CN111920733A

CN111920733A - Mite-removing conditioner, facial cleanser and preparation method of mite-removing conditioner

Info

Publication number: CN111920733A
Application number: CN202010814880.1A
Authority: CN
Inventors: 龚德明; 容惠; 徐洁琼; 肖雨虹
Original assignee: Guangzhou Guangzhuang Biotechnology Co ltd
Current assignee: Guangzhou Jinan Cosmetics Co ltd
Priority date: 2020-08-13
Filing date: 2020-08-13
Publication date: 2020-11-13
Anticipated expiration: 2040-08-13
Also published as: CN111920733B

Abstract

The invention provides a mite-killing conditioner, a facial cleanser and a preparation method thereof. The mite-killing conditioner comprises: a mite-killing plant composition, oat beta-glucan, a hydrolyzed yeast extract, a snow lotus herb extract, an aloe extract and tea tree essential oil; the mite-killing plant composition comprises: rosewood seed extract, zanthoxylum bungeanum fruit extract, sophora flavescens root extract, stemona japonica root extract and phellodendron amurense bark extract. The mite-killing conditioner can also inhibit oil secretion radically by reducing the activity of sebaceous glands on the basis of high-efficiency mite killing. Meanwhile, the mite removing plant composition can promote the exertion of other functional components of the conditioner, so that the effects of pore astringency, antibiosis, anti-inflammation, moisture retention and water replenishing are further improved.

Description

Mite-removing conditioner, facial cleanser and preparation method of mite-removing conditioner

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to a mite-removing conditioner, a facial cleanser and a preparation method thereof.

Background

In recent years, mites are found to be closely related to human health, and mites parasitic on human bodies are mainly divided into two types, namely follicle mites and sebaceous gland mites, which are parasitic in sebaceous glands and hair follicles of human faces. When a few people sweat when infected with mites on the face and feel slight itching on the nose and the face when sleeping at night, blackheads appear after a period of time because secretion excreted by the mites blocks pores and is dried and hardened, the pores gradually become thick, the skin begins to change from neutrality to mixedness and then becomes oily, and skin inflammation can be caused if effective treatment is not timely obtained.

Although some mite killing articles exist in the market at present, the effect is not ideal, and the patients cannot completely get rid of the trouble of mites. These mite killing articles basically have four problems: firstly, the mite removing effect is not obvious, the speed is low, old mites are not removed completely, new mites grow out again, the circulation is repeated, and the mite removing is remote and has no period; secondly, the ingredients have complex sources and low safety, and are easy to cause subacute percutaneous toxicity when being applied to remove mites on the skin; thirdly, the understanding of the growing environment of the mites is lacked, and only the mites are removed without inhibiting the mites, so that the problem of the mites is repeated; fourth, in terms of product formulation, the design is rarely made according to the skin characteristics of men, and the using effect of most men is poor.

CN102335109A discloses a plant sterilizing and mite removing facial cleanser, which comprises the following components: 15-45 parts of acyl sodium sulfonate, 0.2-20 parts of octadecanoic acid, 2-30 parts of xylitol, 5-15 parts of palm wax, 5-15 parts of sodium carboxymethylcellulose, 5-20 parts of vitamin E and 800 parts of plant concentrated solution. The plant concentrated solution comprises the following raw materials in percentage by weight: 10% of Chinese gall, 10% of fructus kochiae, 10% of rheum officinale, 25% of geranium and 45% of fructus forsythiae. The plant sterilization mite-removal facial cleanser relies on the addition of the high-content plant concentrated solution to remove mites, however, the high-content plant concentrated solution easily causes subacute percutaneous toxicity, and the plant sterilization mite-removal facial cleanser cannot inhibit grease secretion from roots, so that the problem of mite regeneration is difficult to solve.

CN110420266A discloses a three-pepper acne-removing mite-removing agent which mainly contains a zanthoxylum bungeanum maxim extract, a capsicum frutescens maxim extract and a kava root extract. The three-pepper acne-removing composition disclosed by the invention has a certain mite removing effect, the mite removing effect is still to be improved, and the composition has weak capability of reducing the activity of sebaceous glands and inhibiting grease secretion, so that the aim of inhibiting the reproduction of mites cannot be achieved from a food chain.

Therefore, the development of a male skin care product which can effectively remove mites and has the functions of oil control, moisture retention, inflammation resistance and bacteriostasis is the focus of the current research.

Disclosure of Invention

The invention aims to provide a mite-killing conditioner, a facial cleanser and a preparation method thereof. The mite-killing conditioner can also inhibit oil secretion radically by reducing the activity of sebaceous glands on the basis of high-efficiency mite killing. Meanwhile, the mite removing plant composition can promote the exertion of other functional components of the conditioner, so that the effects of pore astringency, antibiosis, anti-inflammation, moisture retention and water replenishing are further improved.

In order to achieve the purpose, the invention adopts the following technical scheme:

in a first aspect, the present invention provides a mite control conditioner comprising: a mite-killing plant composition, oat beta-glucan, a hydrolyzed yeast extract, a snow lotus herb extract, an aloe extract and tea tree essential oil;

the mite-killing plant composition comprises: rosewood seed extract, zanthoxylum bungeanum fruit extract, sophora flavescens root extract, stemona japonica root extract and phellodendron amurense bark extract.

In the invention, the active ingredients in the mite removing plant composition are mutually matched, so that the synergistic effect is realized, and the mite removing plant composition can interfere the permeability of the cell membrane of the mites and block the absorption of the cell membrane to nutrient substances, thereby achieving the mite inhibiting effect; and the possibility of inhibiting the reproduction of mites from a food chain is achieved by reducing the activity of pores, inhibiting the secretion of sebum, reducing the activity of sebaceous glands and radically inhibiting the secretion of grease.

In the invention, the active ingredients of the mite-removing plant composition, the oat beta-glucan, the hydrolyzed yeast extract, the snow lotus extract, the aloe extract and the tea tree essential oil are matched with each other, so that a synergistic effect is achieved, the mite inhibiting and oil controlling effects of the mite-removing plant composition can be further improved, the mite-removing plant composition can promote the exertion of other effective components of a conditioner, and the effects of pore astringing, antibiosis, anti-inflammation, moisture retention and water replenishing are further improved.

The oat beta-glucan is a non-starch polysaccharide existing in cell walls of oat endosperm and aleurone layer, has obvious anti-inflammatory and antiallergic activities, can help skin to resist various exogenous mechanical and chemical stimuli, can help to refresh and repair tense skin, and improves the moisture retention property and the firmness and smoothness of the skin. The hydrolyzed yeast extract is obtained by autolysis or by hydrolysis with an added enzyme. The hydrolytic yeast extract is rich in nutrition, amino acid, small peptide, B vitamins, glutathione and nucleic acid substances, has the effects of resisting oxidation and reducing oil and fat when being added into cosmetics, and can also supplement nutrition for skin.

Wherein, the content of flavonoid substances in the snow lotus flower extract is high, and the reducing power is strong. The saussurea involucrate extract has good dose-effect relationship and linear relationship when removing hydroxyl free radicals, superoxide anion free radicals and DPPH free radicals, wherein the capacity of removing superoxide anion free radicals and DPPH free radicals is strong, and the effects of resisting oxidation and preserving moisture are obvious. The aloe extract retains the rich nutrition of fresh aloe, has super strong anti-inflammatory ability, can eliminate sensitive bacteria and fungi on the skin surface, and can prevent dermatitis from breeding. The tea tree essential oil is easy to permeate into skin to dredge pores, and can sterilize and disinfect the pores, so that inflammation is fully controlled.

Preferably, the mite removing conditioner comprises the following components in parts by weight: 3-10 parts of mite-killing plant composition, 2-8 parts of oat beta-glucan, 2-8 parts of hydrolytic yeast extract, 1-5 parts of snow lotus herb extract, 1-5 parts of aloe extract and 1-5 parts of tea tree essential oil.

In the mite-killing conditioner, the content of the mite-killing plant composition is 3 to 10 parts, and can be 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts, 10 parts and the like.

In the mite-killing conditioner, the content of the oat beta-glucan is 2-8 parts, and can be 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts and the like.

In the mite-killing conditioner, the content of the hydrolyzed yeast extract is 2 to 8 parts, and may be, for example, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, or the like.

In the mite-killing conditioner, the content of the snow lotus herb extract is 1-5 parts, such as 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts and the like.

In the mite-killing conditioner, the content of the aloe extract is 1-5 parts, and can be 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts and the like.

In the mite-removing conditioner, the content of the tea tree essential oil is 1-5 parts, and can be 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts and the like.

Preferably, the mite removing plant composition comprises the following components in parts by weight: 5-10 parts of rosewood seed extract, 5-10 parts of zanthoxylum bungeanum fruit extract, 5-10 parts of sophora flavescens root extract, 1-5 parts of radix stemonae petiolata root extract and 1-5 parts of phellodendron bark extract.

In the mite removing plant composition, the content of the rosewood seed extract is 5-10 parts, such as 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts and the like.

In the mite removing plant composition, the content of the zanthoxylum fruit extract is 5-10 parts, for example, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts and the like.

In the mite-killing plant composition, the content of the sophora flavescens root extract is 5-10 parts, such as 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts and the like.

In the mite removing plant composition, the content of the stemona tuberosa root extract is 1-5 parts, and can be 1 part, 2 parts, 3 parts, 4 parts, 5 parts and the like, for example.

In the mite-killing plant composition, the content of the phellodendron amurense bark extract is 1-5 parts, and can be 1 part, 2 parts, 3 parts, 4 parts, 5 parts and the like, for example.

In a second aspect, the invention provides a facial cleanser, which is prepared from the following raw materials: a mite-killing conditioner as described in the first aspect, a surfactant, a thickener, a pH regulator and water.

Preferably, the preparation raw materials of the facial cleanser comprise the following components in percentage by mass: 0.1-2% of mite-killing conditioner, 20-50% of surfactant, 0.5-2% of thickening agent, 5-6% of pH regulator and the balance of water.

The amount of the mite-removing conditioner added in the first aspect is 0.1 to 2%, for example, 0.1%, 0.2%, 0.4%, 0.6%, 0.8%, 0.9%, 1%, 1.2%, 1.4%, 1.6%, 1.8%, 2% or the like, based on 100% by mass of the raw materials for preparing the face cleanser.

The amount of the surfactant added is 20 to 50% based on 100% by mass of the raw materials for producing the face cleanser, and may be, for example, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 28%, 30%, 32%, 33%, 34%, 36%, 37%, 38%, 40%, 45%, 50% or the like.

The amount of the thickener added is 0.5 to 2%, for example, 0.5%, 0.6%, 0.8%, 1.2%, 1.4%, 1.6%, 1.8%, 2%, etc., based on 100% by mass of the raw materials for producing the face cleanser.

The amount of the pH adjuster added is 5 to 6%, for example, 5%, 5.1%, 5.2%, 5.3%, 5.4%, 5.5%, 5.6%, 5.8%, 6%, etc., based on 100% by mass of the starting material for producing the face cleanser.

Preferably, the surfactant comprises any one or a combination of at least two of stearic acid, myristic acid, palmitic acid, lauric acid, monoglyceride, tween 60, sodium fatty alcohol polyoxyethylene ether sulphate or coconut oil fatty acid diethanolamide, preferably a combination of stearic acid, myristic acid, palmitic acid, lauric acid, monoglyceride, tween 60, sodium fatty alcohol polyoxyethylene ether sulphate and coconut oil fatty acid diethanolamide.

According to the preferable technical scheme, the surfactant is a combination of octadecanoic acid, tetradecanoic acid, hexadecanoic acid, dodecanoic acid, monoglyceride, tween 60, sodium fatty alcohol polyoxyethylene ether sulfate and coconut oil fatty acid diethanolamide, all the components are matched with each other, the synergistic effect is achieved, and by utilizing the inherent physical and chemical phenomena of the soap-based surfactant, the soap-based product is enabled to have excellent moistening feeling and excellent foam stability at the same time, and the absorption of the mite-killing conditioner can be promoted.

Preferably, the thickener comprises polyethylene glycol distearate and/or sodium hydroxyethyl cellulose, preferably a combination of polyethylene glycol distearate and sodium hydroxyethyl cellulose.

Preferably, the pH adjuster is potassium hydroxide.

Preferably, the raw materials for preparing the facial cleanser further comprise any one or a combination of at least two of a humectant, a preservative or an aromatic.

Preferably, the raw materials for preparing the facial cleanser also comprise 5-8% of a humectant, for example, 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8% and the like, based on 100% of the raw materials for preparing the facial cleanser.

Preferably, the humectant is glycerin.

Preferably, the raw materials for preparing the facial cleanser further comprise 0.1-1% of preservative, for example, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1% and the like, based on 100% of the raw materials for preparing the facial cleanser.

Preferably, the preservative is any one or a combination of at least two of methyl hydroxybenzoate, propyl hydroxybenzoate or phenoxyethanol.

Preferably, the raw materials for preparing the facial cleanser further comprise 0.01-0.3% of an aromatic, for example, 0.01%, 0.02%, 0.04%, 0.06%, 0.08%, 0.1%, 0.15%, 0.2%, 0.25%, 0.3%, etc., based on 100% of the raw materials for preparing the facial cleanser.

Preferably, the fragrance is a perfume.

In a third aspect, the present invention provides a method for preparing the facial cleanser according to the second aspect, wherein the method for preparing the facial cleanser comprises the following steps:

(1) mixing a thickening agent, a pH regulator and water to obtain a water phase mixed solution;

(2) mixing the first mixed solution obtained in the step (1) with a surfactant to obtain a second mixed solution;

(3) and (3) mixing the second mixed solution obtained in the step (2) with the mite removing conditioner, and filtering to obtain the facial cleanser.

Preferably, the temperature of the mixing in step (1) is 80-85 deg.C, such as 80 deg.C, 81 deg.C, 82 deg.C, 83 deg.C, 84 deg.C, 85 deg.C, etc., and the mixing time is 10-20min, such as 10min, 12min, 14min, 16min, 18min, 20min, etc.

Preferably, the temperature of the mixing in the step (2) is 80-85 ℃, for example 80 ℃, 81 ℃, 82 ℃, 83 ℃, 84 ℃, 85 ℃ and the like, and the mixing time is 60-90min, for example 60min, 65min, 70min, 75min, 80min, 85min, 90min and the like.

Preferably, the mixing temperature in step (3) is 40-50 deg.C, such as 40 deg.C, 42 deg.C, 44 deg.C, 46 deg.C, 48 deg.C, 50 deg.C, etc., and the mixing time is 5-15min, such as 5min, 7min, 9min, 11min, 13min, 15min, etc.

Preferably, the filtration in step (3) is performed by using 100-300 mesh filter cloth, for example, 100 mesh, 120 mesh, 140 mesh, 160 mesh, 180 mesh, 200 mesh, 220 mesh, 240 mesh, 260 mesh, 280 mesh, 300 mesh and the like.

Preferably, the preparation method of the facial cleanser comprises the following steps:

(1) mixing the thickening agent, the pH regulator and water at 80-85 ℃ for 10-20min to obtain a first mixed solution;

(2) mixing the first mixed solution obtained in the step (1) with a surfactant at 80-85 ℃ for 60-90min to obtain a second mixed solution;

(3) and (3) mixing the second mixed solution obtained in the step (2), the mite removing conditioner, the humectant, the preservative and the aromatic at 40-50 ℃ for 5-15min, and filtering by using 100-mesh and 300-mesh filter cloth to obtain the facial cleanser.

Compared with the prior art, the invention has the following beneficial effects:

(1) the mite-killing conditioner can kill mites efficiently, inhibit grease secretion radically by reducing the activity of sebaceous glands, and inhibit the absorption of cell membranes to nutrient substances by interfering the permeability of the cell membranes of the mites, so that the mite-killing effect is achieved; meanwhile, the mite removing plant composition can promote the exertion of other functional components in the conditioner, so that the effects of moisturizing, resisting bacteria and resisting inflammation are further improved;

(2) the insecticidal rate of the facial cleanser prepared by adding the mite removing conditioner is more than 97% in a mite removing test; the clearance rate of skin oil in 8 hours after washing is more than 40%; the moisture retention rate is more than 80% after 4 hours; after 4h, the moisture content of the skin is more than 45%; the change rate of percutaneous water loss is maintained below 30 percent after continuous use for 4 days; the inhibition rate on PGE2 release is more than 80%, and the inhibition rate on TNF-a release is more than 80%; the diameter of the inhibition zone for staphylococcus aureus is 19-24mm, and the diameter of the inhibition zone for propionibacterium acnes is 16-22 mm.

Detailed Description

The technical solution of the present invention is further explained by the following embodiments. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention.

Preparation example 1

The preparation example provides a rosewood seed extract, which is prepared by the following preparation method:

(1) crushing rosewood seeds to a particle size of 50 meshes, mixing the rosewood seeds with 70 wt% of ethanol water solution in a mass ratio of 1:10, and performing microwave extraction for 5min, wherein the microwave extraction power is 1kW, and the microwave extraction frequency is 3 times to obtain a rosewood seed extracting solution;

(2) centrifuging the rosewood seed extracting solution obtained in the step (1) at the rotating speed of 7000r/min by adopting a disc centrifuge, and concentrating by adopting a reverse osmosis membrane, wherein the concentration pressure is 10bar to obtain a crude rosewood seed extract;

(3) mixing the crude extract of the rosewood seeds obtained in the step (2) with maltodextrin in a mass ratio of 4:6, performing ultrasonic treatment for 60min at the ultrasonic power of 300W, and freezing at-15 ℃ to obtain the rosewood seed extract.

Preparation example 2

The preparation example provides a zanthoxylum bungeanum maxim fruit extract, which is prepared by the following preparation method: crushing dry pricklyash peel to 150 meshes, mixing the crushed dry pricklyash peel with petroleum ether, standing and extracting, mixing the mixture with acetone, standing and extracting, and filtering to obtain filtrate, wherein the mass ratio of the dry pricklyash peel to the petroleum ether is 1:2, and the mass ratio of the dry pricklyash peel to the acetone is 1: 3; mixing the obtained filtrate with a 2 wt% sodium bicarbonate solution, stirring, mixing with a saturated saline solution, standing for layering, collecting an upper layer solution, and performing rotary steaming and concentration to obtain the zanthoxylum bungeanum fruit extract, wherein the mass ratio of the dry zanthoxylum bungeanum fruit to the 2 wt% sodium bicarbonate solution is 1:80, and the mass ratio of the dry zanthoxylum bungeanum fruit to the saturated saline solution is 1: 80.

Preparation example 3

The preparation example provides a sophora flavescens root extract, which is prepared by the following preparation method: pulverizing radix Sophorae Flavescentis into 150 mesh, mixing with 75 wt% ethanol water solution at a mass ratio of 1:10, reflux extracting for 3 times, mixing extractive solutions, rotary steaming, concentrating, and spray drying at 40 deg.C and 30MPa to obtain radix Sophorae Flavescentis extract.

Preparation example 4

The preparation example provides a stemona tuberosa root extract, which is prepared by the following preparation method:

(a) pulverizing radix Stemonae to 150 mesh, mixing with water at a mass ratio of 1:10, standing and extracting to obtain water extract;

(b) microfiltration is carried out on the water extraction product obtained in the step (a), the microfiltration is carried out by adopting a ceramic membrane, the number of pores of the ceramic membrane is 35, the separation precision of the ceramic membrane is 80nm, then ultrafiltration is carried out, and the molecular weight of the interception pore diameter of ultrafiltration membrane separation equipment is larger than 1000, so that filter residue and filtrate are obtained;

(c) mixing the filter residue obtained in the step (b) with ethanol for alcohol precipitation to obtain an extract I, concentrating the filtrate obtained in the step (b), performing spray drying at 40 ℃ and under 30MPa to obtain an extract II, and combining the extract I and the extract II to obtain the stemona tuberosa root extract.

Preparation example 5

The preparation example provides a phellodendron amurense bark extract, which is prepared by the following preparation method:

(A) pulverizing cortex Phellodendri bark to 150 mesh, mixing with n-hexane at a mass ratio of 1:2, soaking at 30 deg.C for 3 hr, filtering, and drying to obtain defatted cortex Phellodendri bark powder;

(B) mixing the degreased phellodendron amurense bark powder obtained in the step (A) with 60 wt% ethanol water solution according to the mass ratio of 1:10, performing ultrasonic extraction for 60min at the power of 200W, and concentrating to obtain a crude phellodendron amurense bark extract;

(C) dissolving the coarse extract of the phellodendron amurense bark obtained in the step (B) by using 10 wt% of ethanol water solution, purifying by using a D4020 macroporous resin gel column, eluting by using 60 wt% of ethanol water solution, and concentrating to obtain the phellodendron amurense bark extract.

Preparation example 6

The preparation example provides a mite removing plant composition I, which is prepared by the following preparation method: mixing and stirring 8 parts of rosewood seed extract, 6 parts of zanthoxylum bungeanum fruit extract, 6 parts of sophora flavescens root extract, 2 parts of stemona tuberosa root extract and 4 parts of phellodendron amurense bark extract at 25 ℃ for 10min to obtain the mite removing plant composition I.

Preparation example 7

The preparation example provides a mite removing plant composition II, which is prepared by the following preparation method: mixing and stirring 6 parts of rosewood seed extract, 5 parts of zanthoxylum bungeanum fruit extract, 9 parts of sophora flavescens root extract, 4 parts of stemona tuberosa root extract and 2 parts of phellodendron amurense bark extract at 25 ℃ for 10min to obtain the mite removing plant composition II.

Preparation example 8

The preparation example provides a mite removing plant composition III, which is prepared by the following preparation method: mixing 7 parts of rosewood seed extract, 8 parts of zanthoxylum bungeanum fruit extract, 5 parts of sophora flavescens root extract, 3 parts of stemona tuberosa root extract and 3 parts of phellodendron amurense bark extract at 25 ℃, and stirring for 10min to obtain the mite removing plant composition III.

The mite-killing plant compositions in the following examples and comparative examples were prepared by the above-mentioned preparation method, and other components were commercially available.

Example 1

The embodiment provides a mite removing conditioner, which comprises the following components in parts by weight: 5 parts of mite-killing plant composition I, 5 parts of oat beta-glucan, 5 parts of hydrolytic yeast extract, 4 parts of snow lotus herb extract, 3 parts of aloe extract and 3 parts of tea tree essential oil, wherein the parts are provided in preparation example 6.

Example 2

The embodiment provides a mite removing conditioner, which comprises the following components in parts by weight: 6 parts of mite-killing plant composition II, 3 parts of oat beta-glucan, 6 parts of hydrolytic yeast extract, 2 parts of snow lotus herb extract, 4 parts of aloe extract and 4 parts of tea tree essential oil, wherein the parts are provided in preparation example 7.

Example 3

The embodiment provides a mite removing conditioner, which comprises the following components in parts by weight: the mite-killing plant composition III provided by the preparation example 8 comprises 7 parts of oat beta-glucan, 4 parts of hydrolytic yeast extract, 3 parts of snow lotus herb extract, 2 parts of aloe extract and 3 parts of tea tree essential oil.

Example 4

The embodiment provides a mite removing conditioner, which comprises the following components in parts by weight: the mite-killing plant composition I provided by the preparation example 6 comprises 2 parts of oat beta-glucan, 6 parts of hydrolytic yeast extract, 4 parts of snow lotus herb extract, 4 parts of aloe extract and 3 parts of tea tree essential oil.

Example 5

The embodiment provides a mite removing conditioner, which comprises the following components in parts by weight: the mite-killing plant composition I12 parts, 1 part of oat beta-glucan, 1 part of hydrolytic yeast extract, 4 parts of snow lotus herb extract, 4 parts of aloe extract and 3 parts of tea tree essential oil provided by preparation example 6.

Comparative examples 1 to 6

Comparative examples 1-6 each provide different mite control conditioners having the formula in parts by weight shown in table 1 below:

TABLE 1

Application example 1

The application embodiment provides a facial cleanser, which comprises the following raw materials in percentage by mass:

the preparation method of the facial cleanser comprises the following steps:

(1) mixing a thickening agent, a pH regulator and water at 82 ℃ for 15min to obtain a first mixed solution;

(2) mixing the first mixed solution obtained in the step (1) with a surfactant at 82 ℃ for 80min to obtain a second mixed solution;

(3) and (3) mixing the second mixed solution obtained in the step (2), the mite removing conditioner, the humectant, the preservative and the aromatic at 45 ℃ for 10min, and filtering by adopting filter cloth of 200 meshes to obtain the facial cleanser.

Application example 2

The application example provides a facial cleanser, which is different from the facial cleanser in the application example 1 in that the mite removing conditioner provided in the example 1 is replaced by the mite removing conditioner provided in the example 2, and the contents of other components and the preparation method are the same as those in the application example 1.

Application example 3

The application example provides a facial cleanser, which is different from the facial cleanser in the application example 1 in that the mite removing conditioner provided in the example 1 is replaced by the mite removing conditioner provided in the example 3, and the contents of other components and the preparation method are the same as those in the application example 1.

Application example 4

The application example provides a facial cleanser, which is different from the facial cleanser in the application example 1 in that the mite removing conditioner provided in the example 1 is replaced by the mite removing conditioner provided in the example 4, and the contents of other components and the preparation method are the same as those in the application example 1.

Application example 5

The application example provides a facial cleanser, which is different from the facial cleanser in the application example 1 in that the mite removing conditioner provided in the example 1 is replaced by the mite removing conditioner provided in the example 5, and the contents of other components and the preparation method are the same as those in the application example 1.

Application example 6

The application example provides a facial cleanser, which is different from the application example 1 in that octadecanoic acid is not added into the surfactant, the content of tetradecanoic acid is increased to 11%, the content of hexadecanoic acid is increased to 10%, the content of dodecanoic acid is increased to 13%, and the content of other components and the preparation method are the same as those in the example 1.

Application example 7

The application example provides a facial cleanser, which is different from the application example 1 in that myristic acid is not added into the surfactant, the content of stearic acid is increased to 17%, the content of palmitic acid is increased to 7%, the content of lauric acid is increased to 10%, and the content of other components and the preparation method are the same as those in the example 1.

Application example 8

The application example provides a facial cleanser, which is different from the application example 1 in that hexadecanoic acid is not added into the surfactant, the content of octadecanoic acid is increased to 17%, the content of tetradecanoic acid is increased to 8%, the content of dodecanoic acid is increased to 9%, and the content of other components and the preparation method are the same as those in the example 1.

Application example 9

The application example provides a facial cleanser, which is different from the application example 1 in that dodecanoic acid is not added into the surfactant, the content of octadecanoic acid is increased to 18%, the content of tetradecanoic acid is increased to 9%, the content of hexadecanoic acid is increased to 7%, and the content of other components and the preparation method are the same as those in the example 1.

Application example 10

The application example provides a facial cleanser, and the difference from the application example 1 is that no monoglyceride is added into the surfactant, the content of tween 60 is increased to 1.8%, and the content of other components and the preparation method are the same as those in the example 1.

Application example 11

The application example provides a facial cleanser, and the difference from the application example 1 is that no tween 60 is added into the surfactant, the content of monoglyceride is increased to 1.8%, and the content of other components and the preparation method are the same as those in the example 1.

Application example 12

The application example provides a facial cleanser, and the difference from the application example 1 is that no fatty alcohol-polyoxyethylene ether sodium sulfate is added into the surfactant, the content of coconut oil fatty acid diethanolamide is increased to 5%, and the content of other components and the preparation method are the same as those in the example 1.

Application example 13

The application example provides a facial cleanser, and the difference from the application example 1 is that coconut oil fatty acid diethanolamide is not added into the surfactant, the content of the sodium fatty alcohol polyoxyethylene ether sulfate is increased to 5%, and the content of other components and the preparation method are the same as those in the example 1.

Application of comparative examples 1 to 6

Compared with the application example 1, the mite removing conditioner provided by the example 1 is respectively and correspondingly replaced by the mite removing conditioner provided by the comparative examples 1-6, and the content of other components and the preparation method are the same as the application example 1.

Application comparative example 7

Compared with the application example 1, the facial cleanser is not added with the mite removing conditioner, the shortage is supplemented to 100% by water, and the contents of other components and the preparation method are the same as those of the application example 1.

Test example 1

Safety performance testing

The safety performance tests were performed on the face cleansers provided in application examples 1 to 13 and the face cleansers provided in application comparative examples 1 to 7 by the following methods:

(1) haemolysis test of erythrocytes

Preparation of erythrocyte suspension: selecting healthy rabbits, taking 9mL of blood from heart, adding 1mL of 2% potassium oxalate solution, centrifuging, discarding supernatant, diluting the precipitate to 20mL with 20mmol/L PBS solution, and storing at 4 ℃ for later use. Select samples were diluted with PBS solution to different concentrations, with 5 concentration gradients set for each sample. Adding 200 μ L of the above erythrocyte suspension (final concentration of the sample is controlled to be 5, 10, 20, 50, 100mg/mL respectively) into 10mL of diluent of the sample to be tested, taking distilled water as total blood-dissolving control, taking PBS solution as negative control, mixing gently, incubating at 37 deg.C for 30min, centrifuging at 2000r/min for 10min, collecting supernatant, and testing at 560nm with spectrophotometerAbsorbance (A) of (A)₅₆₀) Calculating the hemolysis rate according to the following formula;

a standard curve of hemolysis rate vs. sample concentration was plotted, and the sample concentration at which hemolysis occurred in 50% erythrocytes (HD) was calculated₅₀)。

(2) Protein denaturation experiments:

diluting the sample to 10g/L with PBS solution, collecting 10mL dilution of the sample to be tested, adding 200 μ L of the erythrocyte suspension, using distilled water as blank control, 1mg/mL Sodium Dodecyl Sulfate (SDS) solution as positive control, mixing gently, incubating at 37 deg.C for 30min, centrifuging at 2000r/min for 10min, collecting supernatant, and testing absorbance A at 540nm and 575nm with spectrophotometer₅₄₀And A₅₇₅Calculating a protein Denaturation Index (DI) according to the following formula;

wherein R is₁Blank control group a₅₇₅Blank control group A₅₄₀，R₂Experimental group a₅₇₅Experimental group A₅₄₀，R₃Positive control group A₅₇₅Positive control group A₅₄₀。

Evaluating the irritation of the sample to be tested according to the L/D value, wherein the L/D value is HD₅₀DI, erythrocyte hemolysis assay irritation grading criteria are shown in Table 2 below:

TABLE 2

The results of the above-described hemolysis test and protein denaturation test are shown in Table 3 below:

TABLE 3

As can be seen from the safety performance test, the facial cleansers prepared according to the application examples 1 to 13 of the present invention are mild and non-irritating; the sample concentration HD of the facial cleanser prepared by the invention when 50% of red blood cells are hemolyzed₅₀HD higher than that of facial cleanser prepared by using comparative examples 1-7 and more than 11000mg/L₅₀(ii) a Meanwhile, the protein denaturation index DI is below 5 percent and is obviously smaller than the facial cleanser prepared by the application comparative examples 1-7, which shows that the addition of the mite-removing conditioner can obviously reduce the toxic and side effects and the irritation of the facial cleanser and is safer and more reliable.

Test example 2

Mite killing rate test

(1) Acquisition of human demodex: adopting a transparent adhesive tape sticking method to obtain the demodex, and specifically comprising the following steps: the examinee is ordered to wash face before sleeping, and then apply transparent adhesive tapes with length of 6cm and width of 1.5cm to the forehead, cheek, nose, chin, etc., and then take off the transparent adhesive tapes and stick the transparent adhesive tapes to the adhesive tapes next morning, and then carry out microscopic examination and seal the transparent adhesive tapes on a glass slide.

(2) Grouping tests: each glass slide is dripped with 0.1mg/cm²The cleansing milk provided in application examples 1 to 13 and the cleansing milk provided in application comparative examples 1 to 7 spread the cleansing milk uniformly to ensure that the cleansing milk is in full contact with the worm bodies. Placing in an environment with relative humidity of 70-80% and temperature of 26-28 deg.C, taking out after 24 hr, and observing demodex survival condition under microscope. After continuously observing under a 400X microscope for 3min, the dead insect is judged to be dead by the dead insect.

The specific test results are shown in table 4 below:

TABLE 4

The test results in table 4 show that the insecticidal rate in the facial cleanser mite removal test prepared by adding the mite removal conditioner of the invention is more than 97%, which shows that the mite removal plant composition, the oat beta-glucan, the hydrolyzed yeast extract, the snow lotus flower extract, the aloe extract and the tea tree essential oil in the mite removal conditioner of the invention are mutually matched, so that the mite removal conditioner has a synergistic effect, can effectively remove mites, and can inhibit grease secretion by reducing the activity of sebaceous glands and root sources, and can inhibit the absorption of nutrient substances by cell membranes by interfering the permeability of the cell membranes of mites, thereby achieving the mite inhibition effect.

Test example 3

Oil clearance test

(1) 100 volunteers with oily skin, 25-50 years old, were selected and randomly divided into 20 groups of 5 persons each. Volunteers could not use any oil control product, such as cosmetics, external drugs or health products for internal use, 30 days before the start of the experiment. Before the test, the test piece enters a constant-temperature and constant-humidity room with the temperature of 22 +/-2 ℃ and the humidity of 50 +/-5 percent and sits statically for 30min, the left forehead and the right forehead of a volunteer are randomly selected, the intersection point of the midline between the eyebrows and the parallel line 1cm away from the upper eyebrow edge is taken as a test middle point, then the test positions are respectively arranged in parallel from left to right at the test middle point, the average value of 3 values is taken as the grease data of 0h on the forehead, then each group of test pieces are required to respectively use the facial cleanser provided in the application examples 1-13 and the facial cleanser provided in the application examples 1-7 on the single side of the left cheek and the right cheek for 15min for cleaning, and the grease test is carried out. Calculating the clearance rate of skin oil in each time period;

(2) number of Active Pores (NAP) test: 100 male men of 25-50 years old with oily skin are selected and randomly divided into 20 groups of 5 persons, and each group is respectively smeared with a proper amount of each test sample on the face and is used once every morning and evening. The Number of Active Pores (NAP) at week 4 was tested using an active skin surface analysis system, and the average of the user test results was taken, and the reduction in the number of active pores and the reduction in the percentage of sebum on the skin surface were recorded with the initial value of the Number of Active Pores (NAP) 30min after the test area was cleaned.

The specific test results are shown in fig. 5:

TABLE 5

The test results in table 4 show that the clearance rate of skin oil after 8 hours of the cleansing whey prepared by adding the mite removal conditioner disclosed by the invention is over 40%; the reduction degree of the active pore quantity is-42% to-34%. The mite-killing conditioner can effectively kill mites, inhibit sebum secretion, regulate water-oil balance of skin, reduce sebaceous gland activity and achieve the possibility of inhibiting the reproduction of mites from a food chain.

Test example 4

Test for moisture retention

The moisturizing performance of the face cleansers provided in application examples 1 to 13 and the face cleansers provided in application comparative examples 1 to 7 were tested as follows:

(1) in vitro weighing method for testing moisture retention performance

Weighing 0.2g of each sample to be tested, respectively and uniformly coating the sample on a 5cm multiplied by 5cm glass plate stuck with the microporous ventilating adhesive tape, putting the glass plate into a dryer with constant temperature and constant humidity, respectively weighing the mass of the glass plate after being placed for 4 hours, and calculating the moisture retention rate. The moisture retention rate calculation formula is as follows:

moisture retention rate/% (M)₂-M₀)/(M₁-M₀)×100％。

Wherein M is₀Is the mass/g, M, of the glass sheet₁For the mass/g, M of the glass plate after sample application₂The glass sheet mass/g after standing in the desiccator for several hours.

(2) Rate of change of percutaneous water loss

100 male persons aged 25 to 50 years who had oily skin were selected and randomly divided into 20 groups of 5 persons, and the facial cleansers provided in application examples 1 to 13 and the facial cleansers provided in application comparative examples 1 to 7 were used, respectively. The test apparatus is Tewameter TM300 from CK corporation, Germany, and the external environment is tested: room temperature 25 ℃ and humidity 60%. Before using the corresponding facial cleanser and after continuously using the corresponding facial cleanser for 4 days, the change rate of the percutaneous water loss of each subject is respectively tested, each data is tested for three times, after an average value is obtained, the average value of each group is calculated, and one decimal point is reserved.

(3) Moisture content of skin

100 male persons aged 25 to 50 years who had oily skin were selected and randomly divided into 20 groups of 5 persons, and the facial cleansers provided in application examples 1 to 13 and the facial cleansers provided in application comparative examples 1 to 7 were used, respectively. The instrument is of the German Courage + Khazaka electronic type: derma Unit SSC, test temperature 25 ℃, humidity 60%. The skin moisture content of each subject was tested before and 4h after use, three times for each data, and after taking the average, the average of each group was calculated, and one decimal place was retained.

The specific test results are shown in table 6.

TABLE 6

The test data in table 6 show that the facial cleanser prepared by adding the mite-killing conditioner of the invention has the moisture retention rate of more than 80%, the change rate of percutaneous water loss is small and is maintained below 30%, and the moisture content of skin after 4 hours of use is more than 45%. The mite-killing conditioner has the advantages that the compatibility of all components is reasonable, the synergistic effect is realized, the excessive keratinization of the skin can be avoided, the skin moisture retention is improved, and the skin can be kept moist after the composition is used.

Test example 5

Anti-inflammatory Performance test

The main materials are as follows: mouse macrophage line RAW264.7, facial cleanser provided in application examples 1-13 and facial cleanser provided in application examples 1-7, mouse prostaglandin E2(PGE2) ELISA test kit, mouse tumor necrosis factor-alpha (TNF-alpha) ELISA test kit, Lipopolysaccharide (LPS).

(1) For the influence of Lipopolysaccharide (LPS) on the release of PGE2 from RAW264.7 cells, cell supernatant is collected after LPS stimulation for 18h in a control group (serum-free culture medium), PGE2 detection is carried out according to the specification requirements of an ELISA kit, and the inhibition rate is calculated.

(3) And (3) influencing TNF-a release of LPS-stimulated RAW264.7 cells, collecting cell supernatant after LPS stimulation for 18 hours in a control group (serum-free culture medium), carrying out TNF-a detection according to the operation requirements of an ELISA kit specification, and calculating the inhibition rate.

The specific test results are shown in table 7:

TABLE 7

The test data in table 7 show that the facial cleanser prepared by adding the mite-killing conditioner disclosed by the invention has an inhibition rate of more than 80% on PGE2 release and an inhibition rate of more than 80% on TNF-a release, which indicates that the mite-killing conditioner disclosed by the invention has reasonable compatibility of components, has a synergistic effect, has an anti-inflammatory effect, can enhance the skin barrier function, effectively inhibits the release and transfer of inflammatory factors, and has the effects of resisting inflammation and soothing the allergy, so that the inflammatory skin is effectively repaired.

Test example 6

Test of bacteriostatic Property

(1) Preparation of a sample to be tested: selecting filter paper with strong water absorption and uniform texture, making into round paper sheet with diameter of 6mm with a punching machine, drying and sterilizing at 120 deg.C for 2 hr. Adding ten times diluted facial cleanser provided in application examples 1-13 and facial cleanser provided in application examples 1-7 into each paper sheet, uniformly soaking the paper sheets, placing the paper sheets in a sterile plate, drying in an oven at 37 ℃, subpackaging in small bottles, sealing, and storing at 4 ℃ for later use;

(2) respectively dipping freshly prepared diluted bacterial liquids (staphylococcus aureus and propionibacterium acnes) with a sterile cotton stick, extruding redundant bacterial liquids on the tube wall, uniformly coating the bacterial liquids on an NA plate culture medium, circularly sweeping the edge of a plate for one circle, covering, and drying for 2-3 min. The above dried medicated filter paper sheet is applied to the center of the plate with tweezers. Each model strain was made into three groups of parallels, and the average value of the inhibition zone was taken. The above operations are carried out in a biological safety cabinet; wherein, the staphylococcus aureus is cultured for 18h at the constant temperature of 37 ℃, the propionibacterium acnes is cultured for 72h at the constant temperature of 37 ℃ in an anaerobic way, and the size of each inhibition zone is observed and measured;

specific test results are shown in table 8.

TABLE 8

The test data in table 8 show that the facial cleanser prepared by adding the mite removal conditioner disclosed by the invention has obvious effects of inhibiting growth and killing propionibacterium acnes and staphylococcus aureus, wherein the diameter of a bacteriostasis zone of the staphylococcus aureus is 19-24mm, and the diameter of the bacteriostasis zone of the propionibacterium acnes is 16-22mm, so that the mite removal conditioner disclosed by the invention is reasonable in compatibility of components, has a synergistic interaction effect, inhibits growth and reproduction of pathogenic bacteria, and accordingly realizes restoration of skin micro-ecology.

The applicant states that the present invention is illustrated by the above examples to provide the mite-killing conditioner, the facial cleanser and the method for preparing the same, but the present invention is not limited to the above detailed methods, i.e. it is not meant that the present invention must be implemented by relying on the above detailed methods. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.

Claims

1. A mite-killing conditioner, comprising: a mite-killing plant composition, oat beta-glucan, a hydrolyzed yeast extract, a snow lotus herb extract, an aloe extract and tea tree essential oil;

2. The acarus killing conditioner of claim 1, wherein the acarus killing conditioner comprises, by weight: 3-10 parts of mite-removing plant composition, 2-8 parts of oat beta-glucan, 2-8 parts of hydrolytic yeast extract, 1-5 parts of snow lotus herb extract, 1-5 parts of aloe extract and 1-5 parts of tea tree essential oil;

3. The facial cleanser is characterized by comprising the following preparation raw materials: the acarus killing conditioner, surfactant, thickener, pH adjuster, and water of claim 1 or 2.

4. The facial cleanser according to claim 3, wherein the preparation raw materials of the facial cleanser comprise the following components in percentage by mass: the mite-killing conditioner of claim 1 or 2, wherein the mite-killing conditioner comprises 0.1-2% of a surfactant, 20-50% of a thickening agent, 0.5-2% of a pH regulator and the balance of water.

5. The face wash of claim 3 or 4, characterized in that the surfactant comprises any one or a combination of at least two of stearic acid, myristic acid, palmitic acid, lauric acid, monoglyceride, Tween 60, sodium fatty alcohol polyoxyethylene ether sulphate or coconut oil fatty acid diethanolamide, preferably a combination of stearic acid, myristic acid, palmitic acid, lauric acid, monoglyceride, Tween 60, sodium fatty alcohol polyoxyethylene ether sulphate and coconut oil fatty acid diethanolamide;

preferably, the thickener comprises polyethylene glycol distearate and/or sodium hydroxyethyl cellulose, preferably a combination of polyethylene glycol distearate and sodium hydroxyethyl cellulose;

preferably, the pH adjuster is potassium hydroxide.

6. The facial cleanser according to any one of claims 3 to 5, wherein the raw materials for preparing the facial cleanser further comprise any one or a combination of at least two of a humectant, a preservative or an aromatic;

preferably, the raw materials for preparing the facial cleanser also comprise 5-8% of a humectant;

preferably, the humectant is glycerin;

preferably, the raw materials for preparing the facial cleanser also comprise 0.1-1% of preservative;

preferably, the preservative is any one or a combination of at least two of methyl hydroxybenzoate, propyl hydroxybenzoate or phenoxyethanol;

preferably, the raw materials for preparing the facial cleanser also comprise 0.01-0.3% of an aromatic;

preferably, the fragrance is a perfume.

7. The method for preparing the facial cleanser according to any one of claims 3 to 6, comprising the steps of:

8. The method for preparing the facial cleanser according to claim 7, wherein the mixing temperature in the step (1) is 80-85 ℃, and the mixing time is 10-20 min;

preferably, the mixing temperature of the step (2) is 80-85 ℃, and the mixing time is 60-90 min.

9. The method for preparing the facial cleanser according to claim 7 or 8, wherein the mixing temperature in the step (3) is 40 to 50 ℃, and the mixing time is 5 to 15 min;

preferably, the filtration in the step (3) is performed by using 100-300 mesh filter cloth.

10. The method for preparing the facial cleanser according to any one of claims 7 to 9, comprising the steps of: