatctg cctgagctgt ctgtgattcc gggtgtcaat 3000

gcggctattt ttgaagaatt agaagggcgt attttcactg cattctccct atatgatgcg 3060

agaaatgtca ttaaaaatgg tgattttaat aatggcttat cctgctggaa cgtgaaaggg 3120

catgtagatg tagaagaaca aaacaaccaa cgttcggtcc ttgttgttcc ggaatgggaa 3180

gcagaagtgt cacaagaagt tcgtgtctgt ccgggtcgtg gctatatcct tcgtgtcaca 3240

gcgtacaagg agggatatgg agaaggttgc gtaaccattc atgagatcga gaacaataca 3300

gacgaactga agtttagcaa ctgcgtagaa gaggaaatct atccaaataa cacggtaacg 3360

tgtaatgatt atactgtaaa tcaagaagaa tacggaggtg cgtacacttc tcgtaatcga 3420

ggatataacg aagctccttc cgtaccagct gattatgcgt cagtctatga agaaaaatcg 3480

tatacagatg gacgaagaga gaatccttgt gaatttaaca gagggtatag ggattacacg 3540

ccactaccag ttggttatgt gacaaaagaa ttagaatact tcccagaaac cgataaggta 3600

tggattgaga ttggagaaac ggaaggaaca tttatcgtgg acagcgtgga attactcctt 3660

atggaggaat ag 3672

<210> 6

<211> 3672

<212> DNA

<213> 人工的

<220>

<223> 设计用于在植物细胞中表达的编码TIC860的合成核苷酸序列。

<400> 6

atgaccagca accggaagaa cgagaacgag atcatcaacg ccctgagcat cccgaccgtg 60

agcaacccta gcacccagat gaacctgagc cctgacgctc gcatcgagga ctccctctgc 120

gtggctgagg tgaacaacat cgacccgttc gtgtccgcct ccaccgtgca gaccggcatc 180

aacatcgcgg gccgcatcct cggcgtgctc ggcgtgccct ttgcgggcca gctcgcctcc 240

ttctactcct tcctcgtggg agagctgtgg ccctccggcc gcgacccgtg ggagatcttc 300

ctggagcacg tggagcagct catccgccag caagtcaccg agaacacccg caacaccgcc 360

atcgcccgcc tggagggcct gggccgtggc taccgctcct accagcaagc cctggagacc 420

tggctcgaca accgcaacga cgcccgctcc cgctccatca tcctggagcg ctacgtcgcc 480

ctggaactgg acatcaccac tgccatccca ctcttccgca tcaggaacga ggaggtgcct 540

ctgctgatgg tgtacgccca ggctgcgaac ctgcacctgc tgctgctgcg cgacgcaagc 600

ctgtttggct ccgagtgggg tatggcaagc tccgacgtca accagtacta ccaggagcag 660

atccgctaca ccgaggagta cagcaaccac tgcgtccagt ggtacaacac cggtctgaac 720

aatctcagag ggaccaacgc tgagagctgg ctgcgctaca accagttccg gcgggatctg 780

accctaggtg tcctggatct ggtcgctctg ttcccgagct acgataccag gacgtaccct 840

atcaacacct ctgctcagct taccagggag atctacactg atcctatcgg taggactaac 900

gctcctagtg gtttcgccag cactaactgg ttcaacaaca acgcgcctag tttctctgcc 960

atcgaggcgg cgatcttccg gcctcctcac ctcctcgact tcccggagca gcttactatc 1020

tactctgcgt cttcgcggtg gtcttcgact cagcacatga actactgggt tggtcaccgg 1080

cttaacttcc gcccgattgg aggaactctt aacaccagta cgcaaggtct tacgaacaac 1140

acttccatca acccggttac gttgcagttc acgtctcggg acgtttaccg gacggagtcg 1200

aatgctggga cgaacatcct gttcacgaca ccggtgaatg gtgttccgtg ggcacgtttc 1260

aacttcatca acccgcagaa catctacgag cgtggagcaa cgacatactc gcaaccatac 1320

caaggcgttg gcatccaact gtttgactcg gagacggaac tgccaccaga gacgacagaa 1380

cgtccgaatt acgagtcata ctcacacaga ctatcacaca ttggactcat tatcggaaac 1440

acactgagag caccagtgta ctcatggaca catcggtcag cagatcgtac gaacaccatc 1500

ggacccaatc ggatcaacca gatcccgctc gtgaagggct tccgcgtgtg gggcggcacc 1560

tccgtcatca ccggtccggg cttcaccggc ggcgacatcc tccgccgcaa caccttcggc 1620

gacttcgtgt cactccaagt gaacatcaac agcccgatca cccagcgcta tcgcctccgc 1680

ttccgctacg cctcctcccg cgacgctaga gtgatcgtgc tcaccggagc ggcgtccaca 1740

ggcgtaggcg gccaagtgtc tgtgaacatg ccgctccaga agactatgga gattggtgag 1800

aacctcacct ctcgcacctt ccgctacacc gacttctcca atccgttctc cttcagagcc 1860

aacccagaca tcatcggcat ctccgagcag cctctctttg gcgctggctc catctcctcc 1920

ggcgagctgt acatcgacaa gattgagatc atccttgccg acgccacctt cgaagctgag 1980

tccgatctcg agcgcgccca gaaggccgtg aacgccctct tcactagcac taaccagctc 2040

ggcctcaaga ctaacgtgac cgactaccac attgaccaag tgagcaacct agtgacctac 2100

cttagcgacg agttctgcct tgacgagaag cgtgagctga gcgagaaggt gaagcacgcc 2160

aagcgcctct ccgacgagcg caacctcctc caggactcca acttcaagga catcaaccgc 2220

cagcccgagc gcggctgggg cggtagcacc ggcatcacca tccagggcgg tgacgatgtg 2280

ttcaaggaga actacgtgac cctctccggc accttcgacg agtgctaccc gacctacctc 2340

taccagaaga tcgacgagtc caagctcaag gcgttcaccc gctaccagct tcgcggctac 2400

atcgaggact cccaggatct ggagatctac ctcatccgct acaacgccaa gcacgagacc 2460

gtgaacgtgc ccggcaccgg ctccctctgg ccgctctccg cccagagccc tatcggcaag 2520

tgcggcgagc ccaaccgctg cgcgcctcac ctggagtgga accctgacct cgactgctcc 2580

tgccgcgacg gcgagaagtg cgcccaccat agccaccact tctctctcga catcgacgtg 2640

ggctgcaccg acctcaacga ggatctgggc gtgtgggtga tcttcaagat caagacccag 2700

gacggccacg ccaggctggg caacctggag ttcctggagg agaagcctct ggtgggtgag 2760

gccctggcca gggtcaagag ggctgagaag aaatggaggg acaagaggga gaagctggag 2820

tgggagacca acatcgtgta caaggaggct aaggagtccg tggacgctct gttcgtcaac 2880

tctcagtacg atcagctcca ggctgacacc aacatcgcta tgatccacgc tgcggataag 2940

agggtccact ctatcaggga ggcttacctg cctgagcttt ctgtcatccc tggtgtcaac 3000

gcggcaatct tcgaggaact tgagggccgc atcttcactg cgttctcgct ttacgatgcg 3060

cggaacgtca ttaagaacgg tgacttcaac aatggtcttt cgtgctggaa cgtcaagggt 3120

catgtcgatg tcgaggaaca gaacaaccag cggtcggtcc ttgtcgttcc cgagtgggag 3180

gccgaggtct cgcaagaggt ccgggtctgc cctgggcgcg ggtacattct tcgtgtcact 3240

gcgtacaagg agggctacgg cgagggctgc gttactattc atgagattga gaacaatacg 3300

gatgagctta agtttagtaa ctgtgttgag gaggagatct acccgaacaa tacggttacg 3360

tgcaatgatt acacggtgaa ccaggaggaa tacggcggag catacacctc acgtaataga 3420

gggtacaatg aggcaccgtc agttccggca gattatgcct cagtttatga ggagaagtcc 3480

tacacggatg gaagacgcga gaatccatgt gagtttaata gaggataccg agactacaca 3540

ccactcccag ttggatacgt tacaaaggag ttggaatact tcccagaaac agataaagtt 3600

tggatagaga tcggagaaac agaaggaacc ttcatcgtgg acagtgtaga actgctgctg 3660

atggaagaat ga 3672

<210> 7

<211> 1223

<212> PRT

<213> 人工的

<220>

<223> 嵌合蛋白质TIC860的氨基酸序列。

<400> 7

Met Thr Ser Asn Arg Lys Asn Glu Asn Glu Ile Ile Asn Ala Leu Ser

1 5 10 15

Ile Pro Thr Val Ser Asn Pro Ser Thr Gln Met Asn Leu Ser Pro Asp

20 25 30

Ala Arg Ile Glu Asp Ser Leu Cys Val Ala Glu Val Asn Asn Ile Asp

35 40 45

Pro Phe Val Ser Ala Ser Thr Val Gln Thr Gly Ile Asn Ile Ala Gly

50 55 60

Arg Ile Leu Gly Val Leu Gly Val Pro Phe Ala Gly Gln Leu Ala Ser

65 70 75 80

Phe Tyr Ser Phe Leu Val Gly Glu Leu Trp Pro Ser Gly Arg Asp Pro

85 90 95

Trp Glu Ile Phe Leu Glu His Val Glu Gln Leu Ile Arg Gln Gln Val

100 105 110

Thr Glu Asn Thr Arg Asn Thr Ala Ile Ala Arg Leu Glu Gly Leu Gly

115 120 125

Arg Gly Tyr Arg Ser Tyr Gln Gln Ala Leu Glu Thr Trp Leu Asp Asn

130 135 140

Arg Asn Asp Ala Arg Ser Arg Ser Ile Ile Leu Glu Arg Tyr Val Ala

145 150 155 160

Leu Glu Leu Asp Ile Thr Thr Ala Ile Pro Leu Phe Arg Ile Arg Asn

165 170 175

Glu Glu Val Pro Leu Leu Met Val Tyr Ala Gln Ala Ala Asn Leu His

180 185 190

Leu Leu Leu Leu Arg Asp Ala Ser Leu Phe Gly Ser Glu Trp Gly Met

195 200 205

Ala Ser Ser Asp Val Asn Gln Tyr Tyr Gln Glu Gln Ile Arg Tyr Thr

210 215 220

Glu Glu Tyr Ser Asn His Cys Val Gln Trp Tyr Asn Thr Gly Leu Asn

225 230 235 240

Asn Leu Arg Gly Thr Asn Ala Glu Ser Trp Leu Arg Tyr Asn Gln Phe

245 250 255

Arg Arg Asp Leu Thr Leu Gly Val Leu Asp Leu Val Ala Leu Phe Pro

260 265 270

Ser Tyr Asp Thr Arg Thr Tyr Pro Ile Asn Thr Ser Ala Gln Leu Thr

275 280 285

Arg Glu Ile Tyr Thr Asp Pro Ile Gly Arg Thr Asn Ala Pro Ser Gly

290 295 300

Phe Ala Ser Thr Asn Trp Phe Asn Asn Asn Ala Pro Ser Phe Ser Ala

305 310 315 320

Ile Glu Ala Ala Ile Phe Arg Pro Pro His Leu Leu Asp Phe Pro Glu

325 330 335

Gln Leu Thr Ile Tyr Ser Ala Ser Ser Arg Trp Ser Ser Thr Gln His

340 345 350

Met Asn Tyr Trp Val Gly His Arg Leu Asn Phe Arg Pro Ile Gly Gly

355 360 365

Thr Leu Asn Thr Ser Thr Gln Gly Leu Thr Asn Asn Thr Ser Ile Asn

370 375 380

Pro Val Thr Leu Gln Phe Thr Ser Arg Asp Val Tyr Arg Thr Glu Ser

385 390 395 400

Asn Ala Gly Thr Asn Ile Leu Phe Thr Thr Pro Val Asn Gly Val Pro

405 410 415

Trp Ala Arg Phe Asn Phe Ile Asn Pro Gln Asn Ile Tyr Glu Arg Gly

420 425 430

Ala Thr Thr Tyr Ser Gln Pro Tyr Gln Gly Val Gly Ile Gln Leu Phe

435 440 445

Asp Ser Glu Thr Glu Leu Pro Pro Glu Thr Thr Glu Arg Pro Asn Tyr

450 455 460

Glu Ser Tyr Ser His Arg Leu Ser His Ile Gly Leu Ile Ile Gly Asn

465 470 475 480

Thr Leu Arg Ala Pro Val Tyr Ser Trp Thr His Arg Ser Ala Asp Arg

485 490 495

Thr Asn Thr Ile Gly Pro Asn Arg Ile Asn Gln Ile Pro Leu Val Lys

500 505 510

Gly Phe Arg Val Trp Gly Gly Thr Ser Val Ile Thr Gly Pro Gly Phe

515 520 525

Thr Gly Gly Asp Ile Leu Arg Arg Asn Thr Phe Gly Asp Phe Val Ser

530 535 540

Leu Gln Val Asn Ile Asn Ser Pro Ile Thr Gln Arg Tyr Arg Leu Arg

545 550 555 560

Phe Arg Tyr Ala Ser Ser Arg Asp Ala Arg Val Ile Val Leu Thr Gly

565 570 575

Ala Ala Ser Thr Gly Val Gly Gly Gln Val Ser Val Asn Met Pro Leu

580 585 590

Gln Lys Thr Met Glu Ile Gly Glu Asn Leu Thr Ser Arg Thr Phe Arg

595 600 605

Tyr Thr Asp Phe Ser Asn Pro Phe Ser Phe Arg Ala Asn Pro Asp Ile

610 615 620

Ile Gly Ile Ser Glu Gln Pro Leu Phe Gly Ala Gly Ser Ile Ser Ser

625 630 635 640

Gly Glu Leu Tyr Ile Asp Lys Ile Glu Ile Ile Leu Ala Asp Ala Thr

645 650 655

Phe Glu Ala Glu Ser Asp Leu Glu Arg Ala Gln Lys Ala Val Asn Ala

660 665 670

Leu Phe Thr Ser Thr Asn Gln Leu Gly Leu Lys Thr Asn Val Thr Asp

675 680 685

Tyr His Ile Asp Gln Val Ser Asn Leu Val Thr Tyr Leu Ser Asp Glu

690 695 700

Phe Cys Leu Asp Glu Lys Arg Glu Leu Ser Glu Lys Val Lys His Ala

705 710 715 720

Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Ser Asn Phe Lys

725 730 735

Asp Ile Asn Arg Gln Pro Glu Arg Gly Trp Gly Gly Ser Thr Gly Ile

740 745 750

Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val Thr Leu

755 760 765

Ser Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln Lys Ile

770 775 780

Asp Glu Ser Lys Leu Lys Ala Phe Thr Arg Tyr Gln Leu Arg Gly Tyr

785 790 795 800

Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr Asn Ala

805 810 815

Lys His Glu Thr Val Asn Val Pro Gly Thr Gly Ser Leu Trp Pro Leu

820 825 830

Ser Ala Gln Ser Pro Ile Gly Lys Cys Gly Glu Pro Asn Arg Cys Ala

835 840 845

Pro His Leu Glu Trp Asn Pro Asp Leu Asp Cys Ser Cys Arg Asp Gly

850 855 860

Glu Lys Cys Ala His His Ser His His Phe Ser Leu Asp Ile Asp Val

865 870 875 880

Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Val Ile Phe Lys

885 890 895

Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn Leu Glu Phe Leu

900 905 910

Glu Glu Lys Pro Leu Val Gly Glu Ala Leu Ala Arg Val Lys Arg Ala

915 920 925

Glu Lys Lys Trp Arg Asp Lys Arg Glu Lys Leu Glu Trp Glu Thr Asn

930 935 940

Ile Val Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala Leu Phe Val Asn

945 950 955 960

Ser Gln Tyr Asp Gln Leu Gln Ala Asp Thr Asn Ile Ala Met Ile His

965 970 975

Ala Ala Asp Lys Arg Val His Ser Ile Arg Glu Ala Tyr Leu Pro Glu

980 985 990

Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile Phe Glu Glu Leu Glu

995 1000 1005

Gly Arg Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala Arg Asn Val

1010 1015 1020

Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Ser Cys Trp Asn Val

1025 1030 1035

Lys Gly His Val Asp Val Glu Glu Gln Asn Asn Gln Arg Ser Val

1040 1045 1050

Leu Val Val Pro Glu Trp Glu Ala Glu Val Ser Gln Glu Val Arg

1055 1060 1065

Val Cys Pro Gly Arg Gly Tyr Ile Leu Arg Val Thr Ala Tyr Lys

1070 1075 1080

Glu Gly Tyr Gly Glu Gly Cys Val Thr Ile His Glu Ile Glu Asn

1085 1090 1095

Asn Thr Asp Glu Leu Lys Phe Ser Asn Cys Val Glu Glu Glu Ile

1100 1105 1110

Tyr Pro Asn Asn Thr Val Thr Cys Asn Asp Tyr Thr Val Asn Gln

1115 1120 1125

Glu Glu Tyr Gly Gly Ala Tyr Thr Ser Arg Asn Arg Gly Tyr Asn

1130 1135 1140

Glu Ala Pro Ser Val Pro Ala Asp Tyr Ala Ser Val Tyr Glu Glu

1145 1150 1155

Lys Ser Tyr Thr Asp Gly Arg Arg Glu Asn Pro Cys Glu Phe Asn

1160 1165 1170

Arg Gly Tyr Arg Asp Tyr Thr Pro Leu Pro Val Gly Tyr Val Thr

1175 1180 1185

Lys Glu Leu Glu Tyr Phe Pro Glu Thr Asp Lys Val Trp Ile Glu

1190 1195 1200

Ile Gly Glu Thr Glu Gly Thr Phe Ile Val Asp Ser Val Glu Leu

1205 1210 1215

Leu Leu Met Glu Glu

1220

<210> 8

<211> 3564

<212> DNA

<213> 人工的

<220>

<223> 用于在细菌细胞中表达的编码TIC867的重组核苷酸序列。

<400> 8

atgacttcaa ataggaaaaa tgagaatgaa attataaatg ctttatcgat tccagctgta 60

tcgaatcatt ccgcacaaat gaatctatca accgatgctc gtattgagga tagcttgtgt 120

atagccgagg ggaacaatat cgatccattt gttagcgcat caacagtcca aacgggtatt 180

aacatagctg gtagaatact aggtgtatta ggcgtaccgt ttgctggaca aatagctagt 240

ttttatagtt ttcttgttgg tgaattatgg ccccgcggca gagatccttg ggaaattttc 300

ctagaacatg tcgaacaact tataagacaa caagtaacag aaaatactag ggatacggct 360

cttgctcgat tacaaggttt aggaaattcc tttagagcct atcaacagtc acttgaagat 420

tggctagaaa accgtgatga tgcaagaacg agaagtgttc tttataccca atatatagcc 480

ttagaacttg attttcttaa tgcgatgccg cttttcgcaa ttagaaacca agaagttcca 540

ttattaatgg tatatgctca agctgcaaat ttacacctat tattattgag agatgcctct 600

ctttttggta gtgaatttgg gcttacatcc caagaaattc aacgttatta tgagcgccaa 660

gtggaaaaaa cgagagaata ttctgattat tgcgcaagat ggtataatac gggtttaaat 720

aatttgagag ggacaaatgc tgaaagttgg ttgcgatata atcaattccg tagagactta 780

acgctaggag tattagatct agtggcacta ttcccaagct atgacacgcg tgtttatcca 840

atgaatacca gtgctcaatt aacaagagaa atttatacag atccaattgg gagaacaaat 900

gcaccttcag gatttgcaag tacgaattgg tttaataata atgcaccatc gttttctgcc 960

atagaggctg ccgttattag gcctccgcat ctacttgatt ttccagaaca gcttacaatt 1020

ttcagcgtat taagtcgatg gagtaatact caatatatga attactgggt gggacataga 1080

cttgaatcgc gaacaataag ggggtcatta agtacctcga cacacggaaa taccaatact 1140

tctattaatc ctgtaacatt acagttcaca tctcgagacg tttatagaac agaatcattt 1200

gcagggataa atatacttct aactactcct gtgaatggag taccttgggc tagatttaat 1260

tggagaaatc ccctgaattc tcttagaggt agccttctct atactatagg gtatactgga 1320

gtggggacac aactatttga ttcagaaact gaattaccac cagaaacaac agaacgacca 1380

aattatgaat cttacagtca tagattatct aatataagac taatatcagg aaacactttg 1440

agagcaccag tatattcttg gacgcaccgt agtgcagatc gtacaaatac cattagttca 1500

gatagcatta cacaaatacc attggtaaag gcgcataccc tccaatcggg taccactgta 1560

gtaaaagggc cagggtttac aggaggggat atcctccgtc gaacaagtgg aggaccattt 1620

gcttttagta atgttaatct agattttaac ttgtcacaaa ggtatcgtgc tagaattcgt 1680

tatgcctcta ctactaacct aagaatttac gtaacggttg caggtgaacg aatttttgct 1740

ggtcaatttg acaaaactat ggatgctggt gccccattaa cattccaatc ttttagttac 1800

gcaactatta atacagcttt tacattccca gaaagatcga gcagcttgac tgtaggtgcc 1860

gatacgttta gttcaggtaa tgaagtttat gtagatagat ttgaattaat cccagttact 1920

gcaaccttcg aggcagaatc tgatttagaa agagcacaaa aggcggtgaa tgagctgttt 1980

acttcttcca atcaaatcgg gttaaaaaca gatgtgacgg attatcatat tgatcaagta 2040

tccaatttag ttgagtgttt atctgatgaa ttttgtctgg atgaaaaaaa agaattgtcc 2100

gagaaagtca aacatgcgaa gcgacttagt gatgagcgga atttacttca agatccaaac 2160

tttagaggga tcaatagaca actagaccgt ggctggagag gaagtacgga tattaccatc 2220

caaggaggcg atgacgtatt caaagagaat tacgttacgc tattgggtac ctttgatgag 2280

tgctatccaa cgtatttata tcaaaaaata gatgagtcga aattaaaagc ctatacccgt 2340

taccaattaa gagggtatat cgaagatagt caagacttag aaatctattt aattcgctac 2400

aatgccaaac acgaaacagt aaatgtgcca ggtacgggtt ccttatggcc gctttcagcc 2460

ccaagtccaa tcggaaaatg tgcccatcat tcccatcatt tctccttgga cattgatgtt 2520

ggatgtacag acttaaatga ggacttaggt gtatgggtga tattcaagat taagacgcaa 2580

gatggccatg caagactagg aaatctagaa tttctcgaag agaaaccatt agtaggagaa 2640

gcactagctc gtgtgaaaag agcggagaaa aaatggagag acaaacgtga aaaattggaa 2700

tgggaaacaa atattgttta taaagaggca aaagaatctg tagatgcttt atttgtaaac 2760

tctcaatatg atagattaca agcggatacc aacatcgcga tgattcatgc ggcagataaa 2820

cgcgttcata gcattcgaga agcttatctg cctgagctgt ctgtgattcc gggtgtcaat 2880

gcggctattt ttgaagaatt agaagggcgt attttcactg cattctccct atatgatgcg 2940

agaaatgtca ttaaaaatgg tgattttaat aatggcttat cctgctggaa cgtgaaaggg 3000

catgtagatg tagaagaaca aaacaaccac cgttcggtcc ttgttgttcc ggaatgggaa 3060

gcagaagtgt cacaagaagt tcgtgtctgt ccgggtcgtg gctatatcct tcgtgtcaca 3120

gcgtacaagg agggatatgg agaaggttgc gtaaccattc atgagatcga gaacaataca 3180

gacgaactga agtttagcaa ctgtgtagaa gaggaagtat atccaaacaa cacggtaacg 3240

tgtaatgatt atactgcgac tcaagaagaa tatgagggta cgtacacttc tcgtaatcga 3300

ggatatgacg gagcctatga aagcaattct tctgtaccag ctgattatgc atcagcctat 3360

gaagaaaaag catatacaga tggacgaaga gacaatcctt gtgaatctaa cagaggatat 3420

ggggattaca caccactacc agctggctat gtgacaaaag aattagagta cttcccagaa 3480

accgataagg tatggattga gatcggagaa acggaaggaa cattcatcgt ggacagcgtg 3540

gaattacttc ttatggagga atag 3564

<210> 9

<211> 3564

<212> DNA

<213> 人工的

<220>

<223> 设计用于在植物细胞中表达的编码TIC867的合成核苷酸序列。

<400> 9

atgaccagca accgaaagaa cgagaacgag atcatcaacg ccctgtccat accggccgtg 60

tcaaaccact ccgcccagat gaacctctcc accgacgcga ggatcgagga ctccctctgc 120

atcgccgagg gcaacaacat cgacccgttc gtgtctgcaa gcacggtcca gaccggcatc 180

aacatcgcgg gccgcatcct gggcgtgctc ggcgtgccct tcgcgggtca aatcgcctct 240

ttctactcat tcctcgtggg cgagctgtgg ccgcgcggac gtgacccgtg ggaaatcttc 300

ctggagcacg ttgagcagct catccggcag caagtgaccg agaacaccag ggacaccgca 360

ctggcacggc tccagggcct tggcaacagc ttccgcgcct accagcagtc gctggaggac 420

tggctggaga accgagacga cgccagaacc cgctcagttc tgtacacaca gtacatcgcc 480

ctagagctgg acttcctcaa cgctatgccg ctcttcgcca tccgtaacca ggaagtaccg 540

cttctgatgg tgtacgcaca agcagcgaac ctccatctgc tcctgctgcg agacgcatct 600

ctgttcggca gtgagttcgg gctgacgagc caggagatcc agcgctacta cgagcgccaa 660

gtggagaaga ctcgtgagta cagcgactac tgcgcgcgct ggtacaacac gggcttgaac 720

aaccttcgcg ggacaaacgc cgaatcctgg cttcgctaca accagttccg ccgcgacctc 780

acgctgggtg tgctggacct ggtcgcgctc ttcccgtcct acgacacacg ggtgtaccca 840

atgaacacga gcgcacagct cacccgtgag atctacacag atcccatcgg ccgcaccaac 900

gctcccagtg gcttcgcaag cacgaattgg ttcaacaata acgctccttc tttctctgcc 960

atcgaggccg ctgtcatcag accgccgcac ttactcgatt tcccggagca gctcactatc 1020

ttctctgtgt tgtcccggtg gtcgaacacg cagtacatga actactgggt gggccacagg 1080

ctagagagcc ggaccatccg tggcagtctc tcaacctcga cccacggcaa cacgaacacg 1140

agcatcaacc ctgtcactct ccagtttaca tctagggacg tttacaggac agagtcgttc 1200

gctggcatta acattctgtt gaccactccg gtgaacggcg tcccttgggc ccgcttcaac 1260

tggaggaatc ctctgaactc actgcgcggc agccttctct acactatcgg ctacaccggc 1320

gttgggacgc aactcttcga ctcggagacc gagctgccgc ccgagaccac cgagcggcct 1380

aactacgaga gttattcaca caggctctcc aacatccgct tgatttctgg gaacaccttg 1440

cgggctccgg tgtactcctg gacgcaccgc agcgccgaca gaactaatac catcagctcc 1500

gactcgatca cccagatccc gctggtgaag gctcacacgc ttcagtcggg caccacagtc 1560

gtcaagggcc ctggcttcac cggcggcgac atcctgcgtc gcacatctgg cggacccttc 1620

gccttcagca acgtgaactt ggacttcaat ttgtcacagc ggtatcgtgc cagaatccgg 1680

tacgccagca ctacgaacct gcgaatctat gttactgtgg cgggcgagcg gatcttcgcc 1740

gggcaattcg acaagacgat ggacgcggga gcacctctga cattccagtc attctcttac 1800

gccacgatca acacggcatt cacgtttccg gagcgttcca gtagcctgac cgtgggcgct 1860

gataccttca gtagcgggaa cgaggtgtac gttgaccgtt tcgagctgat cccggtcacc 1920

gccaccttcg aagctgagtc ggacctggag cgtgcacaga aggcagtcaa cgagctgttc 1980

acctctagca accagatcgg cctcaagacc gacgtcacag actaccacat cgaccaagtg 2040

tccaacctgg tcgagtgcct tagcgacgag ttctgcctag acgagaagaa ggagctgtcg 2100

gagaaggtca aacacgccaa gcgtctgagc gatgagcgca acctgctcca agaccctaac 2160

ttccgtggca tcaacaggca gcttgaccgt ggctggcgcg gctcgacgga catcacgatc 2220

cagggtggcg acgacgtatt caaggagaat tacgtgacct tgcttgggac gtttgacgag 2280

tgctatccca cctacctcta ccagaagatt gatgaatcga aattgaaggc gtacacgaga 2340

taccagctcc gtggctacat cgaggacagc caggacttgg agatctacct catacgctac 2400

aacgctaaac atgagaccgt gaacgtccct gggacgggca gtctgtggcc actctctgct 2460

cctagcccta tcggcaagtg cgctcaccac tcgcaccact tcagccttga catcgacgtg 2520

ggatgtactg acctcaacga agacctgggc gtctgggtta tcttcaagat caagacccag 2580

gacggccacg cccgactcgg caacctggag ttcctggagg agaaaccact ggtgggcgag 2640

gcgctcgccc gcgtgaagcg tgccgagaag aagtggcggg acaagaggga gaagctagaa 2700

tgggagacga acatcgtgta caaggaggcc aaggaaagcg tcgatgccct gttcgtgaac 2760

tcacagtacg accgtctcca ggcggacacg aacatcgcca tgatccacgc ggctgacaag 2820

cgcgtccact ccatccgcga ggcgtactta ccggagctgt cggtgatccc aggcgtaaac 2880

gcggcgatct tcgaggagct agagggacgc atcttcacag cgttcagcct gtacgacgca 2940

cgcaacgtca tcaagaacgg cgatttcaac aacggactgt cctgctggaa cgtgaagggc 3000

cacgtcgatg tcgaggaaca gaacaaccac cgctctgtcc tggtggtccc agagtgggag 3060

gccgaggtct cccaggaggt ccgcgtgtgc cctgggcgtg gctacatcct ccgtgtgaca 3120

gcctacaagg agggctacgg tgagggctgc gtcaccattc acgagatcga gaacaacact 3180

gacgaactca agttctcgaa ttgcgtggag gaggaggtgt acccgaacaa tacggtgacg 3240

tgcaacgact acacggcaac ccaagaggag tacgagggca cctacaccag taggaaccgt 3300

ggctacgacg gtgcctacga gtcgaactcc agcgtccctg cggactacgc cagcgcgtac 3360

gaggagaagg cttacaccga cggacgccgg gacaacccat gcgagagcaa ccgtggctac 3420

ggcgactaca ctcctctccc ggccggatac gtcacaaagg agctggagta tttcccagag 3480

acggacaagg tgtggatcga aatcggagag acagagggaa ccttcatcgt ggacagcgtg 3540

gagctgctcc tcatggagga gtga 3564

<210> 10

<211> 1187

<212> PRT

<213> 人工的

<220>

<223> 嵌合蛋白质TIC867的氨基酸序列。

<400> 10

Met Thr Ser Asn Arg Lys Asn Glu Asn Glu Ile Ile Asn Ala Leu Ser

1 5 10 15

Ile Pro Ala Val Ser Asn His Ser Ala Gln Met Asn Leu Ser Thr Asp

20 25 30

Ala Arg Ile Glu Asp Ser Leu Cys Ile Ala Glu Gly Asn Asn Ile Asp

35 40 45

Pro Phe Val Ser Ala Ser Thr Val Gln Thr Gly Ile Asn Ile Ala Gly

50 55 60

Arg Ile Leu Gly Val Leu Gly Val Pro Phe Ala Gly Gln Ile Ala Ser

65 70 75 80

Phe Tyr Ser Phe Leu Val Gly Glu Leu Trp Pro Arg Gly Arg Asp Pro

85 90 95

Trp Glu Ile Phe Leu Glu His Val Glu Gln Leu Ile Arg Gln Gln Val

100 105 110

Thr Glu Asn Thr Arg Asp Thr Ala Leu Ala Arg Leu Gln Gly Leu Gly

115 120 125

Asn Ser Phe Arg Ala Tyr Gln Gln Ser Leu Glu Asp Trp Leu Glu Asn

130 135 140

Arg Asp Asp Ala Arg Thr Arg Ser Val Leu Tyr Thr Gln Tyr Ile Ala

145 150 155 160

Leu Glu Leu Asp Phe Leu Asn Ala Met Pro Leu Phe Ala Ile Arg Asn

165 170 175

Gln Glu Val Pro Leu Leu Met Val Tyr Ala Gln Ala Ala Asn Leu His

180 185 190

Leu Leu Leu Leu Arg Asp Ala Ser Leu Phe Gly Ser Glu Phe Gly Leu

195 200 205

Thr Ser Gln Glu Ile Gln Arg Tyr Tyr Glu Arg Gln Val Glu Lys Thr

210 215 220

Arg Glu Tyr Ser Asp Tyr Cys Ala Arg Trp Tyr Asn Thr Gly Leu Asn

225 230 235 240

Asn Leu Arg Gly Thr Asn Ala Glu Ser Trp Leu Arg Tyr Asn Gln Phe

245 250 255

Arg Arg Asp Leu Thr Leu Gly Val Leu Asp Leu Val Ala Leu Phe Pro

260 265 270

Ser Tyr Asp Thr Arg Val Tyr Pro Met Asn Thr Ser Ala Gln Leu Thr

275 280 285

Arg Glu Ile Tyr Thr Asp Pro Ile Gly Arg Thr Asn Ala Pro Ser Gly

290 295 300

Phe Ala Ser Thr Asn Trp Phe Asn Asn Asn Ala Pro Ser Phe Ser Ala

305 310 315 320

Ile Glu Ala Ala Val Ile Arg Pro Pro His Leu Leu Asp Phe Pro Glu

325 330 335

Gln Leu Thr Ile Phe Ser Val Leu Ser Arg Trp Ser Asn Thr Gln Tyr

340 345 350

Met Asn Tyr Trp Val Gly His Arg Leu Glu Ser Arg Thr Ile Arg Gly

355 360 365

Ser Leu Ser Thr Ser Thr His Gly Asn Thr Asn Thr Ser Ile Asn Pro

370 375 380

Val Thr Leu Gln Phe Thr Ser Arg Asp Val Tyr Arg Thr Glu Ser Phe

385 390 395 400

Ala Gly Ile Asn Ile Leu Leu Thr Thr Pro Val Asn Gly Val Pro Trp

405 410 415

Ala Arg Phe Asn Trp Arg Asn Pro Leu Asn Ser Leu Arg Gly Ser Leu

420 425 430

Leu Tyr Thr Ile Gly Tyr Thr Gly Val Gly Thr Gln Leu Phe Asp Ser

435 440 445

Glu Thr Glu Leu Pro Pro Glu Thr Thr Glu Arg Pro Asn Tyr Glu Ser

450 455 460

Tyr Ser His Arg Leu Ser Asn Ile Arg Leu Ile Ser Gly Asn Thr Leu

465 470 475 480

Arg Ala Pro Val Tyr Ser Trp Thr His Arg Ser Ala Asp Arg Thr Asn

485 490 495

Thr Ile Ser Ser Asp Ser Ile Thr Gln Ile Pro Leu Val Lys Ala His

500 505 510

Thr Leu Gln Ser Gly Thr Thr Val Val Lys Gly Pro Gly Phe Thr Gly

515 520 525

Gly Asp Ile Leu Arg Arg Thr Ser Gly Gly Pro Phe Ala Phe Ser Asn

530 535 540

Val Asn Leu Asp Phe Asn Leu Ser Gln Arg Tyr Arg Ala Arg Ile Arg

545 550 555 560

Tyr Ala Ser Thr Thr Asn Leu Arg Ile Tyr Val Thr Val Ala Gly Glu

565 570 575

Arg Ile Phe Ala Gly Gln Phe Asp Lys Thr Met Asp Ala Gly Ala Pro

580 585 590

Leu Thr Phe Gln Ser Phe Ser Tyr Ala Thr Ile Asn Thr Ala Phe Thr

595 600 605

Phe Pro Glu Arg Ser Ser Ser Leu Thr Val Gly Ala Asp Thr Phe Ser

610 615 620

Ser Gly Asn Glu Val Tyr Val Asp Arg Phe Glu Leu Ile Pro Val Thr

625 630 635 640

Ala Thr Phe Glu Ala Glu Ser Asp Leu Glu Arg Ala Gln Lys Ala Val

645 650 655

Asn Glu Leu Phe Thr Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp Val

660 665 670

Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Glu Cys Leu Ser

675 680 685

Asp Glu Phe Cys Leu Asp Glu Lys Lys Glu Leu Ser Glu Lys Val Lys

690 695 700

His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn

705 710 715 720

Phe Arg Gly Ile Asn Arg Gln Leu Asp Arg Gly Trp Arg Gly Ser Thr

725 730 735

Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val

740 745 750

Thr Leu Leu Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln

755 760 765

Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg

770 775 780

Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr

785 790 795 800

Asn Ala Lys His Glu Thr Val Asn Val Pro Gly Thr Gly Ser Leu Trp

805 810 815

Pro Leu Ser Ala Pro Ser Pro Ile Gly Lys Cys Ala His His Ser His

820 825 830

His Phe Ser Leu Asp Ile Asp Val Gly Cys Thr Asp Leu Asn Glu Asp

835 840 845

Leu Gly Val Trp Val Ile Phe Lys Ile Lys Thr Gln Asp Gly His Ala

850 855 860

Arg Leu Gly Asn Leu Glu Phe Leu Glu Glu Lys Pro Leu Val Gly Glu

865 870 875 880

Ala Leu Ala Arg Val Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg

885 890 895

Glu Lys Leu Glu Trp Glu Thr Asn Ile Val Tyr Lys Glu Ala Lys Glu

900 905 910

Ser Val Asp Ala Leu Phe Val Asn Ser Gln Tyr Asp Arg Leu Gln Ala

915 920 925

Asp Thr Asn Ile Ala Met Ile His Ala Ala Asp Lys Arg Val His Ser

930 935 940

Ile Arg Glu Ala Tyr Leu Pro Glu Leu Ser Val Ile Pro Gly Val Asn

945 950 955 960

Ala Ala Ile Phe Glu Glu Leu Glu Gly Arg Ile Phe Thr Ala Phe Ser

965 970 975

Leu Tyr Asp Ala Arg Asn Val Ile Lys Asn Gly Asp Phe Asn Asn Gly

980 985 990

Leu Ser Cys Trp Asn Val Lys Gly His Val Asp Val Glu Glu Gln Asn

995 1000 1005

Asn His Arg Ser Val Leu Val Val Pro Glu Trp Glu Ala Glu Val

1010 1015 1020

Ser Gln Glu Val Arg Val Cys Pro Gly Arg Gly Tyr Ile Leu Arg

1025 1030 1035

Val Thr Ala Tyr Lys Glu Gly Tyr Gly Glu Gly Cys Val Thr Ile

1040 1045 1050

His Glu Ile Glu Asn Asn Thr Asp Glu Leu Lys Phe Ser Asn Cys

1055 1060 1065

Val Glu Glu Glu Val Tyr Pro Asn Asn Thr Val Thr Cys Asn Asp

1070 1075 1080

Tyr Thr Ala Thr Gln Glu Glu Tyr Glu Gly Thr Tyr Thr Ser Arg

1085 1090 1095

Asn Arg Gly Tyr Asp Gly Ala Tyr Glu Ser Asn Ser Ser Val Pro

1100 1105 1110

Ala Asp Tyr Ala Ser Ala Tyr Glu Glu Lys Ala Tyr Thr Asp Gly

1115 1120 1125

Arg Arg Asp Asn Pro Cys Glu Ser Asn Arg Gly Tyr Gly Asp Tyr

1130 1135 1140

Thr Pro Leu Pro Ala Gly Tyr Val Thr Lys Glu Leu Glu Tyr Phe

1145 1150 1155

Pro Glu Thr Asp Lys Val Trp Ile Glu Ile Gly Glu Thr Glu Gly

1160 1165 1170

Thr Phe Ile Val Asp Ser Val Glu Leu Leu Leu Met Glu Glu

1175 1180 1185

<210> 11

<211> 3642

<212> DNA

<213> 人工的

<220>

<223> 用于在细菌细胞中表达的编码TIC867_20的重组核苷酸序列。

<400> 11

atgacttcaa ataggaaaaa tgagaatgaa attataaatg ctttatcgat tccagctgta 60

tcgaatcatt ccgcacaaat gaatctatca accgatgctc gtattgagga tagcttgtgt 120

atagccgagg ggaacaatat cgatccattt gttagcgcat caacagtcca aacgggtatt 180

aacatagctg gtagaatact aggtgtatta ggcgtaccgt ttgctggaca aatagctagt 240

ttttatagtt ttcttgttgg tgaattatgg ccccgcggca gagatccttg ggaaattttc 300

ctagaacatg tcgaacaact tataagacaa caagtaacag aaaatactag ggatacggct 360

cttgctcgat tacaaggttt aggaaattcc tttagagcct atcaacagtc acttgaagat 420

tggctagaaa accgtgatga tgcaagaacg agaagtgttc tttataccca atatatagcc 480

ttagaacttg attttcttaa tgcgatgccg cttttcgcaa ttagaaacca agaagttcca 540

ttattaatgg tatatgctca agctgcaaat ttacacctat tattattgag agatgcctct 600

ctttttggta gtgaatttgg gcttacatcc caagaaattc aacgttatta tgagcgccaa 660

gtggaaaaaa cgagagaata ttctgattat tgcgcaagat ggtataatac gggtttaaat 720

aatttgagag ggacaaatgc tgaaagttgg ttgcgatata atcaattccg tagagactta 780

acgctaggag tattagatct agtggcacta ttcccaagct atgacacgcg tgtttatcca 840

atgaatacca gtgctcaatt aacaagagaa atttatacag atccaattgg gagaacaaat 900

gcaccttcag gatttgcaag tacgaattgg tttaataata atgcaccatc gttttctgcc 960

atagaggctg ccgttattag gcctccgcat ctacttgatt ttccagaaca gcttacaatt 1020

ttcagcgtat taagtcgatg gagtaatact caatatatga attactgggt gggacataga 1080

cttgaatcgc gaacaataag ggggtcatta agtacctcga cacacggaaa taccaatact 1140

tctattaatc ctgtaacatt acagttcaca tctcgagacg tttatagaac agaatcattt 1200

gcagggataa atatacttct aactactcct gtgaatggag taccttgggc tagatttaat 1260

tggagaaatc ccctgaattc tcttagaggt agccttctct atactatagg gtatactgga 1320

gtggggacac aactatttga ttcagaaact gaattaccac cagaaacaac agaacgacca 1380

aattatgaat cttacagtca tagattatct aatataagac taatatcagg aaacactttg 1440

agagcaccag tatattcttg gacgcaccgt agtgcagatc gtacaaatac cattagttca 1500

gatagcatta cacaaatacc attggtaaag gcgcataccc tccaatcggg taccactgta 1560

gtaaaagggc cagggtttac aggaggggat atcctccgtc gaacaagtgg aggaccattt 1620

gcttttagta atgttaatct agattttaac ttgtcacaaa ggtatcgtgc tagaattcgt 1680

tatgcctcta ctactaacct aagaatttac gtaacggttg caggtgaacg aatttttgct 1740

ggtcaatttg acaaaactat ggatgctggt gccccattaa cattccaatc ttttagttac 1800

gcaactatta atacagcttt tacattccca gaaagatcga gcagcttgac tgtaggtgcc 1860

gatacgttta gttcaggtaa tgaagtttat gtagatagat ttgaattaat cccagttact 1920

gcaacctttg aggcagaata tgatttagaa agagcgcaaa aggtggtgaa tgccctgttt 1980

acgtctacaa accaactagg gctaaaaaca gatgtgacgg attatcatat tgatcaggta 2040

tccaatctag ttgcgtgttt atcggatgaa ttttgtctgg atgaaaagag agaattgtcc 2100

gagaaagtta aacatgcaaa gcgactcagt gatgagcgga atttacttca agatccaaac 2160

ttcagaggga tcaataggca accagaccgt ggctggagag gaagtacgga tattactatc 2220

caaggaggag atgacgtatt caaagagaat tacgttacgc taccgggtac ctttgatgag 2280

tgctatccaa cgtatttata tcaaaaaata gatgagtcga aattaaaagc ctatacccgt 2340

tatcaattaa gagggtatat cgaagatagt caagacttag aaatctattt aattcgttac 2400

aatgcaaaac acgaaatagt aaatgtacca ggtacaggaa gtttatggcc tctttctgta 2460

gaaaatcaaa ttggaccttg tggagaaccg aatcgatgcg cgccacacct tgaatggaat 2520

cctgatttac actgttcctg cagagacggg gaaaaatgtg cacatcattc tcatcatttc 2580

tctttggaca ttgatgttgg atgtacagac ttaaatgagg acttaggtgt atgggtgata 2640

ttcaagatta agacgcaaga tggccacgca cgactaggga atctagagtt tctcgaagag 2700

aaaccattat taggagaagc actagctcgt gtgaaaagag cggagaaaaa atggagagac 2760

aaacgcgaaa cattacaatt ggaaacaact atcgtttata aagaggcaaa agaatctgta 2820

gatgctttat ttgtaaactc tcaatatgat agattacaag cggatacgaa catcgcgatg 2880

attcatgcgg cagataaacg cgttcataga attcgagaag cgtatctgcc ggagctgtct 2940

gtgattccgg gtgtcaatgc ggctattttt gaagaattag aagagcgtat tttcactgca 3000

ttttccctat atgatgcgag aaatattatt aaaaatggcg atttcaataa tggcttatta 3060

tgctggaacg tgaaagggca tgtagaggta gaagaacaaa acaatcaccg ttcagtcctg 3120

gttatcccag aatgggaggc agaagtgtca caagaggttc gtgtctgtcc aggtcgtggc 3180

tatatccttc gtgttacagc gtacaaagag ggatatggag aaggttgcgt aacgatccat 3240

gagatcgaga acaatacaga cgaactgaaa ttcaacaact gtgtagaaga ggaagtatat 3300

ccaaacaaca cggtaacgtg tattaattat actgcgactc aagaagaata tgagggtacg 3360

tacacttctc gtaatcgagg atatgacgaa gcctatggta ataacccttc cgtaccagct 3420

gattatgcgt cagtctatga agaaaaatcg tatacagata gacgaagaga gaatccttgt 3480

gaatctaaca gaggatatgg agattacaca ccactaccag ctggttatgt aacaaaggaa 3540

ttagagtact tcccagagac cgataaggta tggattgaga ttggagaaac agaaggaaca 3600

ttcatcgtgg acagcgtgga attactcctt atggaggaat ag 3642

<210> 12

<211> 3642

<212> DNA

<213> 人工的

<220>

<223> 设计用于在植物细胞中表达的编码TIC867_20的合成核苷酸序列。

<400> 12

atgaccagca accgaaagaa cgagaacgag atcatcaacg ccctgtccat accggccgtg 60

tcaaaccact ccgcccagat gaacctctcc accgacgcga ggatcgagga ctccctctgc 120

atcgccgagg gcaacaacat cgacccgttc gtgtctgcaa gcacggtcca gaccggcatc 180

aacatcgcgg gccgcatcct gggcgtgctc ggcgtgccct tcgcgggtca aatcgcctct 240

ttctactcat tcctcgtggg cgagctgtgg ccgcgcggac gtgacccgtg ggaaatcttc 300

ctggagcacg ttgagcagct catccggcag caagtgaccg agaacaccag ggacaccgca 360

ctggcacggc tccagggcct tggcaacagc ttccgcgcct accagcagtc gctggaggac 420

tggctggaga accgagacga cgccagaacc cgctcagttc tgtacacaca gtacatcgcc 480

ctagagctgg acttcctcaa cgctatgccg ctcttcgcca tccgtaacca ggaagtaccg 540

cttctgatgg tgtacgcaca agcagcgaac ctccatctgc tcctgctgcg agacgcatct 600

ctgttcggca gtgagttcgg gctgacgagc caggagatcc agcgctacta cgagcgccaa 660

gtggagaaga ctcgtgagta cagcgactac tgcgcgcgct ggtacaacac gggcttgaac 720

aaccttcgcg ggacaaacgc cgaatcctgg cttcgctaca accagttccg ccgcgacctc 780

acgctgggtg tgctggacct ggtcgcgctc ttcccgtcct acgacacacg ggtgtaccca 840

atgaacacga gcgcacagct cacccgtgag atctacacag atcccatcgg ccgcaccaac 900

gctcccagtg gcttcgcaag cacgaattgg ttcaacaata acgctccttc tttctctgcc 960

atcgaggccg ctgtcatcag accgccgcac ttactcgatt tcccggagca gctcactatc 1020

ttctctgtgt tgtcccggtg gtcgaacacg cagtacatga actactgggt gggccacagg 1080

ctagagagcc ggaccatccg tggcagtctc tcaacctcga cccacggcaa cacgaacacg 1140

agcatcaacc ctgtcactct ccagtttaca tctagggacg tttacaggac agagtcgttc 1200

gctggcatta acattctgtt gaccactccg gtgaacggcg tcccttgggc ccgcttcaac 1260

tggaggaatc ctctgaactc actgcgcggc agccttctct acactatcgg ctacaccggc 1320

gttgggacgc aactcttcga ctcggagacc gagctgccgc ccgagaccac cgagcggcct 1380

aactacgaga gttattcaca caggctctcc aacatccgct tgatttctgg gaacaccttg 1440

cgggctccgg tgtactcctg gacgcaccgc agcgccgaca gaactaatac catcagctcc 1500

gactcgatca cccagatccc gctggtgaag gctcacacgc ttcagtcggg caccacagtc 1560

gtcaagggcc ctggcttcac cggcggcgac atcctgcgtc gcacatctgg cggacccttc 1620

gccttcagca acgtgaactt ggacttcaat ttgtcacagc ggtatcgtgc cagaatccgg 1680

tacgccagca ctacgaacct gcgaatctat gttactgtgg cgggcgagcg gatcttcgcc 1740

gggcaattcg acaagacgat ggacgcggga gcacctctga cattccagtc attctcttac 1800

gccacgatca acacggcatt cacgtttccg gagcgttcca gtagcctgac cgtgggcgct 1860

gataccttca gtagcgggaa cgaggtgtac gttgaccgtt tcgagctgat cccggtcacc 1920

gccaccttcg aggccgagta cgaccttgag cgcgcccaga aggtggtgaa cgccctcttc 1980

actagcacta accagctagg cctgaagact gacgtgaccg actaccacat cgaccaagtg 2040

agcaacctag tggcctgcct ctccgacgag ttctgcctcg acgagaagcg cgagctgtcc 2100

gagaaggtga agcacgccaa gcgcctctcc gacgagcgca acctgctcca ggaccccaac 2160

ttcaggggca tcaacaggca gcccgaccgc ggctggcgcg gctccaccga catcaccatc 2220

cagggcggtg acgacgtatt caaggagaac tacgttaccc tccccggcac cttcgacgag 2280

tgttacccca cctacctcta ccagaagatc gacgagtcca agctgaaggc ctacacccgc 2340

taccagctcc gcggctacat cgaggactcc caggacctgg aaatctacct catccgctac 2400

aacgccaagc acgagatcgt gaacgtgcct ggcaccggca gcctctggcc tctcagcgtg 2460

gagaaccaga tcggcccttg cggcgagcct aaccgctgcg cccctcacct cgagtggaac 2520

cctgacctcc actgctcgtg cagggacggc gagaagtgcg cccaccatag ccaccacttc 2580

tctctggaca tcgacgtggg ctgcaccgac ctgaacgagg acctgggcgt gtgggttatc 2640

ttcaagatca agacccagga cggtcacgcc aggctgggta acctggagtt ccttgaggaa 2700

aagcctctgc tgggtgaggc cctggccagg gtcaagaggg ctgagaagaa atggagggat 2760

aagagggaga ccctgcagct ggagaccact atcgtctaca aggaggctaa ggagtctgtc 2820

gatgctctgt tcgtcaactc tcagtacgat agactgcaag ctgataccaa catcgctatg 2880

atccacgctg cggataagcg ggtccaccgg atccgggagg cttaccttcc ggagctttct 2940

gtcatcccgg gtgtcaacgc tgcgatcttc gaggaacttg aggaacggat cttcactgcg 3000

tttagtcttt acgatgcgcg gaacatcatc aagaacgggg acttcaacaa tggtctgctg 3060

tgctggaacg tcaagggtca tgtcgaggtc gaggaacaaa acaatcatcg tagtgtcctt 3120

gtcattcctg agtgggaggc ggaggtctct caagaggtcc gtgtttgccc ggggcgtggg 3180

tacattcttc gtgttactgc gtacaaggag gggtacgggg aggggtgcgt tactattcat 3240

gagattgaga acaatactga tgagcttaag ttcaacaatt gtgttgagga ggaggtttac 3300

ccgaacaata ctgttacgtg catcaactac acggcaacgc aagaggaata cgaggggacg 3360

tacacctcgc gtaatagagg gtatgatgag gcgtacggaa acaacccgtc ggttccagca 3420

gattatgcct cggtttatga ggagaagtcg tacacggata gacgacgcga gaatccatgt 3480

gagtcaaatc gaggatacgg agattacaca ccattaccag caggatacgt tacaaaggag 3540

ttggaatact tcccggaaac agataaagtt tggattgaaa tcggagaaac agaaggaaca 3600

ttcatcgtcg actcagtaga attgttgttg atggaagaat ga 3642

<210> 13

<211> 1213

<212> PRT

<213> 人工的

<220>

<223> 嵌合蛋白质变体TIC867_20的氨基酸序列。

<400> 13

Met Thr Ser Asn Arg Lys Asn Glu Asn Glu Ile Ile Asn Ala Leu Ser

1 5 10 15

Ile Pro Ala Val Ser Asn His Ser Ala Gln Met Asn Leu Ser Thr Asp

20 25 30

Ala Arg Ile Glu Asp Ser Leu Cys Ile Ala Glu Gly Asn Asn Ile Asp

35 40 45

Pro Phe Val Ser Ala Ser Thr Val Gln Thr Gly Ile Asn Ile Ala Gly

50 55 60

Arg Ile Leu Gly Val Leu Gly Val Pro Phe Ala Gly Gln Ile Ala Ser

65 70 75 80

Phe Tyr Ser Phe Leu Val Gly Glu Leu Trp Pro Arg Gly Arg Asp Pro

85 90 95

Trp Glu Ile Phe Leu Glu His Val Glu Gln Leu Ile Arg Gln Gln Val

100 105 110

Thr Glu Asn Thr Arg Asp Thr Ala Leu Ala Arg Leu Gln Gly Leu Gly

115 120 125

Asn Ser Phe Arg Ala Tyr Gln Gln Ser Leu Glu Asp Trp Leu Glu Asn

130 135 140

Arg Asp Asp Ala Arg Thr Arg Ser Val Leu Tyr Thr Gln Tyr Ile Ala

145 150 155 160

Leu Glu Leu Asp Phe Leu Asn Ala Met Pro Leu Phe Ala Ile Arg Asn

165 170 175

Gln Glu Val Pro Leu Leu Met Val Tyr Ala Gln Ala Ala Asn Leu His

180 185 190

Leu Leu Leu Leu Arg Asp Ala Ser Leu Phe Gly Ser Glu Phe Gly Leu

195 200 205

Thr Ser Gln Glu Ile Gln Arg Tyr Tyr Glu Arg Gln Val Glu Lys Thr

210 215 220

Arg Glu Tyr Ser Asp Tyr Cys Ala Arg Trp Tyr Asn Thr Gly Leu Asn

225 230 235 240

Asn Leu Arg Gly Thr Asn Ala Glu Ser Trp Leu Arg Tyr Asn Gln Phe

245 250 255

Arg Arg Asp Leu Thr Leu Gly Val Leu Asp Leu Val Ala Leu Phe Pro

260 265 270

Ser Tyr Asp Thr Arg Val Tyr Pro Met Asn Thr Ser Ala Gln Leu Thr

275 280 285

Arg Glu Ile Tyr Thr Asp Pro Ile Gly Arg Thr Asn Ala Pro Ser Gly

290 295 300

Phe Ala Ser Thr Asn Trp Phe Asn Asn Asn Ala Pro Ser Phe Ser Ala

305 310 315 320

Ile Glu Ala Ala Val Ile Arg Pro Pro His Leu Leu Asp Phe Pro Glu

325 330 335

Gln Leu Thr Ile Phe Ser Val Leu Ser Arg Trp Ser Asn Thr Gln Tyr

340 345 350

Met Asn Tyr Trp Val Gly His Arg Leu Glu Ser Arg Thr Ile Arg Gly

355 360 365

Ser Leu Ser Thr Ser Thr His Gly Asn Thr Asn Thr Ser Ile Asn Pro

370 375 380

Val Thr Leu Gln Phe Thr Ser Arg Asp Val Tyr Arg Thr Glu Ser Phe

385 390 395 400

Ala Gly Ile Asn Ile Leu Leu Thr Thr Pro Val Asn Gly Val Pro Trp

405 410 415

Ala Arg Phe Asn Trp Arg Asn Pro Leu Asn Ser Leu Arg Gly Ser Leu

420 425 430

Leu Tyr Thr Ile Gly Tyr Thr Gly Val Gly Thr Gln Leu Phe Asp Ser

435 440 445

Glu Thr Glu Leu Pro Pro Glu Thr Thr Glu Arg Pro Asn Tyr Glu Ser

450 455 460

Tyr Ser His Arg Leu Ser Asn Ile Arg Leu Ile Ser Gly Asn Thr Leu

465 470 475 480

Arg Ala Pro Val Tyr Ser Trp Thr His Arg Ser Ala Asp Arg Thr Asn

485 490 495

Thr Ile Ser Ser Asp Ser Ile Thr Gln Ile Pro Leu Val Lys Ala His

500 505 510

Thr Leu Gln Ser Gly Thr Thr Val Val Lys Gly Pro Gly Phe Thr Gly

515 520 525

Gly Asp Ile Leu Arg Arg Thr Ser Gly Gly Pro Phe Ala Phe Ser Asn

530 535 540

Val Asn Leu Asp Phe Asn Leu Ser Gln Arg Tyr Arg Ala Arg Ile Arg

545 550 555 560

Tyr Ala Ser Thr Thr Asn Leu Arg Ile Tyr Val Thr Val Ala Gly Glu

565 570 575

Arg Ile Phe Ala Gly Gln Phe Asp Lys Thr Met Asp Ala Gly Ala Pro

580 585 590

Leu Thr Phe Gln Ser Phe Ser Tyr Ala Thr Ile Asn Thr Ala Phe Thr

595 600 605

Phe Pro Glu Arg Ser Ser Ser Leu Thr Val Gly Ala Asp Thr Phe Ser

610 615 620

Ser Gly Asn Glu Val Tyr Val Asp Arg Phe Glu Leu Ile Pro Val Thr

625 630 635 640

Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Val Val

645 650 655

Asn Ala Leu Phe Thr Ser Thr Asn Gln Leu Gly Leu Lys Thr Asp Val

660 665 670

Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Ala Cys Leu Ser

675 680 685

Asp Glu Phe Cys Leu Asp Glu Lys Arg Glu Leu Ser Glu Lys Val Lys

690 695 700

His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn

705 710 715 720

Phe Arg Gly Ile Asn Arg Gln Pro Asp Arg Gly Trp Arg Gly Ser Thr

725 730 735

Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val

740 745 750

Thr Leu Pro Gly Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln

755 760 765

Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg

770 775 780

Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr

785 790 795 800

Asn Ala Lys His Glu Ile Val Asn Val Pro Gly Thr Gly Ser Leu Trp

805 810 815

Pro Leu Ser Val Glu Asn Gln Ile Gly Pro Cys Gly Glu Pro Asn Arg

820 825 830

Cys Ala Pro His Leu Glu Trp Asn Pro Asp Leu His Cys Ser Cys Arg

835 840 845

Asp Gly Glu Lys Cys Ala His His Ser His His Phe Ser Leu Asp Ile

850 855 860

Asp Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Val Ile

865 870 875 880

Phe Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn Leu Glu

885 890 895

Phe Leu Glu Glu Lys Pro Leu Leu Gly Glu Ala Leu Ala Arg Val Lys

900 905 910

Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Thr Leu Gln Leu Glu

915 920 925

Thr Thr Ile Val Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala Leu Phe

930 935 940

Val Asn Ser Gln Tyr Asp Arg Leu Gln Ala Asp Thr Asn Ile Ala Met

945 950 955 960

Ile His Ala Ala Asp Lys Arg Val His Arg Ile Arg Glu Ala Tyr Leu

965 970 975

Pro Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile Phe Glu Glu

980 985 990

Leu Glu Glu Arg Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala Arg Asn

995 1000 1005

Ile Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Leu Cys Trp Asn

1010 1015 1020

Val Lys Gly His Val Glu Val Glu Glu Gln Asn Asn His Arg Ser

1025 1030 1035

Val Leu Val Ile Pro Glu Trp Glu Ala Glu Val Ser Gln Glu Val

1040 1045 1050

Arg Val Cys Pro Gly Arg Gly Tyr Ile Leu Arg Val Thr Ala Tyr

1055 1060 1065

Lys Glu Gly Tyr Gly Glu Gly Cys Val Thr Ile His Glu Ile Glu

1070 1075 1080

Asn Asn Thr Asp Glu Leu Lys Phe Asn Asn Cys Val Glu Glu Glu

1085 1090 1095

Val Tyr Pro Asn Asn Thr Val Thr Cys Ile Asn Tyr Thr Ala Thr

1100 1105 1110

Gln Glu Glu Tyr Glu Gly Thr Tyr Thr Ser Arg Asn Arg Gly Tyr

1115 1120 1125

Asp Glu Ala Tyr Gly Asn Asn Pro Ser Val Pro Ala Asp Tyr Ala

1130 1135 1140

Ser Val Tyr Glu Glu Lys Ser Tyr Thr Asp Arg Arg Arg Glu Asn

1145 1150 1155

Pro Cys Glu Ser Asn Arg Gly Tyr Gly Asp Tyr Thr Pro Leu Pro

1160 1165 1170

Ala Gly Tyr Val Thr Lys Glu Leu Glu Tyr Phe Pro Glu Thr Asp

1175 1180 1185

Lys Val Trp Ile Glu Ile Gly Glu Thr Glu Gly Thr Phe Ile Val

1190 1195 1200

Asp Ser Val Glu Leu Leu Leu Met Glu Glu

1205 1210

<210> 14

<211> 3690

<212> DNA

<213> 人工的

<220>

<223> 用于在细菌细胞中表达的编码TIC867_21的重组核苷酸序列。

<400> 14

atgacttcaa ataggaaaaa tgagaatgaa attataaatg ctttatcgat tccagctgta 60

tcgaatcatt ccgcacaaat gaatctatca accgatgctc gtattgagga tagcttgtgt 120

atagccgagg ggaacaatat cgatccattt gttagcgcat caacagtcca aacgggtatt 180

aacatagctg gtagaatact aggtgtatta ggcgtaccgt ttgctggaca aatagctagt 240

ttttatagtt ttcttgttgg tgaattatgg ccccgcggca gagatccttg ggaaattttc 300

ctagaacatg tcgaacaact tataagacaa caagtaacag aaaatactag ggatacggct 360

cttgctcgat tacaaggttt aggaaattcc tttagagcct atcaacagtc acttgaagat 420

tggctagaaa accgtgatga tgcaagaacg agaagtgttc tttataccca atatatagcc 480

ttagaacttg attttcttaa tgcgatgccg cttttcgcaa ttagaaacca agaagttcca 540

ttattaatgg tatatgctca agctgcaaat ttacacctat tattattgag agatgcctct 600

ctttttggta gtgaatttgg gcttacatcc caagaaattc aacgttatta tgagcgccaa 660

gtggaaaaaa cgagagaata ttctgattat tgcgcaagat ggtataatac gggtttaaat 720

aatttgagag ggacaaatgc tgaaagttgg ttgcgatata atcaattccg tagagactta 780

acgctaggag tattagatct agtggcacta ttcccaagct atgacacgcg tgtttatcca 840

atgaatacca gtgctcaatt aacaagagaa atttatacag atccaattgg gagaacaaat 900

gcaccttcag gatttgcaag tacgaattgg tttaataata atgcaccatc gttttctgcc 960

atagaggctg ccgttattag gcctccgcat ctacttgatt ttccagaaca gcttacaatt 1020

ttcagcgtat taagtcgatg gagtaatact caatatatga attactgggt gggacataga 1080

cttgaatcgc gaacaataag ggggtcatta agtacctcga cacacggaaa taccaatact 1140

tctattaatc ctgtaacatt acagttcaca tctcgagacg tttatagaac agaatcattt 1200

gcagggataa atatacttct aactactcct gtgaatggag taccttgggc tagatttaat 1260

tggagaaatc ccctgaattc tcttagaggt agccttctct atactatagg gtatactgga 1320

gtggggacac aactatttga ttcagaaact gaattaccac cagaaacaac agaacgacca 1380

aattatgaat cttacagtca tagattatct aatataagac taatatcagg aaacactttg 1440

agagcaccag tatattcttg gacgcaccgt agtgcagatc gtacaaatac cattagttca 1500

gatagcatta cacaaatacc attggtaaag gcgcataccc tccaatcggg taccactgta 1560

gtaaaagggc cagggtttac aggaggggat atcctccgtc gaacaagtgg aggaccattt 1620

gcttttagta atgttaatct agattttaac ttgtcacaaa ggtatcgtgc tagaattcgt 1680

tatgcctcta ctactaacct aagaatttac gtaacggttg caggtgaacg aatttttgct 1740

ggtcaatttg acaaaactat ggatgctggt gccccattaa cattccaatc ttttagttac 1800

gcaactatta atacagcttt tacattccca gaaagatcga gcagcttgac tgtaggtgcc 1860

gatacgttta gttcaggtaa tgaagtttat gtagatagat ttgaattaat cccagttact 1920

gcaaccggaa cgacaaccta tgagtatgaa gagaagcaga atctagaaaa agcgcagaaa 1980

gcgttgaacg ctttgtttac ggatggcacg aatggctatc tacaaatgga tgccactgat 2040

tatgatatca atcaaactgc aaacttaata gaatgtgtat cagatgaatt gtatgcaaaa 2100

gaaaagatag ttttattaga tgaagtcaaa tatgcgaagc ggcttagcat atcacgtaac 2160

ctacttttga acgatgattt agaattttca gatggatttg gagaaaacgg atggacgaca 2220

agtgataata tttcaatcca ggcggataat ccccttttta aggggaatta tttaaaaatg 2280

tttggggcaa gagatattga tggaacccta tttccaactt atctctatca aaaaatagat 2340

gagtccaggt taaaaccata tacacgttat cgagtaagag ggtttgtggg aagtagtaaa 2400

aatctaaaat tagtggtaac acgctatgag aaagaaattg atgccattat gaatgttcca 2460

aatgatttgg cacatatgca gcttaaccct tcatgtggag attatcgctg tgaatcatcg 2520

tcccagtttt tggtgaacca agtgcatcct acaccaacag ctggatatgc tcttgatatg 2580

tatgcatgcc cgtcaagttc agataaaaaa catattatgt gtcacgatcg tcatccattt 2640

gattttcata ttgacaccgg agaattaaat ccaaacacaa acctgggtat tgatgtcttg 2700

tttaaaattt ctaatccaaa tggatacgct acattaggga atctagaagt cattgaagaa 2760

ggaccactaa cagatgaagc attggtacat gtaaaacaaa aggaaaagaa atggcgtcag 2820

cacatggaga aaaaacgaat ggaaacacaa caagcctatg atccagcaaa acaagctgta 2880

gatgcattat ttacaaatga acaagagtta gactatcata ctactttaga tcatattcag 2940

aacgccgatc agctggtaca ggcgattccc tatgtacacc atgcttggtt accggatgct 3000

ccaggtatga actatgatgt atatcaaggg ttaaacgcac gtatcatgca ggcgtacaat 3060

ttatatgatg cacgaaatgt cataataaat ggtgacttta cacaaggact acaaggatgg 3120

cacgcaacag gaaaagcagc ggtacaacaa atagatggag cttcagtatt agttctatca 3180

aactggagtg ccgaggtatc tcagaatctg catgcccaag atcatcatgg atatatgtta 3240

cgtgtgattg ccaaaaaaga aggtcctgga aaagggtatg taatgatgat ggattttaat 3300

ggaaagcagg aaacacttac gttcacttct tgtgaagaag gatatataac aaaaacaata 3360

gaggtattcc cggaaagtga tcgaatacga attgaaatgg gagaaacaga gggtacgttt 3420

tatgtagata gcatcgagtt gctttgtatg caaggatatg ctagcgataa taacccgcac 3480

acgggtaata tgtatgagca aagttataat ggaaattata atcaaaatac tagcgatgtg 3540

tatcaccaag gatatataaa caactataac caaaattcta gtagtatgta taatcaaaat 3600

tatattaaca atgatgacct gcattccggt tgcacatgta accaagggca taactctggc 3660

tgtacatgta atcaaggata taaccgttag 3690

<210> 15

<211> 3690

<212> DNA

<213> 人工的

<220>

<223> 设计用于在植物细胞中表达的编码TIC867_21的合成核苷酸序列。

<400> 15

atgaccagca accgaaagaa cgagaacgag atcatcaacg ccctgtccat accggccgtg 60

tcaaaccact ccgcccagat gaacctctcc accgacgcga ggatcgagga ctccctctgc 120

atcgccgagg gcaacaacat cgacccgttc gtgtctgcaa gcacggtcca gaccggcatc 180

aacatcgcgg gccgcatcct gggcgtgctc ggcgtgccct tcgcgggtca aatcgcctct 240

ttctactcat tcctcgtggg cgagctgtgg ccgcgcggac gtgacccgtg ggaaatcttc 300

ctggagcacg ttgagcagct catccggcag caagtgaccg agaacaccag ggacaccgca 360

ctggcacggc tccagggcct tggcaacagc ttccgcgcct accagcagtc gctggaggac 420

tggctggaga accgagacga cgccagaacc cgctcagttc tgtacacaca gtacatcgcc 480

ctagagctgg acttcctcaa cgctatgccg ctcttcgcca tccgtaacca ggaagtaccg 540

cttctgatgg tgtacgcaca agcagcgaac ctccatctgc tcctgctgcg agacgcatct 600

ctgttcggca gtgagttcgg gctgacgagc caggagatcc agcgctacta cgagcgccaa 660

gtggagaaga ctcgtgagta cagcgactac tgcgcgcgct ggtacaacac gggcttgaac 720

aaccttcgcg ggacaaacgc cgaatcctgg cttcgctaca accagttccg ccgcgacctc 780

acgctgggtg tgctggacct ggtcgcgctc ttcccgtcct acgacacacg ggtgtaccca 840

atgaacacga gcgcacagct cacccgtgag atctacacag atcccatcgg ccgcaccaac 900

gctcccagtg gcttcgcaag cacgaattgg ttcaacaata acgctccttc tttctctgcc 960

atcgaggccg ctgtcatcag accgccgcac ttactcgatt tcccggagca gctcactatc 1020

ttctctgtgt tgtcccggtg gtcgaacacg cagtacatga actactgggt gggccacagg 1080

ctagagagcc ggaccatccg tggcagtctc tcaacctcga cccacggcaa cacgaacacg 1140

agcatcaacc ctgtcactct ccagtttaca tctagggacg tttacaggac agagtcgttc 1200

gctggcatta acattctgtt gaccactccg gtgaacggcg tcccttgggc ccgcttcaac 1260

tggaggaatc ctctgaactc actgcgcggc agccttctct acactatcgg ctacaccggc 1320

gttgggacgc aactcttcga ctcggagacc gagctgccgc ccgagaccac cgagcggcct 1380

aactacgaga gttattcaca caggctctcc aacatccgct tgatttctgg gaacaccttg 1440

cgggctccgg tgtactcctg gacgcaccgc agcgccgaca gaactaatac catcagctcc 1500

gactcgatca cccagatccc gctggtgaag gctcacacgc ttcagtcggg caccacagtc 1560

gtcaagggcc ctggcttcac cggcggcgac atcctgcgtc gcacatctgg cggacccttc 1620

gccttcagca acgtgaactt ggacttcaat ttgtcacagc ggtatcgtgc cagaatccgg 1680

tacgccagca ctacgaacct gcgaatctat gttactgtgg cgggcgagcg gatcttcgcc 1740

gggcaattcg acaagacgat ggacgcggga gcacctctga cattccagtc attctcttac 1800

gccacgatca acacggcatt cacgtttccg gagcgttcca gtagcctgac cgtgggcgct 1860

gataccttca gtagcgggaa cgaggtgtac gttgaccgtt tcgagctgat cccggtcacc 1920

gccaccggga ctaccaccta cgagtacgag gagaagcaga atctcgagaa ggctcagaag 1980

gctctgaacg ctctgttcac tgacgggacc aacggctacc tccagatgga cgccactgac 2040

tacgacatca accagacagc taacctgatt gagtgtgtga gtgacgaact gtacgctaag 2100

gagaagatcg tactcctgga cgaggtgaag tacgctaagc gcctgagcat tagccgtaac 2160

ctgctgctga acgacgatct ggagttcagc gacggctttg gcgagaacgg ctggaccacc 2220

agcgacaaca tctccatcca ggccgacaat ccactcttca aaggcaacta cctcaagatg 2280

ttcggagcca gggacatcga cggcaccctc tttccgacct acctctacca gaagatcgac 2340

gagtcccgcc tcaaacccta cacccgctac agggtgcgcg gcttcgtggg cagcagcaag 2400

aacctcaagc tcgtggtcac acggtatgag aaggagatcg acgccatcat gaacgtgccc 2460

aacgatctcg cccacatgca gctcaatcca tcctgcggcg actaccggtg cgagtccagc 2520

tcccagttcc tcgtgaacca ggtgcaccct actccgaccg ctggctatgc cctggacatg 2580

tacgcctgcc ctagttcctc cgacaagaag cacatcatgt gccacgaccg tcatccgttc 2640

gacttccaca tcgacaccgg cgaactgaac ccgaacacca acctgggcat cgacgtactg 2700

ttcaagattt ccaacccgaa cgggtacgcc accttgggca acctggaggt catcgaagaa 2760

ggcccgctga ccgacgaggc cctggtccac gtcaaacaga aggagaagaa gtggcggcag 2820

cacatggaga agaagcggat ggagactcaa caagcctacg acccggccaa gcaagctgtg 2880

gacgctctgt tcaccaacga gcaagagctt gactaccaca ctactcttga ccacatccag 2940

aatgctgacc agcttgtcca ggctattccg tacgtccacc acgcttggct accggacgct 3000

ccagggatga actacgatgt gtaccagggt ctgaacgcgc ggatcatgca agcgtacaac 3060

ctgtacgacg cgcgtaacgt catcatcaac ggtgacttca ctcagggtct tcaaggttgg 3120

cacgcgactg gcaaagcggc agtccagcag attgatggtg cgtctgttct tgtgttgagc 3180

aactggtctg cggaggtttc tcagaacctg cacgcacagg atcaccacgg ctacatgctg 3240

agggtgattg ctaagaagga gggccctggc aaaggctacg tcatgatgat ggacttcaac 3300

ggaaagcaag aaaccctgac cttcactagc tgtgaggagg gctacatcac taagaccatt 3360

gaggtctttc cggagtctga ccgcatccgg atcgagatgg gcgagaccga aggcacgttc 3420

tacgtggact ccatcgaact cctctgcatg caaggctacg cctccgacaa caacccacac 3480

acgggcaaca tgtacgagca gtcctacaac gggaactaca accagaacac ctccgatgtg 3540

taccatcagg gctacatcaa caactacaac cagaacagca gcagcatgta caaccagaac 3600

tacatcaaca acgatgactt gcactcgggt tgcacctgca accagggtca caacagtggg 3660

tgcacgtgca accagggata caaccgttga 3690

<210> 16

<211> 1229

<212> PRT

<213> 人工的

<220>

<223> 嵌合蛋白质变体TIC867_21的氨基酸序列。

<400> 16

Met Thr Ser Asn Arg Lys Asn Glu Asn Glu Ile Ile Asn Ala Leu Ser

1 5 10 15

Ile Pro Ala Val Ser Asn His Ser Ala Gln Met Asn Leu Ser Thr Asp

20 25 30

Ala Arg Ile Glu Asp Ser Leu Cys Ile Ala Glu Gly Asn Asn Ile Asp

35 40 45

Pro Phe Val Ser Ala Ser Thr Val Gln Thr Gly Ile Asn Ile Ala Gly

50 55 60

Arg Ile Leu Gly Val Leu Gly Val Pro Phe Ala Gly Gln Ile Ala Ser

65 70 75 80

Phe Tyr Ser Phe Leu Val Gly Glu Leu Trp Pro Arg Gly Arg Asp Pro

85 90 95

Trp Glu Ile Phe Leu Glu His Val Glu Gln Leu Ile Arg Gln Gln Val

100 105 110

Thr Glu Asn Thr Arg Asp Thr Ala Leu Ala Arg Leu Gln Gly Leu Gly

115 120 125

Asn Ser Phe Arg Ala Tyr Gln Gln Ser Leu Glu Asp Trp Leu Glu Asn

130 135 140

Arg Asp Asp Ala Arg Thr Arg Ser Val Leu Tyr Thr Gln Tyr Ile Ala

145 150 155 160

Leu Glu Leu Asp Phe Leu Asn Ala Met Pro Leu Phe Ala Ile Arg Asn

165 170 175

Gln Glu Val Pro Leu Leu Met Val Tyr Ala Gln Ala Ala Asn Leu His

180 185 190

Leu Leu Leu Leu Arg Asp Ala Ser Leu Phe Gly Ser Glu Phe Gly Leu

195 200 205

Thr Ser Gln Glu Ile Gln Arg Tyr Tyr Glu Arg Gln Val Glu Lys Thr

210 215 220

Arg Glu Tyr Ser Asp Tyr Cys Ala Arg Trp Tyr Asn Thr Gly Leu Asn

225 230 235 240

Asn Leu Arg Gly Thr Asn Ala Glu Ser Trp Leu Arg Tyr Asn Gln Phe

245 250 255

Arg Arg Asp Leu Thr Leu Gly Val Leu Asp Leu Val Ala Leu Phe Pro

260 265 270

Ser Tyr Asp Thr Arg Val Tyr Pro Met Asn Thr Ser Ala Gln Leu Thr

275 280 285

Arg Glu Ile Tyr Thr Asp Pro Ile Gly Arg Thr Asn Ala Pro Ser Gly

290 295 300

Phe Ala Ser Thr Asn Trp Phe Asn Asn Asn Ala Pro Ser Phe Ser Ala

305 310 315 320

Ile Glu Ala Ala Val Ile Arg Pro Pro His Leu Leu Asp Phe Pro Glu

325 330 335

Gln Leu Thr Ile Phe Ser Val Leu Ser Arg Trp Ser Asn Thr Gln Tyr

340 345 350

Met Asn Tyr Trp Val Gly His Arg Leu Glu Ser Arg Thr Ile Arg Gly

355 360 365

Ser Leu Ser Thr Ser Thr His Gly Asn Thr Asn Thr Ser Ile Asn Pro

370 375 380

Val Thr Leu Gln Phe Thr Ser Arg Asp Val Tyr Arg Thr Glu Ser Phe

385 390 395 400

Ala Gly Ile Asn Ile Leu Leu Thr Thr Pro Val Asn Gly Val Pro Trp

405 410 415

Ala Arg Phe Asn Trp Arg Asn Pro Leu Asn Ser Leu Arg Gly Ser Leu

420 425 430

Leu Tyr Thr Ile Gly Tyr Thr Gly Val Gly Thr Gln Leu Phe Asp Ser

435 440 445

Glu Thr Glu Leu Pro Pro Glu Thr Thr Glu Arg Pro Asn Tyr Glu Ser

450 455 460

Tyr Ser His Arg Leu Ser Asn Ile Arg Leu Ile Ser Gly Asn Thr Leu

465 470 475 480

Arg Ala Pro Val Tyr Ser Trp Thr His Arg Ser Ala Asp Arg Thr Asn

485 490 495

Thr Ile Ser Ser Asp Ser Ile Thr Gln Ile Pro Leu Val Lys Ala His

500 505 510

Thr Leu Gln Ser Gly Thr Thr Val Val Lys Gly Pro Gly Phe Thr Gly

515 520 525

Gly Asp Ile Leu Arg Arg Thr Ser Gly Gly Pro Phe Ala Phe Ser Asn

530 535 540

Val Asn Leu Asp Phe Asn Leu Ser Gln Arg Tyr Arg Ala Arg Ile Arg

545 550 555 560

Tyr Ala Ser Thr Thr Asn Leu Arg Ile Tyr Val Thr Val Ala Gly Glu

565 570 575

Arg Ile Phe Ala Gly Gln Phe Asp Lys Thr Met Asp Ala Gly Ala Pro

580 585 590

Leu Thr Phe Gln Ser Phe Ser Tyr Ala Thr Ile Asn Thr Ala Phe Thr

595 600 605

Phe Pro Glu Arg Ser Ser Ser Leu Thr Val Gly Ala Asp Thr Phe Ser

610 615 620

Ser Gly Asn Glu Val Tyr Val Asp Arg Phe Glu Leu Ile Pro Val Thr

625 630 635 640

Ala Thr Gly Thr Thr Thr Tyr Glu Tyr Glu Glu Lys Gln Asn Leu Glu

645 650 655

Lys Ala Gln Lys Ala Leu Asn Ala Leu Phe Thr Asp Gly Thr Asn Gly

660 665 670

Tyr Leu Gln Met Asp Ala Thr Asp Tyr Asp Ile Asn Gln Thr Ala Asn

675 680 685

Leu Ile Glu Cys Val Ser Asp Glu Leu Tyr Ala Lys Glu Lys Ile Val

690 695 700

Leu Leu Asp Glu Val Lys Tyr Ala Lys Arg Leu Ser Ile Ser Arg Asn

705 710 715 720

Leu Leu Leu Asn Asp Asp Leu Glu Phe Ser Asp Gly Phe Gly Glu Asn

725 730 735

Gly Trp Thr Thr Ser Asp Asn Ile Ser Ile Gln Ala Asp Asn Pro Leu

740 745 750

Phe Lys Gly Asn Tyr Leu Lys Met Phe Gly Ala Arg Asp Ile Asp Gly

755 760 765

Thr Leu Phe Pro Thr Tyr Leu Tyr Gln Lys Ile Asp Glu Ser Arg Leu

770 775 780

Lys Pro Tyr Thr Arg Tyr Arg Val Arg Gly Phe Val Gly Ser Ser Lys

785 790 795 800

Asn Leu Lys Leu Val Val Thr Arg Tyr Glu Lys Glu Ile Asp Ala Ile

805 810 815

Met Asn Val Pro Asn Asp Leu Ala His Met Gln Leu Asn Pro Ser Cys

820 825 830

Gly Asp Tyr Arg Cys Glu Ser Ser Ser Gln Phe Leu Val Asn Gln Val

835 840 845

His Pro Thr Pro Thr Ala Gly Tyr Ala Leu Asp Met Tyr Ala Cys Pro

850 855 860

Ser Ser Ser Asp Lys Lys His Ile Met Cys His Asp Arg His Pro Phe

865 870 875 880

Asp Phe His Ile Asp Thr Gly Glu Leu Asn Pro Asn Thr Asn Leu Gly

885 890 895

Ile Asp Val Leu Phe Lys Ile Ser Asn Pro Asn Gly Tyr Ala Thr Leu

900 905 910

Gly Asn Leu Glu Val Ile Glu Glu Gly Pro Leu Thr Asp Glu Ala Leu

915 920 925

Val His Val Lys Gln Lys Glu Lys Lys Trp Arg Gln His Met Glu Lys

930 935 940

Lys Arg Met Glu Thr Gln Gln Ala Tyr Asp Pro Ala Lys Gln Ala Val

945 950 955 960

Asp Ala Leu Phe Thr Asn Glu Gln Glu Leu Asp Tyr His Thr Thr Leu

965 970 975

Asp His Ile Gln Asn Ala Asp Gln Leu Val Gln Ala Ile Pro Tyr Val

980 985 990

His His Ala Trp Leu Pro Asp Ala Pro Gly Met Asn Tyr Asp Val Tyr

995 1000 1005

Gln Gly Leu Asn Ala Arg Ile Met Gln Ala Tyr Asn Leu Tyr Asp

1010 1015 1020

Ala Arg Asn Val Ile Ile Asn Gly Asp Phe Thr Gln Gly Leu Gln

1025 1030 1035

Gly Trp His Ala Thr Gly Lys Ala Ala Val Gln Gln Ile Asp Gly

1040 1045 1050

Ala Ser Val Leu Val Leu Ser Asn Trp Ser Ala Glu Val Ser Gln

1055 1060 1065

Asn Leu His Ala Gln Asp His His Gly Tyr Met Leu Arg Val Ile

1070 1075 1080

Ala Lys Lys Glu Gly Pro Gly Lys Gly Tyr Val Met Met Met Asp

1085 1090 1095

Phe Asn Gly Lys Gln Glu Thr Leu Thr Phe Thr Ser Cys Glu Glu

1100 1105 1110

Gly Tyr Ile Thr Lys Thr Ile Glu Val Phe Pro Glu Ser Asp Arg

1115 1120 1125

Ile Arg Ile Glu Met Gly Glu Thr Glu Gly Thr Phe Tyr Val Asp

1130 1135 1140

Ser Ile Glu Leu Leu Cys Met Gln Gly Tyr Ala Ser Asp Asn Asn

1145 1150 1155

Pro His Thr Gly Asn Met Tyr Glu Gln Ser Tyr Asn Gly Asn Tyr

1160 1165 1170

Asn Gln Asn Thr Ser Asp Val Tyr His Gln Gly Tyr Ile Asn Asn

1175 1180 1185

Tyr Asn Gln Asn Ser Ser Ser Met Tyr Asn Gln Asn Tyr Ile Asn

1190 1195 1200

Asn Asp Asp Leu His Ser Gly Cys Thr Cys Asn Gln Gly His Asn

1205 1210 1215

Ser Gly Cys Thr Cys Asn Gln Gly Tyr Asn Arg

1220 1225

<210> 17

<211> 3432

<212> DNA

<213> 人工的

<220>

<223> 用于在细菌细胞中表达的编码TIC867_22的重组核苷酸序列。

<400> 17

atgacttcaa ataggaaaaa tgagaatgaa attataaatg ctttatcgat tccagctgta 60

tcgaatcatt ccgcacaaat gaatctatca accgatgctc gtattgagga tagcttgtgt 120

atagccgagg ggaacaatat cgatccattt gttagcgcat caacagtcca aacgggtatt 180

aacatagctg gtagaatact aggtgtatta ggcgtaccgt ttgctggaca aatagctagt 240

ttttatagtt ttcttgttgg tgaattatgg ccccgcggca gagatccttg ggaaattttc 300

ctagaacatg tcgaacaact tataagacaa caagtaacag aaaatactag ggatacggct 360

cttgctcgat tacaaggttt aggaaattcc tttagagcct atcaacagtc acttgaagat 420

tggctagaaa accgtgatga tgcaagaacg agaagtgttc tttataccca atatatagcc 480

ttagaacttg attttcttaa tgcgatgccg cttttcgcaa ttagaaacca agaagttcca 540

ttattaatgg tatatgctca agctgcaaat ttacacctat tattattgag agatgcctct 600

ctttttggta gtgaatttgg gcttacatcc caagaaattc aacgttatta tgagcgccaa 660

gtggaaaaaa cgagagaata ttctgattat tgcgcaagat ggtataatac gggtttaaat 720

aatttgagag ggacaaatgc tgaaagttgg ttgcgatata atcaattccg tagagactta 780

acgctaggag tattagatct agtggcacta ttcccaagct atgacacgcg tgtttatcca 840

atgaatacca gtgctcaatt aacaagagaa atttatacag atccaattgg gagaacaaat 900

gcaccttcag gatttgcaag tacgaattgg tttaataata atgcaccatc gttttctgcc 960

atagaggctg ccgttattag gcctccgcat ctacttgatt ttccagaaca gcttacaatt 1020

ttcagcgtat taagtcgatg gagtaatact caatatatga attactgggt gggacataga 1080

cttgaatcgc gaacaataag ggggtcatta agtacctcga cacacggaaa taccaatact 1140

tctattaatc ctgtaacatt acagttcaca tctcgagacg tttatagaac agaatcattt 1200

gcagggataa atatacttct aactactcct gtgaatggag taccttgggc tagatttaat 1260

tggagaaatc ccctgaattc tcttagaggt agccttctct atactatagg gtatactgga 1320

gtggggacac aactatttga ttcagaaact gaattaccac cagaaacaac agaacgacca 1380

aattatgaat cttacagtca tagattatct aatataagac taatatcagg aaacactttg 1440

agagcaccag tatattcttg gacgcaccgt agtgcagatc gtacaaatac cattagttca 1500

gatagcatta cacaaatacc attggtaaag gcgcataccc tccaatcggg taccactgta 1560

gtaaaagggc cagggtttac aggaggggat atcctccgtc gaacaagtgg aggaccattt 1620

gcttttagta atgttaatct agattttaac ttgtcacaaa ggtatcgtgc tagaattcgt 1680

tatgcctcta ctactaacct aagaatttac gtaacggttg caggtgaacg aatttttgct 1740

ggtcaatttg acaaaactat ggatgctggt gccccattaa cattccaatc ttttagttac 1800

gcaactatta atacagcttt tacattccca gaaagatcga gcagcttgac tgtaggtgcc 1860

gatacgttta gttcaggtaa tgaagtttat gtagatagat ttgaattaat cccagttact 1920

gcaaccaatc cgacgcgaga ggcggaagag gatctagaag cagcgaagaa agcggtggcg 1980

agcttgttta cacgtacaag ggacggatta caagtaaatg tgacagatta tcaagtcgat 2040

caagcggcaa atttagtgtc atgcttatca gatgaacaat atgggcatga caaaaagatg 2100

ttattggaag cggtaagagc ggcaaaacgc ctcagccgag aacgcaactt acttcaggat 2160

ccagatttta atacaatcaa tagtacagaa gaaaatggat ggaaagcaag taacggcgtt 2220

actattagcg agggcggtcc attctataaa ggccgtgcgc ttcagctagc aagcgcaaga 2280

gaaaattacc caacatacat ttatcaaaaa gtaaatgcat cagagttaaa gccgtataca 2340

cgttatagac tggatgggtt cgtgaagagt agtcaagatt tagaaattga tctcattcac 2400

catcataaag tccatctcgt gaaaaatgta ccagataatt tagtatccga tacttactcg 2460

gatggttctt gcagtggaat gaatcgatgt gaggaacaac agatggtaaa tgcgcaactg 2520

gaaacagaac atcatcatcc gatggattgc tgtgaagcgg ctcaaacaca tgagttttct 2580

tcctatatta atacaggcga tctaaattca agtgtagatc aaggcatttg ggttgtattg 2640

aaagttcgaa caaccgatgg ttatgcgacg ctaggaaatc ttgaattggt agaggtcgga 2700

ccgttatcgg gtgaatctct agaacgtgaa caaagggata atgcgaaatg gagtgcagag 2760

ctaggaagaa agcgtgcaga aacagatcgc gtgtatcaag atgccaaaca atccatcaat 2820

catttatttg tggattatca agatcaacaa ttaaatccag aaatagggat ggcagatatt 2880

attgacgctc aaaatcttgt cgcatcaatt tcagatgtgt atagcgatgc agtactgcaa 2940

atccctggaa ttaactatga gatttacaca gagctatcca atcgcttaca acaagcatcg 3000

tatctgtata cgtctcgaaa tgcggtgcaa aatggggact ttaacagcgg tctagatagt 3060

tggaatgcaa cagggggggc tacggtacaa caggatggca atacgcattt cttagttctt 3120

tctcattggg atgcacaagt ttctcaacaa tttagagtgc agccgaattg taaatatgta 3180

ttacgtgtaa cagcagagaa agtaggcggc ggagacggat acgtgacaat ccgggatggt 3240

gctcatcata cagaaaagct tacatttaat gcatgtgatt atgatataaa tggcacgtac 3300

gtgactgata atacgtatct aacaaaagaa gtggtattct attcacatac agaacacatg 3360

tgggtagagg taagtgaaac agaaggtgca tttcatatag atagtattga attcgttgaa 3420

acagaaaagt ag 3432

<210> 18

<211> 3432

<212> DNA

<213> 人工的

<220>

<223> 设计用于在植物细胞中表达的编码TIC867_22的合成核苷酸序列。

<400> 18

atgaccagca accgaaagaa cgagaacgag atcatcaacg ccctgtccat accggccgtg 60

tcaaaccact ccgcccagat gaacctctcc accgacgcga ggatcgagga ctccctctgc 120

atcgccgagg gcaacaacat cgacccgttc gtgtctgcaa gcacggtcca gaccggcatc 180

aacatcgcgg gccgcatcct gggcgtgctc ggcgtgccct tcgcgggtca aatcgcctct 240

ttctactcat tcctcgtggg cgagctgtgg ccgcgcggac gtgacccgtg ggaaatcttc 300

ctggagcacg ttgagcagct catccggcag caagtgaccg agaacaccag ggacaccgca 360

ctggcacggc tccagggcct tggcaacagc ttccgcgcct accagcagtc gctggaggac 420

tggctggaga accgagacga cgccagaacc cgctcagttc tgtacacaca gtacatcgcc 480

ctagagctgg acttcctcaa cgctatgccg ctcttcgcca tccgtaacca ggaagtaccg 540

cttctgatgg tgtacgcaca agcagcgaac ctccatctgc tcctgctgcg agacgcatct 600

ctgttcggca gtgagttcgg gctgacgagc caggagatcc agcgctacta cgagcgccaa 660

gtggagaaga ctcgtgagta cagcgactac tgcgcgcgct ggtacaacac gggcttgaac 720

aaccttcgcg ggacaaacgc cgaatcctgg cttcgctaca accagttccg ccgcgacctc 780

acgctgggtg tgctggacct ggtcgcgctc ttcccgtcct acgacacacg ggtgtaccca 840

atgaacacga gcgcacagct cacccgtgag atctacacag atcccatcgg ccgcaccaac 900

gctcccagtg gcttcgcaag cacgaattgg ttcaacaata acgctccttc tttctctgcc 960

atcgaggccg ctgtcatcag accgccgcac ttactcgatt tcccggagca gctcactatc 1020

ttctctgtgt tgtcccggtg gtcgaacacg cagtacatga actactgggt gggccacagg 1080

ctagagagcc ggaccatccg tggcagtctc tcaacctcga cccacggcaa cacgaacacg 1140

agcatcaacc ctgtcactct ccagtttaca tctagggacg tttacaggac agagtcgttc 1200

gctggcatta acattctgtt gaccactccg gtgaacggcg tcccttgggc ccgcttcaac 1260

tggaggaatc ctctgaactc actgcgcggc agccttctct acactatcgg ctacaccggc 1320

gttgggacgc aactcttcga ctcggagacc gagctgccgc ccgagaccac cgagcggcct 1380

aactacgaga gttattcaca caggctctcc aacatccgct tgatttctgg gaacaccttg 1440

cgggctccgg tgtactcctg gacgcaccgc agcgccgaca gaactaatac catcagctcc 1500

gactcgatca cccagatccc gctggtgaag gctcacacgc ttcagtcggg caccacagtc 1560

gtcaagggcc ctggcttcac cggcggcgac atcctgcgtc gcacatctgg cggacccttc 1620

gccttcagca acgtgaactt ggacttcaat ttgtcacagc ggtatcgtgc cagaatccgg 1680

tacgccagca ctacgaacct gcgaatctat gttactgtgg cgggcgagcg gatcttcgcc 1740

gggcaattcg acaagacgat ggacgcggga gcacctctga cattccagtc attctcttac 1800

gccacgatca acacggcatt cacgtttccg gagcgttcca gtagcctgac cgtgggcgct 1860

gataccttca gtagcgggaa cgaggtgtac gttgaccgtt tcgagctgat cccggtcacc 1920

gccaccaacc cgacgcggga agctgaggaa gacttggaag ccgccaagaa agcggtcgcc 1980

agcctgttta ctcggacgcg ggacgggctc caagtgaatg tgacggacta tcaagtggat 2040

caggccgcta acctcgtgtc atgcctgagc gacgagcagt acggtcacga caagaaaatg 2100

ctgctggagg ccgtccgggc cgccaagcgg ctgtccaggg agcgtaacct gctacaagat 2160

cccgacttta acacgatcaa cagcacagag gagaatggct ggaaggccag caacggagtt 2220

acgataagcg agggcggtcc gttctacaag ggtcgtgccc tccagctcgc ctctgcaagg 2280

gagaactatc caacctacat ctatcagaag gtgaacgcat ccgagcttaa gccctacaca 2340

cgctaccgcc tggacgggtt cgttaagtcc agtcaagacc tagagataga cctcatccac 2400

caccacaaag tgcatctggt caagaacgtt cccgataatc tcgtgagcga tacctactca 2460

gacggctcat gctctggcat gaacagatgt gaggagcaac agatggttaa tgctcaactc 2520

gaaaccgagc atcatcatcc tatggattgc tgcgaggccg cgcagaccca tgagttcagc 2580

tcttacatca acaccggaga cctcaacagt agcgtggatc agggaatttg ggtggtgctt 2640

aaagtgcgta caaccgacgg ctacgccacc ctcggcaacc ttgagcttgt cgaggtcgga 2700

ccacttagcg gcgagtccct ggaacgtgag cagcgggaca acgccaaatg gagcgcagag 2760

ctagggcgca aacgcgcgga gacggaccgg gtttatcagg acgcgaagca gtccatcaat 2820

cacctcttcg tggattatca ggaccagcag cttaatccag agatcggcat ggccgacatc 2880

atcgacgccc agaacctagt agcgtcgatt tccgatgtct attccgacgc cgtgcttcaa 2940

atacctggca tcaactacga gatctacaca gagttgtcca acaggctcca gcaagcgtca 3000

tacctctaca ccagccgcaa cgccgtccag aatggcgact tcaattccgg actagactcc 3060

tggaacgcca cgggcggagc tacggtgcaa caagacggca acacccactt cctcgtactt 3120

agccactggg acgctcaagt gagtcagcaa ttccgggttc agccgaactg caagtacgtc 3180

ctgcgcgtaa cggccgagaa ggttggaggc ggagacggct acgttaccat ccgcgacggc 3240

gctcaccaca ccgagaaact gacgttcaac gcttgtgact acgacatcaa cggcacttac 3300

gtgacggaca acacctacct gacgaaggag gtggtgttct attctcacac cgagcacatg 3360

tgggttgagg tcagcgagac cgagggagcc ttccacattg acagcatcga gttcgtggag 3420

actgagaagt ga 3432

<210> 19

<211> 1143

<212> PRT

<213> 人工的

<220>

<223> 嵌合蛋白质变体TIC867_22的氨基酸序列。

<400> 19

Met Thr Ser Asn Arg Lys Asn Glu Asn Glu Ile Ile Asn Ala Leu Ser

1 5 10 15

Ile Pro Ala Val Ser Asn His Ser Ala Gln Met Asn Leu Ser Thr Asp

20 25 30

Ala Arg Ile Glu Asp Ser Leu Cys Ile Ala Glu Gly Asn Asn Ile Asp

35 40 45

Pro Phe Val Ser Ala Ser Thr Val Gln Thr Gly Ile Asn Ile Ala Gly

50 55 60

Arg Ile Leu Gly Val Leu Gly Val Pro Phe Ala Gly Gln Ile Ala Ser

65 70 75 80

Phe Tyr Ser Phe Leu Val Gly Glu Leu Trp Pro Arg Gly Arg Asp Pro

85 90 95

Trp Glu Ile Phe Leu Glu His Val Glu Gln Leu Ile Arg Gln Gln Val

100 105 110

Thr Glu Asn Thr Arg Asp Thr Ala Leu Ala Arg Leu Gln Gly Leu Gly

115 120 125

Asn Ser Phe Arg Ala Tyr Gln Gln Ser Leu Glu Asp Trp Leu Glu Asn

130 135 140

Arg Asp Asp Ala Arg Thr Arg Ser Val Leu Tyr Thr Gln Tyr Ile Ala

145 150 155 160

Leu Glu Leu Asp Phe Leu Asn Ala Met Pro Leu Phe Ala Ile Arg Asn

165 170 175

Gln Glu Val Pro Leu Leu Met Val Tyr Ala Gln Ala Ala Asn Leu His

180 185 190

Leu Leu Leu Leu Arg Asp Ala Ser Leu Phe Gly Ser Glu Phe Gly Leu

195 200 205

Thr Ser Gln Glu Ile Gln Arg Tyr Tyr Glu Arg Gln Val Glu Lys Thr

210 215 220

Arg Glu Tyr Ser Asp Tyr Cys Ala Arg Trp Tyr Asn Thr Gly Leu Asn

225 230 235 240

Asn Leu Arg Gly Thr Asn Ala Glu Ser Trp Leu Arg Tyr Asn Gln Phe

245 250 255

Arg Arg Asp Leu Thr Leu Gly Val Leu Asp Leu Val Ala Leu Phe Pro

260 265 270

Ser Tyr Asp Thr Arg Val Tyr Pro Met Asn Thr Ser Ala Gln Leu Thr

275 280 285

Arg Glu Ile Tyr Thr Asp Pro Ile Gly Arg Thr Asn Ala Pro Ser Gly

290 295 300

Phe Ala Ser Thr Asn Trp Phe Asn Asn Asn Ala Pro Ser Phe Ser Ala

305 310 315 320

Ile Glu Ala Ala Val Ile Arg Pro Pro His Leu Leu Asp Phe Pro Glu

325 330 335

Gln Leu Thr Ile Phe Ser Val Leu Ser Arg Trp Ser Asn Thr Gln Tyr

340 345 350

Met Asn Tyr Trp Val Gly His Arg Leu Glu Ser Arg Thr Ile Arg Gly

355 360 365

Ser Leu Ser Thr Ser Thr His Gly Asn Thr Asn Thr Ser Ile Asn Pro

370 375 380

Val Thr Leu Gln Phe Thr Ser Arg Asp Val Tyr Arg Thr Glu Ser Phe

385 390 395 400

Ala Gly Ile Asn Ile Leu Leu Thr Thr Pro Val Asn Gly Val Pro Trp

405 410 415

Ala Arg Phe Asn Trp Arg Asn Pro Leu Asn Ser Leu Arg Gly Ser Leu

420 425 430

Leu Tyr Thr Ile Gly Tyr Thr Gly Val Gly Thr Gln Leu Phe Asp Ser

435 440 445

Glu Thr Glu Leu Pro Pro Glu Thr Thr Glu Arg Pro Asn Tyr Glu Ser

450 455 460

Tyr Ser His Arg Leu Ser Asn Ile Arg Leu Ile Ser Gly Asn Thr Leu

465 470 475 480

Arg Ala Pro Val Tyr Ser Trp Thr His Arg Ser Ala Asp Arg Thr Asn

485 490 495

Thr Ile Ser Ser Asp Ser Ile Thr Gln Ile Pro Leu Val Lys Ala His

500 505 510

Thr Leu Gln Ser Gly Thr Thr Val Val Lys Gly Pro Gly Phe Thr Gly

515 520 525

Gly Asp Ile Leu Arg Arg Thr Ser Gly Gly Pro Phe Ala Phe Ser Asn

530 535 540

Val Asn Leu Asp Phe Asn Leu Ser Gln Arg Tyr Arg Ala Arg Ile Arg

545 550 555 560

Tyr Ala Ser Thr Thr Asn Leu Arg Ile Tyr Val Thr Val Ala Gly Glu

565 570 575

Arg Ile Phe Ala Gly Gln Phe Asp Lys Thr Met Asp Ala Gly Ala Pro

580 585 590

Leu Thr Phe Gln Ser Phe Ser Tyr Ala Thr Ile Asn Thr Ala Phe Thr

595 600 605

Phe Pro Glu Arg Ser Ser Ser Leu Thr Val Gly Ala Asp Thr Phe Ser

610 615 620

Ser Gly Asn Glu Val Tyr Val Asp Arg Phe Glu Leu Ile Pro Val Thr

625 630 635 640

Ala Thr Asn Pro Thr Arg Glu Ala Glu Glu Asp Leu Glu Ala Ala Lys

645 650 655

Lys Ala Val Ala Ser Leu Phe Thr Arg Thr Arg Asp Gly Leu Gln Val

660 665 670

Asn Val Thr Asp Tyr Gln Val Asp Gln Ala Ala Asn Leu Val Ser Cys

675 680 685

Leu Ser Asp Glu Gln Tyr Gly His Asp Lys Lys Met Leu Leu Glu Ala

690 695 700

Val Arg Ala Ala Lys Arg Leu Ser Arg Glu Arg Asn Leu Leu Gln Asp

705 710 715 720

Pro Asp Phe Asn Thr Ile Asn Ser Thr Glu Glu Asn Gly Trp Lys Ala

725 730 735

Ser Asn Gly Val Thr Ile Ser Glu Gly Gly Pro Phe Tyr Lys Gly Arg

740 745 750

Ala Leu Gln Leu Ala Ser Ala Arg Glu Asn Tyr Pro Thr Tyr Ile Tyr

755 760 765

Gln Lys Val Asn Ala Ser Glu Leu Lys Pro Tyr Thr Arg Tyr Arg Leu

770 775 780

Asp Gly Phe Val Lys Ser Ser Gln Asp Leu Glu Ile Asp Leu Ile His

785 790 795 800

His His Lys Val His Leu Val Lys Asn Val Pro Asp Asn Leu Val Ser

805 810 815

Asp Thr Tyr Ser Asp Gly Ser Cys Ser Gly Met Asn Arg Cys Glu Glu

820 825 830

Gln Gln Met Val Asn Ala Gln Leu Glu Thr Glu His His His Pro Met

835 840 845

Asp Cys Cys Glu Ala Ala Gln Thr His Glu Phe Ser Ser Tyr Ile Asn

850 855 860

Thr Gly Asp Leu Asn Ser Ser Val Asp Gln Gly Ile Trp Val Val Leu

865 870 875 880

Lys Val Arg Thr Thr Asp Gly Tyr Ala Thr Leu Gly Asn Leu Glu Leu

885 890 895

Val Glu Val Gly Pro Leu Ser Gly Glu Ser Leu Glu Arg Glu Gln Arg

900 905 910

Asp Asn Ala Lys Trp Ser Ala Glu Leu Gly Arg Lys Arg Ala Glu Thr

915 920 925

Asp Arg Val Tyr Gln Asp Ala Lys Gln Ser Ile Asn His Leu Phe Val

930 935 940

Asp Tyr Gln Asp Gln Gln Leu Asn Pro Glu Ile Gly Met Ala Asp Ile

945 950 955 960

Ile Asp Ala Gln Asn Leu Val Ala Ser Ile Ser Asp Val Tyr Ser Asp

965 970 975

Ala Val Leu Gln Ile Pro Gly Ile Asn Tyr Glu Ile Tyr Thr Glu Leu

980 985 990

Ser Asn Arg Leu Gln Gln Ala Ser Tyr Leu Tyr Thr Ser Arg Asn Ala

995 1000 1005

Val Gln Asn Gly Asp Phe Asn Ser Gly Leu Asp Ser Trp Asn Ala

1010 1015 1020

Thr Gly Gly Ala Thr Val Gln Gln Asp Gly Asn Thr His Phe Leu

1025 1030 1035

Val Leu Ser His Trp Asp Ala Gln Val Ser Gln Gln Phe Arg Val

1040 1045 1050

Gln Pro Asn Cys Lys Tyr Val Leu Arg Val Thr Ala Glu Lys Val

1055 1060 1065

Gly Gly Gly Asp Gly Tyr Val Thr Ile Arg Asp Gly Ala His His

1070 1075 1080

Thr Glu Lys Leu Thr Phe Asn Ala Cys Asp Tyr Asp Ile Asn Gly

1085 1090 1095

Thr Tyr Val Thr Asp Asn Thr Tyr Leu Thr Lys Glu Val Val Phe

1100 1105 1110

Tyr Ser His Thr Glu His Met Trp Val Glu Val Ser Glu Thr Glu

1115 1120 1125

Gly Ala Phe His Ile Asp Ser Ile Glu Phe Val Glu Thr Glu Lys

1130 1135 1140

<210> 20

<211> 3696

<212> DNA

<213> 人工的

<220>

<223> 设计用于在植物细胞中表达的编码TIC867_23的合成核苷酸序列。

<400> 20

atgaccagca accgaaagaa cgagaacgag atcatcaacg ccctgtccat accggccgtg 60

tcaaaccact ccgcccagat gaacctctcc accgacgcga ggatcgagga ctccctctgc 120

atcgccgagg gcaacaacat cgacccgttc gtgtctgcaa gcacggtcca gaccggcatc 180

aacatcgcgg gccgcatcct gggcgtgctc ggcgtgccct tcgcgggtca aatcgcctct 240

ttctactcat tcctcgtggg cgagctgtgg ccgcgcggac gtgacccgtg ggaaatcttc 300

ctggagcacg ttgagcagct catccggcag caagtgaccg agaacaccag ggacaccgca 360

ctggcacggc tccagggcct tggcaacagc ttccgcgcct accagcagtc gctggaggac 420

tggctggaga accgagacga cgccagaacc cgctcagttc tgtacacaca gtacatcgcc 480

ctagagctgg acttcctcaa cgctatgccg ctcttcgcca tccgtaacca ggaagtaccg 540

cttctgatgg tgtacgcaca agcagcgaac ctccatctgc tcctgctgcg agacgcatct 600

ctgttcggca gtgagttcgg gctgacgagc caggagatcc agcgctacta cgagcgccaa 660

gtggagaaga ctcgtgagta cagcgactac tgcgcgcgct ggtacaacac gggcttgaac 720

aaccttcgcg ggacaaacgc cgaatcctgg cttcgctaca accagttccg ccgcgacctc 780

acgctgggtg tgctggacct ggtcgcgctc ttcccgtcct acgacacacg ggtgtaccca 840

atgaacacga gcgcacagct cacccgtgag atctacacag atcccatcgg ccgcaccaac 900

gctcccagtg gcttcgcaag cacgaattgg ttcaacaata acgctccttc tttctctgcc 960

atcgaggccg ctgtcatcag accgccgcac ttactcgatt tcccggagca gctcactatc 1020

ttctctgtgt tgtcccggtg gtcgaacacg cagtacatga actactgggt gggccacagg 1080

ctagagagcc ggaccatccg tggcagtctc tcaacctcga cccacggcaa cacgaacacg 1140

agcatcaacc ctgtcactct ccagtttaca tctagggacg tttacaggac agagtcgttc 1200

gctggcatta acattctgtt gaccactccg gtgaacggcg tcccttgggc ccgcttcaac 1260

tggaggaatc ctctgaactc actgcgcggc agccttctct acactatcgg ctacaccggc 1320

gttgggacgc aactcttcga ctcggagacc gagctgccgc ccgagaccac cgagcggcct 1380

aactacgaga gttattcaca caggctctcc aacatccgct tgatttctgg gaacaccttg 1440

cgggctccgg tgtactcctg gacgcaccgc agcgccgaca gaactaatac catcagctcc 1500

gactcgatca cccagatccc gctggtgaag gctcacacgc ttcagtcggg caccacagtc 1560

gtcaagggcc ctggcttcac cggcggcgac atcctgcgtc gcacatctgg cggacccttc 1620

gccttcagca acgtgaactt ggacttcaat ttgtcacagc ggtatcgtgc cagaatccgg 1680

tacgccagca ctacgaacct gcgaatctat gttactgtgg cgggcgagcg gatcttcgcc 1740

gggcaattcg acaagacgat ggacgcggga gcacctctga cattccagtc attctcttac 1800

gccacgatca acacggcatt cacgtttccg gagcgttcca gtagcctgac cgtgggcgct 1860

gataccttca gtagcgggaa cgaggtgtac gttgaccgtt tcgagctgat cccggtcacc 1920

gccaccacgg cgaccttcga ggcggagtat gacttggagc gggctcagga ggccgtcaac 1980

gcgctgttca caaacaccaa tcctcgccgc ctcaagacgg gtgtgactga ttaccacatt 2040

gacgaggtct ccaacttggt cgcgtgtctg tccgatgagt tctgcctgga cgagaagcgg 2100

gaactgctgg agaaggtcaa gtacgccaag cgcctctccg acgaaaggaa cctcctccaa 2160

gatcccaact ttacttccat taacaagcag ccggacttca tctccaccaa cgagcagtcc 2220

aacttcacct caatccacga gcagtcggag cacgggtggt ggggcagcga gaacatcacc 2280

atccaagagg gcaacgacgt cttcaaggag aactacgtga tcctgcccgg caccttcaac 2340

gagtgttacc cgacctatct ctaccagaag attggcgaag cggaactcaa ggcttacacc 2400

cgttaccaac tgagtggcta cattgaggac tcacaagacc tggaaatcta cctgatccgc 2460

tacaacgcca agcacgagac cctcgacgtg cctggcacgg agtccgtctg gcccttgagc 2520

gtggagtctc ctatcggtcg ttgcggcgag cccaatcgct gcgctccgca ctttgagtgg 2580

aatcctgatt tggattgctc ctgccgagac ggtgagaaat gcgcccacca ctcgcaccac 2640

ttcagcctag acatcgacgt gggctgcatc gacctgcacg agaacttggg cgtctgggtc 2700

gtgttcaaga tcaagacaca ggagggccat gctcggcttg ggaacctgga gttcatcgag 2760

gagaagccac tgctgggtga agccttgtca cgggtgaaac gcgccgagaa gaagtggcgg 2820

gacaaacggg agaagctcca gttggagaca aagcgtgtgt acacagaggc caaggaggcc 2880

gtggatgcct tgttcgtgga cagtcagtac gacaggctgc aagcggacac caacatcggg 2940

atgatccacg cggctgataa gcttgttcac agaatccgcg aggcgtacct gtcagagctt 3000

agcgtgatcc caggcgtcaa cgccgaaatc ttcgaggaac tggagggccg cattatcacg 3060

gcaatctcac tttatgacgc gaggaatgtg gtcaagaacg gtgacttcaa caacggcttg 3120

gcgtgttgga acgttaaagg gcacgtggat gtacaacagt cacaccacag aagtgtcttg 3180

gtcatcccgg agtgggaggc ggaagtgagc caggccgtcc gggtctgccc tgggcgcggt 3240

tacatcctcc gcgtgacagc gtacaaggag ggctacggtg agggctgcgt gacgatccac 3300

gagattgaga acaacacgga cgagcttaag ttcaagaact gcgaggagga ggaagtgtac 3360

ccgacagaca ccggcacctg caacgactac accgcccacc aagggaccgc cgcctgcaac 3420

agccgcaacg cgggctatga agatgcgtac gaggttgata ccaccgcctc agtgaactac 3480

aaaccgactt atgaggagga gacatacacg gacgtcaggc gcgacaacca ttgtgagtac 3540

gaccgtggct acgtgaacta tccgccggtg ccagcgggct acatgacgaa ggagctagaa 3600

tacttccctg agacggacaa ggtgtggatt gaaatcggcg agaccgaggg caagtttatc 3660

gtggattctg tcgagctgct gctaatggag gagtag 3696

<210> 21

<211> 1231

<212> PRT

<213> 人工的

<220>

<223> 嵌合蛋白质变体TIC867_23的氨基酸序列。

<400> 21

Met Thr Ser Asn Arg Lys Asn Glu Asn Glu Ile Ile Asn Ala Leu Ser

1 5 10 15

Ile Pro Ala Val Ser Asn His Ser Ala Gln Met Asn Leu Ser Thr Asp

20 25 30

Ala Arg Ile Glu Asp Ser Leu Cys Ile Ala Glu Gly Asn Asn Ile Asp

35 40 45

Pro Phe Val Ser Ala Ser Thr Val Gln Thr Gly Ile Asn Ile Ala Gly

50 55 60

Arg Ile Leu Gly Val Leu Gly Val Pro Phe Ala Gly Gln Ile Ala Ser

65 70 75 80

Phe Tyr Ser Phe Leu Val Gly Glu Leu Trp Pro Arg Gly Arg Asp Pro

85 90 95

Trp Glu Ile Phe Leu Glu His Val Glu Gln Leu Ile Arg Gln Gln Val

100 105 110

Thr Glu Asn Thr Arg Asp Thr Ala Leu Ala Arg Leu Gln Gly Leu Gly

115 120 125

Asn Ser Phe Arg Ala Tyr Gln Gln Ser Leu Glu Asp Trp Leu Glu Asn

130 135 140

Arg Asp Asp Ala Arg Thr Arg Ser Val Leu Tyr Thr Gln Tyr Ile Ala

145 150 155 160

Leu Glu Leu Asp Phe Leu Asn Ala Met Pro Leu Phe Ala Ile Arg Asn

165 170 175

Gln Glu Val Pro Leu Leu Met Val Tyr Ala Gln Ala Ala Asn Leu His

180 185 190

Leu Leu Leu Leu Arg Asp Ala Ser Leu Phe Gly Ser Glu Phe Gly Leu

195 200 205

Thr Ser Gln Glu Ile Gln Arg Tyr Tyr Glu Arg Gln Val Glu Lys Thr

210 215 220

Arg Glu Tyr Ser Asp Tyr Cys Ala Arg Trp Tyr Asn Thr Gly Leu Asn

225 230 235 240

Asn Leu Arg Gly Thr Asn Ala Glu Ser Trp Leu Arg Tyr Asn Gln Phe

245 250 255

Arg Arg Asp Leu Thr Leu Gly Val Leu Asp Leu Val Ala Leu Phe Pro

260 265 270

Ser Tyr Asp Thr Arg Val Tyr Pro Met Asn Thr Ser Ala Gln Leu Thr

275 280 285

Arg Glu Ile Tyr Thr Asp Pro Ile Gly Arg Thr Asn Ala Pro Ser Gly

290 295 300

Phe Ala Ser Thr Asn Trp Phe Asn Asn Asn Ala Pro Ser Phe Ser Ala

305 310 315 320

Ile Glu Ala Ala Val Ile Arg Pro Pro His Leu Leu Asp Phe Pro Glu

325 330 335

Gln Leu Thr Ile Phe Ser Val Leu Ser Arg Trp Ser Asn Thr Gln Tyr

340 345 350

Met Asn Tyr Trp Val Gly His Arg Leu Glu Ser Arg Thr Ile Arg Gly

355 360 365

Ser Leu Ser Thr Ser Thr His Gly Asn Thr Asn Thr Ser Ile Asn Pro

370 375 380

Val Thr Leu Gln Phe Thr Ser Arg Asp Val Tyr Arg Thr Glu Ser Phe

385 390 395 400

Ala Gly Ile Asn Ile Leu Leu Thr Thr Pro Val Asn Gly Val Pro Trp

405 410 415

Ala Arg Phe Asn Trp Arg Asn Pro Leu Asn Ser Leu Arg Gly Ser Leu

420 425 430

Leu Tyr Thr Ile Gly Tyr Thr Gly Val Gly Thr Gln Leu Phe Asp Ser

435 440 445

Glu Thr Glu Leu Pro Pro Glu Thr Thr Glu Arg Pro Asn Tyr Glu Ser

450 455 460

Tyr Ser His Arg Leu Ser Asn Ile Arg Leu Ile Ser Gly Asn Thr Leu

465 470 475 480

Arg Ala Pro Val Tyr Ser Trp Thr His Arg Ser Ala Asp Arg Thr Asn

485 490 495

Thr Ile Ser Ser Asp Ser Ile Thr Gln Ile Pro Leu Val Lys Ala His

500 505 510

Thr Leu Gln Ser Gly Thr Thr Val Val Lys Gly Pro Gly Phe Thr Gly

515 520 525

Gly Asp Ile Leu Arg Arg Thr Ser Gly Gly Pro Phe Ala Phe Ser Asn

530 535 540

Val Asn Leu Asp Phe Asn Leu Ser Gln Arg Tyr Arg Ala Arg Ile Arg

545 550 555 560

Tyr Ala Ser Thr Thr Asn Leu Arg Ile Tyr Val Thr Val Ala Gly Glu

565 570 575

Arg Ile Phe Ala Gly Gln Phe Asp Lys Thr Met Asp Ala Gly Ala Pro

580 585 590

Leu Thr Phe Gln Ser Phe Ser Tyr Ala Thr Ile Asn Thr Ala Phe Thr

595 600 605

Phe Pro Glu Arg Ser Ser Ser Leu Thr Val Gly Ala Asp Thr Phe Ser

610 615 620

Ser Gly Asn Glu Val Tyr Val Asp Arg Phe Glu Leu Ile Pro Val Thr

625 630 635 640

Ala Thr Thr Ala Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln

645 650 655

Glu Ala Val Asn Ala Leu Phe Thr Asn Thr Asn Pro Arg Arg Leu Lys

660 665 670

Thr Gly Val Thr Asp Tyr His Ile Asp Glu Val Ser Asn Leu Val Ala

675 680 685

Cys Leu Ser Asp Glu Phe Cys Leu Asp Glu Lys Arg Glu Leu Leu Glu

690 695 700

Lys Val Lys Tyr Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln

705 710 715 720

Asp Pro Asn Phe Thr Ser Ile Asn Lys Gln Pro Asp Phe Ile Ser Thr

725 730 735

Asn Glu Gln Ser Asn Phe Thr Ser Ile His Glu Gln Ser Glu His Gly

740 745 750

Trp Trp Gly Ser Glu Asn Ile Thr Ile Gln Glu Gly Asn Asp Val Phe

755 760 765

Lys Glu Asn Tyr Val Ile Leu Pro Gly Thr Phe Asn Glu Cys Tyr Pro

770 775 780

Thr Tyr Leu Tyr Gln Lys Ile Gly Glu Ala Glu Leu Lys Ala Tyr Thr

785 790 795 800

Arg Tyr Gln Leu Ser Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile

805 810 815

Tyr Leu Ile Arg Tyr Asn Ala Lys His Glu Thr Leu Asp Val Pro Gly

820 825 830

Thr Glu Ser Val Trp Pro Leu Ser Val Glu Ser Pro Ile Gly Arg Cys

835 840 845

Gly Glu Pro Asn Arg Cys Ala Pro His Phe Glu Trp Asn Pro Asp Leu

850 855 860

Asp Cys Ser Cys Arg Asp Gly Glu Lys Cys Ala His His Ser His His

865 870 875 880

Phe Ser Leu Asp Ile Asp Val Gly Cys Ile Asp Leu His Glu Asn Leu

885 890 895

Gly Val Trp Val Val Phe Lys Ile Lys Thr Gln Glu Gly His Ala Arg

900 905 910

Leu Gly Asn Leu Glu Phe Ile Glu Glu Lys Pro Leu Leu Gly Glu Ala

915 920 925

Leu Ser Arg Val Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu

930 935 940

Lys Leu Gln Leu Glu Thr Lys Arg Val Tyr Thr Glu Ala Lys Glu Ala

945 950 955 960

Val Asp Ala Leu Phe Val Asp Ser Gln Tyr Asp Arg Leu Gln Ala Asp

965 970 975

Thr Asn Ile Gly Met Ile His Ala Ala Asp Lys Leu Val His Arg Ile

980 985 990

Arg Glu Ala Tyr Leu Ser Glu Leu Ser Val Ile Pro Gly Val Asn Ala

995 1000 1005

Glu Ile Phe Glu Glu Leu Glu Gly Arg Ile Ile Thr Ala Ile Ser

1010 1015 1020

Leu Tyr Asp Ala Arg Asn Val Val Lys Asn Gly Asp Phe Asn Asn

1025 1030 1035

Gly Leu Ala Cys Trp Asn Val Lys Gly His Val Asp Val Gln Gln

1040 1045 1050

Ser His His Arg Ser Val Leu Val Ile Pro Glu Trp Glu Ala Glu

1055 1060 1065

Val Ser Gln Ala Val Arg Val Cys Pro Gly Arg Gly Tyr Ile Leu

1070 1075 1080

Arg Val Thr Ala Tyr Lys Glu Gly Tyr Gly Glu Gly Cys Val Thr

1085 1090 1095

Ile His Glu Ile Glu Asn Asn Thr Asp Glu Leu Lys Phe Lys Asn

1100 1105 1110

Cys Glu Glu Glu Glu Val Tyr Pro Thr Asp Thr Gly Thr Cys Asn

1115 1120 1125

Asp Tyr Thr Ala His Gln Gly Thr Ala Ala Cys Asn Ser Arg Asn

1130 1135 1140

Ala Gly Tyr Glu Asp Ala Tyr Glu Val Asp Thr Thr Ala Ser Val

1145 1150 1155

Asn Tyr Lys Pro Thr Tyr Glu Glu Glu Thr Tyr Thr Asp Val Arg

1160 1165 1170

Arg Asp Asn His Cys Glu Tyr Asp Arg Gly Tyr Val Asn Tyr Pro

1175 1180 1185

Pro Val Pro Ala Gly Tyr Met Thr Lys Glu Leu Glu Tyr Phe Pro

1190 1195 1200

Glu Thr Asp Lys Val Trp Ile Glu Ile Gly Glu Thr Glu Gly Lys

1205 1210 1215

Phe Ile Val Asp Ser Val Glu Leu Leu Leu Met Glu Glu

1220 1225 1230

<210> 22

<211> 3666

<212> DNA

<213> 人工的

<220>

<223> 设计用于在植物细胞中表达的编码TIC867_24的合成核苷酸序列。

<400> 22

atgaccagca accgaaagaa cgagaacgag atcatcaacg ccctgtccat accggccgtg 60

tcaaaccact ccgcccagat gaacctctcc accgacgcga ggatcgagga ctccctctgc 120

atcgccgagg gcaacaacat cgacccgttc gtgtctgcaa gcacggtcca gaccggcatc 180

aacatcgcgg gccgcatcct gggcgtgctc ggcgtgccct tcgcgggtca aatcgcctct 240

ttctactcat tcctcgtggg cgagctgtgg ccgcgcggac gtgacccgtg ggaaatcttc 300

ctggagcacg ttgagcagct catccggcag caagtgaccg agaacaccag ggacaccgca 360

ctggcacggc tccagggcct tggcaacagc ttccgcgcct accagcagtc gctggaggac 420

tggctggaga accgagacga cgccagaacc cgctcagttc tgtacacaca gtacatcgcc 480

ctagagctgg acttcctcaa cgctatgccg ctcttcgcca tccgtaacca ggaagtaccg 540

cttctgatgg tgtacgcaca agcagcgaac ctccatctgc tcctgctgcg agacgcatct 600

ctgttcggca gtgagttcgg gctgacgagc caggagatcc agcgctacta cgagcgccaa 660

gtggagaaga ctcgtgagta cagcgactac tgcgcgcgct ggtacaacac gggcttgaac 720

aaccttcgcg ggacaaacgc cgaatcctgg cttcgctaca accagttccg ccgcgacctc 780

acgctgggtg tgctggacct ggtcgcgctc ttcccgtcct acgacacacg ggtgtaccca 840

atgaacacga gcgcacagct cacccgtgag atctacacag atcccatcgg ccgcaccaac 900

gctcccagtg gcttcgcaag cacgaattgg ttcaacaata acgctccttc tttctctgcc 960

atcgaggccg ctgtcatcag accgccgcac ttactcgatt tcccggagca gctcactatc 1020

ttctctgtgt tgtcccggtg gtcgaacacg cagtacatga actactgggt gggccacagg 1080

ctagagagcc ggaccatccg tggcagtctc tcaacctcga cccacggcaa cacgaacacg 1140

agcatcaacc ctgtcactct ccagtttaca tctagggacg tttacaggac agagtcgttc 1200

gctggcatta acattctgtt gaccactccg gtgaacggcg tcccttgggc ccgcttcaac 1260

tggaggaatc ctctgaactc actgcgcggc agccttctct acactatcgg ctacaccggc 1320

gttgggacgc aactcttcga ctcggagacc gagctgccgc ccgagaccac cgagcggcct 1380

aactacgaga gttattcaca caggctctcc aacatccgct tgatttctgg gaacaccttg 1440

cgggctccgg tgtactcctg gacgcaccgc agcgccgaca gaactaatac catcagctcc 1500

gactcgatca cccagatccc gctggtgaag gctcacacgc ttcagtcggg caccacagtc 1560

gtcaagggcc ctggcttcac cggcggcgac atcctgcgtc gcacatctgg cggacccttc 1620

gccttcagca acgtgaactt ggacttcaat ttgtcacagc ggtatcgtgc cagaatccgg 1680

tacgccagca ctacgaacct gcgaatctat gttactgtgg cgggcgagcg gatcttcgcc 1740

gggcaattcg acaagacgat ggacgcggga gcacctctga cattccagtc attctcttac 1800

gccacgatca acacggcatt cacgtttccg gagcgttcca gtagcctgac cgtgggcgct 1860

gataccttca gtagcgggaa cgaggtgtac gttgaccgtt tcgagctgat cccggtcacc 1920

gccaccaccg cgacgtttga agctgaatcc gacctcgagc gtgcgcgcaa ggcggtgaac 1980

gctctgttca cgagcaccaa ccctcgtggc ttgaagacgg atgtgacgga ctaccacatc 2040

gaccaagtct cgaacctcgt ggagtgcctg agcgacgagt tctgtcttga caagaagcgc 2100

gagctgctgg aggaggtgaa gtacgccaag cgcctctccg atgagcgcaa cctgctccaa 2160

gatcctacct tcacgtcgat ttccggccaa accgaccgtg gatggatcgg ctcgactggc 2220

atctccatcc agggcggcga cgacatcttc aaggagaact atgttcggct gccgggcacg 2280

gtggacgagt gttacccgac gtacctctac cagaagatag acgagagtca actcaagtcc 2340

tacacgcggt atcagttacg tggctacatt gaagactccc aggacttgga aatctatctc 2400

atacggtaca acgccaagca cgagacctta agcgtgccgg gaacggagtc gccctggcca 2460

agctctggcg tgtacccttc cggtaggtgc ggcgagccca accgctgtgc acctcgaatc 2520

gaatggaacc cggaccttga ctgctcttgc cggtacggcg agaagtgcgt ccatcattct 2580

caccacttca gcttggacat tgacgtcggc tgcaccgacc tcaatgaaga cctcggagtg 2640

tgggtcatct tcaagatcaa gacacaggac gggcacgcga aactaggaaa cctggagttc 2700

atcgaggaga agccactcct cggcaaggca ctttccaggg tcaagcgggc cgagaagaaa 2760

tggagggaca agtacgagaa actccagctc gaaacaaagc gggtgtacac ggaggcaaag 2820

gaatccgtgg acgccctgtt cgtggactct cagtacgaca agctccaggc gaacacaaac 2880

attggcatca tccacggtgc ggacaagcaa gtgcacagga tacgggagcc ttacctctcg 2940

gagctgccgg tgattccctc gatcaacgcg gcgatcttcg aggaactgga gggccacatc 3000

ttcaaggcgt attctctgta cgacgcgcgt aacgtcatca agaacggcga cttcaacaat 3060

gggctgtcct gctggaacgt taaaggccac gtcgatgtcc agcagaacca ccataggtca 3120

gtcctggtgc tgagcgagtg ggaggcggag gtgtcccaga aggtgcgcgt gtgcccggat 3180

cgcggctaca tcttgagggt gacagcctac aaggagggct acggcgaggg ctgtgtcacg 3240

atccatgagt tcgaggacaa cacggatgtc ctgaaattcc gtaacttcgt cgaggaggag 3300

gtctatccca acaacaccgt gacctgcaac gactacacga ccaatcagtc ggctgagggc 3360

agtaccgatg cctgcaacag ctacaaccgt ggttacgaag atggatacga gaaccgctac 3420

gagcccaatc cttcggctcc cgtgaattac actcccacgt acgaggaggg catgtacact 3480

gacactcagg gctacaacca ttgcgtcagc gaccgtggct accgcaacca cacgccgctc 3540

ccagcgggct acgtgacgct ggagctggaa tactttcccg agacagaaca agtgtggata 3600

gagatcggcg agaccgaggg cacattcatc gtgggctctg tggaattgct cctcatggag 3660

gagtaa 3666

<210> 23

<211> 1221

<212> PRT

<213> 人工的

<220>

<223> 嵌合蛋白质变体TIC867_24的氨基酸序列。

<400> 23

Met Thr Ser Asn Arg Lys Asn Glu Asn Glu Ile Ile Asn Ala Leu Ser

1 5 10 15

Ile Pro Ala Val Ser Asn His Ser Ala Gln Met Asn Leu Ser Thr Asp

20 25 30

Ala Arg Ile Glu Asp Ser Leu Cys Ile Ala Glu Gly Asn Asn Ile Asp

35 40 45

Pro Phe Val Ser Ala Ser Thr Val Gln Thr Gly Ile Asn Ile Ala Gly

50 55 60

Arg Ile Leu Gly Val Leu Gly Val Pro Phe Ala Gly Gln Ile Ala Ser

65 70 75 80

Phe Tyr Ser Phe Leu Val Gly Glu Leu Trp Pro Arg Gly Arg Asp Pro

85 90 95

Trp Glu Ile Phe Leu Glu His Val Glu Gln Leu Ile Arg Gln Gln Val

100 105 110

Thr Glu Asn Thr Arg Asp Thr Ala Leu Ala Arg Leu Gln Gly Leu Gly

115 120 125

Asn Ser Phe Arg Ala Tyr Gln Gln Ser Leu Glu Asp Trp Leu Glu Asn

130 135 140

Arg Asp Asp Ala Arg Thr Arg Ser Val Leu Tyr Thr Gln Tyr Ile Ala

145 150 155 160

Leu Glu Leu Asp Phe Leu Asn Ala Met Pro Leu Phe Ala Ile Arg Asn

165 170 175

Gln Glu Val Pro Leu Leu Met Val Tyr Ala Gln Ala Ala Asn Leu His

180 185 190

Leu Leu Leu Leu Arg Asp Ala Ser Leu Phe Gly Ser Glu Phe Gly Leu

195 200 205

Thr Ser Gln Glu Ile Gln Arg Tyr Tyr Glu Arg Gln Val Glu Lys Thr

210 215 220

Arg Glu Tyr Ser Asp Tyr Cys Ala Arg Trp Tyr Asn Thr Gly Leu Asn

225 230 235 240

Asn Leu Arg Gly Thr Asn Ala Glu Ser Trp Leu Arg Tyr Asn Gln Phe

245 250 255

Arg Arg Asp Leu Thr Leu Gly Val Leu Asp Leu Val Ala Leu Phe Pro

260 265 270

Ser Tyr Asp Thr Arg Val Tyr Pro Met Asn Thr Ser Ala Gln Leu Thr

275 280 285

Arg Glu Ile Tyr Thr Asp Pro Ile Gly Arg Thr Asn Ala Pro Ser Gly

290 295 300

Phe Ala Ser Thr Asn Trp Phe Asn Asn Asn Ala Pro Ser Phe Ser Ala

305 310 315 320

Ile Glu Ala Ala Val Ile Arg Pro Pro His Leu Leu Asp Phe Pro Glu

325 330 335

Gln Leu Thr Ile Phe Ser Val Leu Ser Arg Trp Ser Asn Thr Gln Tyr

340 345 350

Met Asn Tyr Trp Val Gly His Arg Leu Glu Ser Arg Thr Ile Arg Gly

355 360 365

Ser Leu Ser Thr Ser Thr His Gly Asn Thr Asn Thr Ser Ile Asn Pro

370 375 380

Val Thr Leu Gln Phe Thr Ser Arg Asp Val Tyr Arg Thr Glu Ser Phe

385 390 395 400

Ala Gly Ile Asn Ile Leu Leu Thr Thr Pro Val Asn Gly Val Pro Trp

405 410 415

Ala Arg Phe Asn Trp Arg Asn Pro Leu Asn Ser Leu Arg Gly Ser Leu

420 425 430

Leu Tyr Thr Ile Gly Tyr Thr Gly Val Gly Thr Gln Leu Phe Asp Ser

435 440 445

Glu Thr Glu Leu Pro Pro Glu Thr Thr Glu Arg Pro Asn Tyr Glu Ser

450 455 460

Tyr Ser His Arg Leu Ser Asn Ile Arg Leu Ile Ser Gly Asn Thr Leu

465 470 475 480

Arg Ala Pro Val Tyr Ser Trp Thr His Arg Ser Ala Asp Arg Thr Asn

485 490 495

Thr Ile Ser Ser Asp Ser Ile Thr Gln Ile Pro Leu Val Lys Ala His

500 505 510

Thr Leu Gln Ser Gly Thr Thr Val Val Lys Gly Pro Gly Phe Thr Gly

515 520 525

Gly Asp Ile Leu Arg Arg Thr Ser Gly Gly Pro Phe Ala Phe Ser Asn

530 535 540

Val Asn Leu Asp Phe Asn Leu Ser Gln Arg Tyr Arg Ala Arg Ile Arg

545 550 555 560

Tyr Ala Ser Thr Thr Asn Leu Arg Ile Tyr Val Thr Val Ala Gly Glu

565 570 575

Arg Ile Phe Ala Gly Gln Phe Asp Lys Thr Met Asp Ala Gly Ala Pro

580 585 590

Leu Thr Phe Gln Ser Phe Ser Tyr Ala Thr Ile Asn Thr Ala Phe Thr

595 600 605

Phe Pro Glu Arg Ser Ser Ser Leu Thr Val Gly Ala Asp Thr Phe Ser

610 615 620

Ser Gly Asn Glu Val Tyr Val Asp Arg Phe Glu Leu Ile Pro Val Thr

625 630 635 640

Ala Thr Thr Ala Thr Phe Glu Ala Glu Ser Asp Leu Glu Arg Ala Arg

645 650 655

Lys Ala Val Asn Ala Leu Phe Thr Ser Thr Asn Pro Arg Gly Leu Lys

660 665 670

Thr Asp Val Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Glu

675 680 685

Cys Leu Ser Asp Glu Phe Cys Leu Asp Lys Lys Arg Glu Leu Leu Glu

690 695 700

Glu Val Lys Tyr Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln

705 710 715 720

Asp Pro Thr Phe Thr Ser Ile Ser Gly Gln Thr Asp Arg Gly Trp Ile

725 730 735

Gly Ser Thr Gly Ile Ser Ile Gln Gly Gly Asp Asp Ile Phe Lys Glu

740 745 750

Asn Tyr Val Arg Leu Pro Gly Thr Val Asp Glu Cys Tyr Pro Thr Tyr

755 760 765

Leu Tyr Gln Lys Ile Asp Glu Ser Gln Leu Lys Ser Tyr Thr Arg Tyr

770 775 780

Gln Leu Arg Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu

785 790 795 800

Ile Arg Tyr Asn Ala Lys His Glu Thr Leu Ser Val Pro Gly Thr Glu

805 810 815

Ser Pro Trp Pro Ser Ser Gly Val Tyr Pro Ser Gly Arg Cys Gly Glu

820 825 830

Pro Asn Arg Cys Ala Pro Arg Ile Glu Trp Asn Pro Asp Leu Asp Cys

835 840 845

Ser Cys Arg Tyr Gly Glu Lys Cys Val His His Ser His His Phe Ser

850 855 860

Leu Asp Ile Asp Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val

865 870 875 880

Trp Val Ile Phe Lys Ile Lys Thr Gln Asp Gly His Ala Lys Leu Gly

885 890 895

Asn Leu Glu Phe Ile Glu Glu Lys Pro Leu Leu Gly Lys Ala Leu Ser

900 905 910

Arg Val Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys Tyr Glu Lys Leu

915 920 925

Gln Leu Glu Thr Lys Arg Val Tyr Thr Glu Ala Lys Glu Ser Val Asp

930 935 940

Ala Leu Phe Val Asp Ser Gln Tyr Asp Lys Leu Gln Ala Asn Thr Asn

945 950 955 960

Ile Gly Ile Ile His Gly Ala Asp Lys Gln Val His Arg Ile Arg Glu

965 970 975

Pro Tyr Leu Ser Glu Leu Pro Val Ile Pro Ser Ile Asn Ala Ala Ile

980 985 990

Phe Glu Glu Leu Glu Gly His Ile Phe Lys Ala Tyr Ser Leu Tyr Asp

995 1000 1005

Ala Arg Asn Val Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Ser

1010 1015 1020

Cys Trp Asn Val Lys Gly His Val Asp Val Gln Gln Asn His His

1025 1030 1035

Arg Ser Val Leu Val Leu Ser Glu Trp Glu Ala Glu Val Ser Gln

1040 1045 1050

Lys Val Arg Val Cys Pro Asp Arg Gly Tyr Ile Leu Arg Val Thr

1055 1060 1065

Ala Tyr Lys Glu Gly Tyr Gly Glu Gly Cys Val Thr Ile His Glu

1070 1075 1080

Phe Glu Asp Asn Thr Asp Val Leu Lys Phe Arg Asn Phe Val Glu

1085 1090 1095

Glu Glu Val Tyr Pro Asn Asn Thr Val Thr Cys Asn Asp Tyr Thr

1100 1105 1110

Thr Asn Gln Ser Ala Glu Gly Ser Thr Asp Ala Cys Asn Ser Tyr

1115 1120 1125

Asn Arg Gly Tyr Glu Asp Gly Tyr Glu Asn Arg Tyr Glu Pro Asn

1130 1135 1140

Pro Ser Ala Pro Val Asn Tyr Thr Pro Thr Tyr Glu Glu Gly Met

1145 1150 1155

Tyr Thr Asp Thr Gln Gly Tyr Asn His Cys Val Ser Asp Arg Gly

1160 1165 1170

Tyr Arg Asn His Thr Pro Leu Pro Ala Gly Tyr Val Thr Leu Glu

1175 1180 1185

Leu Glu Tyr Phe Pro Glu Thr Glu Gln Val Trp Ile Glu Ile Gly

1190 1195 1200

Glu Thr Glu Gly Thr Phe Ile Val Gly Ser Val Glu Leu Leu Leu

1205 1210 1215

Met Glu Glu

1220

<210> 24

<211> 3651

<212> DNA

<213> 人工的

<220>

<223> 设计用于在植物细胞中表达的编码TIC867_25的合成核苷酸序列。

<400> 24

atgaccagca accgaaagaa cgagaacgag atcatcaacg ccctgtccat accggccgtg 60

tcaaaccact ccgcccagat gaacctctcc accgacgcga ggatcgagga ctccctctgc 120

atcgccgagg gcaacaacat cgacccgttc gtgtctgcaa gcacggtcca gaccggcatc 180

aacatcgcgg gccgcatcct gggcgtgctc ggcgtgccct tcgcgggtca aatcgcctct 240

ttctactcat tcctcgtggg cgagctgtgg ccgcgcggac gtgacccgtg ggaaatcttc 300

ctggagcacg ttgagcagct catccggcag caagtgaccg agaacaccag ggacaccgca 360

ctggcacggc tccagggcct tggcaacagc ttccgcgcct accagcagtc gctggaggac 420

tggctggaga accgagacga cgccagaacc cgctcagttc tgtacacaca gtacatcgcc 480

ctagagctgg acttcctcaa cgctatgccg ctcttcgcca tccgtaacca ggaagtaccg 540

cttctgatgg tgtacgcaca agcagcgaac ctccatctgc tcctgctgcg agacgcatct 600

ctgttcggca gtgagttcgg gctgacgagc caggagatcc agcgctacta cgagcgccaa 660

gtggagaaga ctcgtgagta cagcgactac tgcgcgcgct ggtacaacac gggcttgaac 720

aaccttcgcg ggacaaacgc cgaatcctgg cttcgctaca accagttccg ccgcgacctc 780

acgctgggtg tgctggacct ggtcgcgctc ttcccgtcct acgacacacg ggtgtaccca 840

atgaacacga gcgcacagct cacccgtgag atctacacag atcccatcgg ccgcaccaac 900

gctcccagtg gcttcgcaag cacgaattgg ttcaacaata acgctccttc tttctctgcc 960

atcgaggccg ctgtcatcag accgccgcac ttactcgatt tcccggagca gctcactatc 1020

ttctctgtgt tgtcccggtg gtcgaacacg cagtacatga actactgggt gggccacagg 1080

ctagagagcc ggaccatccg tggcagtctc tcaacctcga cccacggcaa cacgaacacg 1140

agcatcaacc ctgtcactct ccagtttaca tctagggacg tttacaggac agagtcgttc 1200

gctggcatta acattctgtt gaccactccg gtgaacggcg tcccttgggc ccgcttcaac 1260

tggaggaatc ctctgaactc actgcgcggc agccttctct acactatcgg ctacaccggc 1320

gttgggacgc aactcttcga ctcggagacc gagctgccgc ccgagaccac cgagcggcct 1380

aactacgaga gttattcaca caggctctcc aacatccgct tgatttctgg gaacaccttg 1440

cgggctccgg tgtactcctg gacgcaccgc agcgccgaca gaactaatac catcagctcc 1500

gactcgatca cccagatccc gctggtgaag gctcacacgc ttcagtcggg caccacagtc 1560

gtcaagggcc ctggcttcac cggcggcgac atcctgcgtc gcacatctgg cggacccttc 1620

gccttcagca acgtgaactt ggacttcaat ttgtcacagc ggtatcgtgc cagaatccgg 1680

tacgccagca ctacgaacct gcgaatctat gttactgtgg cgggcgagcg gatcttcgcc 1740

gggcaattcg acaagacgat ggacgcggga gcacctctga cattccagtc attctcttac 1800

gccacgatca acacggcatt cacgtttccg gagcgttcca gtagcctgac cgtgggcgct 1860

gataccttca gtagcgggaa cgaggtgtac gttgaccgtt tcgagctgat cccggtcacc 1920

gccaccgatg ctacctttga agcagagtcc gacttggaac gtgcacagaa ggcagtgaac 1980

gcactcttca cctcaagcaa ccagatcgga ttgaagacag atgtgacaga ttaccacatc 2040

gaccaagtga gcaacttggt ggattgcttg tcagatgagt tctgcttgga tgagaagcgt 2100

gaactctccg agaaggtgaa gcacgcaaag cgtctctcag atgaacgtaa tctccttcaa 2160

gaccctaact ttcgtggtat caatcgtcag ccagatcgtg gatggcgtgg atcaacagac 2220

atcaccatcc agggaggcga tgatgtgttc aaggagaact acgtgaccct cccaggaacc 2280

gtggatgaat gctacccaac ctacctctac cagaagatcg acgagtcaaa gctcaaggct 2340

tacacccgtt atgaactccg tggctacatc gaagatagcc aggatctcga aatctatctc 2400

atccgttaca atgctaagca cgaaatcgtg aatgtgccag gaaccggctc actctggcca 2460

ctctcagcac agtcaccaat cggcaagtgc ggcgaaccca atcgctgcgc tcctcatctc 2520

gaatggaatc ccgatctcga ctgctcctgc cgagacggcg agaagtgtgc acatcactca 2580

caccacttca ccctcgacat cgacgtgggc tgcaccgacc tcaatgaaga cctgggcgtg 2640

tgggtgatct tcaagatcaa gacccaggac ggccacgcac gactgggcaa tctggagttt 2700

ctggaggaga agccactgct tggcgaggca ctggcacgag tgaaacgagc cgagaagaaa 2760

tggcgagaca aacgtgagaa gctgcaactg gagaccaaca tcgtgtacaa agaggccaaa 2820

gagtcagttg acgccctgtt tgtcaatagc cagtatgacc gactgcaagt tgacaccaac 2880

atcgccatga tccacgctgc ggacaagcgc gtccaccgca tccgcgaggc ttatctgccc 2940

gagctgagcg tcattcccgg cgtcaatgcc gcgatcttcg aggagttaga gggccgcatc 3000

ttcaccgcct acagcctcta tgacgcccgc aatgtcatta agaatggcga cttcaacaat 3060

ggcttactat gctggaatgt caaagggcac gttgacgtcg aggagcagaa caatcaccgc 3120

agcgtcttag tcatacccga gtgggaggcc gaagtcagcc aggaagtccg cgtctgtcca 3180

gggcgcgggt acatcctgcg ggtcaccgcc tacaaagagg gatacggcga gggttgtgtc 3240

accatacacg agatagagga caataccgac gaactcaagt tcagcaattg tgtcgaggag 3300

gaagtctatc ccaacaatac cgtaacctgc aacaactaca ccggaaccca ggaggagtat 3360

gaagggacgt acacctcgcg gaaccagggc tatgacgaag cctatgggaa caacccgtcg 3420

gtgcctgctg actatgcgtc ggtctatgag gagaaatcgt acacggacgg gcggcgggag 3480

aatccgtgtg agtcgaatcg cgggtatggt gactacacgc cgctaccggc gggctatgta 3540

acgaaagacc tggaatactt cccggagacg gacaaagtat ggatagagat aggcgagacg 3600

gagggaacgt tcatcgtgga ctcggtagag ctgctgctca tggaggagtg a 3651

<210> 25

<211> 1216

<212> PRT

<213> 人工的

<220>

<223> 嵌合蛋白质变体TIC867_25的氨基酸序列。

<400> 25

Met Thr Ser Asn Arg Lys Asn Glu Asn Glu Ile Ile Asn Ala Leu Ser

1 5 10 15

Ile Pro Ala Val Ser Asn His Ser Ala Gln Met Asn Leu Ser Thr Asp

20 25 30

Ala Arg Ile Glu Asp Ser Leu Cys Ile Ala Glu Gly Asn Asn Ile Asp

35 40 45

Pro Phe Val Ser Ala Ser Thr Val Gln Thr Gly Ile Asn Ile Ala Gly

50 55 60

Arg Ile Leu Gly Val Leu Gly Val Pro Phe Ala Gly Gln Ile Ala Ser

65 70 75 80

Phe Tyr Ser Phe Leu Val Gly Glu Leu Trp Pro Arg Gly Arg Asp Pro

85 90 95

Trp Glu Ile Phe Leu Glu His Val Glu Gln Leu Ile Arg Gln Gln Val

100 105 110

Thr Glu Asn Thr Arg Asp Thr Ala Leu Ala Arg Leu Gln Gly Leu Gly

115 120 125

Asn Ser Phe Arg Ala Tyr Gln Gln Ser Leu Glu Asp Trp Leu Glu Asn

130 135 140

Arg Asp Asp Ala Arg Thr Arg Ser Val Leu Tyr Thr Gln Tyr Ile Ala

145 150 155 160

Leu Glu Leu Asp Phe Leu Asn Ala Met Pro Leu Phe Ala Ile Arg Asn

165 170 175

Gln Glu Val Pro Leu Leu Met Val Tyr Ala Gln Ala Ala Asn Leu His

180 185 190

Leu Leu Leu Leu Arg Asp Ala Ser Leu Phe Gly Ser Glu Phe Gly Leu

195 200 205

Thr Ser Gln Glu Ile Gln Arg Tyr Tyr Glu Arg Gln Val Glu Lys Thr

210 215 220

Arg Glu Tyr Ser Asp Tyr Cys Ala Arg Trp Tyr Asn Thr Gly Leu Asn

225 230 235 240

Asn Leu Arg Gly Thr Asn Ala Glu Ser Trp Leu Arg Tyr Asn Gln Phe

245 250 255

Arg Arg Asp Leu Thr Leu Gly Val Leu Asp Leu Val Ala Leu Phe Pro

260 265 270

Ser Tyr Asp Thr Arg Val Tyr Pro Met Asn Thr Ser Ala Gln Leu Thr

275 280 285

Arg Glu Ile Tyr Thr Asp Pro Ile Gly Arg Thr Asn Ala Pro Ser Gly

290 295 300

Phe Ala Ser Thr Asn Trp Phe Asn Asn Asn Ala Pro Ser Phe Ser Ala

305 310 315 320

Ile Glu Ala Ala Val Ile Arg Pro Pro His Leu Leu Asp Phe Pro Glu

325 330 335

Gln Leu Thr Ile Phe Ser Val Leu Ser Arg Trp Ser Asn Thr Gln Tyr

340 345 350

Met Asn Tyr Trp Val Gly His Arg Leu Glu Ser Arg Thr Ile Arg Gly

355 360 365

Ser Leu Ser Thr Ser Thr His Gly Asn Thr Asn Thr Ser Ile Asn Pro

370 375 380

Val Thr Leu Gln Phe Thr Ser Arg Asp Val Tyr Arg Thr Glu Ser Phe

385 390 395 400

Ala Gly Ile Asn Ile Leu Leu Thr Thr Pro Val Asn Gly Val Pro Trp

405 410 415

Ala Arg Phe Asn Trp Arg Asn Pro Leu Asn Ser Leu Arg Gly Ser Leu

420 425 430

Leu Tyr Thr Ile Gly Tyr Thr Gly Val Gly Thr Gln Leu Phe Asp Ser

435 440 445

Glu Thr Glu Leu Pro Pro Glu Thr Thr Glu Arg Pro Asn Tyr Glu Ser

450 455 460

Tyr Ser His Arg Leu Ser Asn Ile Arg Leu Ile Ser Gly Asn Thr Leu

465 470 475 480

Arg Ala Pro Val Tyr Ser Trp Thr His Arg Ser Ala Asp Arg Thr Asn

485 490 495

Thr Ile Ser Ser Asp Ser Ile Thr Gln Ile Pro Leu Val Lys Ala His

500 505 510

Thr Leu Gln Ser Gly Thr Thr Val Val Lys Gly Pro Gly Phe Thr Gly

515 520 525

Gly Asp Ile Leu Arg Arg Thr Ser Gly Gly Pro Phe Ala Phe Ser Asn

530 535 540

Val Asn Leu Asp Phe Asn Leu Ser Gln Arg Tyr Arg Ala Arg Ile Arg

545 550 555 560

Tyr Ala Ser Thr Thr Asn Leu Arg Ile Tyr Val Thr Val Ala Gly Glu

565 570 575

Arg Ile Phe Ala Gly Gln Phe Asp Lys Thr Met Asp Ala Gly Ala Pro

580 585 590

Leu Thr Phe Gln Ser Phe Ser Tyr Ala Thr Ile Asn Thr Ala Phe Thr

595 600 605

Phe Pro Glu Arg Ser Ser Ser Leu Thr Val Gly Ala Asp Thr Phe Ser

610 615 620

Ser Gly Asn Glu Val Tyr Val Asp Arg Phe Glu Leu Ile Pro Val Thr

625 630 635 640

Ala Thr Asp Ala Thr Phe Glu Ala Glu Ser Asp Leu Glu Arg Ala Gln

645 650 655

Lys Ala Val Asn Ala Leu Phe Thr Ser Ser Asn Gln Ile Gly Leu Lys

660 665 670

Thr Asp Val Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Asp

675 680 685

Cys Leu Ser Asp Glu Phe Cys Leu Asp Glu Lys Arg Glu Leu Ser Glu

690 695 700

Lys Val Lys His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln

705 710 715 720

Asp Pro Asn Phe Arg Gly Ile Asn Arg Gln Pro Asp Arg Gly Trp Arg

725 730 735

Gly Ser Thr Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu

740 745 750

Asn Tyr Val Thr Leu Pro Gly Thr Val Asp Glu Cys Tyr Pro Thr Tyr

755 760 765

Leu Tyr Gln Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr

770 775 780

Glu Leu Arg Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu

785 790 795 800

Ile Arg Tyr Asn Ala Lys His Glu Ile Val Asn Val Pro Gly Thr Gly

805 810 815

Ser Leu Trp Pro Leu Ser Ala Gln Ser Pro Ile Gly Lys Cys Gly Glu

820 825 830

Pro Asn Arg Cys Ala Pro His Leu Glu Trp Asn Pro Asp Leu Asp Cys

835 840 845

Ser Cys Arg Asp Gly Glu Lys Cys Ala His His Ser His His Phe Thr

850 855 860

Leu Asp Ile Asp Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val

865 870 875 880

Trp Val Ile Phe Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly

885 890 895

Asn Leu Glu Phe Leu Glu Glu Lys Pro Leu Leu Gly Glu Ala Leu Ala

900 905 910

Arg Val Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Lys Leu

915 920 925

Gln Leu Glu Thr Asn Ile Val Tyr Lys Glu Ala Lys Glu Ser Val Asp

930 935 940

Ala Leu Phe Val Asn Ser Gln Tyr Asp Arg Leu Gln Val Asp Thr Asn

945 950 955 960

Ile Ala Met Ile His Ala Ala Asp Lys Arg Val His Arg Ile Arg Glu

965 970 975

Ala Tyr Leu Pro Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile

980 985 990

Phe Glu Glu Leu Glu Gly Arg Ile Phe Thr Ala Tyr Ser Leu Tyr Asp

995 1000 1005

Ala Arg Asn Val Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Leu

1010 1015 1020

Cys Trp Asn Val Lys Gly His Val Asp Val Glu Glu Gln Asn Asn

1025 1030 1035

His Arg Ser Val Leu Val Ile Pro Glu Trp Glu Ala Glu Val Ser

1040 1045 1050

Gln Glu Val Arg Val Cys Pro Gly Arg Gly Tyr Ile Leu Arg Val

1055 1060 1065

Thr Ala Tyr Lys Glu Gly Tyr Gly Glu Gly Cys Val Thr Ile His

1070 1075 1080

Glu Ile Glu Asp Asn Thr Asp Glu Leu Lys Phe Ser Asn Cys Val

1085 1090 1095

Glu Glu Glu Val Tyr Pro Asn Asn Thr Val Thr Cys Asn Asn Tyr

1100 1105 1110

Thr Gly Thr Gln Glu Glu Tyr Glu Gly Thr Tyr Thr Ser Arg Asn

1115 1120 1125

Gln Gly Tyr Asp Glu Ala Tyr Gly Asn Asn Pro Ser Val Pro Ala

1130 1135 1140

Asp Tyr Ala Ser Val Tyr Glu Glu Lys Ser Tyr Thr Asp Gly Arg

1145 1150 1155

Arg Glu Asn Pro Cys Glu Ser Asn Arg Gly Tyr Gly Asp Tyr Thr

1160 1165 1170

Pro Leu Pro Ala Gly Tyr Val Thr Lys Asp Leu Glu Tyr Phe Pro

1175 1180 1185

Glu Thr Asp Lys Val Trp Ile Glu Ile Gly Glu Thr Glu Gly Thr

1190 1195 1200

Phe Ile Val Asp Ser Val Glu Leu Leu Leu Met Glu Glu

1205 1210 1215

<210> 26

<211> 3600

<212> DNA

<213> 人工的

<220>

<223> 用于在细菌细胞中表达的编码TIC868的重组核苷酸序列。

<400> 26

atgacttcaa ataggaaaaa tgagaatgaa attataaatg ctttatcgat tccagctgta 60

tcgaatcatt ccgcacaaat gaatctatca accgatgctc gtattgagga tagcttgtgt 120

atagccgagg ggaacaatat cgatccattt gttagcgcat caacagtcca aacgggtatt 180

aacatagctg gtagaatact aggtgtatta ggcgtaccgt ttgctggaca aatagctagt 240

ttttatagtt ttcttgttgg tgaattatgg ccccgcggca gagatccttg ggaaattttc 300

ctagaacatg tcgaacaact tataagacaa caagtaacag aaaatactag ggatacggct 360

cttgctcgat tacaaggttt aggaaattcc tttagagcct atcaacagtc acttgaagat 420

tggctagaaa accgtgatga tgcaagaacg agaagtgttc tttataccca atatatagcc 480

ttagaacttg attttcttaa tgcgatgccg cttttcgcaa ttagaaacca agaagttcca 540

ttattaatgg tatatgctca agctgcaaat ttacacctat tattattgag agatgcctct 600

ctttttggta gtgaatttgg gcttacatcc caagaaattc aacgttatta tgagcgccaa 660

gtggaaaaaa cgagagaata ttctgattat tgcgcaagat ggtataatac gggtttaaat 720

aatttgagag ggacaaatgc tgaaagttgg ttgcgatata atcaattccg tagagactta 780

acgctaggag tattagatct agtggcacta ttcccaagct atgacacgcg tgtttatcca 840

atgaatacca gtgctcaatt aacaagagaa atttatacag atccaattgg gagaacaaat 900

gcaccttcag gatttgcaag tacgaattgg tttaataata atgcaccatc gttttctgcc 960

atagaggctg ccgttattag gcctccgcat ctacttgatt ttccagaaca gcttacaatt 1020

ttcagcgtat taagtcgatg gagtaatact caatatatga attactgggt gggacataga 1080

cttgaatcgc gaacaataag ggggtcatta agtacctcga cacacggaaa taccaatact 1140

tctattaatc ctgtaacatt acagttcaca tctcgagacg tttatagaac agaatcattt 1200

gcagggataa atatacttct aactactcct gtgaatggag taccttgggc tagatttaat 1260

tggagaaatc ccctgaattc tcttagaggt agccttctct atactatagg gtatactgga 1320

gtggggacac aactatttga ttcagaaact gaattaccac cagaaacaac agaacgacca 1380

aattatgaat cttacagtca tagattatct aatataagac taatatcagg aaacactttg 1440

agagcaccag tatattcttg gacgcaccgt agtgcagatc gtacaaatac cattagttca 1500

gatagcatta atcaaatacc tttagtgaaa ggatttagag tttggggggg cacctctgtc 1560

attacaggac caggatttac aggaggggat atccttcgaa gaaatacctt tggtgatttt 1620

gtatctctac aagtcaatat taattcacca attacccaaa gataccgttt aagatttcgt 1680

tacgcttcca gtagggatgc acgagttata gtattaacag gagcggcatc cacaggagtg 1740

ggaggccaag ttagtgtaaa tatgcctctt cagaaaacta tggaaatagg ggagaactta 1800

acatctagaa catttagata taccgatttt agtaatcctt tttcatttag agctaatcca 1860

gatataattg ggataagtga acaacctcta tttggtgcag gttctattag tagcggtgaa 1920

ctttatatag ataaaattga aattattcta gcagatgcaa catttgaagc agaatctgat 1980

ttagaaagag cacaaaaggc ggtgaatgag ctgtttactt cttccaatca aatcgggtta 2040

aaaacagatg tgacggatta tcatattgat caagtatcca atttagttga gtgtttatct 2100

gatgaatttt gtctggatga aaaaaaagaa ttgtccgaga aagtcaaaca tgcgaagcga 2160

cttagtgatg agcggaattt acttcaagat ccaaacttta gagggatcaa tagacaacta 2220

gaccgtggct ggagaggaag tacggatatt accatccaag gaggcgatga cgtattcaaa 2280

gagaattacg ttacgctatt gggtaccttt gatgagtgct atccaacgta tttatatcaa 2340

aaaatagatg agtcgaaatt aaaagcctat acccgttacc aattaagagg gtatatcgaa 2400

gatagtcaag acttagaaat ctatttaatt cgctacaatg ccaaacacga aacagtaaat 2460

gtgccaggta cgggttcctt atggccgctt tcagccccaa gtccaatcgg aaaatgtgcc 2520

catcattccc atcatttctc cttggacatt gatgttggat gtacagactt aaatgaggac 2580

ttaggtgtat gggtgatatt caagattaag acgcaagatg gccatgcaag actaggaaat 2640

ctagaatttc tcgaagagaa accattagta ggagaagcac tagctcgtgt gaaaagagcg 2700

gagaaaaaat ggagagacaa acgtgaaaaa ttggaatggg aaacaaatat tgtttataaa 2760

gaggcaaaag aatctgtaga tgctttattt gtaaactctc aatatgatag attacaagcg 2820

gataccaaca tcgcgatgat tcatgcggca gataaacgcg ttcatagcat tcgagaagct 2880

tatctgcctg agctgtctgt gattccgggt gtcaatgcgg ctatttttga agaattagaa 2940

gggcgtattt tcactgcatt ctccctatat gatgcgagaa atgtcattaa aaatggtgat 3000

tttaataatg gcttatcctg ctggaacgtg aaagggcatg tagatgtaga agaacaaaac 3060

aaccaccgtt cggtccttgt tgttccggaa tgggaagcag aagtgtcaca agaagttcgt 3120

gtctgtccgg gtcgtggcta tatccttcgt gtcacagcgt acaaggaggg atatggagaa 3180

ggttgcgtaa ccattcatga gatcgagaac aatacagacg aactgaagtt tagcaactgt 3240

gtagaagagg aagtatatcc aaacaacacg gtaacgtgta atgattatac tgcgactcaa 3300

gaagaatatg agggtacgta cacttctcgt aatcgaggat atgacggagc ctatgaaagc 3360

aattcttctg taccagctga ttatgcatca gcctatgaag aaaaagcata tacagatgga 3420

cgaagagaca atccttgtga atctaacaga ggatatgggg attacacacc actaccagct 3480

ggctatgtga caaaagaatt agagtacttc ccagaaaccg ataaggtatg gattgagatc 3540

ggagaaacgg aaggaacatt catcgtggac agcgtggaat tacttcttat ggaggaatag 3600

<210> 27

<211> 3600

<212> DNA

<213> 人工的

<220>

<223> 设计用于在植物细胞中表达的编码TIC868的合成核苷酸序列。

<400> 27

atgacgagca accggaagaa cgagaacgag atcatcaacg ccctctcgat ccctgctgtt 60

tcaaaccact ccgcgcagat gaacctgtcc accgacgcgc gcatcgagga ctccctctgc 120

atagccgagg gcaacaacat cgacccattc gtgtcggcca gcacggttca gaccggcatc 180

aacatcgcgg gccgtatcct cggcgtcctc ggtgtcccat tcgccggtca gatcgcgtcc 240

ttctactcgt tccttgtggg cgagctgtgg cctcgcggtc gtgacccgtg ggagatcttc 300

ctggagcatg tggagcagtt gatccggcag caagtcacgg agaacacccg cgatactgct 360

ctggccaggc tacagggcct gggaaactcc tttcgggcat accagcagtc actggaggac 420

tggttggaga acagggatga cgcgcgaaca cgctcggtac tctacaccca gtacatcgct 480

ctcgaactcg acttcctgaa cgctatgccg ctgttcgcca tcaggaacca ggaagttcca 540

ctccttatgg tgtacgccca ggccgccaac ttacatctgc tcctgctgcg ggacgccagc 600

ctgttcggct ccgagttcgg actcacatct caagaaatcc agcgttacta cgagcgccaa 660

gtggagaaga cccgtgagta cagtgactac tgcgctcgat ggtacaacac agggctcaac 720

aacctgcgcg gcaccaacgc tgagtcatgg ctccgttaca accagttccg ccgcgacttg 780

actttgggtg tcctagacct ggtggcgcta ttcccgtctt acgacacacg ggtgtaccca 840

atgaacacta gcgcgcaact cacgcgggag atctacacag acccaatcgg ccggacgaac 900

gcaccctccg gtttcgcatc cacgaattgg ttcaacaaca acgcaccctc cttctcggca 960

atcgaggccg ccgtcatccg ccctcctcac ctgctcgact ttcccgagca gctcacgatc 1020

ttctccgtgc tctcacgctg gtccaacaca cagtacatga actactgggt cgggcaccga 1080

ttggagagta ggacgatccg tggcagcttg agcaccagta cccacggcaa caccaacacc 1140

tccatcaacc cagttacgct acagttcacg agccgcgacg tttaccggac tgagtcgttc 1200

gcgggcatta acatccttct gacaacgccc gtcaacggcg tcccgtgggc ccggttcaac 1260

tggcgtaacc cgttgaactc cctgcgcggg tcattgctct acaccatcgg gtacacgggc 1320

gtcggcaccc agctcttcga cagtgaaact gagctgccgc ccgagaccac ggaacgcccg 1380

aactacgagt cctacagcca ccgcctgtcc aacatccggc tcatctctgg caacacgctg 1440

cgtgcgccgg tgtactcctg gacacaccgc agcgccgacc ggaccaacac gatctcttcc 1500

gactccatta accagatccc gctcgtgaag ggcttccgtg tgtggggtgg cacgagcgtc 1560

atcaccggtc cgggcttcac cggtggagac atactgcggc gcaacacttt cggcgacttc 1620

gtttcgttgc aagtgaacat caactcgccg atcacccagc gttaccgtct gaggttccgc 1680

tacgcttcaa gccgcgacgc gagggtcatt gtcctgaccg gagccgcgtc cacaggcgtg 1740

ggaggccaag tctcagtcaa catgcctctc cagaagacga tggagatagg cgagaacttg 1800

actagccgaa ccttccggta cactgatttc tcgaaccctt tctcattcag agcgaaccct 1860

gacatcattg ggatctccga gcaaccgctg ttcggtgctg gctccatcag ctctggcgaa 1920

ctgtacatcg acaagattga gatcatcctg gcggatgcga cgttcgaggc cgagtctgac 1980

ctggagcggg ctcagaaggc tgtcaacgaa ctgttcacca gcagcaacca gattgggctc 2040

aagaccgacg tcacggacta tcacattgac caagtgtcca accttgtgga gtgcctgtcc 2100

gacgagttct gcctcgacga gaagaaggag ctgtccgaga aggtcaaaca cgcgaagcgt 2160

ctgagtgacg agcggaattt gctccaggac ccgaacttcc gtggcatcaa ccgccagctc 2220

gaccgtggtt ggcgcgggag tacagacatc accatccagg gaggcgacga tgtgttcaag 2280

gagaactatg tgacgctgct cgggactttc gacgaatgct acccgacgta tctctaccag 2340

aagatagacg agagtaaatt gaaggcgtac acccgctacc agcttcgcgg gtacatcgag 2400

gatagtcagg acctggaaat ctacctgatc cgatacaacg ccaagcacga gacagtgaac 2460

gtgccaggca cgggctcact ttggccattg agcgctccct ctccaatcgg aaagtgcgct 2520

caccactcgc accacttctc tctggacatc gacgtgggct gcaccgacct caacgaggac 2580

ctgggtgtct gggttatctt caagattaag acccaggacg gacatgcccg cctcggcaac 2640

ctggagttcc ttgaggagaa gcctctcgtg ggcgaggccc tcgctcgtgt gaagcgcgcc 2700

gagaagaaat ggcgagacaa gcgggagaag ctggagtggg agaccaacat cgtgtacaag 2760

gaggccaagg agtcagtgga cgcactcttc gtcaacagcc agtacgaccg cctccaggct 2820

gacaccaaca tcgccatgat ccacgcggct gacaagcggg tccacagcat ccgtgaggcg 2880

tacctgcccg agctgtcagt gatccctggt gtgaacgcgg cgatcttcga ggaactggag 2940

ggccgcatct tcacagcatt cagcctgtac gatgccagga atgttattaa gaacggtgac 3000

ttcaacaacg ggctgagttg ctggaacgtc aagggccatg tggacgtcga ggagcagaac 3060

aaccaccggt ccgtgctggt cgtgccggag tgggaggcag aggtgagcca ggaggtccgc 3120

gtctgccctg gtcgcggcta catcctccgt gtgactgcgt acaaggaagg ctacggtgaa 3180

ggctgcgtga ctatccacga gatcgagaac aacaccgacg agctcaagtt ctcgaactgt 3240

gtggaggagg aggtgtaccc gaacaacacc gttacttgca acgactacac tgccacgcaa 3300

gaggagtacg agggcactta cacttcccgg aatcgcggct atgatggcgc gtacgagtcc 3360

aacagcagcg tgcctgcgga ttatgcgtcc gcttacgagg agaaggcgta caccgacgga 3420

cggagggaca acccttgcga gtccaaccgt ggctacggtg actacactcc gctgcccgcc 3480

gggtacgtca ccaaggagct ggagtacttc ccggagaccg acaaagtctg gatcgagatc 3540

ggcgagacgg agggcacttt catcgtggac tcggtcgagc tgctactgat ggaggagtga 3600

<210> 28

<211> 1199

<212> PRT

<213> 人工的

<220>

<223> 嵌合蛋白质TIC868的氨基酸序列。

<400> 28

Met Thr Ser Asn Arg Lys Asn Glu Asn Glu Ile Ile Asn Ala Leu Ser

1 5 10 15

Ile Pro Ala Val Ser Asn His Ser Ala Gln Met Asn Leu Ser Thr Asp

20 25 30

Ala Arg Ile Glu Asp Ser Leu Cys Ile Ala Glu Gly Asn Asn Ile Asp

35 40 45

Pro Phe Val Ser Ala Ser Thr Val Gln Thr Gly Ile Asn Ile Ala Gly

50 55 60

Arg Ile Leu Gly Val Leu Gly Val Pro Phe Ala Gly Gln Ile Ala Ser

65 70 75 80

Phe Tyr Ser Phe Leu Val Gly Glu Leu Trp Pro Arg Gly Arg Asp Pro

85 90 95

Trp Glu Ile Phe Leu Glu His Val Glu Gln Leu Ile Arg Gln Gln Val

100 105 110

Thr Glu Asn Thr Arg Asp Thr Ala Leu Ala Arg Leu Gln Gly Leu Gly

115 120 125

Asn Ser Phe Arg Ala Tyr Gln Gln Ser Leu Glu Asp Trp Leu Glu Asn

130 135 140

Arg Asp Asp Ala Arg Thr Arg Ser Val Leu Tyr Thr Gln Tyr Ile Ala

145 150 155 160

Leu Glu Leu Asp Phe Leu Asn Ala Met Pro Leu Phe Ala Ile Arg Asn

165 170 175

Gln Glu Val Pro Leu Leu Met Val Tyr Ala Gln Ala Ala Asn Leu His

180 185 190

Leu Leu Leu Leu Arg Asp Ala Ser Leu Phe Gly Ser Glu Phe Gly Leu

195 200 205

Thr Ser Gln Glu Ile Gln Arg Tyr Tyr Glu Arg Gln Val Glu Lys Thr

210 215 220

Arg Glu Tyr Ser Asp Tyr Cys Ala Arg Trp Tyr Asn Thr Gly Leu Asn

225 230 235 240

Asn Leu Arg Gly Thr Asn Ala Glu Ser Trp Leu Arg Tyr Asn Gln Phe

245 250 255

Arg Arg Asp Leu Thr Leu Gly Val Leu Asp Leu Val Ala Leu Phe Pro

260 265 270

Ser Tyr Asp Thr Arg Val Tyr Pro Met Asn Thr Ser Ala Gln Leu Thr

275 280 285

Arg Glu Ile Tyr Thr Asp Pro Ile Gly Arg Thr Asn Ala Pro Ser Gly

290 295 300

Phe Ala Ser Thr Asn Trp Phe Asn Asn Asn Ala Pro Ser Phe Ser Ala

305 310 315 320

Ile Glu Ala Ala Val Ile Arg Pro Pro His Leu Leu Asp Phe Pro Glu

325 330 335

Gln Leu Thr Ile Phe Ser Val Leu Ser Arg Trp Ser Asn Thr Gln Tyr

340 345 350

Met Asn Tyr Trp Val Gly His Arg Leu Glu Ser Arg Thr Ile Arg Gly

355 360 365

Ser Leu Ser Thr Ser Thr His Gly Asn Thr Asn Thr Ser Ile Asn Pro

370 375 380

Val Thr Leu Gln Phe Thr Ser Arg Asp Val Tyr Arg Thr Glu Ser Phe

385 390 395 400

Ala Gly Ile Asn Ile Leu Leu Thr Thr Pro Val Asn Gly Val Pro Trp

405 410 415

Ala Arg Phe Asn Trp Arg Asn Pro Leu Asn Ser Leu Arg Gly Ser Leu

420 425 430

Leu Tyr Thr Ile Gly Tyr Thr Gly Val Gly Thr Gln Leu Phe Asp Ser

435 440 445

Glu Thr Glu Leu Pro Pro Glu Thr Thr Glu Arg Pro Asn Tyr Glu Ser

450 455 460

Tyr Ser His Arg Leu Ser Asn Ile Arg Leu Ile Ser Gly Asn Thr Leu

465 470 475 480

Arg Ala Pro Val Tyr Ser Trp Thr His Arg Ser Ala Asp Arg Thr Asn

485 490 495

Thr Ile Ser Ser Asp Ser Ile Asn Gln Ile Pro Leu Val Lys Gly Phe

500 505 510

Arg Val Trp Gly Gly Thr Ser Val Ile Thr Gly Pro Gly Phe Thr Gly

515 520 525

Gly Asp Ile Leu Arg Arg Asn Thr Phe Gly Asp Phe Val Ser Leu Gln

530 535 540

Val Asn Ile Asn Ser Pro Ile Thr Gln Arg Tyr Arg Leu Arg Phe Arg

545 550 555 560

Tyr Ala Ser Ser Arg Asp Ala Arg Val Ile Val Leu Thr Gly Ala Ala

565 570 575

Ser Thr Gly Val Gly Gly Gln Val Ser Val Asn Met Pro Leu Gln Lys

580 585 590

Thr Met Glu Ile Gly Glu Asn Leu Thr Ser Arg Thr Phe Arg Tyr Thr

595 600 605

Asp Phe Ser Asn Pro Phe Ser Phe Arg Ala Asn Pro Asp Ile Ile Gly

610 615 620

Ile Ser Glu Gln Pro Leu Phe Gly Ala Gly Ser Ile Ser Ser Gly Glu

625 630 635 640

Leu Tyr Ile Asp Lys Ile Glu Ile Ile Leu Ala Asp Ala Thr Phe Glu

645 650 655

Ala Glu Ser Asp Leu Glu Arg Ala Gln Lys Ala Val Asn Glu Leu Phe

660 665 670

Thr Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp Val Thr Asp Tyr His

675 680 685

Ile Asp Gln Val Ser Asn Leu Val Glu Cys Leu Ser Asp Glu Phe Cys

690 695 700

Leu Asp Glu Lys Lys Glu Leu Ser Glu Lys Val Lys His Ala Lys Arg

705 710 715 720

Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn Phe Arg Gly Ile

725 730 735

Asn Arg Gln Leu Asp Arg Gly Trp Arg Gly Ser Thr Asp Ile Thr Ile

740 745 750

Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val Thr Leu Leu Gly

755 760 765

Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln Lys Ile Asp Glu

770 775 780

Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg Gly Tyr Ile Glu

785 790 795 800

Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr Asn Ala Lys His

805 810 815

Glu Thr Val Asn Val Pro Gly Thr Gly Ser Leu Trp Pro Leu Ser Ala

820 825 830

Pro Ser Pro Ile Gly Lys Cys Ala His His Ser His His Phe Ser Leu

835 840 845

Asp Ile Asp Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp

850 855 860

Val Ile Phe Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn

865 870 875 880

Leu Glu Phe Leu Glu Glu Lys Pro Leu Val Gly Glu Ala Leu Ala Arg

885 890 895

Val Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Lys Leu Glu

900 905 910

Trp Glu Thr Asn Ile Val Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala

915 920 925

Leu Phe Val Asn Ser Gln Tyr Asp Arg Leu Gln Ala Asp Thr Asn Ile

930 935 940

Ala Met Ile His Ala Ala Asp Lys Arg Val His Ser Ile Arg Glu Ala

945 950 955 960

Tyr Leu Pro Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile Phe

965 970 975

Glu Glu Leu Glu Gly Arg Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala

980 985 990

Arg Asn Val Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Ser Cys Trp

995 1000 1005

Asn Val Lys Gly His Val Asp Val Glu Glu Gln Asn Asn His Arg

1010 1015 1020

Ser Val Leu Val Val Pro Glu Trp Glu Ala Glu Val Ser Gln Glu

1025 1030 1035

Val Arg Val Cys Pro Gly Arg Gly Tyr Ile Leu Arg Val Thr Ala

1040 1045 1050

Tyr Lys Glu Gly Tyr Gly Glu Gly Cys Val Thr Ile His Glu Ile

1055 1060 1065

Glu Asn Asn Thr Asp Glu Leu Lys Phe Ser Asn Cys Val Glu Glu

1070 1075 1080

Glu Val Tyr Pro Asn Asn Thr Val Thr Cys Asn Asp Tyr Thr Ala

1085 1090 1095

Thr Gln Glu Glu Tyr Glu Gly Thr Tyr Thr Ser Arg Asn Arg Gly

1100 1105 1110

Tyr Asp Gly Ala Tyr Glu Ser Asn Ser Ser Val Pro Ala Asp Tyr

1115 1120 1125

Ala Ser Ala Tyr Glu Glu Lys Ala Tyr Thr Asp Gly Arg Arg Asp

1130 1135 1140

Asn Pro Cys Glu Ser Asn Arg Gly Tyr Gly Asp Tyr Thr Pro Leu

1145 1150 1155

Pro Ala Gly Tyr Val Thr Lys Glu Leu Glu Tyr Phe Pro Glu Thr

1160 1165 1170

Asp Lys Val Trp Ile Glu Ile Gly Glu Thr Glu Gly Thr Phe Ile

1175 1180 1185

Val Asp Ser Val Glu Leu Leu Leu Met Glu Glu

1190 1195

<210> 29

<211> 3600

<212> DNA

<213> 人工的

<220>

<223> 设计用于在植物细胞中表达的编码TIC868_9的合成核苷酸序列。

<400> 29

atgacgagca accggaagaa cgagaacgag atcatcaacg ccctctcgat ccctgctgtt 60

tcaaaccact ccgcgcagat gaacctgtcc accgacgcgc gcatcgagga ctccctctgc 120

atagccgagg gcaacaacat cgacccattc gtgtcggcca gcacggttca gaccggcatc 180

aacatcgcgg gccgtatcct cggcgtcctc ggtgtcccat tcgccggtca gatcgcgtcc 240

ttctactcgt tccttgtggg cgagctgtgg cctcgcggtc gtgacccgtg ggagatcttc 300

ctggagcatg tggagcagtt gatccggcag caagtcacgg agaacacccg cgatactgct 360

ctggccaggc tacagggcct gggaaactcc tttcgggcat accagcagtc actggaggac 420

tggttggaga acagggatga cgcgcgaaca cgctcggtac tctacaccca gtacatcgct 480

ctcgaactcg acttcctgaa cgctatgccg ctgttcgcca tcaggaacca ggaagttcca 540

ctccttatgg tgtacgccca ggccgccaac ttacatctgc tcctgctgcg ggacgccagc 600

ctgttcggct ccgagttcgg actcacatct caagaaatcc agcgttacta cgagcgccaa 660

gtggagaaga cccgtgagta cagtgactac tgcgctcgat ggtacaacac agggctcaac 720

agcctgcgcg gcaccaacgc tgagtcatgg ctccgttaca accagttccg ccgcgacttg 780

actttgggtg tcctagacct ggtggcgcta ttcccgtctt acgacacacg ggtgtaccca 840

atgaacacta gcgcgcaact cacgcgggag atctacacag acccaatcgg ccggacgaac 900

gcaccctccg gtttcgcatc cacgaattgg ttcaacaaca acgcaccctc cttctcggca 960

atcgaggccg ccgtcatccg ccctcctcac ctgctcgact ttcccgagca gctcacgatc 1020

ttctccgtgc tctcacgctg gtccaacaca cagtacatga actactgggt cgggcaccga 1080

ttggagagta ggacgatccg tggcagcttg agcaccagta cccacggcaa caccaacacc 1140

tccatcaacc cagttacgct acagttcacg agccgcgacg tttaccggac tgagtcgcag 1200

gcgggcatta acatccttat gacaacgccc gtcaacggcg tcccgtgggc ccggttcaac 1260

tggcgtaacc cgaagaactc cctgcgcggg tcattgctct acaccatcgg gtacacgggc 1320

gtcggcaccc agctcttcga cagtgaaact gagctgccgc ccgagaccac ggaacgcccg 1380

aactacgagt cctacagcca ccgcctgtcc aacatccggc tcatctctgg caacacgctg 1440

cgtgcgccgg tgtactcctg gacacaccgc agcgccgacc ggaccaacac gatctcttcc 1500

gactccatta accagatccc gctcgtgaag ggcttccgtg tgtggggtgg cacgagcgtc 1560

atcaccggtc cgggcttcac cggtggagac atactgcggc gcaacacttt cggcgacttc 1620

gtttcgttgc aagtgaacat caactcgccg atcacccagc gttaccgtct gaggttccgc 1680

tacgcttcaa gccgcgacgc gagggtcatt gtcctgaccg gagccgcgtc cacaggcgtg 1740

ggaggccaag tctcagtcaa catgcctctc cagaagacga tggagatagg cgagaacttg 1800

actagccgaa ccttccggta cactgatttc tcgaaccctt tctcattcag agcgaaccct 1860

gacatcattg ggatctccga gcaaccgctg ttcggtgctg gctccatcag ctctggcgaa 1920

ctgtacatcg acaagattga gatcatcctg gcggatgcga cgttcgaggc cgagtctgac 1980

ctggagcggg ctcagaaggc tgtcaacgaa ctgttcacca gcagcaacca gattgggctc 2040

aagaccgacg tcacggacta tcacattgac caagtgtcca accttgtgga gtgcctgtcc 2100

gacgagttct gcctcgacga gaagaaggag ctgtccgaga aggtcaaaca cgcgaagcgt 2160

ctgagtgacg agcggaattt gctccaggac ccgaacttcc gtggcatcaa ccgccagctc 2220

gaccgtggtt ggcgcgggag tacagacatc accatccagg gaggcgacga tgtgttcaag 2280

gagaactatg tgacgctgct cgggactttc gacgaatgct acccgacgta tctctaccag 2340

aagatagacg agagtaaatt gaaggcgtac acccgctacc agcttcgcgg gtacatcgag 2400

gatagtcagg acctggaaat ctacctgatc cgatacaacg ccaagcacga gacagtgaac 2460

gtgccaggca cgggctcact ttggccattg agcgctccct ctccaatcgg aaagtgcgct 2520

caccactcgc accacttctc tctggacatc gacgtgggct gcaccgacct caacgaggac 2580

ctgggtgtct gggttatctt caagattaag acccaggacg gacatgcccg cctcggcaac 2640

ctggagttcc ttgaggagaa gcctctcgtg ggcgaggccc tcgctcgtgt gaagcgcgcc 2700

gagaagaaat ggcgagacaa gcgggagaag ctggagtggg agaccaacat cgtgtacaag 2760

gaggccaagg agtcagtgga cgcactcttc gtcaacagcc agtacgaccg cctccaggct 2820

gacaccaaca tcgccatgat ccacgcggct gacaagcggg tccacagcat ccgtgaggcg 2880

tacctgcccg agctgtcagt gatccctggt gtgaacgcgg cgatcttcga ggaactggag 2940

ggccgcatct tcacagcatt cagcctgtac gatgccagga atgttattaa gaacggtgac 3000

ttcaacaacg ggctgagttg ctggaacgtc aagggccatg tggacgtcga ggagcagaac 3060

aaccaccggt ccgtgctggt cgtgccggag tgggaggcag aggtgagcca ggaggtccgc 3120

gtctgccctg gtcgcggcta catcctccgt gtgactgcgt acaaggaagg ctacggtgaa 3180

ggctgcgtga ctatccacga gatcgagaac aacaccgacg agctcaagtt ctcgaactgt 3240

gtggaggagg aggtgtaccc gaacaacacc gttacttgca acgactacac tgccacgcaa 3300

gaggagtacg agggcactta cacttcccgg aatcgcggct atgatggcgc gtacgagtcc 3360

aacagcagcg tgcctgcgga ttatgcgtcc gcttacgagg agaaggcgta caccgacgga 3420

cggagggaca acccttgcga gtccaaccgt ggctacggtg actacactcc gctgcccgcc 3480

gggtacgtca ccaaggagct ggagtacttc ccggagaccg acaaagtctg gatcgagatc 3540

ggcgagacgg agggcacttt catcgtggac tcggtcgagc tgctactgat ggaggagtga 3600

<210> 30

<211> 1199

<212> PRT

<213> 人工的

<220>

<223> 嵌合蛋白质变体TIC868_9的氨基酸序列。

<400> 30

Met Thr Ser Asn Arg Lys Asn Glu Asn Glu Ile Ile Asn Ala Leu Ser

1 5 10 15

Ile Pro Ala Val Ser Asn His Ser Ala Gln Met Asn Leu Ser Thr Asp

20 25 30

Ala Arg Ile Glu Asp Ser Leu Cys Ile Ala Glu Gly Asn Asn Ile Asp

35 40 45

Pro Phe Val Ser Ala Ser Thr Val Gln Thr Gly Ile Asn Ile Ala Gly

50 55 60

Arg Ile Leu Gly Val Leu Gly Val Pro Phe Ala Gly Gln Ile Ala Ser

65 70 75 80

Phe Tyr Ser Phe Leu Val Gly Glu Leu Trp Pro Arg Gly Arg Asp Pro

85 90 95

Trp Glu Ile Phe Leu Glu His Val Glu Gln Leu Ile Arg Gln Gln Val

100 105 110

Thr Glu Asn Thr Arg Asp Thr Ala Leu Ala Arg Leu Gln Gly Leu Gly

115 120 125

Asn Ser Phe Arg Ala Tyr Gln Gln Ser Leu Glu Asp Trp Leu Glu Asn

130 135 140

Arg Asp Asp Ala Arg Thr Arg Ser Val Leu Tyr Thr Gln Tyr Ile Ala

145 150 155 160

Leu Glu Leu Asp Phe Leu Asn Ala Met Pro Leu Phe Ala Ile Arg Asn

165 170 175

Gln Glu Val Pro Leu Leu Met Val Tyr Ala Gln Ala Ala Asn Leu His

180 185 190

Leu Leu Leu Leu Arg Asp Ala Ser Leu Phe Gly Ser Glu Phe Gly Leu

195 200 205

Thr Ser Gln Glu Ile Gln Arg Tyr Tyr Glu Arg Gln Val Glu Lys Thr

210 215 220

Arg Glu Tyr Ser Asp Tyr Cys Ala Arg Trp Tyr Asn Thr Gly Leu Asn

225 230 235 240

Ser Leu Arg Gly Thr Asn Ala Glu Ser Trp Leu Arg Tyr Asn Gln Phe

245 250 255

Arg Arg Asp Leu Thr Leu Gly Val Leu Asp Leu Val Ala Leu Phe Pro

260 265 270

Ser Tyr Asp Thr Arg Val Tyr Pro Met Asn Thr Ser Ala Gln Leu Thr

275 280 285

Arg Glu Ile Tyr Thr Asp Pro Ile Gly Arg Thr Asn Ala Pro Ser Gly

290 295 300

Phe Ala Ser Thr Asn Trp Phe Asn Asn Asn Ala Pro Ser Phe Ser Ala

305 310 315 320

Ile Glu Ala Ala Val Ile Arg Pro Pro His Leu Leu Asp Phe Pro Glu

325 330 335

Gln Leu Thr Ile Phe Ser Val Leu Ser Arg Trp Ser Asn Thr Gln Tyr

340 345 350

Met Asn Tyr Trp Val Gly His Arg Leu Glu Ser Arg Thr Ile Arg Gly

355 360 365

Ser Leu Ser Thr Ser Thr His Gly Asn Thr Asn Thr Ser Ile Asn Pro

370 375 380

Val Thr Leu Gln Phe Thr Ser Arg Asp Val Tyr Arg Thr Glu Ser Gln

385 390 395 400

Ala Gly Ile Asn Ile Leu Met Thr Thr Pro Val Asn Gly Val Pro Trp

405 410 415

Ala Arg Phe Asn Trp Arg Asn Pro Lys Asn Ser Leu Arg Gly Ser Leu

420 425 430

Leu Tyr Thr Ile Gly Tyr Thr Gly Val Gly Thr Gln Leu Phe Asp Ser

435 440 445

Glu Thr Glu Leu Pro Pro Glu Thr Thr Glu Arg Pro Asn Tyr Glu Ser

450 455 460

Tyr Ser His Arg Leu Ser Asn Ile Arg Leu Ile Ser Gly Asn Thr Leu

465 470 475 480

Arg Ala Pro Val Tyr Ser Trp Thr His Arg Ser Ala Asp Arg Thr Asn

485 490 495

Thr Ile Ser Ser Asp Ser Ile Asn Gln Ile Pro Leu Val Lys Gly Phe

500 505 510

Arg Val Trp Gly Gly Thr Ser Val Ile Thr Gly Pro Gly Phe Thr Gly

515 520 525

Gly Asp Ile Leu Arg Arg Asn Thr Phe Gly Asp Phe Val Ser Leu Gln

530 535 540

Val Asn Ile Asn Ser Pro Ile Thr Gln Arg Tyr Arg Leu Arg Phe Arg

545 550 555 560

Tyr Ala Ser Ser Arg Asp Ala Arg Val Ile Val Leu Thr Gly Ala Ala

565 570 575

Ser Thr Gly Val Gly Gly Gln Val Ser Val Asn Met Pro Leu Gln Lys

580 585 590

Thr Met Glu Ile Gly Glu Asn Leu Thr Ser Arg Thr Phe Arg Tyr Thr

595 600 605

Asp Phe Ser Asn Pro Phe Ser Phe Arg Ala Asn Pro Asp Ile Ile Gly

610 615 620

Ile Ser Glu Gln Pro Leu Phe Gly Ala Gly Ser Ile Ser Ser Gly Glu

625 630 635 640

Leu Tyr Ile Asp Lys Ile Glu Ile Ile Leu Ala Asp Ala Thr Phe Glu

645 650 655

Ala Glu Ser Asp Leu Glu Arg Ala Gln Lys Ala Val Asn Glu Leu Phe

660 665 670

Thr Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp Val Thr Asp Tyr His

675 680 685

Ile Asp Gln Val Ser Asn Leu Val Glu Cys Leu Ser Asp Glu Phe Cys

690 695 700

Leu Asp Glu Lys Lys Glu Leu Ser Glu Lys Val Lys His Ala Lys Arg

705 710 715 720

Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn Phe Arg Gly Ile

725 730 735

Asn Arg Gln Leu Asp Arg Gly Trp Arg Gly Ser Thr Asp Ile Thr Ile

740 745 750

Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val Thr Leu Leu Gly

755 760 765

Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln Lys Ile Asp Glu

770 775 780

Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg Gly Tyr Ile Glu

785 790 795 800

Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr Asn Ala Lys His

805 810 815

Glu Thr Val Asn Val Pro Gly Thr Gly Ser Leu Trp Pro Leu Ser Ala

820 825 830

Pro Ser Pro Ile Gly Lys Cys Ala His His Ser His His Phe Ser Leu

835 840 845

Asp Ile Asp Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp

850 855 860

Val Ile Phe Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn

865 870 875 880

Leu Glu Phe Leu Glu Glu Lys Pro Leu Val Gly Glu Ala Leu Ala Arg

885 890 895

Val Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Lys Leu Glu

900 905 910

Trp Glu Thr Asn Ile Val Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala

915 920 925

Leu Phe Val Asn Ser Gln Tyr Asp Arg Leu Gln Ala Asp Thr Asn Ile

930 935 940

Ala Met Ile His Ala Ala Asp Lys Arg Val His Ser Ile Arg Glu Ala

945 950 955 960

Tyr Leu Pro Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile Phe

965 970 975

Glu Glu Leu Glu Gly Arg Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala

980 985 990

Arg Asn Val Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Ser Cys Trp

995 1000 1005

Asn Val Lys Gly His Val Asp Val Glu Glu Gln Asn Asn His Arg

1010 1015 1020

Ser Val Leu Val Val Pro Glu Trp Glu Ala Glu Val Ser Gln Glu

1025 1030 1035

Val Arg Val Cys Pro Gly Arg Gly Tyr Ile Leu Arg Val Thr Ala

1040 1045 1050

Tyr Lys Glu Gly Tyr Gly Glu Gly Cys Val Thr Ile His Glu Ile

1055 1060 1065

Glu Asn Asn Thr Asp Glu Leu Lys Phe Ser Asn Cys Val Glu Glu

1070 1075 1080

Glu Val Tyr Pro Asn Asn Thr Val Thr Cys Asn Asp Tyr Thr Ala

1085 1090 1095

Thr Gln Glu Glu Tyr Glu Gly Thr Tyr Thr Ser Arg Asn Arg Gly

1100 1105 1110

Tyr Asp Gly Ala Tyr Glu Ser Asn Ser Ser Val Pro Ala Asp Tyr

1115 1120 1125

Ala Ser Ala Tyr Glu Glu Lys Ala Tyr Thr Asp Gly Arg Arg Asp

1130 1135 1140

Asn Pro Cys Glu Ser Asn Arg Gly Tyr Gly Asp Tyr Thr Pro Leu

1145 1150 1155

Pro Ala Gly Tyr Val Thr Lys Glu Leu Glu Tyr Phe Pro Glu Thr

1160 1165 1170

Asp Lys Val Trp Ile Glu Ile Gly Glu Thr Glu Gly Thr Phe Ile

1175 1180 1185

Val Asp Ser Val Glu Leu Leu Leu Met Glu Glu

1190 1195

<210> 31

<211> 3678

<212> DNA

<213> 人工的

<220>

<223> 用于在细菌细胞中表达的编码TIC868_10的重组核苷酸序列。

<400> 31

atgacttcaa ataggaaaaa tgagaatgaa attataaatg ctttatcgat tccagctgta 60

tcgaatcatt ccgcacaaat gaatctatca accgatgctc gtattgagga tagcttgtgt 120

atagccgagg ggaacaatat cgatccattt gttagcgcat caacagtcca aacgggtatt 180

aacatagctg gtagaatact aggtgtatta ggcgtaccgt ttgctggaca aatagctagt 240

ttttatagtt ttcttgttgg tgaattatgg ccccgcggca gagatccttg ggaaattttc 300

ctagaacatg tcgaacaact tataagacaa caagtaacag aaaatactag ggatacggct 360

cttgctcgat tacaaggttt aggaaattcc tttagagcct atcaacagtc acttgaagat 420

tggctagaaa accgtgatga tgcaagaacg agaagtgttc tttataccca atatatagcc 480

ttagaacttg attttcttaa tgcgatgccg cttttcgcaa ttagaaacca agaagttcca 540

ttattaatgg tatatgctca agctgcaaat ttacacctat tattattgag agatgcctct 600

ctttttggta gtgaatttgg gcttacatcc caagaaattc aacgttatta tgagcgccaa 660

gtggaaaaaa cgagagaata ttctgattat tgcgcaagat ggtataatac gggtttaaat 720

aatttgagag ggacaaatgc tgaaagttgg ttgcgatata atcaattccg tagagactta 780

acgctaggag tattagatct agtggcacta ttcccaagct atgacacgcg tgtttatcca 840

atgaatacca gtgctcaatt aacaagagaa atttatacag atccaattgg gagaacaaat 900

gcaccttcag gatttgcaag tacgaattgg tttaataata atgcaccatc gttttctgcc 960

atagaggctg ccgttattag gcctccgcat ctacttgatt ttccagaaca gcttacaatt 1020

ttcagcgtat taagtcgatg gagtaatact caatatatga attactgggt gggacataga 1080

cttgaatcgc gaacaataag ggggtcatta agtacctcga cacacggaaa taccaatact 1140

tctattaatc ctgtaacatt acagttcaca tctcgagacg tttatagaac agaatcattt 1200

gcagggataa atatacttct aactactcct gtgaatggag taccttgggc tagatttaat 1260

tggagaaatc ccctgaattc tcttagaggt agccttctct atactatagg gtatactgga 1320

gtggggacac aactatttga ttcagaaact gaattaccac cagaaacaac agaacgacca 1380

aattatgaat cttacagtca tagattatct aatataagac taatatcagg aaacactttg 1440

agagcaccag tatattcttg gacgcaccgt agtgcagatc gtacaaatac cattagttca 1500

gatagcatta atcaaatacc tttagtgaaa ggatttagag tttggggggg cacctctgtc 1560

attacaggac caggatttac aggaggggat atccttcgaa gaaatacctt tggtgatttt 1620

gtatctctac aagtcaatat taattcacca attacccaaa gataccgttt aagatttcgt 1680

tacgcttcca gtagggatgc acgagttata gtattaacag gagcggcatc cacaggagtg 1740

ggaggccaag ttagtgtaaa tatgcctctt cagaaaacta tggaaatagg ggagaactta 1800

acatctagaa catttagata taccgatttt agtaatcctt tttcatttag agctaatcca 1860

gatataattg ggataagtga acaacctcta tttggtgcag gttctattag tagcggtgaa 1920

ctttatatag ataaaattga aattattcta gcagatgcaa catttgaggc agaatatgat 1980

ttagaaagag cgcaaaaggt ggtgaatgcc ctgtttacgt ctacaaacca actagggcta 2040

aaaacagatg tgacggatta tcatattgat caggtatcca atctagttgc gtgtttatcg 2100

gatgaatttt gtctggatga aaagagagaa ttgtccgaga aagttaaaca tgcaaagcga 2160

ctcagtgatg agcggaattt acttcaagat ccaaacttca gagggatcaa taggcaacca 2220

gaccgtggct ggagaggaag tacggatatt actatccaag gaggagatga cgtattcaaa 2280

gagaattacg ttacgctacc gggtaccttt gatgagtgct atccaacgta tttatatcaa 2340

aaaatagatg agtcgaaatt aaaagcctat acccgttatc aattaagagg gtatatcgaa 2400

gatagtcaag acttagaaat ctatttaatt cgttacaatg caaaacacga aatagtaaat 2460

gtaccaggta caggaagttt atggcctctt tctgtagaaa atcaaattgg accttgtgga 2520

gaaccgaatc gatgcgcgcc acaccttgaa tggaatcctg atttacactg ttcctgcaga 2580

gacggggaaa aatgtgcaca tcattctcat catttctctt tggacattga tgttggatgt 2640

acagacttaa atgaggactt aggtgtatgg gtgatattca agattaagac gcaagatggc 2700

cacgcacgac tagggaatct agagtttctc gaagagaaac cattattagg agaagcacta 2760

gctcgtgtga aaagagcgga gaaaaaatgg agagacaaac gcgaaacatt acaattggaa 2820

acaactatcg tttataaaga ggcaaaagaa tctgtagatg ctttatttgt aaactctcaa 2880

tatgatagat tacaagcgga tacgaacatc gcgatgattc atgcggcaga taaacgcgtt 2940

catagaattc gagaagcgta tctgccggag ctgtctgtga ttccgggtgt caatgcggct 3000

atttttgaag aattagaaga gcgtattttc actgcatttt ccctatatga tgcgagaaat 3060

attattaaaa atggcgattt caataatggc ttattatgct ggaacgtgaa agggcatgta 3120

gaggtagaag aacaaaacaa tcaccgttca gtcctggtta tcccagaatg ggaggcagaa 3180

gtgtcacaag aggttcgtgt ctgtccaggt cgtggctata tccttcgtgt tacagcgtac 3240

aaagagggat atggagaagg ttgcgtaacg atccatgaga tcgagaacaa tacagacgaa 3300

ctgaaattca acaactgtgt agaagaggaa gtatatccaa acaacacggt aacgtgtatt 3360

aattatactg cgactcaaga agaatatgag ggtacgtaca cttctcgtaa tcgaggatat 3420

gacgaagcct atggtaataa cccttccgta ccagctgatt atgcgtcagt ctatgaagaa 3480

aaatcgtata cagatagacg aagagagaat ccttgtgaat ctaacagagg atatggagat 3540

tacacaccac taccagctgg ttatgtaaca aaggaattag agtacttccc agagaccgat 3600

aaggtatgga ttgagattgg agaaacagaa ggaacattca tcgtggacag cgtggaatta 3660

ctccttatgg aggaatag 3678

<210> 32

<211> 3678

<212> DNA

<213> 人工的

<220>

<223> 设计用于在植物细胞中表达的编码TIC868_10的合成核苷酸序列。

<400> 32

atgacgagca accggaagaa cgagaacgag atcatcaacg ccctctcgat ccctgctgtt 60

tcaaaccact ccgcgcagat gaacctgtcc accgacgcgc gcatcgagga ctccctctgc 120

atagccgagg gcaacaacat cgacccattc gtgtcggcca gcacggttca gaccggcatc 180

aacatcgcgg gccgtatcct cggcgtcctc ggtgtcccat tcgccggtca gatcgcgtcc 240

ttctactcgt tccttgtggg cgagctgtgg cctcgcggtc gtgacccgtg ggagatcttc 300

ctggagcatg tggagcagtt gatccggcag caagtcacgg agaacacccg cgatactgct 360

ctggccaggc tacagggcct gggaaactcc tttcgggcat accagcagtc actggaggac 420

tggttggaga acagggatga cgcgcgaaca cgctcggtac tctacaccca gtacatcgct 480

ctcgaactcg acttcctgaa cgctatgccg ctgttcgcca tcaggaacca ggaagttcca 540

ctccttatgg tgtacgccca ggccgccaac ttacatctgc tcctgctgcg ggacgccagc 600

ctgttcggct ccgagttcgg actcacatct caagaaatcc agcgttacta cgagcgccaa 660

gtggagaaga cccgtgagta cagtgactac tgcgctcgat ggtacaacac agggctcaac 720

aacctgcgcg gcaccaacgc tgagtcatgg ctccgttaca accagttccg ccgcgacttg 780

actttgggtg tcctagacct ggtggcgcta ttcccgtctt acgacacacg ggtgtaccca 840

atgaacacta gcgcgcaact cacgcgggag atctacacag acccaatcgg ccggacgaac 900

gcaccctccg gtttcgcatc cacgaattgg ttcaacaaca acgcaccctc cttctcggca 960

atcgaggccg ccgtcatccg ccctcctcac ctgctcgact ttcccgagca gctcacgatc 1020

ttctccgtgc tctcacgctg gtccaacaca cagtacatga actactgggt cgggcaccga 1080

ttggagagta ggacgatccg tggcagcttg agcaccagta cccacggcaa caccaacacc 1140

tccatcaacc cagttacgct acagttcacg agccgcgacg tttaccggac tgagtcgttc 1200

gcgggcatta acatccttct gacaacgccc gtcaacggcg tcccgtgggc ccggttcaac 1260

tggcgtaacc cgttgaactc cctgcgcggg tcattgctct acaccatcgg gtacacgggc 1320

gtcggcaccc agctcttcga cagtgaaact gagctgccgc ccgagaccac ggaacgcccg 1380

aactacgagt cctacagcca ccgcctgtcc aacatccggc tcatctctgg caacacgctg 1440

cgtgcgccgg tgtactcctg gacacaccgc agcgccgacc ggaccaacac gatctcttcc 1500

gactccatta accagatccc gctcgtgaag ggcttccgtg tgtggggtgg cacgagcgtc 1560

atcaccggtc cgggcttcac cggtggagac atactgcggc gcaacacttt cggcgacttc 1620

gtttcgttgc aagtgaacat caactcgccg atcacccagc gttaccgtct gaggttccgc 1680

tacgcttcaa gccgcgacgc gagggtcatt gtcctgaccg gagccgcgtc cacaggcgtg 1740

ggaggccaag tctcagtcaa catgcctctc cagaagacga tggagatagg cgagaacttg 1800

actagccgaa ccttccggta cactgatttc tcgaaccctt tctcattcag agcgaaccct 1860

gacatcattg ggatctccga gcaaccgctg ttcggtgctg gctccatcag ctctggcgaa 1920

ctgtacatcg acaagattga gatcatcctg gcggatgcga cgttcgaggc cgagtacgac 1980

cttgagcgcg cccagaaggt ggtgaacgcc ctcttcacta gcactaacca gctaggcctg 2040

aagactgacg tgaccgacta ccacatcgac caagtgagca acctagtggc ctgcctctcc 2100

gacgagttct gcctcgacga gaagcgcgag ctgtccgaga aggtgaagca cgccaagcgc 2160

ctctccgacg agcgcaacct gctccaggac cccaacttca ggggcatcaa caggcagccc 2220

gaccgcggct ggcgcggctc caccgacatc accatccagg gcggtgacga cgtattcaag 2280

gagaactacg ttaccctccc cggcaccttc gacgagtgtt accccaccta cctctaccag 2340

aagatcgacg agtccaagct gaaggcctac acccgctacc agctccgcgg ctacatcgag 2400

gactcccagg acctggaaat ctacctcatc cgctacaacg ccaagcacga gatcgtgaac 2460

gtgcctggca ccggcagcct ctggcctctc agcgtggaga accagatcgg cccttgcggc 2520

gagcctaacc gctgcgcccc tcacctcgag tggaaccctg acctccactg ctcgtgcagg 2580

gacggcgaga agtgcgccca ccatagccac cacttctctc tggacatcga cgtgggctgc 2640

accgacctga acgaggacct gggcgtgtgg gttatcttca agatcaagac ccaggacggt 2700

cacgccaggc tgggtaacct ggagttcctt gaggaaaagc ctctgctggg tgaggccctg 2760

gccagggtca agagggctga gaagaaatgg agggataaga gggagaccct gcagctggag 2820

accactatcg tctacaagga ggctaaggag tctgtcgatg ctctgttcgt caactctcag 2880

tacgatagac tgcaagctga taccaacatc gctatgatcc acgctgcgga taagcgggtc 2940

caccggatcc gggaggctta ccttccggag ctttctgtca tcccgggtgt caacgctgcg 3000

atcttcgagg aacttgagga acggatcttc actgcgttta gtctttacga tgcgcggaac 3060

atcatcaaga acggggactt caacaatggt ctgctgtgct ggaacgtcaa gggtcatgtc 3120

gaggtcgagg aacaaaacaa tcatcgtagt gtccttgtca ttcctgagtg ggaggcggag 3180

gtctctcaag aggtccgtgt ttgcccgggg cgtgggtaca ttcttcgtgt tactgcgtac 3240

aaggaggggt acggggaggg gtgcgttact attcatgaga ttgagaacaa tactgatgag 3300

cttaagttca acaattgtgt tgaggaggag gtttacccga acaatactgt tacgtgcatc 3360

aactacacgg caacgcaaga ggaatacgag gggacgtaca cctcgcgtaa tagagggtat 3420

gatgaggcgt acggaaacaa cccgtcggtt ccagcagatt atgcctcggt ttatgaggag 3480

aagtcgtaca cggatagacg acgcgagaat ccatgtgagt caaatcgagg atacggagat 3540

tacacaccat taccagcagg atacgttaca aaggagttgg aatacttccc ggaaacagat 3600

aaagtttgga ttgaaatcgg agaaacagaa ggaacattca tcgtcgactc agtagaattg 3660

ttgttgatgg aagaatga 3678

<210> 33

<211> 1225

<212> PRT

<213> 人工的

<220>

<223> 嵌合蛋白质变体TIC868_10的氨基酸序列。

<400> 33

Met Thr Ser Asn Arg Lys Asn Glu Asn Glu Ile Ile Asn Ala Leu Ser

1 5 10 15

Ile Pro Ala Val Ser Asn His Ser Ala Gln Met Asn Leu Ser Thr Asp

20 25 30

Ala Arg Ile Glu Asp Ser Leu Cys Ile Ala Glu Gly Asn Asn Ile Asp

35 40 45

Pro Phe Val Ser Ala Ser Thr Val Gln Thr Gly Ile Asn Ile Ala Gly

50 55 60

Arg Ile Leu Gly Val Leu Gly Val Pro Phe Ala Gly Gln Ile Ala Ser

65 70 75 80

Phe Tyr Ser Phe Leu Val Gly Glu Leu Trp Pro Arg Gly Arg Asp Pro

85 90 95

Trp Glu Ile Phe Leu Glu His Val Glu Gln Leu Ile Arg Gln Gln Val

100 105 110

Thr Glu Asn Thr Arg Asp Thr Ala Leu Ala Arg Leu Gln Gly Leu Gly

115 120 125

Asn Ser Phe Arg Ala Tyr Gln Gln Ser Leu Glu Asp Trp Leu Glu Asn

130 135 140

Arg Asp Asp Ala Arg Thr Arg Ser Val Leu Tyr Thr Gln Tyr Ile Ala

145 150 155 160

Leu Glu Leu Asp Phe Leu Asn Ala Met Pro Leu Phe Ala Ile Arg Asn

165 170 175

Gln Glu Val Pro Leu Leu Met Val Tyr Ala Gln Ala Ala Asn Leu His

180 185 190

Leu Leu Leu Leu Arg Asp Ala Ser Leu Phe Gly Ser Glu Phe Gly Leu

195 200 205

Thr Ser Gln Glu Ile Gln Arg Tyr Tyr Glu Arg Gln Val Glu Lys Thr

210 215 220

Arg Glu Tyr Ser Asp Tyr Cys Ala Arg Trp Tyr Asn Thr Gly Leu Asn

225 230 235 240

Asn Leu Arg Gly Thr Asn Ala Glu Ser Trp Leu Arg Tyr Asn Gln Phe

245 250 255

Arg Arg Asp Leu Thr Leu Gly Val Leu Asp Leu Val Ala Leu Phe Pro

260 265 270

Ser Tyr Asp Thr Arg Val Tyr Pro Met Asn Thr Ser Ala Gln Leu Thr

275 280 285

Arg Glu Ile Tyr Thr Asp Pro Ile Gly Arg Thr Asn Ala Pro Ser Gly

290 295 300

Phe Ala Ser Thr Asn Trp Phe Asn Asn Asn Ala Pro Ser Phe Ser Ala

305 310 315 320

Ile Glu Ala Ala Val Ile Arg Pro Pro His Leu Leu Asp Phe Pro Glu

325 330 335

Gln Leu Thr Ile Phe Ser Val Leu Ser Arg Trp Ser Asn Thr Gln Tyr

340 345 350

Met Asn Tyr Trp Val Gly His Arg Leu Glu Ser Arg Thr Ile Arg Gly

355 360 365

Ser Leu Ser Thr Ser Thr His Gly Asn Thr Asn Thr Ser Ile Asn Pro

370 375 380

Val Thr Leu Gln Phe Thr Ser Arg Asp Val Tyr Arg Thr Glu Ser Phe

385 390 395 400

Ala Gly Ile Asn Ile Leu Leu Thr Thr Pro Val Asn Gly Val Pro Trp

405 410 415

Ala Arg Phe Asn Trp Arg Asn Pro Leu Asn Ser Leu Arg Gly Ser Leu

420 425 430

Leu Tyr Thr Ile Gly Tyr Thr Gly Val Gly Thr Gln Leu Phe Asp Ser

435 440 445

Glu Thr Glu Leu Pro Pro Glu Thr Thr Glu Arg Pro Asn Tyr Glu Ser

450 455 460

Tyr Ser His Arg Leu Ser Asn Ile Arg Leu Ile Ser Gly Asn Thr Leu

465 470 475 480

Arg Ala Pro Val Tyr Ser Trp Thr His Arg Ser Ala Asp Arg Thr Asn

485 490 495

Thr Ile Ser Ser Asp Ser Ile Asn Gln Ile Pro Leu Val Lys Gly Phe

500 505 510

Arg Val Trp Gly Gly Thr Ser Val Ile Thr Gly Pro Gly Phe Thr Gly

515 520 525

Gly Asp Ile Leu Arg Arg Asn Thr Phe Gly Asp Phe Val Ser Leu Gln

530 535 540

Val Asn Ile Asn Ser Pro Ile Thr Gln Arg Tyr Arg Leu Arg Phe Arg

545 550 555 560

Tyr Ala Ser Ser Arg Asp Ala Arg Val Ile Val Leu Thr Gly Ala Ala

565 570 575

Ser Thr Gly Val Gly Gly Gln Val Ser Val Asn Met Pro Leu Gln Lys

580 585 590

Thr Met Glu Ile Gly Glu Asn Leu Thr Ser Arg Thr Phe Arg Tyr Thr

595 600 605

Asp Phe Ser Asn Pro Phe Ser Phe Arg Ala Asn Pro Asp Ile Ile Gly

610 615 620

Ile Ser Glu Gln Pro Leu Phe Gly Ala Gly Ser Ile Ser Ser Gly Glu

625 630 635 640

Leu Tyr Ile Asp Lys Ile Glu Ile Ile Leu Ala Asp Ala Thr Phe Glu

645 650 655

Ala Glu Tyr Asp Leu Glu Arg Ala Gln Lys Val Val Asn Ala Leu Phe

660 665 670

Thr Ser Thr Asn Gln Leu Gly Leu Lys Thr Asp Val Thr Asp Tyr His

675 680 685

Ile Asp Gln Val Ser Asn Leu Val Ala Cys Leu Ser Asp Glu Phe Cys

690 695 700

Leu Asp Glu Lys Arg Glu Leu Ser Glu Lys Val Lys His Ala Lys Arg

705 710 715 720

Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn Phe Arg Gly Ile

725 730 735

Asn Arg Gln Pro Asp Arg Gly Trp Arg Gly Ser Thr Asp Ile Thr Ile

740 745 750

Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val Thr Leu Pro Gly

755 760 765

Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln Lys Ile Asp Glu

770 775 780

Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg Gly Tyr Ile Glu

785 790 795 800

Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr Asn Ala Lys His

805 810 815

Glu Ile Val Asn Val Pro Gly Thr Gly Ser Leu Trp Pro Leu Ser Val

820 825 830

Glu Asn Gln Ile Gly Pro Cys Gly Glu Pro Asn Arg Cys Ala Pro His

835 840 845

Leu Glu Trp Asn Pro Asp Leu His Cys Ser Cys Arg Asp Gly Glu Lys

850 855 860

Cys Ala His His Ser His His Phe Ser Leu Asp Ile Asp Val Gly Cys

865 870 875 880

Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Val Ile Phe Lys Ile Lys

885 890 895

Thr Gln Asp Gly His Ala Arg Leu Gly Asn Leu Glu Phe Leu Glu Glu

900 905 910

Lys Pro Leu Leu Gly Glu Ala Leu Ala Arg Val Lys Arg Ala Glu Lys

915 920 925

Lys Trp Arg Asp Lys Arg Glu Thr Leu Gln Leu Glu Thr Thr Ile Val

930 935 940

Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala Leu Phe Val Asn Ser Gln

945 950 955 960

Tyr Asp Arg Leu Gln Ala Asp Thr Asn Ile Ala Met Ile His Ala Ala

965 970 975

Asp Lys Arg Val His Arg Ile Arg Glu Ala Tyr Leu Pro Glu Leu Ser

980 985 990

Val Ile Pro Gly Val Asn Ala Ala Ile Phe Glu Glu Leu Glu Glu Arg

995 1000 1005

Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala Arg Asn Ile Ile Lys

1010 1015 1020

Asn Gly Asp Phe Asn Asn Gly Leu Leu Cys Trp Asn Val Lys Gly

1025 1030 1035

His Val Glu Val Glu Glu Gln Asn Asn His Arg Ser Val Leu Val

1040 1045 1050

Ile Pro Glu Trp Glu Ala Glu Val Ser Gln Glu Val Arg Val Cys

1055 1060 1065

Pro Gly Arg Gly Tyr Ile Leu Arg Val Thr Ala Tyr Lys Glu Gly

1070 1075 1080

Tyr Gly Glu Gly Cys Val Thr Ile His Glu Ile Glu Asn Asn Thr

1085 1090 1095

Asp Glu Leu Lys Phe Asn Asn Cys Val Glu Glu Glu Val Tyr Pro

1100 1105 1110

Asn Asn Thr Val Thr Cys Ile Asn Tyr Thr Ala Thr Gln Glu Glu

1115 1120 1125

Tyr Glu Gly Thr Tyr Thr Ser Arg Asn Arg Gly Tyr Asp Glu Ala

1130 1135 1140

Tyr Gly Asn Asn Pro Ser Val Pro Ala Asp Tyr Ala Ser Val Tyr

1145 1150 1155

Glu Glu Lys Ser Tyr Thr Asp Arg Arg Arg Glu Asn Pro Cys Glu

1160 1165 1170

Ser Asn Arg Gly Tyr Gly Asp Tyr Thr Pro Leu Pro Ala Gly Tyr

1175 1180 1185

Val Thr Lys Glu Leu Glu Tyr Phe Pro Glu Thr Asp Lys Val Trp

1190 1195 1200

Ile Glu Ile Gly Glu Thr Glu Gly Thr Phe Ile Val Asp Ser Val

1205 1210 1215

Glu Leu Leu Leu Met Glu Glu

1220 1225

<210> 34

<211> 3726

<212> DNA

<213> 人工的

<220>

<223> 用于在细菌细胞中表达的编码TIC868_11的重组核苷酸序列。

<400> 34

atgacttcaa ataggaaaaa tgagaatgaa attataaatg ctttatcgat tccagctgta 60

tcgaatcatt ccgcacaaat gaatctatca accgatgctc gtattgagga tagcttgtgt 120

atagccgagg ggaacaatat cgatccattt gttagcgcat caacagtcca aacgggtatt 180

aacatagctg gtagaatact aggtgtatta ggcgtaccgt ttgctggaca aatagctagt 240

ttttatagtt ttcttgttgg tgaattatgg ccccgcggca gagatccttg ggaaattttc 300

ctagaacatg tcgaacaact tataagacaa caagtaacag aaaatactag ggatacggct 360

cttgctcgat tacaaggttt aggaaattcc tttagagcct atcaacagtc acttgaagat 420

tggctagaaa accgtgatga tgcaagaacg agaagtgttc tttataccca atatatagcc 480

ttagaacttg attttcttaa tgcgatgccg cttttcgcaa ttagaaacca agaagttcca 540

ttattaatgg tatatgctca agctgcaaat ttacacctat tattattgag agatgcctct 600

ctttttggta gtgaatttgg gcttacatcc caagaaattc aacgttatta tgagcgccaa 660

gtggaaaaaa cgagagaata ttctgattat tgcgcaagat ggtataatac gggtttaaat 720

aatttgagag ggacaaatgc tgaaagttgg ttgcgatata atcaattccg tagagactta 780

acgctaggag tattagatct agtggcacta ttcccaagct atgacacgcg tgtttatcca 840

atgaatacca gtgctcaatt aacaagagaa atttatacag atccaattgg gagaacaaat 900

gcaccttcag gatttgcaag tacgaattgg tttaataata atgcaccatc gttttctgcc 960

atagaggctg ccgttattag gcctccgcat ctacttgatt ttccagaaca gcttacaatt 1020

ttcagcgtat taagtcgatg gagtaatact caatatatga attactgggt gggacataga 1080

cttgaatcgc gaacaataag ggggtcatta agtacctcga cacacggaaa taccaatact 1140

tctattaatc ctgtaacatt acagttcaca tctcgagacg tttatagaac agaatcattt 1200

gcagggataa atatacttct aactactcct gtgaatggag taccttgggc tagatttaat 1260

tggagaaatc ccctgaattc tcttagaggt agccttctct atactatagg gtatactgga 1320

gtggggacac aactatttga ttcagaaact gaattaccac cagaaacaac agaacgacca 1380

aattatgaat cttacagtca tagattatct aatataagac taatatcagg aaacactttg 1440

agagcaccag tatattcttg gacgcaccgt agtgcagatc gtacaaatac cattagttca 1500

gatagcatta atcaaatacc tttagtgaaa ggatttagag tttggggggg cacctctgtc 1560

attacaggac caggatttac aggaggggat atccttcgaa gaaatacctt tggtgatttt 1620

gtatctctac aagtcaatat taattcacca attacccaaa gataccgttt aagatttcgt 1680

tacgcttcca gtagggatgc acgagttata gtattaacag gagcggcatc cacaggagtg 1740

ggaggccaag ttagtgtaaa tatgcctctt cagaaaacta tggaaatagg ggagaactta 1800

acatctagaa catttagata taccgatttt agtaatcctt tttcatttag agctaatcca 1860

gatataattg ggataagtga acaacctcta tttggtgcag gttctattag tagcggtgaa 1920

ctttatatag ataaaattga aattattcta gcagatgcaa caggaacgac aacctatgag 1980

tatgaagaga agcagaatct agaaaaagcg cagaaagcgt tgaacgcttt gtttacggat 2040

ggcacgaatg gctatctaca aatggatgcc actgattatg atatcaatca aactgcaaac 2100

ttaatagaat gtgtatcaga tgaattgtat gcaaaagaaa agatagtttt attagatgaa 2160

gtcaaatatg cgaagcggct tagcatatca cgtaacctac ttttgaacga tgatttagaa 2220

ttttcagatg gatttggaga aaacggatgg acgacaagtg ataatatttc aatccaggcg 2280

gataatcccc tttttaaggg gaattattta aaaatgtttg gggcaagaga tattgatgga 2340

accctatttc caacttatct ctatcaaaaa atagatgagt ccaggttaaa accatataca 2400

cgttatcgag taagagggtt tgtgggaagt agtaaaaatc taaaattagt ggtaacacgc 2460

tatgagaaag aaattgatgc cattatgaat gttccaaatg atttggcaca tatgcagctt 2520

aacccttcat gtggagatta tcgctgtgaa tcatcgtccc agtttttggt gaaccaagtg 2580

catcctacac caacagctgg atatgctctt gatatgtatg catgcccgtc aagttcagat 2640

aaaaaacata ttatgtgtca cgatcgtcat ccatttgatt ttcatattga caccggagaa 2700

ttaaatccaa acacaaacct gggtattgat gtcttgttta aaatttctaa tccaaatgga 2760

tacgctacat tagggaatct agaagtcatt gaagaaggac cactaacaga tgaagcattg 2820

gtacatgtaa aacaaaagga aaagaaatgg cgtcagcaca tggagaaaaa acgaatggaa 2880

acacaacaag cctatgatcc agcaaaacaa gctgtagatg cattatttac aaatgaacaa 2940

gagttagact atcatactac tttagatcat attcagaacg ccgatcagct ggtacaggcg 3000

attccctatg tacaccatgc ttggttaccg gatgctccag gtatgaacta tgatgtatat 3060

caagggttaa acgcacgtat catgcaggcg tacaatttat atgatgcacg aaatgtcata 3120

ataaatggtg actttacaca aggactacaa ggatggcacg caacaggaaa agcagcggta 3180

caacaaatag atggagcttc agtattagtt ctatcaaact ggagtgccga ggtatctcag 3240

aatctgcatg cccaagatca tcatggatat atgttacgtg tgattgccaa aaaagaaggt 3300

cctggaaaag ggtatgtaat gatgatggat tttaatggaa agcaggaaac acttacgttc 3360

acttcttgtg aagaaggata tataacaaaa acaatagagg tattcccgga aagtgatcga 3420

atacgaattg aaatgggaga aacagagggt acgttttatg tagatagcat cgagttgctt 3480

tgtatgcaag gatatgctag cgataataac ccgcacacgg gtaatatgta tgagcaaagt 3540

tataatggaa attataatca aaatactagc gatgtgtatc accaaggata tataaacaac 3600

tataaccaaa attctagtag tatgtataat caaaattata ttaacaatga tgacctgcat 3660

tccggttgca catgtaacca agggcataac tctggctgta catgtaatca aggatataac 3720

cgttag 3726

<210> 35

<211> 3726

<212> DNA

<213> 人工的

<220>

<223> 设计用于在植物细胞中表达的编码TIC868_11的合成核苷酸序列。

<400> 35

atgacgagca accggaagaa cgagaacgag atcatcaacg ccctctcgat ccctgctgtt 60

tcaaaccact ccgcgcagat gaacctgtcc accgacgcgc gcatcgagga ctccctctgc 120

atagccgagg gcaacaacat cgacccattc gtgtcggcca gcacggttca gaccggcatc 180

aacatcgcgg gccgtatcct cggcgtcctc ggtgtcccat tcgccggtca gatcgcgtcc 240

ttctactcgt tccttgtggg cgagctgtgg cctcgcggtc gtgacccgtg ggagatcttc 300

ctggagcatg tggagcagtt gatccggcag caagtcacgg agaacacccg cgatactgct 360

ctggccaggc tacagggcct gggaaactcc tttcgggcat accagcagtc actggaggac 420

tggttggaga acagggatga cgcgcgaaca cgctcggtac tctacaccca gtacatcgct 480

ctcgaactcg acttcctgaa cgctatgccg ctgttcgcca tcaggaacca ggaagttcca 540

ctccttatgg tgtacgccca ggccgccaac ttacatctgc tcctgctgcg ggacgccagc 600

ctgttcggct ccgagttcgg actcacatct caagaaatcc agcgttacta cgagcgccaa 660

gtggagaaga cccgtgagta cagtgactac tgcgctcgat ggtacaacac agggctcaac 720

aacctgcgcg gcaccaacgc tgagtcatgg ctccgttaca accagttccg ccgcgacttg 780

actttgggtg tcctagacct ggtggcgcta ttcccgtctt acgacacacg ggtgtaccca 840

atgaacacta gcgcgcaact cacgcgggag atctacacag acccaatcgg ccggacgaac 900

gcaccctccg gtttcgcatc cacgaattgg ttcaacaaca acgcaccctc cttctcggca 960

atcgaggccg ccgtcatccg ccctcctcac ctgctcgact ttcccgagca gctcacgatc 1020

ttctccgtgc tctcacgctg gtccaacaca cagtacatga actactgggt cgggcaccga 1080

ttggagagta ggacgatccg tggcagcttg agcaccagta cccacggcaa caccaacacc 1140

tccatcaacc cagttacgct acagttcacg agccgcgacg tttaccggac tgagtcgttc 1200

gcgggcatta acatccttct gacaacgccc gtcaacggcg tcccgtgggc ccggttcaac 1260

tggcgtaacc cgttgaactc cctgcgcggg tcattgctct acaccatcgg gtacacgggc 1320

gtcggcaccc agctcttcga cagtgaaact gagctgccgc ccgagaccac ggaacgcccg 1380

aactacgagt cctacagcca ccgcctgtcc aacatccggc tcatctctgg caacacgctg 1440

cgtgcgccgg tgtactcctg gacacaccgc agcgccgacc ggaccaacac gatctcttcc 1500

gactccatta accagatccc gctcgtgaag ggcttccgtg tgtggggtgg cacgagcgtc 1560

atcaccggtc cgggcttcac cggtggagac atactgcggc gcaacacttt cggcgacttc 1620

gtttcgttgc aagtgaacat caactcgccg atcacccagc gttaccgtct gaggttccgc 1680

tacgcttcaa gccgcgacgc gagggtcatt gtcctgaccg gagccgcgtc cacaggcgtg 1740

ggaggccaag tctcagtcaa catgcctctc cagaagacga tggagatagg cgagaacttg 1800

actagccgaa ccttccggta cactgatttc tcgaaccctt tctcattcag agcgaaccct 1860

gacatcattg ggatctccga gcaaccgctg ttcggtgctg gctccatcag ctctggcgaa 1920

ctgtacatcg acaagattga gatcatcctg gcggatgcga cggggactac cacctacgag 1980

tacgaggaga agcagaatct cgagaaggct cagaaggctc tgaacgctct gttcactgac 2040

gggaccaacg gctacctcca gatggacgcc actgactacg acatcaacca gacagctaac 2100

ctgattgagt gtgtgagtga cgaactgtac gctaaggaga agatcgtact cctggacgag 2160

gtgaagtacg ctaagcgcct gagcattagc cgtaacctgc tgctgaacga cgatctggag 2220

ttcagcgacg gctttggcga gaacggctgg accaccagcg acaacatctc catccaggcc 2280

gacaatccac tcttcaaagg caactacctc aagatgttcg gagccaggga catcgacggc 2340

accctctttc cgacctacct ctaccagaag atcgacgagt cccgcctcaa accctacacc 2400

cgctacaggg tgcgcggctt cgtgggcagc agcaagaacc tcaagctcgt ggtcacacgg 2460

tatgagaagg agatcgacgc catcatgaac gtgcccaacg atctcgccca catgcagctc 2520

aatccatcct gcggcgacta ccggtgcgag tccagctccc agttcctcgt gaaccaggtg 2580

caccctactc cgaccgctgg ctatgccctg gacatgtacg cctgccctag ttcctccgac 2640

aagaagcaca tcatgtgcca cgaccgtcat ccgttcgact tccacatcga caccggcgaa 2700

ctgaacccga acaccaacct gggcatcgac gtactgttca agatttccaa cccgaacggg 2760

tacgccacct tgggcaacct ggaggtcatc gaagaaggcc cgctgaccga cgaggccctg 2820

gtccacgtca aacagaagga gaagaagtgg cggcagcaca tggagaagaa gcggatggag 2880

actcaacaag cctacgaccc ggccaagcaa gctgtggacg ctctgttcac caacgagcaa 2940

gagcttgact accacactac tcttgaccac atccagaatg ctgaccagct tgtccaggct 3000

attccgtacg tccaccacgc ttggctaccg gacgctccag ggatgaacta cgatgtgtac 3060

cagggtctga acgcgcggat catgcaagcg tacaacctgt acgacgcgcg taacgtcatc 3120

atcaacggtg acttcactca gggtcttcaa ggttggcacg cgactggcaa agcggcagtc 3180

cagcagattg atggtgcgtc tgttcttgtg ttgagcaact ggtctgcgga ggtttctcag 3240

aacctgcacg cacaggatca ccacggctac atgctgaggg tgattgctaa gaaggagggc 3300

cctggcaaag gctacgtcat gatgatggac ttcaacggaa agcaagaaac cctgaccttc 3360

actagctgtg aggagggcta catcactaag accattgagg tctttccgga gtctgaccgc 3420

atccggatcg agatgggcga gaccgaaggc acgttctacg tggactccat cgaactcctc 3480

tgcatgcaag gctacgcctc cgacaacaac ccacacacgg gcaacatgta cgagcagtcc 3540

tacaacggga actacaacca gaacacctcc gatgtgtacc atcagggcta catcaacaac 3600

tacaaccaga acagcagcag catgtacaac cagaactaca tcaacaacga tgacttgcac 3660

tcgggttgca cctgcaacca gggtcacaac agtgggtgca cgtgcaacca gggatacaac 3720

cgttga 3726

<210> 36

<211> 1241

<212> PRT

<213> 人工的

<220>

<223> 嵌合蛋白质变体TIC868_11的氨基酸序列。

<400> 36

Met Thr Ser Asn Arg Lys Asn Glu Asn Glu Ile Ile Asn Ala Leu Ser

1 5 10 15

Ile Pro Ala Val Ser Asn His Ser Ala Gln Met Asn Leu Ser Thr Asp

20 25 30

Ala Arg Ile Glu Asp Ser Leu Cys Ile Ala Glu Gly Asn Asn Ile Asp

35 40 45

Pro Phe Val Ser Ala Ser Thr Val Gln Thr Gly Ile Asn Ile Ala Gly

50 55 60

Arg Ile Leu Gly Val Leu Gly Val Pro Phe Ala Gly Gln Ile Ala Ser

65 70 75 80

Phe Tyr Ser Phe Leu Val Gly Glu Leu Trp Pro Arg Gly Arg Asp Pro

85 90 95

Trp Glu Ile Phe Leu Glu His Val Glu Gln Leu Ile Arg Gln Gln Val

100 105 110

Thr Glu Asn Thr Arg Asp Thr Ala Leu Ala Arg Leu Gln Gly Leu Gly

115 120 125

Asn Ser Phe Arg Ala Tyr Gln Gln Ser Leu Glu Asp Trp Leu Glu Asn

130 135 140

Arg Asp Asp Ala Arg Thr Arg Ser Val Leu Tyr Thr Gln Tyr Ile Ala

145 150 155 160

Leu Glu Leu Asp Phe Leu Asn Ala Met Pro Leu Phe Ala Ile Arg Asn

165 170 175

Gln Glu Val Pro Leu Leu Met Val Tyr Ala Gln Ala Ala Asn Leu His

180 185 190

Leu Leu Leu Leu Arg Asp Ala Ser Leu Phe Gly Ser Glu Phe Gly Leu

195 200 205

Thr Ser Gln Glu Ile Gln Arg Tyr Tyr Glu Arg Gln Val Glu Lys Thr

210 215 220

Arg Glu Tyr Ser Asp Tyr Cys Ala Arg Trp Tyr Asn Thr Gly Leu Asn

225 230 235 240

Asn Leu Arg Gly Thr Asn Ala Glu Ser Trp Leu Arg Tyr Asn Gln Phe

245 250 255

Arg Arg Asp Leu Thr Leu Gly Val Leu Asp Leu Val Ala Leu Phe Pro

260 265 270

Ser Tyr Asp Thr Arg Val Tyr Pro Met Asn Thr Ser Ala Gln Leu Thr

275 280 285

Arg Glu Ile Tyr Thr Asp Pro Ile Gly Arg Thr Asn Ala Pro Ser Gly

290 295 300

Phe Ala Ser Thr Asn Trp Phe Asn Asn Asn Ala Pro Ser Phe Ser Ala

305 310 315 320

Ile Glu Ala Ala Val Ile Arg Pro Pro His Leu Leu Asp Phe Pro Glu

325 330 335

Gln Leu Thr Ile Phe Ser Val Leu Ser Arg Trp Ser Asn Thr Gln Tyr

340 345 350

Met Asn Tyr Trp Val Gly His Arg Leu Glu Ser Arg Thr Ile Arg Gly

355 360 365

Ser Leu Ser Thr Ser Thr His Gly Asn Thr Asn Thr Ser Ile Asn Pro

370 375 380

Val Thr Leu Gln Phe Thr Ser Arg Asp Val Tyr Arg Thr Glu Ser Phe

385 390 395 400

Ala Gly Ile Asn Ile Leu Leu Thr Thr Pro Val Asn Gly Val Pro Trp

405 410 415

Ala Arg Phe Asn Trp Arg Asn Pro Leu Asn Ser Leu Arg Gly Ser Leu

420 425 430

Leu Tyr Thr Ile Gly Tyr Thr Gly Val Gly Thr Gln Leu Phe Asp Ser

435 440 445

Glu Thr Glu Leu Pro Pro Glu Thr Thr Glu Arg Pro Asn Tyr Glu Ser

450 455 460

Tyr Ser His Arg Leu Ser Asn Ile Arg Leu Ile Ser Gly Asn Thr Leu

465 470 475 480

Arg Ala Pro Val Tyr Ser Trp Thr His Arg Ser Ala Asp Arg Thr Asn

485 490 495

Thr Ile Ser Ser Asp Ser Ile Asn Gln Ile Pro Leu Val Lys Gly Phe

500 505 510

Arg Val Trp Gly Gly Thr Ser Val Ile Thr Gly Pro Gly Phe Thr Gly

515 520 525

Gly Asp Ile Leu Arg Arg Asn Thr Phe Gly Asp Phe Val Ser Leu Gln

530 535 540

Val Asn Ile Asn Ser Pro Ile Thr Gln Arg Tyr Arg Leu Arg Phe Arg

545 550 555 560

Tyr Ala Ser Ser Arg Asp Ala Arg Val Ile Val Leu Thr Gly Ala Ala

565 570 575

Ser Thr Gly Val Gly Gly Gln Val Ser Val Asn Met Pro Leu Gln Lys

580 585 590

Thr Met Glu Ile Gly Glu Asn Leu Thr Ser Arg Thr Phe Arg Tyr Thr

595 600 605

Asp Phe Ser Asn Pro Phe Ser Phe Arg Ala Asn Pro Asp Ile Ile Gly

610 615 620

Ile Ser Glu Gln Pro Leu Phe Gly Ala Gly Ser Ile Ser Ser Gly Glu

625 630 635 640

Leu Tyr Ile Asp Lys Ile Glu Ile Ile Leu Ala Asp Ala Thr Gly Thr

645 650 655

Thr Thr Tyr Glu Tyr Glu Glu Lys Gln Asn Leu Glu Lys Ala Gln Lys

660 665 670

Ala Leu Asn Ala Leu Phe Thr Asp Gly Thr Asn Gly Tyr Leu Gln Met

675 680 685

Asp Ala Thr Asp Tyr Asp Ile Asn Gln Thr Ala Asn Leu Ile Glu Cys

690 695 700

Val Ser Asp Glu Leu Tyr Ala Lys Glu Lys Ile Val Leu Leu Asp Glu

705 710 715 720

Val Lys Tyr Ala Lys Arg Leu Ser Ile Ser Arg Asn Leu Leu Leu Asn

725 730 735

Asp Asp Leu Glu Phe Ser Asp Gly Phe Gly Glu Asn Gly Trp Thr Thr

740 745 750

Ser Asp Asn Ile Ser Ile Gln Ala Asp Asn Pro Leu Phe Lys Gly Asn

755 760 765

Tyr Leu Lys Met Phe Gly Ala Arg Asp Ile Asp Gly Thr Leu Phe Pro

770 775 780

Thr Tyr Leu Tyr Gln Lys Ile Asp Glu Ser Arg Leu Lys Pro Tyr Thr

785 790 795 800

Arg Tyr Arg Val Arg Gly Phe Val Gly Ser Ser Lys Asn Leu Lys Leu

805 810 815

Val Val Thr Arg Tyr Glu Lys Glu Ile Asp Ala Ile Met Asn Val Pro

820 825 830

Asn Asp Leu Ala His Met Gln Leu Asn Pro Ser Cys Gly Asp Tyr Arg

835 840 845

Cys Glu Ser Ser Ser Gln Phe Leu Val Asn Gln Val His Pro Thr Pro

850 855 860

Thr Ala Gly Tyr Ala Leu Asp Met Tyr Ala Cys Pro Ser Ser Ser Asp

865 870 875 880

Lys Lys His Ile Met Cys His Asp Arg His Pro Phe Asp Phe His Ile

885 890 895

Asp Thr Gly Glu Leu Asn Pro Asn Thr Asn Leu Gly Ile Asp Val Leu

900 905 910

Phe Lys Ile Ser Asn Pro Asn Gly Tyr Ala Thr Leu Gly Asn Leu Glu

915 920 925

Val Ile Glu Glu Gly Pro Leu Thr Asp Glu Ala Leu Val His Val Lys

930 935 940

Gln Lys Glu Lys Lys Trp Arg Gln His Met Glu Lys Lys Arg Met Glu

945 950 955 960

Thr Gln Gln Ala Tyr Asp Pro Ala Lys Gln Ala Val Asp Ala Leu Phe

965 970 975

Thr Asn Glu Gln Glu Leu Asp Tyr His Thr Thr Leu Asp His Ile Gln

980 985 990

Asn Ala Asp Gln Leu Val Gln Ala Ile Pro Tyr Val His His Ala Trp

995 1000 1005

Leu Pro Asp Ala Pro Gly Met Asn Tyr Asp Val Tyr Gln Gly Leu

1010 1015 1020

Asn Ala Arg Ile Met Gln Ala Tyr Asn Leu Tyr Asp Ala Arg Asn

1025 1030 1035

Val Ile Ile Asn Gly Asp Phe Thr Gln Gly Leu Gln Gly Trp His

1040 1045 1050

Ala Thr Gly Lys Ala Ala Val Gln Gln Ile Asp Gly Ala Ser Val

1055 1060 1065

Leu Val Leu Ser Asn Trp Ser Ala Glu Val Ser Gln Asn Leu His

1070 1075 1080

Ala Gln Asp His His Gly Tyr Met Leu Arg Val Ile Ala Lys Lys

1085 1090 1095

Glu Gly Pro Gly Lys Gly Tyr Val Met Met Met Asp Phe Asn Gly

1100 1105 1110

Lys Gln Glu Thr Leu Thr Phe Thr Ser Cys Glu Glu Gly Tyr Ile

1115 1120 1125

Thr Lys Thr Ile Glu Val Phe Pro Glu Ser Asp Arg Ile Arg Ile

1130 1135 1140

Glu Met Gly Glu Thr Glu Gly Thr Phe Tyr Val Asp Ser Ile Glu

1145 1150 1155

Leu Leu Cys Met Gln Gly Tyr Ala Ser Asp Asn Asn Pro His Thr

1160 1165 1170

Gly Asn Met Tyr Glu Gln Ser Tyr Asn Gly Asn Tyr Asn Gln Asn

1175 1180 1185

Thr Ser Asp Val Tyr His Gln Gly Tyr Ile Asn Asn Tyr Asn Gln

1190 1195 1200

Asn Ser Ser Ser Met Tyr Asn Gln Asn Tyr Ile Asn Asn Asp Asp

1205 1210 1215

Leu His Ser Gly Cys Thr Cys Asn Gln Gly His Asn Ser Gly Cys

1220 1225 1230

Thr Cys Asn Gln Gly Tyr Asn Arg

1235 1240

<210> 37

<211> 3468

<212> DNA

<213> 人工的

<220>

<223> 用于在细菌细胞中表达的编码TIC868_12的重组核苷酸序列。

<400> 37

atgacttcaa ataggaaaaa tgagaatgaa attataaatg ctttatcgat tccagctgta 60

tcgaatcatt ccgcacaaat gaatctatca accgatgctc gtattgagga tagcttgtgt 120

atagccgagg ggaacaatat cgatccattt gttagcgcat caacagtcca aacgggtatt 180

aacatagctg gtagaatact aggtgtatta ggcgtaccgt ttgctggaca aatagctagt 240

ttttatagtt ttcttgttgg tgaattatgg ccccgcggca gagatccttg ggaaattttc 300

ctagaacatg tcgaacaact tataagacaa caagtaacag aaaatactag ggatacggct 360

cttgctcgat tacaaggttt aggaaattcc tttagagcct atcaacagtc acttgaagat 420

tggctagaaa accgtgatga tgcaagaacg agaagtgttc tttataccca atatatagcc 480

ttagaacttg attttcttaa tgcgatgccg cttttcgcaa ttagaaacca agaagttcca 540

ttattaatgg tatatgctca agctgcaaat ttacacctat tattattgag agatgcctct 600

ctttttggta gtgaatttgg gcttacatcc caagaaattc aacgttatta tgagcgccaa 660

gtggaaaaaa cgagagaata ttctgattat tgcgcaagat ggtataatac gggtttaaat 720

aatttgagag ggacaaatgc tgaaagttgg ttgcgatata atcaattccg tagagactta 780

acgctaggag tattagatct agtggcacta ttcccaagct atgacacgcg tgtttatcca 840

atgaatacca gtgctcaatt aacaagagaa atttatacag atccaattgg gagaacaaat 900

gcaccttcag gatttgcaag tacgaattgg tttaataata atgcaccatc gttttctgcc 960

atagaggctg ccgttattag gcctccgcat ctacttgatt ttccagaaca gcttacaatt 1020

ttcagcgtat taagtcgatg gagtaatact caatatatga attactgggt gggacataga 1080

cttgaatcgc gaacaataag ggggtcatta agtacctcga cacacggaaa taccaatact 1140

tctattaatc ctgtaacatt acagttcaca tctcgagacg tttatagaac agaatcattt 1200

gcagggataa atatacttct aactactcct gtgaatggag taccttgggc tagatttaat 1260

tggagaaatc ccctgaattc tcttagaggt agccttctct atactatagg gtatactgga 1320

gtggggacac aactatttga ttcagaaact gaattaccac cagaaacaac agaacgacca 1380

aattatgaat cttacagtca tagattatct aatataagac taatatcagg aaacactttg 1440

agagcaccag tatattcttg gacgcaccgt agtgcagatc gtacaaatac cattagttca 1500

gatagcatta atcaaatacc tttagtgaaa ggatttagag tttggggggg cacctctgtc 1560

attacaggac caggatttac aggaggggat atccttcgaa gaaatacctt tggtgatttt 1620

gtatctctac aagtcaatat taattcacca attacccaaa gataccgttt aagatttcgt 1680

tacgcttcca gtagggatgc acgagttata gtattaacag gagcggcatc cacaggagtg 1740

ggaggccaag ttagtgtaaa tatgcctctt cagaaaacta tggaaatagg ggagaactta 1800

acatctagaa catttagata taccgatttt agtaatcctt tttcatttag agctaatcca 1860

gatataattg ggataagtga acaacctcta tttggtgcag gttctattag tagcggtgaa 1920

ctttatatag ataaaattga aattattcta gcagatgcaa caaatccgac gcgagaggcg 1980

gaagaggatc tagaagcagc gaagaaagcg gtggcgagct tgtttacacg tacaagggac 2040

ggattacaag taaatgtgac agattatcaa gtcgatcaag cggcaaattt agtgtcatgc 2100

ttatcagatg aacaatatgg gcatgacaaa aagatgttat tggaagcggt aagagcggca 2160

aaacgcctca gccgagaacg caacttactt caggatccag attttaatac aatcaatagt 2220

acagaagaaa atggatggaa agcaagtaac ggcgttacta ttagcgaggg cggtccattc 2280

tataaaggcc gtgcgcttca gctagcaagc gcaagagaaa attacccaac atacatttat 2340

caaaaagtaa atgcatcaga gttaaagccg tatacacgtt atagactgga tgggttcgtg 2400

aagagtagtc aagatttaga aattgatctc attcaccatc ataaagtcca tctcgtgaaa 2460

aatgtaccag ataatttagt atccgatact tactcggatg gttcttgcag tggaatgaat 2520

cgatgtgagg aacaacagat ggtaaatgcg caactggaaa cagaacatca tcatccgatg 2580

gattgctgtg aagcggctca aacacatgag ttttcttcct atattaatac aggcgatcta 2640

aattcaagtg tagatcaagg catttgggtt gtattgaaag ttcgaacaac cgatggttat 2700

gcgacgctag gaaatcttga attggtagag gtcggaccgt tatcgggtga atctctagaa 2760

cgtgaacaaa gggataatgc gaaatggagt gcagagctag gaagaaagcg tgcagaaaca 2820

gatcgcgtgt atcaagatgc caaacaatcc atcaatcatt tatttgtgga ttatcaagat 2880

caacaattaa atccagaaat agggatggca gatattattg acgctcaaaa tcttgtcgca 2940

tcaatttcag atgtgtatag cgatgcagta ctgcaaatcc ctggaattaa ctatgagatt 3000

tacacagagc tatccaatcg cttacaacaa gcatcgtatc tgtatacgtc tcgaaatgcg 3060

gtgcaaaatg gggactttaa cagcggtcta gatagttgga atgcaacagg gggggctacg 3120

gtacaacagg atggcaatac gcatttctta gttctttctc attgggatgc acaagtttct 3180

caacaattta gagtgcagcc gaattgtaaa tatgtattac gtgtaacagc agagaaagta 3240

ggcggcggag acggatacgt gacaatccgg gatggtgctc atcatacaga aaagcttaca 3300

tttaatgcat gtgattatga tataaatggc acgtacgtga ctgataatac gtatctaaca 3360

aaagaagtgg tattctattc acatacagaa cacatgtggg tagaggtaag tgaaacagaa 3420

ggtgcatttc atatagatag tattgaattc gttgaaacag aaaagtag 3468

<210> 38

<211> 3468

<212> DNA

<213> 人工的

<220>

<223> 设计用于在植物细胞中表达的编码TIC868_12的合成核苷酸序列。

<400> 38

atgacgagca accggaagaa cgagaacgag atcatcaacg ccctctcgat ccctgctgtt 60

tcaaaccact ccgcgcagat gaacctgtcc accgacgcgc gcatcgagga ctccctctgc 120

atagccgagg gcaacaacat cgacccattc gtgtcggcca gcacggttca gaccggcatc 180

aacatcgcgg gccgtatcct cggcgtcctc ggtgtcccat tcgccggtca gatcgcgtcc 240

ttctactcgt tccttgtggg cgagctgtgg cctcgcggtc gtgacccgtg ggagatcttc 300

ctggagcatg tggagcagtt gatccggcag caagtcacgg agaacacccg cgatactgct 360

ctggccaggc tacagggcct gggaaactcc tttcgggcat accagcagtc actggaggac 420

tggttggaga acagggatga cgcgcgaaca cgctcggtac tctacaccca gtacatcgct 480

ctcgaactcg acttcctgaa cgctatgccg ctgttcgcca tcaggaacca ggaagttcca 540

ctccttatgg tgtacgccca ggccgccaac ttacatctgc tcctgctgcg ggacgccagc 600

ctgttcggct ccgagttcgg actcacatct caagaaatcc agcgttacta cgagcgccaa 660

gtggagaaga cccgtgagta cagtgactac tgcgctcgat ggtacaacac agggctcaac 720

aacctgcgcg gcaccaacgc tgagtcatgg ctccgttaca accagttccg ccgcgacttg 780

actttgggtg tcctagacct ggtggcgcta ttcccgtctt acgacacacg ggtgtaccca 840

atgaacacta gcgcgcaact cacgcgggag atctacacag acccaatcgg ccggacgaac 900

gcaccctccg gtttcgcatc cacgaattgg ttcaacaaca acgcaccctc cttctcggca 960

atcgaggccg ccgtcatccg ccctcctcac ctgctcgact ttcccgagca gctcacgatc 1020

ttctccgtgc tctcacgctg gtccaacaca cagtacatga actactgggt cgggcaccga 1080

ttggagagta ggacgatccg tggcagcttg agcaccagta cccacggcaa caccaacacc 1140

tccatcaacc cagttacgct acagttcacg agccgcgacg tttaccggac tgagtcgttc 1200

gcgggcatta acatccttct gacaacgccc gtcaacggcg tcccgtgggc ccggttcaac 1260

tggcgtaacc cgttgaactc cctgcgcggg tcattgctct acaccatcgg gtacacgggc 1320

gtcggcaccc agctcttcga cagtgaaact gagctgccgc ccgagaccac ggaacgcccg 1380

aactacgagt cctacagcca ccgcctgtcc aacatccggc tcatctctgg caacacgctg 1440

cgtgcgccgg tgtactcctg gacacaccgc agcgccgacc ggaccaacac gatctcttcc 1500

gactccatta accagatccc gctcgtgaag ggcttccgtg tgtggggtgg cacgagcgtc 1560

atcaccggtc cgggcttcac cggtggagac atactgcggc gcaacacttt cggcgacttc 1620

gtttcgttgc aagtgaacat caactcgccg atcacccagc gttaccgtct gaggttccgc 1680

tacgcttcaa gccgcgacgc gagggtcatt gtcctgaccg gagccgcgtc cacaggcgtg 1740

ggaggccaag tctcagtcaa catgcctctc cagaagacga tggagatagg cgagaacttg 1800

actagccgaa ccttccggta cactgatttc tcgaaccctt tctcattcag agcgaaccct 1860

gacatcattg ggatctccga gcaaccgctg ttcggtgctg gctccatcag ctctggcgaa 1920

ctgtacatcg acaagattga gatcatcctg gcggatgcga cgaacccgac gcgggaagct 1980

gaggaagact tggaagccgc caagaaagcg gtcgccagcc tgtttactcg gacgcgggac 2040

gggctccaag tgaatgtgac ggactatcaa gtggatcagg ccgctaacct cgtgtcatgc 2100

ctgagcgacg agcagtacgg tcacgacaag aaaatgctgc tggaggccgt ccgggccgcc 2160

aagcggctgt ccagggagcg taacctgcta caagatcccg actttaacac gatcaacagc 2220

acagaggaga atggctggaa ggccagcaac ggagttacga taagcgaggg cggtccgttc 2280

tacaagggtc gtgccctcca gctcgcctct gcaagggaga actatccaac ctacatctat 2340

cagaaggtga acgcatccga gcttaagccc tacacacgct accgcctgga cgggttcgtt 2400

aagtccagtc aagacctaga gatagacctc atccaccacc acaaagtgca tctggtcaag 2460

aacgttcccg ataatctcgt gagcgatacc tactcagacg gctcatgctc tggcatgaac 2520

agatgtgagg agcaacagat ggttaatgct caactcgaaa ccgagcatca tcatcctatg 2580

gattgctgcg aggccgcgca gacccatgag ttcagctctt acatcaacac cggagacctc 2640

aacagtagcg tggatcaggg aatttgggtg gtgcttaaag tgcgtacaac cgacggctac 2700

gccaccctcg gcaaccttga gcttgtcgag gtcggaccac ttagcggcga gtccctggaa 2760

cgtgagcagc gggacaacgc caaatggagc gcagagctag ggcgcaaacg cgcggagacg 2820

gaccgggttt atcaggacgc gaagcagtcc atcaatcacc tcttcgtgga ttatcaggac 2880

cagcagctta atccagagat cggcatggcc gacatcatcg acgcccagaa cctagtagcg 2940

tcgatttccg atgtctattc cgacgccgtg cttcaaatac ctggcatcaa ctacgagatc 3000

tacacagagt tgtccaacag gctccagcaa gcgtcatacc tctacaccag ccgcaacgcc 3060

gtccagaatg gcgacttcaa ttccggacta gactcctgga acgccacggg cggagctacg 3120

gtgcaacaag acggcaacac ccacttcctc gtacttagcc actgggacgc tcaagtgagt 3180

cagcaattcc gggttcagcc gaactgcaag tacgtcctgc gcgtaacggc cgagaaggtt 3240

ggaggcggag acggctacgt taccatccgc gacggcgctc accacaccga gaaactgacg 3300

ttcaacgctt gtgactacga catcaacggc acttacgtga cggacaacac ctacctgacg 3360

aaggaggtgg tgttctattc tcacaccgag cacatgtggg ttgaggtcag cgagaccgag 3420

ggagccttcc acattgacag catcgagttc gtggagactg agaagtga 3468

<210> 39

<211> 1155

<212> PRT

<213> 人工的

<220>

<223> 嵌合蛋白质变体TIC868_12的氨基酸序列。

<400> 39

Met Thr Ser Asn Arg Lys Asn Glu Asn Glu Ile Ile Asn Ala Leu Ser

1 5 10 15

Ile Pro Ala Val Ser Asn His Ser Ala Gln Met Asn Leu Ser Thr Asp

20 25 30

Ala Arg Ile Glu Asp Ser Leu Cys Ile Ala Glu Gly Asn Asn Ile Asp

35 40 45

Pro Phe Val Ser Ala Ser Thr Val Gln Thr Gly Ile Asn Ile Ala Gly

50 55 60

Arg Ile Leu Gly Val Leu Gly Val Pro Phe Ala Gly Gln Ile Ala Ser

65 70 75 80

Phe Tyr Ser Phe Leu Val Gly Glu Leu Trp Pro Arg Gly Arg Asp Pro

85 90 95

Trp Glu Ile Phe Leu Glu His Val Glu Gln Leu Ile Arg Gln Gln Val

100 105 110

Thr Glu Asn Thr Arg Asp Thr Ala Leu Ala Arg Leu Gln Gly Leu Gly

115 120 125

Asn Ser Phe Arg Ala Tyr Gln Gln Ser Leu Glu Asp Trp Leu Glu Asn

130 135 140

Arg Asp Asp Ala Arg Thr Arg Ser Val Leu Tyr Thr Gln Tyr Ile Ala

145 150 155 160

Leu Glu Leu Asp Phe Leu Asn Ala Met Pro Leu Phe Ala Ile Arg Asn

165 170 175

Gln Glu Val Pro Leu Leu Met Val Tyr Ala Gln Ala Ala Asn Leu His

180 185 190

Leu Leu Leu Leu Arg Asp Ala Ser Leu Phe Gly Ser Glu Phe Gly Leu

195 200 205

Thr Ser Gln Glu Ile Gln Arg Tyr Tyr Glu Arg Gln Val Glu Lys Thr

210 215 220

Arg Glu Tyr Ser Asp Tyr Cys Ala Arg Trp Tyr Asn Thr Gly Leu Asn

225 230 235 240

Asn Leu Arg Gly Thr Asn Ala Glu Ser Trp Leu Arg Tyr Asn Gln Phe

245 250 255

Arg Arg Asp Leu Thr Leu Gly Val Leu Asp Leu Val Ala Leu Phe Pro

260 265 270

Ser Tyr Asp Thr Arg Val Tyr Pro Met Asn Thr Ser Ala Gln Leu Thr

275 280 285

Arg Glu Ile Tyr Thr Asp Pro Ile Gly Arg Thr Asn Ala Pro Ser Gly

290 295 300

Phe Ala Ser Thr Asn Trp Phe Asn Asn Asn Ala Pro Ser Phe Ser Ala

305 310 315 320

Ile Glu Ala Ala Val Ile Arg Pro Pro His Leu Leu Asp Phe Pro Glu

325 330 335

Gln Leu Thr Ile Phe Ser Val Leu Ser Arg Trp Ser Asn Thr Gln Tyr

340 345 350

Met Asn Tyr Trp Val Gly His Arg Leu Glu Ser Arg Thr Ile Arg Gly

355 360 365

Ser Leu Ser Thr Ser Thr His Gly Asn Thr Asn Thr Ser Ile Asn Pro

370 375 380

Val Thr Leu Gln Phe Thr Ser Arg Asp Val Tyr Arg Thr Glu Ser Phe

385 390 395 400

Ala Gly Ile Asn Ile Leu Leu Thr Thr Pro Val Asn Gly Val Pro Trp

405 410 415

Ala Arg Phe Asn Trp Arg Asn Pro Leu Asn Ser Leu Arg Gly Ser Leu

420 425 430

Leu Tyr Thr Ile Gly Tyr Thr Gly Val Gly Thr Gln Leu Phe Asp Ser

435 440 445

Glu Thr Glu Leu Pro Pro Glu Thr Thr Glu Arg Pro Asn Tyr Glu Ser

450 455 460

Tyr Ser His Arg Leu Ser Asn Ile Arg Leu Ile Ser Gly Asn Thr Leu

465 470 475 480

Arg Ala Pro Val Tyr Ser Trp Thr His Arg Ser Ala Asp Arg Thr Asn

485 490 495

Thr Ile Ser Ser Asp Ser Ile Asn Gln Ile Pro Leu Val Lys Gly Phe

500 505 510

Arg Val Trp Gly Gly Thr Ser Val Ile Thr Gly Pro Gly Phe Thr Gly

515 520 525

Gly Asp Ile Leu Arg Arg Asn Thr Phe Gly Asp Phe Val Ser Leu Gln

530 535 540

Val Asn Ile Asn Ser Pro Ile Thr Gln Arg Tyr Arg Leu Arg Phe Arg

545 550 555 560

Tyr Ala Ser Ser Arg Asp Ala Arg Val Ile Val Leu Thr Gly Ala Ala

565 570 575

Ser Thr Gly Val Gly Gly Gln Val Ser Val Asn Met Pro Leu Gln Lys

580 585 590

Thr Met Glu Ile Gly Glu Asn Leu Thr Ser Arg Thr Phe Arg Tyr Thr

595 600 605

Asp Phe Ser Asn Pro Phe Ser Phe Arg Ala Asn Pro Asp Ile Ile Gly

610 615 620

Ile Ser Glu Gln Pro Leu Phe Gly Ala Gly Ser Ile Ser Ser Gly Glu

625 630 635 640

Leu Tyr Ile Asp Lys Ile Glu Ile Ile Leu Ala Asp Ala Thr Asn Pro

645 650 655

Thr Arg Glu Ala Glu Glu Asp Leu Glu Ala Ala Lys Lys Ala Val Ala

660 665 670

Ser Leu Phe Thr Arg Thr Arg Asp Gly Leu Gln Val Asn Val Thr Asp

675 680 685

Tyr Gln Val Asp Gln Ala Ala Asn Leu Val Ser Cys Leu Ser Asp Glu

690 695 700

Gln Tyr Gly His Asp Lys Lys Met Leu Leu Glu Ala Val Arg Ala Ala

705 710 715 720

Lys Arg Leu Ser Arg Glu Arg Asn Leu Leu Gln Asp Pro Asp Phe Asn

725 730 735

Thr Ile Asn Ser Thr Glu Glu Asn Gly Trp Lys Ala Ser Asn Gly Val

740 745 750

Thr Ile Ser Glu Gly Gly Pro Phe Tyr Lys Gly Arg Ala Leu Gln Leu

755 760 765

Ala Ser Ala Arg Glu Asn Tyr Pro Thr Tyr Ile Tyr Gln Lys Val Asn

770 775 780

Ala Ser Glu Leu Lys Pro Tyr Thr Arg Tyr Arg Leu Asp Gly Phe Val

785 790 795 800

Lys Ser Ser Gln Asp Leu Glu Ile Asp Leu Ile His His His Lys Val

805 810 815

His Leu Val Lys Asn Val Pro Asp Asn Leu Val Ser Asp Thr Tyr Ser

820 825 830

Asp Gly Ser Cys Ser Gly Met Asn Arg Cys Glu Glu Gln Gln Met Val

835 840 845

Asn Ala Gln Leu Glu Thr Glu His His His Pro Met Asp Cys Cys Glu

850 855 860

Ala Ala Gln Thr His Glu Phe Ser Ser Tyr Ile Asn Thr Gly Asp Leu

865 870 875 880

Asn Ser Ser Val Asp Gln Gly Ile Trp Val Val Leu Lys Val Arg Thr

885 890 895

Thr Asp Gly Tyr Ala Thr Leu Gly Asn Leu Glu Leu Val Glu Val Gly

900 905 910

Pro Leu Ser Gly Glu Ser Leu Glu Arg Glu Gln Arg Asp Asn Ala Lys

915 920 925

Trp Ser Ala Glu Leu Gly Arg Lys Arg Ala Glu Thr Asp Arg Val Tyr

930 935 940

Gln Asp Ala Lys Gln Ser Ile Asn His Leu Phe Val Asp Tyr Gln Asp

945 950 955 960

Gln Gln Leu Asn Pro Glu Ile Gly Met Ala Asp Ile Ile Asp Ala Gln

965 970 975

Asn Leu Val Ala Ser Ile Ser Asp Val Tyr Ser Asp Ala Val Leu Gln

980 985 990

Ile Pro Gly Ile Asn Tyr Glu Ile Tyr Thr Glu Leu Ser Asn Arg Leu

995 1000 1005

Gln Gln Ala Ser Tyr Leu Tyr Thr Ser Arg Asn Ala Val Gln Asn

1010 1015 1020

Gly Asp Phe Asn Ser Gly Leu Asp Ser Trp Asn Ala Thr Gly Gly

1025 1030 1035

Ala Thr Val Gln Gln Asp Gly Asn Thr His Phe Leu Val Leu Ser

1040 1045 1050

His Trp Asp Ala Gln Val Ser Gln Gln Phe Arg Val Gln Pro Asn

1055 1060 1065

Cys Lys Tyr Val Leu Arg Val Thr Ala Glu Lys Val Gly Gly Gly

1070 1075 1080

Asp Gly Tyr Val Thr Ile Arg Asp Gly Ala His His Thr Glu Lys

1085 1090 1095

Leu Thr Phe Asn Ala Cys Asp Tyr Asp Ile Asn Gly Thr Tyr Val

1100 1105 1110

Thr Asp Asn Thr Tyr Leu Thr Lys Glu Val Val Phe Tyr Ser His

1115 1120 1125

Thr Glu His Met Trp Val Glu Val Ser Glu Thr Glu Gly Ala Phe

1130 1135 1140

His Ile Asp Ser Ile Glu Phe Val Glu Thr Glu Lys

1145 1150 1155

<210> 40

<211> 3732

<212> DNA

<213> 人工的

<220>

<223> 设计用于在植物细胞中表达的编码TIC868_13的合成核苷酸序列。

<400> 40

atgacgagca accggaagaa cgagaacgag atcatcaacg ccctctcgat ccctgctgtt 60

tcaaaccact ccgcgcagat gaacctgtcc accgacgcgc gcatcgagga ctccctctgc 120

atagccgagg gcaacaacat cgacccattc gtgtcggcca gcacggttca gaccggcatc 180

aacatcgcgg gccgtatcct cggcgtcctc ggtgtcccat tcgccggtca gatcgcgtcc 240

ttctactcgt tccttgtggg cgagctgtgg cctcgcggtc gtgacccgtg ggagatcttc 300

ctggagcatg tggagcagtt gatccggcag caagtcacgg agaacacccg cgatactgct 360

ctggccaggc tacagggcct gggaaactcc tttcgggcat accagcagtc actggaggac 420

tggttggaga acagggatga cgcgcgaaca cgctcggtac tctacaccca gtacatcgct 480

ctcgaactcg acttcctgaa cgctatgccg ctgttcgcca tcaggaacca ggaagttcca 540

ctccttatgg tgtacgccca ggccgccaac ttacatctgc tcctgctgcg ggacgccagc 600

ctgttcggct ccgagttcgg actcacatct caagaaatcc agcgttacta cgagcgccaa 660

gtggagaaga cccgtgagta cagtgactac tgcgctcgat ggtacaacac agggctcaac 720

aacctgcgcg gcaccaacgc tgagtcatgg ctccgttaca accagttccg ccgcgacttg 780

actttgggtg tcctagacct ggtggcgcta ttcccgtctt acgacacacg ggtgtaccca 840

atgaacacta gcgcgcaact cacgcgggag atctacacag acccaatcgg ccggacgaac 900

gcaccctccg gtttcgcatc cacgaattgg ttcaacaaca acgcaccctc cttctcggca 960

atcgaggccg ccgtcatccg ccctcctcac ctgctcgact ttcccgagca gctcacgatc 1020

ttctccgtgc tctcacgctg gtccaacaca cagtacatga actactgggt cgggcaccga 1080

ttggagagta ggacgatccg tggcagcttg agcaccagta cccacggcaa caccaacacc 1140

tccatcaacc cagttacgct acagttcacg agccgcgacg tttaccggac tgagtcgttc 1200

gcgggcatta acatccttct gacaacgccc gtcaacggcg tcccgtgggc ccggttcaac 1260

tggcgtaacc cgttgaactc cctgcgcggg tcattgctct acaccatcgg gtacacgggc 1320

gtcggcaccc agctcttcga cagtgaaact gagctgccgc ccgagaccac ggaacgcccg 1380

aactacgagt cctacagcca ccgcctgtcc aacatccggc tcatctctgg caacacgctg 1440

cgtgcgccgg tgtactcctg gacacaccgc agcgccgacc ggaccaacac gatctcttcc 1500

gactccatta accagatccc gctcgtgaag ggcttccgtg tgtggggtgg cacgagcgtc 1560

atcaccggtc cgggcttcac cggtggagac atactgcggc gcaacacttt cggcgacttc 1620

gtttcgttgc aagtgaacat caactcgccg atcacccagc gttaccgtct gaggttccgc 1680

tacgcttcaa gccgcgacgc gagggtcatt gtcctgaccg gagccgcgtc cacaggcgtg 1740

ggaggccaag tctcagtcaa catgcctctc cagaagacga tggagatagg cgagaacttg 1800

actagccgaa ccttccggta cactgatttc tcgaaccctt tctcattcag agcgaaccct 1860

gacatcattg ggatctccga gcaaccgctg ttcggtgctg gctccatcag ctctggcgaa 1920

ctgtacatcg acaagattga gatcatcctg gcggatgcga cgacggcgac cttcgaggcg 1980

gagtatgact tggagcgggc tcaggaggcc gtcaacgcgc tgttcacaaa caccaatcct 2040

cgccgcctca agacgggtgt gactgattac cacattgacg aggtctccaa cttggtcgcg 2100

tgtctgtccg atgagttctg cctggacgag aagcgggaac tgctggagaa ggtcaagtac 2160

gccaagcgcc tctccgacga aaggaacctc ctccaagatc ccaactttac ttccattaac 2220

aagcagccgg acttcatctc caccaacgag cagtccaact tcacctcaat ccacgagcag 2280

tcggagcacg ggtggtgggg cagcgagaac atcaccatcc aagagggcaa cgacgtcttc 2340

aaggagaact acgtgatcct gcccggcacc ttcaacgagt gttacccgac ctatctctac 2400

cagaagattg gcgaagcgga actcaaggct tacacccgtt accaactgag tggctacatt 2460

gaggactcac aagacctgga aatctacctg atccgctaca acgccaagca cgagaccctc 2520

gacgtgcctg gcacggagtc cgtctggccc ttgagcgtgg agtctcctat cggtcgttgc 2580

ggcgagccca atcgctgcgc tccgcacttt gagtggaatc ctgatttgga ttgctcctgc 2640

cgagacggtg agaaatgcgc ccaccactcg caccacttca gcctagacat cgacgtgggc 2700

tgcatcgacc tgcacgagaa cttgggcgtc tgggtcgtgt tcaagatcaa gacacaggag 2760

ggccatgctc ggcttgggaa cctggagttc atcgaggaga agccactgct gggtgaagcc 2820

ttgtcacggg tgaaacgcgc cgagaagaag tggcgggaca aacgggagaa gctccagttg 2880

gagacaaagc gtgtgtacac agaggccaag gaggccgtgg atgccttgtt cgtggacagt 2940

cagtacgaca ggctgcaagc ggacaccaac atcgggatga tccacgcggc tgataagctt 3000

gttcacagaa tccgcgaggc gtacctgtca gagcttagcg tgatcccagg cgtcaacgcc 3060

gaaatcttcg aggaactgga gggccgcatt atcacggcaa tctcacttta tgacgcgagg 3120

aatgtggtca agaacggtga cttcaacaac ggcttggcgt gttggaacgt taaagggcac 3180

gtggatgtac aacagtcaca ccacagaagt gtcttggtca tcccggagtg ggaggcggaa 3240

gtgagccagg ccgtccgggt ctgccctggg cgcggttaca tcctccgcgt gacagcgtac 3300

aaggagggct acggtgaggg ctgcgtgacg atccacgaga ttgagaacaa cacggacgag 3360

cttaagttca agaactgcga ggaggaggaa gtgtacccga cagacaccgg cacctgcaac 3420

gactacaccg cccaccaagg gaccgccgcc tgcaacagcc gcaacgcggg ctatgaagat 3480

gcgtacgagg ttgataccac cgcctcagtg aactacaaac cgacttatga ggaggagaca 3540

tacacggacg tcaggcgcga caaccattgt gagtacgacc gtggctacgt gaactatccg 3600

ccggtgccag cgggctacat gacgaaggag ctagaatact tccctgagac ggacaaggtg 3660

tggattgaaa tcggcgagac cgagggcaag tttatcgtgg attctgtcga gctgctgcta 3720

atggaggagt ag 3732

<210> 41

<211> 1243

<212> PRT

<213> 人工的

<220>

<223> 嵌合蛋白质变体TIC868_13的氨基酸序列。

<400> 41

Met Thr Ser Asn Arg Lys Asn Glu Asn Glu Ile Ile Asn Ala Leu Ser

1 5 10 15

Ile Pro Ala Val Ser Asn His Ser Ala Gln Met Asn Leu Ser Thr Asp

20 25 30

Ala Arg Ile Glu Asp Ser Leu Cys Ile Ala Glu Gly Asn Asn Ile Asp

35 40 45

Pro Phe Val Ser Ala Ser Thr Val Gln Thr Gly Ile Asn Ile Ala Gly

50 55 60

Arg Ile Leu Gly Val Leu Gly Val Pro Phe Ala Gly Gln Ile Ala Ser

65 70 75 80

Phe Tyr Ser Phe Leu Val Gly Glu Leu Trp Pro Arg Gly Arg Asp Pro

85 90 95

Trp Glu Ile Phe Leu Glu His Val Glu Gln Leu Ile Arg Gln Gln Val

100 105 110

Thr Glu Asn Thr Arg Asp Thr Ala Leu Ala Arg Leu Gln Gly Leu Gly

115 120 125

Asn Ser Phe Arg Ala Tyr Gln Gln Ser Leu Glu Asp Trp Leu Glu Asn

130 135 140

Arg Asp Asp Ala Arg Thr Arg Ser Val Leu Tyr Thr Gln Tyr Ile Ala

145 150 155 160

Leu Glu Leu Asp Phe Leu Asn Ala Met Pro Leu Phe Ala Ile Arg Asn

165 170 175

Gln Glu Val Pro Leu Leu Met Val Tyr Ala Gln Ala Ala Asn Leu His

180 185 190

Leu Leu Leu Leu Arg Asp Ala Ser Leu Phe Gly Ser Glu Phe Gly Leu

195 200 205

Thr Ser Gln Glu Ile Gln Arg Tyr Tyr Glu Arg Gln Val Glu Lys Thr

210 215 220

Arg Glu Tyr Ser Asp Tyr Cys Ala Arg Trp Tyr Asn Thr Gly Leu Asn

225 230 235 240

Asn Leu Arg Gly Thr Asn Ala Glu Ser Trp Leu Arg Tyr Asn Gln Phe

245 250 255

Arg Arg Asp Leu Thr Leu Gly Val Leu Asp Leu Val Ala Leu Phe Pro

260 265 270

Ser Tyr Asp Thr Arg Val Tyr Pro Met Asn Thr Ser Ala Gln Leu Thr

275 280 285

Arg Glu Ile Tyr Thr Asp Pro Ile Gly Arg Thr Asn Ala Pro Ser Gly

290 295 300

Phe Ala Ser Thr Asn Trp Phe Asn Asn Asn Ala Pro Ser Phe Ser Ala

305 310 315 320

Ile Glu Ala Ala Val Ile Arg Pro Pro His Leu Leu Asp Phe Pro Glu

325 330 335

Gln Leu Thr Ile Phe Ser Val Leu Ser Arg Trp Ser Asn Thr Gln Tyr

340 345 350

Met Asn Tyr Trp Val Gly His Arg Leu Glu Ser Arg Thr Ile Arg Gly

355 360 365

Ser Leu Ser Thr Ser Thr His Gly Asn Thr Asn Thr Ser Ile Asn Pro

370 375 380

Val Thr Leu Gln Phe Thr Ser Arg Asp Val Tyr Arg Thr Glu Ser Phe

385 390 395 400

Ala Gly Ile Asn Ile Leu Leu Thr Thr Pro Val Asn Gly Val Pro Trp

405 410 415

Ala Arg Phe Asn Trp Arg Asn Pro Leu Asn Ser Leu Arg Gly Ser Leu

420 425 430

Leu Tyr Thr Ile Gly Tyr Thr Gly Val Gly Thr Gln Leu Phe Asp Ser

435 440 445

Glu Thr Glu Leu Pro Pro Glu Thr Thr Glu Arg Pro Asn Tyr Glu Ser

450 455 460

Tyr Ser His Arg Leu Ser Asn Ile Arg Leu Ile Ser Gly Asn Thr Leu

465 470 475 480

Arg Ala Pro Val Tyr Ser Trp Thr His Arg Ser Ala Asp Arg Thr Asn

485 490 495

Thr Ile Ser Ser Asp Ser Ile Asn Gln Ile Pro Leu Val Lys Gly Phe

500 505 510

Arg Val Trp Gly Gly Thr Ser Val Ile Thr Gly Pro Gly Phe Thr Gly

515 520 525

Gly Asp Ile Leu Arg Arg Asn Thr Phe Gly Asp Phe Val Ser Leu Gln

530 535 540

Val Asn Ile Asn Ser Pro Ile Thr Gln Arg Tyr Arg Leu Arg Phe Arg

545 550 555 560

Tyr Ala Ser Ser Arg Asp Ala Arg Val Ile Val Leu Thr Gly Ala Ala

565 570 575

Ser Thr Gly Val Gly Gly Gln Val Ser Val Asn Met Pro Leu Gln Lys

580 585 590

Thr Met Glu Ile Gly Glu Asn Leu Thr Ser Arg Thr Phe Arg Tyr Thr

595 600 605

Asp Phe Ser Asn Pro Phe Ser Phe Arg Ala Asn Pro Asp Ile Ile Gly

610 615 620

Ile Ser Glu Gln Pro Leu Phe Gly Ala Gly Ser Ile Ser Ser Gly Glu

625 630 635 640

Leu Tyr Ile Asp Lys Ile Glu Ile Ile Leu Ala Asp Ala Thr Thr Ala

645 650 655

Thr Phe Glu Ala Glu Tyr Asp Leu Glu Arg Ala Gln Glu Ala Val Asn

660 665 670

Ala Leu Phe Thr Asn Thr Asn Pro Arg Arg Leu Lys Thr Gly Val Thr

675 680 685

Asp Tyr His Ile Asp Glu Val Ser Asn Leu Val Ala Cys Leu Ser Asp

690 695 700

Glu Phe Cys Leu Asp Glu Lys Arg Glu Leu Leu Glu Lys Val Lys Tyr

705 710 715 720

Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn Phe

725 730 735

Thr Ser Ile Asn Lys Gln Pro Asp Phe Ile Ser Thr Asn Glu Gln Ser

740 745 750

Asn Phe Thr Ser Ile His Glu Gln Ser Glu His Gly Trp Trp Gly Ser

755 760 765

Glu Asn Ile Thr Ile Gln Glu Gly Asn Asp Val Phe Lys Glu Asn Tyr

770 775 780

Val Ile Leu Pro Gly Thr Phe Asn Glu Cys Tyr Pro Thr Tyr Leu Tyr

785 790 795 800

Gln Lys Ile Gly Glu Ala Glu Leu Lys Ala Tyr Thr Arg Tyr Gln Leu

805 810 815

Ser Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg

820 825 830

Tyr Asn Ala Lys His Glu Thr Leu Asp Val Pro Gly Thr Glu Ser Val

835 840 845

Trp Pro Leu Ser Val Glu Ser Pro Ile Gly Arg Cys Gly Glu Pro Asn

850 855 860

Arg Cys Ala Pro His Phe Glu Trp Asn Pro Asp Leu Asp Cys Ser Cys

865 870 875 880

Arg Asp Gly Glu Lys Cys Ala His His Ser His His Phe Ser Leu Asp

885 890 895

Ile Asp Val Gly Cys Ile Asp Leu His Glu Asn Leu Gly Val Trp Val

900 905 910

Val Phe Lys Ile Lys Thr Gln Glu Gly His Ala Arg Leu Gly Asn Leu

915 920 925

Glu Phe Ile Glu Glu Lys Pro Leu Leu Gly Glu Ala Leu Ser Arg Val

930 935 940

Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Lys Leu Gln Leu

945 950 955 960

Glu Thr Lys Arg Val Tyr Thr Glu Ala Lys Glu Ala Val Asp Ala Leu

965 970 975

Phe Val Asp Ser Gln Tyr Asp Arg Leu Gln Ala Asp Thr Asn Ile Gly

980 985 990

Met Ile His Ala Ala Asp Lys Leu Val His Arg Ile Arg Glu Ala Tyr

995 1000 1005

Leu Ser Glu Leu Ser Val Ile Pro Gly Val Asn Ala Glu Ile Phe

1010 1015 1020

Glu Glu Leu Glu Gly Arg Ile Ile Thr Ala Ile Ser Leu Tyr Asp

1025 1030 1035

Ala Arg Asn Val Val Lys Asn Gly Asp Phe Asn Asn Gly Leu Ala

1040 1045 1050

Cys Trp Asn Val Lys Gly His Val Asp Val Gln Gln Ser His His

1055 1060 1065

Arg Ser Val Leu Val Ile Pro Glu Trp Glu Ala Glu Val Ser Gln

1070 1075 1080

Ala Val Arg Val Cys Pro Gly Arg Gly Tyr Ile Leu Arg Val Thr

1085 1090 1095

Ala Tyr Lys Glu Gly Tyr Gly Glu Gly Cys Val Thr Ile His Glu

1100 1105 1110

Ile Glu Asn Asn Thr Asp Glu Leu Lys Phe Lys Asn Cys Glu Glu

1115 1120 1125

Glu Glu Val Tyr Pro Thr Asp Thr Gly Thr Cys Asn Asp Tyr Thr

1130 1135 1140

Ala His Gln Gly Thr Ala Ala Cys Asn Ser Arg Asn Ala Gly Tyr

1145 1150 1155

Glu Asp Ala Tyr Glu Val Asp Thr Thr Ala Ser Val Asn Tyr Lys

1160 1165 1170

Pro Thr Tyr Glu Glu Glu Thr Tyr Thr Asp Val Arg Arg Asp Asn

1175 1180 1185

His Cys Glu Tyr Asp Arg Gly Tyr Val Asn Tyr Pro Pro Val Pro

1190 1195 1200

Ala Gly Tyr Met Thr Lys Glu Leu Glu Tyr Phe Pro Glu Thr Asp

1205 1210 1215

Lys Val Trp Ile Glu Ile Gly Glu Thr Glu Gly Lys Phe Ile Val

1220 1225 1230

Asp Ser Val Glu Leu Leu Leu Met Glu Glu

1235 1240

<210> 42

<211> 3702

<212> DNA

<213> 人工的

<220>

<223> 设计用于在植物细胞中表达的编码TIC868_14的合成核苷酸序列。

<400> 42

atgacgagca accggaagaa cgagaacgag atcatcaacg ccctctcgat ccctgctgtt 60

tcaaaccact ccgcgcagat gaacctgtcc accgacgcgc gcatcgagga ctccctctgc 120

atagccgagg gcaacaacat cgacccattc gtgtcggcca gcacggttca gaccggcatc 180

aacatcgcgg gccgtatcct cggcgtcctc ggtgtcccat tcgccggtca gatcgcgtcc 240

ttctactcgt tccttgtggg cgagctgtgg cctcgcggtc gtgacccgtg ggagatcttc 300

ctggagcatg tggagcagtt gatccggcag caagtcacgg agaacacccg cgatactgct 360

ctggccaggc tacagggcct gggaaactcc tttcgggcat accagcagtc actggaggac 420

tggttggaga acagggatga cgcgcgaaca cgctcggtac tctacaccca gtacatcgct 480

ctcgaactcg acttcctgaa cgctatgccg ctgttcgcca tcaggaacca ggaagttcca 540

ctccttatgg tgtacgccca ggccgccaac ttacatctgc tcctgctgcg ggacgccagc 600

ctgttcggct ccgagttcgg actcacatct caagaaatcc agcgttacta cgagcgccaa 660

gtggagaaga cccgtgagta cagtgactac tgcgctcgat ggtacaacac agggctcaac 720

aacctgcgcg gcaccaacgc tgagtcatgg ctccgttaca accagttccg ccgcgacttg 780

actttgggtg tcctagacct ggtggcgcta ttcccgtctt acgacacacg ggtgtaccca 840

atgaacacta gcgcgcaact cacgcgggag atctacacag acccaatcgg ccggacgaac 900

gcaccctccg gtttcgcatc cacgaattgg ttcaacaaca acgcaccctc cttctcggca 960

atcgaggccg ccgtcatccg ccctcctcac ctgctcgact ttcccgagca gctcacgatc 1020

ttctccgtgc tctcacgctg gtccaacaca cagtacatga actactgggt cgggcaccga 1080

ttggagagta ggacgatccg tggcagcttg agcaccagta cccacggcaa caccaacacc 1140

tccatcaacc cagttacgct acagttcacg agccgcgacg tttaccggac tgagtcgttc 1200

gcgggcatta acatccttct gacaacgccc gtcaacggcg tcccgtgggc ccggttcaac 1260

tggcgtaacc cgttgaactc cctgcgcggg tcattgctct acaccatcgg gtacacgggc 1320

gtcggcaccc agctcttcga cagtgaaact gagctgccgc ccgagaccac ggaacgcccg 1380

aactacgagt cctacagcca ccgcctgtcc aacatccggc tcatctctgg caacacgctg 1440

cgtgcgccgg tgtactcctg gacacaccgc agcgccgacc ggaccaacac gatctcttcc 1500

gactccatta accagatccc gctcgtgaag ggcttccgtg tgtggggtgg cacgagcgtc 1560

atcaccggtc cgggcttcac cggtggagac atactgcggc gcaacacttt cggcgacttc 1620

gtttcgttgc aagtgaacat caactcgccg atcacccagc gttaccgtct gaggttccgc 1680

tacgcttcaa gccgcgacgc gagggtcatt gtcctgaccg gagccgcgtc cacaggcgtg 1740

ggaggccaag tctcagtcaa catgcctctc cagaagacga tggagatagg cgagaacttg 1800

actagccgaa ccttccggta cactgatttc tcgaaccctt tctcattcag agcgaaccct 1860

gacatcattg ggatctccga gcaaccgctg ttcggtgctg gctccatcag ctctggcgaa 1920

ctgtacatcg acaagattga gatcatcctg gcggatgcga cgaccgcgac gtttgaagct 1980

gaatccgacc tcgagcgtgc gcgcaaggcg gtgaacgctc tgttcacgag caccaaccct 2040

cgtggcttga agacggatgt gacggactac cacatcgacc aagtctcgaa cctcgtggag 2100

tgcctgagcg acgagttctg tcttgacaag aagcgcgagc tgctggagga ggtgaagtac 2160

gccaagcgcc tctccgatga gcgcaacctg ctccaagatc ctaccttcac gtcgatttcc 2220

ggccaaaccg accgtggatg gatcggctcg actggcatct ccatccaggg cggcgacgac 2280

atcttcaagg agaactatgt tcggctgccg ggcacggtgg acgagtgtta cccgacgtac 2340

ctctaccaga agatagacga gagtcaactc aagtcctaca cgcggtatca gttacgtggc 2400

tacattgaag actcccagga cttggaaatc tatctcatac ggtacaacgc caagcacgag 2460

accttaagcg tgccgggaac ggagtcgccc tggccaagct ctggcgtgta cccttccggt 2520

aggtgcggcg agcccaaccg ctgtgcacct cgaatcgaat ggaacccgga ccttgactgc 2580

tcttgccggt acggcgagaa gtgcgtccat cattctcacc acttcagctt ggacattgac 2640

gtcggctgca ccgacctcaa tgaagacctc ggagtgtggg tcatcttcaa gatcaagaca 2700

caggacgggc acgcgaaact aggaaacctg gagttcatcg aggagaagcc actcctcggc 2760

aaggcacttt ccagggtcaa gcgggccgag aagaaatgga gggacaagta cgagaaactc 2820

cagctcgaaa caaagcgggt gtacacggag gcaaaggaat ccgtggacgc cctgttcgtg 2880

gactctcagt acgacaagct ccaggcgaac acaaacattg gcatcatcca cggtgcggac 2940

aagcaagtgc acaggatacg ggagccttac ctctcggagc tgccggtgat tccctcgatc 3000

aacgcggcga tcttcgagga actggagggc cacatcttca aggcgtattc tctgtacgac 3060

gcgcgtaacg tcatcaagaa cggcgacttc aacaatgggc tgtcctgctg gaacgttaaa 3120

ggccacgtcg atgtccagca gaaccaccat aggtcagtcc tggtgctgag cgagtgggag 3180

gcggaggtgt cccagaaggt gcgcgtgtgc ccggatcgcg gctacatctt gagggtgaca 3240

gcctacaagg agggctacgg cgagggctgt gtcacgatcc atgagttcga ggacaacacg 3300

gatgtcctga aattccgtaa cttcgtcgag gaggaggtct atcccaacaa caccgtgacc 3360

tgcaacgact acacgaccaa tcagtcggct gagggcagta ccgatgcctg caacagctac 3420

aaccgtggtt acgaagatgg atacgagaac cgctacgagc ccaatccttc ggctcccgtg 3480

aattacactc ccacgtacga ggagggcatg tacactgaca ctcagggcta caaccattgc 3540

gtcagcgacc gtggctaccg caaccacacg ccgctcccag cgggctacgt gacgctggag 3600

ctggaatact ttcccgagac agaacaagtg tggatagaga tcggcgagac cgagggcaca 3660

ttcatcgtgg gctctgtgga attgctcctc atggaggagt aa 3702

<210> 43

<211> 1200

<212> PRT

<213> 人工的

<220>

<223> 嵌合蛋白质变体TIC868_14的氨基酸序列。

<400> 43

Met Thr Ser Asn Arg Lys Asn Glu Asn Glu Ile Ile Asn Ala Leu Ser

1 5 10 15

Ile Pro Ala Val Ser Asn His Ser Ala Gln Met Asn Leu Ser Thr Asp

20 25 30

Ala Arg Ile Glu Asp Ser Leu Cys Ile Ala Glu Gly Asn Asn Ile Asp

35 40 45

Pro Phe Val Ser Ala Ser Thr Val Gln Thr Gly Ile Asn Ile Ala Gly

50 55 60

Arg Ile Leu Gly Val Leu Gly Val Pro Phe Ala Gly Gln Ile Ala Ser

65 70 75 80

Phe Tyr Ser Phe Leu Val Gly Glu Leu Trp Pro Arg Gly Arg Asp Pro

85 90 95

Trp Glu Ile Phe Leu Glu His Val Glu Gln Leu Ile Arg Gln Gln Val

100 105 110

Thr Glu Asn Thr Arg Asp Thr Ala Leu Ala Arg Leu Gln Gly Leu Gly

115 120 125

Asn Ser Phe Arg Ala Tyr Gln Gln Ser Leu Glu Asp Trp Leu Glu Asn

130 135 140

Arg Asp Asp Ala Arg Thr Arg Ser Val Leu Tyr Thr Gln Tyr Ile Ala

145 150 155 160

Leu Glu Leu Asp Phe Leu Asn Ala Met Pro Leu Phe Ala Ile Arg Asn

165 170 175

Gln Glu Val Pro Leu Leu Met Val Tyr Ala Gln Ala Ala Asn Leu His

180 185 190

Leu Leu Leu Leu Arg Asp Ala Ser Leu Phe Gly Ser Glu Phe Gly Leu

195 200 205

Thr Ser Gln Glu Ile Gln Arg Tyr Tyr Glu Arg Gln Val Glu Lys Thr

210 215 220

Arg Glu Tyr Ser Asp Tyr Cys Ala Arg Trp Tyr Asn Thr Gly Leu Asn

225 230 235 240

Asn Leu Arg Gly Thr Asn Ala Glu Ser Trp Leu Arg Tyr Asn Gln Phe

245 250 255

Arg Arg Asp Leu Thr Leu Gly Val Leu Asp Leu Val Ala Leu Phe Pro

260 265 270

Ser Tyr Asp Thr Arg Val Tyr Pro Met Asn Thr Ser Ala Gln Leu Thr

275 280 285

Arg Glu Ile Tyr Thr Asp Pro Ile Gly Arg Thr Asn Ala Pro Ser Gly

290 295 300

Phe Ala Ser Thr Asn Trp Phe Asn Asn Asn Ala Pro Ser Phe Ser Ala

305 310 315 320

Ile Glu Ala Ala Val Ile Arg Pro Pro His Leu Leu Asp Phe Pro Glu

325 330 335

Gln Leu Thr Ile Phe Ser Val Leu Ser Arg Trp Ser Asn Thr Gln Tyr

340 345 350

Met Asn Tyr Trp Val Gly His Arg Leu Glu Ser Arg Thr Ile Arg Gly

355 360 365

Ser Leu Ser Thr Ser Thr His Gly Asn Thr Asn Thr Ser Ile Asn Pro

370 375 380

Val Thr Leu Gln Phe Thr Ser Arg Asp Val Tyr Arg Thr Glu Ser Phe

385 390 395 400

Ala Gly Ile Asn Ile Leu Leu Thr Thr Pro Val Asn Gly Val Pro Trp

405 410 415

Ala Arg Phe Asn Trp Arg Asn Pro Leu Asn Ser Leu Arg Gly Ser Leu

420 425 430

Leu Tyr Thr Ile Gly Tyr Thr Gly Val Gly Thr Gln Leu Phe Asp Ser

435 440 445

Glu Thr Glu Leu Pro Pro Glu Thr Thr Glu Arg Pro Asn Tyr Glu Ser

450 455 460

Tyr Ser His Arg Leu Ser Asn Ile Arg Leu Ile Ser Gly Asn Thr Leu

465 470 475 480

Arg Ala Pro Val Tyr Ser Trp Thr His Arg Ser Ala Asp Arg Thr Asn

485 490 495

Thr Ile Ser Ser Asp Ser Ile Asn Gln Ile Pro Leu Val Lys Gly Phe

500 505 510

Arg Val Trp Gly Gly Thr Ser Val Ile Thr Gly Pro Gly Phe Thr Gly

515 520 525

Gly Asp Ile Leu Arg Arg Asn Thr Phe Gly Asp Phe Val Ser Leu Gln

530 535 540

Val Asn Ile Asn Ser Pro Ile Thr Gln Arg Tyr Arg Leu Arg Phe Arg

545 550 555 560

Tyr Ala Ser Ser Arg Asp Ala Arg Val Ile Val Leu Thr Gly Ala Ala

565 570 575

Ser Thr Gly Val Gly Gly Gln Val Ser Val Asn Met Pro Leu Gln Lys

580 585 590

Thr Met Glu Ile Gly Glu Asn Leu Thr Ser Arg Thr Phe Arg Tyr Thr

595 600 605

Asp Phe Ser Asn Pro Phe Ser Phe Arg Ala Asn Pro Asp Ile Ile Gly

610 615 620

Ile Ser Glu Gln Pro Leu Phe Gly Ala Gly Ser Ile Ser Ser Gly Glu

625 630 635 640

Leu Tyr Ile Asp Lys Ile Glu Ile Ile Leu Ala Asp Ala Thr Thr Ala

645 650 655

Thr Phe Glu Ala Glu Ser Asp Leu Glu Arg Ala Arg Lys Ala Val Asn

660 665 670

Ala Leu Phe Thr Ser Thr Asn Pro Arg Gly Leu Lys Thr Asp Val Thr

675 680 685

Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Glu Cys Leu Ser Asp

690 695 700

Glu Phe Cys Leu Asp Lys Lys Arg Glu Leu Leu Glu Glu Val Lys Tyr

705 710 715 720

Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Thr Phe

725 730 735

Thr Ser Ile Ser Gly Gln Thr Asp Arg Gly Trp Ile Gly Ser Thr Gly

740 745 750

Ile Ser Ile Gln Gly Gly Asp Asp Ile Phe Lys Glu Asn Tyr Val Arg

755 760 765

Leu Pro Gly Thr Val Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln Lys

770 775 780

Ile Asp Glu Ser Gln Leu Lys Ser Tyr Thr Arg Tyr Gln Leu Arg Gly

785 790 795 800

Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr Asn

805 810 815

Ala Lys His Glu Thr Leu Ser Val Pro Gly Thr Glu Ser Pro Trp Pro

820 825 830

Ser Ser Gly Val Tyr Pro Ser Gly Arg Cys Gly Glu Pro Asn Arg Cys

835 840 845

Ala Pro Arg Ile Glu Trp Asn Pro Asp Leu Asp Cys Ser Cys Arg Tyr

850 855 860

Gly Glu Lys Cys Val His His Ser His His Phe Ser Leu Asp Ile Asp

865 870 875 880

Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Val Ile Phe

885 890 895

Lys Ile Lys Thr Gln Asp Gly His Ala Lys Leu Gly Asn Leu Glu Phe

900 905 910

Ile Glu Glu Lys Pro Leu Leu Gly Lys Ala Leu Ser Arg Val Lys Arg

915 920 925

Ala Glu Lys Lys Trp Arg Asp Lys Tyr Glu Lys Leu Gln Leu Glu Thr

930 935 940

Lys Arg Val Tyr Thr Glu Ala Lys Glu Ser Val Asp Ala Leu Phe Val

945 950 955 960

Asp Ser Gln Tyr Asp Lys Leu Gln Ala Asn Thr Asn Ile Gly Ile Ile

965 970 975

His Gly Ala Asp Lys Gln Val His Arg Ile Arg Glu Pro Tyr Leu Ser

980 985 990

Glu Leu Pro Val Ile Pro Ser Ile Asn Ala Ala Ile Phe Glu Glu Leu

995 1000 1005

Glu Gly His Ile Phe Lys Ala Tyr Ser Leu Tyr Asp Ala Arg Asn

1010 1015 1020

Val Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Ser Cys Trp Asn

1025 1030 1035

Val Lys Gly His Val Asp Val Gln Gln Asn His His Arg Ser Val

1040 1045 1050

Leu Val Leu Ser Glu Trp Glu Ala Glu Val Ser Gln Lys Val Arg

1055 1060 1065

Val Cys Pro Asp Arg Gly Tyr Ile Leu Arg Val Thr Ala Tyr Lys

1070 1075 1080

Glu Gly Tyr Gly Glu Gly Cys Val Thr Ile His Glu Phe Glu Asp

1085 1090 1095

Asn Thr Asp Val Leu Lys Phe Arg Asn Phe Val Glu Glu Glu Val

1100 1105 1110

Tyr Pro Asn Asn Thr Val Thr Cys Asn Asp Tyr Thr Thr Asn Gln

1115 1120 1125

Ser Ala Glu Gly Ser Thr Asp Ala Cys Asn Ser Tyr Asn Arg Gly

1130 1135 1140

Tyr Glu Asp Gly Tyr Glu Asn Arg Tyr Glu Pro Asn Pro Ser Ala

1145 1150 1155

Pro Val Asn Tyr Thr Pro Thr Tyr Glu Glu Gly Met Tyr Thr Asp

1160 1165 1170

Thr Gln Gly Tyr Asn His Cys Val Ser Asp Arg Gly Tyr Arg Asn

1175 1180 1185

His Thr Pro Leu Pro Ala Gly Tyr Val Thr Leu Glu

1190 1195 1200

<210> 44

<211> 3687

<212> DNA

<213> 人工的

<220>

<223> 设计用于在植物细胞中表达的编码TIC868_15的合成核苷酸序列。

<400> 44

atgacgagca accggaagaa cgagaacgag atcatcaacg ccctctcgat ccctgctgtt 60

tcaaaccact ccgcgcagat gaacctgtcc accgacgcgc gcatcgagga ctccctctgc 120

atagccgagg gcaacaacat cgacccattc gtgtcggcca gcacggttca gaccggcatc 180

aacatcgcgg gccgtatcct cggcgtcctc ggtgtcccat tcgccggtca gatcgcgtcc 240

ttctactcgt tccttgtggg cgagctgtgg cctcgcggtc gtgacccgtg ggagatcttc 300

ctggagcatg tggagcagtt gatccggcag caagtcacgg agaacacccg cgatactgct 360

ctggccaggc tacagggcct gggaaactcc tttcgggcat accagcagtc actggaggac 420

tggttggaga acagggatga cgcgcgaaca cgctcggtac tctacaccca gtacatcgct 480

ctcgaactcg acttcctgaa cgctatgccg ctgttcgcca tcaggaacca ggaagttcca 540

ctccttatgg tgtacgccca ggccgccaac ttacatctgc tcctgctgcg ggacgccagc 600

ctgttcggct ccgagttcgg actcacatct caagaaatcc agcgttacta cgagcgccaa 660

gtggagaaga cccgtgagta cagtgactac tgcgctcgat ggtacaacac agggctcaac 720

aacctgcgcg gcaccaacgc tgagtcatgg ctccgttaca accagttccg ccgcgacttg 780

actttgggtg tcctagacct ggtggcgcta ttcccgtctt acgacacacg ggtgtaccca 840

atgaacacta gcgcgcaact cacgcgggag atctacacag acccaatcgg ccggacgaac 900

gcaccctccg gtttcgcatc cacgaattgg ttcaacaaca acgcaccctc cttctcggca 960

atcgaggccg ccgtcatccg ccctcctcac ctgctcgact ttcccgagca gctcacgatc 1020

ttctccgtgc tctcacgctg gtccaacaca cagtacatga actactgggt cgggcaccga 1080

ttggagagta ggacgatccg tggcagcttg agcaccagta cccacggcaa caccaacacc 1140

tccatcaacc cagttacgct acagttcacg agccgcgacg tttaccggac tgagtcgttc 1200

gcgggcatta acatccttct gacaacgccc gtcaacggcg tcccgtgggc ccggttcaac 1260

tggcgtaacc cgttgaactc cctgcgcggg tcattgctct acaccatcgg gtacacgggc 1320

gtcggcaccc agctcttcga cagtgaaact gagctgccgc ccgagaccac ggaacgcccg 1380

aactacgagt cctacagcca ccgcctgtcc aacatccggc tcatctctgg caacacgctg 1440

cgtgcgccgg tgtactcctg gacacaccgc agcgccgacc ggaccaacac gatctcttcc 1500

gactccatta accagatccc gctcgtgaag ggcttccgtg tgtggggtgg cacgagcgtc 1560

atcaccggtc cgggcttcac cggtggagac atactgcggc gcaacacttt cggcgacttc 1620

gtttcgttgc aagtgaacat caactcgccg atcacccagc gttaccgtct gaggttccgc 1680

tacgcttcaa gccgcgacgc gagggtcatt gtcctgaccg gagccgcgtc cacaggcgtg 1740

ggaggccaag tctcagtcaa catgcctctc cagaagacga tggagatagg cgagaacttg 1800

actagccgaa ccttccggta cactgatttc tcgaaccctt tctcattcag agcgaaccct 1860

gacatcattg ggatctccga gcaaccgctg ttcggtgctg gctccatcag ctctggcgaa 1920

ctgtacatcg acaagattga gatcatcctg gcggatgcga cggatgctac ctttgaagca 1980

gagtccgact tggaacgtgc acagaaggca gtgaacgcac tcttcacctc aagcaaccag 2040

atcggattga agacagatgt gacagattac cacatcgacc aagtgagcaa cttggtggat 2100

tgcttgtcag atgagttctg cttggatgag aagcgtgaac tctccgagaa ggtgaagcac 2160

gcaaagcgtc tctcagatga acgtaatctc cttcaagacc ctaactttcg tggtatcaat 2220

cgtcagccag atcgtggatg gcgtggatca acagacatca ccatccaggg aggcgatgat 2280

gtgttcaagg agaactacgt gaccctccca ggaaccgtgg atgaatgcta cccaacctac 2340

ctctaccaga agatcgacga gtcaaagctc aaggcttaca cccgttatga actccgtggc 2400

tacatcgaag atagccagga tctcgaaatc tatctcatcc gttacaatgc taagcacgaa 2460

atcgtgaatg tgccaggaac cggctcactc tggccactct cagcacagtc accaatcggc 2520

aagtgcggcg aacccaatcg ctgcgctcct catctcgaat ggaatcccga tctcgactgc 2580

tcctgccgag acggcgagaa gtgtgcacat cactcacacc acttcaccct cgacatcgac 2640

gtgggctgca ccgacctcaa tgaagacctg ggcgtgtggg tgatcttcaa gatcaagacc 2700

caggacggcc acgcacgact gggcaatctg gagtttctgg aggagaagcc actgcttggc 2760

gaggcactgg cacgagtgaa acgagccgag aagaaatggc gagacaaacg tgagaagctg 2820

caactggaga ccaacatcgt gtacaaagag gccaaagagt cagttgacgc cctgtttgtc 2880

aatagccagt atgaccgact gcaagttgac accaacatcg ccatgatcca cgctgcggac 2940

aagcgcgtcc accgcatccg cgaggcttat ctgcccgagc tgagcgtcat tcccggcgtc 3000

aatgccgcga tcttcgagga gttagagggc cgcatcttca ccgcctacag cctctatgac 3060

gcccgcaatg tcattaagaa tggcgacttc aacaatggct tactatgctg gaatgtcaaa 3120

gggcacgttg acgtcgagga gcagaacaat caccgcagcg tcttagtcat acccgagtgg 3180

gaggccgaag tcagccagga agtccgcgtc tgtccagggc gcgggtacat cctgcgggtc 3240

accgcctaca aagagggata cggcgagggt tgtgtcacca tacacgagat agaggacaat 3300

accgacgaac tcaagttcag caattgtgtc gaggaggaag tctatcccaa caataccgta 3360

acctgcaaca actacaccgg aacccaggag gagtatgaag ggacgtacac ctcgcggaac 3420

cagggctatg acgaagccta tgggaacaac ccgtcggtgc ctgctgacta tgcgtcggtc 3480

tatgaggaga aatcgtacac ggacgggcgg cgggagaatc cgtgtgagtc gaatcgcggg 3540

tatggtgact acacgccgct accggcgggc tatgtaacga aagacctgga atacttcccg 3600

gagacggaca aagtatggat agagataggc gagacggagg gaacgttcat cgtggactcg 3660

gtagagctgc tgctcatgga ggagtga 3687

<210> 45

<211> 1228

<212> PRT

<213> 人工的

<220>

<223> 嵌合蛋白质变体TIC868_15的氨基酸序列。

<400> 45

Met Thr Ser Asn Arg Lys Asn Glu Asn Glu Ile Ile Asn Ala Leu Ser

1 5 10 15

Ile Pro Ala Val Ser Asn His Ser Ala Gln Met Asn Leu Ser Thr Asp

20 25 30

Ala Arg Ile Glu Asp Ser Leu Cys Ile Ala Glu Gly Asn Asn Ile Asp

35 40 45

Pro Phe Val Ser Ala Ser Thr Val Gln Thr Gly Ile Asn Ile Ala Gly

50 55 60

Arg Ile Leu Gly Val Leu Gly Val Pro Phe Ala Gly Gln Ile Ala Ser

65 70 75 80

Phe Tyr Ser Phe Leu Val Gly Glu Leu Trp Pro Arg Gly Arg Asp Pro

85 90 95

Trp Glu Ile Phe Leu Glu His Val Glu Gln Leu Ile Arg Gln Gln Val

100 105 110

Thr Glu Asn Thr Arg Asp Thr Ala Leu Ala Arg Leu Gln Gly Leu Gly

115 120 125

Asn Ser Phe Arg Ala Tyr Gln Gln Ser Leu Glu Asp Trp Leu Glu Asn

130 135 140

Arg Asp Asp Ala Arg Thr Arg Ser Val Leu Tyr Thr Gln Tyr Ile Ala

145 150 155 160

Leu Glu Leu Asp Phe Leu Asn Ala Met Pro Leu Phe Ala Ile Arg Asn

165 170 175

Gln Glu Val Pro Leu Leu Met Val Tyr Ala Gln Ala Ala Asn Leu His

180 185 190

Leu Leu Leu Leu Arg Asp Ala Ser Leu Phe Gly Ser Glu Phe Gly Leu

195 200 205

Thr Ser Gln Glu Ile Gln Arg Tyr Tyr Glu Arg Gln Val Glu Lys Thr

210 215 220

Arg Glu Tyr Ser Asp Tyr Cys Ala Arg Trp Tyr Asn Thr Gly Leu Asn

225 230 235 240

Asn Leu Arg Gly Thr Asn Ala Glu Ser Trp Leu Arg Tyr Asn Gln Phe

245 250 255

Arg Arg Asp Leu Thr Leu Gly Val Leu Asp Leu Val Ala Leu Phe Pro

260 265 270

Ser Tyr Asp Thr Arg Val Tyr Pro Met Asn Thr Ser Ala Gln Leu Thr

275 280 285

Arg Glu Ile Tyr Thr Asp Pro Ile Gly Arg Thr Asn Ala Pro Ser Gly

290 295 300

Phe Ala Ser Thr Asn Trp Phe Asn Asn Asn Ala Pro Ser Phe Ser Ala

305 310 315 320

Ile Glu Ala Ala Val Ile Arg Pro Pro His Leu Leu Asp Phe Pro Glu

325 330 335

Gln Leu Thr Ile Phe Ser Val Leu Ser Arg Trp Ser Asn Thr Gln Tyr

340 345 350

Met Asn Tyr Trp Val Gly His Arg Leu Glu Ser Arg Thr Ile Arg Gly

355 360 365

Ser Leu Ser Thr Ser Thr His Gly Asn Thr Asn Thr Ser Ile Asn Pro

370 375 380

Val Thr Leu Gln Phe Thr Ser Arg Asp Val Tyr Arg Thr Glu Ser Phe

385 390 395 400

Ala Gly Ile Asn Ile Leu Leu Thr Thr Pro Val Asn Gly Val Pro Trp

405 410 415

Ala Arg Phe Asn Trp Arg Asn Pro Leu Asn Ser Leu Arg Gly Ser Leu

420 425 430

Leu Tyr Thr Ile Gly Tyr Thr Gly Val Gly Thr Gln Leu Phe Asp Ser

435 440 445

Glu Thr Glu Leu Pro Pro Glu Thr Thr Glu Arg Pro Asn Tyr Glu Ser

450 455 460

Tyr Ser His Arg Leu Ser Asn Ile Arg Leu Ile Ser Gly Asn Thr Leu

465 470 475 480

Arg Ala Pro Val Tyr Ser Trp Thr His Arg Ser Ala Asp Arg Thr Asn

485 490 495

Thr Ile Ser Ser Asp Ser Ile Asn Gln Ile Pro Leu Val Lys Gly Phe

500 505 510

Arg Val Trp Gly Gly Thr Ser Val Ile Thr Gly Pro Gly Phe Thr Gly

515 520 525

Gly Asp Ile Leu Arg Arg Asn Thr Phe Gly Asp Phe Val Ser Leu Gln

530 535 540

Val Asn Ile Asn Ser Pro Ile Thr Gln Arg Tyr Arg Leu Arg Phe Arg

545 550 555 560

Tyr Ala Ser Ser Arg Asp Ala Arg Val Ile Val Leu Thr Gly Ala Ala

565 570 575

Ser Thr Gly Val Gly Gly Gln Val Ser Val Asn Met Pro Leu Gln Lys

580 585 590

Thr Met Glu Ile Gly Glu Asn Leu Thr Ser Arg Thr Phe Arg Tyr Thr

595 600 605

Asp Phe Ser Asn Pro Phe Ser Phe Arg Ala Asn Pro Asp Ile Ile Gly

610 615 620

Ile Ser Glu Gln Pro Leu Phe Gly Ala Gly Ser Ile Ser Ser Gly Glu

625 630 635 640

Leu Tyr Ile Asp Lys Ile Glu Ile Ile Leu Ala Asp Ala Thr Asp Ala

645 650 655

Thr Phe Glu Ala Glu Ser Asp Leu Glu Arg Ala Gln Lys Ala Val Asn

660 665 670

Ala Leu Phe Thr Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp Val Thr

675 680 685

Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val Asp Cys Leu Ser Asp

690 695 700

Glu Phe Cys Leu Asp Glu Lys Arg Glu Leu Ser Glu Lys Val Lys His

705 710 715 720

Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn Phe

725 730 735

Arg Gly Ile Asn Arg Gln Pro Asp Arg Gly Trp Arg Gly Ser Thr Asp

740 745 750

Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val Thr

755 760 765

Leu Pro Gly Thr Val Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln Lys

770 775 780

Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Glu Leu Arg Gly

785 790 795 800

Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr Asn

805 810 815

Ala Lys His Glu Ile Val Asn Val Pro Gly Thr Gly Ser Leu Trp Pro

820 825 830

Leu Ser Ala Gln Ser Pro Ile Gly Lys Cys Gly Glu Pro Asn Arg Cys

835 840 845

Ala Pro His Leu Glu Trp Asn Pro Asp Leu Asp Cys Ser Cys Arg Asp

850 855 860

Gly Glu Lys Cys Ala His His Ser His His Phe Thr Leu Asp Ile Asp

865 870 875 880

Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp Val Ile Phe

885 890 895

Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn Leu Glu Phe

900 905 910

Leu Glu Glu Lys Pro Leu Leu Gly Glu Ala Leu Ala Arg Val Lys Arg

915 920 925

Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Lys Leu Gln Leu Glu Thr

930 935 940

Asn Ile Val Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala Leu Phe Val

945 950 955 960

Asn Ser Gln Tyr Asp Arg Leu Gln Val Asp Thr Asn Ile Ala Met Ile

965 970 975

His Ala Ala Asp Lys Arg Val His Arg Ile Arg Glu Ala Tyr Leu Pro

980 985 990

Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile Phe Glu Glu Leu

995 1000 1005

Glu Gly Arg Ile Phe Thr Ala Tyr Ser Leu Tyr Asp Ala Arg Asn

1010 1015 1020

Val Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Leu Cys Trp Asn

1025 1030 1035

Val Lys Gly His Val Asp Val Glu Glu Gln Asn Asn His Arg Ser

1040 1045 1050

Val Leu Val Ile Pro Glu Trp Glu Ala Glu Val Ser Gln Glu Val

1055 1060 1065

Arg Val Cys Pro Gly Arg Gly Tyr Ile Leu Arg Val Thr Ala Tyr

1070 1075 1080

Lys Glu Gly Tyr Gly Glu Gly Cys Val Thr Ile His Glu Ile Glu

1085 1090 1095

Asp Asn Thr Asp Glu Leu Lys Phe Ser Asn Cys Val Glu Glu Glu

1100 1105 1110

Val Tyr Pro Asn Asn Thr Val Thr Cys Asn Asn Tyr Thr Gly Thr

1115 1120 1125

Gln Glu Glu Tyr Glu Gly Thr Tyr Thr Ser Arg Asn Gln Gly Tyr

1130 1135 1140

Asp Glu Ala Tyr Gly Asn Asn Pro Ser Val Pro Ala Asp Tyr Ala

1145 1150 1155

Ser Val Tyr Glu Glu Lys Ser Tyr Thr Asp Gly Arg Arg Glu Asn

1160 1165 1170

Pro Cys Glu Ser Asn Arg Gly Tyr Gly Asp Tyr Thr Pro Leu Pro

1175 1180 1185

Ala Gly Tyr Val Thr Lys Asp Leu Glu Tyr Phe Pro Glu Thr Asp

1190 1195 1200

Lys Val Trp Ile Glu Ile Gly Glu Thr Glu Gly Thr Phe Ile Val

1205 1210 1215

Asp Ser Val Glu Leu Leu Leu Met Glu Glu

1220 1225

<210> 46

<211> 3600

<212> DNA

<213> 人工的

<220>

<223> 设计用于在植物细胞中表达的编码TIC868_29的合成核苷酸序列。

<400> 46

atgacgagca accggaagaa cgagaacgag atcatcaacg ccctctcgat ccctgctgtt 60

tcaaaccact ccgcgcagat gaacctgtcc accgacgcgc gcatcgagga ctccctctgc 120

atagccgagg gcaacaacat cgacccattc gtgtcggcca gcacggttca gaccggcatc 180

aacatcgcgg gccgtatcct cggcgtcctc ggtgtcccat tcgccggtca gatcgcgtcc 240

ttctactcgt tccttgtggg cgagctgtgg cctcgcggtc gtgacccgtg ggagatcttc 300

ctggagcatg tggagcagtt gatccggcag caagtcacgg agaacacccg cgatactgct 360

ctggccaggc tacagggcct gggaaactcc tttcgggcat accagtactc actggaggac 420

tggttggaga acagggatga cgcgcgaaca cgctcggtac tctacaccca gtacatcgct 480

ctcgaactcg acttcctgaa cgctatgccg ctgttcgcca tcaggaacca ggaagttcca 540

ctccttatgg tgtacgccca ggccgccaac ttacatctgc tcctgctgcg ggacgccagc 600

ctgttcggct ccgagttcgg actcacatct caagaaatcc agcgttacta cgagcgccaa 660

gtggagaaga cccgtgagta cagtgactac tgcgctcgat ggtacaacac agggctcaac 720

aacctgcgcg gcaccaacgc tgagtcatgg ctccgttaca accagttccg ccgcgacttg 780

actttgggtg tcctagacct ggtggcgcta ttcccgtctt acgacacacg ggtgtaccca 840

atgaacacta gcgcgcaact cacgcgggag atctacacag acccaatcgg ccggacgaac 900

gcaccctccg gtttcgcatc cacgaattgg ttcaacaaca acgcaccctc cttctcggca 960

atcgaggccg ccgtcatccg ccctcctcac ctgctcgact ttcccgagca gctcacgatc 1020

ttctcccagc tctcacgctg gtcccacaca cagtacatga actactgggt cgggcaccga 1080

ttggagagta ggacgatccg tggcagcttg agcaccagta cccacggcaa caccaacacc 1140

tccatcaacc cagttacgct acagttcacg agccgcgacg tttaccggac tgagtcgttc 1200

gcgggcatta acatccttct gacaacgccc gtcaacggcg tcccgtgggc ccggttcaac 1260

tggcgtaacc cgttgaactc cctgcgcggg tcattgctct acaccatcgg gtacacgggc 1320

gtcggcaccc agctcttcga cagtgaaact gagctgccgc ccgagaccac ggaacgcccg 1380

aactacgagt cctacagcca ccgcctgtcc aacatccggc tcatctctgg caacacgctg 1440

cgtgcgccgg tgtactcctg gacacaccgc agcgccgacc ggaccaacac gatctcttcc 1500

gactccatta accagatccc gctcgtgaag ggcttccgtg tgtggggtgg cacgagcgtc 1560

atcaccggtc cgggcttcac cggtggagac atactgcggc gcaacacttt cggcgacttc 1620

gtttcgttgc aagtgaacat caactcgccg atcacccagc gttaccgtct gaggttccgc 1680

tacgcttcaa gccgcgacgc gagggtcatt gtcctgaccg gagccgcgtc cacaggcgtg 1740

ggaggccaag tctcagtcaa catgcctctc cagaagacga tggagatagg cgagaacttg 1800

actagccgaa ccttccggta cactgatttc tcgaaccctt tctcattcag agcgaaccct 1860

gacatcattg ggatctccga gcaaccgctg ttcggtgctg gctccatcag ctctggcgaa 1920

ctgtacatcg acaagattga gatcatcctg gcggatgcga cgttcgaggc cgagtctgac 1980

ctggagcggg ctcagaaggc tgtcaacgaa ctgttcacca gcagcaacca gattgggctc 2040

aagaccgacg tcacggacta tcacattgac caagtgtcca accttgtgga gtgcctgtcc 2100

gacgagttct gcctcgacga gaagaaggag ctgtccgaga aggtcaaaca cgcgaagcgt 2160

ctgagtgacg agcggaattt gctccaggac ccgaacttcc gtggcatcaa ccgccagctc 2220

gaccgtggtt ggcgcgggag tacagacatc accatccagg gaggcgacga tgtgttcaag 2280

gagaactatg tgacgctgct cgggactttc gacgaatgct acccgacgta tctctaccag 2340

aagatagacg agagtaaatt gaaggcgtac acccgctacc agcttcgcgg gtacatcgag 2400

gatagtcagg acctggaaat ctacctgatc cgatacaacg ccaagcacga gacagtgaac 2460

gtgccaggca cgggctcact ttggccattg agcgctccct ctccaatcgg aaagtgcgct 2520

caccactcgc accacttctc tctggacatc gacgtgggct gcaccgacct caacgaggac 2580

ctgggtgtct gggttatctt caagattaag acccaggacg gacatgcccg cctcggcaac 2640

ctggagttcc ttgaggagaa gcctctcgtg ggcgaggccc tcgctcgtgt gaagcgcgcc 2700

gagaagaaat ggcgagacaa gcgggagaag ctggagtggg agaccaacat cgtgtacaag 2760

gaggccaagg agtcagtgga cgcactcttc gtcaacagcc agtacgaccg cctccaggct 2820

gacaccaaca tcgccatgat ccacgcggct gacaagcggg tccacagcat ccgtgaggcg 2880

tacctgcccg agctgtcagt gatccctggt gtgaacgcgg cgatcttcga ggaactggag 2940

ggccgcatct tcacagcatt cagcctgtac gatgccagga atgttattaa gaacggtgac 3000

ttcaacaacg ggctgagttg ctggaacgtc aagggccatg tggacgtcga ggagcagaac 3060

aaccaccggt ccgtgctggt cgtgccggag tgggaggcag aggtgagcca ggaggtccgc 3120

gtctgccctg gtcgcggcta catcctccgt gtgactgcgt acaaggaagg ctacggtgaa 3180

ggctgcgtga ctatccacga gatcgagaac aacaccgacg agctcaagtt ctcgaactgt 3240

gtggaggagg aggtgtaccc gaacaacacc gttacttgca acgactacac tgccacgcaa 3300

gaggagtacg agggcactta cacttcccgg aatcgcggct atgatggcgc gtacgagtcc 3360

aacagcagcg tgcctgcgga ttatgcgtcc gcttacgagg agaaggcgta caccgacgga 3420

cggagggaca acccttgcga gtccaaccgt ggctacggtg actacactcc gctgcccgcc 3480

gggtacgtca ccaaggagct ggagtacttc ccggagaccg acaaagtctg gatcgagatc 3540

ggcgagacgg agggcacttt catcgtggac tcggtcgagc tgctactgat ggaggagtga 3600

<210> 47

<211> 1199

<212> PRT

<213> 人工的

<220>

<223> 嵌合蛋白质变体TIC868_29的氨基酸序列。

<400> 47

Met Thr Ser Asn Arg Lys Asn Glu Asn Glu Ile Ile Asn Ala Leu Ser

1 5 10 15

Ile Pro Ala Val Ser Asn His Ser Ala Gln Met Asn Leu Ser Thr Asp

20 25 30

Ala Arg Ile Glu Asp Ser Leu Cys Ile Ala Glu Gly Asn Asn Ile Asp

35 40 45

Pro Phe Val Ser Ala Ser Thr Val Gln Thr Gly Ile Asn Ile Ala Gly

50 55 60

Arg Ile Leu Gly Val Leu Gly Val Pro Phe Ala Gly Gln Ile Ala Ser

65 70 75 80

Phe Tyr Ser Phe Leu Val Gly Glu Leu Trp Pro Arg Gly Arg Asp Pro

85 90 95

Trp Glu Ile Phe Leu Glu His Val Glu Gln Leu Ile Arg Gln Gln Val

100 105 110

Thr Glu Asn Thr Arg Asp Thr Ala Leu Ala Arg Leu Gln Gly Leu Gly

115 120 125

Asn Ser Phe Arg Ala Tyr Gln Tyr Ser Leu Glu Asp Trp Leu Glu Asn

130 135 140

Arg Asp Asp Ala Arg Thr Arg Ser Val Leu Tyr Thr Gln Tyr Ile Ala

145 150 155 160

Leu Glu Leu Asp Phe Leu Asn Ala Met Pro Leu Phe Ala Ile Arg Asn

165 170 175

Gln Glu Val Pro Leu Leu Met Val Tyr Ala Gln Ala Ala Asn Leu His

180 185 190

Leu Leu Leu Leu Arg Asp Ala Ser Leu Phe Gly Ser Glu Phe Gly Leu

195 200 205

Thr Ser Gln Glu Ile Gln Arg Tyr Tyr Glu Arg Gln Val Glu Lys Thr

210 215 220

Arg Glu Tyr Ser Asp Tyr Cys Ala Arg Trp Tyr Asn Thr Gly Leu Asn

225 230 235 240

Asn Leu Arg Gly Thr Asn Ala Glu Ser Trp Leu Arg Tyr Asn Gln Phe

245 250 255

Arg Arg Asp Leu Thr Leu Gly Val Leu Asp Leu Val Ala Leu Phe Pro

260 265 270

Ser Tyr Asp Thr Arg Val Tyr Pro Met Asn Thr Ser Ala Gln Leu Thr

275 280 285

Arg Glu Ile Tyr Thr Asp Pro Ile Gly Arg Thr Asn Ala Pro Ser Gly

290 295 300

Phe Ala Ser Thr Asn Trp Phe Asn Asn Asn Ala Pro Ser Phe Ser Ala

305 310 315 320

Ile Glu Ala Ala Val Ile Arg Pro Pro His Leu Leu Asp Phe Pro Glu

325 330 335

Gln Leu Thr Ile Phe Ser Gln Leu Ser Arg Trp Ser His Thr Gln Tyr

340 345 350

Met Asn Tyr Trp Val Gly His Arg Leu Glu Ser Arg Thr Ile Arg Gly

355 360 365

Ser Leu Ser Thr Ser Thr His Gly Asn Thr Asn Thr Ser Ile Asn Pro

370 375 380

Val Thr Leu Gln Phe Thr Ser Arg Asp Val Tyr Arg Thr Glu Ser Phe

385 390 395 400

Ala Gly Ile Asn Ile Leu Leu Thr Thr Pro Val Asn Gly Val Pro Trp

405 410 415

Ala Arg Phe Asn Trp Arg Asn Pro Leu Asn Ser Leu Arg Gly Ser Leu

420 425 430

Leu Tyr Thr Ile Gly Tyr Thr Gly Val Gly Thr Gln Leu Phe Asp Ser

435 440 445

Glu Thr Glu Leu Pro Pro Glu Thr Thr Glu Arg Pro Asn Tyr Glu Ser

450 455 460

Tyr Ser His Arg Leu Ser Asn Ile Arg Leu Ile Ser Gly Asn Thr Leu

465 470 475 480

Arg Ala Pro Val Tyr Ser Trp Thr His Arg Ser Ala Asp Arg Thr Asn

485 490 495

Thr Ile Ser Ser Asp Ser Ile Asn Gln Ile Pro Leu Val Lys Gly Phe

500 505 510

Arg Val Trp Gly Gly Thr Ser Val Ile Thr Gly Pro Gly Phe Thr Gly

515 520 525

Gly Asp Ile Leu Arg Arg Asn Thr Phe Gly Asp Phe Val Ser Leu Gln

530 535 540

Val Asn Ile Asn Ser Pro Ile Thr Gln Arg Tyr Arg Leu Arg Phe Arg

545 550 555 560

Tyr Ala Ser Ser Arg Asp Ala Arg Val Ile Val Leu Thr Gly Ala Ala

565 570 575

Ser Thr Gly Val Gly Gly Gln Val Ser Val Asn Met Pro Leu Gln Lys

580 585 590

Thr Met Glu Ile Gly Glu Asn Leu Thr Ser Arg Thr Phe Arg Tyr Thr

595 600 605

Asp Phe Ser Asn Pro Phe Ser Phe Arg Ala Asn Pro Asp Ile Ile Gly

610 615 620

Ile Ser Glu Gln Pro Leu Phe Gly Ala Gly Ser Ile Ser Ser Gly Glu

625 630 635 640

Leu Tyr Ile Asp Lys Ile Glu Ile Ile Leu Ala Asp Ala Thr Phe Glu

645 650 655

Ala Glu Ser Asp Leu Glu Arg Ala Gln Lys Ala Val Asn Glu Leu Phe

660 665 670

Thr Ser Ser Asn Gln Ile Gly Leu Lys Thr Asp Val Thr Asp Tyr His

675 680 685

Ile Asp Gln Val Ser Asn Leu Val Glu Cys Leu Ser Asp Glu Phe Cys

690 695 700

Leu Asp Glu Lys Lys Glu Leu Ser Glu Lys Val Lys His Ala Lys Arg

705 710 715 720

Leu Ser Asp Glu Arg Asn Leu Leu Gln Asp Pro Asn Phe Arg Gly Ile

725 730 735

Asn Arg Gln Leu Asp Arg Gly Trp Arg Gly Ser Thr Asp Ile Thr Ile

740 745 750

Gln Gly Gly Asp Asp Val Phe Lys Glu Asn Tyr Val Thr Leu Leu Gly

755 760 765

Thr Phe Asp Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln Lys Ile Asp Glu

770 775 780

Ser Lys Leu Lys Ala Tyr Thr Arg Tyr Gln Leu Arg Gly Tyr Ile Glu

785 790 795 800

Asp Ser Gln Asp Leu Glu Ile Tyr Leu Ile Arg Tyr Asn Ala Lys His

805 810 815

Glu Thr Val Asn Val Pro Gly Thr Gly Ser Leu Trp Pro Leu Ser Ala

820 825 830

Pro Ser Pro Ile Gly Lys Cys Ala His His Ser His His Phe Ser Leu

835 840 845

Asp Ile Asp Val Gly Cys Thr Asp Leu Asn Glu Asp Leu Gly Val Trp

850 855 860

Val Ile Phe Lys Ile Lys Thr Gln Asp Gly His Ala Arg Leu Gly Asn

865 870 875 880

Leu Glu Phe Leu Glu Glu Lys Pro Leu Val Gly Glu Ala Leu Ala Arg

885 890 895

Val Lys Arg Ala Glu Lys Lys Trp Arg Asp Lys Arg Glu Lys Leu Glu

900 905 910

Trp Glu Thr Asn Ile Val Tyr Lys Glu Ala Lys Glu Ser Val Asp Ala

915 920 925

Leu Phe Val Asn Ser Gln Tyr Asp Arg Leu Gln Ala Asp Thr Asn Ile

930 935 940

Ala Met Ile His Ala Ala Asp Lys Arg Val His Ser Ile Arg Glu Ala

945 950 955 960

Tyr Leu Pro Glu Leu Ser Val Ile Pro Gly Val Asn Ala Ala Ile Phe

965 970 975

Glu Glu Leu Glu Gly Arg Ile Phe Thr Ala Phe Ser Leu Tyr Asp Ala

980 985 990

Arg Asn Val Ile Lys Asn Gly Asp Phe Asn Asn Gly Leu Ser Cys Trp

995 1000 1005

Asn Val Lys Gly His Val Asp Val Glu Glu Gln Asn Asn His Arg

1010 1015 1020

Ser Val Leu Val Val Pro Glu Trp Glu Ala Glu Val Ser Gln Glu

1025 1030 1035

Val Arg Val Cys Pro Gly Arg Gly Tyr Ile Leu Arg Val Thr Ala

1040 1045 1050

Tyr Lys Glu Gly Tyr Gly Glu Gly Cys Val Thr Ile His Glu Ile

1055 1060 1065

Glu Asn Asn Thr Asp Glu Leu Lys Phe Ser Asn Cys Val Glu Glu

1070 1075 1080

Glu Val Tyr Pro Asn Asn Thr Val Thr Cys Asn Asp Tyr Thr Ala

1085 1090 1095

Thr Gln Glu Glu Tyr Glu Gly Thr Tyr Thr Ser Arg Asn Arg Gly

1100 1105 1110

Tyr Asp Gly Ala Tyr Glu Ser Asn Ser Ser Val Pro Ala Asp Tyr

1115 1120 1125

Ala Ser Ala Tyr Glu Glu Lys Ala Tyr Thr Asp Gly Arg Arg Asp

1130 1135 1140

Asn Pro Cys Glu Ser Asn Arg Gly Tyr Gly Asp Tyr Thr Pro Leu

1145 1150 1155

Pro Ala Gly Tyr Val Thr Lys Glu Leu Glu Tyr Phe Pro Glu Thr

1160 1165 1170

Asp Lys Val Trp Ile Glu Ile Gly Glu Thr Glu Gly Thr Phe Ile

1175 1180 1185

Val Asp Ser Val Glu Leu Leu Leu Met Glu Glu

1190 1195

<210> 48

<211> 3432

<212> DNA

<213> 人工的

<220>

<223> 用于在细菌细胞中表达的编码TIC869的重组核苷酸序列。

<400> 48

atggagataa ataatcagaa gcaatgcata ccatataatt gcttaagtaa tcctgaggaa 60

gtacttttgg atggggagag gatattacct gatatcgatc cactcgaagt ttctttgtcg 120

cttttgcaat ttcttttgaa taactttgtt ccagggggag gctttatttc aggattagtt 180

gataaaatat ggggggcttt gagaccatct gaatgggact tatttcttgc acagattgaa 240

cggttgattg atcaaagaat agaagcaaca gtaagagcaa aagcaatcac tgaattagaa 300

ggattaggga gaaattatca aatatacgct gaagcattta aagaatggga atcagatcct 360

gataacgaag cggctaaaag tagagtaatt gatcgctttc gtatacttga tggtctaatt 420

gaagcaaata tcccttcatt tcggataatt ggatttgaag tgccactttt atcggtttat 480

gttcaagcag ctaatctaca tctcgctcta ttgagagatt ctgttatttt tggagagaga 540

tggggattga cgacaaaaaa tgtcaatgat atctataata gacaaattag agaaattcat 600

gaatatagca atcattgcgt agatacgtat aacacagaac tagaacgtct agggtttaga 660

tctatagcgc agtggagaat atataatcag tttagaagag aactaacact aactgtatta 720

gatattgtcg ctcttttccc gaactatgac agtagactgt atccgatcca aactttttct 780

caattgacaa gagaaattgt tacatcccca gtaagcgaat tttattatgg tgttattaat 840

agtggtaata taattggtac tcttactgaa cagcagataa ggcgaccaca tcttatggac 900

ttctttaact ccatgatcat gtatacatca gataatagac gggaacatta ttggtcagga 960

cttgaaatga cggcttattt tacaggattt gcaggagctc aagtgtcatt ccctttagtc 1020

gggactagag gggagtcagc tccaccatta actgttagaa gtgttaatga tggaatttat 1080

agaatattat cggcaccgtt ttattcagcg ccttttctag gcaccattgt attgggaagt 1140

cgtggagaaa aatttgattt tgcgcttaat aatatttcac ctccgccatc tacaatatac 1200

agacatcctg gaacagtaga ttcactagtc agtataccgc cacaggataa tagcgtacca 1260

ccgcacaggg gatctagtca tcgattaagt catgttacaa tgcgcgcaag ttcccctata 1320

ttccattgga cgcatcgcag cgcaaccact acaaatacaa ttaatccaaa tgctattatc 1380

caaataccac tagtaaaagc atttaacctt cattcaggtg ccactgttgt tagaggacca 1440

gggtttacag gtggagatct cttacgaaga acgaatactg gtacatttgc agacataaga 1500

gtcaatgttc cttcatcact attttcccaa agatatcgcg taaggattcg ttatgcttct 1560

actaccgatt tacaattttt cacgagaatt aatggaactt ctgttaatca aggtaatttc 1620

tcaaaaacga tggatagagg ggataaactg aaatctgaaa actttagaac tgccggattt 1680

agtactcctt ttagattttc aaattttcaa agtacattca cgttgggtac tcaggctttt 1740

tcaaatcagg aagtttatat agatagaatt gaatttgtcc cggcagaagt aacattcgag 1800

gcagaatctg atttagaaag agcacaaaag gcggtgaatg agctgtttac ttcttccaat 1860

caaatcgggt taaaaacaga tgtgacggat tatcatattg atcaagtatc caatttagtt 1920

gagtgtttat ctgatgaatt ttgtctggat gaaaaaaaag aattgtccga gaaagtcaaa 1980

catgcgaagc gacttagtga tgagcggaat ttacttcaag atccaaactt tagagggatc 2040

aatagacaac tagaccgtgg ctggagagga agtacggata ttaccatcca aggaggcgat 2100

gacgtattca aagagaatta cgttacgcta ttgggtacct ttgatgagtg ctatccaacg 2160

tatttatatc aaaaaataga tgagtcgaaa ttaaaagcct atacccgtta ccaattaaga 2220

gggtatatcg aagatagtca agacttagaa atctatttaa ttcgctacaa tgccaaacac 2280

gaaacagtaa atgtgccagg tacgggttcc ttatggccgc tttcagcccc aagtccaatc 2340

ggaaaatgtg cccatcattc ccatcatttc tccttggaca ttgatgttgg atgtacagac 2400

ttaaatgagg acttaggtgt atgggtgata ttcaagatta agacgcaaga tggccatgca 2460

agactaggaa atctagaatt tctcgaagag aaaccattag taggagaagc actagctcgt 2520

gtgaaaagag cggagaaaaa atggagagac aaacgtgaaa aattggaatg ggaaacaaat 2580

attgtttata aagaggcaaa agaatctgta gatgctttat ttgtaaactc tcaatatgat 2640

agattacaag cggataccaa catcgcgatg attcatgcgg cagataaacg cgttcatagc 2700

attcgagaag cttatctgcc tgagctgtct gtgattccgg gtgtcaatgc ggctattttt 2760

gaagaattag aagggcgtat tttcactgca ttctccctat atgatgcgag aaatgtcatt 2820

aaaaatggtg attttaataa tggcttatcc tgctggaacg tgaaagggca tgtagatgta 2880

gaagaacaaa acaaccaccg ttcggtcctt gttgttccgg aatgggaagc agaagtgtca 2940

caagaagttc gtgtctgtcc gggtcgtggc tatatccttc gtgtcacagc gtacaaggag 3000

ggatatggag aaggttgcgt aaccattcat gagatcgaga acaatacaga cgaactgaag 3060

tttagcaact gtgtagaaga ggaagtatat ccaaacaaca cggtaacgtg taatgattat 3120

actgcgactc aagaagaata tgagggtacg tacacttctc gtaatcgagg atatgacgga 3180

gcctatgaaa gcaattcttc tgtaccagct gattatgcat cagcctatga agaaaaagca 3240

tatacagatg gacgaagaga caatccttgt gaatctaaca gaggatatgg ggattacaca 3300

ccactaccag ctggctatgt gacaaaagaa ttagagtact tcccagaaac cgataaggta 3360

tggattgaga tcggagaaac ggaaggaaca ttcatcgtgg acagcgtgga attacttctt 3420

atggaggaat ag 3432

<210> 49

<211> 3432

<212> DNA

<213> 人工的

<220>

<223> 设计用于在植物细胞中表达的编码TIC869的合成核苷酸序列。

<400> 49

atggagataa acaaccagaa gcagtgcatt ccgtacaact gcctcagcaa cccggaggag 60

gtgctgctgg acggcgagcg tatcctccca gacatcgacc cactggaggt cagcctgagc 120

ctcctccagt tcctcctcaa taacttcgtg ccaggcggcg gcttcatctc cggcctggtg 180

gacaagatct ggggcgcact ccggccaagt gagtgggatc tgttcctggc ccaaatcgag 240

cgcctgatcg accagaggat cgaggcgacg gtccgcgcca aggcgataac cgagctggag 300

ggcctcggtc gcaactacca gatctacgca gaggcgttca aggagtggga gagcgacccg 360

gacaacgagg cggccaagtc tcgggtgatt gaccgcttcc gcatcctcga cggcctcatc 420

gaagccaaca tcccttcctt ccggatcata ggcttcgaag tcccgctcct cagcgtgtac 480

gtgcaagcgg ccaatctcca cctcgcgttg ctccgtgaca gcgtcatctt tggcgagaga 540

tggggcctga cgacgaagaa cgtgaacgac atctacaaca ggcagatccg agagattcac 600

gagtacagca accactgcgt ggacacatac aacacggagc tggagcggct cggcttccgc 660

tcaatcgctc agtggcggat ctacaaccag ttccgccgcg agctgaccct caccgtgctc 720

gacatcgtcg cattgtttcc caattacgac tcacgcctct acccaatcca gactttcagc 780

cagctcacac gcgagattgt gaccagcccg gtgtcagagt tctactacgg cgtcatcaac 840

tcaggcaaca tcatcgggac actgactgaa cagcagatca gacgtccgca cttgatggac 900

ttcttcaact ccatgattat gtacacatca gacaacagga gagagcacta ctggtccggg 960

ttggagatga ctgcttactt caccggcttc gccggtgccc aagtgagctt cccactggtc 1020

ggaactcgtg gcgagtcagc tcctccgcta actgtgcgat ctgtcaacga cgggatctac 1080

agaatactgt cggctccctt ctacagtgcg ccgttcctcg gcaccatcgt cctcggctca 1140

cgtggtgaga agttcgactt cgcactgaac aacattagcc cgccgcctag tacaatctac 1200

aggcaccctg gcaccgtgga ctcactggtt tcgatcccgc cacaagacaa cagtgtgccg 1260

ccacatcgtg gttctagcca caggctctcc catgtgacca tgcgcgcctc ttcaccgatc 1320

tttcactgga cccatcggtc cgctacaacc acaaacacca tcaaccctaa cgccatcatc 1380

caaatcccgc tggtgaaggc gtttaacctc cacagcggcg caactgtcgt gcgcggccct 1440

ggattcaccg gtggtgacct gctccgtcgg accaatactg gcacgttcgc agacatccga 1500

gtgaacgtcc cgtcctcgct gttcagtcag cgctaccgtg tccgcattcg gtacgcttcc 1560

accacggatc tccagttctt tactcgcatc aatgggacga gcgtcaacca gggcaacttc 1620

agcaagacga tggaccgtgg agataagctc aagtccgaga acttccgcac ggctggcttc 1680

tcgacaccgt tcagattcag caacttccag agcactttca cgctgggcac acaggcgttc 1740

tccaaccagg aggtgtacat cgaccgcatc gagttcgtgc ctgctgaggt taccttcgag 1800

gcggaaagcg acctcgaaag ggcccagaag gccgtcaacg agctgttcac ctccagcaac 1860

cagatcggtc tcaagaccga cgtcactgac tatcacattg accaagtcag caacctggtg 1920

gagtgcctca gtgatgagtt ctgcctggat gagaagaagg agcttagcga gaaggtcaag 1980

cacgcaaagc gcttgagcga cgagcgcaac cttctccagg acccgaattt ccgtggtatc 2040

aatagacagc ttgaccgtgg gtggcgcggt agtaccgaca taaccatcca gggtggcgac 2100

gatgtgttca aggagaatta tgttacgctg ctcggtacgt tcgacgagtg ctatcccacg 2160

tacttgtacc agaagattga cgagagcaag ctcaaggcgt acacccgtta ccagctccgt 2220

ggctacatcg aggacagcca ggatctggaa atctacctta tccgatacaa tgctaagcac 2280

gagacagtca acgtgcccgg aacagggtcg ctctggccgc tcagtgctcc gtcgcccatt 2340

ggcaagtgcg cgcaccattc gcatcacttc tcacttgaca ttgacgtggg ctgcaccgac 2400

ctgaacgagg atctgggtgt ctgggtcatc ttcaagatca agacccaaga cggccacgcg 2460

cgcctcggga acctggagtt cctggaggag aagcctttgg taggtgaagc cctggcccgc 2520

gtcaagcgcg cggagaagaa gtggcgcgac aagagggaga agctggaatg ggagaccaac 2580

atcgtgtaca aggaggcgaa ggagtcggtg gacgcactat tcgtgaactc ccagtacgac 2640

cgtctccagg ccgacaccaa catcgccatg atccacgccg ctgacaaacg agttcattcc 2700

attcgtgaag cctatcttcc cgagctgtct gtcataccgg gcgtcaacgc ggccatcttc 2760

gaggagttag agggtcggat ctttacagct ttctcactgt acgatgcccg caacgtcatc 2820

aagaacggcg acttcaacaa cggtctctcc tgttggaacg tgaagggcca cgtggatgtc 2880

gaggagcaga acaaccaccg ctctgtgctt gtggtgcccg agtgggaggc cgaggtgagc 2940

caggaggtcc gcgtctgtcc gggtcgcggc tacatcctgc gggtcaccgc ctacaaggag 3000

ggctacggcg aaggctgcgt tactattcac gagattgaga acaataccga cgaactcaag 3060

ttctccaact gtgtcgagga ggaggtgtac ccgaacaaca ccgtgacgtg caacgactac 3120

accgcgacac aggaggaata cgagggcacc tacaccagcc gcaaccgagg ctacgacgga 3180

gcgtacgaga gcaactcgtc cgtgcccgct gattacgcga gtgcgtacga ggagaaggct 3240

tacaccgacg gacggcgcga caatccctgc gagagtaacc gtggatacgg agattacacg 3300

ccgctacccg ctggctacgt cactaaggaa ctggagtact tcccagagac ggacaaggtg 3360

tggatcgaaa tcggcgagac agagggcacg ttcatcgtgg actccgtgga gctgctgctg 3420

atggaggagt ga 3432

<210> 50

<211> 1143

<212> PRT

<213> 人工的

<220>

<223> 嵌合蛋白质TIC869的氨基酸序列。

<400> 50

Met Glu Ile Asn Asn Gln Lys Gln Cys Ile Pro Tyr Asn Cys Leu Ser

1 5 10 15

Asn Pro Glu Glu Val Leu Leu Asp Gly Glu Arg Ile Leu Pro Asp Ile

20 25 30

Asp Pro Leu Glu Val Ser Leu Ser Leu Leu Gln Phe Leu Leu Asn Asn

35 40 45

Phe Val Pro Gly Gly Gly Phe Ile Ser Gly Leu Val Asp Lys Ile Trp

50 55 60

Gly Ala Leu Arg Pro Ser Glu Trp Asp Leu Phe Leu Ala Gln Ile Glu

65 70 75 80

Arg Leu Ile Asp Gln Arg Ile Glu Ala Thr Val Arg Ala Lys Ala Ile

85 90 95

Thr Glu Leu Glu Gly Leu Gly Arg Asn Tyr Gln Ile Tyr Ala Glu Ala

100 105 110

Phe Lys Glu Trp Glu Ser Asp Pro Asp Asn Glu Ala Ala Lys Ser Arg

115 120 125

Val Ile Asp Arg Phe Arg Ile Leu Asp Gly Leu Ile Glu Ala Asn Ile

130 135 140

Pro Ser Phe Arg Ile Ile Gly Phe Glu Val Pro Leu Leu Ser Val Tyr

145 150 155 160

Val Gln Ala Ala Asn Leu His Leu Ala Leu Leu Arg Asp Ser Val Ile

165 170 175

Phe Gly Glu Arg Trp Gly Leu Thr Thr Lys Asn Val Asn Asp Ile Tyr

180 185 190

Asn Arg Gln Ile Arg Glu Ile His Glu Tyr Ser Asn His Cys Val Asp

195 200 205

Thr Tyr Asn Thr Glu Leu Glu Arg Leu Gly Phe Arg Ser Ile Ala Gln

210 215 220

Trp Arg Ile Tyr Asn Gln Phe Arg Arg Glu Leu Thr Leu Thr Val Leu

225 230 235 240

Asp Ile Val Ala Leu Phe Pro Asn Tyr Asp Ser Arg Leu Tyr Pro Ile

245 250 255

Gln Thr Phe Ser Gln Leu Thr Arg Glu Ile Val Thr Ser Pro Val Ser

260 265 270

Glu Phe Tyr Tyr Gly Val Ile Asn Ser Gly Asn Ile Ile Gly Thr Leu

275 280 285

Thr Glu Gln Gln Ile Arg Arg Pro His Leu Met Asp Phe Phe Asn Ser

290 295 300

Met Ile Met Tyr Thr Ser Asp Asn Arg Arg Glu His Tyr Trp Ser Gly

305 310 315 320

Leu Glu Met Thr Ala Tyr Phe Thr Gly Phe Ala Gly Ala Gln Val Ser

325 330 335

Phe Pro Leu Val Gly Thr Arg Gly Glu Ser Ala Pro Pro Leu Thr Val

340 345 350

Arg Ser Val Asn Asp Gly Ile Tyr Arg Ile Leu Ser Ala Pro Phe Tyr

355 360 365

Ser Ala Pro Phe Leu Gly Thr Ile Val Leu Gly Ser Arg Gly Glu Lys

370 375 380

Phe Asp Phe Ala Leu Asn Asn Ile Ser Pro Pro Pro Ser Thr Ile Tyr

385 390 395 400

Arg His Pro Gly Thr Val Asp Ser Leu Val Ser Ile Pro Pro Gln Asp

405 410 415

Asn Ser Val Pro Pro His Arg Gly Ser Ser His Arg Leu Ser His Val

420 425 430

Thr Met Arg Ala Ser Ser Pro Ile Phe His Trp Thr His Arg Ser Ala

435 440 445

Thr Thr Thr Asn Thr Ile Asn Pro Asn Ala Ile Ile Gln Ile Pro Leu

450 455 460

Val Lys Ala Phe Asn Leu His Ser Gly Ala Thr Val Val Arg Gly Pro

465 470 475 480

Gly Phe Thr Gly Gly Asp Leu Leu Arg Arg Thr Asn Thr Gly Thr Phe

485 490 495

Ala Asp Ile Arg Val Asn Val Pro Ser Ser Leu Phe Ser Gln Arg Tyr

500 505 510

Arg Val Arg Ile Arg Tyr Ala Ser Thr Thr Asp Leu Gln Phe Phe Thr

515 520 525

Arg Ile Asn Gly Thr Ser Val Asn Gln Gly Asn Phe Ser Lys Thr Met

530 535 540

Asp Arg Gly Asp Lys Leu Lys Ser Glu Asn Phe Arg Thr Ala Gly Phe

545 550 555 560

Ser Thr Pro Phe Arg Phe Ser Asn Phe Gln Ser Thr Phe Thr Leu Gly

565 570 575

Thr Gln Ala Phe Ser Asn Gln Glu Val Tyr Ile Asp Arg Ile Glu Phe

580 585 590

Val Pro Ala Glu Val Thr Phe Glu Ala Glu Ser Asp Leu Glu Arg Ala

595 600 605

Gln Lys Ala Val Asn Glu Leu Phe Thr Ser Ser Asn Gln Ile Gly Leu

610 615 620

Lys Thr Asp Val Thr Asp Tyr His Ile Asp Gln Val Ser Asn Leu Val

625 630 635 640

Glu Cys Leu Ser Asp Glu Phe Cys Leu Asp Glu Lys Lys Glu Leu Ser

645 650 655

Glu Lys Val Lys His Ala Lys Arg Leu Ser Asp Glu Arg Asn Leu Leu

660 665 670

Gln Asp Pro Asn Phe Arg Gly Ile Asn Arg Gln Leu Asp Arg Gly Trp

675 680 685

Arg Gly Ser Thr Asp Ile Thr Ile Gln Gly Gly Asp Asp Val Phe Lys

690 695 700

Glu Asn Tyr Val Thr Leu Leu Gly Thr Phe Asp Glu Cys Tyr Pro Thr

705 710 715 720

Tyr Leu Tyr Gln Lys Ile Asp Glu Ser Lys Leu Lys Ala Tyr Thr Arg

725 730 735

Tyr Gln Leu Arg Gly Tyr Ile Glu Asp Ser Gln Asp Leu Glu Ile Tyr

740 745 750

Leu Ile Arg Tyr Asn Ala Lys His Glu Thr Val Asn Val Pro Gly Thr

755 760 765

Gly Ser Leu Trp Pro Leu Ser Ala Pro Ser Pro Ile Gly Lys Cys Ala

770 775 780

His His Ser His His Phe Ser Leu Asp Ile Asp Val Gly Cys Thr Asp

785 790 795 800

Leu Asn Glu Asp Leu Gly Val Trp Val Ile Phe Lys Ile Lys Thr Gln

805 810 815

Asp Gly His Ala Arg Leu Gly Asn Leu Glu Phe Leu Glu Glu Lys Pro

820 825 830

Leu Val Gly Glu Ala Leu Ala Arg Val Lys Arg Ala Glu Lys Lys Trp

835 840 845

Arg Asp Lys Arg Glu Lys Leu Glu Trp Glu Thr Asn Ile Val Tyr Lys

850 855 860

Glu Ala Lys Glu Ser Val Asp Ala Leu Phe Val Asn Ser Gln Tyr Asp

865 870 875 880

Arg Leu Gln Ala Asp Thr Asn Ile Ala Met Ile His Ala Ala Asp Lys

885 890 895

Arg Val His Ser Ile Arg Glu Ala Tyr Leu Pro Glu Leu Ser Val Ile

900 905 910

Pro Gly Val Asn Ala Ala Ile Phe Glu Glu Leu Glu Gly Arg Ile Phe

915 920 925

Thr Ala Phe Ser Leu Tyr Asp Ala Arg Asn Val Ile Lys Asn Gly Asp

930 935 940

Phe Asn Asn Gly Leu Ser Cys Trp Asn Val Lys Gly His Val Asp Val

945 950 955 960

Glu Glu Gln Asn Asn His Arg Ser Val Leu Val Val Pro Glu Trp Glu

965 970 975

Ala Glu Val Ser Gln Glu Val Arg Val Cys Pro Gly Arg Gly Tyr Ile

980 985 990

Leu Arg Val Thr Ala Tyr Lys Glu Gly Tyr Gly Glu Gly Cys Val Thr

995 1000 1005

Ile His Glu Ile Glu Asn Asn Thr Asp Glu Leu Lys Phe Ser Asn

1010 1015 1020

Cys Val Glu Glu Glu Val Tyr Pro Asn Asn Thr Val Thr Cys Asn

1025 1030 1035

Asp Tyr Thr Ala Thr Gln Glu Glu Tyr Glu Gly Thr Tyr Thr Ser

1040 1045 1050

Arg Asn Arg Gly Tyr Asp Gly Ala Tyr Glu Ser Asn Ser Ser Val

1055 1060 1065

Pro Ala Asp Tyr Ala Ser Ala Tyr Glu Glu Lys Ala Tyr Thr Asp

1070 1075 1080

Gly Arg Arg Asp Asn Pro Cys Glu Ser Asn Arg Gly Tyr Gly Asp

1085 1090 1095

Tyr Thr Pro Leu Pro Ala Gly Tyr Val Thr Lys Glu Leu Glu Tyr

1100 1105 1110

Phe Pro Glu Thr Asp Lys Val Trp Ile Glu Ile Gly Glu Thr Glu

1115 1120 1125

Gly Thr Phe Ile Val Asp Ser Val Glu Leu Leu Leu Met Glu Glu

1130 1135 1140

<210> 51

<211> 3513

<212> DNA

<213> 人工的

<220>

<223> 用于在细菌细胞中表达的编码TIC836的重组核苷酸序列。

<400> 51

atggagaata atattcaaaa tcaatgcgta ccttacaatt gtttaaataa tcctgaagta 60

gaaatattaa atgaagaaag aagtactggc agattaccgt tagatatatc cttatcgctt 120

acacgtttcc ttttgagtga atttgttcca ggtgtgggag ttgcgtttgg attatttgat 180

ttaatatggg gttttataac tccttctgat tggagcttat ttcttttaca gattgaacaa 240

ttgattgagc aaagaataga aacattggaa aggaaccggg caattactac attacgaggg 300

ttagcagata gctatgaaat ttatattgaa gcactaagag agtgggaagc aaatcctaat 360

aatgcacaat taagggaaga tgtgcgtatt cgatttgcta atacagacga cgctttaata 420

acagcaataa ataattttac acttacaagt tttgaaatcc ctcttttatc ggtctatgtt 480

caagcggcga atttacattt atcactatta agagacgctg tatcgtttgg gcagggttgg 540

ggactggata tagctactgt taataatcat tataatagat taataaatct tattcataga 600

tatacgaaac attgtttgga cacatacaat caaggattag aaaacttaag aggtactaat 660

actcgacaat gggcaagatt caatcagttt aggagagatt taacacttac tgtattagat 720

atcgttgctc tttttccgaa ctacgatgtt agaacatatc caattcaaac gtcatcccaa 780

ttaacaaggg aaatttatac aagttcagta attgaggatt ctccagtttc tgctaatata 840

cctaatggtt ttaatagggc ggaatttgga gttagaccgc cccatcttat ggactttatg 900

aattctttgt ttgtaactgc agagactgtt agaagtcaaa ctgtgtgggg aggacactta 960

gttagttcac gaaatacggc tggtaaccgt ataaatttcc ctagttacgg ggtcttcaat 1020

cctggtggcg ccatttggat tgcagatgag gatccacgtc ctttttatcg gacattatca 1080

gatcctgttt ttgtccgagg aggatttggg aatcctcatt atgtactggg gcttagggga 1140

gtagcatttc aacaaactgg tacgaaccac acccgaacat ttagaaatag tgggaccata 1200

gattctctag atgaaatccc acctcaggat aatagtgggg caccttggaa tgattatagt 1260

catgtattaa atcatgttac atttgtacga tggccaggtg agatttcagg aagtgattca 1320

tggagagctc caatgttttc ttggacgcac cgtagtgcaa cccctacaaa tacaattgat 1380

ccggagagga ttacacaaat acctttaaca aaatctacta atcttggctc tggaacttct 1440

gtcgttaaag gaccaggatt tacaggagga gatattcttc gaagaacttc acctggccag 1500

atttcaacct taagagtaaa tattactgca ccattatcac aaagatatcg ggtaagaatt 1560

cgctacgctt ctaccacaaa tttacaattc catacatcaa ttgacggaag acctattaat 1620

caggggaatt tttcagcaac tatgagtagt gggagtaatt tacagtccgg aagctttagg 1680

actgtaggtt ttactactcc gtttaacttt tcaaatggat caagtgtatt tacgttaagt 1740

gctcatgtct tcaattcagg caatgaagtt tatatagatc gaattgaatt tgttccggca 1800

gaagtaacct ttgaggcaga atatgattta gaaagagcgc agaaggcggt gaatgcgctg 1860

tttacgtcta caaaccaact agggctaaaa acaaatgtaa cggattatca tattgatcaa 1920

gtgtccaatt tagttacgta tttatcggat gaattttgtc tggatgaaaa gcgagaattg 1980

tccgagaaag tcaaacatgc gaagcgactc agtgatgaac gcaatttact ccaagattca 2040

aatttcaaag acattaatag gcaaccagaa cgtgggtggg gcggaagtac agggattacc 2100

atccaaggag gggatgacgt atttaaagaa aattacgtca cactatcagg tacctttgat 2160

gagtgctatc caacatattt gtatcaaaaa atcgatgaat caaaattaaa agcctttacc 2220

cgttatcaat taagagggta tatcgaagat agtcaagact tagaaatcta tttaattcgc 2280

tacaatgcaa aacatgaaac agtaaatgtg ccaggtacgg gttccttatg gccgctttca 2340

gcccaaagtc caatcggaaa gtgtggagag ccgaatcgat gcgcgccaca ccttgaatgg 2400

aatcctgact tagattgttc gtgtagggat ggagaaaagt gtgcccatca ttcgcatcat 2460

ttctccttag acattgatgt aggatgtaca gacttaaatg aggacctagg tgtatgggtg 2520

atctttaaga ttaagacgca agatgggcac gcaagactag ggaatctaga gtttctcgaa 2580

gaaaaaccat tagtaggaga agcgctagct cgtgtgaaaa gagcggagaa aaaatggaga 2640

gacaaacgtg aaaaattgga atgggaaaca aatatcgttt ataaagaggc aaaagaatct 2700

gtagatgctt tatttgtaaa ctctcaatat gatcaattac aagcggatac gaatattgcc 2760

atgattcatg cggcagataa acgtgttcat agcattcgag aagcttatct gcctgagctg 2820

tctgtgattc cgggtgtcaa tgcggctatt tttgaagaat tagaagggcg tattttcact 2880

gcattctccc tatatgatgc gagaaatgtc attaaaaatg gtgattttaa taatggctta 2940

tcctgctgga acgtgaaagg gcatgtagat gtagaagaac aaaacaacca acgttcggtc 3000

cttgttgttc cggaatggga agcagaagtg tcacaagaag ttcgtgtctg tccgggtcgt 3060

ggctatatcc ttcgtgtcac agcgtacaag gagggatatg gagaaggttg cgtaaccatt 3120

catgagatcg agaacaatac agacgaactg aagtttagca actgcgtaga ggaggaaatc 3180

tatccaaata acacggtaac gtgtaatgat tatactgtaa atcaagaaga atacggaggt 3240

gcgtacactt ctcgtaatcg aggatataac gaagctcctt ccgtaccagc tgattatgcg 3300

tcagtctatg aagaaaaatc gtatacagat ggacgtagag agaatccttg tgaatttaac 3360

agagggtata gggattacac gccactacca gttggttatg tgacaaaaga attagaatac 3420

ttcccagaaa ccgataaggt atggattgag attggagaaa cggaaggaac atttatcgtg 3480

gacagcgtgg aattactcct tatggaggaa taa 3513

<210> 52

<211> 3513

<212> DNA

<213> 人工的

<220>

<223> 设计用于在植物细胞中表达的编码TIC836的合成核苷酸序列。

<400> 52

atggagaaca acatccagaa ccagtgcgtg ccctacaact gcctgaacaa ccctgaggtt 60

gagatcctga acgaggagcg tagcaccggt aggctcccgc tagacatctc cctgagcctg 120

acccgcttcc tccttagtga gttcgtgccc ggcgtgggcg tggccttcgg cctcttcgac 180

ctcatctggg gcttcatcac tccttccgac tggtccctct tcctccttca gattgagcaa 240

ctgatcgagc agcgcatcga gacccttgag cgcaaccgcg ccatcaccac tctcagaggt 300

ctcgccgact cctacgaaat ctacatcgag gcactccgtg agtgggaggc caacccgaac 360

aatgcccagc tccgcgagga cgtgaggatc agattcgcca acaccgacga tgccctcatc 420

accgccatca acaatttcac cctcacctcc ttcgagatcc ctcttctgtc tgtgtacgtt 480

caagctgcta accttcacct ttccctcctg cgcgacgccg tgagcttcgg ccagggctgg 540

ggcctcgaca tcgccaccgt gaacaatcac tacaaccgcc tcatcaacct catccaccgc 600

tacaccaagc actgccttga cacctacaac cagggccttg agaacctccg tggcaccaac 660

acccgccagt gggcccgctt caaccagttc cgcagagacc tcaccctcac cgtgctcgac 720

atcgtggcac tcttcccaaa ctacgacgtg cgtacctacc ctatccagac ctccagccag 780

ctcaccaggg aaatctacac ctccagcgtg atcgaggact ctcctgtgtc cgccaacatc 840

cctaacggct tcaaccgcgc cgagttcggc gtgcgccctc ctcacctcat ggacttcatg 900

aactccctct tcgtcactgc cgagaccgtg cgctcccaga ccgtgtgggg cggtcacctc 960

gtgtccagcc gtaacaccgc tggcaacagg atcaacttcc cgtcctacgg cgtgttcaac 1020

ccaggcggtg ccatctggat cgccgatgaa gaccctcgtc ctttctaccg taccctgtcc 1080

gaccctgtgt tcgtgcgtgg cggtttcggc aaccctcact acgtgctggg cctgcgtggc 1140

gtggccttcc agcaaaccgg caccaaccac accaggacgt tccgtaactc cggcaccatc 1200

gacagtcttg acgagatccc tccgcaagac aactccggtg caccttggaa cgactactcc 1260

cacgtgctga accacgtgac cttcgtgagg tggcctggcg aaatctccgg ctccgactcc 1320

tggagggctc ctatgttcag ttggacccac aggagcgcta cgcctaccaa caccatcgac 1380

cctgagcgta tcactcagat ccctctgact aagagcacta acctgggcag cggcactagc 1440

gtggtcaagg gccctggctt cactggcggt gacatcctga ggcggactag ccctggccag 1500

atcagcactc tgagggtgaa catcactgct ccgctgagcc agcgttacag ggtcagaatc 1560

cgttacgctt ctactactaa ccttcagttc cacactagca tcgacggccg tccgatcaac 1620

cagggcaact tctctgctac tatgagttct ggcagtaacc tccagtctgg tagtttccgg 1680

actgtcggtt tcactacgcc gttcaacttc tccaacggta gttctgtctt cactctgtct 1740

gctcacgtgt tcaactctgg caacgaggtg tacatcgacc ggatcgagtt cgtccctgct 1800

gaggtgacgt tcgaggccga gtacgacctg gagcgggctc agaaggctgt caacgctctg 1860

ttcacttcta ctaaccagct tggtttgaag actaacgtga ccgactacca cattgatcaa 1920

gtcagtaacc tggtcacgta cctgtctgac gagttctgtc ttgacgagaa gcgggagctg 1980

tctgagaagg tcaagcacgc taagcggctg tctgacgagc ggaacctgct tcaagacagt 2040

aacttcaagg acattaaccg ccagcctgag cgtggttggg gagggtccac gggtattacg 2100

attcaaggag gtgacgatgt ctttaaggag aactatgtga cgctttcggg tacgtttgat 2160

gagtgctatc caacgtacct ttaccagaag attgacgagt cgaagctgaa ggctttcact 2220

cgttaccagc ttcgtggtta cattgaggac tcgcaagacc tcgaaatcta cctcattcgt 2280

tacaacgcta agcacgagac tgtcaacgtc cctggtacgg gtagtctttg gccgctttct 2340

gctcagtcgc cgattggcaa gtgtggcgag ccgaaccgtt gcgctcctca cttggagtgg 2400

aacccggatc tcgattgctc gtgccgtgac ggtgagaagt gcgcgcacca tagtcatcac 2460

tttagccttg acattgatgt cggttgcacg gatcttaacg aggatcttgg agtctgggtg 2520

attttcaaga tcaaaactca ggatgggcac gcgcgtcttg ggaatcttga gttcctggag 2580

gagaagccac ttgtcggtga ggcgcttgcg cgtgtcaagc gtgcggagaa gaaatggcgt 2640

gataagcgtg agaagttgga gtgggagacg aacatcgtgt acaaggaggc gaaggagtcg 2700

gtcgatgcgt tgtttgtcaa tagtcaatac gatcaattgc aagcggatac gaacatcgca 2760

atgattcatg cggcagataa gcgtgtccat tcgattcgtg aggcgtactt gccagagttg 2820

tcggtcatcc caggagttaa tgcggcaatc tttgaggaat tggagggcag aatcttcacg 2880

gcgttctcgt tgtacgatgc aagaaatgtt attaagaatg gagatttcaa caatgggttg 2940

tcatgctgga atgttaaggg tcacgttgat gttgaagaac agaacaacca gagatcagtg 3000

ttggttgtac cagagtggga ggcagaggtt tcacaagagg tgagagtttg cccaggcaga 3060

ggctacatct tgagagttac agcatacaaa gagggatacg gcgagggatg tgttacaatc 3120

cacgaaatcg agaacaatac cgatgagcta aagttctcaa attgtgttga ggaggagatc 3180

tacccgaaca acacggttac ttgtaatgat tacacagtga accaggagga gtatggtggt 3240

gcatacacat caagaaatag aggctacaat gaagcaccat cagttccagc agattatgcc 3300

tcagtttatg aggagaagtc atacacagat ggacgacgtg agaatccatg tgagttcaat 3360

cgaggatacc gagattacac accactacca gttggatacg ttacaaagga actagaatac 3420

ttcccagaaa cagataaagt atggatagag atcggagaaa cagaaggaac attcatcgtt 3480

gattcagtag aactactact tatggaagaa tga 3513

<210> 53

<211> 1170

<212> PRT

<213> 人工的

<220>

<223> 嵌合蛋白质TIC836的氨基酸序列。

<400> 53

Met Glu Asn Asn Ile Gln Asn Gln Cys Val Pro Tyr Asn Cys Leu Asn

1 5 10 15

Asn Pro Glu Val Glu Ile Leu Asn Glu Glu Arg Ser Thr Gly Arg Leu

20 25 30

Pro Leu Asp Ile Ser Leu Ser Leu Thr Arg Phe Leu Leu Ser Glu Phe

35 40 45

Val Pro Gly Val Gly Val Ala Phe Gly Leu Phe Asp Leu Ile Trp Gly

50 55 60

Phe Ile Thr Pro Ser Asp Trp Ser Leu Phe Leu Leu Gln Ile Glu Gln

65 70 75 80

Leu Ile Glu Gln Arg Ile Glu Thr Leu Glu Arg Asn Arg Ala Ile Thr

85 90 95

Thr Leu Arg Gly Leu Ala Asp Ser Tyr Glu Ile Tyr Ile Glu Ala Leu

100 105 110

Arg Glu Trp Glu Ala Asn Pro Asn Asn Ala Gln Leu Arg Glu Asp Val

115 120 125

Arg Ile Arg Phe Ala Asn Thr Asp Asp Ala Leu Ile Thr Ala Ile Asn

130 135 140

Asn Phe Thr Leu Thr Ser Phe Glu Ile Pro Leu Leu Ser Val Tyr Val

145 150 155 160

Gln Ala Ala Asn Leu His Leu Ser Leu Leu Arg Asp Ala Val Ser Phe

165 170 175

Gly Gln Gly Trp Gly Leu Asp Ile Ala Thr Val Asn Asn His Tyr Asn

180 185 190

Arg Leu Ile Asn Leu Ile His Arg Tyr Thr Lys His Cys Leu Asp Thr

195 200 205

Tyr Asn Gln Gly Leu Glu Asn Leu Arg Gly Thr Asn Thr Arg Gln Trp

210 215 220

Ala Arg Phe Asn Gln Phe Arg Arg Asp Leu Thr Leu Thr Val Leu Asp

225 230 235 240

Ile Val Ala Leu Phe Pro Asn Tyr Asp Val Arg Thr Tyr Pro Ile Gln

245 250 255

Thr Ser Ser Gln Leu Thr Arg Glu Ile Tyr Thr Ser Ser Val Ile Glu

260 265 270

Asp Ser Pro Val Ser Ala Asn Ile Pro Asn Gly Phe Asn Arg Ala Glu

275 280 285

Phe Gly Val Arg Pro Pro His Leu Met Asp Phe Met Asn Ser Leu Phe

290 295 300

Val Thr Ala Glu Thr Val Arg Ser Gln Thr Val Trp Gly Gly His Leu

305 310 315 320

Val Ser Ser Arg Asn Thr Ala Gly Asn Arg Ile Asn Phe Pro Ser Tyr

325 330 335

Gly Val Phe Asn Pro Gly Gly Ala Ile Trp Ile Ala Asp Glu Asp Pro

340 345 350

Arg Pro Phe Tyr Arg Thr Leu Ser Asp Pro Val Phe Val Arg Gly Gly

355 360 365

Phe Gly Asn Pro His Tyr Val Leu Gly Leu Arg Gly Val Ala Phe Gln

370 375 380

Gln Thr Gly Thr Asn His Thr Arg Thr Phe Arg Asn Ser Gly Thr Ile

385 390 395 400

Asp Ser Leu Asp Glu Ile Pro Pro Gln Asp Asn Ser Gly Ala Pro Trp

405 410 415

Asn Asp Tyr Ser His Val Leu Asn His Val Thr Phe Val Arg Trp Pro

420 425 430

Gly Glu Ile Ser Gly Ser Asp Ser Trp Arg Ala Pro Met Phe Ser Trp

435 440 445

Thr His Arg Ser Ala Thr Pro Thr Asn Thr Ile Asp Pro Glu Arg Ile

450 455 460

Thr Gln Ile Pro Leu Thr Lys Ser Thr Asn Leu Gly Ser Gly Thr Ser

465 470 475 480

Val Val Lys Gly Pro Gly Phe Thr Gly Gly Asp Ile Leu Arg Arg Thr

485 490 495

Ser Pro Gly Gln Ile Ser Thr Leu Arg Val Asn Ile Thr Ala Pro Leu

500 505 510

Ser Gln Arg Tyr Arg Val Arg Ile Arg Tyr Ala Ser Thr Thr Asn Leu

515 520 525

Gln Phe His Thr Ser Ile Asp Gly Arg Pro Ile Asn Gln Gly Asn Phe

530 535 540

Ser Ala Thr Met Ser Ser Gly Ser Asn Leu Gln Ser Gly Ser Phe Arg

545 550 555 560

Thr Val Gly Phe Thr Thr Pro Phe Asn Phe Ser Asn Gly Ser Ser Val

565 570 575

Phe Thr Leu Ser Ala His Val Phe Asn Ser Gly Asn Glu Val Tyr Ile

580 585 590

Asp Arg Ile Glu Phe Val Pro Ala Glu Val Thr Phe Glu Ala Glu Tyr

595 600 605

Asp Leu Glu Arg Ala Gln Lys Ala Val Asn Ala Leu Phe Thr Ser Thr

610 615 620

Asn Gln Leu Gly Leu Lys Thr Asn Val Thr Asp Tyr His Ile Asp Gln

625 630 635 640

Val Ser Asn Leu Val Thr Tyr Leu Ser Asp Glu Phe Cys Leu Asp Glu

645 650 655

Lys Arg Glu Leu Ser Glu Lys Val Lys His Ala Lys Arg Leu Ser Asp

660 665 670

Glu Arg Asn Leu Leu Gln Asp Ser Asn Phe Lys Asp Ile Asn Arg Gln

675 680 685

Pro Glu Arg Gly Trp Gly Gly Ser Thr Gly Ile Thr Ile Gln Gly Gly

690 695 700

Asp Asp Val Phe Lys Glu Asn Tyr Val Thr Leu Ser Gly Thr Phe Asp

705 710 715 720

Glu Cys Tyr Pro Thr Tyr Leu Tyr Gln Lys Ile Asp Glu Ser Lys Leu

725 730 735

Lys Ala Phe Thr Arg Tyr Gln Leu Arg Gly Tyr Ile Glu Asp Ser Gln

740 745 750

Asp Leu Glu Ile Tyr Leu Ile Arg Tyr Asn Ala Lys His Glu Thr Val

755 760 765

Asn Val Pro Gly Thr Gly Ser Leu Trp Pro Leu Ser Ala Gln Ser Pro

770 775 780

Ile Gly Lys Cys Gly Glu Pro Asn Arg Cys Ala Pro His Leu Glu Trp

785 790 795 800

Asn Pro Asp Leu Asp Cys Ser Cys Arg Asp Gly Glu Lys Cys Ala His

805 810 815

His Ser His His Phe Ser Leu Asp Ile Asp Val Gly Cys Thr Asp Leu

820 825 830

Asn Glu Asp Leu Gly Val Trp Val Ile Phe Lys Ile Lys Thr Gln Asp

835 840 845

Gly His Ala Arg Leu Gly Asn Leu Glu Phe Leu Glu Glu Lys Pro Leu

850 855 860

Val Gly Glu Ala Leu Ala Arg Val Lys Arg Ala Glu Lys Lys Trp Arg

865 870 875 880

Asp Lys Arg Glu Lys Leu Glu Trp Glu Thr Asn Ile Val Tyr Lys Glu

885 890 895

Ala Lys Glu Ser Val Asp Ala Leu Phe Val Asn Ser Gln Tyr Asp Gln

900 905 910

Leu Gln Ala Asp Thr Asn Ile Ala Met Ile His Ala Ala Asp Lys Arg

915 920 925

Val His Ser Ile Arg Glu Ala Tyr Leu Pro Glu Leu Ser Val Ile Pro

930 935 940

Gly Val Asn Ala Ala Ile Phe Glu Glu Leu Glu Gly Arg Ile Phe Thr

945 950 955 960

Ala Phe Ser Leu Tyr Asp Ala Arg Asn Val Ile Lys Asn Gly Asp Phe

965 970 975

Asn Asn Gly Leu Ser Cys Trp Asn Val Lys Gly His Val Asp Val Glu

980 985 990

Glu Gln Asn Asn Gln Arg Ser Val Leu Val Val Pro Glu Trp Glu Ala

995 1000 1005

Glu Val Ser Gln Glu Val Arg Val Cys Pro Gly Arg Gly Tyr Ile

1010 1015 1020

Leu Arg Val Thr Ala Tyr Lys Glu Gly Tyr Gly Glu Gly Cys Val

1025 1030 1035

Thr Ile His Glu Ile Glu Asn Asn Thr Asp Glu Leu Lys Phe Ser

1040 1045 1050

Asn Cys Val Glu Glu Glu Ile Tyr Pro Asn Asn Thr Val Thr Cys

1055 1060 1065

Asn Asp Tyr Thr Val Asn Gln Glu Glu Tyr Gly Gly Ala Tyr Thr

1070 1075 1080

Ser Arg Asn Arg Gly Tyr Asn Glu Ala Pro Ser Val Pro Ala Asp

1085 1090 1095

Tyr Ala Ser Val Tyr Glu Glu Lys Ser Tyr Thr Asp Gly Arg Arg

1100 1105 1110

Glu Asn Pro Cys Glu Phe Asn Arg Gly Tyr Arg Asp Tyr Thr Pro

1115 1120 1125

Leu Pro Val Gly Tyr Val Thr Lys Glu Leu Glu Tyr Phe Pro Glu

1130 1135 1140

Thr Asp Lys Val Trp Ile Glu Ile Gly Glu Thr Glu Gly Thr Phe

1145 1150 1155

Ile Val Asp Ser Val Glu Leu Leu Leu Met Glu Glu

1160 1165 1170

Claims

1.一种嵌合杀昆虫蛋白质，其氨基酸序列如SEQ ID NO:10所示。

2.一种编码嵌合杀昆虫蛋白质的多核苷酸，其中所述多核苷酸可操作地连接至异源启动子，并且所述嵌合杀昆虫蛋白质的氨基酸序列如SEQ ID NO:10所示。

3.一种编码嵌合杀昆虫蛋白质的多核苷酸，其中所述多核苷酸编码氨基酸序列如SEQID NO:10所示的嵌合杀昆虫蛋白质。

4.一种宿主细胞，其包含编码嵌合杀昆虫蛋白质的多核苷酸，其中所述嵌合杀昆虫蛋白质的氨基酸序列如SEQ ID NO:10所示或其中所述多核苷酸序列如SEQ ID NO:9所示，其中所述宿主细胞是选自由细菌宿主细胞和不可再生的植物宿主细胞组成的群组。

5.如权利要求4所述的宿主细胞，其中所述细菌宿主细胞是选自由以下各项组成的群组：土壤杆菌、根瘤菌、芽孢杆菌、短芽孢杆菌、埃希氏杆菌、假单胞菌、克雷伯氏杆菌和欧文氏菌。

6.如权利要求4所述的宿主细胞，其中所述宿主细胞是源自选自由单子叶植物和双子叶植物组成的植物群组的植物的不可再生的植物宿主细胞。

7.一种昆虫抑制性组合物，其包含氨基酸序列如SEQ ID NO:10所示的嵌合杀昆虫蛋白质。

8.如权利要求7所述的昆虫抑制性组合物，其还包含与所述嵌合杀昆虫蛋白质不同的至少一种昆虫抑制剂。

9.如权利要求8所述的昆虫抑制性组合物，其中所述至少一种昆虫抑制剂是选自由以下各项组成的群组：昆虫抑制性蛋白质、昆虫抑制性dsRNA分子和昆虫抑制性化学物质。

10.如权利要求8所述的昆虫抑制性组合物，其中所述至少一种昆虫抑制剂对鳞翅目、鞘翅目、半翅目、同翅目或缨翅目的一个或多个害虫物种表现出活性。

11.一种防治鳞翅目害虫的方法，所述方法包括使所述鳞翅目害虫与抑制量的如权利要求1所述的嵌合杀昆虫蛋白质接触。

12.一种不可再生的转基因植物细胞，其包含嵌合杀昆虫蛋白质，其中：

所述嵌合杀昆虫蛋白质的氨基酸序列如SEQ ID NO:10所示。

13.一种防治鳞翅目害虫的方法，其包括使所述害虫暴露于如权利要求12所述的不可再生的转基因植物细胞，其中所述不可再生的植物细胞表达鳞翅目抑制量的所述嵌合杀昆虫蛋白质。

14.一种来源于如权利要求12所述的不可再生的植物细胞的商品产品，其中所述产品包含可检测量的所述嵌合杀昆虫蛋白质。

15.如权利要求14所述的商品产品，其中所述产品是选自由以下各项组成的群组：油、膳食、动物饲料、面粉、薄片、糠、棉绒和外壳。

16.一种编码如权利要求1所述的嵌合杀昆虫蛋白质的重组多核苷酸分子，其包含编码嵌合杀昆虫蛋白质的多核苷酸，其中所述嵌合杀昆虫蛋白质的氨基酸序列如SEQ ID NO:10所示或其中所述多核苷酸序列如SEQ ID NO:9所示；和编码与所述嵌合杀昆虫蛋白质不同的昆虫抑制剂的多核苷酸序列。

17.一种重组核酸分子，其包含可操作地连接至编码嵌合杀昆虫蛋白质的多核苷酸片段的异源启动子，其中：

所述嵌合杀昆虫蛋白质的氨基酸序列如SEQ ID NO:10所示。