CN111920732B

CN111920732B - Mite-killing conditioner, shampoo and preparation method thereof

Info

Publication number: CN111920732B
Application number: CN202010813989.3A
Authority: CN
Inventors: 龚德明; 徐洁琼; 胡桂林; 程文曲; 姚文玉
Original assignee: Guangzhou Jinan Cosmetics Co ltd
Current assignee: Guangzhou Jinan Cosmetics Co ltd
Priority date: 2020-08-13
Filing date: 2020-08-13
Publication date: 2023-01-06
Anticipated expiration: 2040-08-13
Also published as: CN111920732A

Abstract

The invention provides a mite-killing conditioner, shampoo and a preparation method thereof. The mite-killing conditioner comprises: mite-killing plant composition, hydrolyzed rice protein, hydrolyzed collagen, fructus Cnidii extract, fructus Sapindi Mukouossi extract and folium Menthae extract; the mite-killing plant composition comprises: rosewood seed extract, zanthoxylum bungeanum fruit extract, sophora flavescens root extract, stemona japonica root extract and phellodendron amurense bark extract. The mite-killing conditioner can also inhibit oil secretion radically by reducing the activity of sebaceous glands on the basis of high-efficiency mite killing. Meanwhile, the mite removing plant composition can promote the exertion of other functional components in the conditioner, thereby achieving the effects of removing dandruff, relieving itching and repairing scalp.

Description

Mite-killing conditioner, shampoo and preparation method thereof

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to a mite-killing conditioner, shampoo and a preparation method thereof.

Background

Recent researches show that mites are closely related to the health of human bodies, particularly mites parasitizing the head and face of the human bodies; it is generally divided into two groups, called follicular mites and sebaceous gland mites, which parasitize the hair follicles and sebaceous glands of human skin, respectively. Generally, the facial part is infected with mites more frequently, but the skin of other parts of the body is infected with mites. If a patient has a severe facial mite infestation, the scalp area will also typically have a mite infestation. Meanwhile, the existing skin care varieties for removing mites are more, but basically have four problems: firstly, the mite removing effect is not obvious, the speed is low, old mites are not removed completely, new mites grow out again, the circulation is repeated, and the mite removing is remote and has no period; secondly, the ingredients have complex sources and low safety, and are easy to cause subacute percutaneous toxicity when being applied to remove mites on the skin; thirdly, the growing environment of the mites is not known, and only the mites are removed, but the mites are not inhibited, so that the problem of the mites is repeated; fourth, in terms of product formulation, the design is rarely performed according to the skin characteristics of men, so that the using effect of most men is poor.

CN104825357A discloses a compound plant component shampoo capable of sterilizing and removing mites, which consists of the following components: deionized water, sodium fatty alcohol sulfate, alkylolamide, coconut fatty acid monoethanolamide, ethylene glycol distearate, cocamidopropyl betaine, guar gum hydroxypropyl trimethyl ammonium oxide, zinc pyrithione, cationic cellulose, citric acid, hyaluronic acid and compound plant mite-inhibiting essential oil. The mite-removing and itching-relieving effects of the shampoo are still to be improved, and the repairing effect on damaged skin is lacked.

CN108245445A discloses a plant formula shampoo for preventing and treating alopecia, repairing scalp, nourishing and growing hair, and resisting bacteria and removing mites, which is prepared from the following raw materials in parts by weight: 75-170 parts of surfactant, 200-250 parts of tea tree essential oil, 1-3 parts of essence and 50 parts of amino acid raw material mixture; the shampoo has improved effects in removing mite, relieving itching, and repairing damaged skin.

Therefore, the development of a men's skin care product which can effectively remove mites and has the functions of controlling oil, relieving itching and repairing damaged scalp is the focus of current research.

Disclosure of Invention

The invention aims to provide a mite-killing conditioner, shampoo and a preparation method thereof. The mite-killing conditioner can also inhibit oil secretion radically by reducing the activity of sebaceous glands on the basis of high-efficiency mite killing. Meanwhile, the mite removing plant composition can promote the exertion of other functional components of the conditioner, thereby achieving the effects of removing dandruff, relieving itching, repairing and maintaining scalp.

In order to achieve the purpose, the invention adopts the following technical scheme:

in a first aspect, the present invention provides a mite control conditioner comprising: mite-killing plant composition, hydrolyzed rice protein, hydrolyzed collagen, fructus Cnidii extract, fructus Sapindi Mukouossi extract and folium Menthae extract;

the mite-killing plant composition comprises: rosewood seed extract, zanthoxylum bungeanum fruit extract, sophora flavescens root extract, stemona japonica root extract and phellodendron amurense bark extract.

In the invention, the active ingredients in the mite removing plant composition are mutually matched, so that the synergistic effect is realized, and the mite removing plant composition can interfere the permeability of the cell membrane of the mites and block the absorption of the cell membrane to nutrient substances, thereby achieving the mite inhibiting effect; and the possibility of inhibiting the reproduction of mites from a food chain is achieved by reducing the activity of pores, inhibiting the secretion of sebum, reducing the activity of sebaceous glands and radically inhibiting the secretion of grease.

In the invention, the active ingredients of the mite removing plant composition, the hydrolyzed rice protein, the hydrolyzed collagen, the cnidium fruit extract, the soapberry extract and the mint leaf extract are matched with each other, so that the synergistic effect is achieved, the mite inhibiting and oil controlling effects of the mite removing plant composition can be further improved, the mite removing plant composition can also promote the exertion of other functional ingredients, and therefore the effects of removing dandruff, relieving itching, repairing and maintaining scalp are achieved, the propagation of fungi in the horny layer of the scalp is inhibited, excessive oil of the scalp is removed, the scalp is nourished while the oil is adsorbed, the water-oil balance of the scalp is maintained, the immunity and the health degree of the scalp are enhanced, the skin of the scalp is nourished, and the damaged part of the scalp is mildly cleaned and repaired.

The hydrolyzed rice protein is protein extracted from hydrolyzed rice, is rich in amino acids and other substances, can promote fibroblasts to generate collagen, can exert high-efficiency moisturizing effect, simultaneously strengthen the defense function of skin, keep normal keratinization of keratinocytes and inhibit bacterial reproduction; the hydrolyzed collagen can well permeate into the bottom layer of the scalp and has good affinity with surrounding tissues, so that the hydrolyzed collagen can assist cells to prepare hydrolyzed collagen, promote the normal growth of the cells in the skin and repair the damaged scalp.

The cnidium monnieri fruit extract (the dandruff removing and itching relieving agent NB-522) is rich in helicid, various phenols and isoflavone, can inhibit the biosynthesis of ergosterol of fungal cell membranes, influences the permeability of the cell membranes, and inhibits the production of the ergosterol to fundamentally block external channels generated by dandruff, so that the dandruff is effectively and radically treated, and the dandruff is not temporarily eliminated from the appearance by means of degreasing and the like. The soapberry extract is an extract of wild soapberry pericarp She Qiaomu, and has main surface active components of triterpenoid saponins (I), sesquiterpene glycosides (II), fatty oil and protein, and has antibacterial, antiinflammatory, dandruff relieving, antipruritic, detergency and foamability. The mint leaf extract has an inhibiting effect on various fungi and escherichia coli which cause skin diseases, and has excellent bacteriostatic, anti-inflammatory and relieving effects.

Preferably, the mite removing conditioner comprises the following components in parts by weight: 5-10 parts of mite-removing plant composition, 2-8 parts of hydrolyzed rice protein, 2-8 parts of hydrolyzed collagen, 1-5 parts of cnidium fruit extract, 1-5 parts of soapberry extract and 1-5 parts of mint leaf extract.

In the mite-killing conditioner, the content of the mite-killing plant composition is 5-10 parts, for example, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts, 10 parts and the like.

In the mite-killing conditioner, the content of the hydrolyzed rice protein is 2 to 8 parts, and may be, for example, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, and the like.

In the mite-killing conditioner, the content of the hydrolyzed collagen is 2 to 8 parts, and may be, for example, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, or the like.

In the mite-killing conditioner, the content of the cnidium fruit extract is 1-5 parts, such as 1 part, 2 parts, 3 parts, 4 parts, 5 parts and the like.

In the mite-killing conditioner, the content of the soapberry extract is 1-5 parts, such as 1 part, 2 parts, 3 parts, 4 parts, 5 parts and the like.

In the mite-killing conditioner, the content of the mint leaf extract is 1-5 parts, such as 1 part, 2 parts, 3 parts, 4 parts, 5 parts and the like.

Preferably, the mite removing plant composition comprises the following components in parts by weight: 5-10 parts of rosewood seed extract, 5-10 parts of zanthoxylum bungeanum fruit extract, 5-10 parts of sophora flavescens root extract, 1-5 parts of radix stemonae petiolata root extract and 1-5 parts of phellodendron bark extract.

In the mite removing plant composition, the content of the rosewood seed extract is 5-10 parts, such as 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts and the like.

In the mite removing plant composition, the content of the zanthoxylum fruit extract is 5-10 parts, for example, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts and the like.

In the mite-killing plant composition, the content of the sophora flavescens root extract is 5-10 parts, such as 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts and the like.

In the mite removing plant composition, the content of the stemona tuberosa root extract is 1-5 parts, and can be 1 part, 2 parts, 3 parts, 4 parts, 5 parts and the like, for example.

In the mite-removing plant composition, the content of the phellodendron amurense bark extract is 1-5 parts, such as 1 part, 2 parts, 3 parts, 4 parts, 5 parts and the like.

In a second aspect, the invention provides a shampoo, and the preparation raw materials of the shampoo comprise: a mite control conditioner as described in the first aspect, a surfactant, a thickener, a chelating agent, and water.

Preferably, the preparation raw materials of the shampoo comprise the following components in percentage by mass: 0.1-2% of mite-killing conditioner, 20-40% of surfactant, 0.1-0.5% of thickening agent, 0.05-0.2% of chelating agent and the balance of water.

The amount of the mite-killing conditioner added in the first aspect is 0.1 to 2% by mass of 100% of the raw materials for preparing the shampoo, and may be, for example, 0.1%, 0.2%, 0.4%, 0.6%, 0.8%, 0.9%, 1%, 1.2%, 1.4%, 1.6%, 1.8%, 2% and the like.

The amount of the surfactant added is 20 to 40% based on 100% by mass of the raw materials for preparing the shampoo, and may be, for example, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 28%, 30%, 32%, 33%, 34%, 36%, 37%, 38%, 40%, etc.

The addition amount of the thickener is 0.1-0.5%, for example, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, etc., based on 100% of the preparation raw material of the shampoo.

The addition amount of the chelating agent is 0.05-0.2%, for example, 0.05%, 0.08%, 0.1%, 0.12%, 0.14%, 0.16%, 0.18%, 0.2%, etc., based on 100% of the preparation raw material of the shampoo.

Preferably, the surfactant comprises any one or a combination of at least two of sodium fatty alcohol polyoxyethylene ether sulfate, ammonium lauryl sulfate, coconut fatty acid monoethanolamide, pearlescene, cocamidopropyl betaine or sodium lauroamphoacetate, preferably a combination of sodium fatty alcohol polyoxyethylene ether sulfate, ammonium lauryl sulfate, coconut fatty acid monoethanolamide, pearlescene, cocamidopropyl betaine and sodium lauroamphoacetate.

The pearly-lustre sheet comprises ethylene glycol distearate and ethylene glycol monostearate, can produce obvious pearly-lustre effect, can increase the viscosity of the product, and also has the functions of moistening skin, nourishing and protecting hair and resisting static electricity. The pearl essence has good intermiscibility with other types of surfactants, can embody the stable pearl effect and the thickening and conditioning functions, has no stimulation to skin and no damage to hair.

As a preferable technical scheme, the surfactant is a combination of fatty alcohol-polyoxyethylene ether sodium sulfate, lauryl sodium sulfate ammonium, coconut oil fatty acid monoethanolamide, a pearly sheet, cocamidopropyl betaine and lauroamphoacetate, all the components are matched with each other, so that a synergistic effect is achieved, rich and dense foam can be quickly formed after the surfactant is wetted with water, grease and dirt on the skin can be taken away, the dispersibility and the permeability of the mite-removing conditioner can be improved, the absorption utilization rate of scalp skin on all active components in shampoo can be improved, and the synergistic mite-removing conditioner can achieve better effects of controlling oil, removing oil, nourishing and repairing scalp.

Preferably, the thickener is a carbomer.

Preferably, the chelating agent is EDTA-2Na.

Preferably, the preparation raw materials of the shampoo further comprise any one or a combination of at least two of a hair conditioner, a pH regulator, a preservative or a fragrance.

Preferably, the preparation raw material of the shampoo further comprises 0.1-1% of a hair conditioner based on 100% of the preparation raw material of the shampoo, and the hair conditioner can be 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1% and the like.

Preferably, the hair conditioning agent comprises cationic guar and/or cationic cellulose, preferably a combination of cationic guar and cationic cellulose.

The cationic guar gum can show excellent antistatic property and conditioning effect after being adsorbed on hair, improves the wet combing property of the hair, and enables the hair to keep luster, softness and high elasticity for a long time; the cationic cellulose is JR-400 cationic cellulose. The JR-400 has excellent conditioning property, can repair damaged hair, and improves the smoothness, smoothness and antistatic property of the hair. The two components are matched with each other, have synergistic effect, and can further improve the dandruff removing and itching relieving capabilities of the shampoo.

Preferably, the raw materials for preparing the shampoo further comprise 0.1-1% of a pH regulator, such as 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, etc.

Preferably, the pH adjusting agent is TEA.

Preferably, the preparation raw material of the shampoo further comprises 0.1-0.5% of preservative, for example, 0.1%, 0.2%, 0.3%, 0.4%, 0.5% and the like, based on 100% of the preparation raw material of the shampoo.

Preferably, the preservative is DMDM hydantoin.

Preferably, the preparation raw material of the shampoo further comprises 0.01-0.3% of aromatic, for example, 0.01%, 0.02%, 0.04%, 0.06%, 0.08%, 0.1%, 0.15%, 0.2%, 0.25%, 0.3% and the like, based on 100% of the preparation raw material of the shampoo.

Preferably, the fragrance is a perfume.

In a third aspect, the invention provides a preparation method of the shampoo according to the second aspect, and the preparation method of the shampoo comprises the following steps:

(1) Mixing a thickening agent, a chelating agent and water to obtain a first mixed solution;

(2) Mixing the first mixed solution obtained in the step (1) with a surfactant to obtain a second mixed solution;

(3) And (3) mixing the second mixed solution obtained in the step (2) with the mite removing conditioner, and filtering to obtain the shampoo.

Preferably, the mixing temperature in step (1) is 80-85 deg.C, such as 80 deg.C, 81 deg.C, 82 deg.C, 83 deg.C, 84 deg.C, 85 deg.C, etc., and the mixing time is 5-10min, such as 5min, 6min, 7min, 8min, 9min, 10min.

Preferably, the mixing temperature in step (2) is 60-70 deg.C, such as 60 deg.C, 62 deg.C, 64 deg.C, 66 deg.C, 68 deg.C, 70 deg.C, etc., and the mixing time is 5-15min, such as 5min, 7min, 9min, 11min, 13min, 15min, etc.

Preferably, the mixing temperature in step (3) is 40-50 deg.C, such as 40 deg.C, 42 deg.C, 44 deg.C, 46 deg.C, 48 deg.C, 50 deg.C, etc., and the mixing time is 5-15min, such as 5min, 7min, 9min, 11min, 13min, 15min, etc.

Preferably, the filtration in step (3) is performed by using 100-300 mesh filter cloth, for example, 100 mesh, 120 mesh, 140 mesh, 160 mesh, 180 mesh, 200 mesh, 220 mesh, 240 mesh, 260 mesh, 280 mesh, 300 mesh, etc.

Preferably, the preparation method of the shampoo comprises the following steps:

(1) Mixing a thickening agent, a chelating agent and water at 80-85 ℃ for 5-10min to obtain a first mixed solution;

(2) Mixing the first mixed solution obtained in the step (1) with a surfactant at 60-70 ℃ for 5-15min to obtain a second mixed solution;

(3) And (3) mixing the second mixed solution obtained in the step (2), the mite removing conditioner, the hair conditioner, the pH regulator, the preservative and the aromatic at 40-50 ℃ for 5-15min, and filtering by using filter cloth of 100-300 meshes to obtain the shampoo.

Compared with the prior art, the invention has the following beneficial effects:

(1) The mite-killing conditioner can kill mites efficiently, inhibit grease secretion radically by reducing the activity of sebaceous glands, and inhibit the absorption of cell membranes to nutrient substances by interfering the permeability of the cell membranes of the mites, so that the mite-killing effect is achieved; meanwhile, the mite removing plant composition can promote the exertion of other functional components in the conditioner, thereby achieving the effects of removing dandruff, relieving itching, repairing and maintaining scalp;

(2) In a mite removing test of shampoo prepared by adding the mite removing conditioner, the insecticidal rate is more than 96%, the reduction rate of the content of scalp grease is more than 40%, and the inhibition rate of malassezia is more than 85%.

Detailed Description

The technical solution of the present invention is further explained by the following embodiments. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention.

Preparation example 1

The preparation example provides a rosewood seed extract, which is prepared by the following preparation method:

(1) Crushing rosewood seeds to a particle size of 50 meshes, mixing the rosewood seeds with 70wt% of ethanol water solution according to a mass ratio of 1;

(2) Centrifuging the rosewood seed extracting solution obtained in the step (1) at the rotating speed of 7000r/min by adopting a disc centrifuge, and concentrating by adopting a reverse osmosis membrane, wherein the concentration pressure is 10bar to obtain a crude rosewood seed extract;

(3) Mixing the crude extract of the rosewood seeds obtained in the step (2) with maltodextrin in a mass ratio of 4:6 for ultrasonic treatment, wherein the ultrasonic treatment time is 60min, the ultrasonic treatment power is 300W, and freezing at-15 ℃ to obtain the rosewood seed extract.

Preparation example 2

The preparation example provides a zanthoxylum bungeanum maxim fruit extract, which is prepared by the following preparation method: crushing dry pricklyash peel to 150 meshes, mixing the crushed dry pricklyash peel with petroleum ether, standing and extracting, mixing the mixture with acetone, wherein the mass ratio of the dry pricklyash peel to the petroleum ether is 1:2, standing and extracting, and then mixing the mixture with the acetone, and the mass ratio of the dry pricklyash peel to the acetone is 1:3, standing and extracting, and filtering to obtain filtrate; mixing and stirring the obtained filtrate with a 2wt% sodium bicarbonate solution, mixing the dried pricklyash peel fruits and the 2wt% sodium bicarbonate solution with a mass ratio of 1.

Preparation example 3

The preparation example provides a kuh-seng root extract, which is prepared by the following preparation method: crushing the sophora flavescens ait roots to 150 meshes, mixing the sophora flavescens ait roots with 75wt% of ethanol water solution according to the mass ratio of 1.

Preparation example 4

The preparation example provides a stemona tuberosa root extract, which is prepared by the following preparation method:

(a) Crushing the stemona tuberosa roots to a particle size of 150 meshes, mixing the stemona tuberosa roots with water according to a mass ratio of 1;

(b) Microfiltration is carried out on the water extraction product obtained in the step (a), the microfiltration is carried out by adopting a ceramic membrane, the number of pores of the ceramic membrane is 35, the separation precision of the ceramic membrane is 80nm, then ultrafiltration is carried out, and the molecular weight of the interception pore diameter of ultrafiltration membrane separation equipment is larger than 1000, so that filter residue and filtrate are obtained;

(c) Mixing the filter residue obtained in the step (b) with ethanol for alcohol precipitation to obtain an extract I, concentrating the filtrate obtained in the step (b), performing spray drying at 40 ℃ and under 30MPa to obtain an extract II, and combining the extract I and the extract II to obtain the stemona tuberosa root extract.

Preparation example 5

The preparation example provides a phellodendron amurense bark extract, which is prepared by the following preparation method:

(A) Pulverizing cortex Phellodendri bark to 150 mesh, mixing with n-hexane at a mass ratio of 1:2, soaking at 30 deg.C for 3h, filtering, and drying to obtain defatted cortex Phellodendri bark powder;

(B) Mixing the degreased phellodendron amurense bark powder obtained in the step (A) with 60wt% ethanol water solution according to the mass ratio of 1;

(C) Dissolving the coarse extract of the phellodendron amurense bark obtained in the step (B) by using 10wt% ethanol water solution, purifying by using a D4020 macroporous resin gel column, eluting by using 60wt% ethanol water solution, and concentrating to obtain the phellodendron amurense bark extract.

Preparation example 6

The preparation example provides a mite removing plant composition I, which is prepared by the following preparation method: mixing and stirring 8 parts of rosewood seed extract, 6 parts of zanthoxylum bungeanum fruit extract, 6 parts of sophora flavescens root extract, 2 parts of She Baibu root extract and 4 parts of phellodendron amurense bark extract at 25 ℃ for 10min to obtain the mite removing plant composition I.

Preparation example 7

The preparation example provides a mite removing plant composition II, which is prepared by the following preparation method: mixing and stirring 6 parts of red wood seed extract, 5 parts of pricklyash peel extract, 9 parts of radix sophorae flavescentis extract, 4 parts of She Baibu root extract and 2 parts of phellodendron amurense bark extract at 25 ℃ for 10min to obtain the mite removing plant composition II.

Preparation example 8

The preparation example provides a mite removing plant composition III, which is prepared by the following preparation method: mixing 7 parts of semen Oroxyli extract, 8 parts of fructus Zanthoxyli extract, 5 parts of radix Sophorae Flavescentis extract, 3 parts of She Baibu root extract and 3 parts of cortex Phellodendri bark extract at 25 deg.C, stirring for 10min to obtain mite-killing plant composition III.

The mite-removing plant composition in the following examples and comparative examples was prepared by the above-mentioned preparation method, and other components were commercially available.

Example 1

The embodiment provides a mite removing conditioner, which comprises the following components in parts by weight: the mite-killing plant composition I provided by the preparation example 6 comprises 8 parts of hydrolyzed rice protein, 5 parts of hydrolyzed collagen, 2 parts of cnidium fruit extract, 3 parts of soapberry extract and 2 parts of mint leaf extract.

Example 2

The embodiment provides a mite removing conditioner, which comprises the following components in parts by weight: preparation example 7 provides 7 parts of mite-killing plant composition II, 6 parts of hydrolyzed rice protein, 4 parts of hydrolyzed collagen, 3 parts of fructus cnidii extract, 4 parts of soapberry extract and 1 part of mint leaf extract.

Example 3

The embodiment provides a mite removing conditioner, which comprises the following components in parts by weight: 6 parts of mite-killing plant composition III, 4 parts of hydrolyzed rice protein, 6 parts of hydrolyzed collagen, 4 parts of cnidium fruit extract, 2 parts of soapberry extract and 3 parts of mint leaf extract, which are provided in preparation example 8.

Example 4

The embodiment provides a mite removing conditioner, which comprises the following components in parts by weight: the mite-killing plant composition I provided by the preparation example 6 comprises 4 parts of hydrolyzed rice protein, 4 parts of hydrolyzed collagen, 5 parts of fructus cnidii extract, 4 parts of soapberry extract and 4 parts of mint leaf extract.

Example 5

The embodiment provides a mite removing conditioner, which comprises the following components in parts by weight: the mite-killing plant composition I provided by the preparation example 6 comprises 12 parts of mite-killing plant composition I, 4 parts of hydrolyzed rice protein, 3 parts of hydrolyzed collagen, 2 parts of fructus cnidii extract, 2 parts of soapberry extract and 2 parts of mint leaf extract.

Comparative examples 1 to 6

Comparative examples 1-6 each provide different mite control conditioners having the formula in parts by weight shown in table 1 below:

TABLE 1

Application example 1

The application embodiment provides shampoo, and the preparation raw materials of the shampoo comprise the following components in percentage by mass:

the preparation method of the shampoo comprises the following steps:

(1) Mixing a thickening agent, a chelating agent and water at 82 ℃ for 8min to obtain a first mixed solution;

(2) Mixing the first mixed solution obtained in the step (1) with a surfactant at 65 ℃ for 10min to obtain a second mixed solution;

(3) And (3) mixing the second mixed solution obtained in the step (2), the mite removing conditioner, the hair conditioner, the pH regulator, the preservative and the aromatic at 45 ℃ for 10min, and filtering by using filter cloth of 200 meshes to obtain the shampoo.

Application example 2

The shampoo provided by the application example is different from the shampoo provided by the application example 1 in that the mite removing conditioner provided by the example 1 is replaced by the mite removing conditioner provided by the example 2, and the content of other components and the preparation method are the same as those of the shampoo provided by the application example 1.

Application example 3

The shampoo provided by the application example is different from the shampoo provided by the application example 1 in that the mite removing conditioner provided by the example 1 is replaced by the mite removing conditioner provided by the example 3, and the content of other components and the preparation method are the same as those of the shampoo provided by the application example 1.

Application example 4

The shampoo provided by the application example is different from the shampoo provided by the application example 1 in that the mite removing conditioner provided by the example 1 is replaced by the mite removing conditioner provided by the example 4, and the content of other components and the preparation method are the same as those of the shampoo provided by the application example 1.

Application example 5

The shampoo provided by the application example is different from the shampoo provided by the application example 1 in that the mite removing conditioner provided by the example 1 is replaced by the mite removing conditioner provided by the example 5, and the content of other components and the preparation method are the same as those of the shampoo provided by the application example 1.

Application example 6

The application example provides shampoo, and the shampoo is different from the application example 1 in that the surfactant does not contain fatty alcohol polyoxyethylene ether sodium sulfate, the content of cocamidopropyl betaine is increased to 6%, the content of coconut fatty acid monoethanolamide is increased to 3.5%, the content of pearlescent flakes is increased to 4%, the content of cocamidopropyl betaine is increased to 11%, the content of sodium lauroamphoacetate is increased to 6%, and the content and the preparation method of other components are the same as the application example 1.

Application example 7

The application example provides shampoo, and the shampoo is different from the shampoo in the application example 1 in that the surfactant does not contain cocamidopropyl betaine, the content of sodium fatty alcohol polyoxyethylene ether sulfate is increased to 15.6%, the content of coconut fatty acid monoethanolamide is increased to 1.1%, the content of a pearlescent piece is increased to 1.6%, the content of cocamidopropyl betaine is increased to 8.6%, the content of sodium lauroamphoacetate is increased to 3.6%, and the content and the preparation method of other components are the same as those in the application example 1.

Application example 8

The application example provides a shampoo, and is different from the application example 1 in that the surfactant does not contain coconut fatty acid monoethanolamide, the content of the fatty alcohol polyoxyethylene ether sodium sulfate is increased to 15.1%, the content of the cocamidopropyl betaine is increased to 3.1%, the content of a pearlescent piece is increased to 1.1%, the content of the cocamidopropyl betaine is increased to 8.1%, the content of sodium lauroamphoacetate is increased to 3.1%, and the content and the preparation method of other components are the same as those in the application example 1.

Application example 9

The application example provides shampoo, and the shampoo is different from the shampoo in the application example 1 in that the surfactant does not contain a pearly-lustre sheet, the content of the sodium fatty alcohol polyoxyethylene ether sulfate is increased to 15.2%, the content of the cocamidopropyl betaine is increased to 3.2%, the content of the coconut fatty acid monoethanolamide is increased to 0.7%, the content of the cocamidopropyl betaine is increased to 8.2%, the content of the sodium lauroamphoacetate is increased to 3.2%, and the content and the preparation method of other components are the same as those in the application example 1.

Application example 10

The application example provides shampoo, and the shampoo is different from the shampoo in the application example 1 in that the surfactant does not contain cocamidopropyl betaine, the content of sodium fatty alcohol polyoxyethylene ether sulfate is increased to 16.6%, the content of cocamidopropyl betaine is increased to 4.6%, the content of coconut fatty acid monoethanolamide is increased to 2.1%, the content of pearlescent flakes is increased to 2.6%, the content of sodium lauroamphoacetate is increased to 4.6%, and the content of other components and the preparation method are the same as those in the application example 1.

Application example 11

The application example provides shampoo, and the shampoo is different from the shampoo in the application example 1 in that the surfactant does not contain sodium lauroamphoacetate, the content of the sodium fatty alcohol polyoxyethylene ether sulfate is increased to 15.6%, the content of the cocamidopropyl betaine is increased to 3.6%, the content of the coconut fatty acid monoethanolamide is increased to 1.1%, the content of the pearlescent piece is increased to 1.6%, the content of the cocamidopropyl betaine is increased to 8.6%, and the content of other components and the preparation method are the same as those in the application example 1.

Application example 12

The application example provides shampoo, and the shampoo is different from the application example 1 in that the hair conditioner does not contain cationic guar gum, the content of cationic cellulose is increased to 0.3%, and the content of other components and the preparation method are the same as the application example 1.

Application example 13

The shampoo provided by the application example is different from the shampoo provided by the application example 1 in that the hair conditioner does not contain cationic cellulose, the content of the cationic guar gum is increased to 0.3%, and the content of other components and the preparation method are the same as those of the application example 1.

Application of comparative examples 1 to 6

Compared with the application example 1, the mite removing conditioner provided by the example 1 is respectively and correspondingly replaced by the mite removing conditioner provided by the comparative examples 1-6, and the content of other components and the preparation method are the same as the application example 1.

Application comparative example 7

Compared with the shampoo prepared in the application example 1, the shampoo is not added with a mite removing conditioner, the shortage part is supplemented to 100% by water, and the contents of other components and the preparation method are the same as those of the shampoo prepared in the application example 1.

Test example 1

Safety performance testing

Safety tests were carried out on the shampoos provided in application examples 1 to 13 and on the shampoos provided in application comparative examples 1 to 7, by the following methods:

(1) Haemolysis test of erythrocytes

Preparation of erythrocyte suspension: selecting healthy rabbit, taking 9mL of blood from heart, adding 1mL of 2% potassium oxalate solution, centrifuging, discarding supernatant, diluting the precipitate to 20mL with 20mmol/L PBS solution, and storing at 4 ℃ for later use. Select samples were diluted with PBS solution to different concentrations, with 5 concentration gradients set for each sample. Taking 10mL of a dilution of a sample to be detected, adding 200 μ L of the erythrocyte suspension (controlling the final concentration of the sample to be 5, 10, 20, 50 and 100mg/mL respectively), using distilled water as a full-blood-soluble control and PBS solution as a negative control, gently mixing, incubating at 37 ℃ for 30min, centrifuging at 2000r/min for 10min, taking the supernatant, and testing the absorbance (A) at 560nm by using a spectrophotometer ₅₆₀ ) Calculating the hemolysis rate according to the following formula;

a standard curve of hemolysis rate vs. sample concentration was plotted, and the sample concentration at which hemolysis occurred in 50% erythrocytes (HD) was calculated ₅₀ )。

(2) Protein denaturation experiments:

diluting the sample to 10g/L with PBS solution, collecting 10mL of the dilution of the sample to be tested, adding 200 μ L of the erythrocyte suspension, using distilled water as blank control, 1mg/mL Sodium Dodecyl Sulfate (SDS) solution as positive control, mixing gently, incubating at 37 deg.C for 30min, centrifuging at 2000r/min for 10min, collecting the supernatantThe clear solution was measured for absorbance A at 540nm and 575nm with a spectrophotometer ₅₄₀ And A ₅₇₅ Calculating a protein Denaturation Index (DI) according to the following formula;

wherein R is ₁ = blank control group a ₅₇₅ Blank control group A ₅₄₀ ，R ₂ = Experimental group A ₅₇₅ Experimental group A ₅₄₀ ，R ₃ = positive control group a ₅₇₅ Positive control group A ₅₄₀ 。

Evaluating the irritation of the sample to be tested according to the L/D value, wherein the L/D value is HD ₅₀ DI, erythrocyte hemolysis assay irritation grading criteria are shown in Table 2 below:

TABLE 2

L/D	Grading
		>100	Has no irritation
10<L/D≤100	Micro-stimulation property
		1<L/D≤10	Mild irritation
0.1<L/D≤1	Moderate irritation

The results of the above-described hemolysis test and protein denaturation test are shown in Table 3 below:

TABLE 3

As can be seen from the safety performance test, the shampoo prepared by the application examples 1-13 is mild and non-irritant according to the test results; the sample concentration HD of 50% erythrocyte of the shampoo prepared by the invention is hemolytic ₅₀ HD higher than 15000mg/L than that of shampoo prepared by using comparative examples 1-7 ₅₀ (ii) a Meanwhile, the protein denaturation index DI is below 5 percent and is obviously smaller than that of the shampoo prepared by the application comparative examples 1-7, which shows that the toxic and side effects and the irritation of the shampoo can be obviously reduced by adding the mite removing conditioner, and the shampoo is safer and more reliable.

Test example 2

Mite killing rate test

(1) Acquisition of human demodex: adopting a transparent adhesive tape sticking method to obtain the demodex, and specifically comprising the following steps: the examinee is ordered to wash face before sleeping, and then apply transparent adhesive tapes with length of 6cm and width of 1.5cm to the forehead, cheek, nose, chin, etc., and then take off the transparent adhesive tapes and stick the transparent adhesive tapes to the adhesive tapes next morning, and then carry out microscopic examination and seal the transparent adhesive tapes on a glass slide.

(2) Grouping tests: each glass slide is dripped with 0.1mg/cm ² The shampoo provided in application examples 1 to 13 and the shampoo provided in application comparative examples 1 to 7 were applied so that the shampoo was spread evenly and sufficient contact between the shampoo and the insect bodies was ensured. Placing in an environment with relative humidity of 70-80% and temperature of 26-28 deg.C, taking out after 24 hr, and observing demodex survival condition under microscope. After continuously observing under a 400X microscope for 3min, the dead insect is judged to be dead by the dead insect.

The specific test results are shown in table 4 below:

TABLE 4

Test sample	Insecticidal rate/%)	Test sample	Insecticidal efficiency/%
				Application example 1	99.9	Application example 11	97.8
Application example 2	99.8	Application example 12	96.8
				Application example 3	99.6	Application example 13	96.9
Application example 4	99.0	Comparative application example 1	70.2
				Application example 5	98.8	Comparative application example 2	90.2
Application example 6	97.5	Comparative application example 3	88.6
				Application example 7	97.6	Comparative application example 4	89.2
Application example 8	98.3	Comparative application example 5	90.5
				Application example 9	98.5	Comparative application example 6	89.9
Application example 10	98.4	Application comparative example 7	38.4

The test results in table 4 show that the insecticidal rate of the shampoo mite-killing prepared by adding the mite-killing conditioner of the invention is more than 96%, which indicates that the mite-killing plant composition, the hydrolyzed rice protein, the hydrolyzed collagen, the fructus cnidii extract, the soapberry extract and the mint leaf extract in the mite-killing conditioner of the invention are mutually matched, so that the synergistic effect is achieved, the sebaceous gland activity is reduced, the grease secretion is inhibited from being rooted, and the absorption of cell membranes to nutrient substances is inhibited by interfering the permeability of the cell membranes of mites while the mite-killing is performed efficiently.

Test example 3

Scalp oil control Effect test

Scalp oil content testing: 100 volunteers, male men aged 25-50 years, were selected and randomly divided into 20 groups of 5 persons, and each group was used to take appropriate amount of shampoo provided in application examples 1-13 and shampoo provided in application comparative examples 1-7 to clean scalp. During the test period, hair bleaching and dyeing were prohibited, other care products could not be used, and subjects could not stop using the products except in special cases. The scalp oil secretion state is evaluated by adopting SEBUMETER to collect the scalp oil content. The SEBUMETER method is based on the principle of photometer, a special extinction adhesive tape with the thickness of 0.1mm absorbs grease on human skin and then becomes a semitransparent adhesive tape, the light transmittance of the adhesive tape changes, the more grease is absorbed, the greater the light transmittance is, and therefore the content of scalp grease can be measured. The test conditions are strictly that the temperature is 22 +/-1 ℃, each sample to be tested is used for 8 hours after hair washing, then 10 different parts of the scalp are measured by SEBUMER, the average value is obtained, and the reduction rate of the content of the scalp grease after each sample is used is calculated;

the specific test results are shown in table 5:

TABLE 5

The test data in table 5 show that the content reduction rate of the shampoo prepared by adding the mite removing conditioner disclosed by the invention on scalp grease is more than 40%, which indicates that the mite removing plant composition, the hydrolyzed rice protein, the hydrolyzed collagen, the fructus cnidii extract, the soapberry extract and the mint leaf extract in the mite removing conditioner disclosed by the invention are matched with each other, so that the synergistic effect is achieved, grease secretion can be inhibited through reducing the activity of sebaceous glands and root sources, and the oil control effect is excellent.

Test example 4

In vitro dandruff removal and itching relieving effect test

(1) Test samples: applying the shampoo provided in examples 1-13 and applying the shampoo provided in comparative examples 1-7;

(2) The test principle is as follows: the malassezia bacteria mainly grow in the stratum corneum, and the growth speed of the malassezia bacteria is increased, so that the microenvironment on the surface of the head skin is destroyed, and the maculopapule and the skin desquamation appear on the surface of the head skin, thereby generating the itching of the head and dandruff, inhibiting the growth of the malassezia bacteria, and inhibiting the generation of the dandruff and the itching of the head;

(3) The test method comprises the following steps: preparing malassezia furfur into bacterial suspension by using normal saline, diluting the bacterial suspension into bacterial suspension with the turbidity of more than 2.0McF, diluting the bacterial suspension by using normal saline by 100 times, diluting the bacterial suspension by using RPMI 1640 by 10 times, equally dividing the obtained bacterial suspension into 21 parts, respectively adding each group of test samples to be tested into the bacterial suspension, transferring each part of bacterial suspension into a 96-hole culture plate, culturing for 72-h, slightly shaking, carrying out color comparison at 490nm, and calculating the inhibition rate of each sample to be tested on the malassezia furfur.

The specific test results are shown in table 6:

TABLE 6

As can be seen from the test data in Table 6, the inhibition rate of the shampoo prepared by adding the mite-killing conditioner disclosed by the invention on malassezia is over 85%. The mite-removing plant composition, the hydrolyzed rice protein, the hydrolyzed collagen, the cnidium fruit extract, the soapberry extract and the mint leaf extract in the mite-removing conditioner are matched with each other, have a synergistic effect, can effectively inhibit the mass proliferation of malassezia, solve the problem that the microenvironment of the surface of the head skin is damaged, and further relieve the phenomena of maculopapule and skin desquamation on the surface of the head skin.

Test example 5

Clinical scalp repair test:

(1) Selecting 100 volunteers with severe itching and swelling scalp and male with age of 25-50 years, randomly dividing into 20 groups, 5 persons in each group, and respectively taking appropriate amount of shampoo provided in application examples 1-13 and shampoo provided in application comparative examples 1-7 to clean scalp in each group; the shampoo using method comprises the following steps: the shampoo is used for washing hair for 4 days of each person, and 10mL of the shampoo is used for washing hair for 40 days. Meanwhile, the experimenter observes the amount of dandruff and the maximum dandruff diameter before shampooing of the test population, records the scores of all groups, and calculates the average value of the scores of all groups;

dandruff evaluation criteria: 5 min-no scurf; 4 min-small powdery grey rough scale; 3 min-small to medium sized chips; 2 min-large, thin chips loosely associated with the scalp; 1 min-large adhesive chips; 0 point-white to yellow thick scales that adhere tightly to the scalp;

(2) Selecting 105 volunteers with severe itching and red swelling of scalp and male with age of 25-50 years, randomly dividing into 21 groups, 5 persons in each group, and respectively taking appropriate amount of shampoo provided in application examples 1-14 and shampoo provided in application comparative examples 1-7 to clean scalp in each group; the shampoo using method comprises the following steps: the shampoo is used for washing hair for 4 days of each person, and 10mL of the shampoo is used for washing hair for 40 days. Meanwhile, the experimenter observes the quantity of red and swollen bags on the scalp and inquires the strong degree of itching feeling before the test population washes hair.

Evaluation standard of scalp pruritus and red swelling: 5 min-no phenomenon; score 4-not evident; 3 min-slight; 2 min-medium; score 1-severity; 0 point-very severe.

The specific test results are shown in table 7:

TABLE 7

As can be seen from the test results in Table 7, the problems of dandruff on the scalp and pruritus and redness on the scalp after 40 days of using the shampoo provided by the invention are obviously relieved. The mite-removing plant composition, the hydrolyzed rice protein, the hydrolyzed collagen, the cnidium monnieri fruit extract, the soapberry fruit extract and the mint leaf extract in the mite-removing conditioner are matched with each other, so that the synergistic effect is achieved, the effects of removing dandruff, relieving itching, repairing and maintaining scalp are achieved, the propagation of fungi in the horny layer of the scalp is inhibited, excessive oil of the scalp is removed, the scalp is nourished while the oil is adsorbed, the water-oil balance of the scalp is kept, the immunity and the health degree of the scalp are enhanced, the skin of the scalp is nourished, and the damaged part of the scalp is gently cleaned and repaired.

The applicant states that the present invention is illustrated by the above examples to provide mite control conditioners, shampoos and methods of making the same, but the present invention is not limited to the details described above, i.e., it is not intended that the invention be construed as necessarily requiring such details to be incorporated herein. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.

Claims

1. The mite removing conditioner is characterized by comprising the following components in parts by weight: 5-10 parts of mite-removing plant composition, 2-8 parts of hydrolyzed rice protein, 2-8 parts of hydrolyzed collagen, 1-5 parts of cnidium fruit extract, 1-5 parts of soapberry extract and 1-5 parts of mint leaf extract;

the mite removing plant composition comprises the following components in parts by weight: 5-10 parts of rosewood seed extract, 5-10 parts of zanthoxylum bungeanum fruit extract, 5-10 parts of sophora flavescens root extract, 1-5 parts of stemona tuberosa root extract and 1-5 parts of phellodendron amurense bark extract.

2. The shampoo is characterized in that the preparation raw materials of the shampoo comprise: the acarus killing conditioner of claim 1, surfactant, thickener, chelating agent, and water.

3. The shampoo as claimed in claim 2, wherein the preparation raw materials of the shampoo comprise, by mass: the mite-killing conditioner of claim 1, wherein the mite-killing conditioner comprises 0.1-2% of a surfactant, 20-40% of a thickener, 0.1-0.5% of a chelating agent, 0.05-0.2% of a chelating agent, and the balance of water.

4. The shampoo according to claim 2, wherein the surfactant comprises any one or a combination of at least two of sodium fatty alcohol polyoxyethylene ether sulfate, ammonium lauryl sulfate, coconut oil fatty acid monoethanolamide, pearlescene, cocamidopropyl betaine, or sodium lauroamphoacetate.

5. The shampoo according to claim 4 wherein the surfactant is a combination of sodium fatty alcohol polyoxyethylene ether sulfate, ammonium lauryl sulfate, coconut monoethanolamide, pearlescene, cocamidopropyl betaine, and sodium lauroamphoacetate.

6. The shampoo according to claim 2, wherein the thickener is a carbomer.

7. The shampoo according to claim 2, wherein the chelating agent is EDTA-2Na.

8. The shampoo according to claim 2, wherein the preparation raw materials of the shampoo further comprise any one or a combination of at least two of a hair conditioner, a pH regulator, a preservative or a fragrance.

9. The shampoo according to claim 8, wherein the preparation raw materials of the shampoo further comprise 0.1-1% of a hair conditioner.

10. The shampoo according to claim 9 wherein the hair conditioner comprises a cationic guar and/or a cationic cellulose.

11. The shampoo according to claim 10, wherein the hair conditioning agent is a combination of a cationic guar gum and a cationic cellulose.

12. The shampoo according to claim 8, wherein the raw materials for preparing the shampoo further comprise 0.1-1% of a pH regulator.

13. The shampoo according to claim 12, wherein the pH modifier is TEA.

14. The shampoo as claimed in claim 8, wherein the raw materials for preparing the shampoo further comprise 0.1-0.5% of a preservative.

15. The shampoo of claim 14 wherein the preservative is DMDM hydantoin.

16. The shampoo according to claim 8, wherein the preparation raw materials of the shampoo further comprise 0.01-0.3% of a fragrance.

17. The shampoo of claim 16, wherein the fragrance is a perfume.

18. Method for preparing a shampoo according to any of claims 2 to 17, characterized in that it comprises the following steps:

(3) And (3) mixing the second mixed solution obtained in the step (2) with the mite-removing conditioner, and filtering to obtain the shampoo.

19. The method for preparing the shampoo according to claim 18, wherein the mixing temperature in the step (1) is 80-85 ℃, and the mixing time is 5-10min.

20. The method for preparing the shampoo according to claim 18, wherein the mixing temperature in the step (2) is 60-70 ℃, and the mixing time is 5-15min.

21. The method for preparing the shampoo according to claim 18, wherein the mixing temperature in the step (3) is 40-50 ℃, and the mixing time is 5-15min.

22. The method for preparing the shampoo as claimed in claim 21, wherein the filtering in the step (3) is performed by using a filter cloth of 100-300 meshes.

23. The preparation method of the shampoo as claimed in claim 18, wherein the preparation method of the shampoo comprises the following steps: