CN111838678A - 一种增强免疫力的组合物及其应用 - Google Patents
一种增强免疫力的组合物及其应用 Download PDFInfo
- Publication number
- CN111838678A CN111838678A CN202010466477.4A CN202010466477A CN111838678A CN 111838678 A CN111838678 A CN 111838678A CN 202010466477 A CN202010466477 A CN 202010466477A CN 111838678 A CN111838678 A CN 111838678A
- Authority
- CN
- China
- Prior art keywords
- composition
- powder
- mass
- enhancing immunity
- probiotic bacteria
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 42
- 230000036039 immunity Effects 0.000 title claims abstract description 26
- 230000002708 enhancing effect Effects 0.000 title claims abstract description 21
- 239000000843 powder Substances 0.000 claims abstract description 32
- 239000006041 probiotic Substances 0.000 claims abstract description 24
- 235000018291 probiotics Nutrition 0.000 claims abstract description 24
- 230000000529 probiotic effect Effects 0.000 claims abstract description 21
- SQVRNKJHWKZAKO-UHFFFAOYSA-N beta-N-Acetyl-D-neuraminic acid Natural products CC(=O)NC1C(O)CC(O)(C(O)=O)OC1C(O)C(O)CO SQVRNKJHWKZAKO-UHFFFAOYSA-N 0.000 claims abstract description 12
- 241000894006 Bacteria Species 0.000 claims abstract description 11
- SQVRNKJHWKZAKO-PFQGKNLYSA-N N-acetyl-beta-neuraminic acid Chemical compound CC(=O)N[C@@H]1[C@@H](O)C[C@@](O)(C(O)=O)O[C@H]1[C@H](O)[C@H](O)CO SQVRNKJHWKZAKO-PFQGKNLYSA-N 0.000 claims abstract description 11
- 229920001503 Glucan Polymers 0.000 claims abstract description 10
- UPYKUZBSLRQECL-UKMVMLAPSA-N Lycopene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1C(=C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=C)CCCC2(C)C UPYKUZBSLRQECL-UKMVMLAPSA-N 0.000 claims abstract description 10
- JEVVKJMRZMXFBT-XWDZUXABSA-N Lycophyll Natural products OC/C(=C/CC/C(=C\C=C\C(=C/C=C/C(=C\C=C\C=C(/C=C/C=C(\C=C\C=C(/CC/C=C(/CO)\C)\C)/C)\C)/C)\C)/C)/C JEVVKJMRZMXFBT-XWDZUXABSA-N 0.000 claims abstract description 10
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 10
- OAIJSZIZWZSQBC-GYZMGTAESA-N lycopene Chemical compound CC(C)=CCC\C(C)=C\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C=C(/C)CCC=C(C)C OAIJSZIZWZSQBC-GYZMGTAESA-N 0.000 claims abstract description 10
- 229960004999 lycopene Drugs 0.000 claims abstract description 10
- 235000012661 lycopene Nutrition 0.000 claims abstract description 10
- 239000001751 lycopene Substances 0.000 claims abstract description 10
- ZCIHMQAPACOQHT-ZGMPDRQDSA-N trans-isorenieratene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/c1c(C)ccc(C)c1C)C=CC=C(/C)C=Cc2c(C)ccc(C)c2C ZCIHMQAPACOQHT-ZGMPDRQDSA-N 0.000 claims abstract description 10
- 235000003145 Hippophae rhamnoides Nutrition 0.000 claims abstract description 9
- 240000008397 Ganoderma lucidum Species 0.000 claims abstract description 6
- 235000001637 Ganoderma lucidum Nutrition 0.000 claims abstract description 6
- 240000000950 Hippophae rhamnoides Species 0.000 claims abstract 5
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 11
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 11
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 11
- 241001134770 Bifidobacterium animalis Species 0.000 claims description 6
- 229940118852 bifidobacterium animalis Drugs 0.000 claims description 6
- 241000186000 Bifidobacterium Species 0.000 claims description 4
- 235000013402 health food Nutrition 0.000 claims description 3
- 206010022000 influenza Diseases 0.000 abstract description 10
- 239000003814 drug Substances 0.000 abstract description 6
- 229940079593 drug Drugs 0.000 abstract description 6
- 230000036737 immune function Effects 0.000 abstract description 6
- 208000024891 symptom Diseases 0.000 abstract description 6
- 210000002865 immune cell Anatomy 0.000 abstract description 5
- 241000222336 Ganoderma Species 0.000 abstract description 4
- 230000000295 complement effect Effects 0.000 abstract description 4
- 230000002045 lasting effect Effects 0.000 abstract description 4
- 230000002829 reductive effect Effects 0.000 abstract description 4
- 244000052616 bacterial pathogen Species 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 235000016709 nutrition Nutrition 0.000 abstract description 3
- 230000003213 activating effect Effects 0.000 abstract description 2
- 229940088592 immunologic factor Drugs 0.000 abstract description 2
- 239000000367 immunologic factor Substances 0.000 abstract description 2
- 230000002401 inhibitory effect Effects 0.000 abstract description 2
- 230000003871 intestinal function Effects 0.000 abstract description 2
- 230000035764 nutrition Effects 0.000 abstract description 2
- 230000001105 regulatory effect Effects 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 11
- 241000229143 Hippophae Species 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 230000000694 effects Effects 0.000 description 6
- 241000699670 Mus sp. Species 0.000 description 5
- 210000000822 natural killer cell Anatomy 0.000 description 5
- 210000000952 spleen Anatomy 0.000 description 5
- 235000003935 Hippophae Nutrition 0.000 description 4
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- 230000002496 gastric effect Effects 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 241000699666 Mus <mouse, genus> Species 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 230000006870 function Effects 0.000 description 3
- 210000000987 immune system Anatomy 0.000 description 3
- 238000001727 in vivo Methods 0.000 description 3
- 230000000242 pagocytic effect Effects 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- 244000038022 Chenopodium capitatum Species 0.000 description 2
- 235000004391 Chenopodium capitatum Nutrition 0.000 description 2
- 239000012981 Hank's balanced salt solution Substances 0.000 description 2
- 102000015696 Interleukins Human genes 0.000 description 2
- 108010063738 Interleukins Proteins 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 206010057249 Phagocytosis Diseases 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 239000012636 effector Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 210000001035 gastrointestinal tract Anatomy 0.000 description 2
- 210000004185 liver Anatomy 0.000 description 2
- 210000001165 lymph node Anatomy 0.000 description 2
- 230000003287 optical effect Effects 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 230000008782 phagocytosis Effects 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 229910000029 sodium carbonate Inorganic materials 0.000 description 2
- 239000003053 toxin Substances 0.000 description 2
- 231100000765 toxin Toxicity 0.000 description 2
- OYHQOLUKZRVURQ-NTGFUMLPSA-N (9Z,12Z)-9,10,12,13-tetratritiooctadeca-9,12-dienoic acid Chemical compound C(CCCCCCC\C(=C(/C\C(=C(/CCCCC)\[3H])\[3H])\[3H])\[3H])(=O)O OYHQOLUKZRVURQ-NTGFUMLPSA-N 0.000 description 1
- 241000901050 Bifidobacterium animalis subsp. lactis Species 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 102000014150 Interferons Human genes 0.000 description 1
- 108010050904 Interferons Proteins 0.000 description 1
- 241000581650 Ivesia Species 0.000 description 1
- 239000012980 RPMI-1640 medium Substances 0.000 description 1
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- 108060008682 Tumor Necrosis Factor Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- 210000003651 basophil Anatomy 0.000 description 1
- 235000021028 berry Nutrition 0.000 description 1
- 229940009289 bifidobacterium lactis Drugs 0.000 description 1
- 210000001772 blood platelet Anatomy 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 230000003915 cell function Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000016396 cytokine production Effects 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 210000003162 effector t lymphocyte Anatomy 0.000 description 1
- 210000003979 eosinophil Anatomy 0.000 description 1
- -1 etc. Chemical class 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002212 flavone derivatives Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 210000003630 histaminocyte Anatomy 0.000 description 1
- 230000005965 immune activity Effects 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 229940099472 immunoglobulin a Drugs 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000005550 inflammation mediator Substances 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 229940079322 interferon Drugs 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000002864 mononuclear phagocyte Anatomy 0.000 description 1
- 210000000440 neutrophil Anatomy 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000004792 oxidative damage Effects 0.000 description 1
- 210000002741 palatine tonsil Anatomy 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 210000001539 phagocyte Anatomy 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- SQVRNKJHWKZAKO-OQPLDHBCSA-N sialic acid Chemical compound CC(=O)N[C@@H]1[C@@H](O)C[C@@](O)(C(O)=O)OC1[C@H](O)[C@H](O)CO SQVRNKJHWKZAKO-OQPLDHBCSA-N 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 210000001541 thymus gland Anatomy 0.000 description 1
- 102000003390 tumor necrosis factor Human genes 0.000 description 1
- 241000712461 unidentified influenza virus Species 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L19/00—Products from fruits or vegetables; Preparation or treatment thereof
- A23L19/09—Mashed or comminuted products, e.g. pulp, purée, sauce, or products made therefrom, e.g. snacks
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/125—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/20—Reducing nutritive value; Dietetic products with reduced nutritive value
- A23L33/21—Addition of substantially indigestible substances, e.g. dietary fibres
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
本发明公开了一种增强免疫力的组合物，其包括如下组分：益生菌菌粉、沙棘粉、灵芝提取物、酵母葡聚糖、番茄红素和N‑乙酰神经氨酸；按质量比计，益生菌菌粉:沙棘粉:灵芝提取物:酵母葡聚糖:番茄红素:N‑乙酰神经氨酸＝1:(3～5):(8～12):(8～12):(2～4):(0.4～0.6)。本发明组合物的各组分之间具有较好的互补互利作用，从而使组合物可更好地同时调节不同的免疫因子，从多方面发挥作用来增强机体免疫功能，包括为机体提供丰富的营养、改善肠道功能、抑制病原菌生长、活化免疫细胞和调节机体免疫功能等，能减少流感发生的次数、症状的持续天数和药物治疗的天数。
Description
技术领域
本发明属于保健食品技术领域，尤其涉及一种增强免疫力的组合物及其应用。
背景技术
人体的免疫系统是人体抵御病原菌侵犯最重要的保卫系统，该系统由免疫器官(骨髓、胸腺、脾脏、淋巴结、扁桃体、小肠集合淋巴结、阑尾等)、免疫细胞(淋巴细胞、单核吞噬细胞、中性粒细胞、嗜碱粒细胞、嗜酸粒细胞、肥大细胞、血小板)、以及免疫分子(补体、免疫球蛋白、干扰素、白细胞介素、肿瘤坏死因子等细胞因子)等组成。免疫器官、免疫细胞及免疫分子分布在全身，形成一个相互制约的网络，主宰着人体的免疫功能——抵抗细菌、病毒入侵；清除体内损伤变性及衰老的细胞；清除体内代谢废物；维持体内环境的稳定。
流行性感冒(简称流感)是由流感病毒引起的急性呼吸道传染病，可周期性地引起大流行。而增强免疫力在抵抗流感的过程中起到了非常重要的作用，免疫力较强的人相对来说更加不容易被感染或恢复得更快。
发明内容
基于此，本发明的目的在于提供一种增强免疫力的组合物，该组合物具有较高的营养价值，能增强人体免疫力，减少流感发生的次数、症状的持续天数和药物治疗的天数。
为实现其目的，本发明采取的技术方案如下：
一种增强免疫力的组合物，其包括如下组分：益生菌菌粉、沙棘粉、灵芝提取物、酵母葡聚糖、番茄红素和N-乙酰神经氨酸。
优选地，所述组合物中各组分的质量比为，益生菌菌粉:沙棘粉:灵芝提取物:酵母葡聚糖:番茄红素:N-乙酰神经氨酸＝1:(3～5):(8～12):(8～12):(2～4):(0.4～0.6)。
最优选地，所述组合物中各组分的质量比为，益生菌菌粉:沙棘粉:灵芝提取物:酵母葡聚糖:番茄红素:N-乙酰神经氨酸＝1:4:10:10:3:0.5。
优选地，所述益生菌菌粉中的益生菌选自动物双歧杆菌(Bifidobacteriumlactis)和/或植物乳杆菌(Lactobacillus plantarum)。所述益生菌的制备方法为本领域常规制备方法，或可通过商业购买获得。
更优选地，所述益生菌菌粉中的益生菌选自动物双歧杆菌和植物乳杆菌。
优选地，所述益生菌菌粉中，双歧杆菌的活菌数不低于50亿CFU/g，植物乳杆菌的活菌数不低于500亿CFU/g。
本发明还提供了所述增强免疫力的组合物的用途，包括其在保健食品中的应用。
本发明还提供了一种保健食品，其含有本发明所述的增强免疫力的组合物。
益生菌是对宿主(如动物或人类)有正面效益的活性微生物，可以通过刺激肠道内的免疫机能，将过低或过高的免疫活性调节至正常状态。
沙棘粉由沙棘果制成；沙棘果为黄色小浆果，其营养价值很高，富含多种维生素、脂肪酸、微量元素、亚油素、沙棘黄酮、超氧化物等活性物质和人体所需的各种氨基酸。
灵芝提取物可调节免疫系统的众多组成成分，例如免疫球蛋白A等，从而可改善免疫系统功能。
酵母葡聚糖能够刺激增强NK细胞、巨噬细胞等免疫细胞的吞噬活性，刺激释放白介素以增强人体抵抗有害毒素的能力，可以有效增强人体清除毒素的能力，提高人体免疫力。
番茄红素可活化免疫细胞，保护吞噬细胞免受自身的氧化损伤，促进T、B淋巴细胞增殖，刺激效应T细胞的功能，促进某些白介素产生及抑制炎症介质生成。
N-乙酰神经氨酸(又称唾液酸)可阻碍以其为受体的病原体侵染目标宿主细胞，同时还可促进植物乳杆菌和双歧杆菌的增殖，调整微生物群落变化，维持肠道健康，从而提高机体免疫力。
本发明以益生菌菌粉、沙棘粉、灵芝提取物、酵母葡聚糖、番茄红素和N-乙酰神经氨酸这几种组分以特定的配比复配制成组合物，在本发明提供的配比下，组合物的各组分之间具有较好的互补互利作用，从而使组合物可更好地同时调节不同的免疫因子，从多方面发挥作用来增强机体免疫功能，包括为机体提供丰富的营养、改善肠道功能、抑制病原菌生长、活化免疫细胞和调节机体免疫功能等。
本发明经试验研究发现，本发明的组合物具有增强免疫力的保健作用，能减少流感发生的次数、症状的持续天数和药物治疗的天数。
具体实施方式
为更好的说明本发明的目的、技术方案和优点，本发明通过下列实施例进一步说明。显然，下列实施例仅是本发明的一部分实施例，而不是全部的实施例。应理解，本发明实施例仅用于说明本发明的技术效果，而非用于限制本发明的保护范围。除非特别说明之外，实施例中所用原料均可通过市售购买获得。
实施例中的益生菌菌粉由动物双歧杆菌冻干粉和植物乳杆菌冻干粉按1:1的质量比混合而成；且益生菌菌粉中，动物双歧杆菌的活菌数不低于50亿CFU/g，植物乳杆菌的活菌数不低于500亿CFU/g。动物双歧杆菌冻干粉购自广州汇健生物科技有限公司，型号为：食品级20181015-46；植物乳杆菌冻干粉购自广州汇健生物科技有限公司，型号为：食品级SP180824。
实施例：
实验组1～3和对比组1～5的组合物由表1所示质量比的组分组成：
表1
组合物的制备方法为：按配方比例称取各原料组分，混合均匀，即可。
试验例1：
受试样品为上述实验组1～3和对比组1～5的组合物。体重为18～22克的实验小鼠，随机分为8组，每组15只。将待测样品灌胃小鼠，每天灌胃一次，连续灌胃30天，每次灌胃量为0.167g/(kg·d)。
(1)碳廓清试验：
小鼠尾静脉注射1:3稀释的印度墨汁，按每10g体重0.1mL计算，待墨汁注入，立即计时；墨汁注入后的2min、10min分别从内眦静脉丛取血20μL，并立即将其加到2mL 0.1％碳酸钠溶液中；用半自动生化分析仪在600nm波长处测光密度值，以碳酸钠溶液作空白对照；将小鼠处死，取肝脏和脾脏，用滤纸吸干脏器表面血污，称重；计算吞噬指数。结果如下表2所示。
吞噬指数计算公式如下:
κ＝(lgOD1-lgOD2)/(t2-t1)
α＝体重/(肝重+脾重)×κ1/3
表2
(2)组合物对小鼠NK细胞活性的影响：
无菌取脾，置于盛有适量无菌Hank’s液的研磨器中，轻轻将脾脏磨碎，制成细胞悬液。经200目筛网过滤，用Hank’s液洗2次，每次离心10min(1000r/min)，最后用RPMI1640完全培养液调整细胞浓度为2×107个/mL；取靶细胞和效应细胞各100μL(效靶比50:1)，加入U型96孔培养板中；靶细胞自然释放孔加靶细胞和培养液各100μL，靶细胞最大释放孔加靶细胞和1％NP40各100μL；上述各项均设三个复孔，于37℃、5％CO2培养箱中培养4h，然后将96孔培养板以1500r/min离心5min，每孔吸取上清100μL置平底96孔培养板中，同时加入LDH基质液100μL，反应3min，每孔加入1mol/L的HCl 30μL，在酶标仪490nm处测定光密度值(OD)。结果如下表3所示。
NK细胞活性(％)＝(反应孔OD-自然释放孔OD)/(最大释放孔OD-自然释放孔OD)×100
表3
由表2和表3可看出：相比于对比组1和对比组2，喂服实验组1～3组合物的小鼠的碳廓清能力(吞噬指数α)和NK细胞活性均有明显提高，说明组分之间的配比也会对组合物的功效产生影响，而以本发明提供的配比制成的组合物具有更好的增强免疫力功效。相比于对比组3、对比组4和对比组5，喂服对比组1、对比组2和实验组1～3的组合物的小鼠的碳廓清能力(吞噬指数α)和NK细胞活性均有明显提高，说明组合物的各组分之间发挥了互补互利作用，本发明配方的组合物可更好地增强小鼠免疫力。
试验例2：
本试验选取30个免疫力较低的成人志愿者作为受试人员，随机均分为3组，每组为10人，分别服用实验组1～3的组合物。服用的量为10.0g/人/次，每天服用1次，连续服用2个月。考察受试人员的流感发生次数、症状持续天数和药物治疗天数。结果如下表4所示。
表4
由表4可看出，受试人员服用本发明实验组1～3的组合物60d后，大部分受试人员的流感发生次数减少、流感症状持续天数减少以及需要药物治疗的天数减少。表明，本发明的组合物具有增强免疫力的保健作用，能减少流感发生的次数、症状的持续天数和药物治疗的天数。
最后应当说明的是，以上实施例仅用以说明本发明的技术方案而非对本发明保护范围的限制，尽管参照较佳实施例对本发明作了详细说明，本领域的普通技术人员应当理解，可以对本发明的技术方案进行修改或者等同替换，而不脱离本发明技术方案的实质和范围。
Claims (8)
1.一种增强免疫力的组合物，其特征在于，包括如下组分：益生菌菌粉、沙棘粉、灵芝提取物、酵母葡聚糖、番茄红素和N-乙酰神经氨酸。
2.如权利要求1所述的增强免疫力的组合物，其特征在于，所述组合物中各组分的质量比为，益生菌菌粉:沙棘粉:灵芝提取物:酵母葡聚糖:番茄红素:N-乙酰神经氨酸＝1:(3～5):(8～12):(8～12):(2～4):(0.4～0.6)。
3.如权利要求1所述的增强免疫力的组合物，其特征在于，所述组合物中各组分的质量比为，益生菌菌粉:沙棘粉:灵芝提取物:酵母葡聚糖:番茄红素:N-乙酰神经氨酸＝1:4:10:10:3:0.5。
4.如权利要求1～3任一项所述的增强免疫力的组合物，其特征在于，所述益生菌菌粉中的益生菌选自动物双歧杆菌和/或植物乳杆菌。
5.如权利要求4所述的增强免疫力的组合物，其特征在于，所述益生菌菌粉中的益生菌选自动物双歧杆菌和植物乳杆菌。
6.如权利要求4所述的增强免疫力的组合物，其特征在于，所述益生菌菌粉中，双歧杆菌的活菌数不低于50亿CFU/g，植物乳杆菌的活菌数不低于500亿CFU/g。
7.如权利要求1～6任一项所述的增强免疫力的组合物在保健食品中的应用。
8.一种保健食品，其特征在于，含有如权利要求1～6任一项所述的增强免疫力的组合物。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010466477.4A CN111838678A (zh) | 2020-05-27 | 2020-05-27 | 一种增强免疫力的组合物及其应用 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010466477.4A CN111838678A (zh) | 2020-05-27 | 2020-05-27 | 一种增强免疫力的组合物及其应用 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN111838678A true CN111838678A (zh) | 2020-10-30 |
Family
ID=72985291
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010466477.4A Pending CN111838678A (zh) | 2020-05-27 | 2020-05-27 | 一种增强免疫力的组合物及其应用 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111838678A (zh) |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101485463A (zh) * | 2009-03-03 | 2009-07-22 | 南京中科集团股份有限公司 | 一种具有多种保健功效的天然产品组方 |
| CN101569414A (zh) * | 2008-04-29 | 2009-11-04 | 安琪酵母股份有限公司 | 一种富含酵母硒和酵母葡聚糖的组合物 |
| CN105687277A (zh) * | 2016-03-17 | 2016-06-22 | 天津元恒德科技有限公司 | 一种提高免疫力的组合物及应用 |
| CN109528807A (zh) * | 2019-01-15 | 2019-03-29 | 斯潘思生命科技(武汉)有限公司 | 一种增强人体免疫力的中药组合物及其制备方法和应用 |
| CN109645473A (zh) * | 2019-01-16 | 2019-04-19 | 汤臣倍健股份有限公司 | 一种改善胃肠道和增强免疫力的组合物及其应用 |
| CN109744446A (zh) * | 2019-02-19 | 2019-05-14 | 杭州海王生物工程有限公司 | 一种含n-乙酰神经氨酸的乳清蛋白类固体饮料及其制备方法 |
| CN109757686A (zh) * | 2019-02-27 | 2019-05-17 | 东莞百润生物科技有限公司 | 一种含n-乙酰神经氨酸的蛋白粉食品及其制备方法 |
| CN109938340A (zh) * | 2018-12-28 | 2019-06-28 | 南京中科药业有限公司 | 一种具有增强免疫力功效的复合益生菌组合物 |
| CN110122724A (zh) * | 2019-05-27 | 2019-08-16 | 九饮堂生物科技有限公司 | 一种具有保健功效的固体饮料及其制备方法 |
| CN111184072A (zh) * | 2020-03-02 | 2020-05-22 | 上海育博营养食品有限公司 | 一种有利于增强免疫、提高抵抗力的奶粉及其生产方法 |
-
2020
- 2020-05-27 CN CN202010466477.4A patent/CN111838678A/zh active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101569414A (zh) * | 2008-04-29 | 2009-11-04 | 安琪酵母股份有限公司 | 一种富含酵母硒和酵母葡聚糖的组合物 |
| CN101485463A (zh) * | 2009-03-03 | 2009-07-22 | 南京中科集团股份有限公司 | 一种具有多种保健功效的天然产品组方 |
| CN105687277A (zh) * | 2016-03-17 | 2016-06-22 | 天津元恒德科技有限公司 | 一种提高免疫力的组合物及应用 |
| CN109938340A (zh) * | 2018-12-28 | 2019-06-28 | 南京中科药业有限公司 | 一种具有增强免疫力功效的复合益生菌组合物 |
| CN109528807A (zh) * | 2019-01-15 | 2019-03-29 | 斯潘思生命科技(武汉)有限公司 | 一种增强人体免疫力的中药组合物及其制备方法和应用 |
| CN109645473A (zh) * | 2019-01-16 | 2019-04-19 | 汤臣倍健股份有限公司 | 一种改善胃肠道和增强免疫力的组合物及其应用 |
| CN109744446A (zh) * | 2019-02-19 | 2019-05-14 | 杭州海王生物工程有限公司 | 一种含n-乙酰神经氨酸的乳清蛋白类固体饮料及其制备方法 |
| CN109757686A (zh) * | 2019-02-27 | 2019-05-17 | 东莞百润生物科技有限公司 | 一种含n-乙酰神经氨酸的蛋白粉食品及其制备方法 |
| CN110122724A (zh) * | 2019-05-27 | 2019-08-16 | 九饮堂生物科技有限公司 | 一种具有保健功效的固体饮料及其制备方法 |
| CN111184072A (zh) * | 2020-03-02 | 2020-05-22 | 上海育博营养食品有限公司 | 一种有利于增强免疫、提高抵抗力的奶粉及其生产方法 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| RU2370532C2 (ru) | Способ ферментации растительного материала и культивирования бактерий, экстракт ферментированного растительного материала, порошок экстракта ферментированного растительного материала и их применение | |
| CN109576185B (zh) | 一种具有抗流感能力的益生菌混合制剂及其应用 | |
| CN114736875B (zh) | 一种鸭疫里默氏杆菌噬菌体、其噬菌体组合物及其应用 | |
| CN116891810A (zh) | 具有降血糖功效的益生菌组合物、益生菌益生元复合制剂 | |
| JP4132635B2 (ja) | 未失活酵素強化組成物 | |
| KR20180041563A (ko) | 장 및 뇌건강 개선 기능성 반려동물 사료첨가제 | |
| CN103083510A (zh) | 一种提高禽免疫功能的中药口服液及其制备方法 | |
| CN114052116B (zh) | 一种益生菌植物提取物复合生物制剂及其制备方法和应用 | |
| CN111838678A (zh) | 一种增强免疫力的组合物及其应用 | |
| CN111165651A (zh) | 一种添加剂预混合饲料及其应用 | |
| AU2017205019B2 (en) | Glycosaminoglycan ester and application thereof | |
| CN111195298B (zh) | 一种调节肠道菌群和防治食物过敏的组合物及其制备方法 | |
| CN101595945A (zh) | 一种促生产、增强动物抗病能力的饲料添加剂 | |
| Kazemi et al. | Evaluation the effect of royal jelly on the growth of two members of gut microbiota; Bacteroides fragillis and Bacteroides thetaiotaomicron. | |
| CN107029214A (zh) | 一种调节肠道微生物的组合物及其制备方法 | |
| CN1171631C (zh) | 含香菇菌丝体提取物的γδT细胞免疫活性增强剂 | |
| KR20030021298A (ko) | 동물용 면역증진 및 정장용 조성물 | |
| CN1401390A (zh) | 一种免疫调节药物——抗体生成素及其制备方法和应用 | |
| CN118389372B (zh) | 一种具有减肥和改善腹泻功能的植物乳植杆菌 | |
| CN110237113B (zh) | 一种抗大肠杆菌与球虫混合感染的植物提取物添加剂及应用 | |
| CN117357627B (zh) | 一种提高家禽机体免疫力的复合药物及其制备方法和应用 | |
| CN111544570B (zh) | 一种添加抗菌肽的抗病毒制剂及其制备方法 | |
| CN113368155A (zh) | 连翘益生菌发酵物及其在制备防治畜禽病毒性疾病药物中的应用 | |
| CN102228679B (zh) | 一种鸡干扰素γ组合药物与应用 | |
| An et al. | Effect of the supplementation of pig skin collagen on growth performance, organ weight, blood characteristics and intestinal microbiota in broilers |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20220328 Address after: 310000 room 1103-05, building 2, No. 371, Xingxing Road, economic and Technological Development Zone, Xiaoshan District, Hangzhou City, Zhejiang Province Applicant after: Zhongke Chenxing (Hangzhou) Technology Co.,Ltd. Address before: 230000 No. 350 Shushan Lake Road, Hefei City, Anhui Province Applicant before: HEFEI INSTITUTES OF PHYSICAL SCIENCE, CHINESE ACADEMY OF SCIENCES |
|
| TA01 | Transfer of patent application right | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20201030 |
|
| RJ01 | Rejection of invention patent application after publication |