CN111372943A - 腺病毒及其用途 - Google Patents

腺病毒及其用途 Download PDF

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CN111372943A
CN111372943A CN201880071256.8A CN201880071256A CN111372943A CN 111372943 A CN111372943 A CN 111372943A CN 201880071256 A CN201880071256 A CN 201880071256A CN 111372943 A CN111372943 A CN 111372943A
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T·G·乌伊尔
S·罗伊
S·卡恩
J·H·H·V·屈斯泰
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Abstract

本文提供了腺病毒核酸序列和包含所述核酸序列的腺病毒载体。所提供的腺病毒载体可用于在受试者中诱导保护性免疫应答。

Description

腺病毒及其用途
技术领域
本发明涉及生物技术。更具体地说，涉及腺病毒载体诸如复制缺陷型腺病毒载体在宿主中递送抗原并引发免疫应答的领域和用途。
背景技术
重组腺病毒载体广泛应用于基因治疗应用和疫苗。基于AdV-5载体的疫苗已显示出在多种动物模型中引发强有力的和保护性的免疫应答(参见例如WO 2001/02607；WO2002/22080；Shiver等人,Nature[自然]415:331(2002)；Letvin等人,Ann.Rev.Immunol.[免疫学年鉴]20:73(2002)；Shiver和Emini,Ann.Rev.Med.[医学年鉴]55:355(2004))。然而，基于重组AdV-5载体的疫苗的效用将可能受到人群中AdV-5特异性中和抗体(NAb)的高血清阳性率的限制。在小鼠、恒河猴和人的研究中，抗AdV-5免疫的存在已经显示出基本上抑制了基于AdV-5的疫苗的免疫原性。
一种在先前已被最常见的人腺病毒(例如AdV-5)感染或治疗的个体中规避预先存在的免疫性的有前景的策略涉及开发来自腺病毒血清型的重组载体，这些腺病毒血清型未遇到这种预先存在的免疫性。一种这样的策略是基于使用非人猿猴腺病毒，因为这些腺病毒通常不感染人并且在人样品中表现出低血清阳性率。非人猿猴腺病毒可应用于人类用途，因为已表明这些病毒可在体外感染人细胞(WO 2003/000283、WO 2004/037189)。
因此，本领域需要可大量生产的、在宿主中不会遇到预先存在的免疫、但仍具有免疫原性并能够诱导针对由插入载体中的异源核酸编码的抗原的强烈免疫应答的替代性腺病毒载体。
发明内容
提供了编码六邻体多肽的分离的核酸序列。在某些实施例中，六邻体多肽或其功能衍生物包括含六邻体高变区多肽，该含六邻体高变区多肽具有SEQ ID NO:1的氨基酸序列或在其全长上与SEQ ID NO:1的氨基酸序列具有至少95％同一性的氨基酸序列。
在某些实施例中，六邻体多肽包括具有SEQ ID NO:1的氨基酸序列的含六邻体高变区多肽。在某些实施例中，六邻体多肽或其功能衍生物包含BLY6六邻体多肽的氨基酸序列(SEQ ID NO:2)或在其全长上与SEQ ID NO:2的氨基酸序列具有至少95％同一性的氨基酸序列。
还提供了编码纤维多肽或其功能衍生物的分离的核酸序列。在某些实施例中，纤维多肽包含以下至少一者：包含SEQ ID NO:10的氨基酸序列的纤维突结(knob)多肽序列；包含SEQ ID NO:11的氨基酸序列的纤维轴(shaft)多肽序列；以及包含SEQ ID NO:12的氨基酸序列的纤维尾多肽序列。在某些实施例中，纤维多肽或其功能衍生物包含BLY6纤维多肽的氨基酸序列(SEQ ID NO:3)或在其全长上与SEQ ID NO:3的氨基酸序列具有至少95％同一性的氨基酸序列。
本发明的实施例还包括由本发明的纤维核酸序列和六邻体核酸序列编码的分离的纤维多肽和六邻体多肽。
本文进一步提供了分离的核酸，这些分离的核酸包含编码本文披露的六邻体多肽中的至少一种的六邻体核酸序列，以及编码本文披露的纤维多肽中的至少一种的纤维核酸序列。在某些实施例中，本文提供了包含本文所述的分离的核酸的载体。在一个实施例中，该载体是病毒载体。在另一个实施例中，该载体是表达载体。在一个优选的实施例中，该载体是腺病毒载体。更优选地，该载体进一步包含转基因。
还提供了包含本文所述的载体的重组细胞。这样的细胞可以用于重组蛋白生产、重组蛋白表达，或载体或病毒颗粒的生产。还提供了生产载体的方法。这些方法包括(a)使本文披露的重组细胞在用于生产载体的条件下生长；以及(b)从该重组细胞中分离该载体。
在某些实施例中，提供了包含本文披露的载体的免疫原性组合物。还提供了在有需要的受试者中诱导免疫应答的方法，这些方法包括向该受试者施用本文披露的免疫原性组合物。
在某些实施例中，提供了腺病毒载体，这些腺病毒载体包含(a)至少一个转基因；以及(b)根据本发明的实施例的编码六邻体多肽的核酸序列。六邻体多肽可以例如包括含六邻体高变区多肽，该含六邻体高变区多肽具有SEQ ID NO:1的氨基酸序列或在其全长上与SEQ ID NO:1的氨基酸序列具有至少95％同一性的氨基酸序列。六邻体多肽可以例如包含具有SEQ ID NO:1的氨基酸序列的氨基酸序列。在某些实施例中，六邻体多肽包含BLY6六邻体多肽的氨基酸序列(SEQ ID NO:2)或在其全长上与SEQ ID NO:2的氨基酸序列具有至少95％同一性的氨基酸序列。
在某些实施例中，提供了腺病毒载体，这些腺病毒载体包含(a)至少一个转基因；以及(b)根据本发明的实施例的编码纤维多肽的核酸序列。在某些实施例中，纤维多肽可以包含以下至少一者：包含SEQ ID NO:10的氨基酸序列的纤维突结多肽序列；包含SEQ IDNO:11的氨基酸序列的纤维轴多肽序列；以及包含SEQ ID NO:12的氨基酸序列的纤维尾多肽序列。在某些实施例中，纤维多肽或其功能衍生物包含BLY6纤维多肽的氨基酸序列(SEQID NO:3)或在其全长上与SEQ ID NO:3的氨基酸序列具有至少95％同一性的氨基酸序列。本发明的实施例还包括腺病毒载体，这些腺病毒载体包含：(a)至少一个转基因；(b)根据本发明的实施例的编码六邻体多肽的核酸序列；以及(c)根据本发明的实施例的编码纤维多肽的核酸序列。
在某些实施例中，本文提供的腺病毒载体是复制缺陷型腺病毒载体(rAd)。在一个实施例中，腺病毒载体可以包含E1缺失。在某些实施例中，本文提供的腺病毒载体可进一步包含E3缺失。腺病毒载体可以是包含来自一种或多种猿猴腺病毒(SAdV)诸如黑猩猩腺病毒(例如ChAd3)、大猩猩腺病毒或恒河猴腺病毒(例如，rhAd51、rhAd52或rhAd53)的腺病毒核酸序列的猿猴腺病毒载体。腺病毒载体可以是包含来自一种或多种人腺病毒(例如，hAdV-4、hAdV-5、hAdV-26、hAdV-35)的腺病毒序列的人腺病毒载体。优选地，腺病毒载体是包含一个或多个人腺病毒核酸序列的嵌合腺病毒载体。人腺病毒核酸序列可以例如来自人腺病毒-4(hAdV-4)、人腺病毒-5(hAdV-5)、人腺病毒-26(hAdV-26)或人腺病毒-35(hAdV-35)。腺病毒载体可以例如包含人腺病毒-5(hAdV-5)E4 orf6和orf6/7。
在某些实施例中，转基因位于反向末端重复序列(ITR)附近。在某些实施例中，转基因位于E1缺失处或附近、E3缺失处或附近、和/或ITR处或附近，例如在E4区域与右ITR(RITR)之间。
在某些实施例中，本文提供的腺病毒载体包含SEQ ID NO:13或SEQ ID NO:14的核酸序列。
还提供了包含本文所述的腺病毒载体和药学上可接受的载剂的免疫原性组合物或疫苗。进一步提供了用于在有需要的受试者中诱导免疫应答的方法。这些方法包括向该受试者施用本文披露的疫苗。进一步提供了生产疫苗的方法。这些方法包括将本文披露的腺病毒载体与药学上可接受的载剂组合。
附图说明
当结合附图阅读时，将更好地理解前述发明内容以及本申请的优选实施例的以下详细描述。然而，应当理解，本申请不限于附图中所示的精确实施例。
图1显示由BLY6.FLuc和Ad49.FLuc诱导的细胞和体液免疫应答。图1A显示实验设置。图1B显示由Ad26.FLuc、BLY6.FLuc和Ad49.FLuc诱导的针对载体编码的抗原(即，Fluc，萤火虫萤光素酶)的细胞免疫应答，如通过干扰素γ(IFN-γ)ELISPOT分析所测定的。y轴显示每106个脾细胞的点形成单位(SFU)的数量，虚线表示培养基刺激的95％百分位数。
图2显示由BLY6.RSVF-2A-GLuc诱导的细胞和体液免疫应答。图2A显示实验设置。图2B显示在用Ad26.RSVF-2A-GLuc或用BLY6.RSVF-2A-GLuc以三种不同的浓度(108、109和1010vp)或用Ad26.FLuc或BLY6.FLuc以1010vp免疫后八周时进行的病毒中和试验(VNA)的结果的图。图2C显示免疫后由Ad26.RSVF-2A-GLuc和BLY6.RSVF-2A-GLuc诱导的细胞免疫应答，如通过IFN-γELISPOT分析所测定的。图2D显示由免疫后8周时免疫小鼠血清中的Ad26.RSVF-2A-GLuc或BLY6.RSVF-2A-GLuc诱导的RSVF特异性IgG结合抗体的图。
图3显示在用腺病毒载体Ad35、Ad26、Ad49、Ad5、Ad4和BLY6免疫的小鼠中诱导的同源和异源腺病毒中和滴度。
图4显示Ad35、Ad26、Ad5和BLY6在200个来自年龄为18-55岁、生活在美国(US)和欧盟(EU)的成人的人类群组血清样品中的血清阳性率。将这些血清中针对每种载体测得的中和滴度分成四个类别(<16(阴性)、16至300、300至1,000、1000至4000和>4000)，如在所示的图表中表示的。
图5显示质粒pBLY6.dE1.dE3(SEQ ID NO:8)的示意图。
图6显示质粒pBLY6.dE1.dE3.5IXp(SEQ ID NO:9)的示意图。
图7显示BLY6六邻体多肽(SEQ ID NO:2)和SAdV-30-1六邻体多肽(SEQ ID NO:4)序列的比对。
图8显示新型载体BLY6.Fluc在生产细胞系sPER.C6中的生产率。
具体实施方式
本披露至少部分地基于分离和鉴定分入腺病毒E种的新大猩猩腺病毒分离株，以及构建和评价包含编码所述大猩猩腺病毒的六邻体和纤维多肽的可变区的核酸的疫苗载体。这些腺病毒载体能够引发免疫应答，而且在人体中具有低血清阳性率。这些腺病毒载体可以被配制成疫苗并用于诱导针对感兴趣的特异性抗原的保护性免疫。
在背景技术和整个说明书中引用或描述了各种出版物、文章和专利；这些参考文献各自通过援引以其全文并入本文。包括在本说明书中的对文件、法案、材料、装置、制品等的讨论用于提供本发明的背景的目的。这种讨论不承认任何或所有这些事项形成关于所披露或要求保护的任何发明的现有技术的一部分。
除非另外定义，否则本文所用的所有科学和技术术语具有如本发明所属领域的普通技术人员通常理解的相同含义。否则，本文所用的某些术语具有说明书中阐述的含义。
必须注意，除非上下文另外明确规定，否则如本文和所附权利要求中所用，单数形式“一个/一种”和“该”包括复数指示物。
除非另有说明，否则任何数值，诸如本文所述的浓度或浓度范围，应被理解为在所有情况下都被术语“约”修饰。因此，数值典型地包括列举值±10％。例如，1mg/mL的浓度包括0.9mg/mL至1.1mg/mL。同样地，1％至10％(w/v)的浓度范围包括0.9％(w/v)至11％(w/v)。如本文所用，数值范围的使用明确地包括所有可能的子范围，该范围内的所有单个数值，包括值的这些范围内的整数和分数，除非上下文另有明确指示。
除非另外指明，否则在一系列要素前面的术语“至少”应被理解为指该系列中的每一个要素。本领域技术人员将认识到或能够仅使用常规实验便确定本文所述的本发明的具体实施例的许多等同方案。这些等同方案也旨在包括在本发明中。
如本文所用，术语“包括”、“包含”、“具有”、“含有”或它们的任何其他变化，将被理解为意味着包括所述整数或整数组，而不排除任何其他整数或整数组，并且旨在是非排他性的或开放性的。例如，包含元件的清单的组合物、混合物、工艺、方法、物品或设备不一定限制于仅那些元件，而可以包括不是此类组合物、混合物、工艺、方法、物品或设备明确列出的或固有的其他元件。此外，除非明确地说明是相反的，否则“或”是指包括在内的或，而不是指排他性的或。例如，以下项中的任一者满足条件A或B：A是真(或存在)并且B是假(或不存在)，A是假(或不存在)并且B是真(或存在)，以及A和B二者都是真(或存在)。
如本文所用，多个引用的要素之间的连接术语“和/或”应被理解为涵盖单独的和组合的选项两者。例如，当两种要素由和/或连接时，第一选项是指在不具有第二要素的情况下第一要素的应用。第二选项是指在不具有第一要素的情况下第二要素的应用。第三选项是指第一要素和第二要素一起的应用。这些选项中的任一个应被理解为属于该含义之内，并且因此满足如本文所用的术语“和/或”的要求。这些选项中的多于一个的同时应用也应被理解为属于该含义之内，并且因此满足术语“和/或”的要求。
如本文所用，贯穿说明书和权利要求书所用的术语“由……组成”指示包括任何列举的整数或整数组，但不能将另外的整数或整数组添加至指定的方法、结构或组合物。
如本文所用，贯穿说明书和权利要求书所用的术语“基本上由……组成”指示包括任何列举的整数或整数组，并且任选地包括不实质性改变所指定的方法、结构或组合物的基本或新颖特性的任何列举的整数或整数组。参见M.P.E.P.§2111.03。
如本文所用，“受试者”是指任何动物，优选哺乳动物，最优选人，其将或已经通过根据本发明实施例的方法接种。如本文所用的术语“哺乳动物”涵盖任何哺乳动物。哺乳动物的实例包括但不限于牛、马、羊、猪、猫、狗、小鼠、大鼠、兔、豚鼠、猴、人等，更优选地是人。
词语“右”、“左”、“下”和“上”表示所参考的附图中的方向。
应当理解的是，当提及优选的发明的组分的规格或特征时，在此使用的术语“约”、“大约”、“总体上”、“基本上”、以及类似术语，指示描述的规格/特征不具有严格的界限或参数，并且并不从其排除功能上相同或相似的微小变化，如本领域普通技术人员将理解的那样。在最低程度上，包括数值参数的此类引用将包括将不改变最低有效位数的变化，使用本领域接受的数学的和产业的原理(例如化整误差、测量误差或其他系统误差、制造公差等)。
在两个或更多个核酸或多肽序列(例如六邻体和纤维多肽以及编码它们的多核苷酸)的上下文中，术语“相同的”或“同一性”百分比是指当进行比较和比对以获得最大对应性时，两个或更多个序列或子序列是相同的或具有指定百分比的相同氨基酸残基或核苷酸，如使用以下序列比较算法之一或通过目视检查所测量的。
对于序列比较，典型地，一个序列作为参考序列，测试序列与其进行比较。当使用序列比较算法时，将测试序列和参考序列输入计算机；如果需要，指定子序列坐标；以及指定序列算法程序参数。然后，序列比较算法基于指定的程序参数计算测试序列相对于参考序列的序列同一性百分比。
用于比较的序列的最佳比对可以例如通过Smith&Waterman,Adv.Appl.Math.[应用数学进展]2:482(1981)的局部同源性算法、通过Needleman&Wunsch,J.Mol.Biol.[分子生物学杂志]48:443(1970)的同源性比对算法、通过Pearson&Lipman,Proc.Nat’l.Acad.Sci.USA[美国国家科学院院刊]85:2444(1988)的类似性搜索方法、通过这些算法的计算机化实现形式(GAP、BESTFIT、FASTA和TFASTA，威斯康星遗传学软件包(WisconsinGenetics Software Package),遗传学计算机课题组(Genetics Computer Group)，威斯康星州麦迪逊科学大道575号(575Science Dr.,Madison,WI))、或通过目视检查(一般参见Current Protocols in Molecular Biology[分子生物学实验室指南],F.M.Ausubel等人编,Current Protocols[实验室指南],格林出版联合公司(Greene PublishingAssociates,Inc.)和约翰威利父子公司(John Wiley&Sons,Inc.)的合资公司,(1995年增刊)(Ausubel))来进行。
适于确定序列同一性百分比和序列相似性百分比的算法的实例是BLAST和BLAST2.0算法，这些算法描述于Altschul等人(1990)J.Mol.Biol.[分子生物学杂志]215:403-410和Altschul等人(1997)Nucleic Acids Res.[核酸研究]25:3389-3402。用于进行BLAST分析的软件可通过国家生物技术信息中心(National Center for BiotechnologyInformation)公开获得。该算法涉及首先通过鉴定查询序列中长度为W的短字来鉴定高得分序列对(HSP)，当与数据库序列中相同长度的字进行比对时，这些序列对匹配或满足一些正值阈值得分T。T被称为邻近字分值阈值(Altschul等人，同上)。这些初始的邻近字命中将作为种子，以用于启动搜索以发现包含它们的更长的HSP。然后，只要累积比对得分可以增加，字命中就沿着每个序列在两个方向上延伸。
对于核苷酸序列，累积得分使用参数M(一对匹配残基的奖励得分；总是>0)和N(错配残基的罚分；总是<0)来计算。对于氨基酸序列，使用评分矩阵来计算累积得分。当存在以下情况时，停止字命中在每个方向上的扩展：累积比对得分从其最大获得值下降数量X；由于一个或多个负得分残基比对的累积，累积得分变为零或更低；或者到达任一序列的末端。BLAST算法参数W、T和X决定比对的灵敏度和速度。BLASTN程序(用于核苷酸序列)使用11的字长(W)、10的期望值(E)、M＝5、N＝-4以及两条链的比较作为默认值。对于氨基酸序列，BLASTP程序使用3的字长(W)、10的期望值(E)和BLOSUM62评分矩阵作为默认值(参见Henikoff&Henikoff,Proc.Natl.Acad.Sci.USA[美国国家科学院院刊]89:10915(1989))。
除了计算序列同一性百分比之外，BLAST算法还进行两个序列之间相似性的统计学分析(参见例如Karlin&Altschul,Proc.Nat’l.Acad.Sci.USA[美国国家科学院院刊]90:5873-5787(1993))。BLAST算法提供的相似性的一种量度是最小和概率(P(N))，其提供两个核苷酸或氨基酸序列之间偶然发生匹配的概率的指示。例如，如果在测试核酸与参考核酸的比较中最小和概率小于约0.1，更优选小于约0.01，最优选小于约0.001，则认为该核酸与参考序列相似。
两个核酸序列或多肽基本上相同的另一个指示是由第一个核酸编码的多肽与由第二个核酸编码的多肽具有免疫交叉反应性，如下所述。因此，例如，如果某多肽和第二多肽仅因保守取代而不同，则两种肽通常基本上相同。两个核酸序列基本上相同的另一个指示是两个分子在严格条件下彼此杂交，如下所述。
如本文所用，术语“保护性免疫”或“保护性免疫应答”是指该接种疫苗的受试者能够控制对其进行了疫苗接种的致病因子的感染。致病因子可以是例如抗原基因产物或抗原蛋白，或其片段。通常，已经出现了“保护性免疫应答”的受试者仅出现轻度至中度临床症状或根本没有症状。通常，对某种因子具有“保护性免疫应答”或“保护性免疫”的受试者不会由于所述因子的感染而死亡。
术语“佐剂”定义为一种或多种引起免疫系统刺激的物质。在此上下文中，使用佐剂来增强对本发明的腺病毒载体的免疫应答。
如本文所用，术语“抗原基因产物或其片段”或“抗原蛋白”可包括细菌、病毒、寄生虫、或真菌蛋白、或其片段。优选地，抗原性蛋白或抗原性基因产物能够在宿主中产生保护性免疫应答，例如诱导针对疾病或感染(例如细菌、病毒、寄生虫或真菌疾病或感染)的免疫应答，和/或在受试者中产生针对疾病或感染的免疫(即，接种疫苗)，其保护受试者抵抗疾病或感染。
腺病毒载体
暴露于某些腺病毒已经导致针对某些腺病毒血清型的免疫应答，这可以影响腺病毒载体的功效。因为人腺病毒感染在人类中是常见的，所以在人群中抗人腺病毒的中和抗体的流行率高。预计个体中存在这种中和抗体会降低基于人腺病毒骨架的基因转移载体的功效。一种避免功效降低的方法是替换腺病毒衣壳蛋白上的表位，这些表位是中和抗体的靶标。衣壳蛋白上的靶序列可以用来自其他腺病毒的蛋白序列替换，这些腺病毒具有低流行性，并因此而言，针对这些腺病毒的中和抗体在人群中很少。
“衣壳蛋白”是指腺病毒衣壳上的蛋白(例如BLY6、HAdV-4)或其功能片段或衍生物，其参与确定特定腺病毒的血清型和/或向性。衣壳蛋白典型地包括纤维蛋白、五邻体蛋白和/或六邻体蛋白。在某些实施例中，衣壳蛋白是腺病毒的完整或全长衣壳蛋白。在其他实施例中，衣壳蛋白是腺病毒的全长衣壳蛋白的片段或衍生物。在某些实施例中，由本发明的腺病毒载体编码的六邻体、五邻体和纤维具有相同或不同的腺病毒背景(即，BLY6六邻体和BLY6纤维、BLY6六邻体和人腺病毒纤维、人腺病毒六邻体和BLY6纤维等)。
“六邻体多肽”指腺病毒六邻体外壳蛋白、其功能片段和衍生物。
“纤维多肽”指腺病毒纤维蛋白、其功能片段和衍生物。
针对腺病毒的中和抗体的一个靶标是主要外壳蛋白，即六邻体蛋白。用来自人群中罕见的腺病毒的六邻体蛋白或六邻体蛋白内的可变序列，诸如本文所述的那些大猩猩序列，替换六邻体蛋白或六邻体蛋白内的定义血清型并与中和抗体结合的可变序列，可以允许构建对通常在人中发现的抗体的中和不太敏感的腺病毒载体。
针对腺病毒的中和抗体的第二个靶标是纤维蛋白。用来自人群中罕见的腺病毒的纤维蛋白或纤维蛋白内的可变序列，诸如本文所述的那些大猩猩序列，替换纤维蛋白或纤维蛋白内的可变序列，也可以允许构建对通常在人中发现的抗体的中和不太敏感的腺病毒载体。上述纤维替换与六邻体替换的组合可赋予对通常在人中发现的抗体的中和的额外抗性。
本公开提供了编码六邻体多肽和/或纤维多肽的分离的核酸序列，这些六邻体多肽和/或纤维多肽源自分离的猿猴腺病毒血清型；以及包含这些分离的核酸序列中的至少一种的腺病毒载体。
多肽的“功能衍生物”适当地是指多肽的修饰形式，例如其中可以删除、插入、修饰和/或取代多肽的一个或多个氨基酸。未修饰的腺病毒衣壳蛋白的衍生物在例如以下情况下被认为是功能性的：
(a)在其衣壳内包含衍生衣壳蛋白的腺病毒与包含未修饰的衣壳蛋白的腺病毒相比，保持基本上相同或更低的血清阳性率；和/或
(b)在其衣壳内包含衍生衣壳蛋白的腺病毒与包含未修饰的衣壳蛋白的腺病毒相比，保持基本上相同或更高的宿主细胞感染率；和/或
(c)在其衣壳内包含衍生衣壳蛋白的腺病毒与包含未修饰的衣壳蛋白的腺病毒相比，保持基本上相同或更高的免疫原性；和或
(d)在其衣壳内包含衍生衣壳蛋白的腺病毒与包含未修饰的衣壳蛋白的腺病毒相比，保持基本上相同或更高水平的转基因生产率。
“腺病毒载体”指衍生自或包含腺病毒基因组的至少一部分的重组载体。
在优选的实施例中，分离的核酸序列编码六邻体多肽或其功能衍生物，该六邻体多肽或其功能衍生物包括含六邻体高变区多肽，该含六邻体高变区多肽具有SEQ ID NO:1的氨基酸序列或在其全长上与SEQ ID NO:1的氨基酸序列具有至少95％同一性的氨基酸序列。在某些实施例中，六邻体多肽包括具有SEQ ID NO:1的氨基酸序列的含六邻体高变区多肽。在优选的实施例中，六邻体多肽或其功能衍生物包含BLY6六邻体多肽的氨基酸序列(SEQ ID NO:2)或在其全长上与SEQ ID NO:2的氨基酸序列具有至少95％同一性的氨基酸序列。在某些实施例中，六邻体多肽或其功能衍生物包含在其全长上与SEQ ID NO:2的氨基酸序列至少95％、至少96％、至少97％、至少98％或至少99％相同的氨基酸序列。
在优选的实施例中，分离的核酸序列编码纤维多肽或其功能衍生物。纤维多肽可以例如包含以下至少一者：包含SEQ ID NO:10的氨基酸序列的纤维突结多肽；包含SEQ IDNO:11的氨基酸序列的纤维轴多肽；以及包含SEQ ID NO:12的氨基酸序列的纤维尾多肽。在优选的实施例中，纤维多肽或其功能衍生物包含BLY6纤维多肽的氨基酸序列(SEQ ID NO:3)或在其全长上与SEQ ID NO:3的氨基酸序列具有至少95％同一性的氨基酸序列。
在优选的实施例中，提供了分离的核酸，其包含编码至少一种本文披露的六邻体多肽的六邻体核酸序列和编码至少一种本文披露的纤维多肽的核酸序列。
在优选的实施例中，提供了载体，优选腺病毒载体，这些载体包含根据本发明的实施例的分离的六邻体核酸序列和/或分离的纤维核酸序列中的至少一者。腺病毒载体可以例如包含至少一个转基因；以及编码六邻体多肽和/或纤维多肽的核酸序列，其中六邻体多肽包括构成本文披露的含六邻体高变区多肽的多肽，并且纤维多肽包括本文所述的纤维多肽。
典型地，本发明的腺病毒载体包含在例如质粒、粘粒或杆状病毒载体上的完整重组腺病毒基因组。本发明的核酸分子可以呈RNA形式或呈通过克隆而获得或以合成方式而产生的DNA形式。DNA可以是双链的或单链的。
普通技术人员将认识到，衍生自多种血清型的元件可以组合在单个腺病毒载体中，例如人或猿猴腺病毒。因此，可以产生组合了来自不同血清型的期望性质的嵌合腺病毒载体。因此，在一些实施例中，本发明的嵌合腺病毒载体可以将不存在预先存在的猿猴六邻体和/或纤维多肽序列免疫性与现有腺病毒载诸如rAd4、rAd5、rAd26或rAd35的高水平抗原递送和呈递能力相结合。
用作疫苗的腺病毒载体的优点包括易于操纵、良好的大规模可制造性以及基于多年的研究、开发、制造和临床试验经验的优异安全记录，这些临床试验使用了许多已报道的腺病毒载体。被用作疫苗的腺病毒载体通常提供对转基因编码的蛋白的良好免疫应答，包括细胞免疫应答。根据本发明的腺病毒载体可以基于任何类型的腺病毒，并且在某些实施例中是人腺病毒，其可以属于任何组或血清型。在优选的实施例中，重组腺病毒基于来自A、B、C、D、E、F或G组的人腺病毒。在其他优选的实施例中，重组腺病毒基于人腺病毒血清型5、11、26、34、35、48、49或50。在其他实施例中，它是可以为任何血清型的猿猴腺病毒，诸如黑猩猩或大猩猩腺病毒。在某些实施例中，重组腺病毒基于黑猩猩腺病毒1、3、7、8、21、22、23、24、25、26、27.1、28.1、29、30、31.1、32、33、34、35.1、36、37.2、39、40.1、41.1、42.1、43、44、45、46、48、49、50、67型或SA7P。
在更优选的实施例中，第二组合物的黑猩猩腺病毒载体是ChAdV3。重组黑猩猩腺病毒血清型3(ChAd3或cAd3)是亚群C腺病毒，其性质类似于人腺病毒血清型5(Ad5)的性质。ChAd3在评估丙型肝炎病毒(HCV)候选疫苗的人类研究中已显示出是安全的和免疫原性的(Barnes E等人2012Science translational medicine[科学转化医学]4:115ra 1)。据报道，基于ChAd3的疫苗能够诱导与人Ad5载体疫苗相当的免疫应答。参见例如Peruzzi D等人2009Vaccine[疫苗]27:1293-300和Quinn KM等人2013J Immunol[免疫学杂志]190:2720-35；WO 2005/071093；WO 2011/0130627等。
腺病毒载体、其构建方法及其繁殖方法是本领域所熟知的，并且描述于例如美国专利号5,559,099、5,837,511、5,846,782、5,851,806、5,994,106、5,994,128、5,965,541、5,981,225、6,040,174、6,020,191和6,113,913，以及Thomas Shenk,“Adenoviridae andtheir Replication”[腺病毒及其复制],M.S.Horwitz,“Adenoviruses”[腺病毒],分别第67和68章，见于Virology[病毒学],B.N.Fields等人编，第3版，纽约雷文出版社有限公司(Raven Press,Ltd.,New York)(1996)，以及本文提及的其他参考文献。典型地，腺病毒载体的构建涉及使用标准分子生物学技术，诸如以下文献中所述的那些技术：Sambrook等人,Molecular Cloning,a Laboratory Manual[分子克隆，实验室手册],第2版,纽约冷泉港的冷泉港出版社(Cold Spring Harbor Press,Cold Spring Harbor,N.Y.)(1989)；Watson等人,Recombinant DNA[重组DNA],第2版,Scientific American Books[科学美国人](1992)；和Ausubel等人,Current Protocols in Molecular Biology[分子生物学实验室指南],纽约威利国际科学出版公司(Wiley Interscience Publishers,NY)(1995)；以及本文提及的其他参考文献。
在某些实施例中，腺病毒载体包含E1缺失和/或E3缺失。E1或E3缺失可以例如包括基因的完全缺失或部分缺失，其使得E1或E3基因产物具有功能缺陷。因此，在某些实施例中，腺病毒是复制缺陷型的，例如因为它在基因组的E1区含有缺失。如技术人员已知的，在缺失来自腺病毒基因组的必需区域的情况下，由这些区域编码的功能必须优选的是由生产细胞反式提供，即，当E1、E2和/或E4区域的部分或全部从腺病毒中缺失时，这些区域必须存在于生产细胞中，例如整合到其基因组中，或呈所谓的辅助腺病毒或辅助质粒形式。这些腺病毒还可以具有在E3区域中的缺失，该缺失对于复制是非必要的，因此不必补充这种缺失。E1、E2、E3和E4区域中的一个或多个也可以通过其他方法失活，诸如通过将感兴趣的转基因(通常与启动子连接)插入待失活的区域。
可以使用的生产细胞(有时在本领域中以及在本文还称为‘包装细胞’或‘补充细胞’)可以是可在其中繁殖所希望的腺病毒的任何生产细胞。例如，在生产细胞中完成重组腺病毒载体的繁殖，这些生产细胞补充腺病毒中的缺陷。此类生产细胞优选地在其基因组中至少具有腺病毒E1序列，并因而能够补充在E1区域中具有缺失的重组腺病毒。可以使用任何补充E1的生产细胞，诸如由E1永生化的人类视网膜细胞，例如911或PER.C6细胞(参见美国专利5,994,128)、E1转化的羊水细胞(参见欧洲专利1230354)、E1转化的A549细胞(参见例如WO 98/39411、美国专利5,891,690)、GH329:HeLa(Gao等人,2000,Hum Gene Ther[人类基因治疗]11:213-19)、293等。在某些实施例中，生产细胞是例如HEK293细胞、或PER.C6细胞、或911细胞、或IT293SF细胞等。在(Kovesdi等人,2010,Viruses[病毒]2:1681-703)中对生产细胞中的腺病毒载体的生产进行了综述。
在某些实施例中，腺病毒载体是包含一个或多个人腺病毒核酸序列的嵌合腺病毒载体。人腺病毒核酸可以选自例如人腺病毒-4(Ad-4)、人腺病毒-5(Ad-5)、人腺病毒-26(Ad-26)或人腺病毒-35(Ad-35)。在某些实施例中，E1缺陷型腺病毒载体包含人Ad5的腺病毒的E4-orf6编码序列。这使得此类腺病毒可在表达Ad5的E1基因的熟知的补充细胞系中繁殖，诸如293细胞或PER.C6细胞(参见例如Fallaux等人,1998,Hum Gene Ther[人类基因治疗]9:1909-17；Havenga等人,2006,J Gen Virol[普通病毒学杂志]87:2135-43；WO 03/104467，它们通过援引以其全文并入本文)。
在某些实施例中，腺病毒载体包含转基因。“转基因”是指异源核酸，它是不天然存在于载体中的核酸，并且根据本发明，转基因可编码在受试者中引发免疫应答的抗原性基因产物或抗原性蛋白。转基因可以例如通过标准分子生物学技术引入载体中。例如，可将转基因克隆到腺病毒载体的缺失的E1或E3区域中，或E4区域与rITR之间的区域中。通常，转基因可操作地连接至表达控制序列。在优选的实施例中，转基因在转基因插入位点被插入。
如果需要，可以对编码根据本发明实施例的六邻体和/或纤维多肽的核酸序列和/或对转基因进行密码子优化，以确保在治疗的宿主(例如人)中正确表达。密码子优化是广泛应用于本领域的技术。
转基因可以在腺病毒衍生的启动子(例如，主要晚期启动子)的控制下(即，可操作地连接)，或者可以在异源启动子的控制下。合适的异源启动子的实例包括CMV启动子和RSV启动子。优选地，启动子位于表达盒内的感兴趣的异源基因的上游。
在优选的实施例中，腺病毒载体包含SEQ ID NO:13或SEQ ID NO:14的核酸序列。
免疫原性组合物
免疫原性组合物是用于本发明的包含免疫有效量的纯化的或部分纯化的人或猿猴(例如大猩猩)腺病毒载体的组合物。所述组合物可以根据本领域熟知的方法被配制成疫苗(也称为“免疫原性组合物”)。此类组合物可以包含佐剂以增强免疫应答。鉴于本披露，可以通过本领域技术人员熟知的技术确定制剂中每种组分的最佳比率。
鉴于本披露，根据本发明的实施例的免疫原性组合物可以使用本领域技术人员已知的方法制备。液体药物组合物通常包含液体载剂，诸如水、石油、动物或植物油、矿物油或合成油。可以包括生理盐水溶液、葡萄糖或其他糖溶液或二醇，诸如乙二醇、丙二醇或聚乙二醇。
可用于本发明的免疫原性组合物可以包含佐剂。适于根据本发明共同施用的佐剂应该是在人中潜在安全、耐受良好且有效的佐剂，包括QS-21、Detox-PC、MPL-SE、MoGM-CSF、TiterMax-G、CRL-1005、GERBU、TERamide、PSC97B、Adjumer、PG-026、GSK-I、AS01、AS03、AS04、AS15、GcMAF、B-alethine、MPC-026、Adjuvax、CpG ODN、Betafectin、Alum和MF59。
其他可以施用的佐剂包括凝集素、生长因子、细胞因子和淋巴因子，诸如α-干扰素、γ-干扰素、血小板衍生生长因子(PDGF)、粒细胞集落刺激因子(gCSF)、粒细胞巨噬细胞集落刺激因子(gMCSF)、肿瘤坏死因子(TNF)、表皮生长因子(EGF)、IL-I、IL-2、IL-4、IL-6、IL-8、IL-10和IL-12或其编码核酸。
本发明的组合物可以包含药学上可接受的赋形剂、载剂、缓冲剂、稳定剂或本领域技术人员熟知的其他材料。此类材料应该是无毒的，并且不应该干扰活性成分的功效。载剂或其他材料的确切性质可以取决于施用途径，例如肌内、皮下、经口、静脉内、皮肤、粘膜内(例如，肠)、鼻内或腹膜内途径。
诱导保护性免疫的方法
本发明的另一个一般方面涉及在需要的受试者中诱导免疫应答的方法。这些方法可以例如包括向受试者施用包含本文所述的腺病毒载体和药学上可接受的载剂的疫苗。本文还提供了生产疫苗的方法。这些方法包括将本文所述的腺病毒载体与药学上可接受的载剂组合。
根据本发明的实施例的任何免疫原性组合物(包括但不限于本文所述的那些)均可在本发明方法中用作疫苗。
包含载体的免疫原性组合物/疫苗的施用典型地是肌内或皮下。然而，也可以设想其他施用方式，诸如静脉内、皮肤、皮内或鼻。免疫原性组合物的肌内施用可以通过使用针注射腺病毒载体的混悬液来实现。一种替代方案是使用无针注射装置来施用组合物(使用例如BiojectorTM)或含有疫苗的冷冻干燥粉末。
对于静脉内、皮肤或皮下注射，或在病痛部位的注射，载体将呈肠胃外可接受的水溶液的形式，它是无热原的并具有合适的pH、等渗性和稳定性。本领域技术人员完全能够使用例如等渗媒介物诸如氯化钠注射液、林格氏注射液、乳酸盐林格氏注射液来制备适合的溶液。根据需要，可以包括防腐剂、稳定剂、缓冲剂、抗氧化剂和/或其他添加剂。还可以使用缓释制剂。
典型地，施用将具有预防性目的，以在感染或发展症状之前产生针对感兴趣的抗原(例如，细菌、病毒、寄生虫和/或真菌病原体)的免疫应答。可根据本发明治疗或预防的疾病和病症包括免疫应答可在其中起到保护或治疗作用的那些。在其他实施例中，腺病毒载体可被施用用于暴露后预防。
将含有人或猿猴(例如大猩猩)腺病毒载体的免疫原性组合物施用给受试者，从而在受试者中产生对感兴趣的抗原的免疫应答。足以诱导可检测的免疫应答的组合物的量被定义为组合物的“免疫有效剂量”或“有效量”。本发明的免疫原性组合物可以诱导体液以及细胞介导的免疫应答。在典型的实施例中，免疫应答是保护性免疫应答。
施用的实际量以及施用的速率和时程将取决于正治疗的疾病的性质和严重度。治疗处方(例如剂量等的决定)是在全科医生和其他医师、或在兽医环境中是兽医的职责范围内的，并且典型地考虑了待治疗的病症、个体患者的状况、递送部位、施用方法以及医师已知的其他因素。以上提及的这些技术和方案的实例可以在Remington's PharmaceuticalSciences[雷明顿药学大全],第16版，Osol,A.编,1980中找到。
在产生腺病毒载体和任选将此类颗粒配制成组合物后，可以将这些载体施用给个体，特别是人或其他灵长类动物。可以对人或另一种哺乳动物施用，例如小鼠、大鼠、仓鼠、豚鼠、兔、绵羊、山羊、猪、马、牛、驴、猴、狗或猫。向非人哺乳动物的递送不必是为了治疗目的，而是可以用于实验环境，例如研究对腺病毒载体的免疫应答机制。
在一个示例性方案中，腺病毒载体以约100μl至约10ml范围内的体积施用(例如肌内)，该体积的浓度为约104至1012个病毒颗粒/ml。优选地，腺病毒载体以0.1-2.0ml范围内的体积施用。例如，腺病毒载体可以100μl、500μl、1ml、2ml施用。更优选地，腺病毒载体以0.5ml的体积施用。任选地，腺病毒载体以107vp/ml、108vp/ml、109vp/ml、1010vp/ml、5x1010vp/ml、1011vp/ml或1012vp/ml的浓度施用。典型地，在一次施用期间，腺病毒载体以约109至约1012个病毒颗粒(vp)的量，更典型地以约1010至约1012个vp的量施用于人受试者。
初始疫苗接种之后可以是从包含编码感兴趣抗原的相同腺病毒载体的疫苗/组合物或包含编码相同感兴趣抗原的不同腺病毒载体的疫苗/组合物的加强或激发。
如果需要，组合物可以存在于试剂盒、包装或分配器中，该试剂盒、包装或分配器可以包含一个或多个含有活性成分的单位剂型。例如，试剂盒可以包括金属或塑料箔，诸如泡罩包装。试剂盒、包装或分配器可附有施用说明书。
本发明的组合物可以单独施用或与其他治疗联合施用(同时地或按顺序)，这取决于待治疗的病状。
实施例
本发明还提供了以下非限制性实施例。
实施例1是一种分离的核酸序列，该分离的核酸序列编码六邻体多肽或其功能衍生物，该六邻体多肽或其功能衍生物包括具有SEQ ID NO:1的氨基酸序列或在其全长上与SEQ ID NO:1的氨基酸序列具有至少95％同一性的氨基酸序列的含六邻体高变区多肽。
实施例2是一种分离的核酸序列，该分离的核酸序列编码六邻体多肽，该六邻体多肽包括具有SEQ ID NO:1的氨基酸序列的含六邻体高变区多肽。
实施例3是如实施例1-2中任一项所述的分离的核酸序列，其中该六邻体多肽或其功能衍生物包含BLY6六邻体多肽的氨基酸序列(SEQ ID NO:2)或在其全长上与SEQ ID NO:2的氨基酸序列具有至少95％同一性的氨基酸序列。
实施例4是一种分离的核酸序列，该分离的核酸序列包含如实施例1-3所述的核酸序列中的任一种，并且还包含编码纤维多肽或其功能衍生物的分离的核酸序列。
实施例5是如实施例4所述的分离的核酸序列，其中该纤维多肽包含纤维突结多肽序列，该纤维突结多肽序列包含SEQ ID NO:10的氨基酸序列。
实施例6是如实施例4所述的分离的核酸序列，其中该纤维多肽包含纤维轴多肽序列，该纤维轴多肽序列包含SEQ ID NO:11的氨基酸序列。
实施例7是如实施例4所述的分离的核酸序列，其中该纤维多肽包含纤维尾多肽序列，该纤维尾多肽序列包含SEQ ID NO:12的氨基酸序列。
实施例8是如实施例4-7中任一项所述的分离的核酸序列，其中该纤维多肽或其功能衍生物包含BLY6纤维多肽的氨基酸序列(SEQ ID NO:3)或在其全长上与SEQ ID NO:3的氨基酸序列具有至少95％同一性的氨基酸序列。
实施例9是一种分离的核酸序列，该分离的核酸序列编码包含纤维突结多肽序列的纤维多肽，其中该纤维突结多肽序列包含SEQ ID NO:10的氨基酸序列。
实施例10是一种分离的核酸序列，该分离的核酸序列编码包含纤维轴多肽序列的纤维多肽，其中该纤维轴多肽序列包含SEQ ID NO:11的氨基酸序列。
实施例11是一种分离的核酸序列，该分离的核酸序列编码包含纤维尾多肽序列的纤维多肽，其中该纤维尾多肽序列包含SEQ ID NO:12的氨基酸序列。
实施例12是如实施例9-11中任一项所述的分离的核酸序列，其中该纤维多肽包含BLY6纤维多肽的氨基酸序列(SEQ ID NO:3)。
实施例13是一种载体，该载体包含如实施例1-12中任一项所述的核酸。
实施例14是如实施例13所述的载体，该载体是腺病毒载体，并且进一步包含转基因。
实施例15是一种重组细胞，该重组细胞包含如实施例13或14所述的载体。
实施例16是一种生产载体的方法，该方法包括：(a)使如实施例15所述的重组细胞在用于生产该载体的条件下生长；以及(b)从该重组细胞中分离该载体。
实施例17是一种免疫原性组合物，该免疫原性组合物包含如实施例14所述的载体。
实施例18是一种在有需要的受试者中诱导免疫应答的方法，该方法包括向该受试者施用如实施例17所述的免疫原性组合物。
实施例19是一种腺病毒载体，该腺病毒载体包含(a)至少一个转基因；以及(b)编码六邻体多肽的核酸序列，该六邻体多肽包括具有SEQ ID NO:1的氨基酸序列的含六邻体高变区多肽。
实施例20是一种腺病毒载体，该腺病毒载体包含(a)至少一个转基因；以及(b)编码六邻体多肽的核酸序列，该六邻体多肽包含BLY6六邻体多肽的氨基酸序列(SEQ ID NO:2)。
实施例21是一种腺病毒载体，该腺病毒载体包含(a)至少一个转基因；编码六邻体多肽的核酸序列，该六邻体多肽包含BLY6六邻体多肽的氨基酸序列(SEQ ID NO:2)；以及(c)编码纤维多肽的核酸序列，该纤维多肽包含BLY6纤维多肽的氨基酸序列(SEQ ID NO:3)。
实例
实例1：基于新型腺病毒分离株BLY6的E1和E3缺失型载体的生成
鉴定了新型大猩猩腺病毒分离株BLY6(也称为JAd1-WT)并测序。发现该大猩猩腺病毒分离株在系统发育上属于人腺病毒E种(HAdV-E)。确定BLY6的全基因组核苷酸序列为SEQ ID NO:5。
用于生成基于BLY6的腺病毒载体的单质粒系统的描述
pBLY6.dE1.dE3(SEQ ID NO:8；图5)和pBLY6.dE1.dE3.5IXP(SEQ ID NO:9；图6)是携带基于分离株BLY6的全长、E1和E3缺失型腺病毒载体基因组的质粒。这些质粒中包含的腺病毒载体基因组序列分别在SEQ ID NO:13和SEQ ID NO:14中列出。在这些质粒的每一个中，腺病毒载体基因组的侧翼是两个SwaI限制性酶切位点(即一个SwaI位点位于载体基因组的任一端)。这些SwaI位点是为了在病毒拯救之前通过转染合适的E1补充细胞(例如HEK293、911和PER.C6细胞)来促进从质粒骨架上切除腺病毒载体基因组。这些质粒所包含的腺病毒载体基因组进一步携带某些限制性酶切位点，这些限制性酶切位点在E1缺失的位置中、在E3缺失中以及在右反向末端重复序列(RITR)附近被引入。在完整的腺病毒基因组质粒的情况下，将这些限制性酶切位点选择为独特的。它们代表“转基因插入位点”，这些位点允许通过标准分子克隆技术容易地构建携带一个或多个转基因表达盒的腺病毒载体，该一个或多个转基因表达盒插入在所述相应位置中的任一个处或其任何组合中。腺病毒载体设计和质粒构建在以下章节中更详细地描述。
基于BLY6的腺病毒载体基因组设计
基于BLY6的腺病毒载体基因组分别设计为包含E1缺失、E3缺失、不同的转基因插入位点以及天然E4开放阅读框(orf)6和orf6/7被人腺病毒-5(HAdV-5)的开放阅读框替换。使每种腺病毒的E1区域缺失，并用包含AsiSI限制性酶切位点序列的转基因插入位点替换。使每种腺病毒的E3区域缺失，并用包含FseI限制性酶切位点序列的转基因插入位点替换。通过在每个腺病毒的右反向末端重复序列(ITR)附近插入PacI限制性酶切位点序列，产生另一个转基因插入位点。包含E4 orf6和orf6/7编码序列的BLY6序列被SEQ ID NO:6替换。该替换序列包含人腺病毒-5(HAdV-5)的E4 orf6和orf6/7编码序列(GenBank序列AC_000008的碱基对32914-34077)。
设计和构建了两种类型的E1区域缺失。pBLY6.dE1.dE3所包含的基于BLY6的腺病毒载体基因组携带对应于SEQ ID NO:5的核苷酸453至3016的移除的E1区域缺失。相比之下，pBLY6.dE1.dE3.5IXP所包含的基于BLY6的腺病毒载体基因组携带较大的包含E1区域的序列缺失，从而移除了BLY6的所有E1编码序列(即，SEQ ID NO:5的核苷酸453至3366)。此外，该后一个腺病毒载体基因组还被设计成携带E1B 55K与pIX编码序列之间的非编码序列段被HAdV-5的非编码序列段替换(即，对应于SEQ ID NO:5的核苷酸3367至3454的序列被GenBank AC_000008的核苷酸3510-3608替换(即，被SEQ ID NO:7替换))。
包含基于BLY6的腺病毒载体基因组的单质粒的构建
通过若干基因合成步骤(由金斯瑞公司执行)和标准分子克隆程序构建了pBLY6.dE1.dE3(SEQ ID NO:8)。首先，合成含有所需腺病毒载体基因组的左端(即带有上述E1缺失)的3586bp DNA片段(SEQ ID NO:15)，并作为MfeI-NdeI限制性片段连接到EcoRI-和NdeI消化的pBR322(GenBank登录号-J01749.1)中，从而产生BLY6中间质粒1。其次，合成含有所需腺病毒载体基因组的右端(即带有上述E3缺失、部分E4序列替换和rITR附近的转基因插入位点)的4138bp片段(SEQ ID NO:16)，并作为BamHI-NdeI限制性片段连接到BamHI和NdeI消化的BLY6中间质粒1中，从而产生BLY6中间质粒2。第三，合成含有中间腺病毒载体基因组片段的4563bp片段(SEQ ID NO:17)，并作为BamHI-MfeI限制性片段连接到BamHI和MfeI消化的BLY6中间质粒2中，从而产生BLY6中间质粒3(SEQ ID NO:18)。第四，将BLY6病毒基因组的18987bp BsrGI-BsrGI限制性片段(SEQ ID NO:5)连接到BsrGI消化的BLY6中间质粒3中，从而产生最终的质粒pBLY6.dE1.dE3(SEQ ID NO:8)。
以与pBLY6.dE1.dE3相同的方式构建pBLY6.dE1.dE3.5IXP(SEQ ID NO:9)，不同的是，首先修饰上述BLY6中间质粒3(SEQ ID NO:18)以包含所需的E1缺失和Ad5 pIX启动子插入。这是通过合成638bp片段(SEQ ID NO:19)完成的，该片段随后作为AsiSI-AgeI限制性片段连接到AsiSI和AgeI消化的BLY6中间质粒3中。
pBLY6.FLuc(SEQ ID NO:20)和pBLY6.RSVF-2A-GLuc(SEQ ID NO:21)是pBLY6.dE1.dE3衍生的质粒，它们各自带有基于BLY6的腺病毒载体基因组，该腺病毒载体基因组装备有在E1缺失位置插入的转基因表达盒。这些质粒内携带的腺病毒载体基因组序列分别在SEQ ID NO:22和SEQ ID NO:23中列出。pBLY6.FLuc携带萤火虫萤光素酶(FLuc)的转基因表达盒。该盒由巨细胞病毒主要立即早期启动子(即“CMV启动子”)驱动，并含有SV40衍生的聚腺苷酸化信号。pBLY6.RSVF-2A-Gluc携带“RSV-FA2-2A-GLuc”的转基因表达盒(RSVF-2A-GLuc)，它是由呼吸道合胞病毒株A2融合糖蛋白、口蹄疫病毒2A肽和高斯(Gaussia)萤光素酶(GLuc)构成的嵌合蛋白。像Fluc盒一样，该盒也由CMV启动子驱动，并携带SV40聚腺苷酸化信号。另外，该盒在其5’非翻译区中包含含有人载脂蛋白A1基因的内含子2的序列。将Fluc和RSVF-2A-GLuc表达盒各自通过若干标准基因合成和分子克隆步骤构建，然后将它们连接到pBLY6.dE1.dE3的独特AsiSI限制性酶切位点中，从而分别生成pBLY6.FLuc和pBLY6.RSVF-2A-Gluc。
基于BLY6的腺病毒载体的生成和生产
通过将相应的腺病毒载体基因组质粒(即，pBLY6.Fluc和pBLY6.RSVF-2A-Gluc)转染到补充E1的PER.C6细胞中，生成了分别包含腺病毒载体基因组序列SEQ ID NO:22和SEQID NO:23的腺病毒载体BLY6.Fluc(也称为JAd1NVT003)和BLY6.RSVF-2A-Gluc(也称为JAd1NVT001)。在转染到PER.C6细胞(这些细胞在补充了10％胎牛血清(FBS)和10mM MgCl2的达尔伯克氏改良伊格尔培养基(DMEM)中作为贴壁培养物生长)中之前，用SwaI消化腺病毒载体基因组质粒以从质粒释放相应的腺病毒载体基因组。使用Lipofectamine转染试剂(加利福利亚州卡尔斯巴德的英杰公司(Invitrogen；Carlsbad,CA))，根据标准程序进行转染。在收获病毒拯救转染后，通过在PER.C6细胞培养物上的连续几轮感染而进一步扩增病毒。如前所述(Havenga等人,“Novel replication-incompetent adenoviral B-groupvectors:high vector stability and yield in PER.C6 cells[新的无复制能力的腺病毒B组载体：在PER.C6细胞中的高载体稳定性和产率]”J.Gen.Virol.[普通病毒学杂志]87(8):2135-43(2006))，使用两步氯化铯(CsCl)密度梯度超速离心程序从粗病毒收获物中纯化病毒。病毒颗粒(VP)滴度通过先前描述的基于分光光度法的程序进行测量(Maizel等人,“The polypeptides of adenovirus:I.Evidence for multiple protein components inthe virion and a comparison of types 2,7A,and 12,”[腺病毒的多肽：I.病毒体中多种蛋白组分的证据以及2、7A和12型的比较]Virology[病毒学],36(1):115-25(1968))。
由新型腺病毒载体诱导的细胞和体液免疫应答
实例2和3描述了为了评估本文生成的新型基于BLY6的腺病毒载体的免疫原性而进行的实验。在这些实验中，评估了新型载体在小鼠中肌内免疫后诱导针对载体编码的(模型)抗原的体液和细胞免疫应答的能力。使用两种不同的抗原测试了载体：萤火虫萤光素酶(FLuc)和RSV-FA2-2A-GLuc(RSVF-2A-GLuc)。RSVF-2A-GLuc是一种嵌合蛋白，由呼吸道合胞病毒株A2融合糖蛋白、口蹄疫病毒2A肽和高斯萤光素酶(GLuc)组成。将每个载体与基于携带相同的编码抗原的转基因盒的人26型腺病毒(HAdV-26，在本文中也称为Ad26)或人49型腺病毒(HAdV-49，在本文也称为Ad49)的基准载体并排比较。使用众所周知的免疫测定法，诸如酶联免疫斑点测定法(ELISPOT)、酶联免疫吸附测定法(ELISA)以及在RSVF-2A-GLuc抗原的情况下，呼吸道合胞病毒中和测定法(VNA)，测量了针对相应抗原的免疫应答。
实例2：BLY6.FLuc诱导的细胞免疫应答
为了评估新型腺病毒载体BLY6的细胞免疫原性，通过肌内注射Ad26.FLuc、Ad49.Fluc(阳性对照)、表达萤火虫萤光素酶的BLY6载体(BLY6.FLuc)或不编码转基因的腺病毒载体(Ad26空)免疫Balb/C小鼠。测试了两个载体施用剂量：每只小鼠109和1010个病毒颗粒(vp)。免疫两周后，处死小鼠，并用15mer重叠的FLuc肽库刺激脾细胞过夜(实验设置图1A)。通过离体ELISPOT试验测定了细胞免疫应答，该试验测量IFN-γ分泌细胞的相对数量(图1B)。结果表明，在较高剂量免疫(1010)下，BLY6诱导的细胞免疫应答大约与Ad26.Fluc所见的应答一样高。相比之下，在低剂量免疫(109)下，BLY6.Fluc比Ad26.Fluc产生了更强的应答。
总的来说，由本发明的表达FLuc的重组BLY6腺病毒载体诱导的细胞免疫应答清楚地表明了该载体在小鼠中的强有力的免疫原性。
实例3：由BLY6.RSVF-2A-GLuc诱导的细胞和体液免疫应答
使用RSV-FA2-2A-GLuc(RSVF-2A-GLuc)作为载体编码的(模型)疫苗抗原，进一步评估了新型腺病毒载体BLY6的免疫原性。用Ad26.RSVF-2A-GLuc(阳性对照)或BLY6.RSVF-2A-GLuc(均以每只小鼠108、109和1010个病毒颗粒)或者用Ad26.FLuc或BLY6.FLuc(均以每只小鼠1010个病毒颗粒)肌内免疫Balb/C小鼠。在八周后处死小鼠并收集血样和脾细胞(图2A)。如下所述评估不同的免疫参数。
为了评估BLY6.RSVF-2A-GLuc引发呼吸道合胞病毒中和抗体的能力，进行了病毒中和试验。图2B描绘了免疫后八周时收集的血清样品所测得的呼吸道合胞病毒株A2(RSVA2)VNA滴度。每个点代表一只小鼠；条代表组平均值，虚线对应于定量下限(LLOQ＝6,88；线性样品的平均终点滴度)。结果表明，用BLY6.RSVF-2A-GLuc以1010vp剂量免疫所产生的RSVA2中和滴度比编码相同抗原的基准Ad26载体所见的中和滴度更高。主要以用于免疫的最高剂量1010vp检测针对BLY6.RSVF-2A-GLuc的滴度。正如预期的那样，未检测到针对编码萤火虫萤光素酶的腺病毒载体的RSV A2特异性应答。
通过RSV-FA2特异性ELISPOT试验评估了针对载体编码的抗原的细胞免疫诱导。为此，在免疫后八周时，分离来自免疫小鼠的脾细胞，用跨越RSV-FA2蛋白的15mer重叠肽刺激过夜，通过离体ELISPOT试验测定细胞免疫应答，该试验测量IFN-γ分泌细胞的相对数量。数据显示，由编码RSVF-2A-GLuc的新型载体BLY6引发的抗原特异性细胞免疫应答是剂量依赖性的，并且根据剂量，在大小上与基准载体Ad26.RSVF-2A-GLuc诱导的免疫应答相似(图2C)。正如预期的那样，从用编码萤火虫萤光素酶的腺病毒载体免疫的小鼠的脾细胞中没有测量到RSVF-FA2特异性应答。
通过ELISA评估了表达RSVF-2A-GLuc的载体引发RSV-FA2特异性IgG抗体的能力。在抗RSV FA2 IgG抗体ELISA中测试了从用表达RSVF-2A-GLuc转基因或萤火虫萤光素酶(对照)的Ad26(阳性对照)和BLY6载体免疫的小鼠免疫后8周时收集的血清。具体而言，此ELISA检测能够结合重组稳定的融合前RSV-FA2蛋白(前RSV-F)的IgG抗体。结果表明，BLY6.RSVF-2A-GLuc以剂量依赖性方式引发了比Ad26.RSVF-2A-GLuc所诱导的更高的前RSV-F特异性IgG抗体滴度(图2D)。正如预期的那样，在仅用编码萤火虫萤光素酶的载体免疫的小鼠的血清中未检测到RSV-FA2特异性抗体滴度。
总体而言，数据显示，BLY6载体诱导了针对所编码的抗原的强有力的细胞和体液免疫应答，与基于HAdV-26的基准载体所诱导的相似或更高。这些免疫应答清楚地表明了BLY6载体在小鼠中的强有力的免疫原性。
实例4：对新型和现有腺病毒载体之间的血清交叉中和的评估
由于它们作为新腺病毒疫苗载体的潜在用途，本文产生的新型BLY6腺病毒载体优选地在血清学上不同于目前已经开发作为疫苗载体(诸如基于人腺病毒血清型HAdV-5和HAdV-35的载体)的现有腺病毒载体。因此，在新型BLY6腺病毒载体以及基于HAdV-4、HAdV-5、HAdV-26、HAdV-35和HAdV49的几种现有载体之间进行了交叉中和测试。为此，在腺病毒中和试验中，针对每种不同的载体测试小鼠抗血清，每种抗血清针对这些腺病毒载体之一而产生。在将Balb/C小鼠用每只小鼠1010个载体颗粒免疫后两周或八周时，从这些小鼠收集用于该试验的小鼠抗血清。如先前所述(Spangers等人2003.J.Clin.Microbiol.[临床微生物学杂志]41:5046-5052)进行腺病毒中和试验。简而言之，从1:16稀释开始，将血清进行2倍系列稀释，然后与表达萤火虫萤光素酶(FLuc)的腺病毒载体预混合，然后与A549细胞温育过夜(以每个细胞500个病毒颗粒的感染复数)。感染后24小时测得的被感染细胞裂解物中的萤光素酶活性水平代表载体感染效率。针对给定载体的中和滴度定义为能够使载体感染效率降低90％的最高血清稀释度。中和滴度可任意分为以下几类：<16(无中和)、16至200、200至2,000和>2,000。结果表明，在所测试的载体之间没有大的交叉中和(图3)。在载体BLY6与Ad26之间仅观察到一些轻微的单向交叉中和，其中BLY6抗血清显示出针对Ad26的16.12的中和滴度(即，刚好高于16的检测下限)，而Ad26抗血清未显示出针对BLY6的中和滴度。因此，新的腺病毒载体BLY6未显示出或仅显示出非常轻微的与测试组中包括的人腺病毒载体即Ad26、Ad35、Ad49、Ad5和Ad4的交叉中和。因此，该载体可以潜在地与一种或多种这些或其他不同的腺病毒载体联合用于顺序免疫，例如在异源初免-加强疫苗接种方案的情况下，或者，可替代地或另外地，在一系列针对不同疾病或抗原的两个或更多个连续疫苗接种方案的情况下。
实例5：新型腺病毒载体在人群中的血清阳性率
对于它们作为有效疫苗载体的潜在用途而言重要的是，本文所述的新型腺病毒载体不受疫苗目标群体中高水平的预先存在的抗载体体液免疫的妨碍。因此，在200个来自成年人的人群血清样品中评价了BLY6载体的血清阳性率，这些成年人年龄为18-55岁，生活在美国(US)和欧盟(EU)。通过进行如实例3中所开展的且如先前所述的(Spangers等人2003.J.Clin.Microbiol.[临床微生物学杂志]41:5046-5052)标准腺病毒中和试验，测试了载体被人血清样品中和的情况。简而言之，从1:16稀释开始，将血清进行2倍系列稀释，然后与表达萤火虫萤光素酶(FLuc)的腺病毒载体预混合，然后与A549细胞温育过夜(以每个细胞500个病毒颗粒的感染复数)。感染后24小时测得的被感染细胞裂解物中的萤光素酶活性水平代表载体感染效率。针对给定载体的中和滴度定义为能够使载体感染效率降低90％的最高血清稀释度。中和滴度可任意分为以下几类：<16(无中和)、16至300、300至1,000、1000至4000和>4000。
结果表明，在所研究的人类受试者中，BLY6腺病毒载体的血清阳性率明显低于对照Ad5载体以及基准Ad26和Ad35载体(图4)。此外，针对新型BLY6载体所见的阳性中和滴度通常相当低，大部分不高于300。相比之下，大多数针对Ad26和Ad5发现的阳性中和滴度高于300。
总体而言，上述数据表明，可以认为在所评估的疫苗目标群体中，针对BLY6载体的预先存在的体液抗载体免疫是较低的，这表明这些载体在这些群体中具有作为有效疫苗载体的潜力。
实例6：在悬浮PER.C6细胞中的腺病毒载体生产率
用于临床试验及其他方面的腺病毒载体需要在可扩展的无血清腺病毒生产平台中容易地生产至高滴度。悬浮-适应的
Figure BDA0002475400260000271
细胞(本文也称为悬浮PER.C6细胞或sPER.C6)代表了这样的平台，因为它们已经显示出支持在生物反应器中大规模生产腺病毒载体，获得大量高滴度的临床级载体制备物，例如基于HAdV-26或HAdV-35的E1缺失型载体的制备物(EP 2536829B1、EP 2350268B1)。
作为关于本文所述的新型载体是否适合基于sPER.C6细胞的生产工艺的初步评估，对在摇瓶中培养的sPER.C6细胞进行了小规模载体生产率实验。这些生产率实验使用实例1中所述的新型腺病毒载体BLY6的Fluc编码形式进行。基于HAdV-26的载体Ad26.Fluc用作基准对照。将以1×106个细胞/ml的密度接种在摇瓶中的总体积为10ml的补充了4mM L-谷氨酰胺(龙沙集团(Lonza))的
Figure BDA0002475400260000272
培养基(可从龙沙集团获得)中的悬浮PER.C6细胞培养物用不同的载体以不同的病毒颗粒(VP)-细胞比感染，然后温育4天。用于感染的不同VP-细胞比是70、150和900。每天采集被感染的细胞培养物的样品，并通过基于定量PCR(qPCR)的方案测定这些样品中的VP滴度，该方案采用对CMV启动子(其存在于所有测试的载体中)特异的引物和探针。该方案需要在qPCR之前对测试样品进行DNA酶处理，以除去任何游离的载体DNA(即未包装到病毒颗粒中的载体基因组)。
新型载体BLY6.Fluc所得的生产率结果如图8所示。在所测试的所有VP-细胞感染比和收获时间点下，BLY6.Fluc显示出比基准对照载体Ad26.Fluc更高的VP滴度。这些结果证明了新型BLY6载体在基于sPER.C6的无血清悬浮细胞培养模型上的良好生产率。
总之，如上所述，对由本发明的新型重组基于BLY6的腺病毒载体诱导的体液和细胞免疫应答进行的研究清楚地表明这些载体在小鼠中的强有力的免疫原性。另外，这些载体证实不诱导或仅诱导非常有限的针对某些现有腺病毒疫苗载体候选物(例如Ad26和Ad35)的交叉中和抗体应答，反之亦然。此外，这些新载体在人类中显示出低血清阳性率。最后，这些新载体可以容易地以高产率产生。低血清阳性率、强有力的免疫原性和可生产性的组合表明本发明的新型腺病毒载体可用作针对多种病原体的新型疫苗载体候选物，并且可另外用于基因疗法和/或诊断。
本领域技术人员将理解，在不偏离本发明的广泛发明构思的情况下，可以对上述实施例进行改变。因此，应当理解，本发明不限于所披露的特定实施例，而是旨在涵盖由本说明书限定的本发明的精神和范围内的修改。
序列表
<110> 扬森疫苗与预防公司
<120> 腺病毒及其用途
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Asp Ser Ser Glu Ala Asn Leu Thr Gln Gln Ala Met Ala Asn Arg Pro
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Asn Tyr Ile Gly Phe Arg Asp Asn Phe Ile Gly Val Met Tyr Tyr Asn
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Leu Leu Asp Ser Leu Gly Asp Arg Thr Arg Tyr Phe Ser Met Trp Asn
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Met Ala Thr Pro Ser Met Leu Pro Gln Trp Ala Tyr Met His Ile Ala
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Gly Gln Asp Ala Ser Glu Tyr Leu Ser Pro Gly Leu Val Gln Phe Ala
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Arg Ala Thr Asp Thr Tyr Phe Asn Leu Gly Asn Lys Phe Arg Asn Pro
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Thr Gln Gln Ala Met Ala Asn Arg Pro Asn Tyr Ile Gly Phe Arg Asp
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Asn Phe Ile Gly Val Met Tyr Tyr Asn Ser Thr Gly Asn Met Gly Val
325 330 335
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Arg Thr Arg Tyr Phe Ser Met Trp Asn Gln Ala Val Asp Ser Tyr Asp
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Asp Lys Asp Asn Asp Lys Leu Thr Leu Trp Thr Thr Ala Asp Pro Ser
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Leu Val Ser Leu Lys Phe Asn Asn Asn Gly Val Leu Leu Ser Asn Ser
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Met Ala Thr Pro Ser Met Leu Pro Gln Trp Ala Tyr Met His Ile Ala
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Pro Thr Asn Val Lys Gly Gly Gln Ala Lys Val Lys Thr Glu Glu Asn
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Val Gln Ser Phe Asp Ile Asp Leu Ala Phe Phe Asp Ile Pro Ser Thr
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Gly Thr Gly Ser Asn Gly Thr Asn Val Asn Asp Lys Pro Asp Met Val
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Tyr Lys Pro Gly Thr Ser Asp Asp Ser Ser Glu Ala Asn Leu Cys Gln
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Ile Gly Leu Met Tyr Tyr Asn Ser Thr Gly Asn Met Gly Val Leu Ala
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Gly Gln Ala Ser Gln Leu Asn Ala Val Val Asp Leu Gln Asp Arg Asn
340 345 350
Thr Glu Leu Ser Tyr Gln Leu Leu Leu Asp Ser Leu Gly Asp Arg Thr
355 360 365
Arg Tyr Phe Ser Met Trp Asn Gln Ala Val Asp Ser Tyr Asp Pro Asp
370 375 380
Val Arg Ile Ile Glu Asn His Gly Val Glu Asp Glu Leu Pro Asn Tyr
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Ala Ser Gln Asn Gln Ile Cys Lys Gly Asn Ile Tyr Ala Met Glu Ile
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Leu Tyr Leu Pro Asp Ser Tyr Lys Tyr Thr Pro Ala Asn Ile Thr Leu
465 470 475 480
Pro Thr Asn Thr Asn Thr Tyr Asp Tyr Met Asn Gly Arg Val Val Pro
485 490 495
Pro Ser Leu Val Asp Ala Tyr Ile Asn Ile Gly Ala Arg Trp Ser Leu
500 505 510
Asp Pro Met Asp Asn Val Asn Pro Phe Asn His His Arg Asn Ala Gly
515 520 525
Leu Arg Tyr Arg Ser Met Leu Leu Gly Asn Gly Arg Tyr Val Pro Phe
530 535 540
His Ile Gln Val Pro Gln Lys Phe Phe Ala Ile Lys Ser Leu Leu Leu
545 550 555 560
Leu Pro Gly Ser Tyr Thr Tyr Glu Trp Asn Phe Arg Lys Asp Val Asn
565 570 575
Met Ile Leu Gln Ser Ser Leu Gly Asn Asp Leu Arg Thr Asp Gly Ala
580 585 590
Ser Ile Ser Phe Thr Ser Ile Asn Leu Tyr Ala Thr Phe Phe Pro Met
595 600 605
Ala His Asn Thr Ala Ser Thr Leu Glu Ala Met Leu Arg Asn Asp Thr
610 615 620
Asn Asp Gln Ser Phe Asn Asp Tyr Leu Ser Ala Ala Asn Met Leu Tyr
625 630 635 640
Pro Ile Pro Ala Asn Ala Thr Asn Val Pro Ile Ser Ile Pro Ser Arg
645 650 655
Asn Trp Ala Ala Phe Arg Gly Trp Ser Phe Thr Arg Leu Lys Thr Lys
660 665 670
Glu Thr Pro Ser Leu Gly Ser Gly Phe Asp Pro Tyr Phe Val Tyr Ser
675 680 685
Gly Ser Ile Pro Tyr Leu Asp Gly Thr Phe Tyr Leu Asn His Thr Phe
690 695 700
Lys Lys Val Ser Ile Thr Phe Asp Ser Ser Val Ser Trp Pro Gly Asn
705 710 715 720
Asp Arg Leu Leu Thr Pro Asn Glu Phe Glu Ile Lys Arg Thr Val Asp
725 730 735
Gly Glu Gly Tyr Asn Val Ala Gln Cys Asn Met Thr Lys Asp Trp Phe
740 745 750
Leu Val Gln Met Leu Ala His Tyr Asn Ile Gly Tyr Gln Gly Phe Tyr
755 760 765
Val Pro Glu Gly Tyr Lys Asp Arg Met Tyr Ser Phe Phe Arg Asn Phe
770 775 780
Gln Pro Met Ser Arg Gln Val Val Asp Glu Val Asn Tyr Lys Asp Tyr
785 790 795 800
Gln Ala Val Thr Leu Ala Tyr Gln His Asn Asn Ser Gly Phe Val Gly
805 810 815
Tyr Leu Ala Pro Thr Met Arg Gln Gly Gln Pro Tyr Pro Ala Asn Tyr
820 825 830
Pro Tyr Pro Leu Ile Gly Lys Ser Ala Val Thr Ser Val Thr Gln Lys
835 840 845
Lys Phe Leu Cys Asp Arg Val Met Trp Arg Ile Pro Phe Ser Ser Asn
850 855 860
Phe Met Ser Met Gly Ala Leu Thr Asp Leu Gly Gln Asn Met Leu Tyr
865 870 875 880
Ala Asn Ser Ala His Ala Leu Asp Met Asn Phe Glu Val Asp Pro Met
885 890 895
Asp Glu Ser Thr Leu Leu Tyr Val Val Phe Glu Val Phe Asp Val Val
900 905 910
Arg Val His Gln Pro His Arg Gly Val Ile Glu Ala Val Tyr Leu Arg
915 920 925
Thr Pro Phe Ser Ala Gly Asn Ala Thr Thr
930 935
<210> 5
<211> 35873
<212> DNA
<213> 人工序列
<220>
<223> BLY6基因组
<400> 5
catcatcaat aatatacctt atatgggcag tgtgccaata tgcaaattag acgagtttga 60
attttgcggg atgtgcggtg attggcccgg ggtcaacggt cgcggggcgg ggcggcgtga 120
cgcggacgat gacgttttgg ggaggaggag ctatgttgca agtaatcgtg ggaaatgcga 180
cgtaaaacga ggtggagttt aaacacggaa gtagacaatt ttcccgcgct gtttgacagg 240
aaatgatgtg tttttgggcg gatgcaagtg aaaattctcc attttcgcgc gaaaactaaa 300
tgaggaagtg aaattctgag taattctgag gttatcacag ggcggagtat ttaccgaggg 360
ccgagtagac tttgacccat tacgtggagg tttcgattac tctatttttc acctaaattt 420
ccgcgtactg tgtcaaagtc cgtgttttta cgtaactgtc agctgatccc tagtgtattt 480
aaacctgtgc agttccgtca agaggccact cttgagtgcc agcgagaaga gttttctcct 540
ccgcagcgcg agtcaaatct acacttctaa gatgagacac cgagtgattc ctgaggagaa 600
attctctatt agcgccggac tggaaattct acagatggtg gtggatattc taacagaggg 660
agaaccggag gctgctaact ttaactacga tcctccttcg ctccacgatc tgtatgattt 720
agaggtaaat gaaccagaga ctgaggcatt tgtgaatgcc gcgtttcctg attctctcct 780
gagtaaagct gaggagagtg agggttccag cgagcctgca actccaagtc ctccgagacc 840
tggaataggc gaaaaaagat tgcctggatg tcataaatcg cctgttgggg aaatggactt 900
gatgtgttat gaggacggct ttcctccgag cgatgaggag gaagatacac agcaggtgct 960
tgaagcctcc actagtcatg gaacggaggt gtttaatctc gactgcccag agctgcccgg 1020
ccatggctgt aagtcttgtg attatcatcg aaagagcact ggcttggagg gattgttatg 1080
ctcgctttgc tatatgcgcc taaacagcca ttatattttc agtaagtttt tttaattttg 1140
gtttaaaggg acagcgcagc gtgtctgatt gtattaagat ttggaaactt aatgtttgtg 1200
tatgtgtttc ttatataggc cctgtgtctg actcagatga gacccccacc ccagactcca 1260
ctacctcacc cccggaaatt cagatgcata ttccagagaa tgtgtgtaag ccaattcctt 1320
tgagacccac gcctggaaaa cgtcatgctg ttgaaagact tgaggactta ttgatgggtg 1380
gggatgaacc tttggacttg agcttgccta aacagccaag gcaataaaat gtccacacct 1440
gtgtttgctc aatgatgtca tgcaataaat tatgtcacat accaaaggtg tgttttatga 1500
ctaggggtgg ggctactggg tatataagta ggagcagact gttgtagtta gctgagagca 1560
gcgagctgtc tctatggagg tttgggctgc attggaagat ctccataaca ctagatccct 1620
gctagaaaac gcctcggacg gtgtctctag aatttggaga tactggtttg gtggcgagct 1680
agctagtgtg atctataggg ttaagcagga atataagggc gaatttgaag aacttttact 1740
ggactgtcct ggtcttttgg agtctttgaa tttaggtcat cagatccagt ttaacgagaa 1800
gattttatca gctttagatt tttcaacacc aggcagatct gcagcttctg ttgcttttat 1860
agttcacatt ttagacagat ggagtcagga aactcaccta agttgggggt acctcttgga 1920
tttcttagcg acagcgttgt ggagacaatg gaagagggtc agggcgagag taacctcaga 1980
ttattggccg gtgcagccgc tgggtgtagc acatctgctg agacacacca tggaactacc 2040
aacaattccg gaggaaccag ccctggagga gcaggaagag aacccgagag ccggcctgga 2100
ccctccaacg gaggaggagg aataagtgac ttgttccctg aactgcgacg ggtgcttact 2160
aggagtacat ctggaagggg ggataggggc gtgaagcgag aaaggaagga gtctaatcct 2220
aaagtggatc ttacagttag cttgatgaat cgcaaccgtc cagaggttat tacatggact 2280
gaggtgcaac aggaatgcag ggatgaattt tcccttttac aggagaaata ctcattagaa 2340
caggtgaaga catattggct ggagccagaa gatgattggg aggcagtgat tagaaattat 2400
gctaaggtgg ctcttagacc agataaaaag tatatagtaa ctaaaaagat ctgtgttagg 2460
aatgcttgct acattgtggg taatggggca gaggttcaga taaacaccac agatagggtg 2520
gcttttagat gttgtatgat gggcatgaga cctggggtga gtgggatgga ggctgtgaca 2580
tttatgaata taaaattcag aggagatggg tttaatggca cagtgtttat ggccaatact 2640
aaattaattc tgcatgggtg cagtatgttt gggtttaata atacttgtgt ggaagcctgg 2700
ggggaggtga gcataagggg gtgcagtatc tttggttgtt ggacgggagt ggttggtagg 2760
acaaagagca tggtatctgt gaagaaatgt atgtttgaaa ggtgcacact gggtatcctg 2820
agcgagggag aagctaggat tcgcaactgt gctttcacag atagtgggtg tggcatactt 2880
gtgaagggaa atgccagtat aaagcataat atgttctgcg gaccatccga tgatcgatca 2940
taccagatgg tgacctgtgc cagtggcaat tgcgatttgc tggctactat tcacattgtg 3000
acccatcaac gcaaaagatg gcctgtgttt gagcataatg tactgaccag gtgtaacgtt 3060
catctgggtg gtcgtagagg aatgttcatg ccataccaat gcaattttaa tcatgtgagg 3120
atcttgatgg agccgcaagc gttttccaga gtcagcttga ctggaatctt tgacatgtgt 3180
gtggaagcat ggaagatctt aagatatgat gataccaaat ccagatgccg cgcatgcgag 3240
tgcgggggca ggcatgccag gttccaacct gtatgtgtgg aggtgaccga ggagctgaga 3300
ccagatcatt tggtgctgac ctgcactggt gcggagttcg gttccagtgg tgaagaaact 3360
gattaaagtg agtagtggga tgttataaaa gtgaccataa ggtgatgtga gatggacaaa 3420
tttggtaatt tttatgtatt tttgtcttgc agccatgagt gggagcgctt cctttgaagg 3480
gggcgtcttt agcccttatc tgacggggcg tctgcctcat tgggctggag tgcgtcagaa 3540
tgtgatgggg tctacagtgg atggaagacc tgttcagcct gctaattctt ctactctgac 3600
ttatgctact atgacttcct cgcctttgga tgcagctgca gctgctgccg cttctgctgc 3660
cgccaacact gttcggggga tggccttgga gatggggtat tatggaactg tagtggccaa 3720
caccactacc ccaaataacc ccacagcctt gaatgaggac aagctgctag ttctcatgtc 3780
ccagctggag tctttgaccc aacgcctggg cgatctagct cagcaggtgt cccagctgaa 3840
ggagcagact caagctgcaa ttaccactgc gaggggaaat taaaaaaatt caaagaatca 3900
ataaataaac cgagactttg ttgattttaa agtgtgtcat tctttattta atttttcgcg 3960
cgcgatatgc cctggaccac cggtctctat cattgaggac acggtggatc ttttctagaa 4020
cccgatagag gtgggattgg atgttgaggt acatgggcat aagaccatct ttggggtgta 4080
gatagctcca ctgcagagcc tcatgctccg gggtggtgtt gtatataacc cagtcatagc 4140
atgggcgttg ggcatgatgt tgcacaatat ctttaaggag gagactaatg gccactggga 4200
gacccttggt gtaagtgttt acaaatctat taagctggga cgggtgcatc cgaggtgaga 4260
taatgtgcat tttggattgg atttttagat tggcaatgtt tccccctaga tctctcctgg 4320
gattcatgtt atgcaagacc actagaacag tgtatccggt gcacttaggg aatttgtcat 4380
gaagtttgga ggggaaagca tgaaaaaatt tagacacacc cttgtgtcct cccaagttct 4440
ccatgcactc atccataata atggcaatgg gcccatgggc ggcggcacgg gcgaacacgt 4500
tcctgggatc tgacacatca tagttgtggt cttgggtcag gtcatcataa gccattttaa 4560
taaacttggg gcggagggtg ccagattggg ggatgaatgt tccctcgggc cccggaacat 4620
agtttccttc acatatttgc atttcccagg cttttagttc agaggggggg atcatgtcca 4680
cctgtggagc gatgaagaag acggtctcgg gggcgggggt gattaagtgg gaggacagca 4740
agttcctaag cagctgtgac ttgccacacc cagtgggacc gtagatgacc cctataacag 4800
gttgcagatg gtagtttagg gaaagacagc tgccgtcctc tcgcaggagg ggggcgacct 4860
cgttcatcat ttccctcaca tgcatgtttt cccgcacaag ttccgatagg aggcgctctc 4920
cacccaggga aaggagttct tgaagagatg agaaattttt caagggtttt aagccatcag 4980
ccatgggcat tttggagagg gtttgttgca agagttcaag gcggtcccag agttcggtga 5040
tgtgttctat ggcatctcga tccagcatac ttcctcgttt ctggggttgg gacggctgcg 5100
ggagtatgga accaggcgat gggcgtccag cgctgccagt gtccggtcct tccacggtcg 5160
cagcgtccga gtcagggtcg tttccgtcac ggtgaagggg tgcgcgcctg gctgggcgct 5220
tgcgagggtg cgcttcaggc tcatcctgct cgtggagaac cgctgccgtt ctgcgccctg 5280
tgcatcggcc aggtagcaat taaccatgag ttcgtagttg agcgcctctg ccgcgtggcc 5340
tttggcgcgc agcttacctt tggaagtctt ctgacaggtg ggacagtaga gacacttgag 5400
agcatagagt tttggggcta gaaagaccga ttctggggag tatgcatcgg ccccacagga 5460
ggcgcagacg gtttcgcatt ccaccagcca tgtaagatcg ggctcgttgg ggtcaaaaac 5520
aagttttccg ccatgttttt tgatgcgttt cttacctttg ctttccatga gttcgtgccc 5580
ccgttgggtg acaaagaggc tgtccgtgtc cccgtagact gactttatgg gcctgtcctc 5640
gagcggcgtg ccgcggtcct cttcgtagag gaactcggac cactctgaga cgaaagcacg 5700
tgtccaggcc agcacaaagg aggctatatg ggaggggtag cgatcgttgt caaccaaggg 5760
gtctactttt tccaaggtgt gtaaacacat gtccccttct tccacatcca ggaaggtgat 5820
tggcttgtaa gtgtatgcca cgtgacctgg ggtcccagac gggggggtat aaaagggggc 5880
gggtctctgc tcgtcctcac tgtcttccgg atcgctgtcc aggagcgcca gctgttgagg 5940
taggtattcc ctctcgaagg cgggcataac ctccgcactc aggttgtcag tttctaggaa 6000
cgaggaggat ttgatattga cagtgcctgc cgagatgcct ttcatgagac tgtcgtccat 6060
ttggtcagaa aagacaatct ttttgttatc aagtttggtg gcgaaggatc catacagggc 6120
attggaaagc agtttggcaa tggagcgcat ggtttggttt ttttctttgt ctgcgcgctc 6180
tttggcggct atgttgagtt ggacatattc gcgggccaga catttccatt gtggaaatat 6240
ggtagttaat tcatctggga cgattctgac tttccagcct ctgttatgca gggtaatcag 6300
atccacactg gttgccactt ctcctctaag tggttcatta gtccagcata gtcgcccccc 6360
ttttcgagaa cagaaagggg gtaggggatc tagcatgagt tcgtctgggg ggtctgcatc 6420
tatggtgaaa atcccaggaa ggagatcttc gtcaaaatag ctgatggtgg cggggtcatc 6480
cagagacatt tgccattctc gagcagccag agcgcgctcg taggggttaa ggggagtccc 6540
ccatggcatg ggatgggtga gtgcagaagc atacatgcca cagatgtcat agacatagag 6600
cggctcttcc agaatcccta tgtaagtggg ataacatcgc ccccctctga tgctggctcg 6660
cacataatca tagagttcat gtgagggcgc tagaagaccc gagcccaggt tggtgcggtt 6720
gggtttttct gctctgtaga ggatctggcg aaagatggca tgggagtttg atgagatggt 6780
gggtctttgg aagatgttga aatgggcatg aggcagtccc acagagtccc ttatgaagtg 6840
agcataggag tcttgcagtt tggccaccag ctcggcggtg accagcacat ccaaagcaca 6900
gtagtcgagg gtctctttga tgatgtcata gttaggttcc cctttctttt cccacagctc 6960
gcggttgaga aggtattctt cgcgatcctt ccagtactct tcgaggggga acccgtcctt 7020
gtctgaacgg taagaaccca gcatgtaaaa ttgattgaca gctttgtagg cacaacaccc 7080
cttctccacg gggagtgagt atgcttgcgc ggctttgcgc agagaggtgt gagtaagggc 7140
gaaagtgtcc ctgaccatga ctttgaggaa ctgatgctta aagtctatgt catcgcaggc 7200
cccctgctcc cacagttgga agtccactcg ctttttgtag gcgggattgg gcaaagcgaa 7260
agtaacatcg ttgaatagga tctttccagc cctgggcatg aagttgcgag taatgcgaaa 7320
aggctgaggc acttctgccc tgttgttgat aacttgggca gccaagacga tctcgtcaaa 7380
gccgttgatg ttgtgaccca caatgtacag ttctacgaag cgtgggcgtc ccttgatgtg 7440
gggcagtttt ttaagctctt cgtaggtcaa gtcgtcaggg tcagcgattc catattgctc 7500
caaagcccag tcaggcaggt gaggattagc atgaaggaaa gaggtccaaa gatccacggc 7560
cagagctgtt tgtaagcggt ctctgtactg acggaaatgt cggcctaccg ccattttttc 7620
aggagtaaca cagtaaaagg tgcgcgggtc cttttcccag cgatcccatt gaagttgcaa 7680
ggctaggtcg tgggcgaggt tgacgagctg ttcgtccccc gaaagtttca tgaccagcat 7740
gaaagggaca agctgcttgc caaaggaccc catccaggtg taggtttcca catcgtaggt 7800
gaggaagagc ctttctgtgc gaggatgaga accgatcggg aagaactgga tttcctgcca 7860
ccagttggag gaatggctgt tgatatgatg gaagtagaac tccctacggc gcgccgagca 7920
ttcgtgcttg tgcttgtaca gacggccaca gtactcgcag cgctgcacgg gatgcacctg 7980
atgaatgagc tgtacctggc ttcctttgac aagaaatttc agtgggaagt tgaggcgtgg 8040
cgtctgcatc tcgtgttgta ttacgtcctg gctattggtc tggccatctt ctgtctcgat 8100
ggtggtcatg ctgacgagcc cgcgcgggag gcaggtccag acctccgcgc ggacgggtct 8160
gagagcgagg acgagagcgc gcaggccgga actgtccagg gtcctgagac gctgcggagt 8220
caggtcagta gggagagtac ataggtttac ttgcataagt ttttccaggg catgtgggag 8280
gtcaagatga tatttgattt ctactggcga gttggtggag acatcgatgg cttgcagggt 8340
cccgtgcccc tggggtgcta ccaccgtccc tttttttttc ttgatcgggg gcggtgttgc 8400
ttcttgcatg gtaaggtcgt cttctagaag cggcggcgag gtcgcgcgcc gggtggcagt 8460
ggcggttctg gacctggagg tagaggcggt agaggtacgt cggcgccgcg cgcgggtagg 8520
ttctggtact gcgccctgag aagacttgcg tgagcgacga cgcggcggtt gacgtcctgg 8580
atctgacgcc tctgggtgaa tgctaccgga cccgtgagct tgaacctgaa agagagttca 8640
acagaatcaa tttcggtatc gttgacggct gcctgccgca ggatttcttg tacgtcgccc 8700
gagttgtctt ggtaggcgat ctcggccatg aactgctcga tctcttcttc ttggagatct 8760
ccgcggcccg ctcgttctac ggtggcagca aggtcgttgg agatgcgccc catgagctgt 8820
gagaatgcat tcatgcccgc ctcgttccag acgcgactgt agaccacggc tccctcggga 8880
tctctggcgc gcatgaccac ttgggcgagg tttagttcca cgtgtctggt gaagaccgca 8940
tagttgcaga gacgctggaa gaggtagttg agcgtggtgg cgatgtgctc ggtgacaaag 9000
aaatacatga tccagcgacg aagcggcatc tcgctgatat cgcccagggc ttccaaccgt 9060
tccatggctt cgtaaaagtc cacggcgaag ttgaaaaact gggagttgcg agcggacacg 9120
gtcaactcct cctccagaag acggatgagc tcggcgatgg tggcgcgcac ttcgcgctca 9180
aaggctcccg ggatctcttc ctcctcttct tcttccaact cttcctccac taacatctct 9240
tctacttcct cctcaggcgg cgggggtgga ggagggggcg cgcggcgacg ccggcgacgc 9300
acgggcagac gatcgatgaa gcgttcgatc acttctccgc ggcggcgacg catggtctcg 9360
gtgacggcgc gcccgtcctc cctgggtcgc agagtgaaga cgccgccgcg cagctccctg 9420
aaatggtgac tgggagggtc cccgtttggt agggacaggg cactgatgat gcatcttatt 9480
aattgccctg tagggactcc gcgcaaggac ctgagcgtct cgagatccac gggatctgaa 9540
aatctctgaa cgaaggcttc gagccagtcg cagtcgcaag gtaggctgag cactgtttct 9600
tcggggcggg ctgctgagct agagggttgt acgatgctgc tggtgatgaa gttaaaatag 9660
gcagttctga gacggcggat ggtggcgagg agcaccaggt ctttgggtcc ggcttgctgg 9720
atgcgcaggc ggtcggccat tccccatgca ttatcttggc acctggccag atctttatag 9780
tagtcttgca tgagtcgctc cacgggcact tcttcttcgc ccgctctgcc gtgcatgcgt 9840
gtgagtccgt accctctctg tggttggacg agcgccaggt cggcaacgac cctttcggct 9900
agaatggctt gctgcacctg ggtgagggtg ttctggaaat catcaaagtc cacaaagcgg 9960
tggtaggccc ccgtgttgat ggtgtaggag cagttggaca tgaccgacca gttgactgtc 10020
tggtgtcctg gtcgtacgag ttccgtgtac ctgagccgcg agtatgcgcg ggagtcgaag 10080
atgtaatcgt tgcaggttcg caccaggtac tggtagccga tgaggaagtg aggcggcggc 10140
tggcggtaga gaggccatcg ttcggtggcg ggcgcgccgg gcgctaggtc ttctagcatg 10200
agacggtggt atccgtagac gtacctggac atccaggtaa taccggcggc ggtggtggag 10260
gcgcgcggaa actctcgcac gcggttccag atgttgcgca gcggcatgaa gtagttcatg 10320
gtgggcacgg tctggcccgt gaggcgcgcg cagtcattga tgctctagat acgggcaaaa 10380
acgaaagcgt tgagcggttc ccttccgtgg cctggaggaa cgcgaacggg ttaggtcgca 10440
gcgtaccctg gttcgagact aaagaaagcg agcaactcga accggcagag tcgcggctaa 10500
cgggtattgg caatcccgtc tcgacccaag ccagcaaatc caggatacgg atgggggccc 10560
cttttgtttt tcagggcatg agtcaccggt taaggtttac aacggctgtt tcatgccttt 10620
agaagtggct cgcgcccgta gtctggagaa tcaatcgcca gggttgcgtt gcggcgtgcc 10680
ccggttcgag cctgcagctt gagtcggccg gtgaccgcgg caaacgaggg cgtggcggcc 10740
ccgtcgtttc taagaccttg ctagccgacc tctccagttt acgggaacga gccccctttt 10800
attttttttg tttttgccag atgcatcccg tactgcggca gatgcgccca cagcccccac 10860
agcagcagca gcaggctggc ctaccttctc tacctcagcc gctacctgca actaccgcgg 10920
tggccgctgt aagcggggcc ggacagcagg cggctcctca atatgaattg gacttggaag 10980
agggcgaggg attggcaaga ttgggggcgc cctcgcccga gcgccacccg cgggtgcaga 11040
tgaaaaagga cgttcgcgaa tcttacgtgc ccaagcagaa tctgttcaga gacagaagcg 11100
gcgaggagcc cgaggagatg cgcgcgtccc gttttaacgc gggtcgcgag ctgcgacaag 11160
gactggatcg aaaacgggtg ttgagggatg atgattttga ggtggatgaa atgacaggga 11220
tcagccccgc tcgcgctcac gtggctgcag ctaatctggt gacagcttat gagcagaccg 11280
tgaaggagga aagcaacttc cagaaatcat tcaataacca cgtgcgcacc ctgatcgcac 11340
gcgaggaggt gaccctgggc ctgatgcacc tgtgggatct gctggaagcc atagtgcaga 11400
accccactag caaacccctg actgctcaac tgtttctggt ggtgcagcac agcagggata 11460
atgaggcatt cagagaggcg ctgctgaata tcactgaacc tgaggggaga tggctgctgg 11520
atctggtgaa tatcctgcag agcattgtag tgcaggaacg cagcttgcct ttgtccgaga 11580
aggtggcggc gatcaattac tctgtgctga gtctgggcaa atactatgcc aggaagatct 11640
acaaaacccc ttacgtgccc atagacaagg aagtgaaaat agatgggttt tacatgcgca 11700
tgaccctgaa agtgctaacc ctgagcgatg acttgggagt gtaccgcaac gacaggatgc 11760
accgcgcggt gagcgccagc aggaggcgcg agctgagcga caaagaatta atgcacagct 11820
tgcaacgagc cctgacggga gccgggacgg agggggagaa ctactttgac atgggtgcag 11880
acttgcattg gctgcctagt cgcagggcat tggaagcggc aggcgatggg ccctatgtag 11940
aggaagtagt agacgaggac gatgaggagg gcgagtacct ggaagactga tggcgcgacc 12000
cgtatttttg ctagatggaa caggcgccgg accctgcgat gcgggcggcg ctgcagagcc 12060
agccgtccgg cattaattcc tcggacgatt ggacccaggc catgcaacgc atcatggcgc 12120
tgacgacccg caaccccgaa gcctttagac agcagcctca ggccaaccgc ctttcggcca 12180
tcctggaggc cgtggtgccc tctcgctcca accccaccca cgagaaggtt ctggccatcg 12240
tgaatgccct ggtggagaac aaggccatcc gctccgatga agccgggctg gtatacaacg 12300
ccttgctcga gcgcgtggct cgctacaaca gcagcaatgt ccagactaac ctggacagga 12360
tggtgaccga cgtgcgcgag gccgtgtccc agcgcgaacg gttccatcgc gagtctaacc 12420
tgggttccat ggtagcgctg aacgctttcc tcagttccca gcctgccaat gtgccccggg 12480
gacaggaaga ctataccaac tttattagcg ccctgagact catggtagcc gaggttcctc 12540
agagcgaggt gtaccagtcc ggtccagact actttttcca gacaagcagg aacggtatgc 12600
agacagtgaa cttaagccag gctttcaaga acctgcaagg gctgtgggga gtccaagctc 12660
cagtgggcga cagggcgacc gtgtcgagcc tgttgactcc aaattcccgt ttgctgctgc 12720
tgctggtgtc ccccttcact gacagcggca gcataaacag aaactcctac ttgggctacc 12780
tgataaactt gtatcgcgaa gctataggtc aggcccacgt ggacgaacag acctatcagg 12840
agatcactaa tgtgagtcgc gctctgggcc aggacgaccc tggaaacctg gaagctactc 12900
taaactttct gctgaccaac cgctcgcaaa aaatccctcc tcagtataca ttaactgcgg 12960
aggaggaacg gatcttgaga tacgtgcagc agagcgtggg tctgttcctg atgcaagagg 13020
gtgcgacccc tagcgccgcg cttgatatga cagcgcgcaa catggagccc agcatgtatg 13080
ccagcaacag accattcatt aataaattga tggattactt ccatcgcgcg gccgctatga 13140
actctgatta cttcaccaat gctattctga acccccattg gctgcctccg cctggttttt 13200
atactggcga gtatgacatg cctgacccca acgatgggtt cttgtgggac gatgtggaca 13260
gcgtggcgtt ctcgcctacc gctcctcgta ctttttggaa gaaggaaggt agtgacagaa 13320
gaccctcctc cgtgctgtca ggacgtgagg gtgctgccgc ggcggtcccc gatgctgcaa 13380
gcccctttcc cagtctgcca ttttcactaa acagcgtgcg cagtagcgag ctggggagaa 13440
taacccgccc tcgcttgctg ggcgaggacg agtatttgaa tgactcccta ctgagacccg 13500
agcgggaaaa gaacttccct aataatggga ttgaaagcct ggtggataag atgagcagat 13560
ggaagaccta tgcccaggag cacagagatg agcctagaat cttgggtcct acagtaggca 13620
cccgcagacg ccagcgccat gatagacagc ggggtctggt gtgggacgat gaggattctg 13680
cagatgacag cagcgtgttg gacttgggcg ggaggggagg tgtgggcaac ccgttcgcac 13740
acttgcgtcc ccgtattgga cgcatgatgt aaaagtgaaa ataaaaaagg aactcaccaa 13800
ggccatggcg accagcgtgc gttcgttctt tctgttgttg tatctagtat gatgaggcgc 13860
accgtgctag gcggatcggt ggcgtatccg gagggtcctc ctccttcgta cgaaagcgtg 13920
atgcagcagg tggcggcggc ggcgatgcaa ccccccttgg aggctcctta cgtgcccccg 13980
cggtacctgg cacctaccga ggggagaaac agcattcgtt attcggaact cacacccttg 14040
tatgacacca cccggttgta cctggtggac aacaaatcgg cggacattgc ctcgttgaac 14100
tatcagaacg accacagcaa cttcttgaca acggtggtgc agaacaatga ctttaccccc 14160
acggaggcca gcacccagac catcaacttt gacgagcgct cccggtgggg cggtcagctg 14220
aagaccatca tgcacaccaa catgcccaac gtgaacgagt tcatgtttag caacaagttc 14280
agggctaggg tgatggtgtc cagaaccaca cctaaagagg tgacagtcac aacagactat 14340
gatggtagtc aggacatctt ggaatacgag tgggttgact ttgagttacc agaaggcaac 14400
ttctctgcca ccatgaccat agacctgatg aataatgcaa ttgttgataa ttacctaaaa 14460
gtgggtagac agaatggggt actggagagt gacataggtg ttaagtttga cactaggaac 14520
tttaggcttg gttgggaccc agtgacagag ttggtcatgc ctggggtcta caccaatgaa 14580
gctttccatc ctgacatagt cctactacct ggctgcggag tggacttcac tgagagccgc 14640
ctcagtaatc tgctaggcat tagaaagaaa cagccattcc aggaagggtt ccagatcatg 14700
tatgaggatc tggagggtgg taacatcccc gccctgcttg atgtaaatgc atatgagaag 14760
agcaaggaag ataatacaac caccacaaat gaagctgtgg ccgcggcttc atctactgaa 14820
gccaaagctg tggtagatgc ttccacttca acagaaaaca ccactgatga aaaagtcacc 14880
aggggagata catttgccac ccctgaacaa gagaaggcag ctgaggcaga gtctgatatt 14940
atgcttctgt ccaccgatga aaacgaaact aaaaaacaac tggttattcg agcggtgacc 15000
aaggatagta aggacaggag ttataatgta ttgtcagatg gaaagaacac agcttaccgt 15060
agttggtacc tggcatacaa ttatggcgac cgtgagaaag gggtgcgttc ttggacactg 15120
cttaccacct cggatgtcac ctgcggcgtg gagcaagtct attggtcgct accagatatg 15180
atgcaagatc cagtcacctt tcgctccaca cgccaagtta gcaactaccc agtggtgggc 15240
gcagagctgc tcccagtgca ttccagaagc ttctacaacg agcaagccgt ctactcgcaa 15300
cagctccgcc agtacacctc gctcacgcac gtcttcaacc gcttccccga gaatcagatc 15360
ctcgtccgcc cgcccgcgcc aaccattacc accgtcagtg aaaacgttcc tgctctcaca 15420
gatcacggga ccctgccgct gcgcagcagt atccggggag tccagcgcgt gaccgttact 15480
gacgccagac gccgcacctg tccatacgta tacaaggccc tgggcatagt cgcgccgcgc 15540
gtcctttcaa gccgcacttt ctaaaaaaat gtccattctc atctcgccca gtaataacac 15600
cggttggggc ctgcgcacac ctagcaagat gtatggaggc gctcgcagac gctccactca 15660
gcaccctgtg cgcgtgcgcg ggcatttccg cgctccctgg ggcgccctca agggacgctc 15720
tcgtactagg accaccgttg acgatgtgat cgaccaggtg gtcgccgatg cacgtaacta 15780
tacccccgca gccgcacctg catccaccgt ggatgcggtc attgacagcg tggtagccga 15840
tgcgcgcgcc tatgctcgcg ccaagagcag gaggcggcgt attgccaggc gtcaccgagc 15900
tactccagcc atgcgagctg caagagcttt attgcggaga gccagacgtg tggggcgaag 15960
agccatgcgt agagcggcca gacgcgcggc ttcaggtgcc agcgcaggca gggtccgcag 16020
gcgcgcggct acggcggcag cggcggccat cgctagcatg accaaaccac gaagaggcaa 16080
tgtgtattgg gtgcgcgacg ccgccaccgg ccagcgcgtg cccgtgcgca cacgcccccc 16140
tcgcacttag aagatactga gcagtctccg atgttgtgtc ccagcggcga gatgtccaag 16200
cgcaaattca aggaagagat gctccaggtc atcgcgcctg agatctacgg tcctgcggtg 16260
aaggatgaaa aaaagccccg caagatcaag cgggtcaaaa aggacaaaaa ggaagaagat 16320
ggtgatgatg ggctggtgga gtttgtgcgc gagtttgccc caaggaggcg cgtgcagtgg 16380
cgcgggcgca aagtgtggcc ggtgttgaga ccggggacca cagtggtctt tacgccaggc 16440
gagcgctcca gcaccgtttc caaacgctct tatgatgagg tgtacgggga cgatgatatt 16500
ctcgagcagg cggctgatcg ccttggcgag tttgcatatg gcaaacgcag ccgctcggga 16560
gccaaggaag aggcattgac catccccttg gatcatggaa atcccacccc aagcctcaaa 16620
cccgtgaccc tgcaacaagt gctgcccacg ccgccacgca agggcatcaa gcgcgagggc 16680
gaggatctgt atcccaccat gcagctgatg gtgcccaagc gccagaagct ggaagacgtg 16740
ctggagaaaa tgaaagtgga tcctgaaatc cagcctgaag tcaaagtgag gccaatcaag 16800
caggtggcgc ccggtttggg ggtacaaacc gtggatatca agatccccac cgagtccatg 16860
gaaattcaaa ccgaacccat gaagcccacc tccagcacca ttgaggtgca gacggatcct 16920
tggatgcccg cgcctgctcc tgttaccact actactcgaa gacctagaag aaagtatggt 16980
tcagccaacc tgataatgcc aaactatgct ctgcatccat caatcatacc cactcctggc 17040
taccgcggca ctcgctacta ccgcagtcac agcacccgcc gacgtaaagc acctgccacc 17100
cgccgccgtc gccgccgccg tgccactagc aaacttaccc cctcggctat ggtgcggaga 17160
gtgtaccgtg atgggcgcgc agctcctctg acactgccgc gcgcgcgcta ccatcctagc 17220
attgccattt aacaactctg cctccttgca gatatggccc tcacttgccg ccttcgtatt 17280
cctattgctg gctaccgcgg aagaaagtcg cgccgtagaa gagcagggtt gtctgggagc 17340
gggatgcgtc gccaccggcg gcggcgcgcc atcagcaaac ggttgggggg tggatttctt 17400
cccgctttga ttcccatcat cgccgcggcg atcggcgcga taccaggcat agcttccgtg 17460
gcggtgcagg cctcgcagcg ccactgacat tggaaaaaga tatcttataa ataaaaatag 17520
aatggactct gacgctcctg gtcctgtgat atgtttttgt agacgagatg gaagacatca 17580
atttttcatc cctggctccg cgacacggca cgcggccgta tatgggcacc tggagcgaca 17640
tcggcaacag ccaactgaac gggggagcct tcaattggag cagtctatgg agcgggctta 17700
aaaattttgg gtccactata aagacttatg ggaacaaagc ttggaacagc agcacagggc 17760
atgcgctgag acaaaagctt aaagatcaga atttccaaca gaaggtggtc gatggtatcg 17820
cctctggaat caatggggtg gtagatctgg ccaaccaggc cgtgcagaaa cagattaaca 17880
gtcgcctgga cccggctccc ccagctccta ttcatgagtt aatgcaagtg gaggaagagc 17940
tcccttcatt ggaaaagcgg ggcgataagc gacctcgtcc agatatggag gaaacgctgc 18000
tgaccaaggt ggatgagccg ccctcctatg aagaggctgt aaaactggga atgcccacta 18060
caaagcccat tatgcctctg gccactggag tgatgaagcc atctcagtct aaacctgcag 18120
ttgctgctac attggacttg cccgctcccg tggccacccc caaacctgtc gccgccccga 18180
agcccaccgc cgtgcaaccc gtggccgtgg ccagaccgcg tcccggtggt cggccgaatg 18240
caaactggca gagcactctg aacagcatcg tgggtttggg agtgcacagt gtgaagcgcc 18300
gtcgctgcta ttgattaaat atggagtagc gcttaacttg cttgtctgtg tgtgtatatg 18360
tcgatgccgc ccgccgtgct acagcaaaga gagaaggaga agaggcgccg ctgagttcct 18420
ttcaagatgg ccaccccatc gatgctgccc cagtgggcgt acatgcacat cgccggacag 18480
gacgcttcgg agtacctgag tccgggtctg gtgcagttcg cccgcgccac agatacctac 18540
ttcaatctgg ggaacaagtt taggaaccct accgtggctc ccacccacga tgtgaccacc 18600
gaccgtagcc agcgcctgac gctgcgcttt gtgcccgttg accgggagga caatacctac 18660
tcctacaaag tcagatacac cctggctgtg ggagacaaca gggtgttgga tatggccagc 18720
acctactttg acatcagggg cgtgttggac agaggaccta gcttcaaacc atactctggc 18780
actgcctaca actccctggc tccaaaagga gctccaaact ccagtcagtg gcaacaaaag 18840
gaaaacaatg gtcaaggtga tgcaaagact cacacctatg gtgtagctgc cactggaggt 18900
attgacattg acaaaaatgg tcttcaaatt ggaatcgatg aaactaaaga agataataac 18960
gaaatttatg cagacaaaac attccaacct gaacctcaaa ttggagaaga aaactggcaa 19020
gatagcgaaa actattatgg aggcagggct cttaaaccgg aaaccaagat gaagccttgc 19080
tatggttcct tcgctagacc aactaatgca aagggaggtc aagccaaaat taaaccagct 19140
caagagggtc aacagtctat agattatgac atagacctgg ctttctttga tattccaagc 19200
actggcggag gcaatggcac aaatgtaaat gacaagccag atatggttat gtatactgaa 19260
aatgtaaatc tggaaactcc agacactcat cttgtttaca agccaggaac ttcagatgac 19320
agttccgagg ccaatttaac tcagcaagcc atggctaaca gacccaacta tattgggttt 19380
agagataact ttattggcgt catgtactac aacagcactg gcaacatggg agtgcttgct 19440
ggtcaagcat cccagctaaa tgctgtggtg gacctgcaag acagaaacac cgagctgtct 19500
tatcagctat tacttgactc tctgggcgac agaaccaggt attttagtat gtggaatcag 19560
gcggtggaca gctatgatcc tgatgtgcgc attattgaaa accatggtgt ggaagatgaa 19620
ttgccaaact attgcttccc attggacgga gctggcacta atgctgttta ccaaggagtt 19680
aagacaaaag aggataataa tggcgaatgg gaaacagaca caaatgttgc atcgcagaat 19740
cagatatgca agggcaacat atatgctatg gagatcaacc tgcaagccaa cctgtggaaa 19800
agtttccttt actccaacgt ggctctgtac ctaccagact cctacaagta cactccatcc 19860
aacgtgacac tccctaccaa cactaacacc tatgactaca tgaatggcag ggtggtgtct 19920
ccatccctgg tggatgccta cattaacatt ggcgccaggt ggtctctgga tgccatggac 19980
aatgtcaacc ctttcaacca ccaccgcaat gccggcctgc gctaccggtc catgcttctg 20040
ggcaacggcc gatacgtgcc cttccacatc caagtgcccc agaaattctt cgctatcaag 20100
aacctgctgc ttctcccagg ctcatacacc tacgagtgga acttccgcaa ggatgtcaac 20160
atgatcctgc agagttccct tggcaatgac ctcagaaccg atggggccac catccagtac 20220
accagcatca atctctatgc caccttcttc cccatggctc acaacactgc ctccaccctg 20280
gaagccatgc tgcgcaatga caccaatgac cagtccttca atgactacct ctcagctgcc 20340
aacatgcttt accccatccc tgccaatgcc accaacgtgc ccatctccat cccatctcgt 20400
aactgggctg ccttcagggg ctggtctttc acccgcctca agaccaagga gaccccatct 20460
ctgggatcag ggttcgatcc ctacttcgtc tactcaggct ccattccata cctggatgga 20520
actttctacc ttaaccacac tttcaagaaa gtctccatca tgtttgactc ttctgtcagc 20580
tggccaggca atgacaggct gctgactccc aatgagttcg aaatcaagcg cactgttgat 20640
ggggaagggt acaatgtggc acaatgcaac atgaccaaag actggttcct ggttcagatg 20700
ctctcccact acaacattgg ctaccagggc ttctacatcc cagaaggata caaggaccgc 20760
atgtactcct tcttcagaaa cttccagccc atgagccgcc aggtggtcga tcaggtcaac 20820
tacaaagact acatggcagt cacccttgcc tatcagcaca acaactctgg ctttgtgggc 20880
tacctcgcgc ccaccatgcg acagggccaa ccctaccctg ctaactaccc atacccgctc 20940
attggcaaga ctgcagtcaa cagtgtcacc cagaaaaagt tcctctgcga cagggtcatg 21000
tggcgcatcc ccttctccag caacttcatg tccatggggg cccttaccga cctggggcaa 21060
aacatgcttt atgccaactc cgcccacgcg ctagacatga atttcgaagt agaccccatg 21120
gatgagtcca cccttctcta tgttgtcttc gaagtcttcg acgtggtcag agtgcaccag 21180
ccccaccgcg gcgtcatcga agctgtctac ctgcgcaccc ccttctcagc tggtaacgcc 21240
accacataag cgccttgctt cttgcaagtg gctgcagcag catggcctgt ggatcctcca 21300
ctggatccaa tgagcaagag ctcagggcca tcgccataga cctgggctgt ggaccctatt 21360
tcctgggaac ctttgacaag cggtttccag gcttcatggc tcctgacaag ctcgcctgtg 21420
ccattgtcaa cacggcaggg cgcgagactg gtggtgagca ctggctggct tttggatgga 21480
acccccgctc caatacctgc tatctctttg acccgtttgg gttttcagac gagcgcctca 21540
agcagatcta tcaattcgag tacgaggggc tcctgcgccg cagtgccctg gctactaagg 21600
accgatgcat cactctggaa aagtctaccc agaccgtgca gggtccgcgc tcggctgcct 21660
gcgggctctt ctgctgcatg ttcctccatg cttttgtgca ctggcccgac cgccccatgg 21720
acaacaaccc caccatgaat ttgctgacgg gggtacccaa caacatgctc caatcgcccc 21780
aagtagagcc caccctgcgc cacaaccagg aggcactcta tcgcttcctg aactcccact 21840
catcttactt tcgttctaac cgcgcgcgca ttgagaaggc cactgccttc gatcgaatga 21900
ataataacat gtaaaccaaa ttgtgtgtgg ctcaaataaa cagcacttta ttgtttacat 21960
gcactgaggc tctgggatga tcatttttta aaaatcgaag gggttctggc gggaatcagc 22020
atggccagat ggcagggaca cgttgcggaa ctggaacttg ttctgccact tgaactcggg 22080
aatcaccagc ctgggaactg gaatctctgg aaaggtatct tgccatagct ttctggtcag 22140
ttgcagagcg ccaagcaggt caggagcaga tatcttgaaa tcacagttgg ggccagaatt 22200
ctgggcgcgg gagttgcggt acactgggtt gcagcactgg aacaccataa gggcagggtg 22260
tctcacgctc gccagcacgg tctcgtcact gatgcaagac acatccaggt cttcagcatt 22320
ggccattcca aagggggtca tcttgcaggt ctgtctgccc atcacgggag cgcagccagg 22380
tttgtggttg caatcacaat gaagggggat cagcatcatc ttggcctggt cgggggtaat 22440
ccctgggtaa acagccttca tgaaggcttc atactgcttg aaagcttcct gggctttggt 22500
tccctcggtg tagaacactc cacaagactt gctggaaaac tgattagtag cgcagttggc 22560
atcattcaca cagcagcggg cgtcgttatt agccagctgg accacattcc tgccccagcg 22620
gttctgggtg atcttggctc gatctgggtt ctccttcaac gcgcgctggc cgttctcgct 22680
cgccacatcc atctcaatga catgttcctt ctggatcatg atgttgccat gcaggcatct 22740
aatcttgcct tcataatcag tgcagccatg aggccacagc gcgcacccgg tgcactccca 22800
attgttatgg gggatctggg aatggctatg aaccagccct tgcaggaatc ttcccatcat 22860
cacagccagg gtctttatgc tggtaaaggt cagcgggata ccgcggtgct cctcgttcac 22920
atactgctgg cagatgcgtc tgtagtgctc ggcctgctcg ggcatcagct tgaaagaggt 22980
tttcaactca ttatccagcc tgtatctctc catcatgatg gacattactt ccatgccctt 23040
ctcccaggca gaaacaatag ggagactcag gggattcttg acagtagaga caaccttact 23100
taaggggtca tcactgccaa tcttttcgat gcttctcttg ccatccttct cggtgatgcg 23160
caccggcggg tagctgaatc ccacagccac caactgagcc tcttcccttt cgtcttcgct 23220
gtcttgactg atgtcttgca gaggaacatg tttggttttc ctgggtttct tcttgggcgg 23280
cagctctgga ggactctggc tccgttccgg agaccccatg gatgagcgag agttgtcgct 23340
caccacttgg atctggctgc ctgtagaaga actggacccc acgcggcggt aggtgttcct 23400
cttggtaggc agaggtggag gcgacgggct ccggtccggt ctgggtggcg gatggctggc 23460
ggagcccctt ccgcgttcgg gggtgcgctc cagatggcgg tcgtctgact gacctccgcg 23520
gctggccatt gtgttctcct aggtagagaa acaagacatg gagactcagc catcgctgcc 23580
atcgccatcc accaccacaa gcaccgccga ggaggaggag tgtttaacca ccccaccatg 23640
cagccccgct accaccacca gcacccttga aagcgaggtc gacacggtcg tggaggattt 23700
acaggctatg gaagatattg aggcagctgt cgagcaagac cccggctatg tgacaccggc 23760
ggagcatgat gaggatctag cgcgctttct cgacggtgtg gagaaagcga aacaagatga 23820
ggacgaggaa gaggcagaag cacaaccatc ggtggccgac tacctcaccg gcctagggct 23880
agaagacgtg ctgcttaagc atcttgcaag gcagacagtc atagtcaaag acgccctgct 23940
agagcgctcc gaggtgccac tcagtgtgga agacctcagt cgcgcctatg agctaaacct 24000
cttctcgcct cgcaagcccc ccaagcgtca gcccaacggg acctgtgagc ccaatccgcg 24060
cctcaacttc tatccagcct tcactgtgcc cgaagtacta gctacctacc acatcttttt 24120
caagaaccaa aagatcccca tctcctgccg cgccaatcgc acccgcgcag atgccctact 24180
caacttgggg cccggcgctc gcatacctga tatcgcttcc ttggaagagg ttcctaagat 24240
ctttgagggt ctgggcaatg aggaaactcg ggcagcaaac gctctgcaaa gagaaacaga 24300
tgatggtgaa caccacagcg ctctggtgga gctccagggc gacaacgctc gtcttgcagt 24360
cctcaaacgc agcatcgagg tcacccattt cgcctacccc gcacttaatc tcccacccaa 24420
agtcatgagc tcggtcatgg acacgttgct catgaagcgc gcgagcccca tctccgagga 24480
tcagaacatg caggaccccg atgcctcaga tgaaggcaag cctgtagtca gcgacgagca 24540
actggctcgc tggctaggct ctgactcccc ccagtctttg gaggagcggc gcaagcttat 24600
gatggcagtg gtcctgatca cagcggagct ggagtgtctc cgccgcttct tcactgaccc 24660
agagaccctg cgcaagcttg aggagaacct gcattacaca ttcagtcatg ggttcgtgcg 24720
ccaggcgtgc aagatctcca acgttcaact caccaacctg gtctcctacc tgggcatctt 24780
gcatgaaaac cggctggggc agaacgtgct ccacaccacc ctgaaggggg aggcccgccg 24840
cgactatatc cgcgactgta tctacctcta cctatgctac acctggcaaa gcgggatggg 24900
tgtgtggcaa cagtgcttgg aagagcaaaa tctaaaagag ctggaaaagc tgcttcagaa 24960
atctcttaaa tctctgtgga ccgggttcga tgagcggacc accgcttcgg acatggccga 25020
tattatcttc cccgagcggc tcagacacac tctgcgcgac gggctgcctg actttgccag 25080
ccagagcatg ctacaaaact ttaggtcatt catcttggaa cgctccggga tcctgcccgc 25140
cacttgctgc gcactgccct ccgattttgt gcccatcacc taccgggagt gccccccgcc 25200
gctatggagc cactgctacc tgttccgcct ggccaactac ttggcctacc actctgatgt 25260
gatagaagat gttagtggcg aagggctcct ggagtgccac tgccgctgca acctctgcac 25320
cccccaccgc tccctcgcct gcaatcccca gctgctgagc gaaacccaga tcatcggcac 25380
cttcgagttg caaggtccca gcggcgaagg cgaggggtcc tctccggggc aaagtctgaa 25440
actgactccg gggctatgga cctccgctta ccttcgcaag ttcgccccca aagactacca 25500
cccctatgag atcaggtttt atgaagacca atcacagccc cccaaggccg aactgacggc 25560
ctgcgtcatc acccaggggg caatcttggc ccaattgcaa gccatccaaa aatcccgcca 25620
agaatttttg ctgaaaaagg gacacgggat ctatctagac ccccagaccg gtgaggagct 25680
gaatacacgc ttccctcagg atgccccgag gaggcaagag aatgaaagtt cagatgccgc 25740
ccgaggagga gctggaagac tgggacagtc aggcagagga ggaagactgg gacagccagg 25800
cagaagagga ggacagcctg gaggaggaca gtctggagga aggcgaggag cccaaggaag 25860
aggcagccgc cgccagacca tcgtcctcgg cggtggagac aagcaaggtc ccagacagca 25920
cggctaccac ctccgctcca gctcaagggg ccgctcggcg acccaacagt agatgggacg 25980
agacgggtcg cttccagaac cccaccaccg tcaagaccgg taagcaggag cggcagggat 26040
acaagtcctg gcgggggcat aaaagtgcca tcatcgcttg cttgcaggag tgtgggggca 26100
atatatcctt tgccagacgc tacctgctat tccatcacgg ggtgaatttc ccccgcaaca 26160
tcttgcatta ctaccgtcac ctccacagcc cctactacca gcagcaagag acagcagagg 26220
aaaccagcgg caactccgag agttagaaaa ccagcagcta aaaaatccac agcggcggca 26280
gcaggtgcag gcggactgag gatcaccgcg aacgagccag ctcagaccag ggagttgagg 26340
aatcggatct ttcccaccct ctatgccata ttccaacaaa gtcggggtca ggaacaagaa 26400
ctgaaagtaa aaaacagatc tcttcgctcg ctcacccgca gttgtttgta tcacaagagc 26460
gaagaccaac ttcagcgcac tctcgaggac gccgaggctc tcttcaacaa gtactgcgcg 26520
ctgactctta aagagtagac tgcgcgcgct tggcgagaaa aggcgggaat tacgtcacct 26580
cttggccaca cctgtgcttc attatgagta aagaaattcc cacgccttac atgtggagct 26640
atcagcccca gatgggattg gccgctggcg ccgcccagga ctactccacc cgcatgaatt 26700
ggctcagcgc cggtcccgcg atgatctcac gggttaatgg tgtgagagag caccgaaacc 26760
agatactcct agaacagtcc gccctcaccg ccactccccg caatcacctc aacccccgta 26820
attggcccgc cgccctggtg taccaggaaa ctcctgctcc cactacagta ctacttcctc 26880
gtgacgccca ggccgaagtt cagatgacta actcaggtgt acagctggcg ggtggtgcca 26940
ccctgtgtcg tcaccggcca agaccgggta taaagggcct ggtgatcaga ggccgaggta 27000
ttcagctcaa cgacgagtcg gtgaactctt cgcttggtct gcgaccagac ggcatcttcc 27060
aaatagctgg ttgtgggaga tcttccttca ctcctcgtca ggctgtcctg actttggaga 27120
gttcgtcctc gcagccccgc tcgggcggca tcgggactct ccagttcgtg gaggagttta 27180
ctccctcggt ctacttcaac cccttctccg gttctcctgg gctttacccg gacgagttca 27240
tcccgaacta cgacgccatc agtgaagcgg tcgacggcta cgattaatgt ctaatggtgg 27300
cgcggctgag ctagctcgac tgcgacacct agaccactgc cggcgctttc gctgctttgc 27360
tcgggatctc tgcgagttca tctacttcga gtaccctgac gaacatcctc agggacctgc 27420
ccacggagtt cggattacca ttgaaggggc tatcgattct cacctgcttc ggatcttcac 27480
cgctcggcca gtgctagttg agcgcaacca gggcgacacc accatctccc tctgctgcat 27540
ttgtgacaac cccggattgc atgaaagctt ttgttgtctt ctttgtactg agtataataa 27600
aagctgaaat tagagactac tccggactct cttgtcgtct gaacaacacc aaccagaccc 27660
ttcacttcag cgggaaccag actactcttc actgtaaggc ttataactat aagtatctta 27720
cttggatata caaaggaaca ccgtttgctg tggtaaacag gtgctccaac gacggtgttc 27780
tcctcacctt cctaggcaac ttctccaact ttaccttttc tgttcgcaga aacaagctta 27840
ccctccttca gccctacttt cctgggatct atacctgcct cagtggacct tgcaaccaca 27900
cttttcacct gattgaaaac tctaccctta ccttcccagc gccaatccct actaacagct 27960
cggagtccaa ctcttccatt accgctgata ctaacactcc taaaaccgga ggtgagctcc 28020
gcagccttcc cccggctgca gataaccctt gggtggtagc gggatttgta gcgctaggaa 28080
tagttgcggg tgggctcgcg ttcgtcctct gctacctata ccttacctgc tgctcatatt 28140
tagtagtact gtgctgttgg tttagaaaat gggggcgcta ctaatcacac ttgctttact 28200
ttcgcttttg ggtctgagct cggctaatag cgagaaacca agctgtctag aaacaaactc 28260
tccagactgt gtggttcctc atgggctctc agacccagct gatgatccat gcttaacttt 28320
tgacccagaa aaaaactgct cggtgactat gcagccctat gcttacatgt gcacatctgt 28380
tataaagtgc ggatggggct gtaaaccgat tgaaattacc cacaaaggca aaacctggaa 28440
taatagtttg tttaacacat ggcagcctgg agacgagcag tggtatactg tcactgtccg 28500
tggtcctgac ggctcagtta aagctcatta caatctaacg ttcatgtttg cagaattgtg 28560
tgatctggct atgatcatgc aaagacagta caggctgtgg ccccccacta aggaaaacat 28620
tgttgagttc tccattgctt actgcatagg cacctgcttg gtcactgcta tcatgtgcgt 28680
ctgcattcac ctgcttgtaa tcattagacc caggaataac aatgaaaagg aaaaaatgcc 28740
ataatctttc taactttgtt ttcagccatg attactttta caactattct tgctatcatt 28800
agcattgtga ctgctcaaag gcacccacac acaacactaa acatttacac tggtcaaaac 28860
tatacattgt ggctaacaag caactactca aatgttatgt ggatatatca tacaaaatca 28920
tggaccaaca gccaaatgct atgtcgcggt accacaactt catacccaga gcttaagcac 28980
acttgtactg acacaaactt aacacttatt aatgtaaatg caaccttcaa tgccgactat 29040
tatgcatata gcggcaccca ttctttccaa tttacaattc acgttcacaa cttaaccaca 29100
ccaaaaccac caacaacact tacaacaact acaaccagca caaccacagc cagaactaaa 29160
cctacaacag gcaagcagtc cactaaaaca attcatacac ttggtttctt ggaaacattg 29220
ttttttgcta gaagcagttc taacaactct ggaaatgcaa cactagctga tgaagctcaa 29280
attcctggtt ctatgattgg aattgttatg gcagtagtga tagtaatggt gattattatc 29340
attagcatga tcacctatgc ttgctgctat aggaaattcc atcctacaaa agttgatcca 29400
ttattaaatt ttgattttta atcctacaga acaatgaaaa agctgcttat aattttgctt 29460
ttaaaattct catttggagg aaagttatac acgcgcataa caacaactga aggacaaaac 29520
ttaacattgc taggtgtaaa tgctattaat aataatgttt acaaaactgt caacataaca 29580
tgggaaactg aaattaatgg aactaggcaa aatgtgtgca aactaaacat aacaaaatat 29640
acatgtaagg cttatgattt aataatagtt aatgttacca cacttgattc aaaattatac 29700
gtagggcaaa gctacactgt ttttgcaaaa ggattaacta aaggctatac tatacataac 29760
cttgcagcac accaagtgac agttattaaa gcaacaacac cccgcaccac aactgtcaca 29820
acacacaaaa gcaccgcttt gttacaatca atacacagta cgcctactat acctcaacca 29880
attgctgaag cagtgcactt taataatcat gaaaccaata tgcagactac tgaaaaaatc 29940
actagcaaaa aacataccac tattgctgtg cttatgccaa cacctgccaa aatctttttg 30000
atgccaatat cagttcaaat cgcaataatt gctgtaatag gcattgtgat tatttcttgt 30060
ctgctttatt atgttttctg ctgtcataaa cactatactg aaagaaaacc catttataga 30120
ccaatgatag gggatccaga acctcttaag gtggagggtg gtctaagaaa tcttctcttc 30180
tcttttacag tatggtgaac tgcaatcatg atccctaggc aatttttctt caccatacta 30240
atctgtgctt tgaatgtgtg tgcaactctc gctgtagtag ccaacacaaa cccagagtgt 30300
ctaggcccct tcgctaccta cctgctattt gtctttatta cctgcacctg catctgtagc 30360
atagtctgcc tgattattaa cttctttcaa ttcatagatt gggtatttgt gcgaatttgc 30420
taccttcgcc atcacccaga ataccgcaac aaagatgtcg ctgaactgct caggctgctc 30480
taaaatctgg caggctatga tactgctttt gcttcagctt ctgcttatac ttaccgcagc 30540
ttcagcagca acccctaccc ccaaacccga tgtaagcaag tgtaaattcc accagccatg 30600
gactttcctc caatgctacc atctcaaatc tgagttgccc tcttattaca ttgcgatcat 30660
tggaatcctc accatgctat catgcacatt cttctcattc atgatttacc ccacatttga 30720
ttttgggtgg aatgcaccag attcacttag gctcccacaa tacccagacc cagagccaga 30780
acacattgca ctacagaaca tgcccatacc catattagag tatgaagctg agccacaaca 30840
ccccatgctc cctgctatta gctacttcaa cctaactggt ggagatgact gaccccatgg 30900
aaaactcctc tgccaacgac ctggacatgg acggccgttc atctgagcag cgactggtcc 30960
agatgcgcat tcgccagaag caggaacgcg ccgccagaga gctcaaggat gccattgaaa 31020
ttcacctgtg caagaagggc atcttttgct tggttaagca agcaaagatt tcttatgaaa 31080
tcactgacaa cgaccaccgc ctgtattatg agctcggtcc acagcggcag aaattcacct 31140
gcatggttgg agtcaacccc atagtcatca ctcagcaggc tgcagaaatt aaagggtgca 31200
tccactgttc ctgtgattcc caagaatgcg tccacaccat agtcaagacc ctctgcggcc 31260
ttcgagatct tcttccaatg aactaacccc ttcccccaac ccaataaaac attggtttta 31320
atcataataa aaaatcactt actttaaatc tgaaacagtg tctccgtcca agttttcttg 31380
tagcaccact tcactcccct cttcccagct ctggtactgc aagccccggt gggctgcaaa 31440
ctttctccac accttaaaag ggatgtcaaa ttcctcttgt ccaacaatct tcattgtctc 31500
ttcctagatg tccacaaagc gcgcgcgggt ggaagatgac tttgaccctg tctacccata 31560
cgatgctgag ctggcaccgt ctgtaccctt catcgcccct cccttcgttt cgtcagacgg 31620
atttcaagaa aaacccctgg gagttctgtc cctaagacta gccaacccag tcactactaa 31680
aaatggggaa ctcacactta aactgggaga tggggtgggc atagactcag atggaaacct 31740
cacagcacag acagttacta aagcaacatc cccccttact gtttccaata acgcaattgc 31800
acttaacatg gacaaacctt tttacagtag caatggaaaa ctatccttac aagttacatc 31860
accattaaag atagtcgact ctttaaatac attggctatt ggctatgggc aaggcttagg 31920
actaaacaat agtgctcttg ctgtgcaatt agcatctccc cttacatttg acagcaacag 31980
caaaattaaa ataaatttgg gaagcgggcc attaaaaatt aatgcgaata aactgtcaat 32040
taactgccta aggggtgtat atgtaacaac tgacggaact tcaattgaaa caaatataag 32100
ctgggcaaaa ggaatgaggt ttgaaggtaa tgccatggct gtaaacgttg acagcaccaa 32160
aggtctacaa tttggcacta ccagcacaga atcaggagtc actaacgctt tccctatcca 32220
gttaaagatt ggatctggtc ttagttttga cagcacagga gcacttgtag cttgggataa 32280
ggataatgac aagcttacac tgtggacaac cgctgaccca tcacctaatt gtaccatata 32340
tacagacaag gatgctaaac ttacactttg tcttacaaaa tgtggcagtc aaatactagg 32400
cagtgtttca gtactggctg ttaaagctgg aaccctacag ccaatcagtg aaaaaatagg 32460
tactgctttg gtttcactaa aatttaataa caacggtgta ttgttaagca actccacatt 32520
aagtaatgaa tactggaact acaggaaggg agatgtcaca ccagccgaag cctatactaa 32580
tgctgtgggt tttatgccaa acatcaaggc atatcctaaa aacacaaact ctgcctcaaa 32640
aagccacatt gtaggacaag tgtaccttaa tggagatgaa actaaaccaa tgcatttaat 32700
cattacattt aatgaaacca gtgatgaaac atgcacatat tccataacgt tccaatggaa 32760
atggaacatt ggaacataca ccagcgacac ccttgcaaca agctccttta ccttttctta 32820
cattgcccaa gaataaaaac tgcagacaac aataaagttt aaatgtttta tttaaacagt 32880
tttacacgat tcgagtagtt attttgcctc ccccctccca tttaacagag taaactagtt 32940
tttgcccttg aacagcttta agcaatttta tcccactaga gatagataga tttttagttt 33000
ctacattcca cacagtttca aaacgagcaa atctaggatc agtaagggaa agaaattcat 33060
ctggacaatc ttttagggca gtggcttcac gatcttgctg ctggaaatgt tcatcagtag 33120
tagttgtagg aatcagagtc atctggaaga agaacggtgg gaatcataat ccgaaaatgg 33180
aattgggcga tggtgtctca ttaaaccctg gagcagtctc tgccgatgtc tctcagtgcg 33240
gctgctgctg atgggatctg gatccatagt ctctcgaagc ataatgccaa tagcctttag 33300
catcagcctt cttgtgcgcc ttgcacagca acgcatcctg atctcactca gatcccaaca 33360
gtaagtacag cataacacca caatgttatt tagcaaacca taattaaatg cactccatcc 33420
aaaactcatg gcagggataa cagagccggc atggccatca taccagattc ttacataaat 33480
cagatggcgc cccctaaaga aaacactgcc catgtacata atctctttag gcatgtgaag 33540
gtttacaacc cttcggtacc atggacagcg ctggttaatc atgcatcctt ctataatcct 33600
tctaaaccag atagccaaca cagcccctcc tgccatgcac tgaagggatc cctgattaga 33660
acagtgacaa tgaataaccc acctttccct cccatgaatt acttgggagt aaaaaatatc 33720
tattgtagca cagcacaagc agacattcat gcattttctc attactctaa actcctcagg 33780
tgttaacacc atatcccagg gtacaggaag ctcttgaatg acagtgaaca tagcagaaca 33840
aggtaaacct ctaacataac ttacactatg catagtcaag gtgttacaat ccggaaggta 33900
tggatgttcc tcagtcatag tagctctggt ctcagtctcc tcacatcgtg gtaactggac 33960
cctgtatggg tgacggcgag cggacgacgt cgatcgtccc cgcgacttcg ttgtagtgga 34020
gcgccttccg gacattctcg tactttgagt ggcaaaacct tgctctcgaa cagcacacgt 34080
ctcgtcgcct cctgtccctt ctcttcgcct tttcagtgtg atagttgtaa tacagccatt 34140
cacgaagctc agtcagaaga tcttcagcgt ctgttgtcaa aaacaatcca tccaatctga 34200
ttgctttcaa aacatcacaa acagtcgaat aagccaaacc catccaggca atgcaattat 34260
tttggttatc cacaatggga gggggcggaa gacatggaag aggcatattt aaatttttaa 34320
tccaatcgat cacgcagcac ttcaaaatga agatcgcgaa ggtgacacct ttcaccccca 34380
ctgtgttgat gaaaaataac agccaagtca aaattgatgc ggttttcaag gtgctcgact 34440
gtagcatcaa gcagagcttc cacacgcacg tccacaaata acagaatagc aaaagcggga 34500
ggaggaagta aatcctcaat catcatagta cagtccatca ccatccctaa ataattttca 34560
tccttccagc cttggactat atttttaaac tgctcttgta aatccaaacc acacatgtgg 34620
aaaagttccc aaagagctcc ctcaactacc attcttaagc acaccttcat agtgacaaaa 34680
tatcttgttc ctctgtcacc tgcagcaaat tacaaagtcc aatattagga tctatgccca 34740
gagatctaag ctcatccctc aattccaact gtaaaaaggc ttccagatct gccctaactt 34800
gttcagccag tgggctccct ggaataagcg tgggagaagc caaactgcaa aacagacgca 34860
tgccgccata attaccacca gaaaacacta cgttacagta tgcatgctga ttcattccag 34920
taatttcatc cagtgtattg gatacaaaaa aaggcaagca ctctctcact aattgtatta 34980
tggagacatt atcacacagg taacaattta aaggttgtgg aacaataatg cagtaagtaa 35040
ccacggtgcg ctccaacatg gttagtaatt tttagttctg aaaaacaaaa catacaaaaa 35100
attatatcat actcatttgg cgaactggtg gaaaaatgac cctatctagc acaaggcaag 35160
ccactggatc accaatgcgc ccctcataaa acctgtcatc atgattaaaa agcaacaccg 35220
aaagctcttc cctatgtcct gcatgaatga ttctagctga ggaatataag ccagcgcaat 35280
tagtatctgt taaagaaaaa aaacggccaa catagcctct aggaattagc acacttaatc 35340
ttaaagacat tactgccatc ccccttggat ttaaggtaaa atttacagga gcatagaaaa 35400
tatactgatt tccctcctgc acaggcagca tagcaccagg tccctctaaa aacacacaca 35460
aagcttctgc agccatagct taccgcgcaa accaggcaca gcagtgagct aaaaggacaa 35520
agctctaact cactagccaa cctggcgcac aatatatagt tagtccttac actgacgtaa 35580
ccgaccaaag tctaaaaacc ccgccaaaaa tacacacacg cccaaaaaac gccccgtgag 35640
tcaaaaaaca gtttcacttc ctcgttacac ccaaaacgtc gtcacttccg gattcccacg 35700
gttcgtcact tccggagctc cttgcaaccc cgcccaaaac gtcatcgtcc gcgtcacgcc 35760
gccccgcccc gcgaccgttg accccgggcc aatcaccgca catcccgcaa aattcaaact 35820
cgtctaattt gcatattggc acactgccca tataaggtat attattgatg atg 35873
<210> 6
<211> 1164
<212> DNA
<213> 人工序列
<220>
<223> HAdV-5 E4 orf 6/7
<400> 6
tcacagaacc ctagtattca acctgccacc tccctcccaa cacacagagt acacagtcct 60
ttctccccgg ctggccttaa aaagcatcat atcatgggta acagacatat tcttaggtgt 120
tatattccac acggtttcct gtcgagccaa acgctcatca gtgatattaa taaactcccc 180
gggcagctca cttaagttca tgtcgctgtc cagctgctga gccacaggct gctgtccaac 240
ttgcggttgc ttaacgggcg gcgaaggaga agtccacgcc tacatggggg tagagtcata 300
atcgtgcatc aggatagggc ggtggtgctg cagcagcgcg cgaataaact gctgccgccg 360
ccgctccgtc ctgcaggaat acaacatggc agtggtctcc tcagcgatga ttcgcaccgc 420
ccgcagcata aggcgccttg tcctccgggc acagcagcgc accctgatct cacttaaatc 480
agcacagtaa ctgcagcaca gcaccacaat attgttcaaa atcccacagt gcaaggcgct 540
gtatccaaag ctcatggcgg ggaccacaga acccacgtgg ccatcatacc acaagcgcag 600
gtagattaag tggcgacccc tcataaacac gctggacata aacattacct cttttggcat 660
gttgtaattc accacctccc ggtaccatat aaacctctga ttaaacatgg cgccatccac 720
caccatccta aaccagctgg ccaaaacctg cccgccggct atacactgca gggaaccggg 780
actggaacaa tgacagtgga gagcccagga ctcgtaacca tggatcatca tgctcgtcat 840
gatatcaatg ttggcacaac acaggcacac gtgcatacac ttcctcagga ttacaagctc 900
ctcccgcgtt agaaccatat cccagggaac aacccattcc tgaatcagcg taaatcccac 960
actgcaggga agacctcgca cgtaactcac gttgtgcatt gtcaaagtgt tacattcggg 1020
cagcagcgga tgatcctcca gtatggtagc gcgggtttct gtctcaaaag gaggtagacg 1080
atccctactg tacggagtgc gccgagacaa ccgagatcgt gttggtcgta gtgtcatgcc 1140
aaatggaacg ccggacgtag tcat 1164
<210> 7
<211> 99
<212> DNA
<213> 人工序列
<220>
<223> HAdV-5蛋白IX启动子
<400> 7
ggtactgaaa tgtgtgggcg tggcttaagg gtgggaaaga atatataagg tgggggtctt 60
atgtagtttt gtatctgttt tgcagcagcc gccgccgcc 99
<210> 8
<211> 33040
<212> DNA
<213> 人工序列
<220>
<223> pBLY6.dE1.dE3
<400> 8
catcatcaat aatatacctt atatgggcag tgtgccaata tgcaaattag acgagtttga 60
attttgcggg atgtgcggtg attggcccgg ggtcaacggt cgcggggcgg ggcggcgtga 120
cgcggacgat gacgttttgg ggaggaggag ctatgttgca agtaatcgtg ggaaatgcga 180
cgtaaaacga ggtggagttt aaacacggaa gtagacaatt ttcccgcgct gtttgacagg 240
aaatgatgtg tttttgggcg gatgcaagtg aaaattctcc attttcgcgc gaaaactaaa 300
tgaggaagtg aaattctgag taattctgag gttatcacag ggcggagtat ttaccgaggg 360
ccgagtagac tttgacccat tacgtggagg tttcgattac tctatttttc acctaaattt 420
ccgcgtactg tgtcaaagtc cgtgttttta cgcgatcgcg atggcctgtg tttgagcata 480
atgtactgac caggtgtaac gttcatctgg gtggtcgtag aggaatgttc atgccatacc 540
aatgcaattt taatcatgtg aggatcttga tggagccgca agcgttttcc agagtcagct 600
tgactggaat ctttgacatg tgtgtggaag catggaagat cttaagatat gatgatacca 660
aatccagatg ccgcgcatgc gagtgcgggg gcaggcatgc caggttccaa cctgtatgtg 720
tggaggtgac cgaggagctg agaccagatc atttggtgct gacctgcact ggtgcggagt 780
tcggttccag tggtgaagaa actgattaaa gtgagtagtg ggatgttata aaagtgacca 840
taaggtgatg tgagatggac aaatttggta atttttatgt atttttgtct tgcagccatg 900
agtgggagcg cttcctttga agggggcgtc tttagccctt atctgacggg gcgtctgcct 960
cattgggctg gagtgcgtca gaatgtgatg gggtctacag tggatggaag acctgttcag 1020
cctgctaatt cttctactct gacttatgct actatgactt cctcgccttt ggatgcagct 1080
gcagctgctg ccgcttctgc tgccgccaac actgttcggg ggatggcctt ggagatgggg 1140
tattatggaa ctgtagtggc caacaccact accccaaata accccacagc cttgaatgag 1200
gacaagctgc tagttctcat gtcccagctg gagtctttga cccaacgcct gggcgatcta 1260
gctcagcagg tgtcccagct gaaggagcag actcaagctg caattaccac tgcgagggga 1320
aattaaaaaa attcaaagaa tcaataaata aaccgagact ttgttgattt taaagtgtgt 1380
cattctttat ttaatttttc gcgcgcgata tgccctggac caccggtctc tatcattgag 1440
gacacggtgg atcttttcta gaacccgata gaggtgggat tggatgttga ggtacatggg 1500
cataagacca tctttggggt gtagatagct ccactgcaga gcctcatgct ccggggtggt 1560
gttgtatata acccagtcat agcatgggcg ttgggcatga tgttgcacaa tatctttaag 1620
gaggagacta atggccactg ggagaccctt ggtgtaagtg tttacaaatc tattaagctg 1680
ggacgggtgc atccgaggtg agataatgtg cattttggat tggattttta gattggcaat 1740
gtttccccct agatctctcc tgggattcat gttatgcaag accactagaa cagtgtatcc 1800
ggtgcactta gggaatttgt catgaagttt ggaggggaaa gcatgaaaaa atttagacac 1860
acccttgtgt cctcccaagt tctccatgca ctcatccata ataatggcaa tgggcccatg 1920
ggcggcggca cgggcgaaca cgttcctggg atctgacaca tcatagttgt ggtcttgggt 1980
caggtcatca taagccattt taataaactt ggggcggagg gtgccagatt gggggatgaa 2040
tgttccctcg ggccccggaa catagtttcc ttcacatatt tgcatttccc aggcttttag 2100
ttcagagggg gggatcatgt ccacctgtgg agcgatgaag aagacggtct cgggggcggg 2160
ggtgattaag tgggaggaca gcaagttcct aagcagctgt gacttgccac acccagtggg 2220
accgtagatg acccctataa caggttgcag atggtagttt agggaaagac agctgccgtc 2280
ctctcgcagg aggggggcga cctcgttcat catttccctc acatgcatgt tttcccgcac 2340
aagttccgat aggaggcgct ctccacccag ggaaaggagt tcttgaagag atgagaaatt 2400
tttcaagggt tttaagccat cagccatggg cattttggag agggtttgtt gcaagagttc 2460
aaggcggtcc cagagttcgg tgatgtgttc tatggcatct cgatccagca tacttcctcg 2520
tttctggggt tgggacggct gcgggagtat ggaaccaggc gatgggcgtc cagcgctgcc 2580
agtgtccggt ccttccacgg tcgcagcgtc cgagtcaggg tcgtttccgt cacggtgaag 2640
gggtgcgcgc ctggctgggc gcttgcgagg gtgcgcttca ggctcatcct gctcgtggag 2700
aaccgctgcc gttctgcgcc ctgtgcatcg gccaggtagc aattaaccat gagttcgtag 2760
ttgagcgcct ctgccgcgtg gcctttggcg cgcagcttac ctttggaagt cttctgacag 2820
gtgggacagt agagacactt gagagcatag agttttgggg ctagaaagac cgattctggg 2880
gagtatgcat cggccccaca ggaggcgcag acggtttcgc attccaccag ccatgtaaga 2940
tcgggctcgt tggggtcaaa aacaagtttt ccgccatgtt ttttgatgcg tttcttacct 3000
ttgctttcca tgagttcgtg cccccgttgg gtgacaaaga ggctgtccgt gtccccgtag 3060
actgacttta tgggcctgtc ctcgagcggc gtgccgcggt cctcttcgta gaggaactcg 3120
gaccactctg agacgaaagc acgtgtccag gccagcacaa aggaggctat atgggagggg 3180
tagcgatcgt tgtcaaccaa ggggtctact ttttccaagg tgtgtaaaca catgtcccct 3240
tcttccacat ccaggaaggt gattggcttg taagtgtatg ccacgtgacc tggggtccca 3300
gacggggggg tataaaaggg ggcgggtctc tgctcgtcct cactgtcttc cggatcgctg 3360
tccaggagcg ccagctgttg aggtaggtat tccctctcga aggcgggcat aacctccgca 3420
ctcaggttgt cagtttctag gaacgaggag gatttgatat tgacagtgcc tgccgagatg 3480
cctttcatga gactgtcgtc catttggtca gaaaagacaa tctttttgtt atcaagtttg 3540
gtggcgaagg atccatacag ggcattggaa agcagtttgg caatggagcg catggtttgg 3600
tttttttctt tgtctgcgcg ctctttggcg gctatgttga gttggacata ttcgcgggcc 3660
agacatttcc attgtggaaa tatggtagtt aattcatctg ggacgattct gactttccag 3720
cctctgttat gcagggtaat cagatccaca ctggttgcca cttctcctct aagtggttca 3780
ttagtccagc atagtcgccc cccttttcga gaacagaaag ggggtagggg atctagcatg 3840
agttcgtctg gggggtctgc atctatggtg aaaatcccag gaaggagatc ttcgtcaaaa 3900
tagctgatgg tggcggggtc atccagagac atttgccatt ctcgagcagc cagagcgcgc 3960
tcgtaggggt taaggggagt cccccatggc atgggatggg tgagtgcaga agcatacatg 4020
ccacagatgt catagacata gagcggctct tccagaatcc ctatgtaagt gggataacat 4080
cgcccccctc tgatgctggc tcgcacataa tcatagagtt catgtgaggg cgctagaaga 4140
cccgagccca ggttggtgcg gttgggtttt tctgctctgt agaggatctg gcgaaagatg 4200
gcatgggagt ttgatgagat ggtgggtctt tggaagatgt tgaaatgggc atgaggcagt 4260
cccacagagt cccttatgaa gtgagcatag gagtcttgca gtttggccac cagctcggcg 4320
gtgaccagca catccaaagc acagtagtcg agggtctctt tgatgatgtc atagttaggt 4380
tcccctttct tttcccacag ctcgcggttg agaaggtatt cttcgcgatc cttccagtac 4440
tcttcgaggg ggaacccgtc cttgtctgaa cggtaagaac ccagcatgta aaattgattg 4500
acagctttgt aggcacaaca ccccttctcc acggggagtg agtatgcttg cgcggctttg 4560
cgcagagagg tgtgagtaag ggcgaaagtg tccctgacca tgactttgag gaactgatgc 4620
ttaaagtcta tgtcatcgca ggccccctgc tcccacagtt ggaagtccac tcgctttttg 4680
taggcgggat tgggcaaagc gaaagtaaca tcgttgaata ggatctttcc agccctgggc 4740
atgaagttgc gagtaatgcg aaaaggctga ggcacttctg ccctgttgtt gataacttgg 4800
gcagccaaga cgatctcgtc aaagccgttg atgttgtgac ccacaatgta aagttctacg 4860
aagcgtgggc gtcccttgat gtggggcagt tttttaagct cttcgtaggt caagtcgtca 4920
gggtcagcga ttccatattg ctccaaagcc cagtcaggca ggtgaggatt agcatgaagg 4980
aaagaggtcc aaagatccac ggccagagct gtttgtaagc ggtctctgta ctgacggaaa 5040
tgtcggccta ccgccatttt ttcaggagta acacagtaaa aggtgcgcgg gtccttttcc 5100
cagcgatccc attgaagttg caaggctagg tcgtgggcga ggttgacgag ctgttcgtcc 5160
cccgaaagtt tcatgaccag catgaaaggg acaagctgct tgccaaagga ccccatccag 5220
gtgtaggttt ccacatcgta ggtgaggaag agcctttctg tgcgaggatg agaaccgatc 5280
gggaagaact ggatttcctg ccaccagttg gaggaatggc tgttgatatg atggaagtag 5340
aactccctac ggcgcgccga gcattcgtgc ttgtgcttgt acagacggcc acagtactcg 5400
cagcgctgca cgggatgcac ctgatgaatg agctgtacct ggcttccttt gacaagaaat 5460
ttcagtggga agttgaggcg tggcgtctgc atctcgtgtt gtattacgtc ctggctattg 5520
gtctggccat cttctgtctc gatggtggtc atgctgacga gcccgcgcgg gaggcaggtc 5580
cagacctccg cgcggacggg tctgagagcg aggacgagag cgcgcaggcc ggaactgtcc 5640
agggtcctga gacgctgcgg agtcaggtca gtagggagag tacataggtt tacttgcata 5700
agtttttcca gggcatgtgg gaggtcaaga tgatatttga tttctactgg cgagttggtg 5760
gagacatcga tggcttgcag ggtcccgtgc ccctggggtg ctaccaccgt cccttttttt 5820
ttcttgatcg ggggcggtgt tgcttcttgc atggtaaggt cgtcttctag aagcggcggc 5880
gaggtcgcgc gccgggtggc agtggcggtt ctggacctgg aggtagaggc ggtagaggta 5940
cgtcggcgcc gcgcgcgggt aggttctggt actgcgccct gagaagactt gcgtgagcga 6000
cgacgcggcg gttgacgtcc tggatctgac gcctctgggt gaatgctacc ggacccgtga 6060
gcttgaacct gaaagagagt tcaacagaat caatttcggt atcgttgacg gctgcctgcc 6120
gcaggatttc ttgtacgtcg cccgagttgt cttggtaggc gatctcggcc atgaactgct 6180
cgatctcttc ttcttggaga tctccgcggc ccgctcgttc tacggtggca gcaaggtcgt 6240
tggagatgcg ccccatgagc tgtgagaatg cattcatgcc cgcctcgttc cagacgcgac 6300
tgtagaccac ggctccctcg ggatctctgg cgcgcatgac cacttgggcg aggtttagtt 6360
ccacgtgtct ggtgaagacc gcatagttgc agagacgctg gaagaggtag ttgagcgtgg 6420
tggcgatgtg ctcggtgaca aagaaataca tgatccagcg acgaagcggc atctcgctga 6480
tatcgcccag ggcttccaac cgttccatgg cttcgtaaaa gtccacggcg aagttgaaaa 6540
actgggagtt gcgagcggac acggtcaact cctcctccag aagacggatg agctcggcga 6600
tggtggcgcg cacttcgcgc tcaaaggctc ccgggatctc ttcctcctct tcttcttcca 6660
actcttcctc cactaacatc tcttctactt cctcctcagg cggcgggggt ggaggagggg 6720
gcgcgcggcg acgccggcga cgcacgggca gacgatcgat gaagcgttcg atcacttctc 6780
cgcggcggcg acgcatggtc tcggtgacgg cgcgcccgtc ctccctgggt cgcagagtga 6840
agacgccgcc gcgcagctcc ctgaaatggt gactgggagg gtccccgttt ggtagggaca 6900
gggcactgat gatgcatctt attaattgcc ctgtagggac tccgcgcaag gacctgagcg 6960
tctcgagatc cacgggatct gaaaatctct gaacgaaggc ttcgagccag tcgcagtcgc 7020
aaggtaggct gagcactgtt tcttcggggc gggctgctga gctagagggt tgtacgatgc 7080
tgctggtgat gaagttaaaa taggcagttc tgagacggcg gatggtggcg aggagcacca 7140
ggtctttggg tccggcttgc tggatgcgca ggcggtcggc cattccccat gcattatctt 7200
ggcacctggc cagatcttta tagtagtctt gcatgagtcg ctccacgggc acttcttctt 7260
cgcccgctct gccgtgcatg cgtgtgagtc cgtaccctct ctgtggttgg acgagcgcca 7320
ggtcggcaac gaccctttcg gctagaatgg cttgctgcac ctgggtgagg gtgttctgga 7380
aatcatcaaa gtccacaaag cggtggtagg cccccgtgtt gatggtgtag gagcagttgg 7440
acatgaccga ccagttgact gtctggtgtc ctggtcgtac gagttccgtg tacctgagcc 7500
gcgagtatgc gcgggagtcg aagatgtaat cgttgcaggt tcgcaccagg tactggtagc 7560
cgatgaggaa gtgaggcggc ggctggcggt agagaggcca tcgttcggtg gcgggcgcgc 7620
cgggcgctag gtcttctagc atgagacggt ggtatccgta gacgtacctg gacatccagg 7680
taataccggc ggcggtggtg gaggcgcgcg gaaactctcg cacgcggttc cagatgttgc 7740
gcagcggcat gaagtagttc atggtgggca cggtctggcc cgtgaggcgc gcgcagtcat 7800
tgatgctcta gatacgggca aaaacgaaag cgttgagcgg ttcccttccg tggcctggag 7860
gaacgcgaac gggttaggtc gcagcgtacc ctggttcgag actaaagaaa gcgagcaact 7920
cgaaccggca gagtcgcggc taacgggtat tggcaatccc gtctcgaccc aagccagcaa 7980
atccaggata cggatggggg ccccttttgt ttttcagggc atgagtcacc ggttaaggtt 8040
tacaacggct gtttcatgcc tttagaagtg gctcgcgccc gtagtctgga gaatcaatcg 8100
ccagggttgc gttgcggcgt gccccggttc gagcctgcag cttgagtcgg ccggtgaccg 8160
cggcaaacga gggcgtggcg gccccgtcgt ttctaagacc ttgctagccg acctctccag 8220
tttacgggaa cgagccccct tttatttttt ttgtttttgc cagatgcatc ccgtactgcg 8280
gcagatgcgc ccacagcccc cacagcagca gcagcaggct ggcctacctt ctctacctca 8340
gccgctacct gcaactaccg cggtggccgc tgtaagcggg gccggacagc aggcggctcc 8400
tcaatatgaa ttggacttgg aagagggcga gggattggca agattggggg cgccctcgcc 8460
cgagcgccac ccgcgggtgc agatgaaaaa ggacgttcgc gaatcttacg tgcccaagca 8520
gaatctgttc agagacagaa gcggcgagga gcccgaggag atgcgcgcgt cccgttttaa 8580
cgcgggtcgc gagctgcgac aaggactgga tcgaaaacgg gtgttgaggg atgatgattt 8640
tgaggtggat gaaatgacag ggatcagccc cgctcgcgct cacgtggctg cagctaatct 8700
ggtgacagct tatgagcaga ccgtgaagga ggaaagcaac ttccagaaat cattcaataa 8760
ccacgtgcgc accctgatcg cacgcgagga ggtgaccctg ggcctgatgc acctgtggga 8820
tctgctggaa gccatagtgc agaaccccac tagcaaaccc ctgactgctc aactgtttct 8880
ggtggtgcag cacagcaggg ataatgaggc attcagagag gcgctgctga atatcactga 8940
acctgagggg agatggctgc tggatctggt gaatatcctg cagagcattg tagtgcagga 9000
acgcagcttg cctttgtccg agaaggtggc ggcgatcaat tactctgtgc tgagtctggg 9060
caaatactat gccaggaaga tctacaaaac cccttacgtg cccatagaca aggaagtgaa 9120
aatagatggg ttttacatgc gcatgaccct gaaagtgcta accctgagcg atgacttggg 9180
agtgtaccgc aacgacagga tgcaccgcgc ggtgagcgcc agcaggaggc gcgagctgag 9240
cgacaaagaa ttaatgcaca gcttgcaacg agccctgacg ggagccggga cggaggggga 9300
gaactacttt gacatgggtg cagacttgca ttggctgcct agtcgcaggg cattggaagc 9360
ggcaggcgat gggccctatg tagaggaagt agtagacgag gacgatgagg agggcgagta 9420
cctggaagac tgatggcgcg acccgtattt ttgctagatg gaacaggcgc cggaccctgc 9480
gatgcgggcg gcgctgcaga gccagccgtc cggcattaat tcctcggacg attggaccca 9540
ggccatgcaa cgcatcatgg cgctgacgac ccgcaacccc gaagccttta gacagcagcc 9600
tcaggccaac cgcctttcgg ccatcctgga ggccgtggtg ccctctcgct ccaaccccac 9660
ccacgagaag gttctggcca tcgtgaatgc cctggtggag aacaaggcca tccgctccga 9720
tgaagccggg ctggtataca acgccttgct cgagcgcgtg gctcgctaca acagcagcaa 9780
tgtccagact aacctggaca ggatggtgac cgacgtgcgc gaggccgtgt cccagcgcga 9840
acggttccat cgcgagtcta acctgggttc catggtagcg ctgaacgctt tcctcagttc 9900
ccagcctgcc aatgtgcccc ggggacagga agactatacc aactttatta gcgccctgag 9960
actcatggta gccgaggttc ctcagagcga ggtgtaccag tccggtccag actacttttt 10020
ccagacaagc aggaacggta tgcagacagt gaacttaagc caggctttca agaacctgca 10080
agggctgtgg ggagtccaag ctccagtggg cgacagggcg accgtgtcga gcctgttgac 10140
tccaaattcc cgtttgctgc tgctgctggt gtcccccttc actgacagcg gcagcataaa 10200
cagaaactcc tacttgggct acctgataaa cttgtatcgc gaagctatag gtcaggccca 10260
cgtggacgaa cagacctatc aggagatcac taatgtgagt cgcgctctgg gccaggacga 10320
ccctggaaac ctggaagcta ctctaaactt tctgctgacc aaccgctcgc aaaaaatccc 10380
tcctcagtat acattaactg cggaggagga acggatcttg agatacgtgc agcagagcgt 10440
gggtctgttc ctgatgcaag agggtgcgac ccctagcgcc gcgcttgata tgacagcgcg 10500
caacatggag cccagcatgt atgccagcaa cagaccattc attaataaat tgatggatta 10560
cttccatcgc gcggccgcta tgaactctga ttacttcacc aatgctattc tgaaccccca 10620
ttggctgcct ccgcctggtt tttatactgg cgagtatgac atgcctgacc ccaacgatgg 10680
gttcttgtgg gacgatgtgg acagcgtggc gttctcgcct accgctcctc gtactttttg 10740
gaagaaggaa ggtagtgaca gaagaccctc ctccgtgctg tcaggacgtg agggtgctgc 10800
cgcggcggtc cccgatgctg caagcccctt tcccagtctg ccattttcac taaacagcgt 10860
gcgcagtagc gagctgggga gaataacccg ccctcgcttg ctgggcgagg acgagtattt 10920
gaatgactcc ctactgagac ccgagcggga aaagaacttc cctaataatg ggattgaaag 10980
cctggtggat aagatgagca gatggaagac ctatgcccag gagcacagag atgagcctag 11040
aatcttgggt cctacagtag gcacccgcag acgccagcgc catgatagac agcggggtct 11100
ggtgtgggac gatgaggatt ctgcagatga cagcagcgtg ttggacttgg gcgggagggg 11160
aggtgtgggc aacccgttcg cacacttgcg tccccgtatt ggacgcatga tgtaaaagtg 11220
aaaataaaaa aggaactcac caaggccatg gcgaccagcg tgcgttcgtt ctttctgttg 11280
ttgtatctag tatgatgagg cgcaccgtgc taggcggatc ggtggcgtat ccggagggtc 11340
ctcctccttc gtacgaaagc gtgatgcagc aggtggcggc ggcggcgatg caacccccct 11400
tggaggctcc ttacgtgccc ccgcggtacc tggcacctac cgaggggaga aacagcattc 11460
gttattcgga actcacaccc ttgtatgaca ccacccggtt gtacctggtg gacaacaaat 11520
cggcggacat tgcctcgttg aactatcaga acgaccacag caacttcttg acaacggtgg 11580
tgcagaacaa tgactttacc cccacggagg ccagcaccca gaccatcaac tttgacgagc 11640
gctcccggtg gggcggtcag ctgaagacca tcatgcacac caacatgccc aacgtgaacg 11700
agttcatgtt tagcaacaag ttcagggcta gggtgatggt gtccagaacc acacctaaag 11760
aggtgacagt cacaacagac tatgatggta gtcaggacat cttggaatac gagtgggttg 11820
actttgagtt accagaaggc aacttctctg ccaccatgac catagacctg atgaataatg 11880
caattgttga taattaccta aaagtgggta gacagaatgg ggtactggag agtgacatag 11940
gtgttaagtt tgacactagg aactttaggc ttggttggga cccagtgaca gagttggtca 12000
tgcctggggt ctacaccaat gaagctttcc atcctgacat agtcctacta cctggctgcg 12060
gagtggactt cactgagagc cgcctcagta atctgctagg cattagaaag aaacagccat 12120
tccaggaagg gttccagatc atgtatgagg atctggaggg tggtaacatc cccgccctgc 12180
ttgatgtaaa tgcatatgag aagagcaagg aagataatac aaccaccaca aatgaagctg 12240
tggccgcggc ttcatctact gaagccaaag ctgtggtaga tgcttccact tcaacagaaa 12300
acaccactga tgaaaaagtc accaggggag atacatttgc cacccctgaa caagagaagg 12360
cagctgaggc agagtctgat attatgcttc tgtccaccga tgaaaacgaa actaaaaaac 12420
aactggttat tcgagcggtg accaaggata gtaaggacag gagttataat gtattgtcag 12480
atggaaagaa cacagcttac cgtagttggt acctggcata caattatggc gaccgtgaga 12540
aaggggtgcg ttcttggaca ctgcttacca cctcggatgt cacctgcggc gtggagcaag 12600
tctattggtc gctaccagat atgatgcaag atccagtcac ctttcgctcc acacgccaag 12660
ttagcaacta cccagtggtg ggcgcagagc tgctcccagt gcattccaga agcttctaca 12720
acgagcaagc cgtctactcg caacagctcc gccagtacac ctcgctcacg cacgtcttca 12780
accgcttccc cgagaatcag atcctcgtcc gcccgcccgc gccaaccatt accaccgtca 12840
gtgaaaacgt tcctgctctc acagatcacg ggaccctgcc gctgcgcagc agtatccggg 12900
gagtccagcg cgtgaccgtt actgacgcca gacgccgcac ctgtccatac gtatacaagg 12960
ccctgggcat agtcgcgccg cgcgtccttt caagccgcac tttctaaaaa aatgtccatt 13020
ctcatctcgc ccagtaataa caccggttgg ggcctgcgca cacctagcaa gatgtatgga 13080
ggcgctcgca gacgctccac tcagcaccct gtgcgcgtgc gcgggcattt ccgcgctccc 13140
tggggcgccc tcaagggacg ctctcgtact aggaccaccg ttgacgatgt gatcgaccag 13200
gtggtcgccg atgcacgtaa ctataccccc gcagccgcac ctgcatccac cgtggatgcg 13260
gtcattgaca gcgtggtagc cgatgcgcgc gcctatgctc gcgccaagag caggaggcgg 13320
cgtattgcca ggcgtcaccg agctactcca gccatgcgag ctgcaagagc tttattgcgg 13380
agagccagac gtgtggggcg aagagccatg cgtagagcgg ccagacgcgc ggcttcaggt 13440
gccagcgcag gcagggtccg caggcgcgcg gctacggcgg cagcggcggc catcgctagc 13500
atgaccaaac cacgaagagg caatgtgtat tgggtgcgcg acgccgccac cggccagcgc 13560
gtgcccgtgc gcacacgccc ccctcgcact tagaagatac tgagcagtct ccgatgttgt 13620
gtcccagcgg cgagatgtcc aagcgcaaat tcaaggaaga gatgctccag gtcatcgcgc 13680
ctgagatcta cggtcctgcg gtgaaggatg aaaaaaagcc ccgcaagatc aagcgggtca 13740
aaaaggacaa aaaggaagaa gatggtgatg atgggctggt ggagtttgtg cgcgagtttg 13800
ccccaaggag gcgcgtgcag tggcgcgggc gcaaagtgtg gccggtgttg agaccgggga 13860
ccacagtggt ctttacgcca ggcgagcgct ccagcaccgt ttccaaacgc tcttatgatg 13920
aggtgtacgg ggacgatgat attctcgagc aggcggctga tcgccttggc gagtttgcat 13980
atggcaaacg cagccgctcg ggagccaagg aagaggcatt gaccatcccc ttggatcatg 14040
gaaatcccac cccaagcctc aaacccgtga ccctgcaaca agtgctgccc acgccgccac 14100
gcaagggcat caagcgcgag ggcgaggatc tgtatcccac catgcagctg atggtgccca 14160
agcgccagaa gctggaagac gtgctggaga aaatgaaagt ggatcctgaa atccagcctg 14220
aagtcaaagt gaggccaatc aagcaggtgg cgcccggttt gggggtacaa accgtggata 14280
tcaagatccc caccgagtcc atggaaattc aaaccgaacc catgaagccc acctccagca 14340
ccattgaggt gcagacggat ccttggatgc ccgcgcctgc tcctgttacc actactactc 14400
gaagacctag aagaaagtat ggttcagcca acctgataat gccaaactat gctctgcatc 14460
catcaatcat acccactcct ggctaccgcg gcactcgcta ctaccgcagt cacagcaccc 14520
gccgacgtaa agcacctgcc acccgccgcc gtcgccgccg ccgtgccact agcaaactta 14580
ccccctcggc tatggtgcgg agagtgtacc gtgatgggcg cgcagctcct ctgacactgc 14640
cgcgcgcgcg ctaccatcct agcattgcca tttaacaact ctgcctcctt gcagatatgg 14700
ccctcacttg ccgccttcgt attcctattg ctggctaccg cggaagaaag tcgcgccgta 14760
gaagagcagg gttgtctggg agcgggatgc gtcgccaccg gcggcggcgc gccatcagca 14820
aacggttggg gggtggattt cttcccgctt tgattcccat catcgccgcg gcgatcggcg 14880
cgataccagg catagcttcc gtggcggtgc aggcctcgca gcgccactga cattggaaaa 14940
agatatctta taaataaaaa tagaatggac tctgacgctc ctggtcctgt gatatgtttt 15000
tgtagacgag atggaagaca tcaatttttc atccctggct ccgcgacacg gcacgcggcc 15060
gtatatgggc acctggagcg acatcggcaa cagccaactg aacgggggag ccttcaattg 15120
gagcagtcta tggagcgggc ttaaaaattt tgggtccact ataaagactt atgggaacaa 15180
agcttggaac agcagcacag ggcatgcgct gagacaaaag cttaaagatc agaatttcca 15240
acagaaggtg gtcgatggta tcgcctctgg aatcaatggg gtggtagatc tggccaacca 15300
ggccgtgcag aaacagatta acagtcgcct ggacccggct cccccagctc ctattcatga 15360
gttaatgcaa gtggaggaag agctcccttc attggaaaag cggggcgata agcgacctcg 15420
tccagatatg gaggaaacgc tgctgaccaa ggtggatgag ccgccctcct atgaagaggc 15480
tgtaaaactg ggaatgccca ctacaaagcc cattatgcct ctggccactg gagtgatgaa 15540
gccatctcag tctaaacctg cagttgctgc tacattggac ttgcccgctc ccgtggccac 15600
ccccaaacct gtcgccgccc cgaagcccac cgccgtgcaa cccgtggccg tggccagacc 15660
gcgtcccggt ggtcggccga atgcaaactg gcagagcact ctgaacagca tcgtgggttt 15720
gggagtgcac agtgtgaagc gccgtcgctg ctattgatta aatatggagt agcgcttaac 15780
ttgcttgtct gtgtgtgtat atgtcgatgc cgcccgccgt gctacagcaa agagagaagg 15840
agaagaggcg ccgctgagtt cctttcaaga tggccacccc atcgatgctg ccccagtggg 15900
cgtacatgca catcgccgga caggacgctt cggagtacct gagtccgggt ctggtgcagt 15960
tcgcccgcgc cacagatacc tacttcaatc tggggaacaa gtttaggaac cctaccgtgg 16020
ctcccaccca cgatgtgacc accgaccgta gccagcgcct gacgctgcgc tttgtgcccg 16080
ttgaccggga ggacaatacc tactcctaca aagtcagata caccctggct gtgggagaca 16140
acagggtgtt ggatatggcc agcacctact ttgacatcag gggcgtgttg gacagaggac 16200
ctagcttcaa accatactct ggcactgcct acaactccct ggctccaaaa ggagctccaa 16260
actccagtca gtggcaacaa aaggaaaaca atggtcaagg tgatgcaaag actcacacct 16320
atggtgtagc tgccactgga ggtattgaca ttgacaaaaa tggtcttcaa attggaatcg 16380
atgaaactaa agaagataat aacgaaattt atgcagacaa aacattccaa cctgaacctc 16440
aaattggaga agaaaactgg caagatagcg aaaactatta tggaggcagg gctcttaaac 16500
cggaaaccaa gatgaagcct tgctatggtt ccttcgctag accaactaat gcaaagggag 16560
gtcaagccaa aattaaacca gctcaagagg gtcaacagtc tatagattat gacatagacc 16620
tggctttctt tgatattcca agcactggcg gaggcaatgg cacaaatgta aatgacaagc 16680
cagatatggt tatgtatact gaaaatgtaa atctggaaac tccagacact catcttgttt 16740
acaagccagg aacttcagat gacagttccg aggccaattt aactcagcaa gccatggcta 16800
acagacccaa ctatattggg tttagagata actttattgg cgtcatgtac tacaacagca 16860
ctggcaacat gggagtgctt gctggtcaag catcccagct aaatgctgtg gtggacctgc 16920
aagacagaaa caccgagctg tcttatcagc tattacttga ctctctgggc gacagaacca 16980
ggtattttag tatgtggaat caggcggtgg acagctatga tcctgatgtg cgcattattg 17040
aaaaccatgg tgtggaagat gaattgccaa actattgctt cccattggac ggagctggca 17100
ctaatgctgt ttaccaagga gttaagacaa aagaggataa taatggcgaa tgggaaacag 17160
acacaaatgt tgcatcgcag aatcagatat gcaagggcaa catatatgct atggagatca 17220
acctgcaagc caacctgtgg aaaagtttcc tttactccaa cgtggctctg tacctaccag 17280
actcctacaa gtacactcca tccaacgtga cactccctac caacactaac acctatgact 17340
acatgaatgg cagggtggtg tctccatccc tggtggatgc ctacattaac attggcgcca 17400
ggtggtctct ggatgccatg gacaatgtca accctttcaa ccaccaccgc aatgccggcc 17460
tgcgctaccg gtccatgctt ctgggcaacg gccgatacgt gcccttccac atccaagtgc 17520
cccagaaatt cttcgctatc aagaacctgc tgcttctccc aggctcatac acctacgagt 17580
ggaacttccg caaggatgtc aacatgatcc tgcagagttc ccttggcaat gacctcagaa 17640
ccgatggggc caccatccag tacaccagca tcaatctcta tgccaccttc ttccccatgg 17700
ctcacaacac tgcctccacc ctggaagcca tgctgcgcaa tgacaccaat gaccagtcct 17760
tcaatgacta cctctcagct gccaacatgc tttaccccat ccctgccaat gccaccaacg 17820
tgcccatctc catcccatct cgtaactggg ctgccttcag gggctggtct ttcacccgcc 17880
tcaagaccaa ggagacccca tctctgggat cagggttcga tccctacttc gtctactcag 17940
gctccattcc atacctggat ggaactttct accttaacca cactttcaag aaagtctcca 18000
tcatgtttga ctcttctgtc agctggccag gcaatgacag gctgctgact cccaatgagt 18060
tcgaaatcaa gcgcactgtt gatggggaag ggtacaatgt ggcacaatgc aacatgacca 18120
aagactggtt cctggttcag atgctctccc actacaacat tggctaccag ggcttctaca 18180
tcccagaagg atacaaggac cgcatgtact ccttcttcag aaacttccag cccatgagcc 18240
gccaggtggt cgatcaggtc aactacaaag actacatggc agtcaccctt gcctatcagc 18300
acaacaactc tggctttgtg ggctacctcg cgcccaccat gcgacagggc caaccctacc 18360
ctgctaacta cccatacccg ctcattggca agactgcagt caacagtgtc acccagaaaa 18420
agttcctctg cgacagggtc atgtggcgca tccccttctc cagcaacttc atgtccatgg 18480
gggcccttac cgacctgggg caaaacatgc tttatgccaa ctccgcccac gcgctagaca 18540
tgaatttcga agtagacccc atggatgagt ccacccttct ctatgttgtc ttcgaagtct 18600
tcgacgtggt cagagtgcac cagccccacc gcggcgtcat cgaagctgtc tacctgcgca 18660
cccccttctc agctggtaac gccaccacat aagcgccttg cttcttgcaa gtggctgcag 18720
cagcatggcc tgtggatcct ccactggatc caatgagcaa gagctcaggg ccatcgccat 18780
agacctgggc tgtggaccct atttcctggg aacctttgac aagcggtttc caggcttcat 18840
ggctcctgac aagctcgcct gtgccattgt caacacggca gggcgcgaga ctggtggtga 18900
gcactggctg gcttttggat ggaacccccg ctccaatacc tgctatctct ttgacccgtt 18960
tgggttttca gacgagcgcc tcaagcagat ctatcaattc gagtacgagg ggctcctgcg 19020
ccgcagtgcc ctggctacta aggaccgatg catcactctg gaaaagtcta cccagaccgt 19080
gcagggtccg cgctcggctg cctgcgggct cttctgctgc atgttcctcc atgcttttgt 19140
gcactggccc gaccgcccca tggacaacaa ccccaccatg aatttgctga cgggggtacc 19200
caacaacatg ctccaatcgc cccaagtaga gcccaccctg cgccacaacc aggaggcact 19260
ctatcgcttc ctgaactccc actcatctta ctttcgttct aaccgcgcgc gcattgagaa 19320
ggccactgcc ttcgatcgaa tgaataataa catgtaaacc aaattgtgtg tggctcaaat 19380
aaacagcact ttattgttta catgcactga ggctctggga tgatcatttt ttaaaaatcg 19440
aaggggttct ggcgggaatc agcatggcca gatggcaggg acacgttgcg gaactggaac 19500
ttgttctgcc acttgaactc gggaatcacc agcctgggaa ctggaatctc tggaaaggta 19560
tcttgccata gctttctggt cagttgcaga gcgccaagca ggtcaggagc agatatcttg 19620
aaatcacagt tggggccaga attctgggcg cgggagttgc ggtacactgg gttgcagcac 19680
tggaacacca taagggcagg gtgtctcacg ctcgccagca cggtctcgtc actgatgcaa 19740
gacacatcca ggtcttcagc attggccatt ccaaaggggg tcatcttgca ggtctgtctg 19800
cccatcacgg gagcgcagcc aggtttgtgg ttgcaatcac aatgaagggg gatcagcatc 19860
atcttggcct ggtcgggggt aatccctggg taaacagcct tcatgaaggc ttcatactgc 19920
ttgaaagctt cctgggcttt ggttccctcg gtgtagaaca ctccacaaga cttgctggaa 19980
aactgattag tagcgcagtt ggcatcattc acacagcagc gggcgtcgtt attagccagc 20040
tggaccacat tcctgcccca gcggttctgg gtgatcttgg ctcgatctgg gttctccttc 20100
aacgcgcgct ggccgttctc gctcgccaca tccatctcaa tgacatgttc cttctggatc 20160
atgatgttgc catgcaggca tctaatcttg ccttcataat cagtgcagcc atgaggccac 20220
agcgcgcacc cggtgcactc ccaattgtta tgggggatct gggaatggct atgaaccagc 20280
ccttgcagga atcttcccat catcacagcc agggtcttta tgctggtaaa ggtcagcggg 20340
ataccgcggt gctcctcgtt cacatactgc tggcagatgc gtctgtagtg ctcggcctgc 20400
tcgggcatca gcttgaaaga ggttttcaac tcattatcca gcctgtatct ctccatcatg 20460
atggacatta cttccatgcc cttctcccag gcagaaacaa tagggagact caggggattc 20520
ttgacagtag agacaacctt acttaagggg tcatcactgc caatcttttc gatgcttctc 20580
ttgccatcct tctcggtgat gcgcaccggc gggtagctga atcccacagc caccaactga 20640
gcctcttccc tttcgtcttc gctgtcttga ctgatgtctt gcagaggaac atgtttggtt 20700
ttcctgggtt tcttcttggg cggcagctct ggaggactct ggctccgttc cggagacccc 20760
atggatgagc gagagttgtc gctcaccact tggatctggc tgcctgtaga agaactggac 20820
cccacgcggc ggtaggtgtt cctcttggta ggcagaggtg gaggcgacgg gctccggtcc 20880
ggtctgggtg gcggatggct ggcggagccc cttccgcgtt cgggggtgcg ctccagatgg 20940
cggtcgtctg actgacctcc gcggctggcc attgtgttct cctaggtaga gaaacaagac 21000
atggagactc agccatcgct gccatcgcca tccaccacca caagcaccgc cgaggaggag 21060
gagtgtttaa ccaccccacc atgcagcccc gctaccacca ccagcaccct tgaaagcgag 21120
gtcgacacgg tcgtggagga tttacaggct atggaagata ttgaggcagc tgtcgagcaa 21180
gaccccggct atgtgacacc ggcggagcat gatgaggatc tagcgcgctt tctcgacggt 21240
gtggagaaag cgaaacaaga tgaggacgag gaagaggcag aagcacaacc atcggtggcc 21300
gactacctca ccggcctagg gctagaagac gtgctgctta agcatcttgc aaggcagaca 21360
gtcatagtca aagacgccct gctagagcgc tccgaggtgc cactcagtgt ggaagacctc 21420
agtcgcgcct atgagctaaa cctcttctcg cctcgcaagc cccccaagcg tcagcccaac 21480
gggacctgtg agcccaatcc gcgcctcaac ttctatccag ccttcactgt gcccgaagta 21540
ctagctacct accacatctt tttcaagaac caaaagatcc ccatctcctg ccgcgccaat 21600
cgcacccgcg cagatgccct actcaacttg gggcccggcg ctcgcatacc tgatatcgct 21660
tccttggaag aggttcctaa gatctttgag ggtctgggca atgaggaaac tcgggcagca 21720
aacgctctgc aaagagaaac agatgatggt gaacaccaca gcgctctggt ggagctccag 21780
ggcgacaacg ctcgtcttgc agtcctcaaa cgcagcatcg aggtcaccca tttcgcctac 21840
cccgcactta atctcccacc caaagtcatg agctcggtca tggacacgtt gctcatgaag 21900
cgcgcgagcc ccatctccga ggatcagaac atgcaggacc ccgatgcctc agatgaaggc 21960
aagcctgtag tcagcgacga gcaactggct cgctggctag gctctgactc cccccagtct 22020
ttggaggagc ggcgcaagct tatgatggca gtggtcctga tcacagcgga gctggagtgt 22080
ctccgccgct tcttcactga cccagagacc ctgcgcaagc ttgaggagaa cctgcattac 22140
acattcagtc atgggttcgt gcgccaggcg tgcaagatct ccaacgttca actcaccaac 22200
ctggtctcct acctgggcat cttgcatgaa aaccggctgg ggcagaacgt gctccacacc 22260
accctgaagg gggaggcccg ccgcgactat atccgcgact gtatctacct ctacctatgc 22320
tacacctggc aaagcgggat gggtgtgtgg caacagtgct tggaagagca aaatctaaaa 22380
gagctggaaa agctgcttca gaaatctctt aaatctctgt ggaccgggtt cgatgagcgg 22440
accaccgctt cggacatggc cgatattatc ttccccgagc ggctcagaca cactctgcgc 22500
gacgggctgc ctgactttgc cagccagagc atgctacaaa actttaggtc attcatcttg 22560
gaacgctccg ggatcctgcc cgccacttgc tgcgcactgc cctccgattt tgtgcccatc 22620
acctaccggg agtgcccccc gccgctatgg agccactgct acctgttccg cctggccaac 22680
tacttggcct accactctga tgtgatagaa gatgttagtg gcgaagggct cctggagtgc 22740
cactgccgct gcaacctctg caccccccac cgctccctcg cctgcaatcc ccagctgctg 22800
agcgaaaccc agatcatcgg caccttcgag ttgcaaggtc ccagcggcga aggcgagggg 22860
tcctctccgg ggcaaagtct gaaactgact ccggggctat ggacctccgc ttaccttcgc 22920
aagttcgccc ccaaagacta ccacccctat gagatcaggt tttatgaaga ccaatcacag 22980
ccccccaagg ccgaactgac ggcctgcgtc atcacccagg gggcaatctt ggcccaattg 23040
caagccatcc aaaaatcccg ccaagaattt ttgctgaaaa agggacacgg gatctatcta 23100
gacccccaga ccggtgagga gctgaataca cgcttccctc aggatgcccc gaggaggcaa 23160
gagaatgaaa gttcagatgc cgcccgagga ggagctggaa gactgggaca gtcaggcaga 23220
ggaggaagac tgggacagcc aggcagaaga ggaggacagc ctggaggagg acagtctgga 23280
ggaaggcgag gagcccaagg aagaggcagc cgccgccaga ccatcgtcct cggcggtgga 23340
gacaagcaag gtcccagaca gcacggctac cacctccgct ccagctcaag gggccgctcg 23400
gcgacccaac agtagatggg acgagacggg tcgcttccag aaccccacca ccgtcaagac 23460
cggtaagcag gagcggcagg gatacaagtc ctggcggggg cataaaagtg ccatcatcgc 23520
ttgcttgcag gagtgtgggg gcaatatatc ctttgccaga cgctacctgc tattccatca 23580
cggggtgaat ttcccccgca acatcttgca ttactaccgt cacctccaca gcccctacta 23640
ccagcagcaa gagacagcag aggaaaccag cggcaactcc gagagttaga aaaccagcag 23700
ctaaaaaatc cacagcggcg gcagcaggtg caggcggact gaggatcacc gcgaacgagc 23760
cagctcagac cagggagttg aggaatcgga tctttcccac cctctatgcc atattccaac 23820
aaagtcgggg tcaggaacaa gaactgaaag taaaaaacag atctcttcgc tcgctcaccc 23880
gcagttgttt gtatcacaag agcgaagacc aacttcagcg cactctcgag gacgccgagg 23940
ctctcttcaa caagtactgc gcgctgactc ttaaagagta gactgcgcgc gcttggcgag 24000
aaaaggcggg aattacgtca cctcttggcc acacctgtgc ttcattatga gtaaagaaat 24060
tcccacgcct tacatgtgga gctatcagcc ccagatggga ttggccgctg gcgccgccca 24120
ggactactcc acccgcatga attggctcag cgccggtccc gcgatgatct cacgggttaa 24180
tggtgtgaga gagcaccgaa accagatact cctagaacag tccgccctca ccgccactcc 24240
ccgcaatcac ctcaaccccc gtaattggcc cgccgccctg gtgtaccagg aaactcctgc 24300
tcccactaca gtactacttc ctcgtgacgc ccaggccgaa gttcagatga ctaactcagg 24360
tgtacagctg gcgggtggtg ccaccctgtg tcgtcaccgg ccaagaccgg gtataaaggg 24420
cctggtgatc agaggccgag gtattcagct caacgacgag tcggtgaact cttcgcttgg 24480
tctgcgacca gacggcatct tccaaatagc tggttgtggg agatcttcct tcactcctcg 24540
tcaggctgtc ctgactttgg agagttcgtc ctcgcagccc cgctcgggcg gcatcgggac 24600
tctccagttc gtggaggagt ttactccctc ggtctacttc aaccccttct ccggttctcc 24660
tgggctttac ccggacgagt tcatcccgaa ctacgacgcc atcagtgaag cggtcgacgg 24720
ctacgattaa tgtctaatgg tggcgcggct gagctagctc gactgcgaca cctagaccac 24780
tgccggcgct ttcgctgctt tgctcgggat ctctgcgagt tcatctactt cgagtaccct 24840
gacgaacatc ctcagggacc tgcccacgga gttcggatta ccattgaagg ggctatcgat 24900
tctcacctgc ttcggatctt caccgctcgg ccagtgctag ttgagcgcaa ccagggcgac 24960
accaccatct ccctctgctg catttgtgac aaccccggat tgcatgaaag cttttgttgt 25020
cttctttgta ctgagtataa taaaagctga aattagagac tactccggac tctcttgtcg 25080
tctgaacaac accaaccaga cccttcactt cagcgggaac cagactactc ttcactgtaa 25140
ggcttataac tataagtatc ttacttggat atacaaagga acaccgtttg ctgtggtaaa 25200
caggtgctcc aacgacggtg ttctcctcac cttcctaggc aacttctcca actttacctt 25260
ttctgttcgc agaaacaagc ttaccctcct tcagccctac tttcctggga tctatacctg 25320
cctcagtgga ccttgcaacc acacttttca cctgattgaa aactctaccc ttaccttccc 25380
agcgccaatc cctactaaca gctcggagtc caactcttcc attaccgctg atactaacac 25440
tcctaaaacc ggaggtgagc tccgcagcct tcccccggct gcagataacc cttgggtggt 25500
agcgggattt gtagcgctag gaatagttgc gggtgggctc gcgttcgtcc tctgctacct 25560
ataccttacc tgctgctcat atttagtagt actgtgctgt tggtttagaa aatgggggcg 25620
ctactaatca cacttgcttt actttcgctt ttgggtctga gctcggctaa tagcgagaaa 25680
ccaagctgtc tagaaacaaa ctctccagac tgtgtggttc ctcatgggct ctcagaccca 25740
gctgatgatc catgcttaac ttttgaccca gaaaaaaact gctcggtgac tatgcagccc 25800
tatgcttaca tgtgcacatc tgttataaag tgcggatggg gctgtaaacc gattgaaatt 25860
acccacaaag gcaaaacctg gaataatagt ttgtttaaca catggcagcc tggagacgag 25920
cagtggtata cggccggcca ctggtggaga tgactgaccc catggaaaac tcctctgcca 25980
acgacctgga catggacggc cgttcatctg agcagcgact ggtccagatg cgcattcgcc 26040
agaagcagga acgcgccgcc agagagctca aggatgccat tgaaattcac ctgtgcaaga 26100
agggcatctt ttgcttggtt aagcaagcaa agatttctta tgaaatcact gacaacgacc 26160
accgcctgta ttatgagctc ggtccacagc ggcagaaatt cacctgcatg gttggagtca 26220
accccatagt catcactcag caggctgcag aaattaaagg gtgcatccac tgttcctgtg 26280
attcccaaga atgcgtccac accatagtca agaccctctg cggccttcga gatcttcttc 26340
caatgaacta accccttccc ccaacccaat aaaacattgg ttttaatcat aataaaaaat 26400
cacttacttt aaatctgaaa cagtgtctcc gtccaagttt tcttgtagca ccacttcact 26460
cccctcttcc cagctctggt actgcaagcc ccggtgggct gcaaactttc tccacacctt 26520
aaaagggatg tcaaattcct cttgtccaac aatcttcatt gtctcttcct agatgtccac 26580
aaagcgcgcg cgggtggaag atgactttga ccctgtctac ccatacgatg ctgagctggc 26640
accgtctgta cccttcatcg cccctccctt cgtttcgtca gacggatttc aagaaaaacc 26700
cctgggagtt ctgtccctaa gactagccaa cccagtcact actaaaaatg gggaactcac 26760
acttaaactg ggagatgggg tgggcataga ctcagatgga aacctcacag cacagacagt 26820
tactaaagca acatcccccc ttactgtttc caataacgca attgcactta acatggacaa 26880
acctttttac agtagcaatg gaaaactatc cttacaagtt acatcaccat taaagatagt 26940
cgactcttta aatacattgg ctattggcta tgggcaaggc ttaggactaa acaatagtgc 27000
tcttgctgtg caattagcat ctccccttac atttgacagc aacagcaaaa ttaaaataaa 27060
tttgggaagc gggccattaa aaattaatgc gaataaactg tcaattaact gcctaagggg 27120
tgtatatgta acaactgacg gaacttccat tgaaacaaat ataagctggg caaaaggaat 27180
gaggtttgaa ggtaatgcca tggctgtaaa cgttgacagc accaaaggtc tacaatttgg 27240
cactaccagc acagaatcag gagtcactaa cgctttccct atccagttaa agattggatc 27300
tggtcttagt tttgacagca caggagcact tgtagcttgg gataaggata atgacaagct 27360
tacactgtgg acaaccgctg acccatcacc taattgtacc atatatacag acaaggatgc 27420
taaacttaca ctttgtctta caaaatgtgg cagtcaaata ctaggcagtg tttcagtact 27480
ggctgttaaa gctggaaccc tacagccaat cagtgaaaaa ataggtactg ctttggtttc 27540
actaaaattt aataacaacg gtgtattgtt aagcaactcc acattaagta atgaatactg 27600
gaactacagg aagggagatg tcacaccagc cgaagcctat actaatgctg tgggttttat 27660
gccaaacatc aaggcatatc ctaaaaacac aaactctgcc tcaaaaagcc acattgtagg 27720
acaagtgtac cttaatggag atgaaactaa accaatgcat ttaatcatta catttaatga 27780
aaccagtgat gaaacatgca catattccat aacgttccaa tggaaatgga acattggaac 27840
atacaccagc gacacccttg caacaagctc ctttaccttt tcttacattg cccaagaata 27900
aaaactgcag acaacaataa agtttaaatg ttttatttaa acagtttcac agaaccctag 27960
tattcaacct gccacctccc tcccaacaca cagagtacac agtcctttct ccccggctgg 28020
ccttaaaaag catcatatca tgggtaacag acatattctt aggtgttata ttccacacgg 28080
tttcctgtcg agccaaacgc tcatcagtga tattaataaa ctccccgggc agctcactta 28140
agttcatgtc gctgtccagc tgctgagcca caggctgctg tccaacttgc ggttgcttaa 28200
cgggcggcga aggagaagtc cacgcctaca tgggggtaga gtcataatcg tgcatcagga 28260
tagggcggtg gtgctgcagc agcgcgcgaa taaactgctg ccgccgccgc tccgtcctgc 28320
aggaatacaa catggcagtg gtctcctcag cgatgattcg caccgcccgc agcataaggc 28380
gccttgtcct ccgggcacag cagcgcaccc tgatctcact taaatcagca cagtaactgc 28440
agcacagcac cacaatattg ttcaaaatcc cacagtgcaa ggcgctgtat ccaaagctca 28500
tggcggggac cacagaaccc acgtggccat cataccacaa gcgcaggtag attaagtggc 28560
gacccctcat aaacacgctg gacataaaca ttacctcttt tggcatgttg taattcacca 28620
cctcccggta ccatataaac ctctgattaa acatggcgcc atccaccacc atcctaaacc 28680
agctggccaa aacctgcccg ccggctatac actgcaggga accgggactg gaacaatgac 28740
agtggagagc ccaggactcg taaccatgga tcatcatgct cgtcatgata tcaatgttgg 28800
cacaacacag gcacacgtgc atacacttcc tcaggattac aagctcctcc cgcgttagaa 28860
ccatatccca gggaacaacc cattcctgaa tcagcgtaaa tcccacactg cagggaagac 28920
ctcgcacgta actcacgttg tgcattgtca aagtgttaca ttcgggcagc agcggatgat 28980
cctccagtat ggtagcgcgg gtttctgtct caaaaggagg tagacgatcc ctactgtacg 29040
gagtgcgccg agacaaccga gatcgtgttg gtcgtagtgt catgccaaat ggaacgccgg 29100
acgtagtcat tctcgtactt tgagtggcaa aaccttgctc tcgaacagca cacgtctcgt 29160
cgcctcctgt cccttctctt cgccttttca gtgtgatagt tgtaatacag ccattcacga 29220
agctcagtca gaagatcttc agcgtctgtt gtcaaaaaca atccatccaa tctgattgct 29280
ttcaaaacat cacaaacagt cgaataagcc aaacccatcc aggcaatgca attattttgg 29340
ttatccacaa tgggaggggg cggaagacat ggaagaggca taattaattt tttaatccaa 29400
tcgatcacgc agcacttcaa aatgaagatc gcgaaggtga cacctttcac ccccactgtg 29460
ttgatgaaaa ataacagcca agtcaaaatt gatgcggttt tcaaggtgct cgactgtagc 29520
atcaagcaga gcttccacac gcacgtccac aaataacaga atagcaaaag cgggaggagg 29580
aagtaaatcc tcaatcatca tagtacagtc catcaccatc cctaaataat tttcatcctt 29640
ccagccttgg actatatttt taaactgctc ttgtaaatcc aaaccacaca tgtggaaaag 29700
ttcccaaaga gctccctcaa ctaccattct taagcacacc ttcatagtga caaaatatct 29760
tgttcctctg tcacctgcag caaattacaa agtccaatat taggatctat gcccagagat 29820
ctaagctcat ccctcaattc caactgtaaa aaggcttcca gatctgccct aacttgttca 29880
gccagtgggc tccctggaat aagcgtggga gaagccaaac tgcaaaacag acgcatgccg 29940
ccataattac caccagaaaa cactacgtta cagtatgcat gctgattcat tccagtaatt 30000
tcatccagtg tattggatac aaaaaaaggc aagcactctc tcactaattg tattatggag 30060
acattatcac acaggtaaca atttaaaggt tgtggaacaa taatgcagta agtaaccacg 30120
gtgcgctcca acatggttag taatttttag ttctgaaaaa caaaacatac aaaaaattat 30180
atcatactca tttggcgaac tggtggaaaa atgaccctat ctagcacaag gcaagccact 30240
ggatcaccaa tgcgcccctc ataaaacctg tcatcatgat taaaaagcaa caccgaaagc 30300
tcttccctat gtcctgcatg aatgattcta gctgaggaat ataagccagc gcaattagta 30360
tctgttaaag aaaaaaaacg gccaacatag cctctaggaa ttagcacact taatcttaaa 30420
gacattactg ccatccccct tggatttaag gtaaaattta caggagcata gaaaatatac 30480
tgatttccct cctgcacagg cagcatagca ccaggtccct ctaaaaacac acacaaagct 30540
tctgcagcca tagcttaccg cgcaaaccag gcacagcagt gagctaaaag gacaaagctc 30600
taactcacta gccaacctgg cgcacaatat atagttagtc cttacactga cgtaaccgac 30660
caaagtctaa aaaccccgcc aaaaatacac acacgcccaa aaaacgcccc gtgagtcaaa 30720
aaacagtttc acttcctcgt tacacccaaa acgtcgtcac ttccggattc ccacggttcg 30780
tcacttccgg agctccttgc ttaattaacc ccgcccaaaa cgtcatcgtc cgcgtcacgc 30840
cgccccgccc cgcgaccgtt gaccccgggc caatcaccgc acatcccgca aaattcaaac 30900
tcgtctaatt tgcatattgg cacactgccc atataaggta tattattgat gatgatttaa 30960
atcatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct 31020
cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 31080
cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 31140
acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 31200
ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 31260
ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 31320
gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 31380
gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 31440
ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 31500
actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 31560
gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 31620
ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 31680
ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 31740
gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 31800
tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 31860
tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 31920
aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 31980
aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 32040
tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 32100
gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 32160
agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 32220
aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag 32280
gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 32340
caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 32400
cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 32460
ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 32520
ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac 32580
gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 32640
cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 32700
gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 32760
caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 32820
tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 32880
acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 32940
aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 33000
gtatcacgag gccctttcgt cttcaagaat tgatttaaat 33040
<210> 9
<211> 32701
<212> DNA
<213> 人工序列
<220>
<223> pBLY6.dE1.dE3.5IXP
<400> 9
catcatcaat aatatacctt atatgggcag tgtgccaata tgcaaattag acgagtttga 60
attttgcggg atgtgcggtg attggcccgg ggtcaacggt cgcggggcgg ggcggcgtga 120
cgcggacgat gacgttttgg ggaggaggag ctatgttgca agtaatcgtg ggaaatgcga 180
cgtaaaacga ggtggagttt aaacacggaa gtagacaatt ttcccgcgct gtttgacagg 240
aaatgatgtg tttttgggcg gatgcaagtg aaaattctcc attttcgcgc gaaaactaaa 300
tgaggaagtg aaattctgag taattctgag gttatcacag ggcggagtat ttaccgaggg 360
ccgagtagac tttgacccat tacgtggagg tttcgattac tctatttttc acctaaattt 420
ccgcgtactg tgtcaaagtc cgtgttttta cgcgatcgcg gtactgaaat gtgtgggcgt 480
ggcttaaggg tgggaaagaa tatataaggt gggggtctta tgtagttttg tatctgtttt 540
gcagcagccg ccgccgccat gagtgggagc gcttcctttg aagggggcgt ctttagccct 600
tatctgacgg ggcgtctgcc tcattgggct ggagtgcgtc agaatgtgat ggggtctaca 660
gtggatggaa gacctgttca gcctgctaat tcttctactc tgacttatgc tactatgact 720
tcctcgcctt tggatgcagc tgcagctgct gccgcttctg ctgccgccaa cactgttcgg 780
gggatggcct tggagatggg gtattatgga actgtagtgg ccaacaccac taccccaaat 840
aaccccacag ccttgaatga ggacaagctg ctagttctca tgtcccagct ggagtctttg 900
acccaacgcc tgggcgatct agctcagcag gtgtcccagc tgaaggagca gactcaagct 960
gcaattacca ctgcgagggg aaattaaaaa aattcaaaga atcaataaat aaaccgagac 1020
tttgttgatt ttaaagtgtg tcattcttta tttaattttt cgcgcgcgat atgccctgga 1080
ccaccggtct ctatcattga ggacacggtg gatcttttct agaacccgat agaggtggga 1140
ttggatgttg aggtacatgg gcataagacc atctttgggg tgtagatagc tccactgcag 1200
agcctcatgc tccggggtgg tgttgtatat aacccagtca tagcatgggc gttgggcatg 1260
atgttgcaca atatctttaa ggaggagact aatggccact gggagaccct tggtgtaagt 1320
gtttacaaat ctattaagct gggacgggtg catccgaggt gagataatgt gcattttgga 1380
ttggattttt agattggcaa tgtttccccc tagatctctc ctgggattca tgttatgcaa 1440
gaccactaga acagtgtatc cggtgcactt agggaatttg tcatgaagtt tggaggggaa 1500
agcatgaaaa aatttagaca cacccttgtg tcctcccaag ttctccatgc actcatccat 1560
aataatggca atgggcccat gggcggcggc acgggcgaac acgttcctgg gatctgacac 1620
atcatagttg tggtcttggg tcaggtcatc ataagccatt ttaataaact tggggcggag 1680
ggtgccagat tgggggatga atgttccctc gggccccgga acatagtttc cttcacatat 1740
ttgcatttcc caggctttta gttcagaggg ggggatcatg tccacctgtg gagcgatgaa 1800
gaagacggtc tcgggggcgg gggtgattaa gtgggaggac agcaagttcc taagcagctg 1860
tgacttgcca cacccagtgg gaccgtagat gacccctata acaggttgca gatggtagtt 1920
tagggaaaga cagctgccgt cctctcgcag gaggggggcg acctcgttca tcatttccct 1980
cacatgcatg ttttcccgca caagttccga taggaggcgc tctccaccca gggaaaggag 2040
ttcttgaaga gatgagaaat ttttcaaggg ttttaagcca tcagccatgg gcattttgga 2100
gagggtttgt tgcaagagtt caaggcggtc ccagagttcg gtgatgtgtt ctatggcatc 2160
tcgatccagc atacttcctc gtttctgggg ttgggacggc tgcgggagta tggaaccagg 2220
cgatgggcgt ccagcgctgc cagtgtccgg tccttccacg gtcgcagcgt ccgagtcagg 2280
gtcgtttccg tcacggtgaa ggggtgcgcg cctggctggg cgcttgcgag ggtgcgcttc 2340
aggctcatcc tgctcgtgga gaaccgctgc cgttctgcgc cctgtgcatc ggccaggtag 2400
caattaacca tgagttcgta gttgagcgcc tctgccgcgt ggcctttggc gcgcagctta 2460
cctttggaag tcttctgaca ggtgggacag tagagacact tgagagcata gagttttggg 2520
gctagaaaga ccgattctgg ggagtatgca tcggccccac aggaggcgca gacggtttcg 2580
cattccacca gccatgtaag atcgggctcg ttggggtcaa aaacaagttt tccgccatgt 2640
tttttgatgc gtttcttacc tttgctttcc atgagttcgt gcccccgttg ggtgacaaag 2700
aggctgtccg tgtccccgta gactgacttt atgggcctgt cctcgagcgg cgtgccgcgg 2760
tcctcttcgt agaggaactc ggaccactct gagacgaaag cacgtgtcca ggccagcaca 2820
aaggaggcta tatgggaggg gtagcgatcg ttgtcaacca aggggtctac tttttccaag 2880
gtgtgtaaac acatgtcccc ttcttccaca tccaggaagg tgattggctt gtaagtgtat 2940
gccacgtgac ctggggtccc agacgggggg gtataaaagg gggcgggtct ctgctcgtcc 3000
tcactgtctt ccggatcgct gtccaggagc gccagctgtt gaggtaggta ttccctctcg 3060
aaggcgggca taacctccgc actcaggttg tcagtttcta ggaacgagga ggatttgata 3120
ttgacagtgc ctgccgagat gcctttcatg agactgtcgt ccatttggtc agaaaagaca 3180
atctttttgt tatcaagttt ggtggcgaag gatccataca gggcattgga aagcagtttg 3240
gcaatggagc gcatggtttg gtttttttct ttgtctgcgc gctctttggc ggctatgttg 3300
agttggacat attcgcgggc cagacatttc cattgtggaa atatggtagt taattcatct 3360
gggacgattc tgactttcca gcctctgtta tgcagggtaa tcagatccac actggttgcc 3420
acttctcctc taagtggttc attagtccag catagtcgcc ccccttttcg agaacagaaa 3480
gggggtaggg gatctagcat gagttcgtct ggggggtctg catctatggt gaaaatccca 3540
ggaaggagat cttcgtcaaa atagctgatg gtggcggggt catccagaga catttgccat 3600
tctcgagcag ccagagcgcg ctcgtagggg ttaaggggag tcccccatgg catgggatgg 3660
gtgagtgcag aagcatacat gccacagatg tcatagacat agagcggctc ttccagaatc 3720
cctatgtaag tgggataaca tcgcccccct ctgatgctgg ctcgcacata atcatagagt 3780
tcatgtgagg gcgctagaag acccgagccc aggttggtgc ggttgggttt ttctgctctg 3840
tagaggatct ggcgaaagat ggcatgggag tttgatgaga tggtgggtct ttggaagatg 3900
ttgaaatggg catgaggcag tcccacagag tcccttatga agtgagcata ggagtcttgc 3960
agtttggcca ccagctcggc ggtgaccagc acatccaaag cacagtagtc gagggtctct 4020
ttgatgatgt catagttagg ttcccctttc ttttcccaca gctcgcggtt gagaaggtat 4080
tcttcgcgat ccttccagta ctcttcgagg gggaacccgt ccttgtctga acggtaagaa 4140
cccagcatgt aaaattgatt gacagctttg taggcacaac accccttctc cacggggagt 4200
gagtatgctt gcgcggcttt gcgcagagag gtgtgagtaa gggcgaaagt gtccctgacc 4260
atgactttga ggaactgatg cttaaagtct atgtcatcgc aggccccctg ctcccacagt 4320
tggaagtcca ctcgcttttt gtaggcggga ttgggcaaag cgaaagtaac atcgttgaat 4380
aggatctttc cagccctggg catgaagttg cgagtaatgc gaaaaggctg aggcacttct 4440
gccctgttgt tgataacttg ggcagccaag acgatctcgt caaagccgtt gatgttgtga 4500
cccacaatgt aaagttctac gaagcgtggg cgtcccttga tgtggggcag ttttttaagc 4560
tcttcgtagg tcaagtcgtc agggtcagcg attccatatt gctccaaagc ccagtcaggc 4620
aggtgaggat tagcatgaag gaaagaggtc caaagatcca cggccagagc tgtttgtaag 4680
cggtctctgt actgacggaa atgtcggcct accgccattt tttcaggagt aacacagtaa 4740
aaggtgcgcg ggtccttttc ccagcgatcc cattgaagtt gcaaggctag gtcgtgggcg 4800
aggttgacga gctgttcgtc ccccgaaagt ttcatgacca gcatgaaagg gacaagctgc 4860
ttgccaaagg accccatcca ggtgtaggtt tccacatcgt aggtgaggaa gagcctttct 4920
gtgcgaggat gagaaccgat cgggaagaac tggatttcct gccaccagtt ggaggaatgg 4980
ctgttgatat gatggaagta gaactcccta cggcgcgccg agcattcgtg cttgtgcttg 5040
tacagacggc cacagtactc gcagcgctgc acgggatgca cctgatgaat gagctgtacc 5100
tggcttcctt tgacaagaaa tttcagtggg aagttgaggc gtggcgtctg catctcgtgt 5160
tgtattacgt cctggctatt ggtctggcca tcttctgtct cgatggtggt catgctgacg 5220
agcccgcgcg ggaggcaggt ccagacctcc gcgcggacgg gtctgagagc gaggacgaga 5280
gcgcgcaggc cggaactgtc cagggtcctg agacgctgcg gagtcaggtc agtagggaga 5340
gtacataggt ttacttgcat aagtttttcc agggcatgtg ggaggtcaag atgatatttg 5400
atttctactg gcgagttggt ggagacatcg atggcttgca gggtcccgtg cccctggggt 5460
gctaccaccg tccctttttt tttcttgatc gggggcggtg ttgcttcttg catggtaagg 5520
tcgtcttcta gaagcggcgg cgaggtcgcg cgccgggtgg cagtggcggt tctggacctg 5580
gaggtagagg cggtagaggt acgtcggcgc cgcgcgcggg taggttctgg tactgcgccc 5640
tgagaagact tgcgtgagcg acgacgcggc ggttgacgtc ctggatctga cgcctctggg 5700
tgaatgctac cggacccgtg agcttgaacc tgaaagagag ttcaacagaa tcaatttcgg 5760
tatcgttgac ggctgcctgc cgcaggattt cttgtacgtc gcccgagttg tcttggtagg 5820
cgatctcggc catgaactgc tcgatctctt cttcttggag atctccgcgg cccgctcgtt 5880
ctacggtggc agcaaggtcg ttggagatgc gccccatgag ctgtgagaat gcattcatgc 5940
ccgcctcgtt ccagacgcga ctgtagacca cggctccctc gggatctctg gcgcgcatga 6000
ccacttgggc gaggtttagt tccacgtgtc tggtgaagac cgcatagttg cagagacgct 6060
ggaagaggta gttgagcgtg gtggcgatgt gctcggtgac aaagaaatac atgatccagc 6120
gacgaagcgg catctcgctg atatcgccca gggcttccaa ccgttccatg gcttcgtaaa 6180
agtccacggc gaagttgaaa aactgggagt tgcgagcgga cacggtcaac tcctcctcca 6240
gaagacggat gagctcggcg atggtggcgc gcacttcgcg ctcaaaggct cccgggatct 6300
cttcctcctc ttcttcttcc aactcttcct ccactaacat ctcttctact tcctcctcag 6360
gcggcggggg tggaggaggg ggcgcgcggc gacgccggcg acgcacgggc agacgatcga 6420
tgaagcgttc gatcacttct ccgcggcggc gacgcatggt ctcggtgacg gcgcgcccgt 6480
cctccctggg tcgcagagtg aagacgccgc cgcgcagctc cctgaaatgg tgactgggag 6540
ggtccccgtt tggtagggac agggcactga tgatgcatct tattaattgc cctgtaggga 6600
ctccgcgcaa ggacctgagc gtctcgagat ccacgggatc tgaaaatctc tgaacgaagg 6660
cttcgagcca gtcgcagtcg caaggtaggc tgagcactgt ttcttcgggg cgggctgctg 6720
agctagaggg ttgtacgatg ctgctggtga tgaagttaaa ataggcagtt ctgagacggc 6780
ggatggtggc gaggagcacc aggtctttgg gtccggcttg ctggatgcgc aggcggtcgg 6840
ccattcccca tgcattatct tggcacctgg ccagatcttt atagtagtct tgcatgagtc 6900
gctccacggg cacttcttct tcgcccgctc tgccgtgcat gcgtgtgagt ccgtaccctc 6960
tctgtggttg gacgagcgcc aggtcggcaa cgaccctttc ggctagaatg gcttgctgca 7020
cctgggtgag ggtgttctgg aaatcatcaa agtccacaaa gcggtggtag gcccccgtgt 7080
tgatggtgta ggagcagttg gacatgaccg accagttgac tgtctggtgt cctggtcgta 7140
cgagttccgt gtacctgagc cgcgagtatg cgcgggagtc gaagatgtaa tcgttgcagg 7200
ttcgcaccag gtactggtag ccgatgagga agtgaggcgg cggctggcgg tagagaggcc 7260
atcgttcggt ggcgggcgcg ccgggcgcta ggtcttctag catgagacgg tggtatccgt 7320
agacgtacct ggacatccag gtaataccgg cggcggtggt ggaggcgcgc ggaaactctc 7380
gcacgcggtt ccagatgttg cgcagcggca tgaagtagtt catggtgggc acggtctggc 7440
ccgtgaggcg cgcgcagtca ttgatgctct agatacgggc aaaaacgaaa gcgttgagcg 7500
gttcccttcc gtggcctgga ggaacgcgaa cgggttaggt cgcagcgtac cctggttcga 7560
gactaaagaa agcgagcaac tcgaaccggc agagtcgcgg ctaacgggta ttggcaatcc 7620
cgtctcgacc caagccagca aatccaggat acggatgggg gccccttttg tttttcaggg 7680
catgagtcac cggttaaggt ttacaacggc tgtttcatgc ctttagaagt ggctcgcgcc 7740
cgtagtctgg agaatcaatc gccagggttg cgttgcggcg tgccccggtt cgagcctgca 7800
gcttgagtcg gccggtgacc gcggcaaacg agggcgtggc ggccccgtcg tttctaagac 7860
cttgctagcc gacctctcca gtttacggga acgagccccc ttttattttt tttgtttttg 7920
ccagatgcat cccgtactgc ggcagatgcg cccacagccc ccacagcagc agcagcaggc 7980
tggcctacct tctctacctc agccgctacc tgcaactacc gcggtggccg ctgtaagcgg 8040
ggccggacag caggcggctc ctcaatatga attggacttg gaagagggcg agggattggc 8100
aagattgggg gcgccctcgc ccgagcgcca cccgcgggtg cagatgaaaa aggacgttcg 8160
cgaatcttac gtgcccaagc agaatctgtt cagagacaga agcggcgagg agcccgagga 8220
gatgcgcgcg tcccgtttta acgcgggtcg cgagctgcga caaggactgg atcgaaaacg 8280
ggtgttgagg gatgatgatt ttgaggtgga tgaaatgaca gggatcagcc ccgctcgcgc 8340
tcacgtggct gcagctaatc tggtgacagc ttatgagcag accgtgaagg aggaaagcaa 8400
cttccagaaa tcattcaata accacgtgcg caccctgatc gcacgcgagg aggtgaccct 8460
gggcctgatg cacctgtggg atctgctgga agccatagtg cagaacccca ctagcaaacc 8520
cctgactgct caactgtttc tggtggtgca gcacagcagg gataatgagg cattcagaga 8580
ggcgctgctg aatatcactg aacctgaggg gagatggctg ctggatctgg tgaatatcct 8640
gcagagcatt gtagtgcagg aacgcagctt gcctttgtcc gagaaggtgg cggcgatcaa 8700
ttactctgtg ctgagtctgg gcaaatacta tgccaggaag atctacaaaa ccccttacgt 8760
gcccatagac aaggaagtga aaatagatgg gttttacatg cgcatgaccc tgaaagtgct 8820
aaccctgagc gatgacttgg gagtgtaccg caacgacagg atgcaccgcg cggtgagcgc 8880
cagcaggagg cgcgagctga gcgacaaaga attaatgcac agcttgcaac gagccctgac 8940
gggagccggg acggaggggg agaactactt tgacatgggt gcagacttgc attggctgcc 9000
tagtcgcagg gcattggaag cggcaggcga tgggccctat gtagaggaag tagtagacga 9060
ggacgatgag gagggcgagt acctggaaga ctgatggcgc gacccgtatt tttgctagat 9120
ggaacaggcg ccggaccctg cgatgcgggc ggcgctgcag agccagccgt ccggcattaa 9180
ttcctcggac gattggaccc aggccatgca acgcatcatg gcgctgacga cccgcaaccc 9240
cgaagccttt agacagcagc ctcaggccaa ccgcctttcg gccatcctgg aggccgtggt 9300
gccctctcgc tccaacccca cccacgagaa ggttctggcc atcgtgaatg ccctggtgga 9360
gaacaaggcc atccgctccg atgaagccgg gctggtatac aacgccttgc tcgagcgcgt 9420
ggctcgctac aacagcagca atgtccagac taacctggac aggatggtga ccgacgtgcg 9480
cgaggccgtg tcccagcgcg aacggttcca tcgcgagtct aacctgggtt ccatggtagc 9540
gctgaacgct ttcctcagtt cccagcctgc caatgtgccc cggggacagg aagactatac 9600
caactttatt agcgccctga gactcatggt agccgaggtt cctcagagcg aggtgtacca 9660
gtccggtcca gactactttt tccagacaag caggaacggt atgcagacag tgaacttaag 9720
ccaggctttc aagaacctgc aagggctgtg gggagtccaa gctccagtgg gcgacagggc 9780
gaccgtgtcg agcctgttga ctccaaattc ccgtttgctg ctgctgctgg tgtccccctt 9840
cactgacagc ggcagcataa acagaaactc ctacttgggc tacctgataa acttgtatcg 9900
cgaagctata ggtcaggccc acgtggacga acagacctat caggagatca ctaatgtgag 9960
tcgcgctctg ggccaggacg accctggaaa cctggaagct actctaaact ttctgctgac 10020
caaccgctcg caaaaaatcc ctcctcagta tacattaact gcggaggagg aacggatctt 10080
gagatacgtg cagcagagcg tgggtctgtt cctgatgcaa gagggtgcga cccctagcgc 10140
cgcgcttgat atgacagcgc gcaacatgga gcccagcatg tatgccagca acagaccatt 10200
cattaataaa ttgatggatt acttccatcg cgcggccgct atgaactctg attacttcac 10260
caatgctatt ctgaaccccc attggctgcc tccgcctggt ttttatactg gcgagtatga 10320
catgcctgac cccaacgatg ggttcttgtg ggacgatgtg gacagcgtgg cgttctcgcc 10380
taccgctcct cgtacttttt ggaagaagga aggtagtgac agaagaccct cctccgtgct 10440
gtcaggacgt gagggtgctg ccgcggcggt ccccgatgct gcaagcccct ttcccagtct 10500
gccattttca ctaaacagcg tgcgcagtag cgagctgggg agaataaccc gccctcgctt 10560
gctgggcgag gacgagtatt tgaatgactc cctactgaga cccgagcggg aaaagaactt 10620
ccctaataat gggattgaaa gcctggtgga taagatgagc agatggaaga cctatgccca 10680
ggagcacaga gatgagccta gaatcttggg tcctacagta ggcacccgca gacgccagcg 10740
ccatgataga cagcggggtc tggtgtggga cgatgaggat tctgcagatg acagcagcgt 10800
gttggacttg ggcgggaggg gaggtgtggg caacccgttc gcacacttgc gtccccgtat 10860
tggacgcatg atgtaaaagt gaaaataaaa aaggaactca ccaaggccat ggcgaccagc 10920
gtgcgttcgt tctttctgtt gttgtatcta gtatgatgag gcgcaccgtg ctaggcggat 10980
cggtggcgta tccggagggt cctcctcctt cgtacgaaag cgtgatgcag caggtggcgg 11040
cggcggcgat gcaacccccc ttggaggctc cttacgtgcc cccgcggtac ctggcaccta 11100
ccgaggggag aaacagcatt cgttattcgg aactcacacc cttgtatgac accacccggt 11160
tgtacctggt ggacaacaaa tcggcggaca ttgcctcgtt gaactatcag aacgaccaca 11220
gcaacttctt gacaacggtg gtgcagaaca atgactttac ccccacggag gccagcaccc 11280
agaccatcaa ctttgacgag cgctcccggt ggggcggtca gctgaagacc atcatgcaca 11340
ccaacatgcc caacgtgaac gagttcatgt ttagcaacaa gttcagggct agggtgatgg 11400
tgtccagaac cacacctaaa gaggtgacag tcacaacaga ctatgatggt agtcaggaca 11460
tcttggaata cgagtgggtt gactttgagt taccagaagg caacttctct gccaccatga 11520
ccatagacct gatgaataat gcaattgttg ataattacct aaaagtgggt agacagaatg 11580
gggtactgga gagtgacata ggtgttaagt ttgacactag gaactttagg cttggttggg 11640
acccagtgac agagttggtc atgcctgggg tctacaccaa tgaagctttc catcctgaca 11700
tagtcctact acctggctgc ggagtggact tcactgagag ccgcctcagt aatctgctag 11760
gcattagaaa gaaacagcca ttccaggaag ggttccagat catgtatgag gatctggagg 11820
gtggtaacat ccccgccctg cttgatgtaa atgcatatga gaagagcaag gaagataata 11880
caaccaccac aaatgaagct gtggccgcgg cttcatctac tgaagccaaa gctgtggtag 11940
atgcttccac ttcaacagaa aacaccactg atgaaaaagt caccagggga gatacatttg 12000
ccacccctga acaagagaag gcagctgagg cagagtctga tattatgctt ctgtccaccg 12060
atgaaaacga aactaaaaaa caactggtta ttcgagcggt gaccaaggat agtaaggaca 12120
ggagttataa tgtattgtca gatggaaaga acacagctta ccgtagttgg tacctggcat 12180
acaattatgg cgaccgtgag aaaggggtgc gttcttggac actgcttacc acctcggatg 12240
tcacctgcgg cgtggagcaa gtctattggt cgctaccaga tatgatgcaa gatccagtca 12300
cctttcgctc cacacgccaa gttagcaact acccagtggt gggcgcagag ctgctcccag 12360
tgcattccag aagcttctac aacgagcaag ccgtctactc gcaacagctc cgccagtaca 12420
cctcgctcac gcacgtcttc aaccgcttcc ccgagaatca gatcctcgtc cgcccgcccg 12480
cgccaaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 12540
cgctgcgcag cagtatccgg ggagtccagc gcgtgaccgt tactgacgcc agacgccgca 12600
cctgtccata cgtatacaag gccctgggca tagtcgcgcc gcgcgtcctt tcaagccgca 12660
ctttctaaaa aaatgtccat tctcatctcg cccagtaata acaccggttg gggcctgcgc 12720
acacctagca agatgtatgg aggcgctcgc agacgctcca ctcagcaccc tgtgcgcgtg 12780
cgcgggcatt tccgcgctcc ctggggcgcc ctcaagggac gctctcgtac taggaccacc 12840
gttgacgatg tgatcgacca ggtggtcgcc gatgcacgta actatacccc cgcagccgca 12900
cctgcatcca ccgtggatgc ggtcattgac agcgtggtag ccgatgcgcg cgcctatgct 12960
cgcgccaaga gcaggaggcg gcgtattgcc aggcgtcacc gagctactcc agccatgcga 13020
gctgcaagag ctttattgcg gagagccaga cgtgtggggc gaagagccat gcgtagagcg 13080
gccagacgcg cggcttcagg tgccagcgca ggcagggtcc gcaggcgcgc ggctacggcg 13140
gcagcggcgg ccatcgctag catgaccaaa ccacgaagag gcaatgtgta ttgggtgcgc 13200
gacgccgcca ccggccagcg cgtgcccgtg cgcacacgcc cccctcgcac ttagaagata 13260
ctgagcagtc tccgatgttg tgtcccagcg gcgagatgtc caagcgcaaa ttcaaggaag 13320
agatgctcca ggtcatcgcg cctgagatct acggtcctgc ggtgaaggat gaaaaaaagc 13380
cccgcaagat caagcgggtc aaaaaggaca aaaaggaaga agatggtgat gatgggctgg 13440
tggagtttgt gcgcgagttt gccccaagga ggcgcgtgca gtggcgcggg cgcaaagtgt 13500
ggccggtgtt gagaccgggg accacagtgg tctttacgcc aggcgagcgc tccagcaccg 13560
tttccaaacg ctcttatgat gaggtgtacg gggacgatga tattctcgag caggcggctg 13620
atcgccttgg cgagtttgca tatggcaaac gcagccgctc gggagccaag gaagaggcat 13680
tgaccatccc cttggatcat ggaaatccca ccccaagcct caaacccgtg accctgcaac 13740
aagtgctgcc cacgccgcca cgcaagggca tcaagcgcga gggcgaggat ctgtatccca 13800
ccatgcagct gatggtgccc aagcgccaga agctggaaga cgtgctggag aaaatgaaag 13860
tggatcctga aatccagcct gaagtcaaag tgaggccaat caagcaggtg gcgcccggtt 13920
tgggggtaca aaccgtggat atcaagatcc ccaccgagtc catggaaatt caaaccgaac 13980
ccatgaagcc cacctccagc accattgagg tgcagacgga tccttggatg cccgcgcctg 14040
ctcctgttac cactactact cgaagaccta gaagaaagta tggttcagcc aacctgataa 14100
tgccaaacta tgctctgcat ccatcaatca tacccactcc tggctaccgc ggcactcgct 14160
actaccgcag tcacagcacc cgccgacgta aagcacctgc cacccgccgc cgtcgccgcc 14220
gccgtgccac tagcaaactt accccctcgg ctatggtgcg gagagtgtac cgtgatgggc 14280
gcgcagctcc tctgacactg ccgcgcgcgc gctaccatcc tagcattgcc atttaacaac 14340
tctgcctcct tgcagatatg gccctcactt gccgccttcg tattcctatt gctggctacc 14400
gcggaagaaa gtcgcgccgt agaagagcag ggttgtctgg gagcgggatg cgtcgccacc 14460
ggcggcggcg cgccatcagc aaacggttgg ggggtggatt tcttcccgct ttgattccca 14520
tcatcgccgc ggcgatcggc gcgataccag gcatagcttc cgtggcggtg caggcctcgc 14580
agcgccactg acattggaaa aagatatctt ataaataaaa atagaatgga ctctgacgct 14640
cctggtcctg tgatatgttt ttgtagacga gatggaagac atcaattttt catccctggc 14700
tccgcgacac ggcacgcggc cgtatatggg cacctggagc gacatcggca acagccaact 14760
gaacggggga gccttcaatt ggagcagtct atggagcggg cttaaaaatt ttgggtccac 14820
tataaagact tatgggaaca aagcttggaa cagcagcaca gggcatgcgc tgagacaaaa 14880
gcttaaagat cagaatttcc aacagaaggt ggtcgatggt atcgcctctg gaatcaatgg 14940
ggtggtagat ctggccaacc aggccgtgca gaaacagatt aacagtcgcc tggacccggc 15000
tcccccagct cctattcatg agttaatgca agtggaggaa gagctccctt cattggaaaa 15060
gcggggcgat aagcgacctc gtccagatat ggaggaaacg ctgctgacca aggtggatga 15120
gccgccctcc tatgaagagg ctgtaaaact gggaatgccc actacaaagc ccattatgcc 15180
tctggccact ggagtgatga agccatctca gtctaaacct gcagttgctg ctacattgga 15240
cttgcccgct cccgtggcca cccccaaacc tgtcgccgcc ccgaagccca ccgccgtgca 15300
acccgtggcc gtggccagac cgcgtcccgg tggtcggccg aatgcaaact ggcagagcac 15360
tctgaacagc atcgtgggtt tgggagtgca cagtgtgaag cgccgtcgct gctattgatt 15420
aaatatggag tagcgcttaa cttgcttgtc tgtgtgtgta tatgtcgatg ccgcccgccg 15480
tgctacagca aagagagaag gagaagaggc gccgctgagt tcctttcaag atggccaccc 15540
catcgatgct gccccagtgg gcgtacatgc acatcgccgg acaggacgct tcggagtacc 15600
tgagtccggg tctggtgcag ttcgcccgcg ccacagatac ctacttcaat ctggggaaca 15660
agtttaggaa ccctaccgtg gctcccaccc acgatgtgac caccgaccgt agccagcgcc 15720
tgacgctgcg ctttgtgccc gttgaccggg aggacaatac ctactcctac aaagtcagat 15780
acaccctggc tgtgggagac aacagggtgt tggatatggc cagcacctac tttgacatca 15840
ggggcgtgtt ggacagagga cctagcttca aaccatactc tggcactgcc tacaactccc 15900
tggctccaaa aggagctcca aactccagtc agtggcaaca aaaggaaaac aatggtcaag 15960
gtgatgcaaa gactcacacc tatggtgtag ctgccactgg aggtattgac attgacaaaa 16020
atggtcttca aattggaatc gatgaaacta aagaagataa taacgaaatt tatgcagaca 16080
aaacattcca acctgaacct caaattggag aagaaaactg gcaagatagc gaaaactatt 16140
atggaggcag ggctcttaaa ccggaaacca agatgaagcc ttgctatggt tccttcgcta 16200
gaccaactaa tgcaaaggga ggtcaagcca aaattaaacc agctcaagag ggtcaacagt 16260
ctatagatta tgacatagac ctggctttct ttgatattcc aagcactggc ggaggcaatg 16320
gcacaaatgt aaatgacaag ccagatatgg ttatgtatac tgaaaatgta aatctggaaa 16380
ctccagacac tcatcttgtt tacaagccag gaacttcaga tgacagttcc gaggccaatt 16440
taactcagca agccatggct aacagaccca actatattgg gtttagagat aactttattg 16500
gcgtcatgta ctacaacagc actggcaaca tgggagtgct tgctggtcaa gcatcccagc 16560
taaatgctgt ggtggacctg caagacagaa acaccgagct gtcttatcag ctattacttg 16620
actctctggg cgacagaacc aggtatttta gtatgtggaa tcaggcggtg gacagctatg 16680
atcctgatgt gcgcattatt gaaaaccatg gtgtggaaga tgaattgcca aactattgct 16740
tcccattgga cggagctggc actaatgctg tttaccaagg agttaagaca aaagaggata 16800
ataatggcga atgggaaaca gacacaaatg ttgcatcgca gaatcagata tgcaagggca 16860
acatatatgc tatggagatc aacctgcaag ccaacctgtg gaaaagtttc ctttactcca 16920
acgtggctct gtacctacca gactcctaca agtacactcc atccaacgtg acactcccta 16980
ccaacactaa cacctatgac tacatgaatg gcagggtggt gtctccatcc ctggtggatg 17040
cctacattaa cattggcgcc aggtggtctc tggatgccat ggacaatgtc aaccctttca 17100
accaccaccg caatgccggc ctgcgctacc ggtccatgct tctgggcaac ggccgatacg 17160
tgcccttcca catccaagtg ccccagaaat tcttcgctat caagaacctg ctgcttctcc 17220
caggctcata cacctacgag tggaacttcc gcaaggatgt caacatgatc ctgcagagtt 17280
cccttggcaa tgacctcaga accgatgggg ccaccatcca gtacaccagc atcaatctct 17340
atgccacctt cttccccatg gctcacaaca ctgcctccac cctggaagcc atgctgcgca 17400
atgacaccaa tgaccagtcc ttcaatgact acctctcagc tgccaacatg ctttacccca 17460
tccctgccaa tgccaccaac gtgcccatct ccatcccatc tcgtaactgg gctgccttca 17520
ggggctggtc tttcacccgc ctcaagacca aggagacccc atctctggga tcagggttcg 17580
atccctactt cgtctactca ggctccattc catacctgga tggaactttc taccttaacc 17640
acactttcaa gaaagtctcc atcatgtttg actcttctgt cagctggcca ggcaatgaca 17700
ggctgctgac tcccaatgag ttcgaaatca agcgcactgt tgatggggaa gggtacaatg 17760
tggcacaatg caacatgacc aaagactggt tcctggttca gatgctctcc cactacaaca 17820
ttggctacca gggcttctac atcccagaag gatacaagga ccgcatgtac tccttcttca 17880
gaaacttcca gcccatgagc cgccaggtgg tcgatcaggt caactacaaa gactacatgg 17940
cagtcaccct tgcctatcag cacaacaact ctggctttgt gggctacctc gcgcccacca 18000
tgcgacaggg ccaaccctac cctgctaact acccataccc gctcattggc aagactgcag 18060
tcaacagtgt cacccagaaa aagttcctct gcgacagggt catgtggcgc atccccttct 18120
ccagcaactt catgtccatg ggggccctta ccgacctggg gcaaaacatg ctttatgcca 18180
actccgccca cgcgctagac atgaatttcg aagtagaccc catggatgag tccacccttc 18240
tctatgttgt cttcgaagtc ttcgacgtgg tcagagtgca ccagccccac cgcggcgtca 18300
tcgaagctgt ctacctgcgc acccccttct cagctggtaa cgccaccaca taagcgcctt 18360
gcttcttgca agtggctgca gcagcatggc ctgtggatcc tccactggat ccaatgagca 18420
agagctcagg gccatcgcca tagacctggg ctgtggaccc tatttcctgg gaacctttga 18480
caagcggttt ccaggcttca tggctcctga caagctcgcc tgtgccattg tcaacacggc 18540
agggcgcgag actggtggtg agcactggct ggcttttgga tggaaccccc gctccaatac 18600
ctgctatctc tttgacccgt ttgggttttc agacgagcgc ctcaagcaga tctatcaatt 18660
cgagtacgag gggctcctgc gccgcagtgc cctggctact aaggaccgat gcatcactct 18720
ggaaaagtct acccagaccg tgcagggtcc gcgctcggct gcctgcgggc tcttctgctg 18780
catgttcctc catgcttttg tgcactggcc cgaccgcccc atggacaaca accccaccat 18840
gaatttgctg acgggggtac ccaacaacat gctccaatcg ccccaagtag agcccaccct 18900
gcgccacaac caggaggcac tctatcgctt cctgaactcc cactcatctt actttcgttc 18960
taaccgcgcg cgcattgaga aggccactgc cttcgatcga atgaataata acatgtaaac 19020
caaattgtgt gtggctcaaa taaacagcac tttattgttt acatgcactg aggctctggg 19080
atgatcattt tttaaaaatc gaaggggttc tggcgggaat cagcatggcc agatggcagg 19140
gacacgttgc ggaactggaa cttgttctgc cacttgaact cgggaatcac cagcctggga 19200
actggaatct ctggaaaggt atcttgccat agctttctgg tcagttgcag agcgccaagc 19260
aggtcaggag cagatatctt gaaatcacag ttggggccag aattctgggc gcgggagttg 19320
cggtacactg ggttgcagca ctggaacacc ataagggcag ggtgtctcac gctcgccagc 19380
acggtctcgt cactgatgca agacacatcc aggtcttcag cattggccat tccaaagggg 19440
gtcatcttgc aggtctgtct gcccatcacg ggagcgcagc caggtttgtg gttgcaatca 19500
caatgaaggg ggatcagcat catcttggcc tggtcggggg taatccctgg gtaaacagcc 19560
ttcatgaagg cttcatactg cttgaaagct tcctgggctt tggttccctc ggtgtagaac 19620
actccacaag acttgctgga aaactgatta gtagcgcagt tggcatcatt cacacagcag 19680
cgggcgtcgt tattagccag ctggaccaca ttcctgcccc agcggttctg ggtgatcttg 19740
gctcgatctg ggttctcctt caacgcgcgc tggccgttct cgctcgccac atccatctca 19800
atgacatgtt ccttctggat catgatgttg ccatgcaggc atctaatctt gccttcataa 19860
tcagtgcagc catgaggcca cagcgcgcac ccggtgcact cccaattgtt atgggggatc 19920
tgggaatggc tatgaaccag cccttgcagg aatcttccca tcatcacagc cagggtcttt 19980
atgctggtaa aggtcagcgg gataccgcgg tgctcctcgt tcacatactg ctggcagatg 20040
cgtctgtagt gctcggcctg ctcgggcatc agcttgaaag aggttttcaa ctcattatcc 20100
agcctgtatc tctccatcat gatggacatt acttccatgc ccttctccca ggcagaaaca 20160
atagggagac tcaggggatt cttgacagta gagacaacct tacttaaggg gtcatcactg 20220
ccaatctttt cgatgcttct cttgccatcc ttctcggtga tgcgcaccgg cgggtagctg 20280
aatcccacag ccaccaactg agcctcttcc ctttcgtctt cgctgtcttg actgatgtct 20340
tgcagaggaa catgtttggt tttcctgggt ttcttcttgg gcggcagctc tggaggactc 20400
tggctccgtt ccggagaccc catggatgag cgagagttgt cgctcaccac ttggatctgg 20460
ctgcctgtag aagaactgga ccccacgcgg cggtaggtgt tcctcttggt aggcagaggt 20520
ggaggcgacg ggctccggtc cggtctgggt ggcggatggc tggcggagcc ccttccgcgt 20580
tcgggggtgc gctccagatg gcggtcgtct gactgacctc cgcggctggc cattgtgttc 20640
tcctaggtag agaaacaaga catggagact cagccatcgc tgccatcgcc atccaccacc 20700
acaagcaccg ccgaggagga ggagtgttta accaccccac catgcagccc cgctaccacc 20760
accagcaccc ttgaaagcga ggtcgacacg gtcgtggagg atttacaggc tatggaagat 20820
attgaggcag ctgtcgagca agaccccggc tatgtgacac cggcggagca tgatgaggat 20880
ctagcgcgct ttctcgacgg tgtggagaaa gcgaaacaag atgaggacga ggaagaggca 20940
gaagcacaac catcggtggc cgactacctc accggcctag ggctagaaga cgtgctgctt 21000
aagcatcttg caaggcagac agtcatagtc aaagacgccc tgctagagcg ctccgaggtg 21060
ccactcagtg tggaagacct cagtcgcgcc tatgagctaa acctcttctc gcctcgcaag 21120
ccccccaagc gtcagcccaa cgggacctgt gagcccaatc cgcgcctcaa cttctatcca 21180
gccttcactg tgcccgaagt actagctacc taccacatct ttttcaagaa ccaaaagatc 21240
cccatctcct gccgcgccaa tcgcacccgc gcagatgccc tactcaactt ggggcccggc 21300
gctcgcatac ctgatatcgc ttccttggaa gaggttccta agatctttga gggtctgggc 21360
aatgaggaaa ctcgggcagc aaacgctctg caaagagaaa cagatgatgg tgaacaccac 21420
agcgctctgg tggagctcca gggcgacaac gctcgtcttg cagtcctcaa acgcagcatc 21480
gaggtcaccc atttcgccta ccccgcactt aatctcccac ccaaagtcat gagctcggtc 21540
atggacacgt tgctcatgaa gcgcgcgagc cccatctccg aggatcagaa catgcaggac 21600
cccgatgcct cagatgaagg caagcctgta gtcagcgacg agcaactggc tcgctggcta 21660
ggctctgact ccccccagtc tttggaggag cggcgcaagc ttatgatggc agtggtcctg 21720
atcacagcgg agctggagtg tctccgccgc ttcttcactg acccagagac cctgcgcaag 21780
cttgaggaga acctgcatta cacattcagt catgggttcg tgcgccaggc gtgcaagatc 21840
tccaacgttc aactcaccaa cctggtctcc tacctgggca tcttgcatga aaaccggctg 21900
gggcagaacg tgctccacac caccctgaag ggggaggccc gccgcgacta tatccgcgac 21960
tgtatctacc tctacctatg ctacacctgg caaagcggga tgggtgtgtg gcaacagtgc 22020
ttggaagagc aaaatctaaa agagctggaa aagctgcttc agaaatctct taaatctctg 22080
tggaccgggt tcgatgagcg gaccaccgct tcggacatgg ccgatattat cttccccgag 22140
cggctcagac acactctgcg cgacgggctg cctgactttg ccagccagag catgctacaa 22200
aactttaggt cattcatctt ggaacgctcc gggatcctgc ccgccacttg ctgcgcactg 22260
ccctccgatt ttgtgcccat cacctaccgg gagtgccccc cgccgctatg gagccactgc 22320
tacctgttcc gcctggccaa ctacttggcc taccactctg atgtgataga agatgttagt 22380
ggcgaagggc tcctggagtg ccactgccgc tgcaacctct gcacccccca ccgctccctc 22440
gcctgcaatc cccagctgct gagcgaaacc cagatcatcg gcaccttcga gttgcaaggt 22500
cccagcggcg aaggcgaggg gtcctctccg gggcaaagtc tgaaactgac tccggggcta 22560
tggacctccg cttaccttcg caagttcgcc cccaaagact accaccccta tgagatcagg 22620
ttttatgaag accaatcaca gccccccaag gccgaactga cggcctgcgt catcacccag 22680
ggggcaatct tggcccaatt gcaagccatc caaaaatccc gccaagaatt tttgctgaaa 22740
aagggacacg ggatctatct agacccccag accggtgagg agctgaatac acgcttccct 22800
caggatgccc cgaggaggca agagaatgaa agttcagatg ccgcccgagg aggagctgga 22860
agactgggac agtcaggcag aggaggaaga ctgggacagc caggcagaag aggaggacag 22920
cctggaggag gacagtctgg aggaaggcga ggagcccaag gaagaggcag ccgccgccag 22980
accatcgtcc tcggcggtgg agacaagcaa ggtcccagac agcacggcta ccacctccgc 23040
tccagctcaa ggggccgctc ggcgacccaa cagtagatgg gacgagacgg gtcgcttcca 23100
gaaccccacc accgtcaaga ccggtaagca ggagcggcag ggatacaagt cctggcgggg 23160
gcataaaagt gccatcatcg cttgcttgca ggagtgtggg ggcaatatat cctttgccag 23220
acgctacctg ctattccatc acggggtgaa tttcccccgc aacatcttgc attactaccg 23280
tcacctccac agcccctact accagcagca agagacagca gaggaaacca gcggcaactc 23340
cgagagttag aaaaccagca gctaaaaaat ccacagcggc ggcagcaggt gcaggcggac 23400
tgaggatcac cgcgaacgag ccagctcaga ccagggagtt gaggaatcgg atctttccca 23460
ccctctatgc catattccaa caaagtcggg gtcaggaaca agaactgaaa gtaaaaaaca 23520
gatctcttcg ctcgctcacc cgcagttgtt tgtatcacaa gagcgaagac caacttcagc 23580
gcactctcga ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt 23640
agactgcgcg cgcttggcga gaaaaggcgg gaattacgtc acctcttggc cacacctgtg 23700
cttcattatg agtaaagaaa ttcccacgcc ttacatgtgg agctatcagc cccagatggg 23760
attggccgct ggcgccgccc aggactactc cacccgcatg aattggctca gcgccggtcc 23820
cgcgatgatc tcacgggtta atggtgtgag agagcaccga aaccagatac tcctagaaca 23880
gtccgccctc accgccactc cccgcaatca cctcaacccc cgtaattggc ccgccgccct 23940
ggtgtaccag gaaactcctg ctcccactac agtactactt cctcgtgacg cccaggccga 24000
agttcagatg actaactcag gtgtacagct ggcgggtggt gccaccctgt gtcgtcaccg 24060
gccaagaccg ggtataaagg gcctggtgat cagaggccga ggtattcagc tcaacgacga 24120
gtcggtgaac tcttcgcttg gtctgcgacc agacggcatc ttccaaatag ctggttgtgg 24180
gagatcttcc ttcactcctc gtcaggctgt cctgactttg gagagttcgt cctcgcagcc 24240
ccgctcgggc ggcatcggga ctctccagtt cgtggaggag tttactccct cggtctactt 24300
caaccccttc tccggttctc ctgggcttta cccggacgag ttcatcccga actacgacgc 24360
catcagtgaa gcggtcgacg gctacgatta atgtctaatg gtggcgcggc tgagctagct 24420
cgactgcgac acctagacca ctgccggcgc tttcgctgct ttgctcggga tctctgcgag 24480
ttcatctact tcgagtaccc tgacgaacat cctcagggac ctgcccacgg agttcggatt 24540
accattgaag gggctatcga ttctcacctg cttcggatct tcaccgctcg gccagtgcta 24600
gttgagcgca accagggcga caccaccatc tccctctgct gcatttgtga caaccccgga 24660
ttgcatgaaa gcttttgttg tcttctttgt actgagtata ataaaagctg aaattagaga 24720
ctactccgga ctctcttgtc gtctgaacaa caccaaccag acccttcact tcagcgggaa 24780
ccagactact cttcactgta aggcttataa ctataagtat cttacttgga tatacaaagg 24840
aacaccgttt gctgtggtaa acaggtgctc caacgacggt gttctcctca ccttcctagg 24900
caacttctcc aactttacct tttctgttcg cagaaacaag cttaccctcc ttcagcccta 24960
ctttcctggg atctatacct gcctcagtgg accttgcaac cacacttttc acctgattga 25020
aaactctacc cttaccttcc cagcgccaat ccctactaac agctcggagt ccaactcttc 25080
cattaccgct gatactaaca ctcctaaaac cggaggtgag ctccgcagcc ttcccccggc 25140
tgcagataac ccttgggtgg tagcgggatt tgtagcgcta ggaatagttg cgggtgggct 25200
cgcgttcgtc ctctgctacc tataccttac ctgctgctca tatttagtag tactgtgctg 25260
ttggtttaga aaatgggggc gctactaatc acacttgctt tactttcgct tttgggtctg 25320
agctcggcta atagcgagaa accaagctgt ctagaaacaa actctccaga ctgtgtggtt 25380
cctcatgggc tctcagaccc agctgatgat ccatgcttaa cttttgaccc agaaaaaaac 25440
tgctcggtga ctatgcagcc ctatgcttac atgtgcacat ctgttataaa gtgcggatgg 25500
ggctgtaaac cgattgaaat tacccacaaa ggcaaaacct ggaataatag tttgtttaac 25560
acatggcagc ctggagacga gcagtggtat acggccggcc actggtggag atgactgacc 25620
ccatggaaaa ctcctctgcc aacgacctgg acatggacgg ccgttcatct gagcagcgac 25680
tggtccagat gcgcattcgc cagaagcagg aacgcgccgc cagagagctc aaggatgcca 25740
ttgaaattca cctgtgcaag aagggcatct tttgcttggt taagcaagca aagatttctt 25800
atgaaatcac tgacaacgac caccgcctgt attatgagct cggtccacag cggcagaaat 25860
tcacctgcat ggttggagtc aaccccatag tcatcactca gcaggctgca gaaattaaag 25920
ggtgcatcca ctgttcctgt gattcccaag aatgcgtcca caccatagtc aagaccctct 25980
gcggccttcg agatcttctt ccaatgaact aaccccttcc cccaacccaa taaaacattg 26040
gttttaatca taataaaaaa tcacttactt taaatctgaa acagtgtctc cgtccaagtt 26100
ttcttgtagc accacttcac tcccctcttc ccagctctgg tactgcaagc cccggtgggc 26160
tgcaaacttt ctccacacct taaaagggat gtcaaattcc tcttgtccaa caatcttcat 26220
tgtctcttcc tagatgtcca caaagcgcgc gcgggtggaa gatgactttg accctgtcta 26280
cccatacgat gctgagctgg caccgtctgt acccttcatc gcccctccct tcgtttcgtc 26340
agacggattt caagaaaaac ccctgggagt tctgtcccta agactagcca acccagtcac 26400
tactaaaaat ggggaactca cacttaaact gggagatggg gtgggcatag actcagatgg 26460
aaacctcaca gcacagacag ttactaaagc aacatccccc cttactgttt ccaataacgc 26520
aattgcactt aacatggaca aaccttttta cagtagcaat ggaaaactat ccttacaagt 26580
tacatcacca ttaaagatag tcgactcttt aaatacattg gctattggct atgggcaagg 26640
cttaggacta aacaatagtg ctcttgctgt gcaattagca tctcccctta catttgacag 26700
caacagcaaa attaaaataa atttgggaag cgggccatta aaaattaatg cgaataaact 26760
gtcaattaac tgcctaaggg gtgtatatgt aacaactgac ggaacttcca ttgaaacaaa 26820
tataagctgg gcaaaaggaa tgaggtttga aggtaatgcc atggctgtaa acgttgacag 26880
caccaaaggt ctacaatttg gcactaccag cacagaatca ggagtcacta acgctttccc 26940
tatccagtta aagattggat ctggtcttag ttttgacagc acaggagcac ttgtagcttg 27000
ggataaggat aatgacaagc ttacactgtg gacaaccgct gacccatcac ctaattgtac 27060
catatataca gacaaggatg ctaaacttac actttgtctt acaaaatgtg gcagtcaaat 27120
actaggcagt gtttcagtac tggctgttaa agctggaacc ctacagccaa tcagtgaaaa 27180
aataggtact gctttggttt cactaaaatt taataacaac ggtgtattgt taagcaactc 27240
cacattaagt aatgaatact ggaactacag gaagggagat gtcacaccag ccgaagccta 27300
tactaatgct gtgggtttta tgccaaacat caaggcatat cctaaaaaca caaactctgc 27360
ctcaaaaagc cacattgtag gacaagtgta ccttaatgga gatgaaacta aaccaatgca 27420
tttaatcatt acatttaatg aaaccagtga tgaaacatgc acatattcca taacgttcca 27480
atggaaatgg aacattggaa catacaccag cgacaccctt gcaacaagct cctttacctt 27540
ttcttacatt gcccaagaat aaaaactgca gacaacaata aagtttaaat gttttattta 27600
aacagtttca cagaacccta gtattcaacc tgccacctcc ctcccaacac acagagtaca 27660
cagtcctttc tccccggctg gccttaaaaa gcatcatatc atgggtaaca gacatattct 27720
taggtgttat attccacacg gtttcctgtc gagccaaacg ctcatcagtg atattaataa 27780
actccccggg cagctcactt aagttcatgt cgctgtccag ctgctgagcc acaggctgct 27840
gtccaacttg cggttgctta acgggcggcg aaggagaagt ccacgcctac atgggggtag 27900
agtcataatc gtgcatcagg atagggcggt ggtgctgcag cagcgcgcga ataaactgct 27960
gccgccgccg ctccgtcctg caggaataca acatggcagt ggtctcctca gcgatgattc 28020
gcaccgcccg cagcataagg cgccttgtcc tccgggcaca gcagcgcacc ctgatctcac 28080
ttaaatcagc acagtaactg cagcacagca ccacaatatt gttcaaaatc ccacagtgca 28140
aggcgctgta tccaaagctc atggcgggga ccacagaacc cacgtggcca tcataccaca 28200
agcgcaggta gattaagtgg cgacccctca taaacacgct ggacataaac attacctctt 28260
ttggcatgtt gtaattcacc acctcccggt accatataaa cctctgatta aacatggcgc 28320
catccaccac catcctaaac cagctggcca aaacctgccc gccggctata cactgcaggg 28380
aaccgggact ggaacaatga cagtggagag cccaggactc gtaaccatgg atcatcatgc 28440
tcgtcatgat atcaatgttg gcacaacaca ggcacacgtg catacacttc ctcaggatta 28500
caagctcctc ccgcgttaga accatatccc agggaacaac ccattcctga atcagcgtaa 28560
atcccacact gcagggaaga cctcgcacgt aactcacgtt gtgcattgtc aaagtgttac 28620
attcgggcag cagcggatga tcctccagta tggtagcgcg ggtttctgtc tcaaaaggag 28680
gtagacgatc cctactgtac ggagtgcgcc gagacaaccg agatcgtgtt ggtcgtagtg 28740
tcatgccaaa tggaacgccg gacgtagtca ttctcgtact ttgagtggca aaaccttgct 28800
ctcgaacagc acacgtctcg tcgcctcctg tcccttctct tcgccttttc agtgtgatag 28860
ttgtaataca gccattcacg aagctcagtc agaagatctt cagcgtctgt tgtcaaaaac 28920
aatccatcca atctgattgc tttcaaaaca tcacaaacag tcgaataagc caaacccatc 28980
caggcaatgc aattattttg gttatccaca atgggagggg gcggaagaca tggaagaggc 29040
ataattaatt ttttaatcca atcgatcacg cagcacttca aaatgaagat cgcgaaggtg 29100
acacctttca cccccactgt gttgatgaaa aataacagcc aagtcaaaat tgatgcggtt 29160
ttcaaggtgc tcgactgtag catcaagcag agcttccaca cgcacgtcca caaataacag 29220
aatagcaaaa gcgggaggag gaagtaaatc ctcaatcatc atagtacagt ccatcaccat 29280
ccctaaataa ttttcatcct tccagccttg gactatattt ttaaactgct cttgtaaatc 29340
caaaccacac atgtggaaaa gttcccaaag agctccctca actaccattc ttaagcacac 29400
cttcatagtg acaaaatatc ttgttcctct gtcacctgca gcaaattaca aagtccaata 29460
ttaggatcta tgcccagaga tctaagctca tccctcaatt ccaactgtaa aaaggcttcc 29520
agatctgccc taacttgttc agccagtggg ctccctggaa taagcgtggg agaagccaaa 29580
ctgcaaaaca gacgcatgcc gccataatta ccaccagaaa acactacgtt acagtatgca 29640
tgctgattca ttccagtaat ttcatccagt gtattggata caaaaaaagg caagcactct 29700
ctcactaatt gtattatgga gacattatca cacaggtaac aatttaaagg ttgtggaaca 29760
ataatgcagt aagtaaccac ggtgcgctcc aacatggtta gtaattttta gttctgaaaa 29820
acaaaacata caaaaaatta tatcatactc atttggcgaa ctggtggaaa aatgacccta 29880
tctagcacaa ggcaagccac tggatcacca atgcgcccct cataaaacct gtcatcatga 29940
ttaaaaagca acaccgaaag ctcttcccta tgtcctgcat gaatgattct agctgaggaa 30000
tataagccag cgcaattagt atctgttaaa gaaaaaaaac ggccaacata gcctctagga 30060
attagcacac ttaatcttaa agacattact gccatccccc ttggatttaa ggtaaaattt 30120
acaggagcat agaaaatata ctgatttccc tcctgcacag gcagcatagc accaggtccc 30180
tctaaaaaca cacacaaagc ttctgcagcc atagcttacc gcgcaaacca ggcacagcag 30240
tgagctaaaa ggacaaagct ctaactcact agccaacctg gcgcacaata tatagttagt 30300
ccttacactg acgtaaccga ccaaagtcta aaaaccccgc caaaaataca cacacgccca 30360
aaaaacgccc cgtgagtcaa aaaacagttt cacttcctcg ttacacccaa aacgtcgtca 30420
cttccggatt cccacggttc gtcacttccg gagctccttg cttaattaac cccgcccaaa 30480
acgtcatcgt ccgcgtcacg ccgccccgcc ccgcgaccgt tgaccccggg ccaatcaccg 30540
cacatcccgc aaaattcaaa ctcgtctaat ttgcatattg gcacactgcc catataaggt 30600
atattattga tgatgattta aatcatatgc ggtgtgaaat accgcacaga tgcgtaagga 30660
gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 30720
ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 30780
caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 30840
aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 30900
atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 30960
cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 31020
ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 31080
gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 31140
accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 31200
cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 31260
cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 31320
gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 31380
aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 31440
aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 31500
actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 31560
taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 31620
gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 31680
tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 31740
ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 31800
accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 31860
agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 31920
acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 31980
tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 32040
cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 32100
tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 32160
ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 32220
gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc 32280
tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 32340
ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 32400
gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 32460
cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 32520
gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 32580
ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga 32640
cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa ttgatttaaa 32700
t 32701
<210> 10
<211> 179
<212> PRT
<213> 人工序列
<220>
<223> BLY6纤维突结
<400> 10
Thr Leu Trp Thr Thr Ala Asp Pro Ser Pro Asn Cys Thr Ile Tyr Thr
1 5 10 15
Asp Lys Asp Ala Lys Leu Thr Leu Cys Leu Thr Lys Cys Gly Ser Gln
20 25 30
Ile Leu Gly Ser Val Ser Val Leu Ala Val Lys Ala Gly Thr Leu Gln
35 40 45
Pro Ile Ser Glu Lys Ile Gly Thr Ala Leu Val Ser Leu Lys Phe Asn
50 55 60
Asn Asn Gly Val Leu Leu Ser Asn Ser Thr Leu Ser Asn Glu Tyr Trp
65 70 75 80
Asn Tyr Arg Lys Gly Asp Val Thr Pro Ala Glu Ala Tyr Thr Asn Ala
85 90 95
Val Gly Phe Met Pro Asn Ile Lys Ala Tyr Pro Lys Asn Thr Asn Ser
100 105 110
Ala Ser Lys Ser His Ile Val Gly Gln Val Tyr Leu Asn Gly Asp Glu
115 120 125
Thr Lys Pro Met His Leu Ile Ile Thr Phe Asn Glu Thr Ser Asp Glu
130 135 140
Thr Cys Thr Tyr Ser Ile Thr Phe Gln Trp Lys Trp Asn Ile Gly Thr
145 150 155 160
Tyr Thr Ser Asp Thr Leu Ala Thr Ser Ser Phe Thr Phe Ser Tyr Ile
165 170 175
Ala Gln Glu
<210> 11
<211> 219
<212> PRT
<213> 人工序列
<220>
<223> BLY6纤维轴
<400> 11
Gly Val Leu Ser Leu Arg Leu Ala Asn Pro Val Thr Thr Lys Asn Gly
1 5 10 15
Glu Leu Thr Leu Lys Leu Gly Asp Gly Val Gly Ile Asp Ser Asp Gly
20 25 30
Asn Leu Thr Ala Gln Thr Val Thr Lys Ala Thr Ser Pro Leu Thr Val
35 40 45
Ser Asn Asn Ala Ile Ala Leu Asn Met Asp Lys Pro Phe Tyr Ser Ser
50 55 60
Asn Gly Lys Leu Ser Leu Gln Val Thr Ser Pro Leu Lys Ile Val Asp
65 70 75 80
Ser Leu Asn Thr Leu Ala Ile Gly Tyr Gly Gln Gly Leu Gly Leu Asn
85 90 95
Asn Ser Ala Leu Ala Val Gln Leu Ala Ser Pro Leu Thr Phe Asp Ser
100 105 110
Asn Ser Lys Ile Lys Ile Asn Leu Gly Ser Gly Pro Leu Lys Ile Asn
115 120 125
Ala Asn Lys Leu Ser Ile Asn Cys Leu Arg Gly Val Tyr Val Thr Thr
130 135 140
Asp Gly Thr Ser Ile Glu Thr Asn Ile Ser Trp Ala Lys Gly Met Arg
145 150 155 160
Phe Glu Gly Asn Ala Met Ala Val Asn Val Asp Ser Thr Lys Gly Leu
165 170 175
Gln Phe Gly Thr Thr Ser Thr Glu Ser Gly Val Thr Asn Ala Phe Pro
180 185 190
Ile Gln Leu Lys Ile Gly Ser Gly Leu Ser Phe Asp Ser Thr Gly Ala
195 200 205
Leu Val Ala Trp Asp Lys Asp Asn Asp Lys Leu
210 215
<210> 12
<211> 44
<212> PRT
<213> 人工序列
<220>
<223> BLY6纤维尾
<400> 12
Met Ser Thr Lys Arg Ala Arg Val Glu Asp Asp Phe Asp Pro Val Tyr
1 5 10 15
Pro Tyr Asp Ala Glu Leu Ala Pro Ser Val Pro Phe Ile Ala Pro Pro
20 25 30
Phe Val Ser Ser Asp Gly Phe Gln Glu Lys Pro Leu
35 40
<210> 13
<211> 30954
<212> DNA
<213> 人工序列
<220>
<223> BLY6.dE1.dE3腺病毒载体基因组
<400> 13
catcatcaat aatatacctt atatgggcag tgtgccaata tgcaaattag acgagtttga 60
attttgcggg atgtgcggtg attggcccgg ggtcaacggt cgcggggcgg ggcggcgtga 120
cgcggacgat gacgttttgg ggaggaggag ctatgttgca agtaatcgtg ggaaatgcga 180
cgtaaaacga ggtggagttt aaacacggaa gtagacaatt ttcccgcgct gtttgacagg 240
aaatgatgtg tttttgggcg gatgcaagtg aaaattctcc attttcgcgc gaaaactaaa 300
tgaggaagtg aaattctgag taattctgag gttatcacag ggcggagtat ttaccgaggg 360
ccgagtagac tttgacccat tacgtggagg tttcgattac tctatttttc acctaaattt 420
ccgcgtactg tgtcaaagtc cgtgttttta cgcgatcgcg atggcctgtg tttgagcata 480
atgtactgac caggtgtaac gttcatctgg gtggtcgtag aggaatgttc atgccatacc 540
aatgcaattt taatcatgtg aggatcttga tggagccgca agcgttttcc agagtcagct 600
tgactggaat ctttgacatg tgtgtggaag catggaagat cttaagatat gatgatacca 660
aatccagatg ccgcgcatgc gagtgcgggg gcaggcatgc caggttccaa cctgtatgtg 720
tggaggtgac cgaggagctg agaccagatc atttggtgct gacctgcact ggtgcggagt 780
tcggttccag tggtgaagaa actgattaaa gtgagtagtg ggatgttata aaagtgacca 840
taaggtgatg tgagatggac aaatttggta atttttatgt atttttgtct tgcagccatg 900
agtgggagcg cttcctttga agggggcgtc tttagccctt atctgacggg gcgtctgcct 960
cattgggctg gagtgcgtca gaatgtgatg gggtctacag tggatggaag acctgttcag 1020
cctgctaatt cttctactct gacttatgct actatgactt cctcgccttt ggatgcagct 1080
gcagctgctg ccgcttctgc tgccgccaac actgttcggg ggatggcctt ggagatgggg 1140
tattatggaa ctgtagtggc caacaccact accccaaata accccacagc cttgaatgag 1200
gacaagctgc tagttctcat gtcccagctg gagtctttga cccaacgcct gggcgatcta 1260
gctcagcagg tgtcccagct gaaggagcag actcaagctg caattaccac tgcgagggga 1320
aattaaaaaa attcaaagaa tcaataaata aaccgagact ttgttgattt taaagtgtgt 1380
cattctttat ttaatttttc gcgcgcgata tgccctggac caccggtctc tatcattgag 1440
gacacggtgg atcttttcta gaacccgata gaggtgggat tggatgttga ggtacatggg 1500
cataagacca tctttggggt gtagatagct ccactgcaga gcctcatgct ccggggtggt 1560
gttgtatata acccagtcat agcatgggcg ttgggcatga tgttgcacaa tatctttaag 1620
gaggagacta atggccactg ggagaccctt ggtgtaagtg tttacaaatc tattaagctg 1680
ggacgggtgc atccgaggtg agataatgtg cattttggat tggattttta gattggcaat 1740
gtttccccct agatctctcc tgggattcat gttatgcaag accactagaa cagtgtatcc 1800
ggtgcactta gggaatttgt catgaagttt ggaggggaaa gcatgaaaaa atttagacac 1860
acccttgtgt cctcccaagt tctccatgca ctcatccata ataatggcaa tgggcccatg 1920
ggcggcggca cgggcgaaca cgttcctggg atctgacaca tcatagttgt ggtcttgggt 1980
caggtcatca taagccattt taataaactt ggggcggagg gtgccagatt gggggatgaa 2040
tgttccctcg ggccccggaa catagtttcc ttcacatatt tgcatttccc aggcttttag 2100
ttcagagggg gggatcatgt ccacctgtgg agcgatgaag aagacggtct cgggggcggg 2160
ggtgattaag tgggaggaca gcaagttcct aagcagctgt gacttgccac acccagtggg 2220
accgtagatg acccctataa caggttgcag atggtagttt agggaaagac agctgccgtc 2280
ctctcgcagg aggggggcga cctcgttcat catttccctc acatgcatgt tttcccgcac 2340
aagttccgat aggaggcgct ctccacccag ggaaaggagt tcttgaagag atgagaaatt 2400
tttcaagggt tttaagccat cagccatggg cattttggag agggtttgtt gcaagagttc 2460
aaggcggtcc cagagttcgg tgatgtgttc tatggcatct cgatccagca tacttcctcg 2520
tttctggggt tgggacggct gcgggagtat ggaaccaggc gatgggcgtc cagcgctgcc 2580
agtgtccggt ccttccacgg tcgcagcgtc cgagtcaggg tcgtttccgt cacggtgaag 2640
gggtgcgcgc ctggctgggc gcttgcgagg gtgcgcttca ggctcatcct gctcgtggag 2700
aaccgctgcc gttctgcgcc ctgtgcatcg gccaggtagc aattaaccat gagttcgtag 2760
ttgagcgcct ctgccgcgtg gcctttggcg cgcagcttac ctttggaagt cttctgacag 2820
gtgggacagt agagacactt gagagcatag agttttgggg ctagaaagac cgattctggg 2880
gagtatgcat cggccccaca ggaggcgcag acggtttcgc attccaccag ccatgtaaga 2940
tcgggctcgt tggggtcaaa aacaagtttt ccgccatgtt ttttgatgcg tttcttacct 3000
ttgctttcca tgagttcgtg cccccgttgg gtgacaaaga ggctgtccgt gtccccgtag 3060
actgacttta tgggcctgtc ctcgagcggc gtgccgcggt cctcttcgta gaggaactcg 3120
gaccactctg agacgaaagc acgtgtccag gccagcacaa aggaggctat atgggagggg 3180
tagcgatcgt tgtcaaccaa ggggtctact ttttccaagg tgtgtaaaca catgtcccct 3240
tcttccacat ccaggaaggt gattggcttg taagtgtatg ccacgtgacc tggggtccca 3300
gacggggggg tataaaaggg ggcgggtctc tgctcgtcct cactgtcttc cggatcgctg 3360
tccaggagcg ccagctgttg aggtaggtat tccctctcga aggcgggcat aacctccgca 3420
ctcaggttgt cagtttctag gaacgaggag gatttgatat tgacagtgcc tgccgagatg 3480
cctttcatga gactgtcgtc catttggtca gaaaagacaa tctttttgtt atcaagtttg 3540
gtggcgaagg atccatacag ggcattggaa agcagtttgg caatggagcg catggtttgg 3600
tttttttctt tgtctgcgcg ctctttggcg gctatgttga gttggacata ttcgcgggcc 3660
agacatttcc attgtggaaa tatggtagtt aattcatctg ggacgattct gactttccag 3720
cctctgttat gcagggtaat cagatccaca ctggttgcca cttctcctct aagtggttca 3780
ttagtccagc atagtcgccc cccttttcga gaacagaaag ggggtagggg atctagcatg 3840
agttcgtctg gggggtctgc atctatggtg aaaatcccag gaaggagatc ttcgtcaaaa 3900
tagctgatgg tggcggggtc atccagagac atttgccatt ctcgagcagc cagagcgcgc 3960
tcgtaggggt taaggggagt cccccatggc atgggatggg tgagtgcaga agcatacatg 4020
ccacagatgt catagacata gagcggctct tccagaatcc ctatgtaagt gggataacat 4080
cgcccccctc tgatgctggc tcgcacataa tcatagagtt catgtgaggg cgctagaaga 4140
cccgagccca ggttggtgcg gttgggtttt tctgctctgt agaggatctg gcgaaagatg 4200
gcatgggagt ttgatgagat ggtgggtctt tggaagatgt tgaaatgggc atgaggcagt 4260
cccacagagt cccttatgaa gtgagcatag gagtcttgca gtttggccac cagctcggcg 4320
gtgaccagca catccaaagc acagtagtcg agggtctctt tgatgatgtc atagttaggt 4380
tcccctttct tttcccacag ctcgcggttg agaaggtatt cttcgcgatc cttccagtac 4440
tcttcgaggg ggaacccgtc cttgtctgaa cggtaagaac ccagcatgta aaattgattg 4500
acagctttgt aggcacaaca ccccttctcc acggggagtg agtatgcttg cgcggctttg 4560
cgcagagagg tgtgagtaag ggcgaaagtg tccctgacca tgactttgag gaactgatgc 4620
ttaaagtcta tgtcatcgca ggccccctgc tcccacagtt ggaagtccac tcgctttttg 4680
taggcgggat tgggcaaagc gaaagtaaca tcgttgaata ggatctttcc agccctgggc 4740
atgaagttgc gagtaatgcg aaaaggctga ggcacttctg ccctgttgtt gataacttgg 4800
gcagccaaga cgatctcgtc aaagccgttg atgttgtgac ccacaatgta aagttctacg 4860
aagcgtgggc gtcccttgat gtggggcagt tttttaagct cttcgtaggt caagtcgtca 4920
gggtcagcga ttccatattg ctccaaagcc cagtcaggca ggtgaggatt agcatgaagg 4980
aaagaggtcc aaagatccac ggccagagct gtttgtaagc ggtctctgta ctgacggaaa 5040
tgtcggccta ccgccatttt ttcaggagta acacagtaaa aggtgcgcgg gtccttttcc 5100
cagcgatccc attgaagttg caaggctagg tcgtgggcga ggttgacgag ctgttcgtcc 5160
cccgaaagtt tcatgaccag catgaaaggg acaagctgct tgccaaagga ccccatccag 5220
gtgtaggttt ccacatcgta ggtgaggaag agcctttctg tgcgaggatg agaaccgatc 5280
gggaagaact ggatttcctg ccaccagttg gaggaatggc tgttgatatg atggaagtag 5340
aactccctac ggcgcgccga gcattcgtgc ttgtgcttgt acagacggcc acagtactcg 5400
cagcgctgca cgggatgcac ctgatgaatg agctgtacct ggcttccttt gacaagaaat 5460
ttcagtggga agttgaggcg tggcgtctgc atctcgtgtt gtattacgtc ctggctattg 5520
gtctggccat cttctgtctc gatggtggtc atgctgacga gcccgcgcgg gaggcaggtc 5580
cagacctccg cgcggacggg tctgagagcg aggacgagag cgcgcaggcc ggaactgtcc 5640
agggtcctga gacgctgcgg agtcaggtca gtagggagag tacataggtt tacttgcata 5700
agtttttcca gggcatgtgg gaggtcaaga tgatatttga tttctactgg cgagttggtg 5760
gagacatcga tggcttgcag ggtcccgtgc ccctggggtg ctaccaccgt cccttttttt 5820
ttcttgatcg ggggcggtgt tgcttcttgc atggtaaggt cgtcttctag aagcggcggc 5880
gaggtcgcgc gccgggtggc agtggcggtt ctggacctgg aggtagaggc ggtagaggta 5940
cgtcggcgcc gcgcgcgggt aggttctggt actgcgccct gagaagactt gcgtgagcga 6000
cgacgcggcg gttgacgtcc tggatctgac gcctctgggt gaatgctacc ggacccgtga 6060
gcttgaacct gaaagagagt tcaacagaat caatttcggt atcgttgacg gctgcctgcc 6120
gcaggatttc ttgtacgtcg cccgagttgt cttggtaggc gatctcggcc atgaactgct 6180
cgatctcttc ttcttggaga tctccgcggc ccgctcgttc tacggtggca gcaaggtcgt 6240
tggagatgcg ccccatgagc tgtgagaatg cattcatgcc cgcctcgttc cagacgcgac 6300
tgtagaccac ggctccctcg ggatctctgg cgcgcatgac cacttgggcg aggtttagtt 6360
ccacgtgtct ggtgaagacc gcatagttgc agagacgctg gaagaggtag ttgagcgtgg 6420
tggcgatgtg ctcggtgaca aagaaataca tgatccagcg acgaagcggc atctcgctga 6480
tatcgcccag ggcttccaac cgttccatgg cttcgtaaaa gtccacggcg aagttgaaaa 6540
actgggagtt gcgagcggac acggtcaact cctcctccag aagacggatg agctcggcga 6600
tggtggcgcg cacttcgcgc tcaaaggctc ccgggatctc ttcctcctct tcttcttcca 6660
actcttcctc cactaacatc tcttctactt cctcctcagg cggcgggggt ggaggagggg 6720
gcgcgcggcg acgccggcga cgcacgggca gacgatcgat gaagcgttcg atcacttctc 6780
cgcggcggcg acgcatggtc tcggtgacgg cgcgcccgtc ctccctgggt cgcagagtga 6840
agacgccgcc gcgcagctcc ctgaaatggt gactgggagg gtccccgttt ggtagggaca 6900
gggcactgat gatgcatctt attaattgcc ctgtagggac tccgcgcaag gacctgagcg 6960
tctcgagatc cacgggatct gaaaatctct gaacgaaggc ttcgagccag tcgcagtcgc 7020
aaggtaggct gagcactgtt tcttcggggc gggctgctga gctagagggt tgtacgatgc 7080
tgctggtgat gaagttaaaa taggcagttc tgagacggcg gatggtggcg aggagcacca 7140
ggtctttggg tccggcttgc tggatgcgca ggcggtcggc cattccccat gcattatctt 7200
ggcacctggc cagatcttta tagtagtctt gcatgagtcg ctccacgggc acttcttctt 7260
cgcccgctct gccgtgcatg cgtgtgagtc cgtaccctct ctgtggttgg acgagcgcca 7320
ggtcggcaac gaccctttcg gctagaatgg cttgctgcac ctgggtgagg gtgttctgga 7380
aatcatcaaa gtccacaaag cggtggtagg cccccgtgtt gatggtgtag gagcagttgg 7440
acatgaccga ccagttgact gtctggtgtc ctggtcgtac gagttccgtg tacctgagcc 7500
gcgagtatgc gcgggagtcg aagatgtaat cgttgcaggt tcgcaccagg tactggtagc 7560
cgatgaggaa gtgaggcggc ggctggcggt agagaggcca tcgttcggtg gcgggcgcgc 7620
cgggcgctag gtcttctagc atgagacggt ggtatccgta gacgtacctg gacatccagg 7680
taataccggc ggcggtggtg gaggcgcgcg gaaactctcg cacgcggttc cagatgttgc 7740
gcagcggcat gaagtagttc atggtgggca cggtctggcc cgtgaggcgc gcgcagtcat 7800
tgatgctcta gatacgggca aaaacgaaag cgttgagcgg ttcccttccg tggcctggag 7860
gaacgcgaac gggttaggtc gcagcgtacc ctggttcgag actaaagaaa gcgagcaact 7920
cgaaccggca gagtcgcggc taacgggtat tggcaatccc gtctcgaccc aagccagcaa 7980
atccaggata cggatggggg ccccttttgt ttttcagggc atgagtcacc ggttaaggtt 8040
tacaacggct gtttcatgcc tttagaagtg gctcgcgccc gtagtctgga gaatcaatcg 8100
ccagggttgc gttgcggcgt gccccggttc gagcctgcag cttgagtcgg ccggtgaccg 8160
cggcaaacga gggcgtggcg gccccgtcgt ttctaagacc ttgctagccg acctctccag 8220
tttacgggaa cgagccccct tttatttttt ttgtttttgc cagatgcatc ccgtactgcg 8280
gcagatgcgc ccacagcccc cacagcagca gcagcaggct ggcctacctt ctctacctca 8340
gccgctacct gcaactaccg cggtggccgc tgtaagcggg gccggacagc aggcggctcc 8400
tcaatatgaa ttggacttgg aagagggcga gggattggca agattggggg cgccctcgcc 8460
cgagcgccac ccgcgggtgc agatgaaaaa ggacgttcgc gaatcttacg tgcccaagca 8520
gaatctgttc agagacagaa gcggcgagga gcccgaggag atgcgcgcgt cccgttttaa 8580
cgcgggtcgc gagctgcgac aaggactgga tcgaaaacgg gtgttgaggg atgatgattt 8640
tgaggtggat gaaatgacag ggatcagccc cgctcgcgct cacgtggctg cagctaatct 8700
ggtgacagct tatgagcaga ccgtgaagga ggaaagcaac ttccagaaat cattcaataa 8760
ccacgtgcgc accctgatcg cacgcgagga ggtgaccctg ggcctgatgc acctgtggga 8820
tctgctggaa gccatagtgc agaaccccac tagcaaaccc ctgactgctc aactgtttct 8880
ggtggtgcag cacagcaggg ataatgaggc attcagagag gcgctgctga atatcactga 8940
acctgagggg agatggctgc tggatctggt gaatatcctg cagagcattg tagtgcagga 9000
acgcagcttg cctttgtccg agaaggtggc ggcgatcaat tactctgtgc tgagtctggg 9060
caaatactat gccaggaaga tctacaaaac cccttacgtg cccatagaca aggaagtgaa 9120
aatagatggg ttttacatgc gcatgaccct gaaagtgcta accctgagcg atgacttggg 9180
agtgtaccgc aacgacagga tgcaccgcgc ggtgagcgcc agcaggaggc gcgagctgag 9240
cgacaaagaa ttaatgcaca gcttgcaacg agccctgacg ggagccggga cggaggggga 9300
gaactacttt gacatgggtg cagacttgca ttggctgcct agtcgcaggg cattggaagc 9360
ggcaggcgat gggccctatg tagaggaagt agtagacgag gacgatgagg agggcgagta 9420
cctggaagac tgatggcgcg acccgtattt ttgctagatg gaacaggcgc cggaccctgc 9480
gatgcgggcg gcgctgcaga gccagccgtc cggcattaat tcctcggacg attggaccca 9540
ggccatgcaa cgcatcatgg cgctgacgac ccgcaacccc gaagccttta gacagcagcc 9600
tcaggccaac cgcctttcgg ccatcctgga ggccgtggtg ccctctcgct ccaaccccac 9660
ccacgagaag gttctggcca tcgtgaatgc cctggtggag aacaaggcca tccgctccga 9720
tgaagccggg ctggtataca acgccttgct cgagcgcgtg gctcgctaca acagcagcaa 9780
tgtccagact aacctggaca ggatggtgac cgacgtgcgc gaggccgtgt cccagcgcga 9840
acggttccat cgcgagtcta acctgggttc catggtagcg ctgaacgctt tcctcagttc 9900
ccagcctgcc aatgtgcccc ggggacagga agactatacc aactttatta gcgccctgag 9960
actcatggta gccgaggttc ctcagagcga ggtgtaccag tccggtccag actacttttt 10020
ccagacaagc aggaacggta tgcagacagt gaacttaagc caggctttca agaacctgca 10080
agggctgtgg ggagtccaag ctccagtggg cgacagggcg accgtgtcga gcctgttgac 10140
tccaaattcc cgtttgctgc tgctgctggt gtcccccttc actgacagcg gcagcataaa 10200
cagaaactcc tacttgggct acctgataaa cttgtatcgc gaagctatag gtcaggccca 10260
cgtggacgaa cagacctatc aggagatcac taatgtgagt cgcgctctgg gccaggacga 10320
ccctggaaac ctggaagcta ctctaaactt tctgctgacc aaccgctcgc aaaaaatccc 10380
tcctcagtat acattaactg cggaggagga acggatcttg agatacgtgc agcagagcgt 10440
gggtctgttc ctgatgcaag agggtgcgac ccctagcgcc gcgcttgata tgacagcgcg 10500
caacatggag cccagcatgt atgccagcaa cagaccattc attaataaat tgatggatta 10560
cttccatcgc gcggccgcta tgaactctga ttacttcacc aatgctattc tgaaccccca 10620
ttggctgcct ccgcctggtt tttatactgg cgagtatgac atgcctgacc ccaacgatgg 10680
gttcttgtgg gacgatgtgg acagcgtggc gttctcgcct accgctcctc gtactttttg 10740
gaagaaggaa ggtagtgaca gaagaccctc ctccgtgctg tcaggacgtg agggtgctgc 10800
cgcggcggtc cccgatgctg caagcccctt tcccagtctg ccattttcac taaacagcgt 10860
gcgcagtagc gagctgggga gaataacccg ccctcgcttg ctgggcgagg acgagtattt 10920
gaatgactcc ctactgagac ccgagcggga aaagaacttc cctaataatg ggattgaaag 10980
cctggtggat aagatgagca gatggaagac ctatgcccag gagcacagag atgagcctag 11040
aatcttgggt cctacagtag gcacccgcag acgccagcgc catgatagac agcggggtct 11100
ggtgtgggac gatgaggatt ctgcagatga cagcagcgtg ttggacttgg gcgggagggg 11160
aggtgtgggc aacccgttcg cacacttgcg tccccgtatt ggacgcatga tgtaaaagtg 11220
aaaataaaaa aggaactcac caaggccatg gcgaccagcg tgcgttcgtt ctttctgttg 11280
ttgtatctag tatgatgagg cgcaccgtgc taggcggatc ggtggcgtat ccggagggtc 11340
ctcctccttc gtacgaaagc gtgatgcagc aggtggcggc ggcggcgatg caacccccct 11400
tggaggctcc ttacgtgccc ccgcggtacc tggcacctac cgaggggaga aacagcattc 11460
gttattcgga actcacaccc ttgtatgaca ccacccggtt gtacctggtg gacaacaaat 11520
cggcggacat tgcctcgttg aactatcaga acgaccacag caacttcttg acaacggtgg 11580
tgcagaacaa tgactttacc cccacggagg ccagcaccca gaccatcaac tttgacgagc 11640
gctcccggtg gggcggtcag ctgaagacca tcatgcacac caacatgccc aacgtgaacg 11700
agttcatgtt tagcaacaag ttcagggcta gggtgatggt gtccagaacc acacctaaag 11760
aggtgacagt cacaacagac tatgatggta gtcaggacat cttggaatac gagtgggttg 11820
actttgagtt accagaaggc aacttctctg ccaccatgac catagacctg atgaataatg 11880
caattgttga taattaccta aaagtgggta gacagaatgg ggtactggag agtgacatag 11940
gtgttaagtt tgacactagg aactttaggc ttggttggga cccagtgaca gagttggtca 12000
tgcctggggt ctacaccaat gaagctttcc atcctgacat agtcctacta cctggctgcg 12060
gagtggactt cactgagagc cgcctcagta atctgctagg cattagaaag aaacagccat 12120
tccaggaagg gttccagatc atgtatgagg atctggaggg tggtaacatc cccgccctgc 12180
ttgatgtaaa tgcatatgag aagagcaagg aagataatac aaccaccaca aatgaagctg 12240
tggccgcggc ttcatctact gaagccaaag ctgtggtaga tgcttccact tcaacagaaa 12300
acaccactga tgaaaaagtc accaggggag atacatttgc cacccctgaa caagagaagg 12360
cagctgaggc agagtctgat attatgcttc tgtccaccga tgaaaacgaa actaaaaaac 12420
aactggttat tcgagcggtg accaaggata gtaaggacag gagttataat gtattgtcag 12480
atggaaagaa cacagcttac cgtagttggt acctggcata caattatggc gaccgtgaga 12540
aaggggtgcg ttcttggaca ctgcttacca cctcggatgt cacctgcggc gtggagcaag 12600
tctattggtc gctaccagat atgatgcaag atccagtcac ctttcgctcc acacgccaag 12660
ttagcaacta cccagtggtg ggcgcagagc tgctcccagt gcattccaga agcttctaca 12720
acgagcaagc cgtctactcg caacagctcc gccagtacac ctcgctcacg cacgtcttca 12780
accgcttccc cgagaatcag atcctcgtcc gcccgcccgc gccaaccatt accaccgtca 12840
gtgaaaacgt tcctgctctc acagatcacg ggaccctgcc gctgcgcagc agtatccggg 12900
gagtccagcg cgtgaccgtt actgacgcca gacgccgcac ctgtccatac gtatacaagg 12960
ccctgggcat agtcgcgccg cgcgtccttt caagccgcac tttctaaaaa aatgtccatt 13020
ctcatctcgc ccagtaataa caccggttgg ggcctgcgca cacctagcaa gatgtatgga 13080
ggcgctcgca gacgctccac tcagcaccct gtgcgcgtgc gcgggcattt ccgcgctccc 13140
tggggcgccc tcaagggacg ctctcgtact aggaccaccg ttgacgatgt gatcgaccag 13200
gtggtcgccg atgcacgtaa ctataccccc gcagccgcac ctgcatccac cgtggatgcg 13260
gtcattgaca gcgtggtagc cgatgcgcgc gcctatgctc gcgccaagag caggaggcgg 13320
cgtattgcca ggcgtcaccg agctactcca gccatgcgag ctgcaagagc tttattgcgg 13380
agagccagac gtgtggggcg aagagccatg cgtagagcgg ccagacgcgc ggcttcaggt 13440
gccagcgcag gcagggtccg caggcgcgcg gctacggcgg cagcggcggc catcgctagc 13500
atgaccaaac cacgaagagg caatgtgtat tgggtgcgcg acgccgccac cggccagcgc 13560
gtgcccgtgc gcacacgccc ccctcgcact tagaagatac tgagcagtct ccgatgttgt 13620
gtcccagcgg cgagatgtcc aagcgcaaat tcaaggaaga gatgctccag gtcatcgcgc 13680
ctgagatcta cggtcctgcg gtgaaggatg aaaaaaagcc ccgcaagatc aagcgggtca 13740
aaaaggacaa aaaggaagaa gatggtgatg atgggctggt ggagtttgtg cgcgagtttg 13800
ccccaaggag gcgcgtgcag tggcgcgggc gcaaagtgtg gccggtgttg agaccgggga 13860
ccacagtggt ctttacgcca ggcgagcgct ccagcaccgt ttccaaacgc tcttatgatg 13920
aggtgtacgg ggacgatgat attctcgagc aggcggctga tcgccttggc gagtttgcat 13980
atggcaaacg cagccgctcg ggagccaagg aagaggcatt gaccatcccc ttggatcatg 14040
gaaatcccac cccaagcctc aaacccgtga ccctgcaaca agtgctgccc acgccgccac 14100
gcaagggcat caagcgcgag ggcgaggatc tgtatcccac catgcagctg atggtgccca 14160
agcgccagaa gctggaagac gtgctggaga aaatgaaagt ggatcctgaa atccagcctg 14220
aagtcaaagt gaggccaatc aagcaggtgg cgcccggttt gggggtacaa accgtggata 14280
tcaagatccc caccgagtcc atggaaattc aaaccgaacc catgaagccc acctccagca 14340
ccattgaggt gcagacggat ccttggatgc ccgcgcctgc tcctgttacc actactactc 14400
gaagacctag aagaaagtat ggttcagcca acctgataat gccaaactat gctctgcatc 14460
catcaatcat acccactcct ggctaccgcg gcactcgcta ctaccgcagt cacagcaccc 14520
gccgacgtaa agcacctgcc acccgccgcc gtcgccgccg ccgtgccact agcaaactta 14580
ccccctcggc tatggtgcgg agagtgtacc gtgatgggcg cgcagctcct ctgacactgc 14640
cgcgcgcgcg ctaccatcct agcattgcca tttaacaact ctgcctcctt gcagatatgg 14700
ccctcacttg ccgccttcgt attcctattg ctggctaccg cggaagaaag tcgcgccgta 14760
gaagagcagg gttgtctggg agcgggatgc gtcgccaccg gcggcggcgc gccatcagca 14820
aacggttggg gggtggattt cttcccgctt tgattcccat catcgccgcg gcgatcggcg 14880
cgataccagg catagcttcc gtggcggtgc aggcctcgca gcgccactga cattggaaaa 14940
agatatctta taaataaaaa tagaatggac tctgacgctc ctggtcctgt gatatgtttt 15000
tgtagacgag atggaagaca tcaatttttc atccctggct ccgcgacacg gcacgcggcc 15060
gtatatgggc acctggagcg acatcggcaa cagccaactg aacgggggag ccttcaattg 15120
gagcagtcta tggagcgggc ttaaaaattt tgggtccact ataaagactt atgggaacaa 15180
agcttggaac agcagcacag ggcatgcgct gagacaaaag cttaaagatc agaatttcca 15240
acagaaggtg gtcgatggta tcgcctctgg aatcaatggg gtggtagatc tggccaacca 15300
ggccgtgcag aaacagatta acagtcgcct ggacccggct cccccagctc ctattcatga 15360
gttaatgcaa gtggaggaag agctcccttc attggaaaag cggggcgata agcgacctcg 15420
tccagatatg gaggaaacgc tgctgaccaa ggtggatgag ccgccctcct atgaagaggc 15480
tgtaaaactg ggaatgccca ctacaaagcc cattatgcct ctggccactg gagtgatgaa 15540
gccatctcag tctaaacctg cagttgctgc tacattggac ttgcccgctc ccgtggccac 15600
ccccaaacct gtcgccgccc cgaagcccac cgccgtgcaa cccgtggccg tggccagacc 15660
gcgtcccggt ggtcggccga atgcaaactg gcagagcact ctgaacagca tcgtgggttt 15720
gggagtgcac agtgtgaagc gccgtcgctg ctattgatta aatatggagt agcgcttaac 15780
ttgcttgtct gtgtgtgtat atgtcgatgc cgcccgccgt gctacagcaa agagagaagg 15840
agaagaggcg ccgctgagtt cctttcaaga tggccacccc atcgatgctg ccccagtggg 15900
cgtacatgca catcgccgga caggacgctt cggagtacct gagtccgggt ctggtgcagt 15960
tcgcccgcgc cacagatacc tacttcaatc tggggaacaa gtttaggaac cctaccgtgg 16020
ctcccaccca cgatgtgacc accgaccgta gccagcgcct gacgctgcgc tttgtgcccg 16080
ttgaccggga ggacaatacc tactcctaca aagtcagata caccctggct gtgggagaca 16140
acagggtgtt ggatatggcc agcacctact ttgacatcag gggcgtgttg gacagaggac 16200
ctagcttcaa accatactct ggcactgcct acaactccct ggctccaaaa ggagctccaa 16260
actccagtca gtggcaacaa aaggaaaaca atggtcaagg tgatgcaaag actcacacct 16320
atggtgtagc tgccactgga ggtattgaca ttgacaaaaa tggtcttcaa attggaatcg 16380
atgaaactaa agaagataat aacgaaattt atgcagacaa aacattccaa cctgaacctc 16440
aaattggaga agaaaactgg caagatagcg aaaactatta tggaggcagg gctcttaaac 16500
cggaaaccaa gatgaagcct tgctatggtt ccttcgctag accaactaat gcaaagggag 16560
gtcaagccaa aattaaacca gctcaagagg gtcaacagtc tatagattat gacatagacc 16620
tggctttctt tgatattcca agcactggcg gaggcaatgg cacaaatgta aatgacaagc 16680
cagatatggt tatgtatact gaaaatgtaa atctggaaac tccagacact catcttgttt 16740
acaagccagg aacttcagat gacagttccg aggccaattt aactcagcaa gccatggcta 16800
acagacccaa ctatattggg tttagagata actttattgg cgtcatgtac tacaacagca 16860
ctggcaacat gggagtgctt gctggtcaag catcccagct aaatgctgtg gtggacctgc 16920
aagacagaaa caccgagctg tcttatcagc tattacttga ctctctgggc gacagaacca 16980
ggtattttag tatgtggaat caggcggtgg acagctatga tcctgatgtg cgcattattg 17040
aaaaccatgg tgtggaagat gaattgccaa actattgctt cccattggac ggagctggca 17100
ctaatgctgt ttaccaagga gttaagacaa aagaggataa taatggcgaa tgggaaacag 17160
acacaaatgt tgcatcgcag aatcagatat gcaagggcaa catatatgct atggagatca 17220
acctgcaagc caacctgtgg aaaagtttcc tttactccaa cgtggctctg tacctaccag 17280
actcctacaa gtacactcca tccaacgtga cactccctac caacactaac acctatgact 17340
acatgaatgg cagggtggtg tctccatccc tggtggatgc ctacattaac attggcgcca 17400
ggtggtctct ggatgccatg gacaatgtca accctttcaa ccaccaccgc aatgccggcc 17460
tgcgctaccg gtccatgctt ctgggcaacg gccgatacgt gcccttccac atccaagtgc 17520
cccagaaatt cttcgctatc aagaacctgc tgcttctccc aggctcatac acctacgagt 17580
ggaacttccg caaggatgtc aacatgatcc tgcagagttc ccttggcaat gacctcagaa 17640
ccgatggggc caccatccag tacaccagca tcaatctcta tgccaccttc ttccccatgg 17700
ctcacaacac tgcctccacc ctggaagcca tgctgcgcaa tgacaccaat gaccagtcct 17760
tcaatgacta cctctcagct gccaacatgc tttaccccat ccctgccaat gccaccaacg 17820
tgcccatctc catcccatct cgtaactggg ctgccttcag gggctggtct ttcacccgcc 17880
tcaagaccaa ggagacccca tctctgggat cagggttcga tccctacttc gtctactcag 17940
gctccattcc atacctggat ggaactttct accttaacca cactttcaag aaagtctcca 18000
tcatgtttga ctcttctgtc agctggccag gcaatgacag gctgctgact cccaatgagt 18060
tcgaaatcaa gcgcactgtt gatggggaag ggtacaatgt ggcacaatgc aacatgacca 18120
aagactggtt cctggttcag atgctctccc actacaacat tggctaccag ggcttctaca 18180
tcccagaagg atacaaggac cgcatgtact ccttcttcag aaacttccag cccatgagcc 18240
gccaggtggt cgatcaggtc aactacaaag actacatggc agtcaccctt gcctatcagc 18300
acaacaactc tggctttgtg ggctacctcg cgcccaccat gcgacagggc caaccctacc 18360
ctgctaacta cccatacccg ctcattggca agactgcagt caacagtgtc acccagaaaa 18420
agttcctctg cgacagggtc atgtggcgca tccccttctc cagcaacttc atgtccatgg 18480
gggcccttac cgacctgggg caaaacatgc tttatgccaa ctccgcccac gcgctagaca 18540
tgaatttcga agtagacccc atggatgagt ccacccttct ctatgttgtc ttcgaagtct 18600
tcgacgtggt cagagtgcac cagccccacc gcggcgtcat cgaagctgtc tacctgcgca 18660
cccccttctc agctggtaac gccaccacat aagcgccttg cttcttgcaa gtggctgcag 18720
cagcatggcc tgtggatcct ccactggatc caatgagcaa gagctcaggg ccatcgccat 18780
agacctgggc tgtggaccct atttcctggg aacctttgac aagcggtttc caggcttcat 18840
ggctcctgac aagctcgcct gtgccattgt caacacggca gggcgcgaga ctggtggtga 18900
gcactggctg gcttttggat ggaacccccg ctccaatacc tgctatctct ttgacccgtt 18960
tgggttttca gacgagcgcc tcaagcagat ctatcaattc gagtacgagg ggctcctgcg 19020
ccgcagtgcc ctggctacta aggaccgatg catcactctg gaaaagtcta cccagaccgt 19080
gcagggtccg cgctcggctg cctgcgggct cttctgctgc atgttcctcc atgcttttgt 19140
gcactggccc gaccgcccca tggacaacaa ccccaccatg aatttgctga cgggggtacc 19200
caacaacatg ctccaatcgc cccaagtaga gcccaccctg cgccacaacc aggaggcact 19260
ctatcgcttc ctgaactccc actcatctta ctttcgttct aaccgcgcgc gcattgagaa 19320
ggccactgcc ttcgatcgaa tgaataataa catgtaaacc aaattgtgtg tggctcaaat 19380
aaacagcact ttattgttta catgcactga ggctctggga tgatcatttt ttaaaaatcg 19440
aaggggttct ggcgggaatc agcatggcca gatggcaggg acacgttgcg gaactggaac 19500
ttgttctgcc acttgaactc gggaatcacc agcctgggaa ctggaatctc tggaaaggta 19560
tcttgccata gctttctggt cagttgcaga gcgccaagca ggtcaggagc agatatcttg 19620
aaatcacagt tggggccaga attctgggcg cgggagttgc ggtacactgg gttgcagcac 19680
tggaacacca taagggcagg gtgtctcacg ctcgccagca cggtctcgtc actgatgcaa 19740
gacacatcca ggtcttcagc attggccatt ccaaaggggg tcatcttgca ggtctgtctg 19800
cccatcacgg gagcgcagcc aggtttgtgg ttgcaatcac aatgaagggg gatcagcatc 19860
atcttggcct ggtcgggggt aatccctggg taaacagcct tcatgaaggc ttcatactgc 19920
ttgaaagctt cctgggcttt ggttccctcg gtgtagaaca ctccacaaga cttgctggaa 19980
aactgattag tagcgcagtt ggcatcattc acacagcagc gggcgtcgtt attagccagc 20040
tggaccacat tcctgcccca gcggttctgg gtgatcttgg ctcgatctgg gttctccttc 20100
aacgcgcgct ggccgttctc gctcgccaca tccatctcaa tgacatgttc cttctggatc 20160
atgatgttgc catgcaggca tctaatcttg ccttcataat cagtgcagcc atgaggccac 20220
agcgcgcacc cggtgcactc ccaattgtta tgggggatct gggaatggct atgaaccagc 20280
ccttgcagga atcttcccat catcacagcc agggtcttta tgctggtaaa ggtcagcggg 20340
ataccgcggt gctcctcgtt cacatactgc tggcagatgc gtctgtagtg ctcggcctgc 20400
tcgggcatca gcttgaaaga ggttttcaac tcattatcca gcctgtatct ctccatcatg 20460
atggacatta cttccatgcc cttctcccag gcagaaacaa tagggagact caggggattc 20520
ttgacagtag agacaacctt acttaagggg tcatcactgc caatcttttc gatgcttctc 20580
ttgccatcct tctcggtgat gcgcaccggc gggtagctga atcccacagc caccaactga 20640
gcctcttccc tttcgtcttc gctgtcttga ctgatgtctt gcagaggaac atgtttggtt 20700
ttcctgggtt tcttcttggg cggcagctct ggaggactct ggctccgttc cggagacccc 20760
atggatgagc gagagttgtc gctcaccact tggatctggc tgcctgtaga agaactggac 20820
cccacgcggc ggtaggtgtt cctcttggta ggcagaggtg gaggcgacgg gctccggtcc 20880
ggtctgggtg gcggatggct ggcggagccc cttccgcgtt cgggggtgcg ctccagatgg 20940
cggtcgtctg actgacctcc gcggctggcc attgtgttct cctaggtaga gaaacaagac 21000
atggagactc agccatcgct gccatcgcca tccaccacca caagcaccgc cgaggaggag 21060
gagtgtttaa ccaccccacc atgcagcccc gctaccacca ccagcaccct tgaaagcgag 21120
gtcgacacgg tcgtggagga tttacaggct atggaagata ttgaggcagc tgtcgagcaa 21180
gaccccggct atgtgacacc ggcggagcat gatgaggatc tagcgcgctt tctcgacggt 21240
gtggagaaag cgaaacaaga tgaggacgag gaagaggcag aagcacaacc atcggtggcc 21300
gactacctca ccggcctagg gctagaagac gtgctgctta agcatcttgc aaggcagaca 21360
gtcatagtca aagacgccct gctagagcgc tccgaggtgc cactcagtgt ggaagacctc 21420
agtcgcgcct atgagctaaa cctcttctcg cctcgcaagc cccccaagcg tcagcccaac 21480
gggacctgtg agcccaatcc gcgcctcaac ttctatccag ccttcactgt gcccgaagta 21540
ctagctacct accacatctt tttcaagaac caaaagatcc ccatctcctg ccgcgccaat 21600
cgcacccgcg cagatgccct actcaacttg gggcccggcg ctcgcatacc tgatatcgct 21660
tccttggaag aggttcctaa gatctttgag ggtctgggca atgaggaaac tcgggcagca 21720
aacgctctgc aaagagaaac agatgatggt gaacaccaca gcgctctggt ggagctccag 21780
ggcgacaacg ctcgtcttgc agtcctcaaa cgcagcatcg aggtcaccca tttcgcctac 21840
cccgcactta atctcccacc caaagtcatg agctcggtca tggacacgtt gctcatgaag 21900
cgcgcgagcc ccatctccga ggatcagaac atgcaggacc ccgatgcctc agatgaaggc 21960
aagcctgtag tcagcgacga gcaactggct cgctggctag gctctgactc cccccagtct 22020
ttggaggagc ggcgcaagct tatgatggca gtggtcctga tcacagcgga gctggagtgt 22080
ctccgccgct tcttcactga cccagagacc ctgcgcaagc ttgaggagaa cctgcattac 22140
acattcagtc atgggttcgt gcgccaggcg tgcaagatct ccaacgttca actcaccaac 22200
ctggtctcct acctgggcat cttgcatgaa aaccggctgg ggcagaacgt gctccacacc 22260
accctgaagg gggaggcccg ccgcgactat atccgcgact gtatctacct ctacctatgc 22320
tacacctggc aaagcgggat gggtgtgtgg caacagtgct tggaagagca aaatctaaaa 22380
gagctggaaa agctgcttca gaaatctctt aaatctctgt ggaccgggtt cgatgagcgg 22440
accaccgctt cggacatggc cgatattatc ttccccgagc ggctcagaca cactctgcgc 22500
gacgggctgc ctgactttgc cagccagagc atgctacaaa actttaggtc attcatcttg 22560
gaacgctccg ggatcctgcc cgccacttgc tgcgcactgc cctccgattt tgtgcccatc 22620
acctaccggg agtgcccccc gccgctatgg agccactgct acctgttccg cctggccaac 22680
tacttggcct accactctga tgtgatagaa gatgttagtg gcgaagggct cctggagtgc 22740
cactgccgct gcaacctctg caccccccac cgctccctcg cctgcaatcc ccagctgctg 22800
agcgaaaccc agatcatcgg caccttcgag ttgcaaggtc ccagcggcga aggcgagggg 22860
tcctctccgg ggcaaagtct gaaactgact ccggggctat ggacctccgc ttaccttcgc 22920
aagttcgccc ccaaagacta ccacccctat gagatcaggt tttatgaaga ccaatcacag 22980
ccccccaagg ccgaactgac ggcctgcgtc atcacccagg gggcaatctt ggcccaattg 23040
caagccatcc aaaaatcccg ccaagaattt ttgctgaaaa agggacacgg gatctatcta 23100
gacccccaga ccggtgagga gctgaataca cgcttccctc aggatgcccc gaggaggcaa 23160
gagaatgaaa gttcagatgc cgcccgagga ggagctggaa gactgggaca gtcaggcaga 23220
ggaggaagac tgggacagcc aggcagaaga ggaggacagc ctggaggagg acagtctgga 23280
ggaaggcgag gagcccaagg aagaggcagc cgccgccaga ccatcgtcct cggcggtgga 23340
gacaagcaag gtcccagaca gcacggctac cacctccgct ccagctcaag gggccgctcg 23400
gcgacccaac agtagatggg acgagacggg tcgcttccag aaccccacca ccgtcaagac 23460
cggtaagcag gagcggcagg gatacaagtc ctggcggggg cataaaagtg ccatcatcgc 23520
ttgcttgcag gagtgtgggg gcaatatatc ctttgccaga cgctacctgc tattccatca 23580
cggggtgaat ttcccccgca acatcttgca ttactaccgt cacctccaca gcccctacta 23640
ccagcagcaa gagacagcag aggaaaccag cggcaactcc gagagttaga aaaccagcag 23700
ctaaaaaatc cacagcggcg gcagcaggtg caggcggact gaggatcacc gcgaacgagc 23760
cagctcagac cagggagttg aggaatcgga tctttcccac cctctatgcc atattccaac 23820
aaagtcgggg tcaggaacaa gaactgaaag taaaaaacag atctcttcgc tcgctcaccc 23880
gcagttgttt gtatcacaag agcgaagacc aacttcagcg cactctcgag gacgccgagg 23940
ctctcttcaa caagtactgc gcgctgactc ttaaagagta gactgcgcgc gcttggcgag 24000
aaaaggcggg aattacgtca cctcttggcc acacctgtgc ttcattatga gtaaagaaat 24060
tcccacgcct tacatgtgga gctatcagcc ccagatggga ttggccgctg gcgccgccca 24120
ggactactcc acccgcatga attggctcag cgccggtccc gcgatgatct cacgggttaa 24180
tggtgtgaga gagcaccgaa accagatact cctagaacag tccgccctca ccgccactcc 24240
ccgcaatcac ctcaaccccc gtaattggcc cgccgccctg gtgtaccagg aaactcctgc 24300
tcccactaca gtactacttc ctcgtgacgc ccaggccgaa gttcagatga ctaactcagg 24360
tgtacagctg gcgggtggtg ccaccctgtg tcgtcaccgg ccaagaccgg gtataaaggg 24420
cctggtgatc agaggccgag gtattcagct caacgacgag tcggtgaact cttcgcttgg 24480
tctgcgacca gacggcatct tccaaatagc tggttgtggg agatcttcct tcactcctcg 24540
tcaggctgtc ctgactttgg agagttcgtc ctcgcagccc cgctcgggcg gcatcgggac 24600
tctccagttc gtggaggagt ttactccctc ggtctacttc aaccccttct ccggttctcc 24660
tgggctttac ccggacgagt tcatcccgaa ctacgacgcc atcagtgaag cggtcgacgg 24720
ctacgattaa tgtctaatgg tggcgcggct gagctagctc gactgcgaca cctagaccac 24780
tgccggcgct ttcgctgctt tgctcgggat ctctgcgagt tcatctactt cgagtaccct 24840
gacgaacatc ctcagggacc tgcccacgga gttcggatta ccattgaagg ggctatcgat 24900
tctcacctgc ttcggatctt caccgctcgg ccagtgctag ttgagcgcaa ccagggcgac 24960
accaccatct ccctctgctg catttgtgac aaccccggat tgcatgaaag cttttgttgt 25020
cttctttgta ctgagtataa taaaagctga aattagagac tactccggac tctcttgtcg 25080
tctgaacaac accaaccaga cccttcactt cagcgggaac cagactactc ttcactgtaa 25140
ggcttataac tataagtatc ttacttggat atacaaagga acaccgtttg ctgtggtaaa 25200
caggtgctcc aacgacggtg ttctcctcac cttcctaggc aacttctcca actttacctt 25260
ttctgttcgc agaaacaagc ttaccctcct tcagccctac tttcctggga tctatacctg 25320
cctcagtgga ccttgcaacc acacttttca cctgattgaa aactctaccc ttaccttccc 25380
agcgccaatc cctactaaca gctcggagtc caactcttcc attaccgctg atactaacac 25440
tcctaaaacc ggaggtgagc tccgcagcct tcccccggct gcagataacc cttgggtggt 25500
agcgggattt gtagcgctag gaatagttgc gggtgggctc gcgttcgtcc tctgctacct 25560
ataccttacc tgctgctcat atttagtagt actgtgctgt tggtttagaa aatgggggcg 25620
ctactaatca cacttgcttt actttcgctt ttgggtctga gctcggctaa tagcgagaaa 25680
ccaagctgtc tagaaacaaa ctctccagac tgtgtggttc ctcatgggct ctcagaccca 25740
gctgatgatc catgcttaac ttttgaccca gaaaaaaact gctcggtgac tatgcagccc 25800
tatgcttaca tgtgcacatc tgttataaag tgcggatggg gctgtaaacc gattgaaatt 25860
acccacaaag gcaaaacctg gaataatagt ttgtttaaca catggcagcc tggagacgag 25920
cagtggtata cggccggcca ctggtggaga tgactgaccc catggaaaac tcctctgcca 25980
acgacctgga catggacggc cgttcatctg agcagcgact ggtccagatg cgcattcgcc 26040
agaagcagga acgcgccgcc agagagctca aggatgccat tgaaattcac ctgtgcaaga 26100
agggcatctt ttgcttggtt aagcaagcaa agatttctta tgaaatcact gacaacgacc 26160
accgcctgta ttatgagctc ggtccacagc ggcagaaatt cacctgcatg gttggagtca 26220
accccatagt catcactcag caggctgcag aaattaaagg gtgcatccac tgttcctgtg 26280
attcccaaga atgcgtccac accatagtca agaccctctg cggccttcga gatcttcttc 26340
caatgaacta accccttccc ccaacccaat aaaacattgg ttttaatcat aataaaaaat 26400
cacttacttt aaatctgaaa cagtgtctcc gtccaagttt tcttgtagca ccacttcact 26460
cccctcttcc cagctctggt actgcaagcc ccggtgggct gcaaactttc tccacacctt 26520
aaaagggatg tcaaattcct cttgtccaac aatcttcatt gtctcttcct agatgtccac 26580
aaagcgcgcg cgggtggaag atgactttga ccctgtctac ccatacgatg ctgagctggc 26640
accgtctgta cccttcatcg cccctccctt cgtttcgtca gacggatttc aagaaaaacc 26700
cctgggagtt ctgtccctaa gactagccaa cccagtcact actaaaaatg gggaactcac 26760
acttaaactg ggagatgggg tgggcataga ctcagatgga aacctcacag cacagacagt 26820
tactaaagca acatcccccc ttactgtttc caataacgca attgcactta acatggacaa 26880
acctttttac agtagcaatg gaaaactatc cttacaagtt acatcaccat taaagatagt 26940
cgactcttta aatacattgg ctattggcta tgggcaaggc ttaggactaa acaatagtgc 27000
tcttgctgtg caattagcat ctccccttac atttgacagc aacagcaaaa ttaaaataaa 27060
tttgggaagc gggccattaa aaattaatgc gaataaactg tcaattaact gcctaagggg 27120
tgtatatgta acaactgacg gaacttccat tgaaacaaat ataagctggg caaaaggaat 27180
gaggtttgaa ggtaatgcca tggctgtaaa cgttgacagc accaaaggtc tacaatttgg 27240
cactaccagc acagaatcag gagtcactaa cgctttccct atccagttaa agattggatc 27300
tggtcttagt tttgacagca caggagcact tgtagcttgg gataaggata atgacaagct 27360
tacactgtgg acaaccgctg acccatcacc taattgtacc atatatacag acaaggatgc 27420
taaacttaca ctttgtctta caaaatgtgg cagtcaaata ctaggcagtg tttcagtact 27480
ggctgttaaa gctggaaccc tacagccaat cagtgaaaaa ataggtactg ctttggtttc 27540
actaaaattt aataacaacg gtgtattgtt aagcaactcc acattaagta atgaatactg 27600
gaactacagg aagggagatg tcacaccagc cgaagcctat actaatgctg tgggttttat 27660
gccaaacatc aaggcatatc ctaaaaacac aaactctgcc tcaaaaagcc acattgtagg 27720
acaagtgtac cttaatggag atgaaactaa accaatgcat ttaatcatta catttaatga 27780
aaccagtgat gaaacatgca catattccat aacgttccaa tggaaatgga acattggaac 27840
atacaccagc gacacccttg caacaagctc ctttaccttt tcttacattg cccaagaata 27900
aaaactgcag acaacaataa agtttaaatg ttttatttaa acagtttcac agaaccctag 27960
tattcaacct gccacctccc tcccaacaca cagagtacac agtcctttct ccccggctgg 28020
ccttaaaaag catcatatca tgggtaacag acatattctt aggtgttata ttccacacgg 28080
tttcctgtcg agccaaacgc tcatcagtga tattaataaa ctccccgggc agctcactta 28140
agttcatgtc gctgtccagc tgctgagcca caggctgctg tccaacttgc ggttgcttaa 28200
cgggcggcga aggagaagtc cacgcctaca tgggggtaga gtcataatcg tgcatcagga 28260
tagggcggtg gtgctgcagc agcgcgcgaa taaactgctg ccgccgccgc tccgtcctgc 28320
aggaatacaa catggcagtg gtctcctcag cgatgattcg caccgcccgc agcataaggc 28380
gccttgtcct ccgggcacag cagcgcaccc tgatctcact taaatcagca cagtaactgc 28440
agcacagcac cacaatattg ttcaaaatcc cacagtgcaa ggcgctgtat ccaaagctca 28500
tggcggggac cacagaaccc acgtggccat cataccacaa gcgcaggtag attaagtggc 28560
gacccctcat aaacacgctg gacataaaca ttacctcttt tggcatgttg taattcacca 28620
cctcccggta ccatataaac ctctgattaa acatggcgcc atccaccacc atcctaaacc 28680
agctggccaa aacctgcccg ccggctatac actgcaggga accgggactg gaacaatgac 28740
agtggagagc ccaggactcg taaccatgga tcatcatgct cgtcatgata tcaatgttgg 28800
cacaacacag gcacacgtgc atacacttcc tcaggattac aagctcctcc cgcgttagaa 28860
ccatatccca gggaacaacc cattcctgaa tcagcgtaaa tcccacactg cagggaagac 28920
ctcgcacgta actcacgttg tgcattgtca aagtgttaca ttcgggcagc agcggatgat 28980
cctccagtat ggtagcgcgg gtttctgtct caaaaggagg tagacgatcc ctactgtacg 29040
gagtgcgccg agacaaccga gatcgtgttg gtcgtagtgt catgccaaat ggaacgccgg 29100
acgtagtcat tctcgtactt tgagtggcaa aaccttgctc tcgaacagca cacgtctcgt 29160
cgcctcctgt cccttctctt cgccttttca gtgtgatagt tgtaatacag ccattcacga 29220
agctcagtca gaagatcttc agcgtctgtt gtcaaaaaca atccatccaa tctgattgct 29280
ttcaaaacat cacaaacagt cgaataagcc aaacccatcc aggcaatgca attattttgg 29340
ttatccacaa tgggaggggg cggaagacat ggaagaggca taattaattt tttaatccaa 29400
tcgatcacgc agcacttcaa aatgaagatc gcgaaggtga cacctttcac ccccactgtg 29460
ttgatgaaaa ataacagcca agtcaaaatt gatgcggttt tcaaggtgct cgactgtagc 29520
atcaagcaga gcttccacac gcacgtccac aaataacaga atagcaaaag cgggaggagg 29580
aagtaaatcc tcaatcatca tagtacagtc catcaccatc cctaaataat tttcatcctt 29640
ccagccttgg actatatttt taaactgctc ttgtaaatcc aaaccacaca tgtggaaaag 29700
ttcccaaaga gctccctcaa ctaccattct taagcacacc ttcatagtga caaaatatct 29760
tgttcctctg tcacctgcag caaattacaa agtccaatat taggatctat gcccagagat 29820
ctaagctcat ccctcaattc caactgtaaa aaggcttcca gatctgccct aacttgttca 29880
gccagtgggc tccctggaat aagcgtggga gaagccaaac tgcaaaacag acgcatgccg 29940
ccataattac caccagaaaa cactacgtta cagtatgcat gctgattcat tccagtaatt 30000
tcatccagtg tattggatac aaaaaaaggc aagcactctc tcactaattg tattatggag 30060
acattatcac acaggtaaca atttaaaggt tgtggaacaa taatgcagta agtaaccacg 30120
gtgcgctcca acatggttag taatttttag ttctgaaaaa caaaacatac aaaaaattat 30180
atcatactca tttggcgaac tggtggaaaa atgaccctat ctagcacaag gcaagccact 30240
ggatcaccaa tgcgcccctc ataaaacctg tcatcatgat taaaaagcaa caccgaaagc 30300
tcttccctat gtcctgcatg aatgattcta gctgaggaat ataagccagc gcaattagta 30360
tctgttaaag aaaaaaaacg gccaacatag cctctaggaa ttagcacact taatcttaaa 30420
gacattactg ccatccccct tggatttaag gtaaaattta caggagcata gaaaatatac 30480
tgatttccct cctgcacagg cagcatagca ccaggtccct ctaaaaacac acacaaagct 30540
tctgcagcca tagcttaccg cgcaaaccag gcacagcagt gagctaaaag gacaaagctc 30600
taactcacta gccaacctgg cgcacaatat atagttagtc cttacactga cgtaaccgac 30660
caaagtctaa aaaccccgcc aaaaatacac acacgcccaa aaaacgcccc gtgagtcaaa 30720
aaacagtttc acttcctcgt tacacccaaa acgtcgtcac ttccggattc ccacggttcg 30780
tcacttccgg agctccttgc ttaattaacc ccgcccaaaa cgtcatcgtc cgcgtcacgc 30840
cgccccgccc cgcgaccgtt gaccccgggc caatcaccgc acatcccgca aaattcaaac 30900
tcgtctaatt tgcatattgg cacactgccc atataaggta tattattgat gatg 30954
<210> 14
<211> 30615
<212> DNA
<213> 人工序列
<220>
<223> BLY6.dE1.dE3.5IXP腺病毒载体基因组
<400> 14
catcatcaat aatatacctt atatgggcag tgtgccaata tgcaaattag acgagtttga 60
attttgcggg atgtgcggtg attggcccgg ggtcaacggt cgcggggcgg ggcggcgtga 120
cgcggacgat gacgttttgg ggaggaggag ctatgttgca agtaatcgtg ggaaatgcga 180
cgtaaaacga ggtggagttt aaacacggaa gtagacaatt ttcccgcgct gtttgacagg 240
aaatgatgtg tttttgggcg gatgcaagtg aaaattctcc attttcgcgc gaaaactaaa 300
tgaggaagtg aaattctgag taattctgag gttatcacag ggcggagtat ttaccgaggg 360
ccgagtagac tttgacccat tacgtggagg tttcgattac tctatttttc acctaaattt 420
ccgcgtactg tgtcaaagtc cgtgttttta cgcgatcgcg gtactgaaat gtgtgggcgt 480
ggcttaaggg tgggaaagaa tatataaggt gggggtctta tgtagttttg tatctgtttt 540
gcagcagccg ccgccgccat gagtgggagc gcttcctttg aagggggcgt ctttagccct 600
tatctgacgg ggcgtctgcc tcattgggct ggagtgcgtc agaatgtgat ggggtctaca 660
gtggatggaa gacctgttca gcctgctaat tcttctactc tgacttatgc tactatgact 720
tcctcgcctt tggatgcagc tgcagctgct gccgcttctg ctgccgccaa cactgttcgg 780
gggatggcct tggagatggg gtattatgga actgtagtgg ccaacaccac taccccaaat 840
aaccccacag ccttgaatga ggacaagctg ctagttctca tgtcccagct ggagtctttg 900
acccaacgcc tgggcgatct agctcagcag gtgtcccagc tgaaggagca gactcaagct 960
gcaattacca ctgcgagggg aaattaaaaa aattcaaaga atcaataaat aaaccgagac 1020
tttgttgatt ttaaagtgtg tcattcttta tttaattttt cgcgcgcgat atgccctgga 1080
ccaccggtct ctatcattga ggacacggtg gatcttttct agaacccgat agaggtggga 1140
ttggatgttg aggtacatgg gcataagacc atctttgggg tgtagatagc tccactgcag 1200
agcctcatgc tccggggtgg tgttgtatat aacccagtca tagcatgggc gttgggcatg 1260
atgttgcaca atatctttaa ggaggagact aatggccact gggagaccct tggtgtaagt 1320
gtttacaaat ctattaagct gggacgggtg catccgaggt gagataatgt gcattttgga 1380
ttggattttt agattggcaa tgtttccccc tagatctctc ctgggattca tgttatgcaa 1440
gaccactaga acagtgtatc cggtgcactt agggaatttg tcatgaagtt tggaggggaa 1500
agcatgaaaa aatttagaca cacccttgtg tcctcccaag ttctccatgc actcatccat 1560
aataatggca atgggcccat gggcggcggc acgggcgaac acgttcctgg gatctgacac 1620
atcatagttg tggtcttggg tcaggtcatc ataagccatt ttaataaact tggggcggag 1680
ggtgccagat tgggggatga atgttccctc gggccccgga acatagtttc cttcacatat 1740
ttgcatttcc caggctttta gttcagaggg ggggatcatg tccacctgtg gagcgatgaa 1800
gaagacggtc tcgggggcgg gggtgattaa gtgggaggac agcaagttcc taagcagctg 1860
tgacttgcca cacccagtgg gaccgtagat gacccctata acaggttgca gatggtagtt 1920
tagggaaaga cagctgccgt cctctcgcag gaggggggcg acctcgttca tcatttccct 1980
cacatgcatg ttttcccgca caagttccga taggaggcgc tctccaccca gggaaaggag 2040
ttcttgaaga gatgagaaat ttttcaaggg ttttaagcca tcagccatgg gcattttgga 2100
gagggtttgt tgcaagagtt caaggcggtc ccagagttcg gtgatgtgtt ctatggcatc 2160
tcgatccagc atacttcctc gtttctgggg ttgggacggc tgcgggagta tggaaccagg 2220
cgatgggcgt ccagcgctgc cagtgtccgg tccttccacg gtcgcagcgt ccgagtcagg 2280
gtcgtttccg tcacggtgaa ggggtgcgcg cctggctggg cgcttgcgag ggtgcgcttc 2340
aggctcatcc tgctcgtgga gaaccgctgc cgttctgcgc cctgtgcatc ggccaggtag 2400
caattaacca tgagttcgta gttgagcgcc tctgccgcgt ggcctttggc gcgcagctta 2460
cctttggaag tcttctgaca ggtgggacag tagagacact tgagagcata gagttttggg 2520
gctagaaaga ccgattctgg ggagtatgca tcggccccac aggaggcgca gacggtttcg 2580
cattccacca gccatgtaag atcgggctcg ttggggtcaa aaacaagttt tccgccatgt 2640
tttttgatgc gtttcttacc tttgctttcc atgagttcgt gcccccgttg ggtgacaaag 2700
aggctgtccg tgtccccgta gactgacttt atgggcctgt cctcgagcgg cgtgccgcgg 2760
tcctcttcgt agaggaactc ggaccactct gagacgaaag cacgtgtcca ggccagcaca 2820
aaggaggcta tatgggaggg gtagcgatcg ttgtcaacca aggggtctac tttttccaag 2880
gtgtgtaaac acatgtcccc ttcttccaca tccaggaagg tgattggctt gtaagtgtat 2940
gccacgtgac ctggggtccc agacgggggg gtataaaagg gggcgggtct ctgctcgtcc 3000
tcactgtctt ccggatcgct gtccaggagc gccagctgtt gaggtaggta ttccctctcg 3060
aaggcgggca taacctccgc actcaggttg tcagtttcta ggaacgagga ggatttgata 3120
ttgacagtgc ctgccgagat gcctttcatg agactgtcgt ccatttggtc agaaaagaca 3180
atctttttgt tatcaagttt ggtggcgaag gatccataca gggcattgga aagcagtttg 3240
gcaatggagc gcatggtttg gtttttttct ttgtctgcgc gctctttggc ggctatgttg 3300
agttggacat attcgcgggc cagacatttc cattgtggaa atatggtagt taattcatct 3360
gggacgattc tgactttcca gcctctgtta tgcagggtaa tcagatccac actggttgcc 3420
acttctcctc taagtggttc attagtccag catagtcgcc ccccttttcg agaacagaaa 3480
gggggtaggg gatctagcat gagttcgtct ggggggtctg catctatggt gaaaatccca 3540
ggaaggagat cttcgtcaaa atagctgatg gtggcggggt catccagaga catttgccat 3600
tctcgagcag ccagagcgcg ctcgtagggg ttaaggggag tcccccatgg catgggatgg 3660
gtgagtgcag aagcatacat gccacagatg tcatagacat agagcggctc ttccagaatc 3720
cctatgtaag tgggataaca tcgcccccct ctgatgctgg ctcgcacata atcatagagt 3780
tcatgtgagg gcgctagaag acccgagccc aggttggtgc ggttgggttt ttctgctctg 3840
tagaggatct ggcgaaagat ggcatgggag tttgatgaga tggtgggtct ttggaagatg 3900
ttgaaatggg catgaggcag tcccacagag tcccttatga agtgagcata ggagtcttgc 3960
agtttggcca ccagctcggc ggtgaccagc acatccaaag cacagtagtc gagggtctct 4020
ttgatgatgt catagttagg ttcccctttc ttttcccaca gctcgcggtt gagaaggtat 4080
tcttcgcgat ccttccagta ctcttcgagg gggaacccgt ccttgtctga acggtaagaa 4140
cccagcatgt aaaattgatt gacagctttg taggcacaac accccttctc cacggggagt 4200
gagtatgctt gcgcggcttt gcgcagagag gtgtgagtaa gggcgaaagt gtccctgacc 4260
atgactttga ggaactgatg cttaaagtct atgtcatcgc aggccccctg ctcccacagt 4320
tggaagtcca ctcgcttttt gtaggcggga ttgggcaaag cgaaagtaac atcgttgaat 4380
aggatctttc cagccctggg catgaagttg cgagtaatgc gaaaaggctg aggcacttct 4440
gccctgttgt tgataacttg ggcagccaag acgatctcgt caaagccgtt gatgttgtga 4500
cccacaatgt aaagttctac gaagcgtggg cgtcccttga tgtggggcag ttttttaagc 4560
tcttcgtagg tcaagtcgtc agggtcagcg attccatatt gctccaaagc ccagtcaggc 4620
aggtgaggat tagcatgaag gaaagaggtc caaagatcca cggccagagc tgtttgtaag 4680
cggtctctgt actgacggaa atgtcggcct accgccattt tttcaggagt aacacagtaa 4740
aaggtgcgcg ggtccttttc ccagcgatcc cattgaagtt gcaaggctag gtcgtgggcg 4800
aggttgacga gctgttcgtc ccccgaaagt ttcatgacca gcatgaaagg gacaagctgc 4860
ttgccaaagg accccatcca ggtgtaggtt tccacatcgt aggtgaggaa gagcctttct 4920
gtgcgaggat gagaaccgat cgggaagaac tggatttcct gccaccagtt ggaggaatgg 4980
ctgttgatat gatggaagta gaactcccta cggcgcgccg agcattcgtg cttgtgcttg 5040
tacagacggc cacagtactc gcagcgctgc acgggatgca cctgatgaat gagctgtacc 5100
tggcttcctt tgacaagaaa tttcagtggg aagttgaggc gtggcgtctg catctcgtgt 5160
tgtattacgt cctggctatt ggtctggcca tcttctgtct cgatggtggt catgctgacg 5220
agcccgcgcg ggaggcaggt ccagacctcc gcgcggacgg gtctgagagc gaggacgaga 5280
gcgcgcaggc cggaactgtc cagggtcctg agacgctgcg gagtcaggtc agtagggaga 5340
gtacataggt ttacttgcat aagtttttcc agggcatgtg ggaggtcaag atgatatttg 5400
atttctactg gcgagttggt ggagacatcg atggcttgca gggtcccgtg cccctggggt 5460
gctaccaccg tccctttttt tttcttgatc gggggcggtg ttgcttcttg catggtaagg 5520
tcgtcttcta gaagcggcgg cgaggtcgcg cgccgggtgg cagtggcggt tctggacctg 5580
gaggtagagg cggtagaggt acgtcggcgc cgcgcgcggg taggttctgg tactgcgccc 5640
tgagaagact tgcgtgagcg acgacgcggc ggttgacgtc ctggatctga cgcctctggg 5700
tgaatgctac cggacccgtg agcttgaacc tgaaagagag ttcaacagaa tcaatttcgg 5760
tatcgttgac ggctgcctgc cgcaggattt cttgtacgtc gcccgagttg tcttggtagg 5820
cgatctcggc catgaactgc tcgatctctt cttcttggag atctccgcgg cccgctcgtt 5880
ctacggtggc agcaaggtcg ttggagatgc gccccatgag ctgtgagaat gcattcatgc 5940
ccgcctcgtt ccagacgcga ctgtagacca cggctccctc gggatctctg gcgcgcatga 6000
ccacttgggc gaggtttagt tccacgtgtc tggtgaagac cgcatagttg cagagacgct 6060
ggaagaggta gttgagcgtg gtggcgatgt gctcggtgac aaagaaatac atgatccagc 6120
gacgaagcgg catctcgctg atatcgccca gggcttccaa ccgttccatg gcttcgtaaa 6180
agtccacggc gaagttgaaa aactgggagt tgcgagcgga cacggtcaac tcctcctcca 6240
gaagacggat gagctcggcg atggtggcgc gcacttcgcg ctcaaaggct cccgggatct 6300
cttcctcctc ttcttcttcc aactcttcct ccactaacat ctcttctact tcctcctcag 6360
gcggcggggg tggaggaggg ggcgcgcggc gacgccggcg acgcacgggc agacgatcga 6420
tgaagcgttc gatcacttct ccgcggcggc gacgcatggt ctcggtgacg gcgcgcccgt 6480
cctccctggg tcgcagagtg aagacgccgc cgcgcagctc cctgaaatgg tgactgggag 6540
ggtccccgtt tggtagggac agggcactga tgatgcatct tattaattgc cctgtaggga 6600
ctccgcgcaa ggacctgagc gtctcgagat ccacgggatc tgaaaatctc tgaacgaagg 6660
cttcgagcca gtcgcagtcg caaggtaggc tgagcactgt ttcttcgggg cgggctgctg 6720
agctagaggg ttgtacgatg ctgctggtga tgaagttaaa ataggcagtt ctgagacggc 6780
ggatggtggc gaggagcacc aggtctttgg gtccggcttg ctggatgcgc aggcggtcgg 6840
ccattcccca tgcattatct tggcacctgg ccagatcttt atagtagtct tgcatgagtc 6900
gctccacggg cacttcttct tcgcccgctc tgccgtgcat gcgtgtgagt ccgtaccctc 6960
tctgtggttg gacgagcgcc aggtcggcaa cgaccctttc ggctagaatg gcttgctgca 7020
cctgggtgag ggtgttctgg aaatcatcaa agtccacaaa gcggtggtag gcccccgtgt 7080
tgatggtgta ggagcagttg gacatgaccg accagttgac tgtctggtgt cctggtcgta 7140
cgagttccgt gtacctgagc cgcgagtatg cgcgggagtc gaagatgtaa tcgttgcagg 7200
ttcgcaccag gtactggtag ccgatgagga agtgaggcgg cggctggcgg tagagaggcc 7260
atcgttcggt ggcgggcgcg ccgggcgcta ggtcttctag catgagacgg tggtatccgt 7320
agacgtacct ggacatccag gtaataccgg cggcggtggt ggaggcgcgc ggaaactctc 7380
gcacgcggtt ccagatgttg cgcagcggca tgaagtagtt catggtgggc acggtctggc 7440
ccgtgaggcg cgcgcagtca ttgatgctct agatacgggc aaaaacgaaa gcgttgagcg 7500
gttcccttcc gtggcctgga ggaacgcgaa cgggttaggt cgcagcgtac cctggttcga 7560
gactaaagaa agcgagcaac tcgaaccggc agagtcgcgg ctaacgggta ttggcaatcc 7620
cgtctcgacc caagccagca aatccaggat acggatgggg gccccttttg tttttcaggg 7680
catgagtcac cggttaaggt ttacaacggc tgtttcatgc ctttagaagt ggctcgcgcc 7740
cgtagtctgg agaatcaatc gccagggttg cgttgcggcg tgccccggtt cgagcctgca 7800
gcttgagtcg gccggtgacc gcggcaaacg agggcgtggc ggccccgtcg tttctaagac 7860
cttgctagcc gacctctcca gtttacggga acgagccccc ttttattttt tttgtttttg 7920
ccagatgcat cccgtactgc ggcagatgcg cccacagccc ccacagcagc agcagcaggc 7980
tggcctacct tctctacctc agccgctacc tgcaactacc gcggtggccg ctgtaagcgg 8040
ggccggacag caggcggctc ctcaatatga attggacttg gaagagggcg agggattggc 8100
aagattgggg gcgccctcgc ccgagcgcca cccgcgggtg cagatgaaaa aggacgttcg 8160
cgaatcttac gtgcccaagc agaatctgtt cagagacaga agcggcgagg agcccgagga 8220
gatgcgcgcg tcccgtttta acgcgggtcg cgagctgcga caaggactgg atcgaaaacg 8280
ggtgttgagg gatgatgatt ttgaggtgga tgaaatgaca gggatcagcc ccgctcgcgc 8340
tcacgtggct gcagctaatc tggtgacagc ttatgagcag accgtgaagg aggaaagcaa 8400
cttccagaaa tcattcaata accacgtgcg caccctgatc gcacgcgagg aggtgaccct 8460
gggcctgatg cacctgtggg atctgctgga agccatagtg cagaacccca ctagcaaacc 8520
cctgactgct caactgtttc tggtggtgca gcacagcagg gataatgagg cattcagaga 8580
ggcgctgctg aatatcactg aacctgaggg gagatggctg ctggatctgg tgaatatcct 8640
gcagagcatt gtagtgcagg aacgcagctt gcctttgtcc gagaaggtgg cggcgatcaa 8700
ttactctgtg ctgagtctgg gcaaatacta tgccaggaag atctacaaaa ccccttacgt 8760
gcccatagac aaggaagtga aaatagatgg gttttacatg cgcatgaccc tgaaagtgct 8820
aaccctgagc gatgacttgg gagtgtaccg caacgacagg atgcaccgcg cggtgagcgc 8880
cagcaggagg cgcgagctga gcgacaaaga attaatgcac agcttgcaac gagccctgac 8940
gggagccggg acggaggggg agaactactt tgacatgggt gcagacttgc attggctgcc 9000
tagtcgcagg gcattggaag cggcaggcga tgggccctat gtagaggaag tagtagacga 9060
ggacgatgag gagggcgagt acctggaaga ctgatggcgc gacccgtatt tttgctagat 9120
ggaacaggcg ccggaccctg cgatgcgggc ggcgctgcag agccagccgt ccggcattaa 9180
ttcctcggac gattggaccc aggccatgca acgcatcatg gcgctgacga cccgcaaccc 9240
cgaagccttt agacagcagc ctcaggccaa ccgcctttcg gccatcctgg aggccgtggt 9300
gccctctcgc tccaacccca cccacgagaa ggttctggcc atcgtgaatg ccctggtgga 9360
gaacaaggcc atccgctccg atgaagccgg gctggtatac aacgccttgc tcgagcgcgt 9420
ggctcgctac aacagcagca atgtccagac taacctggac aggatggtga ccgacgtgcg 9480
cgaggccgtg tcccagcgcg aacggttcca tcgcgagtct aacctgggtt ccatggtagc 9540
gctgaacgct ttcctcagtt cccagcctgc caatgtgccc cggggacagg aagactatac 9600
caactttatt agcgccctga gactcatggt agccgaggtt cctcagagcg aggtgtacca 9660
gtccggtcca gactactttt tccagacaag caggaacggt atgcagacag tgaacttaag 9720
ccaggctttc aagaacctgc aagggctgtg gggagtccaa gctccagtgg gcgacagggc 9780
gaccgtgtcg agcctgttga ctccaaattc ccgtttgctg ctgctgctgg tgtccccctt 9840
cactgacagc ggcagcataa acagaaactc ctacttgggc tacctgataa acttgtatcg 9900
cgaagctata ggtcaggccc acgtggacga acagacctat caggagatca ctaatgtgag 9960
tcgcgctctg ggccaggacg accctggaaa cctggaagct actctaaact ttctgctgac 10020
caaccgctcg caaaaaatcc ctcctcagta tacattaact gcggaggagg aacggatctt 10080
gagatacgtg cagcagagcg tgggtctgtt cctgatgcaa gagggtgcga cccctagcgc 10140
cgcgcttgat atgacagcgc gcaacatgga gcccagcatg tatgccagca acagaccatt 10200
cattaataaa ttgatggatt acttccatcg cgcggccgct atgaactctg attacttcac 10260
caatgctatt ctgaaccccc attggctgcc tccgcctggt ttttatactg gcgagtatga 10320
catgcctgac cccaacgatg ggttcttgtg ggacgatgtg gacagcgtgg cgttctcgcc 10380
taccgctcct cgtacttttt ggaagaagga aggtagtgac agaagaccct cctccgtgct 10440
gtcaggacgt gagggtgctg ccgcggcggt ccccgatgct gcaagcccct ttcccagtct 10500
gccattttca ctaaacagcg tgcgcagtag cgagctgggg agaataaccc gccctcgctt 10560
gctgggcgag gacgagtatt tgaatgactc cctactgaga cccgagcggg aaaagaactt 10620
ccctaataat gggattgaaa gcctggtgga taagatgagc agatggaaga cctatgccca 10680
ggagcacaga gatgagccta gaatcttggg tcctacagta ggcacccgca gacgccagcg 10740
ccatgataga cagcggggtc tggtgtggga cgatgaggat tctgcagatg acagcagcgt 10800
gttggacttg ggcgggaggg gaggtgtggg caacccgttc gcacacttgc gtccccgtat 10860
tggacgcatg atgtaaaagt gaaaataaaa aaggaactca ccaaggccat ggcgaccagc 10920
gtgcgttcgt tctttctgtt gttgtatcta gtatgatgag gcgcaccgtg ctaggcggat 10980
cggtggcgta tccggagggt cctcctcctt cgtacgaaag cgtgatgcag caggtggcgg 11040
cggcggcgat gcaacccccc ttggaggctc cttacgtgcc cccgcggtac ctggcaccta 11100
ccgaggggag aaacagcatt cgttattcgg aactcacacc cttgtatgac accacccggt 11160
tgtacctggt ggacaacaaa tcggcggaca ttgcctcgtt gaactatcag aacgaccaca 11220
gcaacttctt gacaacggtg gtgcagaaca atgactttac ccccacggag gccagcaccc 11280
agaccatcaa ctttgacgag cgctcccggt ggggcggtca gctgaagacc atcatgcaca 11340
ccaacatgcc caacgtgaac gagttcatgt ttagcaacaa gttcagggct agggtgatgg 11400
tgtccagaac cacacctaaa gaggtgacag tcacaacaga ctatgatggt agtcaggaca 11460
tcttggaata cgagtgggtt gactttgagt taccagaagg caacttctct gccaccatga 11520
ccatagacct gatgaataat gcaattgttg ataattacct aaaagtgggt agacagaatg 11580
gggtactgga gagtgacata ggtgttaagt ttgacactag gaactttagg cttggttggg 11640
acccagtgac agagttggtc atgcctgggg tctacaccaa tgaagctttc catcctgaca 11700
tagtcctact acctggctgc ggagtggact tcactgagag ccgcctcagt aatctgctag 11760
gcattagaaa gaaacagcca ttccaggaag ggttccagat catgtatgag gatctggagg 11820
gtggtaacat ccccgccctg cttgatgtaa atgcatatga gaagagcaag gaagataata 11880
caaccaccac aaatgaagct gtggccgcgg cttcatctac tgaagccaaa gctgtggtag 11940
atgcttccac ttcaacagaa aacaccactg atgaaaaagt caccagggga gatacatttg 12000
ccacccctga acaagagaag gcagctgagg cagagtctga tattatgctt ctgtccaccg 12060
atgaaaacga aactaaaaaa caactggtta ttcgagcggt gaccaaggat agtaaggaca 12120
ggagttataa tgtattgtca gatggaaaga acacagctta ccgtagttgg tacctggcat 12180
acaattatgg cgaccgtgag aaaggggtgc gttcttggac actgcttacc acctcggatg 12240
tcacctgcgg cgtggagcaa gtctattggt cgctaccaga tatgatgcaa gatccagtca 12300
cctttcgctc cacacgccaa gttagcaact acccagtggt gggcgcagag ctgctcccag 12360
tgcattccag aagcttctac aacgagcaag ccgtctactc gcaacagctc cgccagtaca 12420
cctcgctcac gcacgtcttc aaccgcttcc ccgagaatca gatcctcgtc cgcccgcccg 12480
cgccaaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac gggaccctgc 12540
cgctgcgcag cagtatccgg ggagtccagc gcgtgaccgt tactgacgcc agacgccgca 12600
cctgtccata cgtatacaag gccctgggca tagtcgcgcc gcgcgtcctt tcaagccgca 12660
ctttctaaaa aaatgtccat tctcatctcg cccagtaata acaccggttg gggcctgcgc 12720
acacctagca agatgtatgg aggcgctcgc agacgctcca ctcagcaccc tgtgcgcgtg 12780
cgcgggcatt tccgcgctcc ctggggcgcc ctcaagggac gctctcgtac taggaccacc 12840
gttgacgatg tgatcgacca ggtggtcgcc gatgcacgta actatacccc cgcagccgca 12900
cctgcatcca ccgtggatgc ggtcattgac agcgtggtag ccgatgcgcg cgcctatgct 12960
cgcgccaaga gcaggaggcg gcgtattgcc aggcgtcacc gagctactcc agccatgcga 13020
gctgcaagag ctttattgcg gagagccaga cgtgtggggc gaagagccat gcgtagagcg 13080
gccagacgcg cggcttcagg tgccagcgca ggcagggtcc gcaggcgcgc ggctacggcg 13140
gcagcggcgg ccatcgctag catgaccaaa ccacgaagag gcaatgtgta ttgggtgcgc 13200
gacgccgcca ccggccagcg cgtgcccgtg cgcacacgcc cccctcgcac ttagaagata 13260
ctgagcagtc tccgatgttg tgtcccagcg gcgagatgtc caagcgcaaa ttcaaggaag 13320
agatgctcca ggtcatcgcg cctgagatct acggtcctgc ggtgaaggat gaaaaaaagc 13380
cccgcaagat caagcgggtc aaaaaggaca aaaaggaaga agatggtgat gatgggctgg 13440
tggagtttgt gcgcgagttt gccccaagga ggcgcgtgca gtggcgcggg cgcaaagtgt 13500
ggccggtgtt gagaccgggg accacagtgg tctttacgcc aggcgagcgc tccagcaccg 13560
tttccaaacg ctcttatgat gaggtgtacg gggacgatga tattctcgag caggcggctg 13620
atcgccttgg cgagtttgca tatggcaaac gcagccgctc gggagccaag gaagaggcat 13680
tgaccatccc cttggatcat ggaaatccca ccccaagcct caaacccgtg accctgcaac 13740
aagtgctgcc cacgccgcca cgcaagggca tcaagcgcga gggcgaggat ctgtatccca 13800
ccatgcagct gatggtgccc aagcgccaga agctggaaga cgtgctggag aaaatgaaag 13860
tggatcctga aatccagcct gaagtcaaag tgaggccaat caagcaggtg gcgcccggtt 13920
tgggggtaca aaccgtggat atcaagatcc ccaccgagtc catggaaatt caaaccgaac 13980
ccatgaagcc cacctccagc accattgagg tgcagacgga tccttggatg cccgcgcctg 14040
ctcctgttac cactactact cgaagaccta gaagaaagta tggttcagcc aacctgataa 14100
tgccaaacta tgctctgcat ccatcaatca tacccactcc tggctaccgc ggcactcgct 14160
actaccgcag tcacagcacc cgccgacgta aagcacctgc cacccgccgc cgtcgccgcc 14220
gccgtgccac tagcaaactt accccctcgg ctatggtgcg gagagtgtac cgtgatgggc 14280
gcgcagctcc tctgacactg ccgcgcgcgc gctaccatcc tagcattgcc atttaacaac 14340
tctgcctcct tgcagatatg gccctcactt gccgccttcg tattcctatt gctggctacc 14400
gcggaagaaa gtcgcgccgt agaagagcag ggttgtctgg gagcgggatg cgtcgccacc 14460
ggcggcggcg cgccatcagc aaacggttgg ggggtggatt tcttcccgct ttgattccca 14520
tcatcgccgc ggcgatcggc gcgataccag gcatagcttc cgtggcggtg caggcctcgc 14580
agcgccactg acattggaaa aagatatctt ataaataaaa atagaatgga ctctgacgct 14640
cctggtcctg tgatatgttt ttgtagacga gatggaagac atcaattttt catccctggc 14700
tccgcgacac ggcacgcggc cgtatatggg cacctggagc gacatcggca acagccaact 14760
gaacggggga gccttcaatt ggagcagtct atggagcggg cttaaaaatt ttgggtccac 14820
tataaagact tatgggaaca aagcttggaa cagcagcaca gggcatgcgc tgagacaaaa 14880
gcttaaagat cagaatttcc aacagaaggt ggtcgatggt atcgcctctg gaatcaatgg 14940
ggtggtagat ctggccaacc aggccgtgca gaaacagatt aacagtcgcc tggacccggc 15000
tcccccagct cctattcatg agttaatgca agtggaggaa gagctccctt cattggaaaa 15060
gcggggcgat aagcgacctc gtccagatat ggaggaaacg ctgctgacca aggtggatga 15120
gccgccctcc tatgaagagg ctgtaaaact gggaatgccc actacaaagc ccattatgcc 15180
tctggccact ggagtgatga agccatctca gtctaaacct gcagttgctg ctacattgga 15240
cttgcccgct cccgtggcca cccccaaacc tgtcgccgcc ccgaagccca ccgccgtgca 15300
acccgtggcc gtggccagac cgcgtcccgg tggtcggccg aatgcaaact ggcagagcac 15360
tctgaacagc atcgtgggtt tgggagtgca cagtgtgaag cgccgtcgct gctattgatt 15420
aaatatggag tagcgcttaa cttgcttgtc tgtgtgtgta tatgtcgatg ccgcccgccg 15480
tgctacagca aagagagaag gagaagaggc gccgctgagt tcctttcaag atggccaccc 15540
catcgatgct gccccagtgg gcgtacatgc acatcgccgg acaggacgct tcggagtacc 15600
tgagtccggg tctggtgcag ttcgcccgcg ccacagatac ctacttcaat ctggggaaca 15660
agtttaggaa ccctaccgtg gctcccaccc acgatgtgac caccgaccgt agccagcgcc 15720
tgacgctgcg ctttgtgccc gttgaccggg aggacaatac ctactcctac aaagtcagat 15780
acaccctggc tgtgggagac aacagggtgt tggatatggc cagcacctac tttgacatca 15840
ggggcgtgtt ggacagagga cctagcttca aaccatactc tggcactgcc tacaactccc 15900
tggctccaaa aggagctcca aactccagtc agtggcaaca aaaggaaaac aatggtcaag 15960
gtgatgcaaa gactcacacc tatggtgtag ctgccactgg aggtattgac attgacaaaa 16020
atggtcttca aattggaatc gatgaaacta aagaagataa taacgaaatt tatgcagaca 16080
aaacattcca acctgaacct caaattggag aagaaaactg gcaagatagc gaaaactatt 16140
atggaggcag ggctcttaaa ccggaaacca agatgaagcc ttgctatggt tccttcgcta 16200
gaccaactaa tgcaaaggga ggtcaagcca aaattaaacc agctcaagag ggtcaacagt 16260
ctatagatta tgacatagac ctggctttct ttgatattcc aagcactggc ggaggcaatg 16320
gcacaaatgt aaatgacaag ccagatatgg ttatgtatac tgaaaatgta aatctggaaa 16380
ctccagacac tcatcttgtt tacaagccag gaacttcaga tgacagttcc gaggccaatt 16440
taactcagca agccatggct aacagaccca actatattgg gtttagagat aactttattg 16500
gcgtcatgta ctacaacagc actggcaaca tgggagtgct tgctggtcaa gcatcccagc 16560
taaatgctgt ggtggacctg caagacagaa acaccgagct gtcttatcag ctattacttg 16620
actctctggg cgacagaacc aggtatttta gtatgtggaa tcaggcggtg gacagctatg 16680
atcctgatgt gcgcattatt gaaaaccatg gtgtggaaga tgaattgcca aactattgct 16740
tcccattgga cggagctggc actaatgctg tttaccaagg agttaagaca aaagaggata 16800
ataatggcga atgggaaaca gacacaaatg ttgcatcgca gaatcagata tgcaagggca 16860
acatatatgc tatggagatc aacctgcaag ccaacctgtg gaaaagtttc ctttactcca 16920
acgtggctct gtacctacca gactcctaca agtacactcc atccaacgtg acactcccta 16980
ccaacactaa cacctatgac tacatgaatg gcagggtggt gtctccatcc ctggtggatg 17040
cctacattaa cattggcgcc aggtggtctc tggatgccat ggacaatgtc aaccctttca 17100
accaccaccg caatgccggc ctgcgctacc ggtccatgct tctgggcaac ggccgatacg 17160
tgcccttcca catccaagtg ccccagaaat tcttcgctat caagaacctg ctgcttctcc 17220
caggctcata cacctacgag tggaacttcc gcaaggatgt caacatgatc ctgcagagtt 17280
cccttggcaa tgacctcaga accgatgggg ccaccatcca gtacaccagc atcaatctct 17340
atgccacctt cttccccatg gctcacaaca ctgcctccac cctggaagcc atgctgcgca 17400
atgacaccaa tgaccagtcc ttcaatgact acctctcagc tgccaacatg ctttacccca 17460
tccctgccaa tgccaccaac gtgcccatct ccatcccatc tcgtaactgg gctgccttca 17520
ggggctggtc tttcacccgc ctcaagacca aggagacccc atctctggga tcagggttcg 17580
atccctactt cgtctactca ggctccattc catacctgga tggaactttc taccttaacc 17640
acactttcaa gaaagtctcc atcatgtttg actcttctgt cagctggcca ggcaatgaca 17700
ggctgctgac tcccaatgag ttcgaaatca agcgcactgt tgatggggaa gggtacaatg 17760
tggcacaatg caacatgacc aaagactggt tcctggttca gatgctctcc cactacaaca 17820
ttggctacca gggcttctac atcccagaag gatacaagga ccgcatgtac tccttcttca 17880
gaaacttcca gcccatgagc cgccaggtgg tcgatcaggt caactacaaa gactacatgg 17940
cagtcaccct tgcctatcag cacaacaact ctggctttgt gggctacctc gcgcccacca 18000
tgcgacaggg ccaaccctac cctgctaact acccataccc gctcattggc aagactgcag 18060
tcaacagtgt cacccagaaa aagttcctct gcgacagggt catgtggcgc atccccttct 18120
ccagcaactt catgtccatg ggggccctta ccgacctggg gcaaaacatg ctttatgcca 18180
actccgccca cgcgctagac atgaatttcg aagtagaccc catggatgag tccacccttc 18240
tctatgttgt cttcgaagtc ttcgacgtgg tcagagtgca ccagccccac cgcggcgtca 18300
tcgaagctgt ctacctgcgc acccccttct cagctggtaa cgccaccaca taagcgcctt 18360
gcttcttgca agtggctgca gcagcatggc ctgtggatcc tccactggat ccaatgagca 18420
agagctcagg gccatcgcca tagacctggg ctgtggaccc tatttcctgg gaacctttga 18480
caagcggttt ccaggcttca tggctcctga caagctcgcc tgtgccattg tcaacacggc 18540
agggcgcgag actggtggtg agcactggct ggcttttgga tggaaccccc gctccaatac 18600
ctgctatctc tttgacccgt ttgggttttc agacgagcgc ctcaagcaga tctatcaatt 18660
cgagtacgag gggctcctgc gccgcagtgc cctggctact aaggaccgat gcatcactct 18720
ggaaaagtct acccagaccg tgcagggtcc gcgctcggct gcctgcgggc tcttctgctg 18780
catgttcctc catgcttttg tgcactggcc cgaccgcccc atggacaaca accccaccat 18840
gaatttgctg acgggggtac ccaacaacat gctccaatcg ccccaagtag agcccaccct 18900
gcgccacaac caggaggcac tctatcgctt cctgaactcc cactcatctt actttcgttc 18960
taaccgcgcg cgcattgaga aggccactgc cttcgatcga atgaataata acatgtaaac 19020
caaattgtgt gtggctcaaa taaacagcac tttattgttt acatgcactg aggctctggg 19080
atgatcattt tttaaaaatc gaaggggttc tggcgggaat cagcatggcc agatggcagg 19140
gacacgttgc ggaactggaa cttgttctgc cacttgaact cgggaatcac cagcctggga 19200
actggaatct ctggaaaggt atcttgccat agctttctgg tcagttgcag agcgccaagc 19260
aggtcaggag cagatatctt gaaatcacag ttggggccag aattctgggc gcgggagttg 19320
cggtacactg ggttgcagca ctggaacacc ataagggcag ggtgtctcac gctcgccagc 19380
acggtctcgt cactgatgca agacacatcc aggtcttcag cattggccat tccaaagggg 19440
gtcatcttgc aggtctgtct gcccatcacg ggagcgcagc caggtttgtg gttgcaatca 19500
caatgaaggg ggatcagcat catcttggcc tggtcggggg taatccctgg gtaaacagcc 19560
ttcatgaagg cttcatactg cttgaaagct tcctgggctt tggttccctc ggtgtagaac 19620
actccacaag acttgctgga aaactgatta gtagcgcagt tggcatcatt cacacagcag 19680
cgggcgtcgt tattagccag ctggaccaca ttcctgcccc agcggttctg ggtgatcttg 19740
gctcgatctg ggttctcctt caacgcgcgc tggccgttct cgctcgccac atccatctca 19800
atgacatgtt ccttctggat catgatgttg ccatgcaggc atctaatctt gccttcataa 19860
tcagtgcagc catgaggcca cagcgcgcac ccggtgcact cccaattgtt atgggggatc 19920
tgggaatggc tatgaaccag cccttgcagg aatcttccca tcatcacagc cagggtcttt 19980
atgctggtaa aggtcagcgg gataccgcgg tgctcctcgt tcacatactg ctggcagatg 20040
cgtctgtagt gctcggcctg ctcgggcatc agcttgaaag aggttttcaa ctcattatcc 20100
agcctgtatc tctccatcat gatggacatt acttccatgc ccttctccca ggcagaaaca 20160
atagggagac tcaggggatt cttgacagta gagacaacct tacttaaggg gtcatcactg 20220
ccaatctttt cgatgcttct cttgccatcc ttctcggtga tgcgcaccgg cgggtagctg 20280
aatcccacag ccaccaactg agcctcttcc ctttcgtctt cgctgtcttg actgatgtct 20340
tgcagaggaa catgtttggt tttcctgggt ttcttcttgg gcggcagctc tggaggactc 20400
tggctccgtt ccggagaccc catggatgag cgagagttgt cgctcaccac ttggatctgg 20460
ctgcctgtag aagaactgga ccccacgcgg cggtaggtgt tcctcttggt aggcagaggt 20520
ggaggcgacg ggctccggtc cggtctgggt ggcggatggc tggcggagcc ccttccgcgt 20580
tcgggggtgc gctccagatg gcggtcgtct gactgacctc cgcggctggc cattgtgttc 20640
tcctaggtag agaaacaaga catggagact cagccatcgc tgccatcgcc atccaccacc 20700
acaagcaccg ccgaggagga ggagtgttta accaccccac catgcagccc cgctaccacc 20760
accagcaccc ttgaaagcga ggtcgacacg gtcgtggagg atttacaggc tatggaagat 20820
attgaggcag ctgtcgagca agaccccggc tatgtgacac cggcggagca tgatgaggat 20880
ctagcgcgct ttctcgacgg tgtggagaaa gcgaaacaag atgaggacga ggaagaggca 20940
gaagcacaac catcggtggc cgactacctc accggcctag ggctagaaga cgtgctgctt 21000
aagcatcttg caaggcagac agtcatagtc aaagacgccc tgctagagcg ctccgaggtg 21060
ccactcagtg tggaagacct cagtcgcgcc tatgagctaa acctcttctc gcctcgcaag 21120
ccccccaagc gtcagcccaa cgggacctgt gagcccaatc cgcgcctcaa cttctatcca 21180
gccttcactg tgcccgaagt actagctacc taccacatct ttttcaagaa ccaaaagatc 21240
cccatctcct gccgcgccaa tcgcacccgc gcagatgccc tactcaactt ggggcccggc 21300
gctcgcatac ctgatatcgc ttccttggaa gaggttccta agatctttga gggtctgggc 21360
aatgaggaaa ctcgggcagc aaacgctctg caaagagaaa cagatgatgg tgaacaccac 21420
agcgctctgg tggagctcca gggcgacaac gctcgtcttg cagtcctcaa acgcagcatc 21480
gaggtcaccc atttcgccta ccccgcactt aatctcccac ccaaagtcat gagctcggtc 21540
atggacacgt tgctcatgaa gcgcgcgagc cccatctccg aggatcagaa catgcaggac 21600
cccgatgcct cagatgaagg caagcctgta gtcagcgacg agcaactggc tcgctggcta 21660
ggctctgact ccccccagtc tttggaggag cggcgcaagc ttatgatggc agtggtcctg 21720
atcacagcgg agctggagtg tctccgccgc ttcttcactg acccagagac cctgcgcaag 21780
cttgaggaga acctgcatta cacattcagt catgggttcg tgcgccaggc gtgcaagatc 21840
tccaacgttc aactcaccaa cctggtctcc tacctgggca tcttgcatga aaaccggctg 21900
gggcagaacg tgctccacac caccctgaag ggggaggccc gccgcgacta tatccgcgac 21960
tgtatctacc tctacctatg ctacacctgg caaagcggga tgggtgtgtg gcaacagtgc 22020
ttggaagagc aaaatctaaa agagctggaa aagctgcttc agaaatctct taaatctctg 22080
tggaccgggt tcgatgagcg gaccaccgct tcggacatgg ccgatattat cttccccgag 22140
cggctcagac acactctgcg cgacgggctg cctgactttg ccagccagag catgctacaa 22200
aactttaggt cattcatctt ggaacgctcc gggatcctgc ccgccacttg ctgcgcactg 22260
ccctccgatt ttgtgcccat cacctaccgg gagtgccccc cgccgctatg gagccactgc 22320
tacctgttcc gcctggccaa ctacttggcc taccactctg atgtgataga agatgttagt 22380
ggcgaagggc tcctggagtg ccactgccgc tgcaacctct gcacccccca ccgctccctc 22440
gcctgcaatc cccagctgct gagcgaaacc cagatcatcg gcaccttcga gttgcaaggt 22500
cccagcggcg aaggcgaggg gtcctctccg gggcaaagtc tgaaactgac tccggggcta 22560
tggacctccg cttaccttcg caagttcgcc cccaaagact accaccccta tgagatcagg 22620
ttttatgaag accaatcaca gccccccaag gccgaactga cggcctgcgt catcacccag 22680
ggggcaatct tggcccaatt gcaagccatc caaaaatccc gccaagaatt tttgctgaaa 22740
aagggacacg ggatctatct agacccccag accggtgagg agctgaatac acgcttccct 22800
caggatgccc cgaggaggca agagaatgaa agttcagatg ccgcccgagg aggagctgga 22860
agactgggac agtcaggcag aggaggaaga ctgggacagc caggcagaag aggaggacag 22920
cctggaggag gacagtctgg aggaaggcga ggagcccaag gaagaggcag ccgccgccag 22980
accatcgtcc tcggcggtgg agacaagcaa ggtcccagac agcacggcta ccacctccgc 23040
tccagctcaa ggggccgctc ggcgacccaa cagtagatgg gacgagacgg gtcgcttcca 23100
gaaccccacc accgtcaaga ccggtaagca ggagcggcag ggatacaagt cctggcgggg 23160
gcataaaagt gccatcatcg cttgcttgca ggagtgtggg ggcaatatat cctttgccag 23220
acgctacctg ctattccatc acggggtgaa tttcccccgc aacatcttgc attactaccg 23280
tcacctccac agcccctact accagcagca agagacagca gaggaaacca gcggcaactc 23340
cgagagttag aaaaccagca gctaaaaaat ccacagcggc ggcagcaggt gcaggcggac 23400
tgaggatcac cgcgaacgag ccagctcaga ccagggagtt gaggaatcgg atctttccca 23460
ccctctatgc catattccaa caaagtcggg gtcaggaaca agaactgaaa gtaaaaaaca 23520
gatctcttcg ctcgctcacc cgcagttgtt tgtatcacaa gagcgaagac caacttcagc 23580
gcactctcga ggacgccgag gctctcttca acaagtactg cgcgctgact cttaaagagt 23640
agactgcgcg cgcttggcga gaaaaggcgg gaattacgtc acctcttggc cacacctgtg 23700
cttcattatg agtaaagaaa ttcccacgcc ttacatgtgg agctatcagc cccagatggg 23760
attggccgct ggcgccgccc aggactactc cacccgcatg aattggctca gcgccggtcc 23820
cgcgatgatc tcacgggtta atggtgtgag agagcaccga aaccagatac tcctagaaca 23880
gtccgccctc accgccactc cccgcaatca cctcaacccc cgtaattggc ccgccgccct 23940
ggtgtaccag gaaactcctg ctcccactac agtactactt cctcgtgacg cccaggccga 24000
agttcagatg actaactcag gtgtacagct ggcgggtggt gccaccctgt gtcgtcaccg 24060
gccaagaccg ggtataaagg gcctggtgat cagaggccga ggtattcagc tcaacgacga 24120
gtcggtgaac tcttcgcttg gtctgcgacc agacggcatc ttccaaatag ctggttgtgg 24180
gagatcttcc ttcactcctc gtcaggctgt cctgactttg gagagttcgt cctcgcagcc 24240
ccgctcgggc ggcatcggga ctctccagtt cgtggaggag tttactccct cggtctactt 24300
caaccccttc tccggttctc ctgggcttta cccggacgag ttcatcccga actacgacgc 24360
catcagtgaa gcggtcgacg gctacgatta atgtctaatg gtggcgcggc tgagctagct 24420
cgactgcgac acctagacca ctgccggcgc tttcgctgct ttgctcggga tctctgcgag 24480
ttcatctact tcgagtaccc tgacgaacat cctcagggac ctgcccacgg agttcggatt 24540
accattgaag gggctatcga ttctcacctg cttcggatct tcaccgctcg gccagtgcta 24600
gttgagcgca accagggcga caccaccatc tccctctgct gcatttgtga caaccccgga 24660
ttgcatgaaa gcttttgttg tcttctttgt actgagtata ataaaagctg aaattagaga 24720
ctactccgga ctctcttgtc gtctgaacaa caccaaccag acccttcact tcagcgggaa 24780
ccagactact cttcactgta aggcttataa ctataagtat cttacttgga tatacaaagg 24840
aacaccgttt gctgtggtaa acaggtgctc caacgacggt gttctcctca ccttcctagg 24900
caacttctcc aactttacct tttctgttcg cagaaacaag cttaccctcc ttcagcccta 24960
ctttcctggg atctatacct gcctcagtgg accttgcaac cacacttttc acctgattga 25020
aaactctacc cttaccttcc cagcgccaat ccctactaac agctcggagt ccaactcttc 25080
cattaccgct gatactaaca ctcctaaaac cggaggtgag ctccgcagcc ttcccccggc 25140
tgcagataac ccttgggtgg tagcgggatt tgtagcgcta ggaatagttg cgggtgggct 25200
cgcgttcgtc ctctgctacc tataccttac ctgctgctca tatttagtag tactgtgctg 25260
ttggtttaga aaatgggggc gctactaatc acacttgctt tactttcgct tttgggtctg 25320
agctcggcta atagcgagaa accaagctgt ctagaaacaa actctccaga ctgtgtggtt 25380
cctcatgggc tctcagaccc agctgatgat ccatgcttaa cttttgaccc agaaaaaaac 25440
tgctcggtga ctatgcagcc ctatgcttac atgtgcacat ctgttataaa gtgcggatgg 25500
ggctgtaaac cgattgaaat tacccacaaa ggcaaaacct ggaataatag tttgtttaac 25560
acatggcagc ctggagacga gcagtggtat acggccggcc actggtggag atgactgacc 25620
ccatggaaaa ctcctctgcc aacgacctgg acatggacgg ccgttcatct gagcagcgac 25680
tggtccagat gcgcattcgc cagaagcagg aacgcgccgc cagagagctc aaggatgcca 25740
ttgaaattca cctgtgcaag aagggcatct tttgcttggt taagcaagca aagatttctt 25800
atgaaatcac tgacaacgac caccgcctgt attatgagct cggtccacag cggcagaaat 25860
tcacctgcat ggttggagtc aaccccatag tcatcactca gcaggctgca gaaattaaag 25920
ggtgcatcca ctgttcctgt gattcccaag aatgcgtcca caccatagtc aagaccctct 25980
gcggccttcg agatcttctt ccaatgaact aaccccttcc cccaacccaa taaaacattg 26040
gttttaatca taataaaaaa tcacttactt taaatctgaa acagtgtctc cgtccaagtt 26100
ttcttgtagc accacttcac tcccctcttc ccagctctgg tactgcaagc cccggtgggc 26160
tgcaaacttt ctccacacct taaaagggat gtcaaattcc tcttgtccaa caatcttcat 26220
tgtctcttcc tagatgtcca caaagcgcgc gcgggtggaa gatgactttg accctgtcta 26280
cccatacgat gctgagctgg caccgtctgt acccttcatc gcccctccct tcgtttcgtc 26340
agacggattt caagaaaaac ccctgggagt tctgtcccta agactagcca acccagtcac 26400
tactaaaaat ggggaactca cacttaaact gggagatggg gtgggcatag actcagatgg 26460
aaacctcaca gcacagacag ttactaaagc aacatccccc cttactgttt ccaataacgc 26520
aattgcactt aacatggaca aaccttttta cagtagcaat ggaaaactat ccttacaagt 26580
tacatcacca ttaaagatag tcgactcttt aaatacattg gctattggct atgggcaagg 26640
cttaggacta aacaatagtg ctcttgctgt gcaattagca tctcccctta catttgacag 26700
caacagcaaa attaaaataa atttgggaag cgggccatta aaaattaatg cgaataaact 26760
gtcaattaac tgcctaaggg gtgtatatgt aacaactgac ggaacttcca ttgaaacaaa 26820
tataagctgg gcaaaaggaa tgaggtttga aggtaatgcc atggctgtaa acgttgacag 26880
caccaaaggt ctacaatttg gcactaccag cacagaatca ggagtcacta acgctttccc 26940
tatccagtta aagattggat ctggtcttag ttttgacagc acaggagcac ttgtagcttg 27000
ggataaggat aatgacaagc ttacactgtg gacaaccgct gacccatcac ctaattgtac 27060
catatataca gacaaggatg ctaaacttac actttgtctt acaaaatgtg gcagtcaaat 27120
actaggcagt gtttcagtac tggctgttaa agctggaacc ctacagccaa tcagtgaaaa 27180
aataggtact gctttggttt cactaaaatt taataacaac ggtgtattgt taagcaactc 27240
cacattaagt aatgaatact ggaactacag gaagggagat gtcacaccag ccgaagccta 27300
tactaatgct gtgggtttta tgccaaacat caaggcatat cctaaaaaca caaactctgc 27360
ctcaaaaagc cacattgtag gacaagtgta ccttaatgga gatgaaacta aaccaatgca 27420
tttaatcatt acatttaatg aaaccagtga tgaaacatgc acatattcca taacgttcca 27480
atggaaatgg aacattggaa catacaccag cgacaccctt gcaacaagct cctttacctt 27540
ttcttacatt gcccaagaat aaaaactgca gacaacaata aagtttaaat gttttattta 27600
aacagtttca cagaacccta gtattcaacc tgccacctcc ctcccaacac acagagtaca 27660
cagtcctttc tccccggctg gccttaaaaa gcatcatatc atgggtaaca gacatattct 27720
taggtgttat attccacacg gtttcctgtc gagccaaacg ctcatcagtg atattaataa 27780
actccccggg cagctcactt aagttcatgt cgctgtccag ctgctgagcc acaggctgct 27840
gtccaacttg cggttgctta acgggcggcg aaggagaagt ccacgcctac atgggggtag 27900
agtcataatc gtgcatcagg atagggcggt ggtgctgcag cagcgcgcga ataaactgct 27960
gccgccgccg ctccgtcctg caggaataca acatggcagt ggtctcctca gcgatgattc 28020
gcaccgcccg cagcataagg cgccttgtcc tccgggcaca gcagcgcacc ctgatctcac 28080
ttaaatcagc acagtaactg cagcacagca ccacaatatt gttcaaaatc ccacagtgca 28140
aggcgctgta tccaaagctc atggcgggga ccacagaacc cacgtggcca tcataccaca 28200
agcgcaggta gattaagtgg cgacccctca taaacacgct ggacataaac attacctctt 28260
ttggcatgtt gtaattcacc acctcccggt accatataaa cctctgatta aacatggcgc 28320
catccaccac catcctaaac cagctggcca aaacctgccc gccggctata cactgcaggg 28380
aaccgggact ggaacaatga cagtggagag cccaggactc gtaaccatgg atcatcatgc 28440
tcgtcatgat atcaatgttg gcacaacaca ggcacacgtg catacacttc ctcaggatta 28500
caagctcctc ccgcgttaga accatatccc agggaacaac ccattcctga atcagcgtaa 28560
atcccacact gcagggaaga cctcgcacgt aactcacgtt gtgcattgtc aaagtgttac 28620
attcgggcag cagcggatga tcctccagta tggtagcgcg ggtttctgtc tcaaaaggag 28680
gtagacgatc cctactgtac ggagtgcgcc gagacaaccg agatcgtgtt ggtcgtagtg 28740
tcatgccaaa tggaacgccg gacgtagtca ttctcgtact ttgagtggca aaaccttgct 28800
ctcgaacagc acacgtctcg tcgcctcctg tcccttctct tcgccttttc agtgtgatag 28860
ttgtaataca gccattcacg aagctcagtc agaagatctt cagcgtctgt tgtcaaaaac 28920
aatccatcca atctgattgc tttcaaaaca tcacaaacag tcgaataagc caaacccatc 28980
caggcaatgc aattattttg gttatccaca atgggagggg gcggaagaca tggaagaggc 29040
ataattaatt ttttaatcca atcgatcacg cagcacttca aaatgaagat cgcgaaggtg 29100
acacctttca cccccactgt gttgatgaaa aataacagcc aagtcaaaat tgatgcggtt 29160
ttcaaggtgc tcgactgtag catcaagcag agcttccaca cgcacgtcca caaataacag 29220
aatagcaaaa gcgggaggag gaagtaaatc ctcaatcatc atagtacagt ccatcaccat 29280
ccctaaataa ttttcatcct tccagccttg gactatattt ttaaactgct cttgtaaatc 29340
caaaccacac atgtggaaaa gttcccaaag agctccctca actaccattc ttaagcacac 29400
cttcatagtg acaaaatatc ttgttcctct gtcacctgca gcaaattaca aagtccaata 29460
ttaggatcta tgcccagaga tctaagctca tccctcaatt ccaactgtaa aaaggcttcc 29520
agatctgccc taacttgttc agccagtggg ctccctggaa taagcgtggg agaagccaaa 29580
ctgcaaaaca gacgcatgcc gccataatta ccaccagaaa acactacgtt acagtatgca 29640
tgctgattca ttccagtaat ttcatccagt gtattggata caaaaaaagg caagcactct 29700
ctcactaatt gtattatgga gacattatca cacaggtaac aatttaaagg ttgtggaaca 29760
ataatgcagt aagtaaccac ggtgcgctcc aacatggtta gtaattttta gttctgaaaa 29820
acaaaacata caaaaaatta tatcatactc atttggcgaa ctggtggaaa aatgacccta 29880
tctagcacaa ggcaagccac tggatcacca atgcgcccct cataaaacct gtcatcatga 29940
ttaaaaagca acaccgaaag ctcttcccta tgtcctgcat gaatgattct agctgaggaa 30000
tataagccag cgcaattagt atctgttaaa gaaaaaaaac ggccaacata gcctctagga 30060
attagcacac ttaatcttaa agacattact gccatccccc ttggatttaa ggtaaaattt 30120
acaggagcat agaaaatata ctgatttccc tcctgcacag gcagcatagc accaggtccc 30180
tctaaaaaca cacacaaagc ttctgcagcc atagcttacc gcgcaaacca ggcacagcag 30240
tgagctaaaa ggacaaagct ctaactcact agccaacctg gcgcacaata tatagttagt 30300
ccttacactg acgtaaccga ccaaagtcta aaaaccccgc caaaaataca cacacgccca 30360
aaaaacgccc cgtgagtcaa aaaacagttt cacttcctcg ttacacccaa aacgtcgtca 30420
cttccggatt cccacggttc gtcacttccg gagctccttg cttaattaac cccgcccaaa 30480
acgtcatcgt ccgcgtcacg ccgccccgcc ccgcgaccgt tgaccccggg ccaatcaccg 30540
cacatcccgc aaaattcaaa ctcgtctaat ttgcatattg gcacactgcc catataaggt 30600
atattattga tgatg 30615
<210> 15
<211> 3586
<212> DNA
<213> 人工序列
<220>
<223> BLY6载体左端
<400> 15
caattgattt aaatcatcat caataatata ccttatatgg gcagtgtgcc aatatgcaaa 60
ttagacgagt ttgaattttg cgggatgtgc ggtgattggc ccggggtcaa cggtcgcggg 120
gcggggcggc gtgacgcgga cgatgacgtt ttggggagga ggagctatgt tgcaagtaat 180
cgtgggaaat gcgacgtaaa acgaggtgga gtttaaacac ggaagtagac aattttcccg 240
cgctgtttga caggaaatga tgtgtttttg ggcggatgca agtgaaaatt ctccattttc 300
gcgcgaaaac taaatgagga agtgaaattc tgagtaattc tgaggttatc acagggcgga 360
gtatttaccg agggccgagt agactttgac ccattacgtg gaggtttcga ttactctatt 420
tttcacctaa atttccgcgt actgtgtcaa agtccgtgtt tttacgcgat cgcgatggcc 480
tgtgtttgag cataatgtac tgaccaggtg taacgttcat ctgggtggtc gtagaggaat 540
gttcatgcca taccaatgca attttaatca tgtgaggatc ttgatggagc cgcaagcgtt 600
ttccagagtc agcttgactg gaatctttga catgtgtgtg gaagcatgga agatcttaag 660
atatgatgat accaaatcca gatgccgcgc atgcgagtgc gggggcaggc atgccaggtt 720
ccaacctgta tgtgtggagg tgaccgagga gctgagacca gatcatttgg tgctgacctg 780
cactggtgcg gagttcggtt ccagtggtga agaaactgat taaagtgagt agtgggatgt 840
tataaaagtg accataaggt gatgtgagat ggacaaattt ggtaattttt atgtattttt 900
gtcttgcagc catgagtggg agcgcttcct ttgaaggggg cgtctttagc ccttatctga 960
cggggcgtct gcctcattgg gctggagtgc gtcagaatgt gatggggtct acagtggatg 1020
gaagacctgt tcagcctgct aattcttcta ctctgactta tgctactatg acttcctcgc 1080
ctttggatgc agctgcagct gctgccgctt ctgctgccgc caacactgtt cgggggatgg 1140
ccttggagat ggggtattat ggaactgtag tggccaacac cactacccca aataacccca 1200
cagccttgaa tgaggacaag ctgctagttc tcatgtccca gctggagtct ttgacccaac 1260
gcctgggcga tctagctcag caggtgtccc agctgaagga gcagactcaa gctgcaatta 1320
ccactgcgag gggaaattaa aaaaattcaa agaatcaata aataaaccga gactttgttg 1380
attttaaagt gtgtcattct ttatttaatt tttcgcgcgc gatatgccct ggaccaccgg 1440
tctctatcat tgaggacacg gtggatcttt tctagaaccc gatagaggtg ggattggatg 1500
ttgaggtaca tgggcataag accatctttg gggtgtagat agctccactg cagagcctca 1560
tgctccgggg tggtgttgta tataacccag tcatagcatg ggcgttgggc atgatgttgc 1620
acaatatctt taaggaggag actaatggcc actgggagac ccttggtgta agtgtttaca 1680
aatctattaa gctgggacgg gtgcatccga ggtgagataa tgtgcatttt ggattggatt 1740
tttagattgg caatgtttcc ccctagatct ctcctgggat tcatgttatg caagaccact 1800
agaacagtgt atccggtgca cttagggaat ttgtcatgaa gtttggaggg gaaagcatga 1860
aaaaatttag acacaccctt gtgtcctccc aagttctcca tgcactcatc cataataatg 1920
gcaatgggcc catgggcggc ggcacgggcg aacacgttcc tgggatctga cacatcatag 1980
ttgtggtctt gggtcaggtc atcataagcc attttaataa acttggggcg gagggtgcca 2040
gattggggga tgaatgttcc ctcgggcccc ggaacatagt ttccttcaca tatttgcatt 2100
tcccaggctt ttagttcaga gggggggatc atgtccacct gtggagcgat gaagaagacg 2160
gtctcggggg cgggggtgat taagtgggag gacagcaagt tcctaagcag ctgtgacttg 2220
ccacacccag tgggaccgta gatgacccct ataacaggtt gcagatggta gtttagggaa 2280
agacagctgc cgtcctctcg caggaggggg gcgacctcgt tcatcatttc cctcacatgc 2340
atgttttccc gcacaagttc cgataggagg cgctctccac ccagggaaag gagttcttga 2400
agagatgaga aatttttcaa gggttttaag ccatcagcca tgggcatttt ggagagggtt 2460
tgttgcaaga gttcaaggcg gtcccagagt tcggtgatgt gttctatggc atctcgatcc 2520
agcatacttc ctcgtttctg gggttgggac ggctgcggga gtatggaacc aggcgatggg 2580
cgtccagcgc tgccagtgtc cggtccttcc acggtcgcag cgtccgagtc agggtcgttt 2640
ccgtcacggt gaaggggtgc gcgcctggct gggcgcttgc gagggtgcgc ttcaggctca 2700
tcctgctcgt ggagaaccgc tgccgttctg cgccctgtgc atcggccagg tagcaattaa 2760
ccatgagttc gtagttgagc gcctctgccg cgtggccttt ggcgcgcagc ttacctttgg 2820
aagtcttctg acaggtggga cagtagagac acttgagagc atagagtttt ggggctagaa 2880
agaccgattc tggggagtat gcatcggccc cacaggaggc gcagacggtt tcgcattcca 2940
ccagccatgt aagatcgggc tcgttggggt caaaaacaag ttttccgcca tgttttttga 3000
tgcgtttctt acctttgctt tccatgagtt cgtgcccccg ttgggtgaca aagaggctgt 3060
ccgtgtcccc gtagactgac tttatgggcc tgtcctcgag cggcgtgccg cggtcctctt 3120
cgtagaggaa ctcggaccac tctgagacga aagcacgtgt ccaggccagc acaaaggagg 3180
ctatatggga ggggtagcga tcgttgtcaa ccaaggggtc tactttttcc aaggtgtgta 3240
aacacatgtc cccttcttcc acatccagga aggtgattgg cttgtaagtg tatgccacgt 3300
gacctggggt cccagacggg ggggtataaa agggggcggg tctctgctcg tcctcactgt 3360
cttccggatc gctgtccagg agcgccagct gttgaggtag gtattccctc tcgaaggcgg 3420
gcataacctc cgcactcagg ttgtcagttt ctaggaacga ggaggatttg atattgacag 3480
tgcctgccga gatgcctttc atgagactgt cgtccatttg gtcagaaaag acaatctttt 3540
tgttatcaag tttggtggcg aaggatccga tcgatcgatc catatg 3586
<210> 16
<211> 4138
<212> DNA
<213> 人工序列
<220>
<223> BLY6载体右端
<400> 16
ggatcccccc ttactgtttc caataacgca attgcactta acatggacaa acctttttac 60
agtagcaatg gaaaactatc cttacaagtt acatcaccat taaagatagt cgactcttta 120
aatacattgg ctattggcta tgggcaaggc ttaggactaa acaatagtgc tcttgctgtg 180
caattagcat ctccccttac atttgacagc aacagcaaaa ttaaaataaa tttgggaagc 240
gggccattaa aaattaatgc gaataaactg tcaattaact gcctaagggg tgtatatgta 300
acaactgacg gaacttccat tgaaacaaat ataagctggg caaaaggaat gaggtttgaa 360
ggtaatgcca tggctgtaaa cgttgacagc accaaaggtc tacaatttgg cactaccagc 420
acagaatcag gagtcactaa cgctttccct atccagttaa agattggatc tggtcttagt 480
tttgacagca caggagcact tgtagcttgg gataaggata atgacaagct tacactgtgg 540
acaaccgctg acccatcacc taattgtacc atatatacag acaaggatgc taaacttaca 600
ctttgtctta caaaatgtgg cagtcaaata ctaggcagtg tttcagtact ggctgttaaa 660
gctggaaccc tacagccaat cagtgaaaaa ataggtactg ctttggtttc actaaaattt 720
aataacaacg gtgtattgtt aagcaactcc acattaagta atgaatactg gaactacagg 780
aagggagatg tcacaccagc cgaagcctat actaatgctg tgggttttat gccaaacatc 840
aaggcatatc ctaaaaacac aaactctgcc tcaaaaagcc acattgtagg acaagtgtac 900
cttaatggag atgaaactaa accaatgcat ttaatcatta catttaatga aaccagtgat 960
gaaacatgca catattccat aacgttccaa tggaaatgga acattggaac atacaccagc 1020
gacacccttg caacaagctc ctttaccttt tcttacattg cccaagaata aaaactgcag 1080
acaacaataa agtttaaatg ttttatttaa acagtttcac agaaccctag tattcaacct 1140
gccacctccc tcccaacaca cagagtacac agtcctttct ccccggctgg ccttaaaaag 1200
catcatatca tgggtaacag acatattctt aggtgttata ttccacacgg tttcctgtcg 1260
agccaaacgc tcatcagtga tattaataaa ctccccgggc agctcactta agttcatgtc 1320
gctgtccagc tgctgagcca caggctgctg tccaacttgc ggttgcttaa cgggcggcga 1380
aggagaagtc cacgcctaca tgggggtaga gtcataatcg tgcatcagga tagggcggtg 1440
gtgctgcagc agcgcgcgaa taaactgctg ccgccgccgc tccgtcctgc aggaatacaa 1500
catggcagtg gtctcctcag cgatgattcg caccgcccgc agcataaggc gccttgtcct 1560
ccgggcacag cagcgcaccc tgatctcact taaatcagca cagtaactgc agcacagcac 1620
cacaatattg ttcaaaatcc cacagtgcaa ggcgctgtat ccaaagctca tggcggggac 1680
cacagaaccc acgtggccat cataccacaa gcgcaggtag attaagtggc gacccctcat 1740
aaacacgctg gacataaaca ttacctcttt tggcatgttg taattcacca cctcccggta 1800
ccatataaac ctctgattaa acatggcgcc atccaccacc atcctaaacc agctggccaa 1860
aacctgcccg ccggctatac actgcaggga accgggactg gaacaatgac agtggagagc 1920
ccaggactcg taaccatgga tcatcatgct cgtcatgata tcaatgttgg cacaacacag 1980
gcacacgtgc atacacttcc tcaggattac aagctcctcc cgcgttagaa ccatatccca 2040
gggaacaacc cattcctgaa tcagcgtaaa tcccacactg cagggaagac ctcgcacgta 2100
actcacgttg tgcattgtca aagtgttaca ttcgggcagc agcggatgat cctccagtat 2160
ggtagcgcgg gtttctgtct caaaaggagg tagacgatcc ctactgtacg gagtgcgccg 2220
agacaaccga gatcgtgttg gtcgtagtgt catgccaaat ggaacgccgg acgtagtcat 2280
tctcgtactt tgagtggcaa aaccttgctc tcgaacagca cacgtctcgt cgcctcctgt 2340
cccttctctt cgccttttca gtgtgatagt tgtaatacag ccattcacga agctcagtca 2400
gaagatcttc agcgtctgtt gtcaaaaaca atccatccaa tctgattgct ttcaaaacat 2460
cacaaacagt cgaataagcc aaacccatcc aggcaatgca attattttgg ttatccacaa 2520
tgggaggggg cggaagacat ggaagaggca taattaattt tttaatccaa tcgatcacgc 2580
agcacttcaa aatgaagatc gcgaaggtga cacctttcac ccccactgtg ttgatgaaaa 2640
ataacagcca agtcaaaatt gatgcggttt tcaaggtgct cgactgtagc atcaagcaga 2700
gcttccacac gcacgtccac aaataacaga atagcaaaag cgggaggagg aagtaaatcc 2760
tcaatcatca tagtacagtc catcaccatc cctaaataat tttcatcctt ccagccttgg 2820
actatatttt taaactgctc ttgtaaatcc aaaccacaca tgtggaaaag ttcccaaaga 2880
gctccctcaa ctaccattct taagcacacc ttcatagtga caaaatatct tgttcctctg 2940
tcacctgcag caaattacaa agtccaatat taggatctat gcccagagat ctaagctcat 3000
ccctcaattc caactgtaaa aaggcttcca gatctgccct aacttgttca gccagtgggc 3060
tccctggaat aagcgtggga gaagccaaac tgcaaaacag acgcatgccg ccataattac 3120
caccagaaaa cactacgtta cagtatgcat gctgattcat tccagtaatt tcatccagtg 3180
tattggatac aaaaaaaggc aagcactctc tcactaattg tattatggag acattatcac 3240
acaggtaaca atttaaaggt tgtggaacaa taatgcagta agtaaccacg gtgcgctcca 3300
acatggttag taatttttag ttctgaaaaa caaaacatac aaaaaattat atcatactca 3360
tttggcgaac tggtggaaaa atgaccctat ctagcacaag gcaagccact ggatcaccaa 3420
tgcgcccctc ataaaacctg tcatcatgat taaaaagcaa caccgaaagc tcttccctat 3480
gtcctgcatg aatgattcta gctgaggaat ataagccagc gcaattagta tctgttaaag 3540
aaaaaaaacg gccaacatag cctctaggaa ttagcacact taatcttaaa gacattactg 3600
ccatccccct tggatttaag gtaaaattta caggagcata gaaaatatac tgatttccct 3660
cctgcacagg cagcatagca ccaggtccct ctaaaaacac acacaaagct tctgcagcca 3720
tagcttaccg cgcaaaccag gcacagcagt gagctaaaag gacaaagctc taactcacta 3780
gccaacctgg cgcacaatat atagttagtc cttacactga cgtaaccgac caaagtctaa 3840
aaaccccgcc aaaaatacac acacgcccaa aaaacgcccc gtgagtcaaa aaacagtttc 3900
acttcctcgt tacacccaaa acgtcgtcac ttccggattc ccacggttcg tcacttccgg 3960
agctccttgc ttaattaacc ccgcccaaaa cgtcatcgtc cgcgtcacgc cgccccgccc 4020
cgcgaccgtt gaccccgggc caatcaccgc acatcccgca aaattcaaac tcgtctaatt 4080
tgcatattgg cacactgccc atataaggta tattattgat gatgatttaa atcatatg 4138
<210> 17
<211> 4563
<212> DNA
<213> 人工序列
<220>
<223> BLY6载体中间片段
<400> 17
ggatccatac agggcattgg aaagcagttt ggcaatggag cgcatggttt ggtttttttc 60
tttgtctgcg cgctctttgg cggctatgtt gagttggaca tattcgcggg ccagacattt 120
ccattgtgga aatatggtag ttaattcatc tgggacgatt ctgactttcc agcctctgtt 180
atgcagggta atcagatcca cactggttgc cacttctcct ctaagtggtt cattagtcca 240
gcatagtcgc cccccttttc gagaacagaa agggggtagg ggatctagca tgagttcgtc 300
tggggggtct gcatctatgg tgaaaatccc aggaaggaga tcttcgtcaa aatagctgat 360
ggtggcgggg tcatccagag acatttgcca ttctcgagca gccagagcgc gctcgtaggg 420
gttaagggga gtcccccatg gcatgggatg ggtgagtgca gaagcataca tgccacagat 480
gtcatagaca tagagcggct cttccagaat ccctatgtaa gtgggataac atcgcccccc 540
tctgatgctg gctcgcacat aatcatagag ttcatgtgag ggcgctagaa gacccgagcc 600
caggttggtg cggttgggtt tttctgctct gtagaggatc tggcgaaaga tggcatggga 660
gtttgatgag atggtgggtc tttggaagat gttgaaatgg gcatgaggca gtcccacaga 720
gtcccttatg aagtgagcat aggagtcttg cagtttggcc accagctcgg cggtgaccag 780
cacatccaaa gcacagtagt cgagggtctc tttgatgatg tcatagttag gttccccttt 840
cttttcccac agctcgcggt tgagaaggta ttcttcgcga tccttccagt actcttcgag 900
ggggaacccg tccttgtctg aacggtaaga acccagcatg taaaattgat tgacagcttt 960
gtaggcacaa caccccttct ccacggggag tgagtatgct tgcgcggctt tgcgcagaga 1020
ggtgtgagta agggcgaaag tgtccctgac catgactttg aggaactgat gcttaaagtc 1080
tatgtcatcg caggccccct gctcccacag ttggaagtcc actcgctttt tgtaggcggg 1140
attgggcaaa gcgaaagtaa catcgttgaa taggatcttt ccagccctgg gcatgaagtt 1200
gcgagtaatg cgaaaaggct gaggcacttc tgccctgttg ttgataactt gggcagccaa 1260
gacgatctcg tcaaagccgt tgatgttgtg acccacaatg taaagttcta cgaagcgtgg 1320
gcgtcccttg atgtggggca gttttttaag ctcttcgtag gtcaagtcgt cagggtcagc 1380
gattccatat tgctccaaag cccagtcagg caggtgagga ttagcatgaa ggaaagaggt 1440
ccaaagatcc acggccagag ctgtttgtaa gcggtctctg tactgacgga aatgtcggcc 1500
taccgccatt ttttcaggag taacacagta aaaggtgcgc gggtcctttt cccagcgatc 1560
ccattgaagt tgcaaggcta ggtcgtgggc gaggttgacg agctgttcgt cccccgaaag 1620
tttcatgacc agcatgaaag ggacaagctg cttgccaaag gaccccatcc aggtgtaggt 1680
ttccacatcg taggtgagga agagcctttc tgtgcgagga tgagaaccga tcgggaagaa 1740
ctggatttcc tgccaccagt tggaggaatg gctgttgata tgatggaagt agaactccct 1800
acggcgcgcc gagcattcgt gcttgtgctt gtacagacgg ccacagtact cgcagcgctg 1860
cacgggatgc acctgatgaa tgagctgtac ctggcttcct ttgacaagaa atttcagtgg 1920
gaagttgagg cgtggcgtct gcatctcgtg ttaacccccg taattggccc gccgccctgg 1980
tgtaccagga aactcctgct cccactacag tactacttcc tcgtgacgcc caggccgaag 2040
ttcagatgac taactcaggt gtacagctgg cgggtggtgc caccctgtgt cgtcaccggc 2100
caagaccggg tataaagggc ctggtgatca gaggccgagg tattcagctc aacgacgagt 2160
cggtgaactc ttcgcttggt ctgcgaccag acggcatctt ccaaatagct ggttgtggga 2220
gatcttcctt cactcctcgt caggctgtcc tgactttgga gagttcgtcc tcgcagcccc 2280
gctcgggcgg catcgggact ctccagttcg tggaggagtt tactccctcg gtctacttca 2340
accccttctc cggttctcct gggctttacc cggacgagtt catcccgaac tacgacgcca 2400
tcagtgaagc ggtcgacggc tacgattaat gtctaatggt ggcgcggctg agctagctcg 2460
actgcgacac ctagaccact gccggcgctt tcgctgcttt gctcgggatc tctgcgagtt 2520
catctacttc gagtaccctg acgaacatcc tcagggacct gcccacggag ttcggattac 2580
cattgaaggg gctatcgatt ctcacctgct tcggatcttc accgctcggc cagtgctagt 2640
tgagcgcaac cagggcgaca ccaccatctc cctctgctgc atttgtgaca accccggatt 2700
gcatgaaagc ttttgttgtc ttctttgtac tgagtataat aaaagctgaa attagagact 2760
actccggact ctcttgtcgt ctgaacaaca ccaaccagac ccttcacttc agcgggaacc 2820
agactactct tcactgtaag gcttataact ataagtatct tacttggata tacaaaggaa 2880
caccgtttgc tgtggtaaac aggtgctcca acgacggtgt tctcctcacc ttcctaggca 2940
acttctccaa ctttaccttt tctgttcgca gaaacaagct taccctcctt cagccctact 3000
ttcctgggat ctatacctgc ctcagtggac cttgcaacca cacttttcac ctgattgaaa 3060
actctaccct taccttccca gcgccaatcc ctactaacag ctcggagtcc aactcttcca 3120
ttaccgctga tactaacact cctaaaaccg gaggtgagct ccgcagcctt cccccggctg 3180
cagataaccc ttgggtggta gcgggatttg tagcgctagg aatagttgcg ggtgggctcg 3240
cgttcgtcct ctgctaccta taccttacct gctgctcata tttagtagta ctgtgctgtt 3300
ggtttagaaa atgggggcgc tactaatcac acttgcttta ctttcgcttt tgggtctgag 3360
ctcggctaat agcgagaaac caagctgtct agaaacaaac tctccagact gtgtggttcc 3420
tcatgggctc tcagacccag ctgatgatcc atgcttaact tttgacccag aaaaaaactg 3480
ctcggtgact atgcagccct atgcttacat gtgcacatct gttataaagt gcggatgggg 3540
ctgtaaaccg attgaaatta cccacaaagg caaaacctgg aataatagtt tgtttaacac 3600
atggcagcct ggagacgagc agtggtatac ggccggccac tggtggagat gactgacccc 3660
atggaaaact cctctgccaa cgacctggac atggacggcc gttcatctga gcagcgactg 3720
gtccagatgc gcattcgcca gaagcaggaa cgcgccgcca gagagctcaa ggatgccatt 3780
gaaattcacc tgtgcaagaa gggcatcttt tgcttggtta agcaagcaaa gatttcttat 3840
gaaatcactg acaacgacca ccgcctgtat tatgagctcg gtccacagcg gcagaaattc 3900
acctgcatgg ttggagtcaa ccccatagtc atcactcagc aggctgcaga aattaaaggg 3960
tgcatccact gttcctgtga ttcccaagaa tgcgtccaca ccatagtcaa gaccctctgc 4020
ggccttcgag atcttcttcc aatgaactaa ccccttcccc caacccaata aaacattggt 4080
tttaatcata ataaaaaatc acttacttta aatctgaaac agtgtctccg tccaagtttt 4140
cttgtagcac cacttcactc ccctcttccc agctctggta ctgcaagccc cggtgggctg 4200
caaactttct ccacacctta aaagggatgt caaattcctc ttgtccaaca atcttcattg 4260
tctcttccta gatgtccaca aagcgcgcgc gggtggaaga tgactttgac cctgtctacc 4320
catacgatgc tgagctggca ccgtctgtac ccttcatcgc ccctcccttc gtttcgtcag 4380
acggatttca agaaaaaccc ctgggagttc tgtccctaag actagccaac ccagtcacta 4440
ctaaaaatgg ggaactcaca cttaaactgg gagatggggt gggcatagac tcagatggaa 4500
acctcacagc acagacagtt actaaagcaa catcccccct tactgtttcc aataacgcaa 4560
ttg 4563
<210> 18
<211> 14287
<212> DNA
<213> 人工序列
<220>
<223> BLY6中间质粒3
<400> 18
catcatcaat aatatacctt atatgggcag tgtgccaata tgcaaattag acgagtttga 60
attttgcggg atgtgcggtg attggcccgg ggtcaacggt cgcggggcgg ggcggcgtga 120
cgcggacgat gacgttttgg ggaggaggag ctatgttgca agtaatcgtg ggaaatgcga 180
cgtaaaacga ggtggagttt aaacacggaa gtagacaatt ttcccgcgct gtttgacagg 240
aaatgatgtg tttttgggcg gatgcaagtg aaaattctcc attttcgcgc gaaaactaaa 300
tgaggaagtg aaattctgag taattctgag gttatcacag ggcggagtat ttaccgaggg 360
ccgagtagac tttgacccat tacgtggagg tttcgattac tctatttttc acctaaattt 420
ccgcgtactg tgtcaaagtc cgtgttttta cgcgatcgcg atggcctgtg tttgagcata 480
atgtactgac caggtgtaac gttcatctgg gtggtcgtag aggaatgttc atgccatacc 540
aatgcaattt taatcatgtg aggatcttga tggagccgca agcgttttcc agagtcagct 600
tgactggaat ctttgacatg tgtgtggaag catggaagat cttaagatat gatgatacca 660
aatccagatg ccgcgcatgc gagtgcgggg gcaggcatgc caggttccaa cctgtatgtg 720
tggaggtgac cgaggagctg agaccagatc atttggtgct gacctgcact ggtgcggagt 780
tcggttccag tggtgaagaa actgattaaa gtgagtagtg ggatgttata aaagtgacca 840
taaggtgatg tgagatggac aaatttggta atttttatgt atttttgtct tgcagccatg 900
agtgggagcg cttcctttga agggggcgtc tttagccctt atctgacggg gcgtctgcct 960
cattgggctg gagtgcgtca gaatgtgatg gggtctacag tggatggaag acctgttcag 1020
cctgctaatt cttctactct gacttatgct actatgactt cctcgccttt ggatgcagct 1080
gcagctgctg ccgcttctgc tgccgccaac actgttcggg ggatggcctt ggagatgggg 1140
tattatggaa ctgtagtggc caacaccact accccaaata accccacagc cttgaatgag 1200
gacaagctgc tagttctcat gtcccagctg gagtctttga cccaacgcct gggcgatcta 1260
gctcagcagg tgtcccagct gaaggagcag actcaagctg caattaccac tgcgagggga 1320
aattaaaaaa attcaaagaa tcaataaata aaccgagact ttgttgattt taaagtgtgt 1380
cattctttat ttaatttttc gcgcgcgata tgccctggac caccggtctc tatcattgag 1440
gacacggtgg atcttttcta gaacccgata gaggtgggat tggatgttga ggtacatggg 1500
cataagacca tctttggggt gtagatagct ccactgcaga gcctcatgct ccggggtggt 1560
gttgtatata acccagtcat agcatgggcg ttgggcatga tgttgcacaa tatctttaag 1620
gaggagacta atggccactg ggagaccctt ggtgtaagtg tttacaaatc tattaagctg 1680
ggacgggtgc atccgaggtg agataatgtg cattttggat tggattttta gattggcaat 1740
gtttccccct agatctctcc tgggattcat gttatgcaag accactagaa cagtgtatcc 1800
ggtgcactta gggaatttgt catgaagttt ggaggggaaa gcatgaaaaa atttagacac 1860
acccttgtgt cctcccaagt tctccatgca ctcatccata ataatggcaa tgggcccatg 1920
ggcggcggca cgggcgaaca cgttcctggg atctgacaca tcatagttgt ggtcttgggt 1980
caggtcatca taagccattt taataaactt ggggcggagg gtgccagatt gggggatgaa 2040
tgttccctcg ggccccggaa catagtttcc ttcacatatt tgcatttccc aggcttttag 2100
ttcagagggg gggatcatgt ccacctgtgg agcgatgaag aagacggtct cgggggcggg 2160
ggtgattaag tgggaggaca gcaagttcct aagcagctgt gacttgccac acccagtggg 2220
accgtagatg acccctataa caggttgcag atggtagttt agggaaagac agctgccgtc 2280
ctctcgcagg aggggggcga cctcgttcat catttccctc acatgcatgt tttcccgcac 2340
aagttccgat aggaggcgct ctccacccag ggaaaggagt tcttgaagag atgagaaatt 2400
tttcaagggt tttaagccat cagccatggg cattttggag agggtttgtt gcaagagttc 2460
aaggcggtcc cagagttcgg tgatgtgttc tatggcatct cgatccagca tacttcctcg 2520
tttctggggt tgggacggct gcgggagtat ggaaccaggc gatgggcgtc cagcgctgcc 2580
agtgtccggt ccttccacgg tcgcagcgtc cgagtcaggg tcgtttccgt cacggtgaag 2640
gggtgcgcgc ctggctgggc gcttgcgagg gtgcgcttca ggctcatcct gctcgtggag 2700
aaccgctgcc gttctgcgcc ctgtgcatcg gccaggtagc aattaaccat gagttcgtag 2760
ttgagcgcct ctgccgcgtg gcctttggcg cgcagcttac ctttggaagt cttctgacag 2820
gtgggacagt agagacactt gagagcatag agttttgggg ctagaaagac cgattctggg 2880
gagtatgcat cggccccaca ggaggcgcag acggtttcgc attccaccag ccatgtaaga 2940
tcgggctcgt tggggtcaaa aacaagtttt ccgccatgtt ttttgatgcg tttcttacct 3000
ttgctttcca tgagttcgtg cccccgttgg gtgacaaaga ggctgtccgt gtccccgtag 3060
actgacttta tgggcctgtc ctcgagcggc gtgccgcggt cctcttcgta gaggaactcg 3120
gaccactctg agacgaaagc acgtgtccag gccagcacaa aggaggctat atgggagggg 3180
tagcgatcgt tgtcaaccaa ggggtctact ttttccaagg tgtgtaaaca catgtcccct 3240
tcttccacat ccaggaaggt gattggcttg taagtgtatg ccacgtgacc tggggtccca 3300
gacggggggg tataaaaggg ggcgggtctc tgctcgtcct cactgtcttc cggatcgctg 3360
tccaggagcg ccagctgttg aggtaggtat tccctctcga aggcgggcat aacctccgca 3420
ctcaggttgt cagtttctag gaacgaggag gatttgatat tgacagtgcc tgccgagatg 3480
cctttcatga gactgtcgtc catttggtca gaaaagacaa tctttttgtt atcaagtttg 3540
gtggcgaagg atccatacag ggcattggaa agcagtttgg caatggagcg catggtttgg 3600
tttttttctt tgtctgcgcg ctctttggcg gctatgttga gttggacata ttcgcgggcc 3660
agacatttcc attgtggaaa tatggtagtt aattcatctg ggacgattct gactttccag 3720
cctctgttat gcagggtaat cagatccaca ctggttgcca cttctcctct aagtggttca 3780
ttagtccagc atagtcgccc cccttttcga gaacagaaag ggggtagggg atctagcatg 3840
agttcgtctg gggggtctgc atctatggtg aaaatcccag gaaggagatc ttcgtcaaaa 3900
tagctgatgg tggcggggtc atccagagac atttgccatt ctcgagcagc cagagcgcgc 3960
tcgtaggggt taaggggagt cccccatggc atgggatggg tgagtgcaga agcatacatg 4020
ccacagatgt catagacata gagcggctct tccagaatcc ctatgtaagt gggataacat 4080
cgcccccctc tgatgctggc tcgcacataa tcatagagtt catgtgaggg cgctagaaga 4140
cccgagccca ggttggtgcg gttgggtttt tctgctctgt agaggatctg gcgaaagatg 4200
gcatgggagt ttgatgagat ggtgggtctt tggaagatgt tgaaatgggc atgaggcagt 4260
cccacagagt cccttatgaa gtgagcatag gagtcttgca gtttggccac cagctcggcg 4320
gtgaccagca catccaaagc acagtagtcg agggtctctt tgatgatgtc atagttaggt 4380
tcccctttct tttcccacag ctcgcggttg agaaggtatt cttcgcgatc cttccagtac 4440
tcttcgaggg ggaacccgtc cttgtctgaa cggtaagaac ccagcatgta aaattgattg 4500
acagctttgt aggcacaaca ccccttctcc acggggagtg agtatgcttg cgcggctttg 4560
cgcagagagg tgtgagtaag ggcgaaagtg tccctgacca tgactttgag gaactgatgc 4620
ttaaagtcta tgtcatcgca ggccccctgc tcccacagtt ggaagtccac tcgctttttg 4680
taggcgggat tgggcaaagc gaaagtaaca tcgttgaata ggatctttcc agccctgggc 4740
atgaagttgc gagtaatgcg aaaaggctga ggcacttctg ccctgttgtt gataacttgg 4800
gcagccaaga cgatctcgtc aaagccgttg atgttgtgac ccacaatgta aagttctacg 4860
aagcgtgggc gtcccttgat gtggggcagt tttttaagct cttcgtaggt caagtcgtca 4920
gggtcagcga ttccatattg ctccaaagcc cagtcaggca ggtgaggatt agcatgaagg 4980
aaagaggtcc aaagatccac ggccagagct gtttgtaagc ggtctctgta ctgacggaaa 5040
tgtcggccta ccgccatttt ttcaggagta acacagtaaa aggtgcgcgg gtccttttcc 5100
cagcgatccc attgaagttg caaggctagg tcgtgggcga ggttgacgag ctgttcgtcc 5160
cccgaaagtt tcatgaccag catgaaaggg acaagctgct tgccaaagga ccccatccag 5220
gtgtaggttt ccacatcgta ggtgaggaag agcctttctg tgcgaggatg agaaccgatc 5280
gggaagaact ggatttcctg ccaccagttg gaggaatggc tgttgatatg atggaagtag 5340
aactccctac ggcgcgccga gcattcgtgc ttgtgcttgt acagacggcc acagtactcg 5400
cagcgctgca cgggatgcac ctgatgaatg agctgtacct ggcttccttt gacaagaaat 5460
ttcagtggga agttgaggcg tggcgtctgc atctcgtgtt aacccccgta attggcccgc 5520
cgccctggtg taccaggaaa ctcctgctcc cactacagta ctacttcctc gtgacgccca 5580
ggccgaagtt cagatgacta actcaggtgt acagctggcg ggtggtgcca ccctgtgtcg 5640
tcaccggcca agaccgggta taaagggcct ggtgatcaga ggccgaggta ttcagctcaa 5700
cgacgagtcg gtgaactctt cgcttggtct gcgaccagac ggcatcttcc aaatagctgg 5760
ttgtgggaga tcttccttca ctcctcgtca ggctgtcctg actttggaga gttcgtcctc 5820
gcagccccgc tcgggcggca tcgggactct ccagttcgtg gaggagttta ctccctcggt 5880
ctacttcaac cccttctccg gttctcctgg gctttacccg gacgagttca tcccgaacta 5940
cgacgccatc agtgaagcgg tcgacggcta cgattaatgt ctaatggtgg cgcggctgag 6000
ctagctcgac tgcgacacct agaccactgc cggcgctttc gctgctttgc tcgggatctc 6060
tgcgagttca tctacttcga gtaccctgac gaacatcctc agggacctgc ccacggagtt 6120
cggattacca ttgaaggggc tatcgattct cacctgcttc ggatcttcac cgctcggcca 6180
gtgctagttg agcgcaacca gggcgacacc accatctccc tctgctgcat ttgtgacaac 6240
cccggattgc atgaaagctt ttgttgtctt ctttgtactg agtataataa aagctgaaat 6300
tagagactac tccggactct cttgtcgtct gaacaacacc aaccagaccc ttcacttcag 6360
cgggaaccag actactcttc actgtaaggc ttataactat aagtatctta cttggatata 6420
caaaggaaca ccgtttgctg tggtaaacag gtgctccaac gacggtgttc tcctcacctt 6480
cctaggcaac ttctccaact ttaccttttc tgttcgcaga aacaagctta ccctccttca 6540
gccctacttt cctgggatct atacctgcct cagtggacct tgcaaccaca cttttcacct 6600
gattgaaaac tctaccctta ccttcccagc gccaatccct actaacagct cggagtccaa 6660
ctcttccatt accgctgata ctaacactcc taaaaccgga ggtgagctcc gcagccttcc 6720
cccggctgca gataaccctt gggtggtagc gggatttgta gcgctaggaa tagttgcggg 6780
tgggctcgcg ttcgtcctct gctacctata ccttacctgc tgctcatatt tagtagtact 6840
gtgctgttgg tttagaaaat gggggcgcta ctaatcacac ttgctttact ttcgcttttg 6900
ggtctgagct cggctaatag cgagaaacca agctgtctag aaacaaactc tccagactgt 6960
gtggttcctc atgggctctc agacccagct gatgatccat gcttaacttt tgacccagaa 7020
aaaaactgct cggtgactat gcagccctat gcttacatgt gcacatctgt tataaagtgc 7080
ggatggggct gtaaaccgat tgaaattacc cacaaaggca aaacctggaa taatagtttg 7140
tttaacacat ggcagcctgg agacgagcag tggtatacgg ccggccactg gtggagatga 7200
ctgaccccat ggaaaactcc tctgccaacg acctggacat ggacggccgt tcatctgagc 7260
agcgactggt ccagatgcgc attcgccaga agcaggaacg cgccgccaga gagctcaagg 7320
atgccattga aattcacctg tgcaagaagg gcatcttttg cttggttaag caagcaaaga 7380
tttcttatga aatcactgac aacgaccacc gcctgtatta tgagctcggt ccacagcggc 7440
agaaattcac ctgcatggtt ggagtcaacc ccatagtcat cactcagcag gctgcagaaa 7500
ttaaagggtg catccactgt tcctgtgatt cccaagaatg cgtccacacc atagtcaaga 7560
ccctctgcgg ccttcgagat cttcttccaa tgaactaacc ccttccccca acccaataaa 7620
acattggttt taatcataat aaaaaatcac ttactttaaa tctgaaacag tgtctccgtc 7680
caagttttct tgtagcacca cttcactccc ctcttcccag ctctggtact gcaagccccg 7740
gtgggctgca aactttctcc acaccttaaa agggatgtca aattcctctt gtccaacaat 7800
cttcattgtc tcttcctaga tgtccacaaa gcgcgcgcgg gtggaagatg actttgaccc 7860
tgtctaccca tacgatgctg agctggcacc gtctgtaccc ttcatcgccc ctcccttcgt 7920
ttcgtcagac ggatttcaag aaaaacccct gggagttctg tccctaagac tagccaaccc 7980
agtcactact aaaaatgggg aactcacact taaactggga gatggggtgg gcatagactc 8040
agatggaaac ctcacagcac agacagttac taaagcaaca tcccccctta ctgtttccaa 8100
taacgcaatt gcacttaaca tggacaaacc tttttacagt agcaatggaa aactatcctt 8160
acaagttaca tcaccattaa agatagtcga ctctttaaat acattggcta ttggctatgg 8220
gcaaggctta ggactaaaca atagtgctct tgctgtgcaa ttagcatctc cccttacatt 8280
tgacagcaac agcaaaatta aaataaattt gggaagcggg ccattaaaaa ttaatgcgaa 8340
taaactgtca attaactgcc taaggggtgt atatgtaaca actgacggaa cttccattga 8400
aacaaatata agctgggcaa aaggaatgag gtttgaaggt aatgccatgg ctgtaaacgt 8460
tgacagcacc aaaggtctac aatttggcac taccagcaca gaatcaggag tcactaacgc 8520
tttccctatc cagttaaaga ttggatctgg tcttagtttt gacagcacag gagcacttgt 8580
agcttgggat aaggataatg acaagcttac actgtggaca accgctgacc catcacctaa 8640
ttgtaccata tatacagaca aggatgctaa acttacactt tgtcttacaa aatgtggcag 8700
tcaaatacta ggcagtgttt cagtactggc tgttaaagct ggaaccctac agccaatcag 8760
tgaaaaaata ggtactgctt tggtttcact aaaatttaat aacaacggtg tattgttaag 8820
caactccaca ttaagtaatg aatactggaa ctacaggaag ggagatgtca caccagccga 8880
agcctatact aatgctgtgg gttttatgcc aaacatcaag gcatatccta aaaacacaaa 8940
ctctgcctca aaaagccaca ttgtaggaca agtgtacctt aatggagatg aaactaaacc 9000
aatgcattta atcattacat ttaatgaaac cagtgatgaa acatgcacat attccataac 9060
gttccaatgg aaatggaaca ttggaacata caccagcgac acccttgcaa caagctcctt 9120
taccttttct tacattgccc aagaataaaa actgcagaca acaataaagt ttaaatgttt 9180
tatttaaaca gtttcacaga accctagtat tcaacctgcc acctccctcc caacacacag 9240
agtacacagt cctttctccc cggctggcct taaaaagcat catatcatgg gtaacagaca 9300
tattcttagg tgttatattc cacacggttt cctgtcgagc caaacgctca tcagtgatat 9360
taataaactc cccgggcagc tcacttaagt tcatgtcgct gtccagctgc tgagccacag 9420
gctgctgtcc aacttgcggt tgcttaacgg gcggcgaagg agaagtccac gcctacatgg 9480
gggtagagtc ataatcgtgc atcaggatag ggcggtggtg ctgcagcagc gcgcgaataa 9540
actgctgccg ccgccgctcc gtcctgcagg aatacaacat ggcagtggtc tcctcagcga 9600
tgattcgcac cgcccgcagc ataaggcgcc ttgtcctccg ggcacagcag cgcaccctga 9660
tctcacttaa atcagcacag taactgcagc acagcaccac aatattgttc aaaatcccac 9720
agtgcaaggc gctgtatcca aagctcatgg cggggaccac agaacccacg tggccatcat 9780
accacaagcg caggtagatt aagtggcgac ccctcataaa cacgctggac ataaacatta 9840
cctcttttgg catgttgtaa ttcaccacct cccggtacca tataaacctc tgattaaaca 9900
tggcgccatc caccaccatc ctaaaccagc tggccaaaac ctgcccgccg gctatacact 9960
gcagggaacc gggactggaa caatgacagt ggagagccca ggactcgtaa ccatggatca 10020
tcatgctcgt catgatatca atgttggcac aacacaggca cacgtgcata cacttcctca 10080
ggattacaag ctcctcccgc gttagaacca tatcccaggg aacaacccat tcctgaatca 10140
gcgtaaatcc cacactgcag ggaagacctc gcacgtaact cacgttgtgc attgtcaaag 10200
tgttacattc gggcagcagc ggatgatcct ccagtatggt agcgcgggtt tctgtctcaa 10260
aaggaggtag acgatcccta ctgtacggag tgcgccgaga caaccgagat cgtgttggtc 10320
gtagtgtcat gccaaatgga acgccggacg tagtcattct cgtactttga gtggcaaaac 10380
cttgctctcg aacagcacac gtctcgtcgc ctcctgtccc ttctcttcgc cttttcagtg 10440
tgatagttgt aatacagcca ttcacgaagc tcagtcagaa gatcttcagc gtctgttgtc 10500
aaaaacaatc catccaatct gattgctttc aaaacatcac aaacagtcga ataagccaaa 10560
cccatccagg caatgcaatt attttggtta tccacaatgg gagggggcgg aagacatgga 10620
agaggcataa ttaatttttt aatccaatcg atcacgcagc acttcaaaat gaagatcgcg 10680
aaggtgacac ctttcacccc cactgtgttg atgaaaaata acagccaagt caaaattgat 10740
gcggttttca aggtgctcga ctgtagcatc aagcagagct tccacacgca cgtccacaaa 10800
taacagaata gcaaaagcgg gaggaggaag taaatcctca atcatcatag tacagtccat 10860
caccatccct aaataatttt catccttcca gccttggact atatttttaa actgctcttg 10920
taaatccaaa ccacacatgt ggaaaagttc ccaaagagct ccctcaacta ccattcttaa 10980
gcacaccttc atagtgacaa aatatcttgt tcctctgtca cctgcagcaa attacaaagt 11040
ccaatattag gatctatgcc cagagatcta agctcatccc tcaattccaa ctgtaaaaag 11100
gcttccagat ctgccctaac ttgttcagcc agtgggctcc ctggaataag cgtgggagaa 11160
gccaaactgc aaaacagacg catgccgcca taattaccac cagaaaacac tacgttacag 11220
tatgcatgct gattcattcc agtaatttca tccagtgtat tggatacaaa aaaaggcaag 11280
cactctctca ctaattgtat tatggagaca ttatcacaca ggtaacaatt taaaggttgt 11340
ggaacaataa tgcagtaagt aaccacggtg cgctccaaca tggttagtaa tttttagttc 11400
tgaaaaacaa aacatacaaa aaattatatc atactcattt ggcgaactgg tggaaaaatg 11460
accctatcta gcacaaggca agccactgga tcaccaatgc gcccctcata aaacctgtca 11520
tcatgattaa aaagcaacac cgaaagctct tccctatgtc ctgcatgaat gattctagct 11580
gaggaatata agccagcgca attagtatct gttaaagaaa aaaaacggcc aacatagcct 11640
ctaggaatta gcacacttaa tcttaaagac attactgcca tcccccttgg atttaaggta 11700
aaatttacag gagcatagaa aatatactga tttccctcct gcacaggcag catagcacca 11760
ggtccctcta aaaacacaca caaagcttct gcagccatag cttaccgcgc aaaccaggca 11820
cagcagtgag ctaaaaggac aaagctctaa ctcactagcc aacctggcgc acaatatata 11880
gttagtcctt acactgacgt aaccgaccaa agtctaaaaa ccccgccaaa aatacacaca 11940
cgcccaaaaa acgccccgtg agtcaaaaaa cagtttcact tcctcgttac acccaaaacg 12000
tcgtcacttc cggattccca cggttcgtca cttccggagc tccttgctta attaaccccg 12060
cccaaaacgt catcgtccgc gtcacgccgc cccgccccgc gaccgttgac cccgggccaa 12120
tcaccgcaca tcccgcaaaa ttcaaactcg tctaatttgc atattggcac actgcccata 12180
taaggtatat tattgatgat gatttaaatc atatgcggtg tgaaataccg cacagatgcg 12240
taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct 12300
cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 12360
cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 12420
accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 12480
acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 12540
cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 12600
acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 12660
atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 12720
agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 12780
acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 12840
gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 12900
gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 12960
gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 13020
gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 13080
acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 13140
tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 13200
ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 13260
catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 13320
ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 13380
caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 13440
ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 13500
tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg 13560
cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 13620
aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 13680
tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 13740
gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 13800
cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa 13860
aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 13920
tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 13980
tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 14040
gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 14100
atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 14160
taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta 14220
tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattga 14280
tttaaat 14287
<210> 19
<211> 638
<212> DNA
<213> 人工序列
<220>
<223> Ad5 pIX启动子插入
<400> 19
cgcgatcgcg gtactgaaat gtgtgggcgt ggcttaaggg tgggaaagaa tatataaggt 60
gggggtctta tgtagttttg tatctgtttt gcagcagccg ccgccgccat gagtgggagc 120
gcttcctttg aagggggcgt ctttagccct tatctgacgg ggcgtctgcc tcattgggct 180
ggagtgcgtc agaatgtgat ggggtctaca gtggatggaa gacctgttca gcctgctaat 240
tcttctactc tgacttatgc tactatgact tcctcgcctt tggatgcagc tgcagctgct 300
gccgcttctg ctgccgccaa cactgttcgg gggatggcct tggagatggg gtattatgga 360
actgtagtgg ccaacaccac taccccaaat aaccccacag ccttgaatga ggacaagctg 420
ctagttctca tgtcccagct ggagtctttg acccaacgcc tgggcgatct agctcagcag 480
gtgtcccagc tgaaggagca gactcaagct gcaattacca ctgcgagggg aaattaaaaa 540
aattcaaaga atcaataaat aaaccgagac tttgttgatt ttaaagtgtg tcattcttta 600
tttaattttt cgcgcgcgat atgccctgga ccaccggt 638
<210> 20
<211> 35711
<212> DNA
<213> 人工序列
<220>
<223> pBLY6.FLuc
<400> 20
catcatcaat aatatacctt atatgggcag tgtgccaata tgcaaattag acgagtttga 60
attttgcggg atgtgcggtg attggcccgg ggtcaacggt cgcggggcgg ggcggcgtga 120
cgcggacgat gacgttttgg ggaggaggag ctatgttgca agtaatcgtg ggaaatgcga 180
cgtaaaacga ggtggagttt aaacacggaa gtagacaatt ttcccgcgct gtttgacagg 240
aaatgatgtg tttttgggcg gatgcaagtg aaaattctcc attttcgcgc gaaaactaaa 300
tgaggaagtg aaattctgag taattctgag gttatcacag ggcggagtat ttaccgaggg 360
ccgagtagac tttgacccat tacgtggagg tttcgattac tctatttttc acctaaattt 420
ccgcgtactg tgtcaaagtc cgtgttttta cggcgatcgc tcaatattgg ccattagcca 480
tattattcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt 540
atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg ccatgttgac 600
attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 660
atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 720
acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 780
tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 840
tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 900
attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 960
tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 1020
ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 1080
accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 1140
gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga 1200
tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca 1260
gcctccgcgg ccgggaacgg tgcattggaa gcttggcatt ccggtactgt tggtaaagcc 1320
accatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat 1380
ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca 1440
attgctttta cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg 1500
tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc 1560
gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga 1620
gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc 1680
atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg 1740
caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg attctaaaac ggattaccag 1800
ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac 1860
gattttgtgc cagagtcctt cgatagggac aagacaattg cactgatcat gaactcctct 1920
ggatctactg gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg cgtgagattc 1980
tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt 2040
gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga 2100
tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttctgaggag ccttcaggat 2160
tacaagattc aaagtgcgct gctggtgcca accctattct ccttcttcgc caaaagcact 2220
ctgattgaca aatacgattt atctaattta cacgaaattg cttctggtgg cgctcccctc 2280
tctaaggaag tcggggaagc ggttgccaag aggttccatc tgccaggtat caggcaagga 2340
tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg 2400
ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg 2460
aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc 2520
ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat 2580
tctggagaca tagcttactg ggacgaagac gaacacttct tcatcgttga ccgcctgaag 2640
tctctgatta agtacaaagg ctatcaggtg gctcccgctg aattggaatc catcttgctc 2700
caacacccca acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa 2760
cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg 2820
gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg 2880
gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc 2940
ataaaggcca agaagggcgg aaagatcgcc gtgtaattct agacgagatc cgaacttgtt 3000
tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 3060
atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 3120
ctgcgatcgc gatggcctgt gtttgagcat aatgtactga ccaggtgtaa cgttcatctg 3180
ggtggtcgta gaggaatgtt catgccatac caatgcaatt ttaatcatgt gaggatcttg 3240
atggagccgc aagcgttttc cagagtcagc ttgactggaa tctttgacat gtgtgtggaa 3300
gcatggaaga tcttaagata tgatgatacc aaatccagat gccgcgcatg cgagtgcggg 3360
ggcaggcatg ccaggttcca acctgtatgt gtggaggtga ccgaggagct gagaccagat 3420
catttggtgc tgacctgcac tggtgcggag ttcggttcca gtggtgaaga aactgattaa 3480
agtgagtagt gggatgttat aaaagtgacc ataaggtgat gtgagatgga caaatttggt 3540
aatttttatg tatttttgtc ttgcagccat gagtgggagc gcttcctttg aagggggcgt 3600
ctttagccct tatctgacgg ggcgtctgcc tcattgggct ggagtgcgtc agaatgtgat 3660
ggggtctaca gtggatggaa gacctgttca gcctgctaat tcttctactc tgacttatgc 3720
tactatgact tcctcgcctt tggatgcagc tgcagctgct gccgcttctg ctgccgccaa 3780
cactgttcgg gggatggcct tggagatggg gtattatgga actgtagtgg ccaacaccac 3840
taccccaaat aaccccacag ccttgaatga ggacaagctg ctagttctca tgtcccagct 3900
ggagtctttg acccaacgcc tgggcgatct agctcagcag gtgtcccagc tgaaggagca 3960
gactcaagct gcaattacca ctgcgagggg aaattaaaaa aattcaaaga atcaataaat 4020
aaaccgagac tttgttgatt ttaaagtgtg tcattcttta tttaattttt cgcgcgcgat 4080
atgccctgga ccaccggtct ctatcattga ggacacggtg gatcttttct agaacccgat 4140
agaggtggga ttggatgttg aggtacatgg gcataagacc atctttgggg tgtagatagc 4200
tccactgcag agcctcatgc tccggggtgg tgttgtatat aacccagtca tagcatgggc 4260
gttgggcatg atgttgcaca atatctttaa ggaggagact aatggccact gggagaccct 4320
tggtgtaagt gtttacaaat ctattaagct gggacgggtg catccgaggt gagataatgt 4380
gcattttgga ttggattttt agattggcaa tgtttccccc tagatctctc ctgggattca 4440
tgttatgcaa gaccactaga acagtgtatc cggtgcactt agggaatttg tcatgaagtt 4500
tggaggggaa agcatgaaaa aatttagaca cacccttgtg tcctcccaag ttctccatgc 4560
actcatccat aataatggca atgggcccat gggcggcggc acgggcgaac acgttcctgg 4620
gatctgacac atcatagttg tggtcttggg tcaggtcatc ataagccatt ttaataaact 4680
tggggcggag ggtgccagat tgggggatga atgttccctc gggccccgga acatagtttc 4740
cttcacatat ttgcatttcc caggctttta gttcagaggg ggggatcatg tccacctgtg 4800
gagcgatgaa gaagacggtc tcgggggcgg gggtgattaa gtgggaggac agcaagttcc 4860
taagcagctg tgacttgcca cacccagtgg gaccgtagat gacccctata acaggttgca 4920
gatggtagtt tagggaaaga cagctgccgt cctctcgcag gaggggggcg acctcgttca 4980
tcatttccct cacatgcatg ttttcccgca caagttccga taggaggcgc tctccaccca 5040
gggaaaggag ttcttgaaga gatgagaaat ttttcaaggg ttttaagcca tcagccatgg 5100
gcattttgga gagggtttgt tgcaagagtt caaggcggtc ccagagttcg gtgatgtgtt 5160
ctatggcatc tcgatccagc atacttcctc gtttctgggg ttgggacggc tgcgggagta 5220
tggaaccagg cgatgggcgt ccagcgctgc cagtgtccgg tccttccacg gtcgcagcgt 5280
ccgagtcagg gtcgtttccg tcacggtgaa ggggtgcgcg cctggctggg cgcttgcgag 5340
ggtgcgcttc aggctcatcc tgctcgtgga gaaccgctgc cgttctgcgc cctgtgcatc 5400
ggccaggtag caattaacca tgagttcgta gttgagcgcc tctgccgcgt ggcctttggc 5460
gcgcagctta cctttggaag tcttctgaca ggtgggacag tagagacact tgagagcata 5520
gagttttggg gctagaaaga ccgattctgg ggagtatgca tcggccccac aggaggcgca 5580
gacggtttcg cattccacca gccatgtaag atcgggctcg ttggggtcaa aaacaagttt 5640
tccgccatgt tttttgatgc gtttcttacc tttgctttcc atgagttcgt gcccccgttg 5700
ggtgacaaag aggctgtccg tgtccccgta gactgacttt atgggcctgt cctcgagcgg 5760
cgtgccgcgg tcctcttcgt agaggaactc ggaccactct gagacgaaag cacgtgtcca 5820
ggccagcaca aaggaggcta tatgggaggg gtagcgatcg ttgtcaacca aggggtctac 5880
tttttccaag gtgtgtaaac acatgtcccc ttcttccaca tccaggaagg tgattggctt 5940
gtaagtgtat gccacgtgac ctggggtccc agacgggggg gtataaaagg gggcgggtct 6000
ctgctcgtcc tcactgtctt ccggatcgct gtccaggagc gccagctgtt gaggtaggta 6060
ttccctctcg aaggcgggca taacctccgc actcaggttg tcagtttcta ggaacgagga 6120
ggatttgata ttgacagtgc ctgccgagat gcctttcatg agactgtcgt ccatttggtc 6180
agaaaagaca atctttttgt tatcaagttt ggtggcgaag gatccataca gggcattgga 6240
aagcagtttg gcaatggagc gcatggtttg gtttttttct ttgtctgcgc gctctttggc 6300
ggctatgttg agttggacat attcgcgggc cagacatttc cattgtggaa atatggtagt 6360
taattcatct gggacgattc tgactttcca gcctctgtta tgcagggtaa tcagatccac 6420
actggttgcc acttctcctc taagtggttc attagtccag catagtcgcc ccccttttcg 6480
agaacagaaa gggggtaggg gatctagcat gagttcgtct ggggggtctg catctatggt 6540
gaaaatccca ggaaggagat cttcgtcaaa atagctgatg gtggcggggt catccagaga 6600
catttgccat tctcgagcag ccagagcgcg ctcgtagggg ttaaggggag tcccccatgg 6660
catgggatgg gtgagtgcag aagcatacat gccacagatg tcatagacat agagcggctc 6720
ttccagaatc cctatgtaag tgggataaca tcgcccccct ctgatgctgg ctcgcacata 6780
atcatagagt tcatgtgagg gcgctagaag acccgagccc aggttggtgc ggttgggttt 6840
ttctgctctg tagaggatct ggcgaaagat ggcatgggag tttgatgaga tggtgggtct 6900
ttggaagatg ttgaaatggg catgaggcag tcccacagag tcccttatga agtgagcata 6960
ggagtcttgc agtttggcca ccagctcggc ggtgaccagc acatccaaag cacagtagtc 7020
gagggtctct ttgatgatgt catagttagg ttcccctttc ttttcccaca gctcgcggtt 7080
gagaaggtat tcttcgcgat ccttccagta ctcttcgagg gggaacccgt ccttgtctga 7140
acggtaagaa cccagcatgt aaaattgatt gacagctttg taggcacaac accccttctc 7200
cacggggagt gagtatgctt gcgcggcttt gcgcagagag gtgtgagtaa gggcgaaagt 7260
gtccctgacc atgactttga ggaactgatg cttaaagtct atgtcatcgc aggccccctg 7320
ctcccacagt tggaagtcca ctcgcttttt gtaggcggga ttgggcaaag cgaaagtaac 7380
atcgttgaat aggatctttc cagccctggg catgaagttg cgagtaatgc gaaaaggctg 7440
aggcacttct gccctgttgt tgataacttg ggcagccaag acgatctcgt caaagccgtt 7500
gatgttgtga cccacaatgt aaagttctac gaagcgtggg cgtcccttga tgtggggcag 7560
ttttttaagc tcttcgtagg tcaagtcgtc agggtcagcg attccatatt gctccaaagc 7620
ccagtcaggc aggtgaggat tagcatgaag gaaagaggtc caaagatcca cggccagagc 7680
tgtttgtaag cggtctctgt actgacggaa atgtcggcct accgccattt tttcaggagt 7740
aacacagtaa aaggtgcgcg ggtccttttc ccagcgatcc cattgaagtt gcaaggctag 7800
gtcgtgggcg aggttgacga gctgttcgtc ccccgaaagt ttcatgacca gcatgaaagg 7860
gacaagctgc ttgccaaagg accccatcca ggtgtaggtt tccacatcgt aggtgaggaa 7920
gagcctttct gtgcgaggat gagaaccgat cgggaagaac tggatttcct gccaccagtt 7980
ggaggaatgg ctgttgatat gatggaagta gaactcccta cggcgcgccg agcattcgtg 8040
cttgtgcttg tacagacggc cacagtactc gcagcgctgc acgggatgca cctgatgaat 8100
gagctgtacc tggcttcctt tgacaagaaa tttcagtggg aagttgaggc gtggcgtctg 8160
catctcgtgt tgtattacgt cctggctatt ggtctggcca tcttctgtct cgatggtggt 8220
catgctgacg agcccgcgcg ggaggcaggt ccagacctcc gcgcggacgg gtctgagagc 8280
gaggacgaga gcgcgcaggc cggaactgtc cagggtcctg agacgctgcg gagtcaggtc 8340
agtagggaga gtacataggt ttacttgcat aagtttttcc agggcatgtg ggaggtcaag 8400
atgatatttg atttctactg gcgagttggt ggagacatcg atggcttgca gggtcccgtg 8460
cccctggggt gctaccaccg tccctttttt tttcttgatc gggggcggtg ttgcttcttg 8520
catggtaagg tcgtcttcta gaagcggcgg cgaggtcgcg cgccgggtgg cagtggcggt 8580
tctggacctg gaggtagagg cggtagaggt acgtcggcgc cgcgcgcggg taggttctgg 8640
tactgcgccc tgagaagact tgcgtgagcg acgacgcggc ggttgacgtc ctggatctga 8700
cgcctctggg tgaatgctac cggacccgtg agcttgaacc tgaaagagag ttcaacagaa 8760
tcaatttcgg tatcgttgac ggctgcctgc cgcaggattt cttgtacgtc gcccgagttg 8820
tcttggtagg cgatctcggc catgaactgc tcgatctctt cttcttggag atctccgcgg 8880
cccgctcgtt ctacggtggc agcaaggtcg ttggagatgc gccccatgag ctgtgagaat 8940
gcattcatgc ccgcctcgtt ccagacgcga ctgtagacca cggctccctc gggatctctg 9000
gcgcgcatga ccacttgggc gaggtttagt tccacgtgtc tggtgaagac cgcatagttg 9060
cagagacgct ggaagaggta gttgagcgtg gtggcgatgt gctcggtgac aaagaaatac 9120
atgatccagc gacgaagcgg catctcgctg atatcgccca gggcttccaa ccgttccatg 9180
gcttcgtaaa agtccacggc gaagttgaaa aactgggagt tgcgagcgga cacggtcaac 9240
tcctcctcca gaagacggat gagctcggcg atggtggcgc gcacttcgcg ctcaaaggct 9300
cccgggatct cttcctcctc ttcttcttcc aactcttcct ccactaacat ctcttctact 9360
tcctcctcag gcggcggggg tggaggaggg ggcgcgcggc gacgccggcg acgcacgggc 9420
agacgatcga tgaagcgttc gatcacttct ccgcggcggc gacgcatggt ctcggtgacg 9480
gcgcgcccgt cctccctggg tcgcagagtg aagacgccgc cgcgcagctc cctgaaatgg 9540
tgactgggag ggtccccgtt tggtagggac agggcactga tgatgcatct tattaattgc 9600
cctgtaggga ctccgcgcaa ggacctgagc gtctcgagat ccacgggatc tgaaaatctc 9660
tgaacgaagg cttcgagcca gtcgcagtcg caaggtaggc tgagcactgt ttcttcgggg 9720
cgggctgctg agctagaggg ttgtacgatg ctgctggtga tgaagttaaa ataggcagtt 9780
ctgagacggc ggatggtggc gaggagcacc aggtctttgg gtccggcttg ctggatgcgc 9840
aggcggtcgg ccattcccca tgcattatct tggcacctgg ccagatcttt atagtagtct 9900
tgcatgagtc gctccacggg cacttcttct tcgcccgctc tgccgtgcat gcgtgtgagt 9960
ccgtaccctc tctgtggttg gacgagcgcc aggtcggcaa cgaccctttc ggctagaatg 10020
gcttgctgca cctgggtgag ggtgttctgg aaatcatcaa agtccacaaa gcggtggtag 10080
gcccccgtgt tgatggtgta ggagcagttg gacatgaccg accagttgac tgtctggtgt 10140
cctggtcgta cgagttccgt gtacctgagc cgcgagtatg cgcgggagtc gaagatgtaa 10200
tcgttgcagg ttcgcaccag gtactggtag ccgatgagga agtgaggcgg cggctggcgg 10260
tagagaggcc atcgttcggt ggcgggcgcg ccgggcgcta ggtcttctag catgagacgg 10320
tggtatccgt agacgtacct ggacatccag gtaataccgg cggcggtggt ggaggcgcgc 10380
ggaaactctc gcacgcggtt ccagatgttg cgcagcggca tgaagtagtt catggtgggc 10440
acggtctggc ccgtgaggcg cgcgcagtca ttgatgctct agatacgggc aaaaacgaaa 10500
gcgttgagcg gttcccttcc gtggcctgga ggaacgcgaa cgggttaggt cgcagcgtac 10560
cctggttcga gactaaagaa agcgagcaac tcgaaccggc agagtcgcgg ctaacgggta 10620
ttggcaatcc cgtctcgacc caagccagca aatccaggat acggatgggg gccccttttg 10680
tttttcaggg catgagtcac cggttaaggt ttacaacggc tgtttcatgc ctttagaagt 10740
ggctcgcgcc cgtagtctgg agaatcaatc gccagggttg cgttgcggcg tgccccggtt 10800
cgagcctgca gcttgagtcg gccggtgacc gcggcaaacg agggcgtggc ggccccgtcg 10860
tttctaagac cttgctagcc gacctctcca gtttacggga acgagccccc ttttattttt 10920
tttgtttttg ccagatgcat cccgtactgc ggcagatgcg cccacagccc ccacagcagc 10980
agcagcaggc tggcctacct tctctacctc agccgctacc tgcaactacc gcggtggccg 11040
ctgtaagcgg ggccggacag caggcggctc ctcaatatga attggacttg gaagagggcg 11100
agggattggc aagattgggg gcgccctcgc ccgagcgcca cccgcgggtg cagatgaaaa 11160
aggacgttcg cgaatcttac gtgcccaagc agaatctgtt cagagacaga agcggcgagg 11220
agcccgagga gatgcgcgcg tcccgtttta acgcgggtcg cgagctgcga caaggactgg 11280
atcgaaaacg ggtgttgagg gatgatgatt ttgaggtgga tgaaatgaca gggatcagcc 11340
ccgctcgcgc tcacgtggct gcagctaatc tggtgacagc ttatgagcag accgtgaagg 11400
aggaaagcaa cttccagaaa tcattcaata accacgtgcg caccctgatc gcacgcgagg 11460
aggtgaccct gggcctgatg cacctgtggg atctgctgga agccatagtg cagaacccca 11520
ctagcaaacc cctgactgct caactgtttc tggtggtgca gcacagcagg gataatgagg 11580
cattcagaga ggcgctgctg aatatcactg aacctgaggg gagatggctg ctggatctgg 11640
tgaatatcct gcagagcatt gtagtgcagg aacgcagctt gcctttgtcc gagaaggtgg 11700
cggcgatcaa ttactctgtg ctgagtctgg gcaaatacta tgccaggaag atctacaaaa 11760
ccccttacgt gcccatagac aaggaagtga aaatagatgg gttttacatg cgcatgaccc 11820
tgaaagtgct aaccctgagc gatgacttgg gagtgtaccg caacgacagg atgcaccgcg 11880
cggtgagcgc cagcaggagg cgcgagctga gcgacaaaga attaatgcac agcttgcaac 11940
gagccctgac gggagccggg acggaggggg agaactactt tgacatgggt gcagacttgc 12000
attggctgcc tagtcgcagg gcattggaag cggcaggcga tgggccctat gtagaggaag 12060
tagtagacga ggacgatgag gagggcgagt acctggaaga ctgatggcgc gacccgtatt 12120
tttgctagat ggaacaggcg ccggaccctg cgatgcgggc ggcgctgcag agccagccgt 12180
ccggcattaa ttcctcggac gattggaccc aggccatgca acgcatcatg gcgctgacga 12240
cccgcaaccc cgaagccttt agacagcagc ctcaggccaa ccgcctttcg gccatcctgg 12300
aggccgtggt gccctctcgc tccaacccca cccacgagaa ggttctggcc atcgtgaatg 12360
ccctggtgga gaacaaggcc atccgctccg atgaagccgg gctggtatac aacgccttgc 12420
tcgagcgcgt ggctcgctac aacagcagca atgtccagac taacctggac aggatggtga 12480
ccgacgtgcg cgaggccgtg tcccagcgcg aacggttcca tcgcgagtct aacctgggtt 12540
ccatggtagc gctgaacgct ttcctcagtt cccagcctgc caatgtgccc cggggacagg 12600
aagactatac caactttatt agcgccctga gactcatggt agccgaggtt cctcagagcg 12660
aggtgtacca gtccggtcca gactactttt tccagacaag caggaacggt atgcagacag 12720
tgaacttaag ccaggctttc aagaacctgc aagggctgtg gggagtccaa gctccagtgg 12780
gcgacagggc gaccgtgtcg agcctgttga ctccaaattc ccgtttgctg ctgctgctgg 12840
tgtccccctt cactgacagc ggcagcataa acagaaactc ctacttgggc tacctgataa 12900
acttgtatcg cgaagctata ggtcaggccc acgtggacga acagacctat caggagatca 12960
ctaatgtgag tcgcgctctg ggccaggacg accctggaaa cctggaagct actctaaact 13020
ttctgctgac caaccgctcg caaaaaatcc ctcctcagta tacattaact gcggaggagg 13080
aacggatctt gagatacgtg cagcagagcg tgggtctgtt cctgatgcaa gagggtgcga 13140
cccctagcgc cgcgcttgat atgacagcgc gcaacatgga gcccagcatg tatgccagca 13200
acagaccatt cattaataaa ttgatggatt acttccatcg cgcggccgct atgaactctg 13260
attacttcac caatgctatt ctgaaccccc attggctgcc tccgcctggt ttttatactg 13320
gcgagtatga catgcctgac cccaacgatg ggttcttgtg ggacgatgtg gacagcgtgg 13380
cgttctcgcc taccgctcct cgtacttttt ggaagaagga aggtagtgac agaagaccct 13440
cctccgtgct gtcaggacgt gagggtgctg ccgcggcggt ccccgatgct gcaagcccct 13500
ttcccagtct gccattttca ctaaacagcg tgcgcagtag cgagctgggg agaataaccc 13560
gccctcgctt gctgggcgag gacgagtatt tgaatgactc cctactgaga cccgagcggg 13620
aaaagaactt ccctaataat gggattgaaa gcctggtgga taagatgagc agatggaaga 13680
cctatgccca ggagcacaga gatgagccta gaatcttggg tcctacagta ggcacccgca 13740
gacgccagcg ccatgataga cagcggggtc tggtgtggga cgatgaggat tctgcagatg 13800
acagcagcgt gttggacttg ggcgggaggg gaggtgtggg caacccgttc gcacacttgc 13860
gtccccgtat tggacgcatg atgtaaaagt gaaaataaaa aaggaactca ccaaggccat 13920
ggcgaccagc gtgcgttcgt tctttctgtt gttgtatcta gtatgatgag gcgcaccgtg 13980
ctaggcggat cggtggcgta tccggagggt cctcctcctt cgtacgaaag cgtgatgcag 14040
caggtggcgg cggcggcgat gcaacccccc ttggaggctc cttacgtgcc cccgcggtac 14100
ctggcaccta ccgaggggag aaacagcatt cgttattcgg aactcacacc cttgtatgac 14160
accacccggt tgtacctggt ggacaacaaa tcggcggaca ttgcctcgtt gaactatcag 14220
aacgaccaca gcaacttctt gacaacggtg gtgcagaaca atgactttac ccccacggag 14280
gccagcaccc agaccatcaa ctttgacgag cgctcccggt ggggcggtca gctgaagacc 14340
atcatgcaca ccaacatgcc caacgtgaac gagttcatgt ttagcaacaa gttcagggct 14400
agggtgatgg tgtccagaac cacacctaaa gaggtgacag tcacaacaga ctatgatggt 14460
agtcaggaca tcttggaata cgagtgggtt gactttgagt taccagaagg caacttctct 14520
gccaccatga ccatagacct gatgaataat gcaattgttg ataattacct aaaagtgggt 14580
agacagaatg gggtactgga gagtgacata ggtgttaagt ttgacactag gaactttagg 14640
cttggttggg acccagtgac agagttggtc atgcctgggg tctacaccaa tgaagctttc 14700
catcctgaca tagtcctact acctggctgc ggagtggact tcactgagag ccgcctcagt 14760
aatctgctag gcattagaaa gaaacagcca ttccaggaag ggttccagat catgtatgag 14820
gatctggagg gtggtaacat ccccgccctg cttgatgtaa atgcatatga gaagagcaag 14880
gaagataata caaccaccac aaatgaagct gtggccgcgg cttcatctac tgaagccaaa 14940
gctgtggtag atgcttccac ttcaacagaa aacaccactg atgaaaaagt caccagggga 15000
gatacatttg ccacccctga acaagagaag gcagctgagg cagagtctga tattatgctt 15060
ctgtccaccg atgaaaacga aactaaaaaa caactggtta ttcgagcggt gaccaaggat 15120
agtaaggaca ggagttataa tgtattgtca gatggaaaga acacagctta ccgtagttgg 15180
tacctggcat acaattatgg cgaccgtgag aaaggggtgc gttcttggac actgcttacc 15240
acctcggatg tcacctgcgg cgtggagcaa gtctattggt cgctaccaga tatgatgcaa 15300
gatccagtca cctttcgctc cacacgccaa gttagcaact acccagtggt gggcgcagag 15360
ctgctcccag tgcattccag aagcttctac aacgagcaag ccgtctactc gcaacagctc 15420
cgccagtaca cctcgctcac gcacgtcttc aaccgcttcc ccgagaatca gatcctcgtc 15480
cgcccgcccg cgccaaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac 15540
gggaccctgc cgctgcgcag cagtatccgg ggagtccagc gcgtgaccgt tactgacgcc 15600
agacgccgca cctgtccata cgtatacaag gccctgggca tagtcgcgcc gcgcgtcctt 15660
tcaagccgca ctttctaaaa aaatgtccat tctcatctcg cccagtaata acaccggttg 15720
gggcctgcgc acacctagca agatgtatgg aggcgctcgc agacgctcca ctcagcaccc 15780
tgtgcgcgtg cgcgggcatt tccgcgctcc ctggggcgcc ctcaagggac gctctcgtac 15840
taggaccacc gttgacgatg tgatcgacca ggtggtcgcc gatgcacgta actatacccc 15900
cgcagccgca cctgcatcca ccgtggatgc ggtcattgac agcgtggtag ccgatgcgcg 15960
cgcctatgct cgcgccaaga gcaggaggcg gcgtattgcc aggcgtcacc gagctactcc 16020
agccatgcga gctgcaagag ctttattgcg gagagccaga cgtgtggggc gaagagccat 16080
gcgtagagcg gccagacgcg cggcttcagg tgccagcgca ggcagggtcc gcaggcgcgc 16140
ggctacggcg gcagcggcgg ccatcgctag catgaccaaa ccacgaagag gcaatgtgta 16200
ttgggtgcgc gacgccgcca ccggccagcg cgtgcccgtg cgcacacgcc cccctcgcac 16260
ttagaagata ctgagcagtc tccgatgttg tgtcccagcg gcgagatgtc caagcgcaaa 16320
ttcaaggaag agatgctcca ggtcatcgcg cctgagatct acggtcctgc ggtgaaggat 16380
gaaaaaaagc cccgcaagat caagcgggtc aaaaaggaca aaaaggaaga agatggtgat 16440
gatgggctgg tggagtttgt gcgcgagttt gccccaagga ggcgcgtgca gtggcgcggg 16500
cgcaaagtgt ggccggtgtt gagaccgggg accacagtgg tctttacgcc aggcgagcgc 16560
tccagcaccg tttccaaacg ctcttatgat gaggtgtacg gggacgatga tattctcgag 16620
caggcggctg atcgccttgg cgagtttgca tatggcaaac gcagccgctc gggagccaag 16680
gaagaggcat tgaccatccc cttggatcat ggaaatccca ccccaagcct caaacccgtg 16740
accctgcaac aagtgctgcc cacgccgcca cgcaagggca tcaagcgcga gggcgaggat 16800
ctgtatccca ccatgcagct gatggtgccc aagcgccaga agctggaaga cgtgctggag 16860
aaaatgaaag tggatcctga aatccagcct gaagtcaaag tgaggccaat caagcaggtg 16920
gcgcccggtt tgggggtaca aaccgtggat atcaagatcc ccaccgagtc catggaaatt 16980
caaaccgaac ccatgaagcc cacctccagc accattgagg tgcagacgga tccttggatg 17040
cccgcgcctg ctcctgttac cactactact cgaagaccta gaagaaagta tggttcagcc 17100
aacctgataa tgccaaacta tgctctgcat ccatcaatca tacccactcc tggctaccgc 17160
ggcactcgct actaccgcag tcacagcacc cgccgacgta aagcacctgc cacccgccgc 17220
cgtcgccgcc gccgtgccac tagcaaactt accccctcgg ctatggtgcg gagagtgtac 17280
cgtgatgggc gcgcagctcc tctgacactg ccgcgcgcgc gctaccatcc tagcattgcc 17340
atttaacaac tctgcctcct tgcagatatg gccctcactt gccgccttcg tattcctatt 17400
gctggctacc gcggaagaaa gtcgcgccgt agaagagcag ggttgtctgg gagcgggatg 17460
cgtcgccacc ggcggcggcg cgccatcagc aaacggttgg ggggtggatt tcttcccgct 17520
ttgattccca tcatcgccgc ggcgatcggc gcgataccag gcatagcttc cgtggcggtg 17580
caggcctcgc agcgccactg acattggaaa aagatatctt ataaataaaa atagaatgga 17640
ctctgacgct cctggtcctg tgatatgttt ttgtagacga gatggaagac atcaattttt 17700
catccctggc tccgcgacac ggcacgcggc cgtatatggg cacctggagc gacatcggca 17760
acagccaact gaacggggga gccttcaatt ggagcagtct atggagcggg cttaaaaatt 17820
ttgggtccac tataaagact tatgggaaca aagcttggaa cagcagcaca gggcatgcgc 17880
tgagacaaaa gcttaaagat cagaatttcc aacagaaggt ggtcgatggt atcgcctctg 17940
gaatcaatgg ggtggtagat ctggccaacc aggccgtgca gaaacagatt aacagtcgcc 18000
tggacccggc tcccccagct cctattcatg agttaatgca agtggaggaa gagctccctt 18060
cattggaaaa gcggggcgat aagcgacctc gtccagatat ggaggaaacg ctgctgacca 18120
aggtggatga gccgccctcc tatgaagagg ctgtaaaact gggaatgccc actacaaagc 18180
ccattatgcc tctggccact ggagtgatga agccatctca gtctaaacct gcagttgctg 18240
ctacattgga cttgcccgct cccgtggcca cccccaaacc tgtcgccgcc ccgaagccca 18300
ccgccgtgca acccgtggcc gtggccagac cgcgtcccgg tggtcggccg aatgcaaact 18360
ggcagagcac tctgaacagc atcgtgggtt tgggagtgca cagtgtgaag cgccgtcgct 18420
gctattgatt aaatatggag tagcgcttaa cttgcttgtc tgtgtgtgta tatgtcgatg 18480
ccgcccgccg tgctacagca aagagagaag gagaagaggc gccgctgagt tcctttcaag 18540
atggccaccc catcgatgct gccccagtgg gcgtacatgc acatcgccgg acaggacgct 18600
tcggagtacc tgagtccggg tctggtgcag ttcgcccgcg ccacagatac ctacttcaat 18660
ctggggaaca agtttaggaa ccctaccgtg gctcccaccc acgatgtgac caccgaccgt 18720
agccagcgcc tgacgctgcg ctttgtgccc gttgaccggg aggacaatac ctactcctac 18780
aaagtcagat acaccctggc tgtgggagac aacagggtgt tggatatggc cagcacctac 18840
tttgacatca ggggcgtgtt ggacagagga cctagcttca aaccatactc tggcactgcc 18900
tacaactccc tggctccaaa aggagctcca aactccagtc agtggcaaca aaaggaaaac 18960
aatggtcaag gtgatgcaaa gactcacacc tatggtgtag ctgccactgg aggtattgac 19020
attgacaaaa atggtcttca aattggaatc gatgaaacta aagaagataa taacgaaatt 19080
tatgcagaca aaacattcca acctgaacct caaattggag aagaaaactg gcaagatagc 19140
gaaaactatt atggaggcag ggctcttaaa ccggaaacca agatgaagcc ttgctatggt 19200
tccttcgcta gaccaactaa tgcaaaggga ggtcaagcca aaattaaacc agctcaagag 19260
ggtcaacagt ctatagatta tgacatagac ctggctttct ttgatattcc aagcactggc 19320
ggaggcaatg gcacaaatgt aaatgacaag ccagatatgg ttatgtatac tgaaaatgta 19380
aatctggaaa ctccagacac tcatcttgtt tacaagccag gaacttcaga tgacagttcc 19440
gaggccaatt taactcagca agccatggct aacagaccca actatattgg gtttagagat 19500
aactttattg gcgtcatgta ctacaacagc actggcaaca tgggagtgct tgctggtcaa 19560
gcatcccagc taaatgctgt ggtggacctg caagacagaa acaccgagct gtcttatcag 19620
ctattacttg actctctggg cgacagaacc aggtatttta gtatgtggaa tcaggcggtg 19680
gacagctatg atcctgatgt gcgcattatt gaaaaccatg gtgtggaaga tgaattgcca 19740
aactattgct tcccattgga cggagctggc actaatgctg tttaccaagg agttaagaca 19800
aaagaggata ataatggcga atgggaaaca gacacaaatg ttgcatcgca gaatcagata 19860
tgcaagggca acatatatgc tatggagatc aacctgcaag ccaacctgtg gaaaagtttc 19920
ctttactcca acgtggctct gtacctacca gactcctaca agtacactcc atccaacgtg 19980
acactcccta ccaacactaa cacctatgac tacatgaatg gcagggtggt gtctccatcc 20040
ctggtggatg cctacattaa cattggcgcc aggtggtctc tggatgccat ggacaatgtc 20100
aaccctttca accaccaccg caatgccggc ctgcgctacc ggtccatgct tctgggcaac 20160
ggccgatacg tgcccttcca catccaagtg ccccagaaat tcttcgctat caagaacctg 20220
ctgcttctcc caggctcata cacctacgag tggaacttcc gcaaggatgt caacatgatc 20280
ctgcagagtt cccttggcaa tgacctcaga accgatgggg ccaccatcca gtacaccagc 20340
atcaatctct atgccacctt cttccccatg gctcacaaca ctgcctccac cctggaagcc 20400
atgctgcgca atgacaccaa tgaccagtcc ttcaatgact acctctcagc tgccaacatg 20460
ctttacccca tccctgccaa tgccaccaac gtgcccatct ccatcccatc tcgtaactgg 20520
gctgccttca ggggctggtc tttcacccgc ctcaagacca aggagacccc atctctggga 20580
tcagggttcg atccctactt cgtctactca ggctccattc catacctgga tggaactttc 20640
taccttaacc acactttcaa gaaagtctcc atcatgtttg actcttctgt cagctggcca 20700
ggcaatgaca ggctgctgac tcccaatgag ttcgaaatca agcgcactgt tgatggggaa 20760
gggtacaatg tggcacaatg caacatgacc aaagactggt tcctggttca gatgctctcc 20820
cactacaaca ttggctacca gggcttctac atcccagaag gatacaagga ccgcatgtac 20880
tccttcttca gaaacttcca gcccatgagc cgccaggtgg tcgatcaggt caactacaaa 20940
gactacatgg cagtcaccct tgcctatcag cacaacaact ctggctttgt gggctacctc 21000
gcgcccacca tgcgacaggg ccaaccctac cctgctaact acccataccc gctcattggc 21060
aagactgcag tcaacagtgt cacccagaaa aagttcctct gcgacagggt catgtggcgc 21120
atccccttct ccagcaactt catgtccatg ggggccctta ccgacctggg gcaaaacatg 21180
ctttatgcca actccgccca cgcgctagac atgaatttcg aagtagaccc catggatgag 21240
tccacccttc tctatgttgt cttcgaagtc ttcgacgtgg tcagagtgca ccagccccac 21300
cgcggcgtca tcgaagctgt ctacctgcgc acccccttct cagctggtaa cgccaccaca 21360
taagcgcctt gcttcttgca agtggctgca gcagcatggc ctgtggatcc tccactggat 21420
ccaatgagca agagctcagg gccatcgcca tagacctggg ctgtggaccc tatttcctgg 21480
gaacctttga caagcggttt ccaggcttca tggctcctga caagctcgcc tgtgccattg 21540
tcaacacggc agggcgcgag actggtggtg agcactggct ggcttttgga tggaaccccc 21600
gctccaatac ctgctatctc tttgacccgt ttgggttttc agacgagcgc ctcaagcaga 21660
tctatcaatt cgagtacgag gggctcctgc gccgcagtgc cctggctact aaggaccgat 21720
gcatcactct ggaaaagtct acccagaccg tgcagggtcc gcgctcggct gcctgcgggc 21780
tcttctgctg catgttcctc catgcttttg tgcactggcc cgaccgcccc atggacaaca 21840
accccaccat gaatttgctg acgggggtac ccaacaacat gctccaatcg ccccaagtag 21900
agcccaccct gcgccacaac caggaggcac tctatcgctt cctgaactcc cactcatctt 21960
actttcgttc taaccgcgcg cgcattgaga aggccactgc cttcgatcga atgaataata 22020
acatgtaaac caaattgtgt gtggctcaaa taaacagcac tttattgttt acatgcactg 22080
aggctctggg atgatcattt tttaaaaatc gaaggggttc tggcgggaat cagcatggcc 22140
agatggcagg gacacgttgc ggaactggaa cttgttctgc cacttgaact cgggaatcac 22200
cagcctggga actggaatct ctggaaaggt atcttgccat agctttctgg tcagttgcag 22260
agcgccaagc aggtcaggag cagatatctt gaaatcacag ttggggccag aattctgggc 22320
gcgggagttg cggtacactg ggttgcagca ctggaacacc ataagggcag ggtgtctcac 22380
gctcgccagc acggtctcgt cactgatgca agacacatcc aggtcttcag cattggccat 22440
tccaaagggg gtcatcttgc aggtctgtct gcccatcacg ggagcgcagc caggtttgtg 22500
gttgcaatca caatgaaggg ggatcagcat catcttggcc tggtcggggg taatccctgg 22560
gtaaacagcc ttcatgaagg cttcatactg cttgaaagct tcctgggctt tggttccctc 22620
ggtgtagaac actccacaag acttgctgga aaactgatta gtagcgcagt tggcatcatt 22680
cacacagcag cgggcgtcgt tattagccag ctggaccaca ttcctgcccc agcggttctg 22740
ggtgatcttg gctcgatctg ggttctcctt caacgcgcgc tggccgttct cgctcgccac 22800
atccatctca atgacatgtt ccttctggat catgatgttg ccatgcaggc atctaatctt 22860
gccttcataa tcagtgcagc catgaggcca cagcgcgcac ccggtgcact cccaattgtt 22920
atgggggatc tgggaatggc tatgaaccag cccttgcagg aatcttccca tcatcacagc 22980
cagggtcttt atgctggtaa aggtcagcgg gataccgcgg tgctcctcgt tcacatactg 23040
ctggcagatg cgtctgtagt gctcggcctg ctcgggcatc agcttgaaag aggttttcaa 23100
ctcattatcc agcctgtatc tctccatcat gatggacatt acttccatgc ccttctccca 23160
ggcagaaaca atagggagac tcaggggatt cttgacagta gagacaacct tacttaaggg 23220
gtcatcactg ccaatctttt cgatgcttct cttgccatcc ttctcggtga tgcgcaccgg 23280
cgggtagctg aatcccacag ccaccaactg agcctcttcc ctttcgtctt cgctgtcttg 23340
actgatgtct tgcagaggaa catgtttggt tttcctgggt ttcttcttgg gcggcagctc 23400
tggaggactc tggctccgtt ccggagaccc catggatgag cgagagttgt cgctcaccac 23460
ttggatctgg ctgcctgtag aagaactgga ccccacgcgg cggtaggtgt tcctcttggt 23520
aggcagaggt ggaggcgacg ggctccggtc cggtctgggt ggcggatggc tggcggagcc 23580
ccttccgcgt tcgggggtgc gctccagatg gcggtcgtct gactgacctc cgcggctggc 23640
cattgtgttc tcctaggtag agaaacaaga catggagact cagccatcgc tgccatcgcc 23700
atccaccacc acaagcaccg ccgaggagga ggagtgttta accaccccac catgcagccc 23760
cgctaccacc accagcaccc ttgaaagcga ggtcgacacg gtcgtggagg atttacaggc 23820
tatggaagat attgaggcag ctgtcgagca agaccccggc tatgtgacac cggcggagca 23880
tgatgaggat ctagcgcgct ttctcgacgg tgtggagaaa gcgaaacaag atgaggacga 23940
ggaagaggca gaagcacaac catcggtggc cgactacctc accggcctag ggctagaaga 24000
cgtgctgctt aagcatcttg caaggcagac agtcatagtc aaagacgccc tgctagagcg 24060
ctccgaggtg ccactcagtg tggaagacct cagtcgcgcc tatgagctaa acctcttctc 24120
gcctcgcaag ccccccaagc gtcagcccaa cgggacctgt gagcccaatc cgcgcctcaa 24180
cttctatcca gccttcactg tgcccgaagt actagctacc taccacatct ttttcaagaa 24240
ccaaaagatc cccatctcct gccgcgccaa tcgcacccgc gcagatgccc tactcaactt 24300
ggggcccggc gctcgcatac ctgatatcgc ttccttggaa gaggttccta agatctttga 24360
gggtctgggc aatgaggaaa ctcgggcagc aaacgctctg caaagagaaa cagatgatgg 24420
tgaacaccac agcgctctgg tggagctcca gggcgacaac gctcgtcttg cagtcctcaa 24480
acgcagcatc gaggtcaccc atttcgccta ccccgcactt aatctcccac ccaaagtcat 24540
gagctcggtc atggacacgt tgctcatgaa gcgcgcgagc cccatctccg aggatcagaa 24600
catgcaggac cccgatgcct cagatgaagg caagcctgta gtcagcgacg agcaactggc 24660
tcgctggcta ggctctgact ccccccagtc tttggaggag cggcgcaagc ttatgatggc 24720
agtggtcctg atcacagcgg agctggagtg tctccgccgc ttcttcactg acccagagac 24780
cctgcgcaag cttgaggaga acctgcatta cacattcagt catgggttcg tgcgccaggc 24840
gtgcaagatc tccaacgttc aactcaccaa cctggtctcc tacctgggca tcttgcatga 24900
aaaccggctg gggcagaacg tgctccacac caccctgaag ggggaggccc gccgcgacta 24960
tatccgcgac tgtatctacc tctacctatg ctacacctgg caaagcggga tgggtgtgtg 25020
gcaacagtgc ttggaagagc aaaatctaaa agagctggaa aagctgcttc agaaatctct 25080
taaatctctg tggaccgggt tcgatgagcg gaccaccgct tcggacatgg ccgatattat 25140
cttccccgag cggctcagac acactctgcg cgacgggctg cctgactttg ccagccagag 25200
catgctacaa aactttaggt cattcatctt ggaacgctcc gggatcctgc ccgccacttg 25260
ctgcgcactg ccctccgatt ttgtgcccat cacctaccgg gagtgccccc cgccgctatg 25320
gagccactgc tacctgttcc gcctggccaa ctacttggcc taccactctg atgtgataga 25380
agatgttagt ggcgaagggc tcctggagtg ccactgccgc tgcaacctct gcacccccca 25440
ccgctccctc gcctgcaatc cccagctgct gagcgaaacc cagatcatcg gcaccttcga 25500
gttgcaaggt cccagcggcg aaggcgaggg gtcctctccg gggcaaagtc tgaaactgac 25560
tccggggcta tggacctccg cttaccttcg caagttcgcc cccaaagact accaccccta 25620
tgagatcagg ttttatgaag accaatcaca gccccccaag gccgaactga cggcctgcgt 25680
catcacccag ggggcaatct tggcccaatt gcaagccatc caaaaatccc gccaagaatt 25740
tttgctgaaa aagggacacg ggatctatct agacccccag accggtgagg agctgaatac 25800
acgcttccct caggatgccc cgaggaggca agagaatgaa agttcagatg ccgcccgagg 25860
aggagctgga agactgggac agtcaggcag aggaggaaga ctgggacagc caggcagaag 25920
aggaggacag cctggaggag gacagtctgg aggaaggcga ggagcccaag gaagaggcag 25980
ccgccgccag accatcgtcc tcggcggtgg agacaagcaa ggtcccagac agcacggcta 26040
ccacctccgc tccagctcaa ggggccgctc ggcgacccaa cagtagatgg gacgagacgg 26100
gtcgcttcca gaaccccacc accgtcaaga ccggtaagca ggagcggcag ggatacaagt 26160
cctggcgggg gcataaaagt gccatcatcg cttgcttgca ggagtgtggg ggcaatatat 26220
cctttgccag acgctacctg ctattccatc acggggtgaa tttcccccgc aacatcttgc 26280
attactaccg tcacctccac agcccctact accagcagca agagacagca gaggaaacca 26340
gcggcaactc cgagagttag aaaaccagca gctaaaaaat ccacagcggc ggcagcaggt 26400
gcaggcggac tgaggatcac cgcgaacgag ccagctcaga ccagggagtt gaggaatcgg 26460
atctttccca ccctctatgc catattccaa caaagtcggg gtcaggaaca agaactgaaa 26520
gtaaaaaaca gatctcttcg ctcgctcacc cgcagttgtt tgtatcacaa gagcgaagac 26580
caacttcagc gcactctcga ggacgccgag gctctcttca acaagtactg cgcgctgact 26640
cttaaagagt agactgcgcg cgcttggcga gaaaaggcgg gaattacgtc acctcttggc 26700
cacacctgtg cttcattatg agtaaagaaa ttcccacgcc ttacatgtgg agctatcagc 26760
cccagatggg attggccgct ggcgccgccc aggactactc cacccgcatg aattggctca 26820
gcgccggtcc cgcgatgatc tcacgggtta atggtgtgag agagcaccga aaccagatac 26880
tcctagaaca gtccgccctc accgccactc cccgcaatca cctcaacccc cgtaattggc 26940
ccgccgccct ggtgtaccag gaaactcctg ctcccactac agtactactt cctcgtgacg 27000
cccaggccga agttcagatg actaactcag gtgtacagct ggcgggtggt gccaccctgt 27060
gtcgtcaccg gccaagaccg ggtataaagg gcctggtgat cagaggccga ggtattcagc 27120
tcaacgacga gtcggtgaac tcttcgcttg gtctgcgacc agacggcatc ttccaaatag 27180
ctggttgtgg gagatcttcc ttcactcctc gtcaggctgt cctgactttg gagagttcgt 27240
cctcgcagcc ccgctcgggc ggcatcggga ctctccagtt cgtggaggag tttactccct 27300
cggtctactt caaccccttc tccggttctc ctgggcttta cccggacgag ttcatcccga 27360
actacgacgc catcagtgaa gcggtcgacg gctacgatta atgtctaatg gtggcgcggc 27420
tgagctagct cgactgcgac acctagacca ctgccggcgc tttcgctgct ttgctcggga 27480
tctctgcgag ttcatctact tcgagtaccc tgacgaacat cctcagggac ctgcccacgg 27540
agttcggatt accattgaag gggctatcga ttctcacctg cttcggatct tcaccgctcg 27600
gccagtgcta gttgagcgca accagggcga caccaccatc tccctctgct gcatttgtga 27660
caaccccgga ttgcatgaaa gcttttgttg tcttctttgt actgagtata ataaaagctg 27720
aaattagaga ctactccgga ctctcttgtc gtctgaacaa caccaaccag acccttcact 27780
tcagcgggaa ccagactact cttcactgta aggcttataa ctataagtat cttacttgga 27840
tatacaaagg aacaccgttt gctgtggtaa acaggtgctc caacgacggt gttctcctca 27900
ccttcctagg caacttctcc aactttacct tttctgttcg cagaaacaag cttaccctcc 27960
ttcagcccta ctttcctggg atctatacct gcctcagtgg accttgcaac cacacttttc 28020
acctgattga aaactctacc cttaccttcc cagcgccaat ccctactaac agctcggagt 28080
ccaactcttc cattaccgct gatactaaca ctcctaaaac cggaggtgag ctccgcagcc 28140
ttcccccggc tgcagataac ccttgggtgg tagcgggatt tgtagcgcta ggaatagttg 28200
cgggtgggct cgcgttcgtc ctctgctacc tataccttac ctgctgctca tatttagtag 28260
tactgtgctg ttggtttaga aaatgggggc gctactaatc acacttgctt tactttcgct 28320
tttgggtctg agctcggcta atagcgagaa accaagctgt ctagaaacaa actctccaga 28380
ctgtgtggtt cctcatgggc tctcagaccc agctgatgat ccatgcttaa cttttgaccc 28440
agaaaaaaac tgctcggtga ctatgcagcc ctatgcttac atgtgcacat ctgttataaa 28500
gtgcggatgg ggctgtaaac cgattgaaat tacccacaaa ggcaaaacct ggaataatag 28560
tttgtttaac acatggcagc ctggagacga gcagtggtat acggccggcc actggtggag 28620
atgactgacc ccatggaaaa ctcctctgcc aacgacctgg acatggacgg ccgttcatct 28680
gagcagcgac tggtccagat gcgcattcgc cagaagcagg aacgcgccgc cagagagctc 28740
aaggatgcca ttgaaattca cctgtgcaag aagggcatct tttgcttggt taagcaagca 28800
aagatttctt atgaaatcac tgacaacgac caccgcctgt attatgagct cggtccacag 28860
cggcagaaat tcacctgcat ggttggagtc aaccccatag tcatcactca gcaggctgca 28920
gaaattaaag ggtgcatcca ctgttcctgt gattcccaag aatgcgtcca caccatagtc 28980
aagaccctct gcggccttcg agatcttctt ccaatgaact aaccccttcc cccaacccaa 29040
taaaacattg gttttaatca taataaaaaa tcacttactt taaatctgaa acagtgtctc 29100
cgtccaagtt ttcttgtagc accacttcac tcccctcttc ccagctctgg tactgcaagc 29160
cccggtgggc tgcaaacttt ctccacacct taaaagggat gtcaaattcc tcttgtccaa 29220
caatcttcat tgtctcttcc tagatgtcca caaagcgcgc gcgggtggaa gatgactttg 29280
accctgtcta cccatacgat gctgagctgg caccgtctgt acccttcatc gcccctccct 29340
tcgtttcgtc agacggattt caagaaaaac ccctgggagt tctgtcccta agactagcca 29400
acccagtcac tactaaaaat ggggaactca cacttaaact gggagatggg gtgggcatag 29460
actcagatgg aaacctcaca gcacagacag ttactaaagc aacatccccc cttactgttt 29520
ccaataacgc aattgcactt aacatggaca aaccttttta cagtagcaat ggaaaactat 29580
ccttacaagt tacatcacca ttaaagatag tcgactcttt aaatacattg gctattggct 29640
atgggcaagg cttaggacta aacaatagtg ctcttgctgt gcaattagca tctcccctta 29700
catttgacag caacagcaaa attaaaataa atttgggaag cgggccatta aaaattaatg 29760
cgaataaact gtcaattaac tgcctaaggg gtgtatatgt aacaactgac ggaacttcca 29820
ttgaaacaaa tataagctgg gcaaaaggaa tgaggtttga aggtaatgcc atggctgtaa 29880
acgttgacag caccaaaggt ctacaatttg gcactaccag cacagaatca ggagtcacta 29940
acgctttccc tatccagtta aagattggat ctggtcttag ttttgacagc acaggagcac 30000
ttgtagcttg ggataaggat aatgacaagc ttacactgtg gacaaccgct gacccatcac 30060
ctaattgtac catatataca gacaaggatg ctaaacttac actttgtctt acaaaatgtg 30120
gcagtcaaat actaggcagt gtttcagtac tggctgttaa agctggaacc ctacagccaa 30180
tcagtgaaaa aataggtact gctttggttt cactaaaatt taataacaac ggtgtattgt 30240
taagcaactc cacattaagt aatgaatact ggaactacag gaagggagat gtcacaccag 30300
ccgaagccta tactaatgct gtgggtttta tgccaaacat caaggcatat cctaaaaaca 30360
caaactctgc ctcaaaaagc cacattgtag gacaagtgta ccttaatgga gatgaaacta 30420
aaccaatgca tttaatcatt acatttaatg aaaccagtga tgaaacatgc acatattcca 30480
taacgttcca atggaaatgg aacattggaa catacaccag cgacaccctt gcaacaagct 30540
cctttacctt ttcttacatt gcccaagaat aaaaactgca gacaacaata aagtttaaat 30600
gttttattta aacagtttca cagaacccta gtattcaacc tgccacctcc ctcccaacac 30660
acagagtaca cagtcctttc tccccggctg gccttaaaaa gcatcatatc atgggtaaca 30720
gacatattct taggtgttat attccacacg gtttcctgtc gagccaaacg ctcatcagtg 30780
atattaataa actccccggg cagctcactt aagttcatgt cgctgtccag ctgctgagcc 30840
acaggctgct gtccaacttg cggttgctta acgggcggcg aaggagaagt ccacgcctac 30900
atgggggtag agtcataatc gtgcatcagg atagggcggt ggtgctgcag cagcgcgcga 30960
ataaactgct gccgccgccg ctccgtcctg caggaataca acatggcagt ggtctcctca 31020
gcgatgattc gcaccgcccg cagcataagg cgccttgtcc tccgggcaca gcagcgcacc 31080
ctgatctcac ttaaatcagc acagtaactg cagcacagca ccacaatatt gttcaaaatc 31140
ccacagtgca aggcgctgta tccaaagctc atggcgggga ccacagaacc cacgtggcca 31200
tcataccaca agcgcaggta gattaagtgg cgacccctca taaacacgct ggacataaac 31260
attacctctt ttggcatgtt gtaattcacc acctcccggt accatataaa cctctgatta 31320
aacatggcgc catccaccac catcctaaac cagctggcca aaacctgccc gccggctata 31380
cactgcaggg aaccgggact ggaacaatga cagtggagag cccaggactc gtaaccatgg 31440
atcatcatgc tcgtcatgat atcaatgttg gcacaacaca ggcacacgtg catacacttc 31500
ctcaggatta caagctcctc ccgcgttaga accatatccc agggaacaac ccattcctga 31560
atcagcgtaa atcccacact gcagggaaga cctcgcacgt aactcacgtt gtgcattgtc 31620
aaagtgttac attcgggcag cagcggatga tcctccagta tggtagcgcg ggtttctgtc 31680
tcaaaaggag gtagacgatc cctactgtac ggagtgcgcc gagacaaccg agatcgtgtt 31740
ggtcgtagtg tcatgccaaa tggaacgccg gacgtagtca ttctcgtact ttgagtggca 31800
aaaccttgct ctcgaacagc acacgtctcg tcgcctcctg tcccttctct tcgccttttc 31860
agtgtgatag ttgtaataca gccattcacg aagctcagtc agaagatctt cagcgtctgt 31920
tgtcaaaaac aatccatcca atctgattgc tttcaaaaca tcacaaacag tcgaataagc 31980
caaacccatc caggcaatgc aattattttg gttatccaca atgggagggg gcggaagaca 32040
tggaagaggc ataattaatt ttttaatcca atcgatcacg cagcacttca aaatgaagat 32100
cgcgaaggtg acacctttca cccccactgt gttgatgaaa aataacagcc aagtcaaaat 32160
tgatgcggtt ttcaaggtgc tcgactgtag catcaagcag agcttccaca cgcacgtcca 32220
caaataacag aatagcaaaa gcgggaggag gaagtaaatc ctcaatcatc atagtacagt 32280
ccatcaccat ccctaaataa ttttcatcct tccagccttg gactatattt ttaaactgct 32340
cttgtaaatc caaaccacac atgtggaaaa gttcccaaag agctccctca actaccattc 32400
ttaagcacac cttcatagtg acaaaatatc ttgttcctct gtcacctgca gcaaattaca 32460
aagtccaata ttaggatcta tgcccagaga tctaagctca tccctcaatt ccaactgtaa 32520
aaaggcttcc agatctgccc taacttgttc agccagtggg ctccctggaa taagcgtggg 32580
agaagccaaa ctgcaaaaca gacgcatgcc gccataatta ccaccagaaa acactacgtt 32640
acagtatgca tgctgattca ttccagtaat ttcatccagt gtattggata caaaaaaagg 32700
caagcactct ctcactaatt gtattatgga gacattatca cacaggtaac aatttaaagg 32760
ttgtggaaca ataatgcagt aagtaaccac ggtgcgctcc aacatggtta gtaattttta 32820
gttctgaaaa acaaaacata caaaaaatta tatcatactc atttggcgaa ctggtggaaa 32880
aatgacccta tctagcacaa ggcaagccac tggatcacca atgcgcccct cataaaacct 32940
gtcatcatga ttaaaaagca acaccgaaag ctcttcccta tgtcctgcat gaatgattct 33000
agctgaggaa tataagccag cgcaattagt atctgttaaa gaaaaaaaac ggccaacata 33060
gcctctagga attagcacac ttaatcttaa agacattact gccatccccc ttggatttaa 33120
ggtaaaattt acaggagcat agaaaatata ctgatttccc tcctgcacag gcagcatagc 33180
accaggtccc tctaaaaaca cacacaaagc ttctgcagcc atagcttacc gcgcaaacca 33240
ggcacagcag tgagctaaaa ggacaaagct ctaactcact agccaacctg gcgcacaata 33300
tatagttagt ccttacactg acgtaaccga ccaaagtcta aaaaccccgc caaaaataca 33360
cacacgccca aaaaacgccc cgtgagtcaa aaaacagttt cacttcctcg ttacacccaa 33420
aacgtcgtca cttccggatt cccacggttc gtcacttccg gagctccttg cttaattaac 33480
cccgcccaaa acgtcatcgt ccgcgtcacg ccgccccgcc ccgcgaccgt tgaccccggg 33540
ccaatcaccg cacatcccgc aaaattcaaa ctcgtctaat ttgcatattg gcacactgcc 33600
catataaggt atattattga tgatgattta aatcatatgc ggtgtgaaat accgcacaga 33660
tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg 33720
cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta 33780
tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc 33840
aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag 33900
catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac 33960
caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc 34020
ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt 34080
aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc 34140
gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga 34200
cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta 34260
ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta 34320
tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga 34380
tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg 34440
cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag 34500
tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc 34560
tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact 34620
tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt 34680
cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta 34740
ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta 34800
tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc 34860
gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat 34920
agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt 34980
atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg 35040
tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca 35100
gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta 35160
agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg 35220
cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact 35280
ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg 35340
ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt 35400
actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga 35460
ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc 35520
atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa 35580
caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt 35640
attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa 35700
ttgatttaaa t 35711
<210> 21
<211> 36702
<212> DNA
<213> 人工序列
<220>
<223> pBLY6.RSVF-2A-GLuc
<400> 21
catcatcaat aatatacctt atatgggcag tgtgccaata tgcaaattag acgagtttga 60
attttgcggg atgtgcggtg attggcccgg ggtcaacggt cgcggggcgg ggcggcgtga 120
cgcggacgat gacgttttgg ggaggaggag ctatgttgca agtaatcgtg ggaaatgcga 180
cgtaaaacga ggtggagttt aaacacggaa gtagacaatt ttcccgcgct gtttgacagg 240
aaatgatgtg tttttgggcg gatgcaagtg aaaattctcc attttcgcgc gaaaactaaa 300
tgaggaagtg aaattctgag taattctgag gttatcacag ggcggagtat ttaccgaggg 360
ccgagtagac tttgacccat tacgtggagg tttcgattac tctatttttc acctaaattt 420
ccgcgtactg tgtcaaagtc cgtgttttta cgcgatcgct caatattggc cattagccat 480
attattcatt ggttatatag cataaatcaa tattggctat tggccattgc atacgttgta 540
tccatatcat aatatgtaca tttatattgg ctcatgtcca acattaccgc catgttgaca 600
ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata 660
tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga 720
cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt 780
ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt 840
gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca 900
ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt 960
catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt 1020
tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca 1080
ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg 1140
cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat 1200
cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag 1260
cctccgcggt tggccgtgct cttcctgacg ggtaggtgtc ccctaaccta gggagccaac 1320
catcgggggg ccttctccct aaatccccgt ggcccaccct cctgggcaga ggcagcaggt 1380
ttctcactgg ccccctctcc cccacctcca agcttggcct ttcggctcag atctcagccc 1440
acagctggcc tgatctgggt ctcccctccc accctcaggg agccaggctc ggcatttcgt 1500
cgacatggaa ctgctgatcc tgaaggccaa cgccatcacc accatcctga ccgccgtgac 1560
cttctgcttc gccagcggcc agaacatcac cgaggaattc taccagagca cctgtagcgc 1620
cgtgtccaag ggctacctga gcgccctgcg gaccggctgg tacaccagcg tgatcaccat 1680
cgagctgagc aacatcaaaa agaacaagtg caacggcacc gacgccaaaa tcaagctgat 1740
caagcaggaa ctggacaagt acaagaacgc cgtgaccgag ctgcagctgc tgatgcagag 1800
cacccccgcc accaacaacc gggccagacg ggagctgccc cggttcatga actacaccct 1860
gaacaacgcc aaaaagacca acgtgaccct gagcaagaag cggaagcggc ggttcctggg 1920
cttcctgctg ggcgtgggca gcgccattgc tagcggagtg gctgtgtcta aggtgctgca 1980
cctggaaggc gaagtgaaca agatcaagtc cgccctgctg agcaccaaca aggccgtggt 2040
gtccctgagc aacggcgtgt ccgtgctgac cagcaaggtg ctggatctga agaactacat 2100
cgacaagcag ctgctgccca tcgtgaacaa gcagagctgc agcatcagca acatcgagac 2160
agtgatcgag ttccagcaga agaacaaccg gctgctggaa atcacccgcg agttcagcgt 2220
gaacgccggc gtgaccaccc ccgtgtccac ctacatgctg accaacagcg agctgctgag 2280
cctgatcaac gacatgccca tcaccaacga ccagaaaaag ctgatgagca acaacgtgca 2340
gatcgtgcgg cagcagagct actccatcat gtccatcatc aaagaagagg tgctggccta 2400
cgtggtgcag ctgcccctgt acggcgtgat cgacaccccc tgctggaagc tgcacaccag 2460
ccccctgtgc accaccaaca ccaaagaggg cagcaacatc tgcctgaccc ggaccgaccg 2520
gggctggtac tgcgataatg ccggcagcgt gtcattcttt ccacaagccg agacatgcaa 2580
ggtgcagagc aaccgggtgt tctgcgacac catgaacagc ctgaccctgc ccagcgaggt 2640
gaacctgtgc aacgtggaca tcttcaaccc taagtacgac tgcaagatca tgacctccaa 2700
gaccgacgtg tccagctccg tgatcacctc cctgggcgcc atcgtgtcct gctacggcaa 2760
gaccaagtgc accgccagca acaagaaccg gggcatcatc aagaccttca gcaacggctg 2820
cgactacgtg tccaacaagg gcgtggacac cgtgtccgtg ggcaacaccc tgtactacgt 2880
gaacaaacag gaaggcaaga gcctgtacgt gaagggcgag cccatcatca acttctacga 2940
ccccctggtg ttccccagcg acgagttcga cgccagcatc agccaggtca acgagaagat 3000
caaccagagc ctggccttca tcagaaagag cgacgagctg ctgcacaatg tgaatgccgt 3060
gaagtccacc accaatatca tgatcaccac aatcatcatc gtgatcatcg tcatcctgct 3120
gtccctgatc gccgtgggcc tgctgctgta ctgcaaggcc cggtccaccc ctgtgaccct 3180
gtccaaggac cagctgagcg gcatcaacaa tatcgccttc tccaacggac gcgtgaccga 3240
gctgctttac cggatgaagc gggctgagac atattgcccg agacccctgt tggcaatcca 3300
tcctactgag gctcgccaca aacagaaaat cgtggccccc gtcaaacaga cactcaattt 3360
tgacttgttg aaacttgcag gagatgttga gtcaaacccc gggcctatgg gcgtcaaggt 3420
cctgttcgct ctgatttgta tcgctgtcgc tgaagctaag ccaaccgaga ataatgaaga 3480
ctttaatatc gtggccgtgg cttctaactt cgctaccaca gacctggatg cagacagggg 3540
aaagctgcca ggcaagaaac tgcccctgga ggtcctgaag gagatggaag caaatgcccg 3600
gaaagccggg tgcacaagag gatgcctgat ttgtctgagc cacatcaagt gcactcctaa 3660
gatgaagaag ttcatccccg gccggtgcca tacctacgag ggcgataagg aatccgccca 3720
gggaggaatc ggagaggcta tcgtggatat tcccgaaatc cctggcttca aagacctgga 3780
gcccatggaa cagtttattg cacaggtgga tctgtgcgtc gactgtacta ccggatgcct 3840
gaagggactg gcaaacgtcc agtgtagcga cctgctgaag aaatggctgc ctcagcgatg 3900
tgctacattt gccagcaaga ttcagggcca ggtggacaag attaagggag caggaggcga 3960
ctgataattc tagacgagat ccgaacttgt ttattgcagc ttataatggt tacaaataaa 4020
gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 4080
tgtccaaact catcaatgta tcttatcatg tctgcgatcg cgatggcctg tgtttgagca 4140
taatgtactg accaggtgta acgttcatct gggtggtcgt agaggaatgt tcatgccata 4200
ccaatgcaat tttaatcatg tgaggatctt gatggagccg caagcgtttt ccagagtcag 4260
cttgactgga atctttgaca tgtgtgtgga agcatggaag atcttaagat atgatgatac 4320
caaatccaga tgccgcgcat gcgagtgcgg gggcaggcat gccaggttcc aacctgtatg 4380
tgtggaggtg accgaggagc tgagaccaga tcatttggtg ctgacctgca ctggtgcgga 4440
gttcggttcc agtggtgaag aaactgatta aagtgagtag tgggatgtta taaaagtgac 4500
cataaggtga tgtgagatgg acaaatttgg taatttttat gtatttttgt cttgcagcca 4560
tgagtgggag cgcttccttt gaagggggcg tctttagccc ttatctgacg gggcgtctgc 4620
ctcattgggc tggagtgcgt cagaatgtga tggggtctac agtggatgga agacctgttc 4680
agcctgctaa ttcttctact ctgacttatg ctactatgac ttcctcgcct ttggatgcag 4740
ctgcagctgc tgccgcttct gctgccgcca acactgttcg ggggatggcc ttggagatgg 4800
ggtattatgg aactgtagtg gccaacacca ctaccccaaa taaccccaca gccttgaatg 4860
aggacaagct gctagttctc atgtcccagc tggagtcttt gacccaacgc ctgggcgatc 4920
tagctcagca ggtgtcccag ctgaaggagc agactcaagc tgcaattacc actgcgaggg 4980
gaaattaaaa aaattcaaag aatcaataaa taaaccgaga ctttgttgat tttaaagtgt 5040
gtcattcttt atttaatttt tcgcgcgcga tatgccctgg accaccggtc tctatcattg 5100
aggacacggt ggatcttttc tagaacccga tagaggtggg attggatgtt gaggtacatg 5160
ggcataagac catctttggg gtgtagatag ctccactgca gagcctcatg ctccggggtg 5220
gtgttgtata taacccagtc atagcatggg cgttgggcat gatgttgcac aatatcttta 5280
aggaggagac taatggccac tgggagaccc ttggtgtaag tgtttacaaa tctattaagc 5340
tgggacgggt gcatccgagg tgagataatg tgcattttgg attggatttt tagattggca 5400
atgtttcccc ctagatctct cctgggattc atgttatgca agaccactag aacagtgtat 5460
ccggtgcact tagggaattt gtcatgaagt ttggagggga aagcatgaaa aaatttagac 5520
acacccttgt gtcctcccaa gttctccatg cactcatcca taataatggc aatgggccca 5580
tgggcggcgg cacgggcgaa cacgttcctg ggatctgaca catcatagtt gtggtcttgg 5640
gtcaggtcat cataagccat tttaataaac ttggggcgga gggtgccaga ttgggggatg 5700
aatgttccct cgggccccgg aacatagttt ccttcacata tttgcatttc ccaggctttt 5760
agttcagagg gggggatcat gtccacctgt ggagcgatga agaagacggt ctcgggggcg 5820
ggggtgatta agtgggagga cagcaagttc ctaagcagct gtgacttgcc acacccagtg 5880
ggaccgtaga tgacccctat aacaggttgc agatggtagt ttagggaaag acagctgccg 5940
tcctctcgca ggaggggggc gacctcgttc atcatttccc tcacatgcat gttttcccgc 6000
acaagttccg ataggaggcg ctctccaccc agggaaagga gttcttgaag agatgagaaa 6060
tttttcaagg gttttaagcc atcagccatg ggcattttgg agagggtttg ttgcaagagt 6120
tcaaggcggt cccagagttc ggtgatgtgt tctatggcat ctcgatccag catacttcct 6180
cgtttctggg gttgggacgg ctgcgggagt atggaaccag gcgatgggcg tccagcgctg 6240
ccagtgtccg gtccttccac ggtcgcagcg tccgagtcag ggtcgtttcc gtcacggtga 6300
aggggtgcgc gcctggctgg gcgcttgcga gggtgcgctt caggctcatc ctgctcgtgg 6360
agaaccgctg ccgttctgcg ccctgtgcat cggccaggta gcaattaacc atgagttcgt 6420
agttgagcgc ctctgccgcg tggcctttgg cgcgcagctt acctttggaa gtcttctgac 6480
aggtgggaca gtagagacac ttgagagcat agagttttgg ggctagaaag accgattctg 6540
gggagtatgc atcggcccca caggaggcgc agacggtttc gcattccacc agccatgtaa 6600
gatcgggctc gttggggtca aaaacaagtt ttccgccatg ttttttgatg cgtttcttac 6660
ctttgctttc catgagttcg tgcccccgtt gggtgacaaa gaggctgtcc gtgtccccgt 6720
agactgactt tatgggcctg tcctcgagcg gcgtgccgcg gtcctcttcg tagaggaact 6780
cggaccactc tgagacgaaa gcacgtgtcc aggccagcac aaaggaggct atatgggagg 6840
ggtagcgatc gttgtcaacc aaggggtcta ctttttccaa ggtgtgtaaa cacatgtccc 6900
cttcttccac atccaggaag gtgattggct tgtaagtgta tgccacgtga cctggggtcc 6960
cagacggggg ggtataaaag ggggcgggtc tctgctcgtc ctcactgtct tccggatcgc 7020
tgtccaggag cgccagctgt tgaggtaggt attccctctc gaaggcgggc ataacctccg 7080
cactcaggtt gtcagtttct aggaacgagg aggatttgat attgacagtg cctgccgaga 7140
tgcctttcat gagactgtcg tccatttggt cagaaaagac aatctttttg ttatcaagtt 7200
tggtggcgaa ggatccatac agggcattgg aaagcagttt ggcaatggag cgcatggttt 7260
ggtttttttc tttgtctgcg cgctctttgg cggctatgtt gagttggaca tattcgcggg 7320
ccagacattt ccattgtgga aatatggtag ttaattcatc tgggacgatt ctgactttcc 7380
agcctctgtt atgcagggta atcagatcca cactggttgc cacttctcct ctaagtggtt 7440
cattagtcca gcatagtcgc cccccttttc gagaacagaa agggggtagg ggatctagca 7500
tgagttcgtc tggggggtct gcatctatgg tgaaaatccc aggaaggaga tcttcgtcaa 7560
aatagctgat ggtggcgggg tcatccagag acatttgcca ttctcgagca gccagagcgc 7620
gctcgtaggg gttaagggga gtcccccatg gcatgggatg ggtgagtgca gaagcataca 7680
tgccacagat gtcatagaca tagagcggct cttccagaat ccctatgtaa gtgggataac 7740
atcgcccccc tctgatgctg gctcgcacat aatcatagag ttcatgtgag ggcgctagaa 7800
gacccgagcc caggttggtg cggttgggtt tttctgctct gtagaggatc tggcgaaaga 7860
tggcatggga gtttgatgag atggtgggtc tttggaagat gttgaaatgg gcatgaggca 7920
gtcccacaga gtcccttatg aagtgagcat aggagtcttg cagtttggcc accagctcgg 7980
cggtgaccag cacatccaaa gcacagtagt cgagggtctc tttgatgatg tcatagttag 8040
gttccccttt cttttcccac agctcgcggt tgagaaggta ttcttcgcga tccttccagt 8100
actcttcgag ggggaacccg tccttgtctg aacggtaaga acccagcatg taaaattgat 8160
tgacagcttt gtaggcacaa caccccttct ccacggggag tgagtatgct tgcgcggctt 8220
tgcgcagaga ggtgtgagta agggcgaaag tgtccctgac catgactttg aggaactgat 8280
gcttaaagtc tatgtcatcg caggccccct gctcccacag ttggaagtcc actcgctttt 8340
tgtaggcggg attgggcaaa gcgaaagtaa catcgttgaa taggatcttt ccagccctgg 8400
gcatgaagtt gcgagtaatg cgaaaaggct gaggcacttc tgccctgttg ttgataactt 8460
gggcagccaa gacgatctcg tcaaagccgt tgatgttgtg acccacaatg taaagttcta 8520
cgaagcgtgg gcgtcccttg atgtggggca gttttttaag ctcttcgtag gtcaagtcgt 8580
cagggtcagc gattccatat tgctccaaag cccagtcagg caggtgagga ttagcatgaa 8640
ggaaagaggt ccaaagatcc acggccagag ctgtttgtaa gcggtctctg tactgacgga 8700
aatgtcggcc taccgccatt ttttcaggag taacacagta aaaggtgcgc gggtcctttt 8760
cccagcgatc ccattgaagt tgcaaggcta ggtcgtgggc gaggttgacg agctgttcgt 8820
cccccgaaag tttcatgacc agcatgaaag ggacaagctg cttgccaaag gaccccatcc 8880
aggtgtaggt ttccacatcg taggtgagga agagcctttc tgtgcgagga tgagaaccga 8940
tcgggaagaa ctggatttcc tgccaccagt tggaggaatg gctgttgata tgatggaagt 9000
agaactccct acggcgcgcc gagcattcgt gcttgtgctt gtacagacgg ccacagtact 9060
cgcagcgctg cacgggatgc acctgatgaa tgagctgtac ctggcttcct ttgacaagaa 9120
atttcagtgg gaagttgagg cgtggcgtct gcatctcgtg ttgtattacg tcctggctat 9180
tggtctggcc atcttctgtc tcgatggtgg tcatgctgac gagcccgcgc gggaggcagg 9240
tccagacctc cgcgcggacg ggtctgagag cgaggacgag agcgcgcagg ccggaactgt 9300
ccagggtcct gagacgctgc ggagtcaggt cagtagggag agtacatagg tttacttgca 9360
taagtttttc cagggcatgt gggaggtcaa gatgatattt gatttctact ggcgagttgg 9420
tggagacatc gatggcttgc agggtcccgt gcccctgggg tgctaccacc gtcccttttt 9480
ttttcttgat cgggggcggt gttgcttctt gcatggtaag gtcgtcttct agaagcggcg 9540
gcgaggtcgc gcgccgggtg gcagtggcgg ttctggacct ggaggtagag gcggtagagg 9600
tacgtcggcg ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac ttgcgtgagc 9660
gacgacgcgg cggttgacgt cctggatctg acgcctctgg gtgaatgcta ccggacccgt 9720
gagcttgaac ctgaaagaga gttcaacaga atcaatttcg gtatcgttga cggctgcctg 9780
ccgcaggatt tcttgtacgt cgcccgagtt gtcttggtag gcgatctcgg ccatgaactg 9840
ctcgatctct tcttcttgga gatctccgcg gcccgctcgt tctacggtgg cagcaaggtc 9900
gttggagatg cgccccatga gctgtgagaa tgcattcatg cccgcctcgt tccagacgcg 9960
actgtagacc acggctccct cgggatctct ggcgcgcatg accacttggg cgaggtttag 10020
ttccacgtgt ctggtgaaga ccgcatagtt gcagagacgc tggaagaggt agttgagcgt 10080
ggtggcgatg tgctcggtga caaagaaata catgatccag cgacgaagcg gcatctcgct 10140
gatatcgccc agggcttcca accgttccat ggcttcgtaa aagtccacgg cgaagttgaa 10200
aaactgggag ttgcgagcgg acacggtcaa ctcctcctcc agaagacgga tgagctcggc 10260
gatggtggcg cgcacttcgc gctcaaaggc tcccgggatc tcttcctcct cttcttcttc 10320
caactcttcc tccactaaca tctcttctac ttcctcctca ggcggcgggg gtggaggagg 10380
gggcgcgcgg cgacgccggc gacgcacggg cagacgatcg atgaagcgtt cgatcacttc 10440
tccgcggcgg cgacgcatgg tctcggtgac ggcgcgcccg tcctccctgg gtcgcagagt 10500
gaagacgccg ccgcgcagct ccctgaaatg gtgactggga gggtccccgt ttggtaggga 10560
cagggcactg atgatgcatc ttattaattg ccctgtaggg actccgcgca aggacctgag 10620
cgtctcgaga tccacgggat ctgaaaatct ctgaacgaag gcttcgagcc agtcgcagtc 10680
gcaaggtagg ctgagcactg tttcttcggg gcgggctgct gagctagagg gttgtacgat 10740
gctgctggtg atgaagttaa aataggcagt tctgagacgg cggatggtgg cgaggagcac 10800
caggtctttg ggtccggctt gctggatgcg caggcggtcg gccattcccc atgcattatc 10860
ttggcacctg gccagatctt tatagtagtc ttgcatgagt cgctccacgg gcacttcttc 10920
ttcgcccgct ctgccgtgca tgcgtgtgag tccgtaccct ctctgtggtt ggacgagcgc 10980
caggtcggca acgacccttt cggctagaat ggcttgctgc acctgggtga gggtgttctg 11040
gaaatcatca aagtccacaa agcggtggta ggcccccgtg ttgatggtgt aggagcagtt 11100
ggacatgacc gaccagttga ctgtctggtg tcctggtcgt acgagttccg tgtacctgag 11160
ccgcgagtat gcgcgggagt cgaagatgta atcgttgcag gttcgcacca ggtactggta 11220
gccgatgagg aagtgaggcg gcggctggcg gtagagaggc catcgttcgg tggcgggcgc 11280
gccgggcgct aggtcttcta gcatgagacg gtggtatccg tagacgtacc tggacatcca 11340
ggtaataccg gcggcggtgg tggaggcgcg cggaaactct cgcacgcggt tccagatgtt 11400
gcgcagcggc atgaagtagt tcatggtggg cacggtctgg cccgtgaggc gcgcgcagtc 11460
attgatgctc tagatacggg caaaaacgaa agcgttgagc ggttcccttc cgtggcctgg 11520
aggaacgcga acgggttagg tcgcagcgta ccctggttcg agactaaaga aagcgagcaa 11580
ctcgaaccgg cagagtcgcg gctaacgggt attggcaatc ccgtctcgac ccaagccagc 11640
aaatccagga tacggatggg ggcccctttt gtttttcagg gcatgagtca ccggttaagg 11700
tttacaacgg ctgtttcatg cctttagaag tggctcgcgc ccgtagtctg gagaatcaat 11760
cgccagggtt gcgttgcggc gtgccccggt tcgagcctgc agcttgagtc ggccggtgac 11820
cgcggcaaac gagggcgtgg cggccccgtc gtttctaaga ccttgctagc cgacctctcc 11880
agtttacggg aacgagcccc cttttatttt ttttgttttt gccagatgca tcccgtactg 11940
cggcagatgc gcccacagcc cccacagcag cagcagcagg ctggcctacc ttctctacct 12000
cagccgctac ctgcaactac cgcggtggcc gctgtaagcg gggccggaca gcaggcggct 12060
cctcaatatg aattggactt ggaagagggc gagggattgg caagattggg ggcgccctcg 12120
cccgagcgcc acccgcgggt gcagatgaaa aaggacgttc gcgaatctta cgtgcccaag 12180
cagaatctgt tcagagacag aagcggcgag gagcccgagg agatgcgcgc gtcccgtttt 12240
aacgcgggtc gcgagctgcg acaaggactg gatcgaaaac gggtgttgag ggatgatgat 12300
tttgaggtgg atgaaatgac agggatcagc cccgctcgcg ctcacgtggc tgcagctaat 12360
ctggtgacag cttatgagca gaccgtgaag gaggaaagca acttccagaa atcattcaat 12420
aaccacgtgc gcaccctgat cgcacgcgag gaggtgaccc tgggcctgat gcacctgtgg 12480
gatctgctgg aagccatagt gcagaacccc actagcaaac ccctgactgc tcaactgttt 12540
ctggtggtgc agcacagcag ggataatgag gcattcagag aggcgctgct gaatatcact 12600
gaacctgagg ggagatggct gctggatctg gtgaatatcc tgcagagcat tgtagtgcag 12660
gaacgcagct tgcctttgtc cgagaaggtg gcggcgatca attactctgt gctgagtctg 12720
ggcaaatact atgccaggaa gatctacaaa accccttacg tgcccataga caaggaagtg 12780
aaaatagatg ggttttacat gcgcatgacc ctgaaagtgc taaccctgag cgatgacttg 12840
ggagtgtacc gcaacgacag gatgcaccgc gcggtgagcg ccagcaggag gcgcgagctg 12900
agcgacaaag aattaatgca cagcttgcaa cgagccctga cgggagccgg gacggagggg 12960
gagaactact ttgacatggg tgcagacttg cattggctgc ctagtcgcag ggcattggaa 13020
gcggcaggcg atgggcccta tgtagaggaa gtagtagacg aggacgatga ggagggcgag 13080
tacctggaag actgatggcg cgacccgtat ttttgctaga tggaacaggc gccggaccct 13140
gcgatgcggg cggcgctgca gagccagccg tccggcatta attcctcgga cgattggacc 13200
caggccatgc aacgcatcat ggcgctgacg acccgcaacc ccgaagcctt tagacagcag 13260
cctcaggcca accgcctttc ggccatcctg gaggccgtgg tgccctctcg ctccaacccc 13320
acccacgaga aggttctggc catcgtgaat gccctggtgg agaacaaggc catccgctcc 13380
gatgaagccg ggctggtata caacgccttg ctcgagcgcg tggctcgcta caacagcagc 13440
aatgtccaga ctaacctgga caggatggtg accgacgtgc gcgaggccgt gtcccagcgc 13500
gaacggttcc atcgcgagtc taacctgggt tccatggtag cgctgaacgc tttcctcagt 13560
tcccagcctg ccaatgtgcc ccggggacag gaagactata ccaactttat tagcgccctg 13620
agactcatgg tagccgaggt tcctcagagc gaggtgtacc agtccggtcc agactacttt 13680
ttccagacaa gcaggaacgg tatgcagaca gtgaacttaa gccaggcttt caagaacctg 13740
caagggctgt ggggagtcca agctccagtg ggcgacaggg cgaccgtgtc gagcctgttg 13800
actccaaatt cccgtttgct gctgctgctg gtgtccccct tcactgacag cggcagcata 13860
aacagaaact cctacttggg ctacctgata aacttgtatc gcgaagctat aggtcaggcc 13920
cacgtggacg aacagaccta tcaggagatc actaatgtga gtcgcgctct gggccaggac 13980
gaccctggaa acctggaagc tactctaaac tttctgctga ccaaccgctc gcaaaaaatc 14040
cctcctcagt atacattaac tgcggaggag gaacggatct tgagatacgt gcagcagagc 14100
gtgggtctgt tcctgatgca agagggtgcg acccctagcg ccgcgcttga tatgacagcg 14160
cgcaacatgg agcccagcat gtatgccagc aacagaccat tcattaataa attgatggat 14220
tacttccatc gcgcggccgc tatgaactct gattacttca ccaatgctat tctgaacccc 14280
cattggctgc ctccgcctgg tttttatact ggcgagtatg acatgcctga ccccaacgat 14340
gggttcttgt gggacgatgt ggacagcgtg gcgttctcgc ctaccgctcc tcgtactttt 14400
tggaagaagg aaggtagtga cagaagaccc tcctccgtgc tgtcaggacg tgagggtgct 14460
gccgcggcgg tccccgatgc tgcaagcccc tttcccagtc tgccattttc actaaacagc 14520
gtgcgcagta gcgagctggg gagaataacc cgccctcgct tgctgggcga ggacgagtat 14580
ttgaatgact ccctactgag acccgagcgg gaaaagaact tccctaataa tgggattgaa 14640
agcctggtgg ataagatgag cagatggaag acctatgccc aggagcacag agatgagcct 14700
agaatcttgg gtcctacagt aggcacccgc agacgccagc gccatgatag acagcggggt 14760
ctggtgtggg acgatgagga ttctgcagat gacagcagcg tgttggactt gggcgggagg 14820
ggaggtgtgg gcaacccgtt cgcacacttg cgtccccgta ttggacgcat gatgtaaaag 14880
tgaaaataaa aaaggaactc accaaggcca tggcgaccag cgtgcgttcg ttctttctgt 14940
tgttgtatct agtatgatga ggcgcaccgt gctaggcgga tcggtggcgt atccggaggg 15000
tcctcctcct tcgtacgaaa gcgtgatgca gcaggtggcg gcggcggcga tgcaaccccc 15060
cttggaggct ccttacgtgc ccccgcggta cctggcacct accgagggga gaaacagcat 15120
tcgttattcg gaactcacac ccttgtatga caccacccgg ttgtacctgg tggacaacaa 15180
atcggcggac attgcctcgt tgaactatca gaacgaccac agcaacttct tgacaacggt 15240
ggtgcagaac aatgacttta cccccacgga ggccagcacc cagaccatca actttgacga 15300
gcgctcccgg tggggcggtc agctgaagac catcatgcac accaacatgc ccaacgtgaa 15360
cgagttcatg tttagcaaca agttcagggc tagggtgatg gtgtccagaa ccacacctaa 15420
agaggtgaca gtcacaacag actatgatgg tagtcaggac atcttggaat acgagtgggt 15480
tgactttgag ttaccagaag gcaacttctc tgccaccatg accatagacc tgatgaataa 15540
tgcaattgtt gataattacc taaaagtggg tagacagaat ggggtactgg agagtgacat 15600
aggtgttaag tttgacacta ggaactttag gcttggttgg gacccagtga cagagttggt 15660
catgcctggg gtctacacca atgaagcttt ccatcctgac atagtcctac tacctggctg 15720
cggagtggac ttcactgaga gccgcctcag taatctgcta ggcattagaa agaaacagcc 15780
attccaggaa gggttccaga tcatgtatga ggatctggag ggtggtaaca tccccgccct 15840
gcttgatgta aatgcatatg agaagagcaa ggaagataat acaaccacca caaatgaagc 15900
tgtggccgcg gcttcatcta ctgaagccaa agctgtggta gatgcttcca cttcaacaga 15960
aaacaccact gatgaaaaag tcaccagggg agatacattt gccacccctg aacaagagaa 16020
ggcagctgag gcagagtctg atattatgct tctgtccacc gatgaaaacg aaactaaaaa 16080
acaactggtt attcgagcgg tgaccaagga tagtaaggac aggagttata atgtattgtc 16140
agatggaaag aacacagctt accgtagttg gtacctggca tacaattatg gcgaccgtga 16200
gaaaggggtg cgttcttgga cactgcttac cacctcggat gtcacctgcg gcgtggagca 16260
agtctattgg tcgctaccag atatgatgca agatccagtc acctttcgct ccacacgcca 16320
agttagcaac tacccagtgg tgggcgcaga gctgctccca gtgcattcca gaagcttcta 16380
caacgagcaa gccgtctact cgcaacagct ccgccagtac acctcgctca cgcacgtctt 16440
caaccgcttc cccgagaatc agatcctcgt ccgcccgccc gcgccaacca ttaccaccgt 16500
cagtgaaaac gttcctgctc tcacagatca cgggaccctg ccgctgcgca gcagtatccg 16560
gggagtccag cgcgtgaccg ttactgacgc cagacgccgc acctgtccat acgtatacaa 16620
ggccctgggc atagtcgcgc cgcgcgtcct ttcaagccgc actttctaaa aaaatgtcca 16680
ttctcatctc gcccagtaat aacaccggtt ggggcctgcg cacacctagc aagatgtatg 16740
gaggcgctcg cagacgctcc actcagcacc ctgtgcgcgt gcgcgggcat ttccgcgctc 16800
cctggggcgc cctcaaggga cgctctcgta ctaggaccac cgttgacgat gtgatcgacc 16860
aggtggtcgc cgatgcacgt aactataccc ccgcagccgc acctgcatcc accgtggatg 16920
cggtcattga cagcgtggta gccgatgcgc gcgcctatgc tcgcgccaag agcaggaggc 16980
ggcgtattgc caggcgtcac cgagctactc cagccatgcg agctgcaaga gctttattgc 17040
ggagagccag acgtgtgggg cgaagagcca tgcgtagagc ggccagacgc gcggcttcag 17100
gtgccagcgc aggcagggtc cgcaggcgcg cggctacggc ggcagcggcg gccatcgcta 17160
gcatgaccaa accacgaaga ggcaatgtgt attgggtgcg cgacgccgcc accggccagc 17220
gcgtgcccgt gcgcacacgc ccccctcgca cttagaagat actgagcagt ctccgatgtt 17280
gtgtcccagc ggcgagatgt ccaagcgcaa attcaaggaa gagatgctcc aggtcatcgc 17340
gcctgagatc tacggtcctg cggtgaagga tgaaaaaaag ccccgcaaga tcaagcgggt 17400
caaaaaggac aaaaaggaag aagatggtga tgatgggctg gtggagtttg tgcgcgagtt 17460
tgccccaagg aggcgcgtgc agtggcgcgg gcgcaaagtg tggccggtgt tgagaccggg 17520
gaccacagtg gtctttacgc caggcgagcg ctccagcacc gtttccaaac gctcttatga 17580
tgaggtgtac ggggacgatg atattctcga gcaggcggct gatcgccttg gcgagtttgc 17640
atatggcaaa cgcagccgct cgggagccaa ggaagaggca ttgaccatcc ccttggatca 17700
tggaaatccc accccaagcc tcaaacccgt gaccctgcaa caagtgctgc ccacgccgcc 17760
acgcaagggc atcaagcgcg agggcgagga tctgtatccc accatgcagc tgatggtgcc 17820
caagcgccag aagctggaag acgtgctgga gaaaatgaaa gtggatcctg aaatccagcc 17880
tgaagtcaaa gtgaggccaa tcaagcaggt ggcgcccggt ttgggggtac aaaccgtgga 17940
tatcaagatc cccaccgagt ccatggaaat tcaaaccgaa cccatgaagc ccacctccag 18000
caccattgag gtgcagacgg atccttggat gcccgcgcct gctcctgtta ccactactac 18060
tcgaagacct agaagaaagt atggttcagc caacctgata atgccaaact atgctctgca 18120
tccatcaatc atacccactc ctggctaccg cggcactcgc tactaccgca gtcacagcac 18180
ccgccgacgt aaagcacctg ccacccgccg ccgtcgccgc cgccgtgcca ctagcaaact 18240
taccccctcg gctatggtgc ggagagtgta ccgtgatggg cgcgcagctc ctctgacact 18300
gccgcgcgcg cgctaccatc ctagcattgc catttaacaa ctctgcctcc ttgcagatat 18360
ggccctcact tgccgccttc gtattcctat tgctggctac cgcggaagaa agtcgcgccg 18420
tagaagagca gggttgtctg ggagcgggat gcgtcgccac cggcggcggc gcgccatcag 18480
caaacggttg gggggtggat ttcttcccgc tttgattccc atcatcgccg cggcgatcgg 18540
cgcgatacca ggcatagctt ccgtggcggt gcaggcctcg cagcgccact gacattggaa 18600
aaagatatct tataaataaa aatagaatgg actctgacgc tcctggtcct gtgatatgtt 18660
tttgtagacg agatggaaga catcaatttt tcatccctgg ctccgcgaca cggcacgcgg 18720
ccgtatatgg gcacctggag cgacatcggc aacagccaac tgaacggggg agccttcaat 18780
tggagcagtc tatggagcgg gcttaaaaat tttgggtcca ctataaagac ttatgggaac 18840
aaagcttgga acagcagcac agggcatgcg ctgagacaaa agcttaaaga tcagaatttc 18900
caacagaagg tggtcgatgg tatcgcctct ggaatcaatg gggtggtaga tctggccaac 18960
caggccgtgc agaaacagat taacagtcgc ctggacccgg ctcccccagc tcctattcat 19020
gagttaatgc aagtggagga agagctccct tcattggaaa agcggggcga taagcgacct 19080
cgtccagata tggaggaaac gctgctgacc aaggtggatg agccgccctc ctatgaagag 19140
gctgtaaaac tgggaatgcc cactacaaag cccattatgc ctctggccac tggagtgatg 19200
aagccatctc agtctaaacc tgcagttgct gctacattgg acttgcccgc tcccgtggcc 19260
acccccaaac ctgtcgccgc cccgaagccc accgccgtgc aacccgtggc cgtggccaga 19320
ccgcgtcccg gtggtcggcc gaatgcaaac tggcagagca ctctgaacag catcgtgggt 19380
ttgggagtgc acagtgtgaa gcgccgtcgc tgctattgat taaatatgga gtagcgctta 19440
acttgcttgt ctgtgtgtgt atatgtcgat gccgcccgcc gtgctacagc aaagagagaa 19500
ggagaagagg cgccgctgag ttcctttcaa gatggccacc ccatcgatgc tgccccagtg 19560
ggcgtacatg cacatcgccg gacaggacgc ttcggagtac ctgagtccgg gtctggtgca 19620
gttcgcccgc gccacagata cctacttcaa tctggggaac aagtttagga accctaccgt 19680
ggctcccacc cacgatgtga ccaccgaccg tagccagcgc ctgacgctgc gctttgtgcc 19740
cgttgaccgg gaggacaata cctactccta caaagtcaga tacaccctgg ctgtgggaga 19800
caacagggtg ttggatatgg ccagcaccta ctttgacatc aggggcgtgt tggacagagg 19860
acctagcttc aaaccatact ctggcactgc ctacaactcc ctggctccaa aaggagctcc 19920
aaactccagt cagtggcaac aaaaggaaaa caatggtcaa ggtgatgcaa agactcacac 19980
ctatggtgta gctgccactg gaggtattga cattgacaaa aatggtcttc aaattggaat 20040
cgatgaaact aaagaagata ataacgaaat ttatgcagac aaaacattcc aacctgaacc 20100
tcaaattgga gaagaaaact ggcaagatag cgaaaactat tatggaggca gggctcttaa 20160
accggaaacc aagatgaagc cttgctatgg ttccttcgct agaccaacta atgcaaaggg 20220
aggtcaagcc aaaattaaac cagctcaaga gggtcaacag tctatagatt atgacataga 20280
cctggctttc tttgatattc caagcactgg cggaggcaat ggcacaaatg taaatgacaa 20340
gccagatatg gttatgtata ctgaaaatgt aaatctggaa actccagaca ctcatcttgt 20400
ttacaagcca ggaacttcag atgacagttc cgaggccaat ttaactcagc aagccatggc 20460
taacagaccc aactatattg ggtttagaga taactttatt ggcgtcatgt actacaacag 20520
cactggcaac atgggagtgc ttgctggtca agcatcccag ctaaatgctg tggtggacct 20580
gcaagacaga aacaccgagc tgtcttatca gctattactt gactctctgg gcgacagaac 20640
caggtatttt agtatgtgga atcaggcggt ggacagctat gatcctgatg tgcgcattat 20700
tgaaaaccat ggtgtggaag atgaattgcc aaactattgc ttcccattgg acggagctgg 20760
cactaatgct gtttaccaag gagttaagac aaaagaggat aataatggcg aatgggaaac 20820
agacacaaat gttgcatcgc agaatcagat atgcaagggc aacatatatg ctatggagat 20880
caacctgcaa gccaacctgt ggaaaagttt cctttactcc aacgtggctc tgtacctacc 20940
agactcctac aagtacactc catccaacgt gacactccct accaacacta acacctatga 21000
ctacatgaat ggcagggtgg tgtctccatc cctggtggat gcctacatta acattggcgc 21060
caggtggtct ctggatgcca tggacaatgt caaccctttc aaccaccacc gcaatgccgg 21120
cctgcgctac cggtccatgc ttctgggcaa cggccgatac gtgcccttcc acatccaagt 21180
gccccagaaa ttcttcgcta tcaagaacct gctgcttctc ccaggctcat acacctacga 21240
gtggaacttc cgcaaggatg tcaacatgat cctgcagagt tcccttggca atgacctcag 21300
aaccgatggg gccaccatcc agtacaccag catcaatctc tatgccacct tcttccccat 21360
ggctcacaac actgcctcca ccctggaagc catgctgcgc aatgacacca atgaccagtc 21420
cttcaatgac tacctctcag ctgccaacat gctttacccc atccctgcca atgccaccaa 21480
cgtgcccatc tccatcccat ctcgtaactg ggctgccttc aggggctggt ctttcacccg 21540
cctcaagacc aaggagaccc catctctggg atcagggttc gatccctact tcgtctactc 21600
aggctccatt ccatacctgg atggaacttt ctaccttaac cacactttca agaaagtctc 21660
catcatgttt gactcttctg tcagctggcc aggcaatgac aggctgctga ctcccaatga 21720
gttcgaaatc aagcgcactg ttgatgggga agggtacaat gtggcacaat gcaacatgac 21780
caaagactgg ttcctggttc agatgctctc ccactacaac attggctacc agggcttcta 21840
catcccagaa ggatacaagg accgcatgta ctccttcttc agaaacttcc agcccatgag 21900
ccgccaggtg gtcgatcagg tcaactacaa agactacatg gcagtcaccc ttgcctatca 21960
gcacaacaac tctggctttg tgggctacct cgcgcccacc atgcgacagg gccaacccta 22020
ccctgctaac tacccatacc cgctcattgg caagactgca gtcaacagtg tcacccagaa 22080
aaagttcctc tgcgacaggg tcatgtggcg catccccttc tccagcaact tcatgtccat 22140
gggggccctt accgacctgg ggcaaaacat gctttatgcc aactccgccc acgcgctaga 22200
catgaatttc gaagtagacc ccatggatga gtccaccctt ctctatgttg tcttcgaagt 22260
cttcgacgtg gtcagagtgc accagcccca ccgcggcgtc atcgaagctg tctacctgcg 22320
cacccccttc tcagctggta acgccaccac ataagcgcct tgcttcttgc aagtggctgc 22380
agcagcatgg cctgtggatc ctccactgga tccaatgagc aagagctcag ggccatcgcc 22440
atagacctgg gctgtggacc ctatttcctg ggaacctttg acaagcggtt tccaggcttc 22500
atggctcctg acaagctcgc ctgtgccatt gtcaacacgg cagggcgcga gactggtggt 22560
gagcactggc tggcttttgg atggaacccc cgctccaata cctgctatct ctttgacccg 22620
tttgggtttt cagacgagcg cctcaagcag atctatcaat tcgagtacga ggggctcctg 22680
cgccgcagtg ccctggctac taaggaccga tgcatcactc tggaaaagtc tacccagacc 22740
gtgcagggtc cgcgctcggc tgcctgcggg ctcttctgct gcatgttcct ccatgctttt 22800
gtgcactggc ccgaccgccc catggacaac aaccccacca tgaatttgct gacgggggta 22860
cccaacaaca tgctccaatc gccccaagta gagcccaccc tgcgccacaa ccaggaggca 22920
ctctatcgct tcctgaactc ccactcatct tactttcgtt ctaaccgcgc gcgcattgag 22980
aaggccactg ccttcgatcg aatgaataat aacatgtaaa ccaaattgtg tgtggctcaa 23040
ataaacagca ctttattgtt tacatgcact gaggctctgg gatgatcatt ttttaaaaat 23100
cgaaggggtt ctggcgggaa tcagcatggc cagatggcag ggacacgttg cggaactgga 23160
acttgttctg ccacttgaac tcgggaatca ccagcctggg aactggaatc tctggaaagg 23220
tatcttgcca tagctttctg gtcagttgca gagcgccaag caggtcagga gcagatatct 23280
tgaaatcaca gttggggcca gaattctggg cgcgggagtt gcggtacact gggttgcagc 23340
actggaacac cataagggca gggtgtctca cgctcgccag cacggtctcg tcactgatgc 23400
aagacacatc caggtcttca gcattggcca ttccaaaggg ggtcatcttg caggtctgtc 23460
tgcccatcac gggagcgcag ccaggtttgt ggttgcaatc acaatgaagg gggatcagca 23520
tcatcttggc ctggtcgggg gtaatccctg ggtaaacagc cttcatgaag gcttcatact 23580
gcttgaaagc ttcctgggct ttggttccct cggtgtagaa cactccacaa gacttgctgg 23640
aaaactgatt agtagcgcag ttggcatcat tcacacagca gcgggcgtcg ttattagcca 23700
gctggaccac attcctgccc cagcggttct gggtgatctt ggctcgatct gggttctcct 23760
tcaacgcgcg ctggccgttc tcgctcgcca catccatctc aatgacatgt tccttctgga 23820
tcatgatgtt gccatgcagg catctaatct tgccttcata atcagtgcag ccatgaggcc 23880
acagcgcgca cccggtgcac tcccaattgt tatgggggat ctgggaatgg ctatgaacca 23940
gcccttgcag gaatcttccc atcatcacag ccagggtctt tatgctggta aaggtcagcg 24000
ggataccgcg gtgctcctcg ttcacatact gctggcagat gcgtctgtag tgctcggcct 24060
gctcgggcat cagcttgaaa gaggttttca actcattatc cagcctgtat ctctccatca 24120
tgatggacat tacttccatg cccttctccc aggcagaaac aatagggaga ctcaggggat 24180
tcttgacagt agagacaacc ttacttaagg ggtcatcact gccaatcttt tcgatgcttc 24240
tcttgccatc cttctcggtg atgcgcaccg gcgggtagct gaatcccaca gccaccaact 24300
gagcctcttc cctttcgtct tcgctgtctt gactgatgtc ttgcagagga acatgtttgg 24360
ttttcctggg tttcttcttg ggcggcagct ctggaggact ctggctccgt tccggagacc 24420
ccatggatga gcgagagttg tcgctcacca cttggatctg gctgcctgta gaagaactgg 24480
accccacgcg gcggtaggtg ttcctcttgg taggcagagg tggaggcgac gggctccggt 24540
ccggtctggg tggcggatgg ctggcggagc cccttccgcg ttcgggggtg cgctccagat 24600
ggcggtcgtc tgactgacct ccgcggctgg ccattgtgtt ctcctaggta gagaaacaag 24660
acatggagac tcagccatcg ctgccatcgc catccaccac cacaagcacc gccgaggagg 24720
aggagtgttt aaccacccca ccatgcagcc ccgctaccac caccagcacc cttgaaagcg 24780
aggtcgacac ggtcgtggag gatttacagg ctatggaaga tattgaggca gctgtcgagc 24840
aagaccccgg ctatgtgaca ccggcggagc atgatgagga tctagcgcgc tttctcgacg 24900
gtgtggagaa agcgaaacaa gatgaggacg aggaagaggc agaagcacaa ccatcggtgg 24960
ccgactacct caccggccta gggctagaag acgtgctgct taagcatctt gcaaggcaga 25020
cagtcatagt caaagacgcc ctgctagagc gctccgaggt gccactcagt gtggaagacc 25080
tcagtcgcgc ctatgagcta aacctcttct cgcctcgcaa gccccccaag cgtcagccca 25140
acgggacctg tgagcccaat ccgcgcctca acttctatcc agccttcact gtgcccgaag 25200
tactagctac ctaccacatc tttttcaaga accaaaagat ccccatctcc tgccgcgcca 25260
atcgcacccg cgcagatgcc ctactcaact tggggcccgg cgctcgcata cctgatatcg 25320
cttccttgga agaggttcct aagatctttg agggtctggg caatgaggaa actcgggcag 25380
caaacgctct gcaaagagaa acagatgatg gtgaacacca cagcgctctg gtggagctcc 25440
agggcgacaa cgctcgtctt gcagtcctca aacgcagcat cgaggtcacc catttcgcct 25500
accccgcact taatctccca cccaaagtca tgagctcggt catggacacg ttgctcatga 25560
agcgcgcgag ccccatctcc gaggatcaga acatgcagga ccccgatgcc tcagatgaag 25620
gcaagcctgt agtcagcgac gagcaactgg ctcgctggct aggctctgac tccccccagt 25680
ctttggagga gcggcgcaag cttatgatgg cagtggtcct gatcacagcg gagctggagt 25740
gtctccgccg cttcttcact gacccagaga ccctgcgcaa gcttgaggag aacctgcatt 25800
acacattcag tcatgggttc gtgcgccagg cgtgcaagat ctccaacgtt caactcacca 25860
acctggtctc ctacctgggc atcttgcatg aaaaccggct ggggcagaac gtgctccaca 25920
ccaccctgaa gggggaggcc cgccgcgact atatccgcga ctgtatctac ctctacctat 25980
gctacacctg gcaaagcggg atgggtgtgt ggcaacagtg cttggaagag caaaatctaa 26040
aagagctgga aaagctgctt cagaaatctc ttaaatctct gtggaccggg ttcgatgagc 26100
ggaccaccgc ttcggacatg gccgatatta tcttccccga gcggctcaga cacactctgc 26160
gcgacgggct gcctgacttt gccagccaga gcatgctaca aaactttagg tcattcatct 26220
tggaacgctc cgggatcctg cccgccactt gctgcgcact gccctccgat tttgtgccca 26280
tcacctaccg ggagtgcccc ccgccgctat ggagccactg ctacctgttc cgcctggcca 26340
actacttggc ctaccactct gatgtgatag aagatgttag tggcgaaggg ctcctggagt 26400
gccactgccg ctgcaacctc tgcacccccc accgctccct cgcctgcaat ccccagctgc 26460
tgagcgaaac ccagatcatc ggcaccttcg agttgcaagg tcccagcggc gaaggcgagg 26520
ggtcctctcc ggggcaaagt ctgaaactga ctccggggct atggacctcc gcttaccttc 26580
gcaagttcgc ccccaaagac taccacccct atgagatcag gttttatgaa gaccaatcac 26640
agccccccaa ggccgaactg acggcctgcg tcatcaccca gggggcaatc ttggcccaat 26700
tgcaagccat ccaaaaatcc cgccaagaat ttttgctgaa aaagggacac gggatctatc 26760
tagaccccca gaccggtgag gagctgaata cacgcttccc tcaggatgcc ccgaggaggc 26820
aagagaatga aagttcagat gccgcccgag gaggagctgg aagactggga cagtcaggca 26880
gaggaggaag actgggacag ccaggcagaa gaggaggaca gcctggagga ggacagtctg 26940
gaggaaggcg aggagcccaa ggaagaggca gccgccgcca gaccatcgtc ctcggcggtg 27000
gagacaagca aggtcccaga cagcacggct accacctccg ctccagctca aggggccgct 27060
cggcgaccca acagtagatg ggacgagacg ggtcgcttcc agaaccccac caccgtcaag 27120
accggtaagc aggagcggca gggatacaag tcctggcggg ggcataaaag tgccatcatc 27180
gcttgcttgc aggagtgtgg gggcaatata tcctttgcca gacgctacct gctattccat 27240
cacggggtga atttcccccg caacatcttg cattactacc gtcacctcca cagcccctac 27300
taccagcagc aagagacagc agaggaaacc agcggcaact ccgagagtta gaaaaccagc 27360
agctaaaaaa tccacagcgg cggcagcagg tgcaggcgga ctgaggatca ccgcgaacga 27420
gccagctcag accagggagt tgaggaatcg gatctttccc accctctatg ccatattcca 27480
acaaagtcgg ggtcaggaac aagaactgaa agtaaaaaac agatctcttc gctcgctcac 27540
ccgcagttgt ttgtatcaca agagcgaaga ccaacttcag cgcactctcg aggacgccga 27600
ggctctcttc aacaagtact gcgcgctgac tcttaaagag tagactgcgc gcgcttggcg 27660
agaaaaggcg ggaattacgt cacctcttgg ccacacctgt gcttcattat gagtaaagaa 27720
attcccacgc cttacatgtg gagctatcag ccccagatgg gattggccgc tggcgccgcc 27780
caggactact ccacccgcat gaattggctc agcgccggtc ccgcgatgat ctcacgggtt 27840
aatggtgtga gagagcaccg aaaccagata ctcctagaac agtccgccct caccgccact 27900
ccccgcaatc acctcaaccc ccgtaattgg cccgccgccc tggtgtacca ggaaactcct 27960
gctcccacta cagtactact tcctcgtgac gcccaggccg aagttcagat gactaactca 28020
ggtgtacagc tggcgggtgg tgccaccctg tgtcgtcacc ggccaagacc gggtataaag 28080
ggcctggtga tcagaggccg aggtattcag ctcaacgacg agtcggtgaa ctcttcgctt 28140
ggtctgcgac cagacggcat cttccaaata gctggttgtg ggagatcttc cttcactcct 28200
cgtcaggctg tcctgacttt ggagagttcg tcctcgcagc cccgctcggg cggcatcggg 28260
actctccagt tcgtggagga gtttactccc tcggtctact tcaacccctt ctccggttct 28320
cctgggcttt acccggacga gttcatcccg aactacgacg ccatcagtga agcggtcgac 28380
ggctacgatt aatgtctaat ggtggcgcgg ctgagctagc tcgactgcga cacctagacc 28440
actgccggcg ctttcgctgc tttgctcggg atctctgcga gttcatctac ttcgagtacc 28500
ctgacgaaca tcctcaggga cctgcccacg gagttcggat taccattgaa ggggctatcg 28560
attctcacct gcttcggatc ttcaccgctc ggccagtgct agttgagcgc aaccagggcg 28620
acaccaccat ctccctctgc tgcatttgtg acaaccccgg attgcatgaa agcttttgtt 28680
gtcttctttg tactgagtat aataaaagct gaaattagag actactccgg actctcttgt 28740
cgtctgaaca acaccaacca gacccttcac ttcagcggga accagactac tcttcactgt 28800
aaggcttata actataagta tcttacttgg atatacaaag gaacaccgtt tgctgtggta 28860
aacaggtgct ccaacgacgg tgttctcctc accttcctag gcaacttctc caactttacc 28920
ttttctgttc gcagaaacaa gcttaccctc cttcagccct actttcctgg gatctatacc 28980
tgcctcagtg gaccttgcaa ccacactttt cacctgattg aaaactctac ccttaccttc 29040
ccagcgccaa tccctactaa cagctcggag tccaactctt ccattaccgc tgatactaac 29100
actcctaaaa ccggaggtga gctccgcagc cttcccccgg ctgcagataa cccttgggtg 29160
gtagcgggat ttgtagcgct aggaatagtt gcgggtgggc tcgcgttcgt cctctgctac 29220
ctatacctta cctgctgctc atatttagta gtactgtgct gttggtttag aaaatggggg 29280
cgctactaat cacacttgct ttactttcgc ttttgggtct gagctcggct aatagcgaga 29340
aaccaagctg tctagaaaca aactctccag actgtgtggt tcctcatggg ctctcagacc 29400
cagctgatga tccatgctta acttttgacc cagaaaaaaa ctgctcggtg actatgcagc 29460
cctatgctta catgtgcaca tctgttataa agtgcggatg gggctgtaaa ccgattgaaa 29520
ttacccacaa aggcaaaacc tggaataata gtttgtttaa cacatggcag cctggagacg 29580
agcagtggta tacggccggc cactggtgga gatgactgac cccatggaaa actcctctgc 29640
caacgacctg gacatggacg gccgttcatc tgagcagcga ctggtccaga tgcgcattcg 29700
ccagaagcag gaacgcgccg ccagagagct caaggatgcc attgaaattc acctgtgcaa 29760
gaagggcatc ttttgcttgg ttaagcaagc aaagatttct tatgaaatca ctgacaacga 29820
ccaccgcctg tattatgagc tcggtccaca gcggcagaaa ttcacctgca tggttggagt 29880
caaccccata gtcatcactc agcaggctgc agaaattaaa gggtgcatcc actgttcctg 29940
tgattcccaa gaatgcgtcc acaccatagt caagaccctc tgcggccttc gagatcttct 30000
tccaatgaac taaccccttc ccccaaccca ataaaacatt ggttttaatc ataataaaaa 30060
atcacttact ttaaatctga aacagtgtct ccgtccaagt tttcttgtag caccacttca 30120
ctcccctctt cccagctctg gtactgcaag ccccggtggg ctgcaaactt tctccacacc 30180
ttaaaaggga tgtcaaattc ctcttgtcca acaatcttca ttgtctcttc ctagatgtcc 30240
acaaagcgcg cgcgggtgga agatgacttt gaccctgtct acccatacga tgctgagctg 30300
gcaccgtctg tacccttcat cgcccctccc ttcgtttcgt cagacggatt tcaagaaaaa 30360
cccctgggag ttctgtccct aagactagcc aacccagtca ctactaaaaa tggggaactc 30420
acacttaaac tgggagatgg ggtgggcata gactcagatg gaaacctcac agcacagaca 30480
gttactaaag caacatcccc ccttactgtt tccaataacg caattgcact taacatggac 30540
aaaccttttt acagtagcaa tggaaaacta tccttacaag ttacatcacc attaaagata 30600
gtcgactctt taaatacatt ggctattggc tatgggcaag gcttaggact aaacaatagt 30660
gctcttgctg tgcaattagc atctcccctt acatttgaca gcaacagcaa aattaaaata 30720
aatttgggaa gcgggccatt aaaaattaat gcgaataaac tgtcaattaa ctgcctaagg 30780
ggtgtatatg taacaactga cggaacttcc attgaaacaa atataagctg ggcaaaagga 30840
atgaggtttg aaggtaatgc catggctgta aacgttgaca gcaccaaagg tctacaattt 30900
ggcactacca gcacagaatc aggagtcact aacgctttcc ctatccagtt aaagattgga 30960
tctggtctta gttttgacag cacaggagca cttgtagctt gggataagga taatgacaag 31020
cttacactgt ggacaaccgc tgacccatca cctaattgta ccatatatac agacaaggat 31080
gctaaactta cactttgtct tacaaaatgt ggcagtcaaa tactaggcag tgtttcagta 31140
ctggctgtta aagctggaac cctacagcca atcagtgaaa aaataggtac tgctttggtt 31200
tcactaaaat ttaataacaa cggtgtattg ttaagcaact ccacattaag taatgaatac 31260
tggaactaca ggaagggaga tgtcacacca gccgaagcct atactaatgc tgtgggtttt 31320
atgccaaaca tcaaggcata tcctaaaaac acaaactctg cctcaaaaag ccacattgta 31380
ggacaagtgt accttaatgg agatgaaact aaaccaatgc atttaatcat tacatttaat 31440
gaaaccagtg atgaaacatg cacatattcc ataacgttcc aatggaaatg gaacattgga 31500
acatacacca gcgacaccct tgcaacaagc tcctttacct tttcttacat tgcccaagaa 31560
taaaaactgc agacaacaat aaagtttaaa tgttttattt aaacagtttc acagaaccct 31620
agtattcaac ctgccacctc cctcccaaca cacagagtac acagtccttt ctccccggct 31680
ggccttaaaa agcatcatat catgggtaac agacatattc ttaggtgtta tattccacac 31740
ggtttcctgt cgagccaaac gctcatcagt gatattaata aactccccgg gcagctcact 31800
taagttcatg tcgctgtcca gctgctgagc cacaggctgc tgtccaactt gcggttgctt 31860
aacgggcggc gaaggagaag tccacgccta catgggggta gagtcataat cgtgcatcag 31920
gatagggcgg tggtgctgca gcagcgcgcg aataaactgc tgccgccgcc gctccgtcct 31980
gcaggaatac aacatggcag tggtctcctc agcgatgatt cgcaccgccc gcagcataag 32040
gcgccttgtc ctccgggcac agcagcgcac cctgatctca cttaaatcag cacagtaact 32100
gcagcacagc accacaatat tgttcaaaat cccacagtgc aaggcgctgt atccaaagct 32160
catggcgggg accacagaac ccacgtggcc atcataccac aagcgcaggt agattaagtg 32220
gcgacccctc ataaacacgc tggacataaa cattacctct tttggcatgt tgtaattcac 32280
cacctcccgg taccatataa acctctgatt aaacatggcg ccatccacca ccatcctaaa 32340
ccagctggcc aaaacctgcc cgccggctat acactgcagg gaaccgggac tggaacaatg 32400
acagtggaga gcccaggact cgtaaccatg gatcatcatg ctcgtcatga tatcaatgtt 32460
ggcacaacac aggcacacgt gcatacactt cctcaggatt acaagctcct cccgcgttag 32520
aaccatatcc cagggaacaa cccattcctg aatcagcgta aatcccacac tgcagggaag 32580
acctcgcacg taactcacgt tgtgcattgt caaagtgtta cattcgggca gcagcggatg 32640
atcctccagt atggtagcgc gggtttctgt ctcaaaagga ggtagacgat ccctactgta 32700
cggagtgcgc cgagacaacc gagatcgtgt tggtcgtagt gtcatgccaa atggaacgcc 32760
ggacgtagtc attctcgtac tttgagtggc aaaaccttgc tctcgaacag cacacgtctc 32820
gtcgcctcct gtcccttctc ttcgcctttt cagtgtgata gttgtaatac agccattcac 32880
gaagctcagt cagaagatct tcagcgtctg ttgtcaaaaa caatccatcc aatctgattg 32940
ctttcaaaac atcacaaaca gtcgaataag ccaaacccat ccaggcaatg caattatttt 33000
ggttatccac aatgggaggg ggcggaagac atggaagagg cataattaat tttttaatcc 33060
aatcgatcac gcagcacttc aaaatgaaga tcgcgaaggt gacacctttc acccccactg 33120
tgttgatgaa aaataacagc caagtcaaaa ttgatgcggt tttcaaggtg ctcgactgta 33180
gcatcaagca gagcttccac acgcacgtcc acaaataaca gaatagcaaa agcgggagga 33240
ggaagtaaat cctcaatcat catagtacag tccatcacca tccctaaata attttcatcc 33300
ttccagcctt ggactatatt tttaaactgc tcttgtaaat ccaaaccaca catgtggaaa 33360
agttcccaaa gagctccctc aactaccatt cttaagcaca ccttcatagt gacaaaatat 33420
cttgttcctc tgtcacctgc agcaaattac aaagtccaat attaggatct atgcccagag 33480
atctaagctc atccctcaat tccaactgta aaaaggcttc cagatctgcc ctaacttgtt 33540
cagccagtgg gctccctgga ataagcgtgg gagaagccaa actgcaaaac agacgcatgc 33600
cgccataatt accaccagaa aacactacgt tacagtatgc atgctgattc attccagtaa 33660
tttcatccag tgtattggat acaaaaaaag gcaagcactc tctcactaat tgtattatgg 33720
agacattatc acacaggtaa caatttaaag gttgtggaac aataatgcag taagtaacca 33780
cggtgcgctc caacatggtt agtaattttt agttctgaaa aacaaaacat acaaaaaatt 33840
atatcatact catttggcga actggtggaa aaatgaccct atctagcaca aggcaagcca 33900
ctggatcacc aatgcgcccc tcataaaacc tgtcatcatg attaaaaagc aacaccgaaa 33960
gctcttccct atgtcctgca tgaatgattc tagctgagga atataagcca gcgcaattag 34020
tatctgttaa agaaaaaaaa cggccaacat agcctctagg aattagcaca cttaatctta 34080
aagacattac tgccatcccc cttggattta aggtaaaatt tacaggagca tagaaaatat 34140
actgatttcc ctcctgcaca ggcagcatag caccaggtcc ctctaaaaac acacacaaag 34200
cttctgcagc catagcttac cgcgcaaacc aggcacagca gtgagctaaa aggacaaagc 34260
tctaactcac tagccaacct ggcgcacaat atatagttag tccttacact gacgtaaccg 34320
accaaagtct aaaaaccccg ccaaaaatac acacacgccc aaaaaacgcc ccgtgagtca 34380
aaaaacagtt tcacttcctc gttacaccca aaacgtcgtc acttccggat tcccacggtt 34440
cgtcacttcc ggagctcctt gcttaattaa ccccgcccaa aacgtcatcg tccgcgtcac 34500
gccgccccgc cccgcgaccg ttgaccccgg gccaatcacc gcacatcccg caaaattcaa 34560
actcgtctaa tttgcatatt ggcacactgc ccatataagg tatattattg atgatgattt 34620
aaatcatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 34680
ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 34740
atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 34800
gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 34860
gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 34920
gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 34980
gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 35040
aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 35100
ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 35160
taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 35220
tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 35280
gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 35340
taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 35400
tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 35460
tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 35520
ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 35580
taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 35640
tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 35700
cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 35760
gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 35820
cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 35880
ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc 35940
aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 36000
atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 36060
tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 36120
gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 36180
aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac 36240
acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 36300
ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 36360
tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 36420
aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 36480
catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 36540
atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 36600
aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 36660
gcgtatcacg aggccctttc gtcttcaaga attgatttaa at 36702
<210> 22
<211> 33625
<212> DNA
<213> 人工序列
<220>
<223> BLY6.FLuc腺病毒载体
<400> 22
catcatcaat aatatacctt atatgggcag tgtgccaata tgcaaattag acgagtttga 60
attttgcggg atgtgcggtg attggcccgg ggtcaacggt cgcggggcgg ggcggcgtga 120
cgcggacgat gacgttttgg ggaggaggag ctatgttgca agtaatcgtg ggaaatgcga 180
cgtaaaacga ggtggagttt aaacacggaa gtagacaatt ttcccgcgct gtttgacagg 240
aaatgatgtg tttttgggcg gatgcaagtg aaaattctcc attttcgcgc gaaaactaaa 300
tgaggaagtg aaattctgag taattctgag gttatcacag ggcggagtat ttaccgaggg 360
ccgagtagac tttgacccat tacgtggagg tttcgattac tctatttttc acctaaattt 420
ccgcgtactg tgtcaaagtc cgtgttttta cggcgatcgc tcaatattgg ccattagcca 480
tattattcat tggttatata gcataaatca atattggcta ttggccattg catacgttgt 540
atccatatca taatatgtac atttatattg gctcatgtcc aacattaccg ccatgttgac 600
attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 660
atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 720
acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 780
tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 840
tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 900
attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 960
tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 1020
ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 1080
accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 1140
gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga 1200
tcgcctggag acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca 1260
gcctccgcgg ccgggaacgg tgcattggaa gcttggcatt ccggtactgt tggtaaagcc 1320
accatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat 1380
ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca 1440
attgctttta cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg 1500
tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc 1560
gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga 1620
gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc 1680
atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg 1740
caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg attctaaaac ggattaccag 1800
ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac 1860
gattttgtgc cagagtcctt cgatagggac aagacaattg cactgatcat gaactcctct 1920
ggatctactg gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg cgtgagattc 1980
tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt 2040
gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga 2100
tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttctgaggag ccttcaggat 2160
tacaagattc aaagtgcgct gctggtgcca accctattct ccttcttcgc caaaagcact 2220
ctgattgaca aatacgattt atctaattta cacgaaattg cttctggtgg cgctcccctc 2280
tctaaggaag tcggggaagc ggttgccaag aggttccatc tgccaggtat caggcaagga 2340
tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg 2400
ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg 2460
aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc 2520
ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat 2580
tctggagaca tagcttactg ggacgaagac gaacacttct tcatcgttga ccgcctgaag 2640
tctctgatta agtacaaagg ctatcaggtg gctcccgctg aattggaatc catcttgctc 2700
caacacccca acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa 2760
cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg 2820
gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg 2880
gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc 2940
ataaaggcca agaagggcgg aaagatcgcc gtgtaattct agacgagatc cgaacttgtt 3000
tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 3060
atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 3120
ctgcgatcgc gatggcctgt gtttgagcat aatgtactga ccaggtgtaa cgttcatctg 3180
ggtggtcgta gaggaatgtt catgccatac caatgcaatt ttaatcatgt gaggatcttg 3240
atggagccgc aagcgttttc cagagtcagc ttgactggaa tctttgacat gtgtgtggaa 3300
gcatggaaga tcttaagata tgatgatacc aaatccagat gccgcgcatg cgagtgcggg 3360
ggcaggcatg ccaggttcca acctgtatgt gtggaggtga ccgaggagct gagaccagat 3420
catttggtgc tgacctgcac tggtgcggag ttcggttcca gtggtgaaga aactgattaa 3480
agtgagtagt gggatgttat aaaagtgacc ataaggtgat gtgagatgga caaatttggt 3540
aatttttatg tatttttgtc ttgcagccat gagtgggagc gcttcctttg aagggggcgt 3600
ctttagccct tatctgacgg ggcgtctgcc tcattgggct ggagtgcgtc agaatgtgat 3660
ggggtctaca gtggatggaa gacctgttca gcctgctaat tcttctactc tgacttatgc 3720
tactatgact tcctcgcctt tggatgcagc tgcagctgct gccgcttctg ctgccgccaa 3780
cactgttcgg gggatggcct tggagatggg gtattatgga actgtagtgg ccaacaccac 3840
taccccaaat aaccccacag ccttgaatga ggacaagctg ctagttctca tgtcccagct 3900
ggagtctttg acccaacgcc tgggcgatct agctcagcag gtgtcccagc tgaaggagca 3960
gactcaagct gcaattacca ctgcgagggg aaattaaaaa aattcaaaga atcaataaat 4020
aaaccgagac tttgttgatt ttaaagtgtg tcattcttta tttaattttt cgcgcgcgat 4080
atgccctgga ccaccggtct ctatcattga ggacacggtg gatcttttct agaacccgat 4140
agaggtggga ttggatgttg aggtacatgg gcataagacc atctttgggg tgtagatagc 4200
tccactgcag agcctcatgc tccggggtgg tgttgtatat aacccagtca tagcatgggc 4260
gttgggcatg atgttgcaca atatctttaa ggaggagact aatggccact gggagaccct 4320
tggtgtaagt gtttacaaat ctattaagct gggacgggtg catccgaggt gagataatgt 4380
gcattttgga ttggattttt agattggcaa tgtttccccc tagatctctc ctgggattca 4440
tgttatgcaa gaccactaga acagtgtatc cggtgcactt agggaatttg tcatgaagtt 4500
tggaggggaa agcatgaaaa aatttagaca cacccttgtg tcctcccaag ttctccatgc 4560
actcatccat aataatggca atgggcccat gggcggcggc acgggcgaac acgttcctgg 4620
gatctgacac atcatagttg tggtcttggg tcaggtcatc ataagccatt ttaataaact 4680
tggggcggag ggtgccagat tgggggatga atgttccctc gggccccgga acatagtttc 4740
cttcacatat ttgcatttcc caggctttta gttcagaggg ggggatcatg tccacctgtg 4800
gagcgatgaa gaagacggtc tcgggggcgg gggtgattaa gtgggaggac agcaagttcc 4860
taagcagctg tgacttgcca cacccagtgg gaccgtagat gacccctata acaggttgca 4920
gatggtagtt tagggaaaga cagctgccgt cctctcgcag gaggggggcg acctcgttca 4980
tcatttccct cacatgcatg ttttcccgca caagttccga taggaggcgc tctccaccca 5040
gggaaaggag ttcttgaaga gatgagaaat ttttcaaggg ttttaagcca tcagccatgg 5100
gcattttgga gagggtttgt tgcaagagtt caaggcggtc ccagagttcg gtgatgtgtt 5160
ctatggcatc tcgatccagc atacttcctc gtttctgggg ttgggacggc tgcgggagta 5220
tggaaccagg cgatgggcgt ccagcgctgc cagtgtccgg tccttccacg gtcgcagcgt 5280
ccgagtcagg gtcgtttccg tcacggtgaa ggggtgcgcg cctggctggg cgcttgcgag 5340
ggtgcgcttc aggctcatcc tgctcgtgga gaaccgctgc cgttctgcgc cctgtgcatc 5400
ggccaggtag caattaacca tgagttcgta gttgagcgcc tctgccgcgt ggcctttggc 5460
gcgcagctta cctttggaag tcttctgaca ggtgggacag tagagacact tgagagcata 5520
gagttttggg gctagaaaga ccgattctgg ggagtatgca tcggccccac aggaggcgca 5580
gacggtttcg cattccacca gccatgtaag atcgggctcg ttggggtcaa aaacaagttt 5640
tccgccatgt tttttgatgc gtttcttacc tttgctttcc atgagttcgt gcccccgttg 5700
ggtgacaaag aggctgtccg tgtccccgta gactgacttt atgggcctgt cctcgagcgg 5760
cgtgccgcgg tcctcttcgt agaggaactc ggaccactct gagacgaaag cacgtgtcca 5820
ggccagcaca aaggaggcta tatgggaggg gtagcgatcg ttgtcaacca aggggtctac 5880
tttttccaag gtgtgtaaac acatgtcccc ttcttccaca tccaggaagg tgattggctt 5940
gtaagtgtat gccacgtgac ctggggtccc agacgggggg gtataaaagg gggcgggtct 6000
ctgctcgtcc tcactgtctt ccggatcgct gtccaggagc gccagctgtt gaggtaggta 6060
ttccctctcg aaggcgggca taacctccgc actcaggttg tcagtttcta ggaacgagga 6120
ggatttgata ttgacagtgc ctgccgagat gcctttcatg agactgtcgt ccatttggtc 6180
agaaaagaca atctttttgt tatcaagttt ggtggcgaag gatccataca gggcattgga 6240
aagcagtttg gcaatggagc gcatggtttg gtttttttct ttgtctgcgc gctctttggc 6300
ggctatgttg agttggacat attcgcgggc cagacatttc cattgtggaa atatggtagt 6360
taattcatct gggacgattc tgactttcca gcctctgtta tgcagggtaa tcagatccac 6420
actggttgcc acttctcctc taagtggttc attagtccag catagtcgcc ccccttttcg 6480
agaacagaaa gggggtaggg gatctagcat gagttcgtct ggggggtctg catctatggt 6540
gaaaatccca ggaaggagat cttcgtcaaa atagctgatg gtggcggggt catccagaga 6600
catttgccat tctcgagcag ccagagcgcg ctcgtagggg ttaaggggag tcccccatgg 6660
catgggatgg gtgagtgcag aagcatacat gccacagatg tcatagacat agagcggctc 6720
ttccagaatc cctatgtaag tgggataaca tcgcccccct ctgatgctgg ctcgcacata 6780
atcatagagt tcatgtgagg gcgctagaag acccgagccc aggttggtgc ggttgggttt 6840
ttctgctctg tagaggatct ggcgaaagat ggcatgggag tttgatgaga tggtgggtct 6900
ttggaagatg ttgaaatggg catgaggcag tcccacagag tcccttatga agtgagcata 6960
ggagtcttgc agtttggcca ccagctcggc ggtgaccagc acatccaaag cacagtagtc 7020
gagggtctct ttgatgatgt catagttagg ttcccctttc ttttcccaca gctcgcggtt 7080
gagaaggtat tcttcgcgat ccttccagta ctcttcgagg gggaacccgt ccttgtctga 7140
acggtaagaa cccagcatgt aaaattgatt gacagctttg taggcacaac accccttctc 7200
cacggggagt gagtatgctt gcgcggcttt gcgcagagag gtgtgagtaa gggcgaaagt 7260
gtccctgacc atgactttga ggaactgatg cttaaagtct atgtcatcgc aggccccctg 7320
ctcccacagt tggaagtcca ctcgcttttt gtaggcggga ttgggcaaag cgaaagtaac 7380
atcgttgaat aggatctttc cagccctggg catgaagttg cgagtaatgc gaaaaggctg 7440
aggcacttct gccctgttgt tgataacttg ggcagccaag acgatctcgt caaagccgtt 7500
gatgttgtga cccacaatgt aaagttctac gaagcgtggg cgtcccttga tgtggggcag 7560
ttttttaagc tcttcgtagg tcaagtcgtc agggtcagcg attccatatt gctccaaagc 7620
ccagtcaggc aggtgaggat tagcatgaag gaaagaggtc caaagatcca cggccagagc 7680
tgtttgtaag cggtctctgt actgacggaa atgtcggcct accgccattt tttcaggagt 7740
aacacagtaa aaggtgcgcg ggtccttttc ccagcgatcc cattgaagtt gcaaggctag 7800
gtcgtgggcg aggttgacga gctgttcgtc ccccgaaagt ttcatgacca gcatgaaagg 7860
gacaagctgc ttgccaaagg accccatcca ggtgtaggtt tccacatcgt aggtgaggaa 7920
gagcctttct gtgcgaggat gagaaccgat cgggaagaac tggatttcct gccaccagtt 7980
ggaggaatgg ctgttgatat gatggaagta gaactcccta cggcgcgccg agcattcgtg 8040
cttgtgcttg tacagacggc cacagtactc gcagcgctgc acgggatgca cctgatgaat 8100
gagctgtacc tggcttcctt tgacaagaaa tttcagtggg aagttgaggc gtggcgtctg 8160
catctcgtgt tgtattacgt cctggctatt ggtctggcca tcttctgtct cgatggtggt 8220
catgctgacg agcccgcgcg ggaggcaggt ccagacctcc gcgcggacgg gtctgagagc 8280
gaggacgaga gcgcgcaggc cggaactgtc cagggtcctg agacgctgcg gagtcaggtc 8340
agtagggaga gtacataggt ttacttgcat aagtttttcc agggcatgtg ggaggtcaag 8400
atgatatttg atttctactg gcgagttggt ggagacatcg atggcttgca gggtcccgtg 8460
cccctggggt gctaccaccg tccctttttt tttcttgatc gggggcggtg ttgcttcttg 8520
catggtaagg tcgtcttcta gaagcggcgg cgaggtcgcg cgccgggtgg cagtggcggt 8580
tctggacctg gaggtagagg cggtagaggt acgtcggcgc cgcgcgcggg taggttctgg 8640
tactgcgccc tgagaagact tgcgtgagcg acgacgcggc ggttgacgtc ctggatctga 8700
cgcctctggg tgaatgctac cggacccgtg agcttgaacc tgaaagagag ttcaacagaa 8760
tcaatttcgg tatcgttgac ggctgcctgc cgcaggattt cttgtacgtc gcccgagttg 8820
tcttggtagg cgatctcggc catgaactgc tcgatctctt cttcttggag atctccgcgg 8880
cccgctcgtt ctacggtggc agcaaggtcg ttggagatgc gccccatgag ctgtgagaat 8940
gcattcatgc ccgcctcgtt ccagacgcga ctgtagacca cggctccctc gggatctctg 9000
gcgcgcatga ccacttgggc gaggtttagt tccacgtgtc tggtgaagac cgcatagttg 9060
cagagacgct ggaagaggta gttgagcgtg gtggcgatgt gctcggtgac aaagaaatac 9120
atgatccagc gacgaagcgg catctcgctg atatcgccca gggcttccaa ccgttccatg 9180
gcttcgtaaa agtccacggc gaagttgaaa aactgggagt tgcgagcgga cacggtcaac 9240
tcctcctcca gaagacggat gagctcggcg atggtggcgc gcacttcgcg ctcaaaggct 9300
cccgggatct cttcctcctc ttcttcttcc aactcttcct ccactaacat ctcttctact 9360
tcctcctcag gcggcggggg tggaggaggg ggcgcgcggc gacgccggcg acgcacgggc 9420
agacgatcga tgaagcgttc gatcacttct ccgcggcggc gacgcatggt ctcggtgacg 9480
gcgcgcccgt cctccctggg tcgcagagtg aagacgccgc cgcgcagctc cctgaaatgg 9540
tgactgggag ggtccccgtt tggtagggac agggcactga tgatgcatct tattaattgc 9600
cctgtaggga ctccgcgcaa ggacctgagc gtctcgagat ccacgggatc tgaaaatctc 9660
tgaacgaagg cttcgagcca gtcgcagtcg caaggtaggc tgagcactgt ttcttcgggg 9720
cgggctgctg agctagaggg ttgtacgatg ctgctggtga tgaagttaaa ataggcagtt 9780
ctgagacggc ggatggtggc gaggagcacc aggtctttgg gtccggcttg ctggatgcgc 9840
aggcggtcgg ccattcccca tgcattatct tggcacctgg ccagatcttt atagtagtct 9900
tgcatgagtc gctccacggg cacttcttct tcgcccgctc tgccgtgcat gcgtgtgagt 9960
ccgtaccctc tctgtggttg gacgagcgcc aggtcggcaa cgaccctttc ggctagaatg 10020
gcttgctgca cctgggtgag ggtgttctgg aaatcatcaa agtccacaaa gcggtggtag 10080
gcccccgtgt tgatggtgta ggagcagttg gacatgaccg accagttgac tgtctggtgt 10140
cctggtcgta cgagttccgt gtacctgagc cgcgagtatg cgcgggagtc gaagatgtaa 10200
tcgttgcagg ttcgcaccag gtactggtag ccgatgagga agtgaggcgg cggctggcgg 10260
tagagaggcc atcgttcggt ggcgggcgcg ccgggcgcta ggtcttctag catgagacgg 10320
tggtatccgt agacgtacct ggacatccag gtaataccgg cggcggtggt ggaggcgcgc 10380
ggaaactctc gcacgcggtt ccagatgttg cgcagcggca tgaagtagtt catggtgggc 10440
acggtctggc ccgtgaggcg cgcgcagtca ttgatgctct agatacgggc aaaaacgaaa 10500
gcgttgagcg gttcccttcc gtggcctgga ggaacgcgaa cgggttaggt cgcagcgtac 10560
cctggttcga gactaaagaa agcgagcaac tcgaaccggc agagtcgcgg ctaacgggta 10620
ttggcaatcc cgtctcgacc caagccagca aatccaggat acggatgggg gccccttttg 10680
tttttcaggg catgagtcac cggttaaggt ttacaacggc tgtttcatgc ctttagaagt 10740
ggctcgcgcc cgtagtctgg agaatcaatc gccagggttg cgttgcggcg tgccccggtt 10800
cgagcctgca gcttgagtcg gccggtgacc gcggcaaacg agggcgtggc ggccccgtcg 10860
tttctaagac cttgctagcc gacctctcca gtttacggga acgagccccc ttttattttt 10920
tttgtttttg ccagatgcat cccgtactgc ggcagatgcg cccacagccc ccacagcagc 10980
agcagcaggc tggcctacct tctctacctc agccgctacc tgcaactacc gcggtggccg 11040
ctgtaagcgg ggccggacag caggcggctc ctcaatatga attggacttg gaagagggcg 11100
agggattggc aagattgggg gcgccctcgc ccgagcgcca cccgcgggtg cagatgaaaa 11160
aggacgttcg cgaatcttac gtgcccaagc agaatctgtt cagagacaga agcggcgagg 11220
agcccgagga gatgcgcgcg tcccgtttta acgcgggtcg cgagctgcga caaggactgg 11280
atcgaaaacg ggtgttgagg gatgatgatt ttgaggtgga tgaaatgaca gggatcagcc 11340
ccgctcgcgc tcacgtggct gcagctaatc tggtgacagc ttatgagcag accgtgaagg 11400
aggaaagcaa cttccagaaa tcattcaata accacgtgcg caccctgatc gcacgcgagg 11460
aggtgaccct gggcctgatg cacctgtggg atctgctgga agccatagtg cagaacccca 11520
ctagcaaacc cctgactgct caactgtttc tggtggtgca gcacagcagg gataatgagg 11580
cattcagaga ggcgctgctg aatatcactg aacctgaggg gagatggctg ctggatctgg 11640
tgaatatcct gcagagcatt gtagtgcagg aacgcagctt gcctttgtcc gagaaggtgg 11700
cggcgatcaa ttactctgtg ctgagtctgg gcaaatacta tgccaggaag atctacaaaa 11760
ccccttacgt gcccatagac aaggaagtga aaatagatgg gttttacatg cgcatgaccc 11820
tgaaagtgct aaccctgagc gatgacttgg gagtgtaccg caacgacagg atgcaccgcg 11880
cggtgagcgc cagcaggagg cgcgagctga gcgacaaaga attaatgcac agcttgcaac 11940
gagccctgac gggagccggg acggaggggg agaactactt tgacatgggt gcagacttgc 12000
attggctgcc tagtcgcagg gcattggaag cggcaggcga tgggccctat gtagaggaag 12060
tagtagacga ggacgatgag gagggcgagt acctggaaga ctgatggcgc gacccgtatt 12120
tttgctagat ggaacaggcg ccggaccctg cgatgcgggc ggcgctgcag agccagccgt 12180
ccggcattaa ttcctcggac gattggaccc aggccatgca acgcatcatg gcgctgacga 12240
cccgcaaccc cgaagccttt agacagcagc ctcaggccaa ccgcctttcg gccatcctgg 12300
aggccgtggt gccctctcgc tccaacccca cccacgagaa ggttctggcc atcgtgaatg 12360
ccctggtgga gaacaaggcc atccgctccg atgaagccgg gctggtatac aacgccttgc 12420
tcgagcgcgt ggctcgctac aacagcagca atgtccagac taacctggac aggatggtga 12480
ccgacgtgcg cgaggccgtg tcccagcgcg aacggttcca tcgcgagtct aacctgggtt 12540
ccatggtagc gctgaacgct ttcctcagtt cccagcctgc caatgtgccc cggggacagg 12600
aagactatac caactttatt agcgccctga gactcatggt agccgaggtt cctcagagcg 12660
aggtgtacca gtccggtcca gactactttt tccagacaag caggaacggt atgcagacag 12720
tgaacttaag ccaggctttc aagaacctgc aagggctgtg gggagtccaa gctccagtgg 12780
gcgacagggc gaccgtgtcg agcctgttga ctccaaattc ccgtttgctg ctgctgctgg 12840
tgtccccctt cactgacagc ggcagcataa acagaaactc ctacttgggc tacctgataa 12900
acttgtatcg cgaagctata ggtcaggccc acgtggacga acagacctat caggagatca 12960
ctaatgtgag tcgcgctctg ggccaggacg accctggaaa cctggaagct actctaaact 13020
ttctgctgac caaccgctcg caaaaaatcc ctcctcagta tacattaact gcggaggagg 13080
aacggatctt gagatacgtg cagcagagcg tgggtctgtt cctgatgcaa gagggtgcga 13140
cccctagcgc cgcgcttgat atgacagcgc gcaacatgga gcccagcatg tatgccagca 13200
acagaccatt cattaataaa ttgatggatt acttccatcg cgcggccgct atgaactctg 13260
attacttcac caatgctatt ctgaaccccc attggctgcc tccgcctggt ttttatactg 13320
gcgagtatga catgcctgac cccaacgatg ggttcttgtg ggacgatgtg gacagcgtgg 13380
cgttctcgcc taccgctcct cgtacttttt ggaagaagga aggtagtgac agaagaccct 13440
cctccgtgct gtcaggacgt gagggtgctg ccgcggcggt ccccgatgct gcaagcccct 13500
ttcccagtct gccattttca ctaaacagcg tgcgcagtag cgagctgggg agaataaccc 13560
gccctcgctt gctgggcgag gacgagtatt tgaatgactc cctactgaga cccgagcggg 13620
aaaagaactt ccctaataat gggattgaaa gcctggtgga taagatgagc agatggaaga 13680
cctatgccca ggagcacaga gatgagccta gaatcttggg tcctacagta ggcacccgca 13740
gacgccagcg ccatgataga cagcggggtc tggtgtggga cgatgaggat tctgcagatg 13800
acagcagcgt gttggacttg ggcgggaggg gaggtgtggg caacccgttc gcacacttgc 13860
gtccccgtat tggacgcatg atgtaaaagt gaaaataaaa aaggaactca ccaaggccat 13920
ggcgaccagc gtgcgttcgt tctttctgtt gttgtatcta gtatgatgag gcgcaccgtg 13980
ctaggcggat cggtggcgta tccggagggt cctcctcctt cgtacgaaag cgtgatgcag 14040
caggtggcgg cggcggcgat gcaacccccc ttggaggctc cttacgtgcc cccgcggtac 14100
ctggcaccta ccgaggggag aaacagcatt cgttattcgg aactcacacc cttgtatgac 14160
accacccggt tgtacctggt ggacaacaaa tcggcggaca ttgcctcgtt gaactatcag 14220
aacgaccaca gcaacttctt gacaacggtg gtgcagaaca atgactttac ccccacggag 14280
gccagcaccc agaccatcaa ctttgacgag cgctcccggt ggggcggtca gctgaagacc 14340
atcatgcaca ccaacatgcc caacgtgaac gagttcatgt ttagcaacaa gttcagggct 14400
agggtgatgg tgtccagaac cacacctaaa gaggtgacag tcacaacaga ctatgatggt 14460
agtcaggaca tcttggaata cgagtgggtt gactttgagt taccagaagg caacttctct 14520
gccaccatga ccatagacct gatgaataat gcaattgttg ataattacct aaaagtgggt 14580
agacagaatg gggtactgga gagtgacata ggtgttaagt ttgacactag gaactttagg 14640
cttggttggg acccagtgac agagttggtc atgcctgggg tctacaccaa tgaagctttc 14700
catcctgaca tagtcctact acctggctgc ggagtggact tcactgagag ccgcctcagt 14760
aatctgctag gcattagaaa gaaacagcca ttccaggaag ggttccagat catgtatgag 14820
gatctggagg gtggtaacat ccccgccctg cttgatgtaa atgcatatga gaagagcaag 14880
gaagataata caaccaccac aaatgaagct gtggccgcgg cttcatctac tgaagccaaa 14940
gctgtggtag atgcttccac ttcaacagaa aacaccactg atgaaaaagt caccagggga 15000
gatacatttg ccacccctga acaagagaag gcagctgagg cagagtctga tattatgctt 15060
ctgtccaccg atgaaaacga aactaaaaaa caactggtta ttcgagcggt gaccaaggat 15120
agtaaggaca ggagttataa tgtattgtca gatggaaaga acacagctta ccgtagttgg 15180
tacctggcat acaattatgg cgaccgtgag aaaggggtgc gttcttggac actgcttacc 15240
acctcggatg tcacctgcgg cgtggagcaa gtctattggt cgctaccaga tatgatgcaa 15300
gatccagtca cctttcgctc cacacgccaa gttagcaact acccagtggt gggcgcagag 15360
ctgctcccag tgcattccag aagcttctac aacgagcaag ccgtctactc gcaacagctc 15420
cgccagtaca cctcgctcac gcacgtcttc aaccgcttcc ccgagaatca gatcctcgtc 15480
cgcccgcccg cgccaaccat taccaccgtc agtgaaaacg ttcctgctct cacagatcac 15540
gggaccctgc cgctgcgcag cagtatccgg ggagtccagc gcgtgaccgt tactgacgcc 15600
agacgccgca cctgtccata cgtatacaag gccctgggca tagtcgcgcc gcgcgtcctt 15660
tcaagccgca ctttctaaaa aaatgtccat tctcatctcg cccagtaata acaccggttg 15720
gggcctgcgc acacctagca agatgtatgg aggcgctcgc agacgctcca ctcagcaccc 15780
tgtgcgcgtg cgcgggcatt tccgcgctcc ctggggcgcc ctcaagggac gctctcgtac 15840
taggaccacc gttgacgatg tgatcgacca ggtggtcgcc gatgcacgta actatacccc 15900
cgcagccgca cctgcatcca ccgtggatgc ggtcattgac agcgtggtag ccgatgcgcg 15960
cgcctatgct cgcgccaaga gcaggaggcg gcgtattgcc aggcgtcacc gagctactcc 16020
agccatgcga gctgcaagag ctttattgcg gagagccaga cgtgtggggc gaagagccat 16080
gcgtagagcg gccagacgcg cggcttcagg tgccagcgca ggcagggtcc gcaggcgcgc 16140
ggctacggcg gcagcggcgg ccatcgctag catgaccaaa ccacgaagag gcaatgtgta 16200
ttgggtgcgc gacgccgcca ccggccagcg cgtgcccgtg cgcacacgcc cccctcgcac 16260
ttagaagata ctgagcagtc tccgatgttg tgtcccagcg gcgagatgtc caagcgcaaa 16320
ttcaaggaag agatgctcca ggtcatcgcg cctgagatct acggtcctgc ggtgaaggat 16380
gaaaaaaagc cccgcaagat caagcgggtc aaaaaggaca aaaaggaaga agatggtgat 16440
gatgggctgg tggagtttgt gcgcgagttt gccccaagga ggcgcgtgca gtggcgcggg 16500
cgcaaagtgt ggccggtgtt gagaccgggg accacagtgg tctttacgcc aggcgagcgc 16560
tccagcaccg tttccaaacg ctcttatgat gaggtgtacg gggacgatga tattctcgag 16620
caggcggctg atcgccttgg cgagtttgca tatggcaaac gcagccgctc gggagccaag 16680
gaagaggcat tgaccatccc cttggatcat ggaaatccca ccccaagcct caaacccgtg 16740
accctgcaac aagtgctgcc cacgccgcca cgcaagggca tcaagcgcga gggcgaggat 16800
ctgtatccca ccatgcagct gatggtgccc aagcgccaga agctggaaga cgtgctggag 16860
aaaatgaaag tggatcctga aatccagcct gaagtcaaag tgaggccaat caagcaggtg 16920
gcgcccggtt tgggggtaca aaccgtggat atcaagatcc ccaccgagtc catggaaatt 16980
caaaccgaac ccatgaagcc cacctccagc accattgagg tgcagacgga tccttggatg 17040
cccgcgcctg ctcctgttac cactactact cgaagaccta gaagaaagta tggttcagcc 17100
aacctgataa tgccaaacta tgctctgcat ccatcaatca tacccactcc tggctaccgc 17160
ggcactcgct actaccgcag tcacagcacc cgccgacgta aagcacctgc cacccgccgc 17220
cgtcgccgcc gccgtgccac tagcaaactt accccctcgg ctatggtgcg gagagtgtac 17280
cgtgatgggc gcgcagctcc tctgacactg ccgcgcgcgc gctaccatcc tagcattgcc 17340
atttaacaac tctgcctcct tgcagatatg gccctcactt gccgccttcg tattcctatt 17400
gctggctacc gcggaagaaa gtcgcgccgt agaagagcag ggttgtctgg gagcgggatg 17460
cgtcgccacc ggcggcggcg cgccatcagc aaacggttgg ggggtggatt tcttcccgct 17520
ttgattccca tcatcgccgc ggcgatcggc gcgataccag gcatagcttc cgtggcggtg 17580
caggcctcgc agcgccactg acattggaaa aagatatctt ataaataaaa atagaatgga 17640
ctctgacgct cctggtcctg tgatatgttt ttgtagacga gatggaagac atcaattttt 17700
catccctggc tccgcgacac ggcacgcggc cgtatatggg cacctggagc gacatcggca 17760
acagccaact gaacggggga gccttcaatt ggagcagtct atggagcggg cttaaaaatt 17820
ttgggtccac tataaagact tatgggaaca aagcttggaa cagcagcaca gggcatgcgc 17880
tgagacaaaa gcttaaagat cagaatttcc aacagaaggt ggtcgatggt atcgcctctg 17940
gaatcaatgg ggtggtagat ctggccaacc aggccgtgca gaaacagatt aacagtcgcc 18000
tggacccggc tcccccagct cctattcatg agttaatgca agtggaggaa gagctccctt 18060
cattggaaaa gcggggcgat aagcgacctc gtccagatat ggaggaaacg ctgctgacca 18120
aggtggatga gccgccctcc tatgaagagg ctgtaaaact gggaatgccc actacaaagc 18180
ccattatgcc tctggccact ggagtgatga agccatctca gtctaaacct gcagttgctg 18240
ctacattgga cttgcccgct cccgtggcca cccccaaacc tgtcgccgcc ccgaagccca 18300
ccgccgtgca acccgtggcc gtggccagac cgcgtcccgg tggtcggccg aatgcaaact 18360
ggcagagcac tctgaacagc atcgtgggtt tgggagtgca cagtgtgaag cgccgtcgct 18420
gctattgatt aaatatggag tagcgcttaa cttgcttgtc tgtgtgtgta tatgtcgatg 18480
ccgcccgccg tgctacagca aagagagaag gagaagaggc gccgctgagt tcctttcaag 18540
atggccaccc catcgatgct gccccagtgg gcgtacatgc acatcgccgg acaggacgct 18600
tcggagtacc tgagtccggg tctggtgcag ttcgcccgcg ccacagatac ctacttcaat 18660
ctggggaaca agtttaggaa ccctaccgtg gctcccaccc acgatgtgac caccgaccgt 18720
agccagcgcc tgacgctgcg ctttgtgccc gttgaccggg aggacaatac ctactcctac 18780
aaagtcagat acaccctggc tgtgggagac aacagggtgt tggatatggc cagcacctac 18840
tttgacatca ggggcgtgtt ggacagagga cctagcttca aaccatactc tggcactgcc 18900
tacaactccc tggctccaaa aggagctcca aactccagtc agtggcaaca aaaggaaaac 18960
aatggtcaag gtgatgcaaa gactcacacc tatggtgtag ctgccactgg aggtattgac 19020
attgacaaaa atggtcttca aattggaatc gatgaaacta aagaagataa taacgaaatt 19080
tatgcagaca aaacattcca acctgaacct caaattggag aagaaaactg gcaagatagc 19140
gaaaactatt atggaggcag ggctcttaaa ccggaaacca agatgaagcc ttgctatggt 19200
tccttcgcta gaccaactaa tgcaaaggga ggtcaagcca aaattaaacc agctcaagag 19260
ggtcaacagt ctatagatta tgacatagac ctggctttct ttgatattcc aagcactggc 19320
ggaggcaatg gcacaaatgt aaatgacaag ccagatatgg ttatgtatac tgaaaatgta 19380
aatctggaaa ctccagacac tcatcttgtt tacaagccag gaacttcaga tgacagttcc 19440
gaggccaatt taactcagca agccatggct aacagaccca actatattgg gtttagagat 19500
aactttattg gcgtcatgta ctacaacagc actggcaaca tgggagtgct tgctggtcaa 19560
gcatcccagc taaatgctgt ggtggacctg caagacagaa acaccgagct gtcttatcag 19620
ctattacttg actctctggg cgacagaacc aggtatttta gtatgtggaa tcaggcggtg 19680
gacagctatg atcctgatgt gcgcattatt gaaaaccatg gtgtggaaga tgaattgcca 19740
aactattgct tcccattgga cggagctggc actaatgctg tttaccaagg agttaagaca 19800
aaagaggata ataatggcga atgggaaaca gacacaaatg ttgcatcgca gaatcagata 19860
tgcaagggca acatatatgc tatggagatc aacctgcaag ccaacctgtg gaaaagtttc 19920
ctttactcca acgtggctct gtacctacca gactcctaca agtacactcc atccaacgtg 19980
acactcccta ccaacactaa cacctatgac tacatgaatg gcagggtggt gtctccatcc 20040
ctggtggatg cctacattaa cattggcgcc aggtggtctc tggatgccat ggacaatgtc 20100
aaccctttca accaccaccg caatgccggc ctgcgctacc ggtccatgct tctgggcaac 20160
ggccgatacg tgcccttcca catccaagtg ccccagaaat tcttcgctat caagaacctg 20220
ctgcttctcc caggctcata cacctacgag tggaacttcc gcaaggatgt caacatgatc 20280
ctgcagagtt cccttggcaa tgacctcaga accgatgggg ccaccatcca gtacaccagc 20340
atcaatctct atgccacctt cttccccatg gctcacaaca ctgcctccac cctggaagcc 20400
atgctgcgca atgacaccaa tgaccagtcc ttcaatgact acctctcagc tgccaacatg 20460
ctttacccca tccctgccaa tgccaccaac gtgcccatct ccatcccatc tcgtaactgg 20520
gctgccttca ggggctggtc tttcacccgc ctcaagacca aggagacccc atctctggga 20580
tcagggttcg atccctactt cgtctactca ggctccattc catacctgga tggaactttc 20640
taccttaacc acactttcaa gaaagtctcc atcatgtttg actcttctgt cagctggcca 20700
ggcaatgaca ggctgctgac tcccaatgag ttcgaaatca agcgcactgt tgatggggaa 20760
gggtacaatg tggcacaatg caacatgacc aaagactggt tcctggttca gatgctctcc 20820
cactacaaca ttggctacca gggcttctac atcccagaag gatacaagga ccgcatgtac 20880
tccttcttca gaaacttcca gcccatgagc cgccaggtgg tcgatcaggt caactacaaa 20940
gactacatgg cagtcaccct tgcctatcag cacaacaact ctggctttgt gggctacctc 21000
gcgcccacca tgcgacaggg ccaaccctac cctgctaact acccataccc gctcattggc 21060
aagactgcag tcaacagtgt cacccagaaa aagttcctct gcgacagggt catgtggcgc 21120
atccccttct ccagcaactt catgtccatg ggggccctta ccgacctggg gcaaaacatg 21180
ctttatgcca actccgccca cgcgctagac atgaatttcg aagtagaccc catggatgag 21240
tccacccttc tctatgttgt cttcgaagtc ttcgacgtgg tcagagtgca ccagccccac 21300
cgcggcgtca tcgaagctgt ctacctgcgc acccccttct cagctggtaa cgccaccaca 21360
taagcgcctt gcttcttgca agtggctgca gcagcatggc ctgtggatcc tccactggat 21420
ccaatgagca agagctcagg gccatcgcca tagacctggg ctgtggaccc tatttcctgg 21480
gaacctttga caagcggttt ccaggcttca tggctcctga caagctcgcc tgtgccattg 21540
tcaacacggc agggcgcgag actggtggtg agcactggct ggcttttgga tggaaccccc 21600
gctccaatac ctgctatctc tttgacccgt ttgggttttc agacgagcgc ctcaagcaga 21660
tctatcaatt cgagtacgag gggctcctgc gccgcagtgc cctggctact aaggaccgat 21720
gcatcactct ggaaaagtct acccagaccg tgcagggtcc gcgctcggct gcctgcgggc 21780
tcttctgctg catgttcctc catgcttttg tgcactggcc cgaccgcccc atggacaaca 21840
accccaccat gaatttgctg acgggggtac ccaacaacat gctccaatcg ccccaagtag 21900
agcccaccct gcgccacaac caggaggcac tctatcgctt cctgaactcc cactcatctt 21960
actttcgttc taaccgcgcg cgcattgaga aggccactgc cttcgatcga atgaataata 22020
acatgtaaac caaattgtgt gtggctcaaa taaacagcac tttattgttt acatgcactg 22080
aggctctggg atgatcattt tttaaaaatc gaaggggttc tggcgggaat cagcatggcc 22140
agatggcagg gacacgttgc ggaactggaa cttgttctgc cacttgaact cgggaatcac 22200
cagcctggga actggaatct ctggaaaggt atcttgccat agctttctgg tcagttgcag 22260
agcgccaagc aggtcaggag cagatatctt gaaatcacag ttggggccag aattctgggc 22320
gcgggagttg cggtacactg ggttgcagca ctggaacacc ataagggcag ggtgtctcac 22380
gctcgccagc acggtctcgt cactgatgca agacacatcc aggtcttcag cattggccat 22440
tccaaagggg gtcatcttgc aggtctgtct gcccatcacg ggagcgcagc caggtttgtg 22500
gttgcaatca caatgaaggg ggatcagcat catcttggcc tggtcggggg taatccctgg 22560
gtaaacagcc ttcatgaagg cttcatactg cttgaaagct tcctgggctt tggttccctc 22620
ggtgtagaac actccacaag acttgctgga aaactgatta gtagcgcagt tggcatcatt 22680
cacacagcag cgggcgtcgt tattagccag ctggaccaca ttcctgcccc agcggttctg 22740
ggtgatcttg gctcgatctg ggttctcctt caacgcgcgc tggccgttct cgctcgccac 22800
atccatctca atgacatgtt ccttctggat catgatgttg ccatgcaggc atctaatctt 22860
gccttcataa tcagtgcagc catgaggcca cagcgcgcac ccggtgcact cccaattgtt 22920
atgggggatc tgggaatggc tatgaaccag cccttgcagg aatcttccca tcatcacagc 22980
cagggtcttt atgctggtaa aggtcagcgg gataccgcgg tgctcctcgt tcacatactg 23040
ctggcagatg cgtctgtagt gctcggcctg ctcgggcatc agcttgaaag aggttttcaa 23100
ctcattatcc agcctgtatc tctccatcat gatggacatt acttccatgc ccttctccca 23160
ggcagaaaca atagggagac tcaggggatt cttgacagta gagacaacct tacttaaggg 23220
gtcatcactg ccaatctttt cgatgcttct cttgccatcc ttctcggtga tgcgcaccgg 23280
cgggtagctg aatcccacag ccaccaactg agcctcttcc ctttcgtctt cgctgtcttg 23340
actgatgtct tgcagaggaa catgtttggt tttcctgggt ttcttcttgg gcggcagctc 23400
tggaggactc tggctccgtt ccggagaccc catggatgag cgagagttgt cgctcaccac 23460
ttggatctgg ctgcctgtag aagaactgga ccccacgcgg cggtaggtgt tcctcttggt 23520
aggcagaggt ggaggcgacg ggctccggtc cggtctgggt ggcggatggc tggcggagcc 23580
ccttccgcgt tcgggggtgc gctccagatg gcggtcgtct gactgacctc cgcggctggc 23640
cattgtgttc tcctaggtag agaaacaaga catggagact cagccatcgc tgccatcgcc 23700
atccaccacc acaagcaccg ccgaggagga ggagtgttta accaccccac catgcagccc 23760
cgctaccacc accagcaccc ttgaaagcga ggtcgacacg gtcgtggagg atttacaggc 23820
tatggaagat attgaggcag ctgtcgagca agaccccggc tatgtgacac cggcggagca 23880
tgatgaggat ctagcgcgct ttctcgacgg tgtggagaaa gcgaaacaag atgaggacga 23940
ggaagaggca gaagcacaac catcggtggc cgactacctc accggcctag ggctagaaga 24000
cgtgctgctt aagcatcttg caaggcagac agtcatagtc aaagacgccc tgctagagcg 24060
ctccgaggtg ccactcagtg tggaagacct cagtcgcgcc tatgagctaa acctcttctc 24120
gcctcgcaag ccccccaagc gtcagcccaa cgggacctgt gagcccaatc cgcgcctcaa 24180
cttctatcca gccttcactg tgcccgaagt actagctacc taccacatct ttttcaagaa 24240
ccaaaagatc cccatctcct gccgcgccaa tcgcacccgc gcagatgccc tactcaactt 24300
ggggcccggc gctcgcatac ctgatatcgc ttccttggaa gaggttccta agatctttga 24360
gggtctgggc aatgaggaaa ctcgggcagc aaacgctctg caaagagaaa cagatgatgg 24420
tgaacaccac agcgctctgg tggagctcca gggcgacaac gctcgtcttg cagtcctcaa 24480
acgcagcatc gaggtcaccc atttcgccta ccccgcactt aatctcccac ccaaagtcat 24540
gagctcggtc atggacacgt tgctcatgaa gcgcgcgagc cccatctccg aggatcagaa 24600
catgcaggac cccgatgcct cagatgaagg caagcctgta gtcagcgacg agcaactggc 24660
tcgctggcta ggctctgact ccccccagtc tttggaggag cggcgcaagc ttatgatggc 24720
agtggtcctg atcacagcgg agctggagtg tctccgccgc ttcttcactg acccagagac 24780
cctgcgcaag cttgaggaga acctgcatta cacattcagt catgggttcg tgcgccaggc 24840
gtgcaagatc tccaacgttc aactcaccaa cctggtctcc tacctgggca tcttgcatga 24900
aaaccggctg gggcagaacg tgctccacac caccctgaag ggggaggccc gccgcgacta 24960
tatccgcgac tgtatctacc tctacctatg ctacacctgg caaagcggga tgggtgtgtg 25020
gcaacagtgc ttggaagagc aaaatctaaa agagctggaa aagctgcttc agaaatctct 25080
taaatctctg tggaccgggt tcgatgagcg gaccaccgct tcggacatgg ccgatattat 25140
cttccccgag cggctcagac acactctgcg cgacgggctg cctgactttg ccagccagag 25200
catgctacaa aactttaggt cattcatctt ggaacgctcc gggatcctgc ccgccacttg 25260
ctgcgcactg ccctccgatt ttgtgcccat cacctaccgg gagtgccccc cgccgctatg 25320
gagccactgc tacctgttcc gcctggccaa ctacttggcc taccactctg atgtgataga 25380
agatgttagt ggcgaagggc tcctggagtg ccactgccgc tgcaacctct gcacccccca 25440
ccgctccctc gcctgcaatc cccagctgct gagcgaaacc cagatcatcg gcaccttcga 25500
gttgcaaggt cccagcggcg aaggcgaggg gtcctctccg gggcaaagtc tgaaactgac 25560
tccggggcta tggacctccg cttaccttcg caagttcgcc cccaaagact accaccccta 25620
tgagatcagg ttttatgaag accaatcaca gccccccaag gccgaactga cggcctgcgt 25680
catcacccag ggggcaatct tggcccaatt gcaagccatc caaaaatccc gccaagaatt 25740
tttgctgaaa aagggacacg ggatctatct agacccccag accggtgagg agctgaatac 25800
acgcttccct caggatgccc cgaggaggca agagaatgaa agttcagatg ccgcccgagg 25860
aggagctgga agactgggac agtcaggcag aggaggaaga ctgggacagc caggcagaag 25920
aggaggacag cctggaggag gacagtctgg aggaaggcga ggagcccaag gaagaggcag 25980
ccgccgccag accatcgtcc tcggcggtgg agacaagcaa ggtcccagac agcacggcta 26040
ccacctccgc tccagctcaa ggggccgctc ggcgacccaa cagtagatgg gacgagacgg 26100
gtcgcttcca gaaccccacc accgtcaaga ccggtaagca ggagcggcag ggatacaagt 26160
cctggcgggg gcataaaagt gccatcatcg cttgcttgca ggagtgtggg ggcaatatat 26220
cctttgccag acgctacctg ctattccatc acggggtgaa tttcccccgc aacatcttgc 26280
attactaccg tcacctccac agcccctact accagcagca agagacagca gaggaaacca 26340
gcggcaactc cgagagttag aaaaccagca gctaaaaaat ccacagcggc ggcagcaggt 26400
gcaggcggac tgaggatcac cgcgaacgag ccagctcaga ccagggagtt gaggaatcgg 26460
atctttccca ccctctatgc catattccaa caaagtcggg gtcaggaaca agaactgaaa 26520
gtaaaaaaca gatctcttcg ctcgctcacc cgcagttgtt tgtatcacaa gagcgaagac 26580
caacttcagc gcactctcga ggacgccgag gctctcttca acaagtactg cgcgctgact 26640
cttaaagagt agactgcgcg cgcttggcga gaaaaggcgg gaattacgtc acctcttggc 26700
cacacctgtg cttcattatg agtaaagaaa ttcccacgcc ttacatgtgg agctatcagc 26760
cccagatggg attggccgct ggcgccgccc aggactactc cacccgcatg aattggctca 26820
gcgccggtcc cgcgatgatc tcacgggtta atggtgtgag agagcaccga aaccagatac 26880
tcctagaaca gtccgccctc accgccactc cccgcaatca cctcaacccc cgtaattggc 26940
ccgccgccct ggtgtaccag gaaactcctg ctcccactac agtactactt cctcgtgacg 27000
cccaggccga agttcagatg actaactcag gtgtacagct ggcgggtggt gccaccctgt 27060
gtcgtcaccg gccaagaccg ggtataaagg gcctggtgat cagaggccga ggtattcagc 27120
tcaacgacga gtcggtgaac tcttcgcttg gtctgcgacc agacggcatc ttccaaatag 27180
ctggttgtgg gagatcttcc ttcactcctc gtcaggctgt cctgactttg gagagttcgt 27240
cctcgcagcc ccgctcgggc ggcatcggga ctctccagtt cgtggaggag tttactccct 27300
cggtctactt caaccccttc tccggttctc ctgggcttta cccggacgag ttcatcccga 27360
actacgacgc catcagtgaa gcggtcgacg gctacgatta atgtctaatg gtggcgcggc 27420
tgagctagct cgactgcgac acctagacca ctgccggcgc tttcgctgct ttgctcggga 27480
tctctgcgag ttcatctact tcgagtaccc tgacgaacat cctcagggac ctgcccacgg 27540
agttcggatt accattgaag gggctatcga ttctcacctg cttcggatct tcaccgctcg 27600
gccagtgcta gttgagcgca accagggcga caccaccatc tccctctgct gcatttgtga 27660
caaccccgga ttgcatgaaa gcttttgttg tcttctttgt actgagtata ataaaagctg 27720
aaattagaga ctactccgga ctctcttgtc gtctgaacaa caccaaccag acccttcact 27780
tcagcgggaa ccagactact cttcactgta aggcttataa ctataagtat cttacttgga 27840
tatacaaagg aacaccgttt gctgtggtaa acaggtgctc caacgacggt gttctcctca 27900
ccttcctagg caacttctcc aactttacct tttctgttcg cagaaacaag cttaccctcc 27960
ttcagcccta ctttcctggg atctatacct gcctcagtgg accttgcaac cacacttttc 28020
acctgattga aaactctacc cttaccttcc cagcgccaat ccctactaac agctcggagt 28080
ccaactcttc cattaccgct gatactaaca ctcctaaaac cggaggtgag ctccgcagcc 28140
ttcccccggc tgcagataac ccttgggtgg tagcgggatt tgtagcgcta ggaatagttg 28200
cgggtgggct cgcgttcgtc ctctgctacc tataccttac ctgctgctca tatttagtag 28260
tactgtgctg ttggtttaga aaatgggggc gctactaatc acacttgctt tactttcgct 28320
tttgggtctg agctcggcta atagcgagaa accaagctgt ctagaaacaa actctccaga 28380
ctgtgtggtt cctcatgggc tctcagaccc agctgatgat ccatgcttaa cttttgaccc 28440
agaaaaaaac tgctcggtga ctatgcagcc ctatgcttac atgtgcacat ctgttataaa 28500
gtgcggatgg ggctgtaaac cgattgaaat tacccacaaa ggcaaaacct ggaataatag 28560
tttgtttaac acatggcagc ctggagacga gcagtggtat acggccggcc actggtggag 28620
atgactgacc ccatggaaaa ctcctctgcc aacgacctgg acatggacgg ccgttcatct 28680
gagcagcgac tggtccagat gcgcattcgc cagaagcagg aacgcgccgc cagagagctc 28740
aaggatgcca ttgaaattca cctgtgcaag aagggcatct tttgcttggt taagcaagca 28800
aagatttctt atgaaatcac tgacaacgac caccgcctgt attatgagct cggtccacag 28860
cggcagaaat tcacctgcat ggttggagtc aaccccatag tcatcactca gcaggctgca 28920
gaaattaaag ggtgcatcca ctgttcctgt gattcccaag aatgcgtcca caccatagtc 28980
aagaccctct gcggccttcg agatcttctt ccaatgaact aaccccttcc cccaacccaa 29040
taaaacattg gttttaatca taataaaaaa tcacttactt taaatctgaa acagtgtctc 29100
cgtccaagtt ttcttgtagc accacttcac tcccctcttc ccagctctgg tactgcaagc 29160
cccggtgggc tgcaaacttt ctccacacct taaaagggat gtcaaattcc tcttgtccaa 29220
caatcttcat tgtctcttcc tagatgtcca caaagcgcgc gcgggtggaa gatgactttg 29280
accctgtcta cccatacgat gctgagctgg caccgtctgt acccttcatc gcccctccct 29340
tcgtttcgtc agacggattt caagaaaaac ccctgggagt tctgtcccta agactagcca 29400
acccagtcac tactaaaaat ggggaactca cacttaaact gggagatggg gtgggcatag 29460
actcagatgg aaacctcaca gcacagacag ttactaaagc aacatccccc cttactgttt 29520
ccaataacgc aattgcactt aacatggaca aaccttttta cagtagcaat ggaaaactat 29580
ccttacaagt tacatcacca ttaaagatag tcgactcttt aaatacattg gctattggct 29640
atgggcaagg cttaggacta aacaatagtg ctcttgctgt gcaattagca tctcccctta 29700
catttgacag caacagcaaa attaaaataa atttgggaag cgggccatta aaaattaatg 29760
cgaataaact gtcaattaac tgcctaaggg gtgtatatgt aacaactgac ggaacttcca 29820
ttgaaacaaa tataagctgg gcaaaaggaa tgaggtttga aggtaatgcc atggctgtaa 29880
acgttgacag caccaaaggt ctacaatttg gcactaccag cacagaatca ggagtcacta 29940
acgctttccc tatccagtta aagattggat ctggtcttag ttttgacagc acaggagcac 30000
ttgtagcttg ggataaggat aatgacaagc ttacactgtg gacaaccgct gacccatcac 30060
ctaattgtac catatataca gacaaggatg ctaaacttac actttgtctt acaaaatgtg 30120
gcagtcaaat actaggcagt gtttcagtac tggctgttaa agctggaacc ctacagccaa 30180
tcagtgaaaa aataggtact gctttggttt cactaaaatt taataacaac ggtgtattgt 30240
taagcaactc cacattaagt aatgaatact ggaactacag gaagggagat gtcacaccag 30300
ccgaagccta tactaatgct gtgggtttta tgccaaacat caaggcatat cctaaaaaca 30360
caaactctgc ctcaaaaagc cacattgtag gacaagtgta ccttaatgga gatgaaacta 30420
aaccaatgca tttaatcatt acatttaatg aaaccagtga tgaaacatgc acatattcca 30480
taacgttcca atggaaatgg aacattggaa catacaccag cgacaccctt gcaacaagct 30540
cctttacctt ttcttacatt gcccaagaat aaaaactgca gacaacaata aagtttaaat 30600
gttttattta aacagtttca cagaacccta gtattcaacc tgccacctcc ctcccaacac 30660
acagagtaca cagtcctttc tccccggctg gccttaaaaa gcatcatatc atgggtaaca 30720
gacatattct taggtgttat attccacacg gtttcctgtc gagccaaacg ctcatcagtg 30780
atattaataa actccccggg cagctcactt aagttcatgt cgctgtccag ctgctgagcc 30840
acaggctgct gtccaacttg cggttgctta acgggcggcg aaggagaagt ccacgcctac 30900
atgggggtag agtcataatc gtgcatcagg atagggcggt ggtgctgcag cagcgcgcga 30960
ataaactgct gccgccgccg ctccgtcctg caggaataca acatggcagt ggtctcctca 31020
gcgatgattc gcaccgcccg cagcataagg cgccttgtcc tccgggcaca gcagcgcacc 31080
ctgatctcac ttaaatcagc acagtaactg cagcacagca ccacaatatt gttcaaaatc 31140
ccacagtgca aggcgctgta tccaaagctc atggcgggga ccacagaacc cacgtggcca 31200
tcataccaca agcgcaggta gattaagtgg cgacccctca taaacacgct ggacataaac 31260
attacctctt ttggcatgtt gtaattcacc acctcccggt accatataaa cctctgatta 31320
aacatggcgc catccaccac catcctaaac cagctggcca aaacctgccc gccggctata 31380
cactgcaggg aaccgggact ggaacaatga cagtggagag cccaggactc gtaaccatgg 31440
atcatcatgc tcgtcatgat atcaatgttg gcacaacaca ggcacacgtg catacacttc 31500
ctcaggatta caagctcctc ccgcgttaga accatatccc agggaacaac ccattcctga 31560
atcagcgtaa atcccacact gcagggaaga cctcgcacgt aactcacgtt gtgcattgtc 31620
aaagtgttac attcgggcag cagcggatga tcctccagta tggtagcgcg ggtttctgtc 31680
tcaaaaggag gtagacgatc cctactgtac ggagtgcgcc gagacaaccg agatcgtgtt 31740
ggtcgtagtg tcatgccaaa tggaacgccg gacgtagtca ttctcgtact ttgagtggca 31800
aaaccttgct ctcgaacagc acacgtctcg tcgcctcctg tcccttctct tcgccttttc 31860
agtgtgatag ttgtaataca gccattcacg aagctcagtc agaagatctt cagcgtctgt 31920
tgtcaaaaac aatccatcca atctgattgc tttcaaaaca tcacaaacag tcgaataagc 31980
caaacccatc caggcaatgc aattattttg gttatccaca atgggagggg gcggaagaca 32040
tggaagaggc ataattaatt ttttaatcca atcgatcacg cagcacttca aaatgaagat 32100
cgcgaaggtg acacctttca cccccactgt gttgatgaaa aataacagcc aagtcaaaat 32160
tgatgcggtt ttcaaggtgc tcgactgtag catcaagcag agcttccaca cgcacgtcca 32220
caaataacag aatagcaaaa gcgggaggag gaagtaaatc ctcaatcatc atagtacagt 32280
ccatcaccat ccctaaataa ttttcatcct tccagccttg gactatattt ttaaactgct 32340
cttgtaaatc caaaccacac atgtggaaaa gttcccaaag agctccctca actaccattc 32400
ttaagcacac cttcatagtg acaaaatatc ttgttcctct gtcacctgca gcaaattaca 32460
aagtccaata ttaggatcta tgcccagaga tctaagctca tccctcaatt ccaactgtaa 32520
aaaggcttcc agatctgccc taacttgttc agccagtggg ctccctggaa taagcgtggg 32580
agaagccaaa ctgcaaaaca gacgcatgcc gccataatta ccaccagaaa acactacgtt 32640
acagtatgca tgctgattca ttccagtaat ttcatccagt gtattggata caaaaaaagg 32700
caagcactct ctcactaatt gtattatgga gacattatca cacaggtaac aatttaaagg 32760
ttgtggaaca ataatgcagt aagtaaccac ggtgcgctcc aacatggtta gtaattttta 32820
gttctgaaaa acaaaacata caaaaaatta tatcatactc atttggcgaa ctggtggaaa 32880
aatgacccta tctagcacaa ggcaagccac tggatcacca atgcgcccct cataaaacct 32940
gtcatcatga ttaaaaagca acaccgaaag ctcttcccta tgtcctgcat gaatgattct 33000
agctgaggaa tataagccag cgcaattagt atctgttaaa gaaaaaaaac ggccaacata 33060
gcctctagga attagcacac ttaatcttaa agacattact gccatccccc ttggatttaa 33120
ggtaaaattt acaggagcat agaaaatata ctgatttccc tcctgcacag gcagcatagc 33180
accaggtccc tctaaaaaca cacacaaagc ttctgcagcc atagcttacc gcgcaaacca 33240
ggcacagcag tgagctaaaa ggacaaagct ctaactcact agccaacctg gcgcacaata 33300
tatagttagt ccttacactg acgtaaccga ccaaagtcta aaaaccccgc caaaaataca 33360
cacacgccca aaaaacgccc cgtgagtcaa aaaacagttt cacttcctcg ttacacccaa 33420
aacgtcgtca cttccggatt cccacggttc gtcacttccg gagctccttg cttaattaac 33480
cccgcccaaa acgtcatcgt ccgcgtcacg ccgccccgcc ccgcgaccgt tgaccccggg 33540
ccaatcaccg cacatcccgc aaaattcaaa ctcgtctaat ttgcatattg gcacactgcc 33600
catataaggt atattattga tgatg 33625
<210> 23
<211> 34616
<212> DNA
<213> 人工序列
<220>
<223> BLY6.RSVF-2A-GLuc腺病毒载体
<400> 23
catcatcaat aatatacctt atatgggcag tgtgccaata tgcaaattag acgagtttga 60
attttgcggg atgtgcggtg attggcccgg ggtcaacggt cgcggggcgg ggcggcgtga 120
cgcggacgat gacgttttgg ggaggaggag ctatgttgca agtaatcgtg ggaaatgcga 180
cgtaaaacga ggtggagttt aaacacggaa gtagacaatt ttcccgcgct gtttgacagg 240
aaatgatgtg tttttgggcg gatgcaagtg aaaattctcc attttcgcgc gaaaactaaa 300
tgaggaagtg aaattctgag taattctgag gttatcacag ggcggagtat ttaccgaggg 360
ccgagtagac tttgacccat tacgtggagg tttcgattac tctatttttc acctaaattt 420
ccgcgtactg tgtcaaagtc cgtgttttta cgcgatcgct caatattggc cattagccat 480
attattcatt ggttatatag cataaatcaa tattggctat tggccattgc atacgttgta 540
tccatatcat aatatgtaca tttatattgg ctcatgtcca acattaccgc catgttgaca 600
ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata 660
tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga 720
cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt 780
ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt 840
gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca 900
ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt 960
catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt 1020
tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca 1080
ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg 1140
cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat 1200
cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag 1260
cctccgcggt tggccgtgct cttcctgacg ggtaggtgtc ccctaaccta gggagccaac 1320
catcgggggg ccttctccct aaatccccgt ggcccaccct cctgggcaga ggcagcaggt 1380
ttctcactgg ccccctctcc cccacctcca agcttggcct ttcggctcag atctcagccc 1440
acagctggcc tgatctgggt ctcccctccc accctcaggg agccaggctc ggcatttcgt 1500
cgacatggaa ctgctgatcc tgaaggccaa cgccatcacc accatcctga ccgccgtgac 1560
cttctgcttc gccagcggcc agaacatcac cgaggaattc taccagagca cctgtagcgc 1620
cgtgtccaag ggctacctga gcgccctgcg gaccggctgg tacaccagcg tgatcaccat 1680
cgagctgagc aacatcaaaa agaacaagtg caacggcacc gacgccaaaa tcaagctgat 1740
caagcaggaa ctggacaagt acaagaacgc cgtgaccgag ctgcagctgc tgatgcagag 1800
cacccccgcc accaacaacc gggccagacg ggagctgccc cggttcatga actacaccct 1860
gaacaacgcc aaaaagacca acgtgaccct gagcaagaag cggaagcggc ggttcctggg 1920
cttcctgctg ggcgtgggca gcgccattgc tagcggagtg gctgtgtcta aggtgctgca 1980
cctggaaggc gaagtgaaca agatcaagtc cgccctgctg agcaccaaca aggccgtggt 2040
gtccctgagc aacggcgtgt ccgtgctgac cagcaaggtg ctggatctga agaactacat 2100
cgacaagcag ctgctgccca tcgtgaacaa gcagagctgc agcatcagca acatcgagac 2160
agtgatcgag ttccagcaga agaacaaccg gctgctggaa atcacccgcg agttcagcgt 2220
gaacgccggc gtgaccaccc ccgtgtccac ctacatgctg accaacagcg agctgctgag 2280
cctgatcaac gacatgccca tcaccaacga ccagaaaaag ctgatgagca acaacgtgca 2340
gatcgtgcgg cagcagagct actccatcat gtccatcatc aaagaagagg tgctggccta 2400
cgtggtgcag ctgcccctgt acggcgtgat cgacaccccc tgctggaagc tgcacaccag 2460
ccccctgtgc accaccaaca ccaaagaggg cagcaacatc tgcctgaccc ggaccgaccg 2520
gggctggtac tgcgataatg ccggcagcgt gtcattcttt ccacaagccg agacatgcaa 2580
ggtgcagagc aaccgggtgt tctgcgacac catgaacagc ctgaccctgc ccagcgaggt 2640
gaacctgtgc aacgtggaca tcttcaaccc taagtacgac tgcaagatca tgacctccaa 2700
gaccgacgtg tccagctccg tgatcacctc cctgggcgcc atcgtgtcct gctacggcaa 2760
gaccaagtgc accgccagca acaagaaccg gggcatcatc aagaccttca gcaacggctg 2820
cgactacgtg tccaacaagg gcgtggacac cgtgtccgtg ggcaacaccc tgtactacgt 2880
gaacaaacag gaaggcaaga gcctgtacgt gaagggcgag cccatcatca acttctacga 2940
ccccctggtg ttccccagcg acgagttcga cgccagcatc agccaggtca acgagaagat 3000
caaccagagc ctggccttca tcagaaagag cgacgagctg ctgcacaatg tgaatgccgt 3060
gaagtccacc accaatatca tgatcaccac aatcatcatc gtgatcatcg tcatcctgct 3120
gtccctgatc gccgtgggcc tgctgctgta ctgcaaggcc cggtccaccc ctgtgaccct 3180
gtccaaggac cagctgagcg gcatcaacaa tatcgccttc tccaacggac gcgtgaccga 3240
gctgctttac cggatgaagc gggctgagac atattgcccg agacccctgt tggcaatcca 3300
tcctactgag gctcgccaca aacagaaaat cgtggccccc gtcaaacaga cactcaattt 3360
tgacttgttg aaacttgcag gagatgttga gtcaaacccc gggcctatgg gcgtcaaggt 3420
cctgttcgct ctgatttgta tcgctgtcgc tgaagctaag ccaaccgaga ataatgaaga 3480
ctttaatatc gtggccgtgg cttctaactt cgctaccaca gacctggatg cagacagggg 3540
aaagctgcca ggcaagaaac tgcccctgga ggtcctgaag gagatggaag caaatgcccg 3600
gaaagccggg tgcacaagag gatgcctgat ttgtctgagc cacatcaagt gcactcctaa 3660
gatgaagaag ttcatccccg gccggtgcca tacctacgag ggcgataagg aatccgccca 3720
gggaggaatc ggagaggcta tcgtggatat tcccgaaatc cctggcttca aagacctgga 3780
gcccatggaa cagtttattg cacaggtgga tctgtgcgtc gactgtacta ccggatgcct 3840
gaagggactg gcaaacgtcc agtgtagcga cctgctgaag aaatggctgc ctcagcgatg 3900
tgctacattt gccagcaaga ttcagggcca ggtggacaag attaagggag caggaggcga 3960
ctgataattc tagacgagat ccgaacttgt ttattgcagc ttataatggt tacaaataaa 4020
gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 4080
tgtccaaact catcaatgta tcttatcatg tctgcgatcg cgatggcctg tgtttgagca 4140
taatgtactg accaggtgta acgttcatct gggtggtcgt agaggaatgt tcatgccata 4200
ccaatgcaat tttaatcatg tgaggatctt gatggagccg caagcgtttt ccagagtcag 4260
cttgactgga atctttgaca tgtgtgtgga agcatggaag atcttaagat atgatgatac 4320
caaatccaga tgccgcgcat gcgagtgcgg gggcaggcat gccaggttcc aacctgtatg 4380
tgtggaggtg accgaggagc tgagaccaga tcatttggtg ctgacctgca ctggtgcgga 4440
gttcggttcc agtggtgaag aaactgatta aagtgagtag tgggatgtta taaaagtgac 4500
cataaggtga tgtgagatgg acaaatttgg taatttttat gtatttttgt cttgcagcca 4560
tgagtgggag cgcttccttt gaagggggcg tctttagccc ttatctgacg gggcgtctgc 4620
ctcattgggc tggagtgcgt cagaatgtga tggggtctac agtggatgga agacctgttc 4680
agcctgctaa ttcttctact ctgacttatg ctactatgac ttcctcgcct ttggatgcag 4740
ctgcagctgc tgccgcttct gctgccgcca acactgttcg ggggatggcc ttggagatgg 4800
ggtattatgg aactgtagtg gccaacacca ctaccccaaa taaccccaca gccttgaatg 4860
aggacaagct gctagttctc atgtcccagc tggagtcttt gacccaacgc ctgggcgatc 4920
tagctcagca ggtgtcccag ctgaaggagc agactcaagc tgcaattacc actgcgaggg 4980
gaaattaaaa aaattcaaag aatcaataaa taaaccgaga ctttgttgat tttaaagtgt 5040
gtcattcttt atttaatttt tcgcgcgcga tatgccctgg accaccggtc tctatcattg 5100
aggacacggt ggatcttttc tagaacccga tagaggtggg attggatgtt gaggtacatg 5160
ggcataagac catctttggg gtgtagatag ctccactgca gagcctcatg ctccggggtg 5220
gtgttgtata taacccagtc atagcatggg cgttgggcat gatgttgcac aatatcttta 5280
aggaggagac taatggccac tgggagaccc ttggtgtaag tgtttacaaa tctattaagc 5340
tgggacgggt gcatccgagg tgagataatg tgcattttgg attggatttt tagattggca 5400
atgtttcccc ctagatctct cctgggattc atgttatgca agaccactag aacagtgtat 5460
ccggtgcact tagggaattt gtcatgaagt ttggagggga aagcatgaaa aaatttagac 5520
acacccttgt gtcctcccaa gttctccatg cactcatcca taataatggc aatgggccca 5580
tgggcggcgg cacgggcgaa cacgttcctg ggatctgaca catcatagtt gtggtcttgg 5640
gtcaggtcat cataagccat tttaataaac ttggggcgga gggtgccaga ttgggggatg 5700
aatgttccct cgggccccgg aacatagttt ccttcacata tttgcatttc ccaggctttt 5760
agttcagagg gggggatcat gtccacctgt ggagcgatga agaagacggt ctcgggggcg 5820
ggggtgatta agtgggagga cagcaagttc ctaagcagct gtgacttgcc acacccagtg 5880
ggaccgtaga tgacccctat aacaggttgc agatggtagt ttagggaaag acagctgccg 5940
tcctctcgca ggaggggggc gacctcgttc atcatttccc tcacatgcat gttttcccgc 6000
acaagttccg ataggaggcg ctctccaccc agggaaagga gttcttgaag agatgagaaa 6060
tttttcaagg gttttaagcc atcagccatg ggcattttgg agagggtttg ttgcaagagt 6120
tcaaggcggt cccagagttc ggtgatgtgt tctatggcat ctcgatccag catacttcct 6180
cgtttctggg gttgggacgg ctgcgggagt atggaaccag gcgatgggcg tccagcgctg 6240
ccagtgtccg gtccttccac ggtcgcagcg tccgagtcag ggtcgtttcc gtcacggtga 6300
aggggtgcgc gcctggctgg gcgcttgcga gggtgcgctt caggctcatc ctgctcgtgg 6360
agaaccgctg ccgttctgcg ccctgtgcat cggccaggta gcaattaacc atgagttcgt 6420
agttgagcgc ctctgccgcg tggcctttgg cgcgcagctt acctttggaa gtcttctgac 6480
aggtgggaca gtagagacac ttgagagcat agagttttgg ggctagaaag accgattctg 6540
gggagtatgc atcggcccca caggaggcgc agacggtttc gcattccacc agccatgtaa 6600
gatcgggctc gttggggtca aaaacaagtt ttccgccatg ttttttgatg cgtttcttac 6660
ctttgctttc catgagttcg tgcccccgtt gggtgacaaa gaggctgtcc gtgtccccgt 6720
agactgactt tatgggcctg tcctcgagcg gcgtgccgcg gtcctcttcg tagaggaact 6780
cggaccactc tgagacgaaa gcacgtgtcc aggccagcac aaaggaggct atatgggagg 6840
ggtagcgatc gttgtcaacc aaggggtcta ctttttccaa ggtgtgtaaa cacatgtccc 6900
cttcttccac atccaggaag gtgattggct tgtaagtgta tgccacgtga cctggggtcc 6960
cagacggggg ggtataaaag ggggcgggtc tctgctcgtc ctcactgtct tccggatcgc 7020
tgtccaggag cgccagctgt tgaggtaggt attccctctc gaaggcgggc ataacctccg 7080
cactcaggtt gtcagtttct aggaacgagg aggatttgat attgacagtg cctgccgaga 7140
tgcctttcat gagactgtcg tccatttggt cagaaaagac aatctttttg ttatcaagtt 7200
tggtggcgaa ggatccatac agggcattgg aaagcagttt ggcaatggag cgcatggttt 7260
ggtttttttc tttgtctgcg cgctctttgg cggctatgtt gagttggaca tattcgcggg 7320
ccagacattt ccattgtgga aatatggtag ttaattcatc tgggacgatt ctgactttcc 7380
agcctctgtt atgcagggta atcagatcca cactggttgc cacttctcct ctaagtggtt 7440
cattagtcca gcatagtcgc cccccttttc gagaacagaa agggggtagg ggatctagca 7500
tgagttcgtc tggggggtct gcatctatgg tgaaaatccc aggaaggaga tcttcgtcaa 7560
aatagctgat ggtggcgggg tcatccagag acatttgcca ttctcgagca gccagagcgc 7620
gctcgtaggg gttaagggga gtcccccatg gcatgggatg ggtgagtgca gaagcataca 7680
tgccacagat gtcatagaca tagagcggct cttccagaat ccctatgtaa gtgggataac 7740
atcgcccccc tctgatgctg gctcgcacat aatcatagag ttcatgtgag ggcgctagaa 7800
gacccgagcc caggttggtg cggttgggtt tttctgctct gtagaggatc tggcgaaaga 7860
tggcatggga gtttgatgag atggtgggtc tttggaagat gttgaaatgg gcatgaggca 7920
gtcccacaga gtcccttatg aagtgagcat aggagtcttg cagtttggcc accagctcgg 7980
cggtgaccag cacatccaaa gcacagtagt cgagggtctc tttgatgatg tcatagttag 8040
gttccccttt cttttcccac agctcgcggt tgagaaggta ttcttcgcga tccttccagt 8100
actcttcgag ggggaacccg tccttgtctg aacggtaaga acccagcatg taaaattgat 8160
tgacagcttt gtaggcacaa caccccttct ccacggggag tgagtatgct tgcgcggctt 8220
tgcgcagaga ggtgtgagta agggcgaaag tgtccctgac catgactttg aggaactgat 8280
gcttaaagtc tatgtcatcg caggccccct gctcccacag ttggaagtcc actcgctttt 8340
tgtaggcggg attgggcaaa gcgaaagtaa catcgttgaa taggatcttt ccagccctgg 8400
gcatgaagtt gcgagtaatg cgaaaaggct gaggcacttc tgccctgttg ttgataactt 8460
gggcagccaa gacgatctcg tcaaagccgt tgatgttgtg acccacaatg taaagttcta 8520
cgaagcgtgg gcgtcccttg atgtggggca gttttttaag ctcttcgtag gtcaagtcgt 8580
cagggtcagc gattccatat tgctccaaag cccagtcagg caggtgagga ttagcatgaa 8640
ggaaagaggt ccaaagatcc acggccagag ctgtttgtaa gcggtctctg tactgacgga 8700
aatgtcggcc taccgccatt ttttcaggag taacacagta aaaggtgcgc gggtcctttt 8760
cccagcgatc ccattgaagt tgcaaggcta ggtcgtgggc gaggttgacg agctgttcgt 8820
cccccgaaag tttcatgacc agcatgaaag ggacaagctg cttgccaaag gaccccatcc 8880
aggtgtaggt ttccacatcg taggtgagga agagcctttc tgtgcgagga tgagaaccga 8940
tcgggaagaa ctggatttcc tgccaccagt tggaggaatg gctgttgata tgatggaagt 9000
agaactccct acggcgcgcc gagcattcgt gcttgtgctt gtacagacgg ccacagtact 9060
cgcagcgctg cacgggatgc acctgatgaa tgagctgtac ctggcttcct ttgacaagaa 9120
atttcagtgg gaagttgagg cgtggcgtct gcatctcgtg ttgtattacg tcctggctat 9180
tggtctggcc atcttctgtc tcgatggtgg tcatgctgac gagcccgcgc gggaggcagg 9240
tccagacctc cgcgcggacg ggtctgagag cgaggacgag agcgcgcagg ccggaactgt 9300
ccagggtcct gagacgctgc ggagtcaggt cagtagggag agtacatagg tttacttgca 9360
taagtttttc cagggcatgt gggaggtcaa gatgatattt gatttctact ggcgagttgg 9420
tggagacatc gatggcttgc agggtcccgt gcccctgggg tgctaccacc gtcccttttt 9480
ttttcttgat cgggggcggt gttgcttctt gcatggtaag gtcgtcttct agaagcggcg 9540
gcgaggtcgc gcgccgggtg gcagtggcgg ttctggacct ggaggtagag gcggtagagg 9600
tacgtcggcg ccgcgcgcgg gtaggttctg gtactgcgcc ctgagaagac ttgcgtgagc 9660
gacgacgcgg cggttgacgt cctggatctg acgcctctgg gtgaatgcta ccggacccgt 9720
gagcttgaac ctgaaagaga gttcaacaga atcaatttcg gtatcgttga cggctgcctg 9780
ccgcaggatt tcttgtacgt cgcccgagtt gtcttggtag gcgatctcgg ccatgaactg 9840
ctcgatctct tcttcttgga gatctccgcg gcccgctcgt tctacggtgg cagcaaggtc 9900
gttggagatg cgccccatga gctgtgagaa tgcattcatg cccgcctcgt tccagacgcg 9960
actgtagacc acggctccct cgggatctct ggcgcgcatg accacttggg cgaggtttag 10020
ttccacgtgt ctggtgaaga ccgcatagtt gcagagacgc tggaagaggt agttgagcgt 10080
ggtggcgatg tgctcggtga caaagaaata catgatccag cgacgaagcg gcatctcgct 10140
gatatcgccc agggcttcca accgttccat ggcttcgtaa aagtccacgg cgaagttgaa 10200
aaactgggag ttgcgagcgg acacggtcaa ctcctcctcc agaagacgga tgagctcggc 10260
gatggtggcg cgcacttcgc gctcaaaggc tcccgggatc tcttcctcct cttcttcttc 10320
caactcttcc tccactaaca tctcttctac ttcctcctca ggcggcgggg gtggaggagg 10380
gggcgcgcgg cgacgccggc gacgcacggg cagacgatcg atgaagcgtt cgatcacttc 10440
tccgcggcgg cgacgcatgg tctcggtgac ggcgcgcccg tcctccctgg gtcgcagagt 10500
gaagacgccg ccgcgcagct ccctgaaatg gtgactggga gggtccccgt ttggtaggga 10560
cagggcactg atgatgcatc ttattaattg ccctgtaggg actccgcgca aggacctgag 10620
cgtctcgaga tccacgggat ctgaaaatct ctgaacgaag gcttcgagcc agtcgcagtc 10680
gcaaggtagg ctgagcactg tttcttcggg gcgggctgct gagctagagg gttgtacgat 10740
gctgctggtg atgaagttaa aataggcagt tctgagacgg cggatggtgg cgaggagcac 10800
caggtctttg ggtccggctt gctggatgcg caggcggtcg gccattcccc atgcattatc 10860
ttggcacctg gccagatctt tatagtagtc ttgcatgagt cgctccacgg gcacttcttc 10920
ttcgcccgct ctgccgtgca tgcgtgtgag tccgtaccct ctctgtggtt ggacgagcgc 10980
caggtcggca acgacccttt cggctagaat ggcttgctgc acctgggtga gggtgttctg 11040
gaaatcatca aagtccacaa agcggtggta ggcccccgtg ttgatggtgt aggagcagtt 11100
ggacatgacc gaccagttga ctgtctggtg tcctggtcgt acgagttccg tgtacctgag 11160
ccgcgagtat gcgcgggagt cgaagatgta atcgttgcag gttcgcacca ggtactggta 11220
gccgatgagg aagtgaggcg gcggctggcg gtagagaggc catcgttcgg tggcgggcgc 11280
gccgggcgct aggtcttcta gcatgagacg gtggtatccg tagacgtacc tggacatcca 11340
ggtaataccg gcggcggtgg tggaggcgcg cggaaactct cgcacgcggt tccagatgtt 11400
gcgcagcggc atgaagtagt tcatggtggg cacggtctgg cccgtgaggc gcgcgcagtc 11460
attgatgctc tagatacggg caaaaacgaa agcgttgagc ggttcccttc cgtggcctgg 11520
aggaacgcga acgggttagg tcgcagcgta ccctggttcg agactaaaga aagcgagcaa 11580
ctcgaaccgg cagagtcgcg gctaacgggt attggcaatc ccgtctcgac ccaagccagc 11640
aaatccagga tacggatggg ggcccctttt gtttttcagg gcatgagtca ccggttaagg 11700
tttacaacgg ctgtttcatg cctttagaag tggctcgcgc ccgtagtctg gagaatcaat 11760
cgccagggtt gcgttgcggc gtgccccggt tcgagcctgc agcttgagtc ggccggtgac 11820
cgcggcaaac gagggcgtgg cggccccgtc gtttctaaga ccttgctagc cgacctctcc 11880
agtttacggg aacgagcccc cttttatttt ttttgttttt gccagatgca tcccgtactg 11940
cggcagatgc gcccacagcc cccacagcag cagcagcagg ctggcctacc ttctctacct 12000
cagccgctac ctgcaactac cgcggtggcc gctgtaagcg gggccggaca gcaggcggct 12060
cctcaatatg aattggactt ggaagagggc gagggattgg caagattggg ggcgccctcg 12120
cccgagcgcc acccgcgggt gcagatgaaa aaggacgttc gcgaatctta cgtgcccaag 12180
cagaatctgt tcagagacag aagcggcgag gagcccgagg agatgcgcgc gtcccgtttt 12240
aacgcgggtc gcgagctgcg acaaggactg gatcgaaaac gggtgttgag ggatgatgat 12300
tttgaggtgg atgaaatgac agggatcagc cccgctcgcg ctcacgtggc tgcagctaat 12360
ctggtgacag cttatgagca gaccgtgaag gaggaaagca acttccagaa atcattcaat 12420
aaccacgtgc gcaccctgat cgcacgcgag gaggtgaccc tgggcctgat gcacctgtgg 12480
gatctgctgg aagccatagt gcagaacccc actagcaaac ccctgactgc tcaactgttt 12540
ctggtggtgc agcacagcag ggataatgag gcattcagag aggcgctgct gaatatcact 12600
gaacctgagg ggagatggct gctggatctg gtgaatatcc tgcagagcat tgtagtgcag 12660
gaacgcagct tgcctttgtc cgagaaggtg gcggcgatca attactctgt gctgagtctg 12720
ggcaaatact atgccaggaa gatctacaaa accccttacg tgcccataga caaggaagtg 12780
aaaatagatg ggttttacat gcgcatgacc ctgaaagtgc taaccctgag cgatgacttg 12840
ggagtgtacc gcaacgacag gatgcaccgc gcggtgagcg ccagcaggag gcgcgagctg 12900
agcgacaaag aattaatgca cagcttgcaa cgagccctga cgggagccgg gacggagggg 12960
gagaactact ttgacatggg tgcagacttg cattggctgc ctagtcgcag ggcattggaa 13020
gcggcaggcg atgggcccta tgtagaggaa gtagtagacg aggacgatga ggagggcgag 13080
tacctggaag actgatggcg cgacccgtat ttttgctaga tggaacaggc gccggaccct 13140
gcgatgcggg cggcgctgca gagccagccg tccggcatta attcctcgga cgattggacc 13200
caggccatgc aacgcatcat ggcgctgacg acccgcaacc ccgaagcctt tagacagcag 13260
cctcaggcca accgcctttc ggccatcctg gaggccgtgg tgccctctcg ctccaacccc 13320
acccacgaga aggttctggc catcgtgaat gccctggtgg agaacaaggc catccgctcc 13380
gatgaagccg ggctggtata caacgccttg ctcgagcgcg tggctcgcta caacagcagc 13440
aatgtccaga ctaacctgga caggatggtg accgacgtgc gcgaggccgt gtcccagcgc 13500
gaacggttcc atcgcgagtc taacctgggt tccatggtag cgctgaacgc tttcctcagt 13560
tcccagcctg ccaatgtgcc ccggggacag gaagactata ccaactttat tagcgccctg 13620
agactcatgg tagccgaggt tcctcagagc gaggtgtacc agtccggtcc agactacttt 13680
ttccagacaa gcaggaacgg tatgcagaca gtgaacttaa gccaggcttt caagaacctg 13740
caagggctgt ggggagtcca agctccagtg ggcgacaggg cgaccgtgtc gagcctgttg 13800
actccaaatt cccgtttgct gctgctgctg gtgtccccct tcactgacag cggcagcata 13860
aacagaaact cctacttggg ctacctgata aacttgtatc gcgaagctat aggtcaggcc 13920
cacgtggacg aacagaccta tcaggagatc actaatgtga gtcgcgctct gggccaggac 13980
gaccctggaa acctggaagc tactctaaac tttctgctga ccaaccgctc gcaaaaaatc 14040
cctcctcagt atacattaac tgcggaggag gaacggatct tgagatacgt gcagcagagc 14100
gtgggtctgt tcctgatgca agagggtgcg acccctagcg ccgcgcttga tatgacagcg 14160
cgcaacatgg agcccagcat gtatgccagc aacagaccat tcattaataa attgatggat 14220
tacttccatc gcgcggccgc tatgaactct gattacttca ccaatgctat tctgaacccc 14280
cattggctgc ctccgcctgg tttttatact ggcgagtatg acatgcctga ccccaacgat 14340
gggttcttgt gggacgatgt ggacagcgtg gcgttctcgc ctaccgctcc tcgtactttt 14400
tggaagaagg aaggtagtga cagaagaccc tcctccgtgc tgtcaggacg tgagggtgct 14460
gccgcggcgg tccccgatgc tgcaagcccc tttcccagtc tgccattttc actaaacagc 14520
gtgcgcagta gcgagctggg gagaataacc cgccctcgct tgctgggcga ggacgagtat 14580
ttgaatgact ccctactgag acccgagcgg gaaaagaact tccctaataa tgggattgaa 14640
agcctggtgg ataagatgag cagatggaag acctatgccc aggagcacag agatgagcct 14700
agaatcttgg gtcctacagt aggcacccgc agacgccagc gccatgatag acagcggggt 14760
ctggtgtggg acgatgagga ttctgcagat gacagcagcg tgttggactt gggcgggagg 14820
ggaggtgtgg gcaacccgtt cgcacacttg cgtccccgta ttggacgcat gatgtaaaag 14880
tgaaaataaa aaaggaactc accaaggcca tggcgaccag cgtgcgttcg ttctttctgt 14940
tgttgtatct agtatgatga ggcgcaccgt gctaggcgga tcggtggcgt atccggaggg 15000
tcctcctcct tcgtacgaaa gcgtgatgca gcaggtggcg gcggcggcga tgcaaccccc 15060
cttggaggct ccttacgtgc ccccgcggta cctggcacct accgagggga gaaacagcat 15120
tcgttattcg gaactcacac ccttgtatga caccacccgg ttgtacctgg tggacaacaa 15180
atcggcggac attgcctcgt tgaactatca gaacgaccac agcaacttct tgacaacggt 15240
ggtgcagaac aatgacttta cccccacgga ggccagcacc cagaccatca actttgacga 15300
gcgctcccgg tggggcggtc agctgaagac catcatgcac accaacatgc ccaacgtgaa 15360
cgagttcatg tttagcaaca agttcagggc tagggtgatg gtgtccagaa ccacacctaa 15420
agaggtgaca gtcacaacag actatgatgg tagtcaggac atcttggaat acgagtgggt 15480
tgactttgag ttaccagaag gcaacttctc tgccaccatg accatagacc tgatgaataa 15540
tgcaattgtt gataattacc taaaagtggg tagacagaat ggggtactgg agagtgacat 15600
aggtgttaag tttgacacta ggaactttag gcttggttgg gacccagtga cagagttggt 15660
catgcctggg gtctacacca atgaagcttt ccatcctgac atagtcctac tacctggctg 15720
cggagtggac ttcactgaga gccgcctcag taatctgcta ggcattagaa agaaacagcc 15780
attccaggaa gggttccaga tcatgtatga ggatctggag ggtggtaaca tccccgccct 15840
gcttgatgta aatgcatatg agaagagcaa ggaagataat acaaccacca caaatgaagc 15900
tgtggccgcg gcttcatcta ctgaagccaa agctgtggta gatgcttcca cttcaacaga 15960
aaacaccact gatgaaaaag tcaccagggg agatacattt gccacccctg aacaagagaa 16020
ggcagctgag gcagagtctg atattatgct tctgtccacc gatgaaaacg aaactaaaaa 16080
acaactggtt attcgagcgg tgaccaagga tagtaaggac aggagttata atgtattgtc 16140
agatggaaag aacacagctt accgtagttg gtacctggca tacaattatg gcgaccgtga 16200
gaaaggggtg cgttcttgga cactgcttac cacctcggat gtcacctgcg gcgtggagca 16260
agtctattgg tcgctaccag atatgatgca agatccagtc acctttcgct ccacacgcca 16320
agttagcaac tacccagtgg tgggcgcaga gctgctccca gtgcattcca gaagcttcta 16380
caacgagcaa gccgtctact cgcaacagct ccgccagtac acctcgctca cgcacgtctt 16440
caaccgcttc cccgagaatc agatcctcgt ccgcccgccc gcgccaacca ttaccaccgt 16500
cagtgaaaac gttcctgctc tcacagatca cgggaccctg ccgctgcgca gcagtatccg 16560
gggagtccag cgcgtgaccg ttactgacgc cagacgccgc acctgtccat acgtatacaa 16620
ggccctgggc atagtcgcgc cgcgcgtcct ttcaagccgc actttctaaa aaaatgtcca 16680
ttctcatctc gcccagtaat aacaccggtt ggggcctgcg cacacctagc aagatgtatg 16740
gaggcgctcg cagacgctcc actcagcacc ctgtgcgcgt gcgcgggcat ttccgcgctc 16800
cctggggcgc cctcaaggga cgctctcgta ctaggaccac cgttgacgat gtgatcgacc 16860
aggtggtcgc cgatgcacgt aactataccc ccgcagccgc acctgcatcc accgtggatg 16920
cggtcattga cagcgtggta gccgatgcgc gcgcctatgc tcgcgccaag agcaggaggc 16980
ggcgtattgc caggcgtcac cgagctactc cagccatgcg agctgcaaga gctttattgc 17040
ggagagccag acgtgtgggg cgaagagcca tgcgtagagc ggccagacgc gcggcttcag 17100
gtgccagcgc aggcagggtc cgcaggcgcg cggctacggc ggcagcggcg gccatcgcta 17160
gcatgaccaa accacgaaga ggcaatgtgt attgggtgcg cgacgccgcc accggccagc 17220
gcgtgcccgt gcgcacacgc ccccctcgca cttagaagat actgagcagt ctccgatgtt 17280
gtgtcccagc ggcgagatgt ccaagcgcaa attcaaggaa gagatgctcc aggtcatcgc 17340
gcctgagatc tacggtcctg cggtgaagga tgaaaaaaag ccccgcaaga tcaagcgggt 17400
caaaaaggac aaaaaggaag aagatggtga tgatgggctg gtggagtttg tgcgcgagtt 17460
tgccccaagg aggcgcgtgc agtggcgcgg gcgcaaagtg tggccggtgt tgagaccggg 17520
gaccacagtg gtctttacgc caggcgagcg ctccagcacc gtttccaaac gctcttatga 17580
tgaggtgtac ggggacgatg atattctcga gcaggcggct gatcgccttg gcgagtttgc 17640
atatggcaaa cgcagccgct cgggagccaa ggaagaggca ttgaccatcc ccttggatca 17700
tggaaatccc accccaagcc tcaaacccgt gaccctgcaa caagtgctgc ccacgccgcc 17760
acgcaagggc atcaagcgcg agggcgagga tctgtatccc accatgcagc tgatggtgcc 17820
caagcgccag aagctggaag acgtgctgga gaaaatgaaa gtggatcctg aaatccagcc 17880
tgaagtcaaa gtgaggccaa tcaagcaggt ggcgcccggt ttgggggtac aaaccgtgga 17940
tatcaagatc cccaccgagt ccatggaaat tcaaaccgaa cccatgaagc ccacctccag 18000
caccattgag gtgcagacgg atccttggat gcccgcgcct gctcctgtta ccactactac 18060
tcgaagacct agaagaaagt atggttcagc caacctgata atgccaaact atgctctgca 18120
tccatcaatc atacccactc ctggctaccg cggcactcgc tactaccgca gtcacagcac 18180
ccgccgacgt aaagcacctg ccacccgccg ccgtcgccgc cgccgtgcca ctagcaaact 18240
taccccctcg gctatggtgc ggagagtgta ccgtgatggg cgcgcagctc ctctgacact 18300
gccgcgcgcg cgctaccatc ctagcattgc catttaacaa ctctgcctcc ttgcagatat 18360
ggccctcact tgccgccttc gtattcctat tgctggctac cgcggaagaa agtcgcgccg 18420
tagaagagca gggttgtctg ggagcgggat gcgtcgccac cggcggcggc gcgccatcag 18480
caaacggttg gggggtggat ttcttcccgc tttgattccc atcatcgccg cggcgatcgg 18540
cgcgatacca ggcatagctt ccgtggcggt gcaggcctcg cagcgccact gacattggaa 18600
aaagatatct tataaataaa aatagaatgg actctgacgc tcctggtcct gtgatatgtt 18660
tttgtagacg agatggaaga catcaatttt tcatccctgg ctccgcgaca cggcacgcgg 18720
ccgtatatgg gcacctggag cgacatcggc aacagccaac tgaacggggg agccttcaat 18780
tggagcagtc tatggagcgg gcttaaaaat tttgggtcca ctataaagac ttatgggaac 18840
aaagcttgga acagcagcac agggcatgcg ctgagacaaa agcttaaaga tcagaatttc 18900
caacagaagg tggtcgatgg tatcgcctct ggaatcaatg gggtggtaga tctggccaac 18960
caggccgtgc agaaacagat taacagtcgc ctggacccgg ctcccccagc tcctattcat 19020
gagttaatgc aagtggagga agagctccct tcattggaaa agcggggcga taagcgacct 19080
cgtccagata tggaggaaac gctgctgacc aaggtggatg agccgccctc ctatgaagag 19140
gctgtaaaac tgggaatgcc cactacaaag cccattatgc ctctggccac tggagtgatg 19200
aagccatctc agtctaaacc tgcagttgct gctacattgg acttgcccgc tcccgtggcc 19260
acccccaaac ctgtcgccgc cccgaagccc accgccgtgc aacccgtggc cgtggccaga 19320
ccgcgtcccg gtggtcggcc gaatgcaaac tggcagagca ctctgaacag catcgtgggt 19380
ttgggagtgc acagtgtgaa gcgccgtcgc tgctattgat taaatatgga gtagcgctta 19440
acttgcttgt ctgtgtgtgt atatgtcgat gccgcccgcc gtgctacagc aaagagagaa 19500
ggagaagagg cgccgctgag ttcctttcaa gatggccacc ccatcgatgc tgccccagtg 19560
ggcgtacatg cacatcgccg gacaggacgc ttcggagtac ctgagtccgg gtctggtgca 19620
gttcgcccgc gccacagata cctacttcaa tctggggaac aagtttagga accctaccgt 19680
ggctcccacc cacgatgtga ccaccgaccg tagccagcgc ctgacgctgc gctttgtgcc 19740
cgttgaccgg gaggacaata cctactccta caaagtcaga tacaccctgg ctgtgggaga 19800
caacagggtg ttggatatgg ccagcaccta ctttgacatc aggggcgtgt tggacagagg 19860
acctagcttc aaaccatact ctggcactgc ctacaactcc ctggctccaa aaggagctcc 19920
aaactccagt cagtggcaac aaaaggaaaa caatggtcaa ggtgatgcaa agactcacac 19980
ctatggtgta gctgccactg gaggtattga cattgacaaa aatggtcttc aaattggaat 20040
cgatgaaact aaagaagata ataacgaaat ttatgcagac aaaacattcc aacctgaacc 20100
tcaaattgga gaagaaaact ggcaagatag cgaaaactat tatggaggca gggctcttaa 20160
accggaaacc aagatgaagc cttgctatgg ttccttcgct agaccaacta atgcaaaggg 20220
aggtcaagcc aaaattaaac cagctcaaga gggtcaacag tctatagatt atgacataga 20280
cctggctttc tttgatattc caagcactgg cggaggcaat ggcacaaatg taaatgacaa 20340
gccagatatg gttatgtata ctgaaaatgt aaatctggaa actccagaca ctcatcttgt 20400
ttacaagcca ggaacttcag atgacagttc cgaggccaat ttaactcagc aagccatggc 20460
taacagaccc aactatattg ggtttagaga taactttatt ggcgtcatgt actacaacag 20520
cactggcaac atgggagtgc ttgctggtca agcatcccag ctaaatgctg tggtggacct 20580
gcaagacaga aacaccgagc tgtcttatca gctattactt gactctctgg gcgacagaac 20640
caggtatttt agtatgtgga atcaggcggt ggacagctat gatcctgatg tgcgcattat 20700
tgaaaaccat ggtgtggaag atgaattgcc aaactattgc ttcccattgg acggagctgg 20760
cactaatgct gtttaccaag gagttaagac aaaagaggat aataatggcg aatgggaaac 20820
agacacaaat gttgcatcgc agaatcagat atgcaagggc aacatatatg ctatggagat 20880
caacctgcaa gccaacctgt ggaaaagttt cctttactcc aacgtggctc tgtacctacc 20940
agactcctac aagtacactc catccaacgt gacactccct accaacacta acacctatga 21000
ctacatgaat ggcagggtgg tgtctccatc cctggtggat gcctacatta acattggcgc 21060
caggtggtct ctggatgcca tggacaatgt caaccctttc aaccaccacc gcaatgccgg 21120
cctgcgctac cggtccatgc ttctgggcaa cggccgatac gtgcccttcc acatccaagt 21180
gccccagaaa ttcttcgcta tcaagaacct gctgcttctc ccaggctcat acacctacga 21240
gtggaacttc cgcaaggatg tcaacatgat cctgcagagt tcccttggca atgacctcag 21300
aaccgatggg gccaccatcc agtacaccag catcaatctc tatgccacct tcttccccat 21360
ggctcacaac actgcctcca ccctggaagc catgctgcgc aatgacacca atgaccagtc 21420
cttcaatgac tacctctcag ctgccaacat gctttacccc atccctgcca atgccaccaa 21480
cgtgcccatc tccatcccat ctcgtaactg ggctgccttc aggggctggt ctttcacccg 21540
cctcaagacc aaggagaccc catctctggg atcagggttc gatccctact tcgtctactc 21600
aggctccatt ccatacctgg atggaacttt ctaccttaac cacactttca agaaagtctc 21660
catcatgttt gactcttctg tcagctggcc aggcaatgac aggctgctga ctcccaatga 21720
gttcgaaatc aagcgcactg ttgatgggga agggtacaat gtggcacaat gcaacatgac 21780
caaagactgg ttcctggttc agatgctctc ccactacaac attggctacc agggcttcta 21840
catcccagaa ggatacaagg accgcatgta ctccttcttc agaaacttcc agcccatgag 21900
ccgccaggtg gtcgatcagg tcaactacaa agactacatg gcagtcaccc ttgcctatca 21960
gcacaacaac tctggctttg tgggctacct cgcgcccacc atgcgacagg gccaacccta 22020
ccctgctaac tacccatacc cgctcattgg caagactgca gtcaacagtg tcacccagaa 22080
aaagttcctc tgcgacaggg tcatgtggcg catccccttc tccagcaact tcatgtccat 22140
gggggccctt accgacctgg ggcaaaacat gctttatgcc aactccgccc acgcgctaga 22200
catgaatttc gaagtagacc ccatggatga gtccaccctt ctctatgttg tcttcgaagt 22260
cttcgacgtg gtcagagtgc accagcccca ccgcggcgtc atcgaagctg tctacctgcg 22320
cacccccttc tcagctggta acgccaccac ataagcgcct tgcttcttgc aagtggctgc 22380
agcagcatgg cctgtggatc ctccactgga tccaatgagc aagagctcag ggccatcgcc 22440
atagacctgg gctgtggacc ctatttcctg ggaacctttg acaagcggtt tccaggcttc 22500
atggctcctg acaagctcgc ctgtgccatt gtcaacacgg cagggcgcga gactggtggt 22560
gagcactggc tggcttttgg atggaacccc cgctccaata cctgctatct ctttgacccg 22620
tttgggtttt cagacgagcg cctcaagcag atctatcaat tcgagtacga ggggctcctg 22680
cgccgcagtg ccctggctac taaggaccga tgcatcactc tggaaaagtc tacccagacc 22740
gtgcagggtc cgcgctcggc tgcctgcggg ctcttctgct gcatgttcct ccatgctttt 22800
gtgcactggc ccgaccgccc catggacaac aaccccacca tgaatttgct gacgggggta 22860
cccaacaaca tgctccaatc gccccaagta gagcccaccc tgcgccacaa ccaggaggca 22920
ctctatcgct tcctgaactc ccactcatct tactttcgtt ctaaccgcgc gcgcattgag 22980
aaggccactg ccttcgatcg aatgaataat aacatgtaaa ccaaattgtg tgtggctcaa 23040
ataaacagca ctttattgtt tacatgcact gaggctctgg gatgatcatt ttttaaaaat 23100
cgaaggggtt ctggcgggaa tcagcatggc cagatggcag ggacacgttg cggaactgga 23160
acttgttctg ccacttgaac tcgggaatca ccagcctggg aactggaatc tctggaaagg 23220
tatcttgcca tagctttctg gtcagttgca gagcgccaag caggtcagga gcagatatct 23280
tgaaatcaca gttggggcca gaattctggg cgcgggagtt gcggtacact gggttgcagc 23340
actggaacac cataagggca gggtgtctca cgctcgccag cacggtctcg tcactgatgc 23400
aagacacatc caggtcttca gcattggcca ttccaaaggg ggtcatcttg caggtctgtc 23460
tgcccatcac gggagcgcag ccaggtttgt ggttgcaatc acaatgaagg gggatcagca 23520
tcatcttggc ctggtcgggg gtaatccctg ggtaaacagc cttcatgaag gcttcatact 23580
gcttgaaagc ttcctgggct ttggttccct cggtgtagaa cactccacaa gacttgctgg 23640
aaaactgatt agtagcgcag ttggcatcat tcacacagca gcgggcgtcg ttattagcca 23700
gctggaccac attcctgccc cagcggttct gggtgatctt ggctcgatct gggttctcct 23760
tcaacgcgcg ctggccgttc tcgctcgcca catccatctc aatgacatgt tccttctgga 23820
tcatgatgtt gccatgcagg catctaatct tgccttcata atcagtgcag ccatgaggcc 23880
acagcgcgca cccggtgcac tcccaattgt tatgggggat ctgggaatgg ctatgaacca 23940
gcccttgcag gaatcttccc atcatcacag ccagggtctt tatgctggta aaggtcagcg 24000
ggataccgcg gtgctcctcg ttcacatact gctggcagat gcgtctgtag tgctcggcct 24060
gctcgggcat cagcttgaaa gaggttttca actcattatc cagcctgtat ctctccatca 24120
tgatggacat tacttccatg cccttctccc aggcagaaac aatagggaga ctcaggggat 24180
tcttgacagt agagacaacc ttacttaagg ggtcatcact gccaatcttt tcgatgcttc 24240
tcttgccatc cttctcggtg atgcgcaccg gcgggtagct gaatcccaca gccaccaact 24300
gagcctcttc cctttcgtct tcgctgtctt gactgatgtc ttgcagagga acatgtttgg 24360
ttttcctggg tttcttcttg ggcggcagct ctggaggact ctggctccgt tccggagacc 24420
ccatggatga gcgagagttg tcgctcacca cttggatctg gctgcctgta gaagaactgg 24480
accccacgcg gcggtaggtg ttcctcttgg taggcagagg tggaggcgac gggctccggt 24540
ccggtctggg tggcggatgg ctggcggagc cccttccgcg ttcgggggtg cgctccagat 24600
ggcggtcgtc tgactgacct ccgcggctgg ccattgtgtt ctcctaggta gagaaacaag 24660
acatggagac tcagccatcg ctgccatcgc catccaccac cacaagcacc gccgaggagg 24720
aggagtgttt aaccacccca ccatgcagcc ccgctaccac caccagcacc cttgaaagcg 24780
aggtcgacac ggtcgtggag gatttacagg ctatggaaga tattgaggca gctgtcgagc 24840
aagaccccgg ctatgtgaca ccggcggagc atgatgagga tctagcgcgc tttctcgacg 24900
gtgtggagaa agcgaaacaa gatgaggacg aggaagaggc agaagcacaa ccatcggtgg 24960
ccgactacct caccggccta gggctagaag acgtgctgct taagcatctt gcaaggcaga 25020
cagtcatagt caaagacgcc ctgctagagc gctccgaggt gccactcagt gtggaagacc 25080
tcagtcgcgc ctatgagcta aacctcttct cgcctcgcaa gccccccaag cgtcagccca 25140
acgggacctg tgagcccaat ccgcgcctca acttctatcc agccttcact gtgcccgaag 25200
tactagctac ctaccacatc tttttcaaga accaaaagat ccccatctcc tgccgcgcca 25260
atcgcacccg cgcagatgcc ctactcaact tggggcccgg cgctcgcata cctgatatcg 25320
cttccttgga agaggttcct aagatctttg agggtctggg caatgaggaa actcgggcag 25380
caaacgctct gcaaagagaa acagatgatg gtgaacacca cagcgctctg gtggagctcc 25440
agggcgacaa cgctcgtctt gcagtcctca aacgcagcat cgaggtcacc catttcgcct 25500
accccgcact taatctccca cccaaagtca tgagctcggt catggacacg ttgctcatga 25560
agcgcgcgag ccccatctcc gaggatcaga acatgcagga ccccgatgcc tcagatgaag 25620
gcaagcctgt agtcagcgac gagcaactgg ctcgctggct aggctctgac tccccccagt 25680
ctttggagga gcggcgcaag cttatgatgg cagtggtcct gatcacagcg gagctggagt 25740
gtctccgccg cttcttcact gacccagaga ccctgcgcaa gcttgaggag aacctgcatt 25800
acacattcag tcatgggttc gtgcgccagg cgtgcaagat ctccaacgtt caactcacca 25860
acctggtctc ctacctgggc atcttgcatg aaaaccggct ggggcagaac gtgctccaca 25920
ccaccctgaa gggggaggcc cgccgcgact atatccgcga ctgtatctac ctctacctat 25980
gctacacctg gcaaagcggg atgggtgtgt ggcaacagtg cttggaagag caaaatctaa 26040
aagagctgga aaagctgctt cagaaatctc ttaaatctct gtggaccggg ttcgatgagc 26100
ggaccaccgc ttcggacatg gccgatatta tcttccccga gcggctcaga cacactctgc 26160
gcgacgggct gcctgacttt gccagccaga gcatgctaca aaactttagg tcattcatct 26220
tggaacgctc cgggatcctg cccgccactt gctgcgcact gccctccgat tttgtgccca 26280
tcacctaccg ggagtgcccc ccgccgctat ggagccactg ctacctgttc cgcctggcca 26340
actacttggc ctaccactct gatgtgatag aagatgttag tggcgaaggg ctcctggagt 26400
gccactgccg ctgcaacctc tgcacccccc accgctccct cgcctgcaat ccccagctgc 26460
tgagcgaaac ccagatcatc ggcaccttcg agttgcaagg tcccagcggc gaaggcgagg 26520
ggtcctctcc ggggcaaagt ctgaaactga ctccggggct atggacctcc gcttaccttc 26580
gcaagttcgc ccccaaagac taccacccct atgagatcag gttttatgaa gaccaatcac 26640
agccccccaa ggccgaactg acggcctgcg tcatcaccca gggggcaatc ttggcccaat 26700
tgcaagccat ccaaaaatcc cgccaagaat ttttgctgaa aaagggacac gggatctatc 26760
tagaccccca gaccggtgag gagctgaata cacgcttccc tcaggatgcc ccgaggaggc 26820
aagagaatga aagttcagat gccgcccgag gaggagctgg aagactggga cagtcaggca 26880
gaggaggaag actgggacag ccaggcagaa gaggaggaca gcctggagga ggacagtctg 26940
gaggaaggcg aggagcccaa ggaagaggca gccgccgcca gaccatcgtc ctcggcggtg 27000
gagacaagca aggtcccaga cagcacggct accacctccg ctccagctca aggggccgct 27060
cggcgaccca acagtagatg ggacgagacg ggtcgcttcc agaaccccac caccgtcaag 27120
accggtaagc aggagcggca gggatacaag tcctggcggg ggcataaaag tgccatcatc 27180
gcttgcttgc aggagtgtgg gggcaatata tcctttgcca gacgctacct gctattccat 27240
cacggggtga atttcccccg caacatcttg cattactacc gtcacctcca cagcccctac 27300
taccagcagc aagagacagc agaggaaacc agcggcaact ccgagagtta gaaaaccagc 27360
agctaaaaaa tccacagcgg cggcagcagg tgcaggcgga ctgaggatca ccgcgaacga 27420
gccagctcag accagggagt tgaggaatcg gatctttccc accctctatg ccatattcca 27480
acaaagtcgg ggtcaggaac aagaactgaa agtaaaaaac agatctcttc gctcgctcac 27540
ccgcagttgt ttgtatcaca agagcgaaga ccaacttcag cgcactctcg aggacgccga 27600
ggctctcttc aacaagtact gcgcgctgac tcttaaagag tagactgcgc gcgcttggcg 27660
agaaaaggcg ggaattacgt cacctcttgg ccacacctgt gcttcattat gagtaaagaa 27720
attcccacgc cttacatgtg gagctatcag ccccagatgg gattggccgc tggcgccgcc 27780
caggactact ccacccgcat gaattggctc agcgccggtc ccgcgatgat ctcacgggtt 27840
aatggtgtga gagagcaccg aaaccagata ctcctagaac agtccgccct caccgccact 27900
ccccgcaatc acctcaaccc ccgtaattgg cccgccgccc tggtgtacca ggaaactcct 27960
gctcccacta cagtactact tcctcgtgac gcccaggccg aagttcagat gactaactca 28020
ggtgtacagc tggcgggtgg tgccaccctg tgtcgtcacc ggccaagacc gggtataaag 28080
ggcctggtga tcagaggccg aggtattcag ctcaacgacg agtcggtgaa ctcttcgctt 28140
ggtctgcgac cagacggcat cttccaaata gctggttgtg ggagatcttc cttcactcct 28200
cgtcaggctg tcctgacttt ggagagttcg tcctcgcagc cccgctcggg cggcatcggg 28260
actctccagt tcgtggagga gtttactccc tcggtctact tcaacccctt ctccggttct 28320
cctgggcttt acccggacga gttcatcccg aactacgacg ccatcagtga agcggtcgac 28380
ggctacgatt aatgtctaat ggtggcgcgg ctgagctagc tcgactgcga cacctagacc 28440
actgccggcg ctttcgctgc tttgctcggg atctctgcga gttcatctac ttcgagtacc 28500
ctgacgaaca tcctcaggga cctgcccacg gagttcggat taccattgaa ggggctatcg 28560
attctcacct gcttcggatc ttcaccgctc ggccagtgct agttgagcgc aaccagggcg 28620
acaccaccat ctccctctgc tgcatttgtg acaaccccgg attgcatgaa agcttttgtt 28680
gtcttctttg tactgagtat aataaaagct gaaattagag actactccgg actctcttgt 28740
cgtctgaaca acaccaacca gacccttcac ttcagcggga accagactac tcttcactgt 28800
aaggcttata actataagta tcttacttgg atatacaaag gaacaccgtt tgctgtggta 28860
aacaggtgct ccaacgacgg tgttctcctc accttcctag gcaacttctc caactttacc 28920
ttttctgttc gcagaaacaa gcttaccctc cttcagccct actttcctgg gatctatacc 28980
tgcctcagtg gaccttgcaa ccacactttt cacctgattg aaaactctac ccttaccttc 29040
ccagcgccaa tccctactaa cagctcggag tccaactctt ccattaccgc tgatactaac 29100
actcctaaaa ccggaggtga gctccgcagc cttcccccgg ctgcagataa cccttgggtg 29160
gtagcgggat ttgtagcgct aggaatagtt gcgggtgggc tcgcgttcgt cctctgctac 29220
ctatacctta cctgctgctc atatttagta gtactgtgct gttggtttag aaaatggggg 29280
cgctactaat cacacttgct ttactttcgc ttttgggtct gagctcggct aatagcgaga 29340
aaccaagctg tctagaaaca aactctccag actgtgtggt tcctcatggg ctctcagacc 29400
cagctgatga tccatgctta acttttgacc cagaaaaaaa ctgctcggtg actatgcagc 29460
cctatgctta catgtgcaca tctgttataa agtgcggatg gggctgtaaa ccgattgaaa 29520
ttacccacaa aggcaaaacc tggaataata gtttgtttaa cacatggcag cctggagacg 29580
agcagtggta tacggccggc cactggtgga gatgactgac cccatggaaa actcctctgc 29640
caacgacctg gacatggacg gccgttcatc tgagcagcga ctggtccaga tgcgcattcg 29700
ccagaagcag gaacgcgccg ccagagagct caaggatgcc attgaaattc acctgtgcaa 29760
gaagggcatc ttttgcttgg ttaagcaagc aaagatttct tatgaaatca ctgacaacga 29820
ccaccgcctg tattatgagc tcggtccaca gcggcagaaa ttcacctgca tggttggagt 29880
caaccccata gtcatcactc agcaggctgc agaaattaaa gggtgcatcc actgttcctg 29940
tgattcccaa gaatgcgtcc acaccatagt caagaccctc tgcggccttc gagatcttct 30000
tccaatgaac taaccccttc ccccaaccca ataaaacatt ggttttaatc ataataaaaa 30060
atcacttact ttaaatctga aacagtgtct ccgtccaagt tttcttgtag caccacttca 30120
ctcccctctt cccagctctg gtactgcaag ccccggtggg ctgcaaactt tctccacacc 30180
ttaaaaggga tgtcaaattc ctcttgtcca acaatcttca ttgtctcttc ctagatgtcc 30240
acaaagcgcg cgcgggtgga agatgacttt gaccctgtct acccatacga tgctgagctg 30300
gcaccgtctg tacccttcat cgcccctccc ttcgtttcgt cagacggatt tcaagaaaaa 30360
cccctgggag ttctgtccct aagactagcc aacccagtca ctactaaaaa tggggaactc 30420
acacttaaac tgggagatgg ggtgggcata gactcagatg gaaacctcac agcacagaca 30480
gttactaaag caacatcccc ccttactgtt tccaataacg caattgcact taacatggac 30540
aaaccttttt acagtagcaa tggaaaacta tccttacaag ttacatcacc attaaagata 30600
gtcgactctt taaatacatt ggctattggc tatgggcaag gcttaggact aaacaatagt 30660
gctcttgctg tgcaattagc atctcccctt acatttgaca gcaacagcaa aattaaaata 30720
aatttgggaa gcgggccatt aaaaattaat gcgaataaac tgtcaattaa ctgcctaagg 30780
ggtgtatatg taacaactga cggaacttcc attgaaacaa atataagctg ggcaaaagga 30840
atgaggtttg aaggtaatgc catggctgta aacgttgaca gcaccaaagg tctacaattt 30900
ggcactacca gcacagaatc aggagtcact aacgctttcc ctatccagtt aaagattgga 30960
tctggtctta gttttgacag cacaggagca cttgtagctt gggataagga taatgacaag 31020
cttacactgt ggacaaccgc tgacccatca cctaattgta ccatatatac agacaaggat 31080
gctaaactta cactttgtct tacaaaatgt ggcagtcaaa tactaggcag tgtttcagta 31140
ctggctgtta aagctggaac cctacagcca atcagtgaaa aaataggtac tgctttggtt 31200
tcactaaaat ttaataacaa cggtgtattg ttaagcaact ccacattaag taatgaatac 31260
tggaactaca ggaagggaga tgtcacacca gccgaagcct atactaatgc tgtgggtttt 31320
atgccaaaca tcaaggcata tcctaaaaac acaaactctg cctcaaaaag ccacattgta 31380
ggacaagtgt accttaatgg agatgaaact aaaccaatgc atttaatcat tacatttaat 31440
gaaaccagtg atgaaacatg cacatattcc ataacgttcc aatggaaatg gaacattgga 31500
acatacacca gcgacaccct tgcaacaagc tcctttacct tttcttacat tgcccaagaa 31560
taaaaactgc agacaacaat aaagtttaaa tgttttattt aaacagtttc acagaaccct 31620
agtattcaac ctgccacctc cctcccaaca cacagagtac acagtccttt ctccccggct 31680
ggccttaaaa agcatcatat catgggtaac agacatattc ttaggtgtta tattccacac 31740
ggtttcctgt cgagccaaac gctcatcagt gatattaata aactccccgg gcagctcact 31800
taagttcatg tcgctgtcca gctgctgagc cacaggctgc tgtccaactt gcggttgctt 31860
aacgggcggc gaaggagaag tccacgccta catgggggta gagtcataat cgtgcatcag 31920
gatagggcgg tggtgctgca gcagcgcgcg aataaactgc tgccgccgcc gctccgtcct 31980
gcaggaatac aacatggcag tggtctcctc agcgatgatt cgcaccgccc gcagcataag 32040
gcgccttgtc ctccgggcac agcagcgcac cctgatctca cttaaatcag cacagtaact 32100
gcagcacagc accacaatat tgttcaaaat cccacagtgc aaggcgctgt atccaaagct 32160
catggcgggg accacagaac ccacgtggcc atcataccac aagcgcaggt agattaagtg 32220
gcgacccctc ataaacacgc tggacataaa cattacctct tttggcatgt tgtaattcac 32280
cacctcccgg taccatataa acctctgatt aaacatggcg ccatccacca ccatcctaaa 32340
ccagctggcc aaaacctgcc cgccggctat acactgcagg gaaccgggac tggaacaatg 32400
acagtggaga gcccaggact cgtaaccatg gatcatcatg ctcgtcatga tatcaatgtt 32460
ggcacaacac aggcacacgt gcatacactt cctcaggatt acaagctcct cccgcgttag 32520
aaccatatcc cagggaacaa cccattcctg aatcagcgta aatcccacac tgcagggaag 32580
acctcgcacg taactcacgt tgtgcattgt caaagtgtta cattcgggca gcagcggatg 32640
atcctccagt atggtagcgc gggtttctgt ctcaaaagga ggtagacgat ccctactgta 32700
cggagtgcgc cgagacaacc gagatcgtgt tggtcgtagt gtcatgccaa atggaacgcc 32760
ggacgtagtc attctcgtac tttgagtggc aaaaccttgc tctcgaacag cacacgtctc 32820
gtcgcctcct gtcccttctc ttcgcctttt cagtgtgata gttgtaatac agccattcac 32880
gaagctcagt cagaagatct tcagcgtctg ttgtcaaaaa caatccatcc aatctgattg 32940
ctttcaaaac atcacaaaca gtcgaataag ccaaacccat ccaggcaatg caattatttt 33000
ggttatccac aatgggaggg ggcggaagac atggaagagg cataattaat tttttaatcc 33060
aatcgatcac gcagcacttc aaaatgaaga tcgcgaaggt gacacctttc acccccactg 33120
tgttgatgaa aaataacagc caagtcaaaa ttgatgcggt tttcaaggtg ctcgactgta 33180
gcatcaagca gagcttccac acgcacgtcc acaaataaca gaatagcaaa agcgggagga 33240
ggaagtaaat cctcaatcat catagtacag tccatcacca tccctaaata attttcatcc 33300
ttccagcctt ggactatatt tttaaactgc tcttgtaaat ccaaaccaca catgtggaaa 33360
agttcccaaa gagctccctc aactaccatt cttaagcaca ccttcatagt gacaaaatat 33420
cttgttcctc tgtcacctgc agcaaattac aaagtccaat attaggatct atgcccagag 33480
atctaagctc atccctcaat tccaactgta aaaaggcttc cagatctgcc ctaacttgtt 33540
cagccagtgg gctccctgga ataagcgtgg gagaagccaa actgcaaaac agacgcatgc 33600
cgccataatt accaccagaa aacactacgt tacagtatgc atgctgattc attccagtaa 33660
tttcatccag tgtattggat acaaaaaaag gcaagcactc tctcactaat tgtattatgg 33720
agacattatc acacaggtaa caatttaaag gttgtggaac aataatgcag taagtaacca 33780
cggtgcgctc caacatggtt agtaattttt agttctgaaa aacaaaacat acaaaaaatt 33840
atatcatact catttggcga actggtggaa aaatgaccct atctagcaca aggcaagcca 33900
ctggatcacc aatgcgcccc tcataaaacc tgtcatcatg attaaaaagc aacaccgaaa 33960
gctcttccct atgtcctgca tgaatgattc tagctgagga atataagcca gcgcaattag 34020
tatctgttaa agaaaaaaaa cggccaacat agcctctagg aattagcaca cttaatctta 34080
aagacattac tgccatcccc cttggattta aggtaaaatt tacaggagca tagaaaatat 34140
actgatttcc ctcctgcaca ggcagcatag caccaggtcc ctctaaaaac acacacaaag 34200
cttctgcagc catagcttac cgcgcaaacc aggcacagca gtgagctaaa aggacaaagc 34260
tctaactcac tagccaacct ggcgcacaat atatagttag tccttacact gacgtaaccg 34320
accaaagtct aaaaaccccg ccaaaaatac acacacgccc aaaaaacgcc ccgtgagtca 34380
aaaaacagtt tcacttcctc gttacaccca aaacgtcgtc acttccggat tcccacggtt 34440
cgtcacttcc ggagctcctt gcttaattaa ccccgcccaa aacgtcatcg tccgcgtcac 34500
gccgccccgc cccgcgaccg ttgaccccgg gccaatcacc gcacatcccg caaaattcaa 34560
actcgtctaa tttgcatatt ggcacactgc ccatataagg tatattattg atgatg 34616

Claims (17)

1.一种分离的核酸序列，该分离的核酸序列编码六邻体多肽或其功能衍生物，该六邻体多肽或其功能衍生物包括具有SEQ ID NO:1的氨基酸序列或在其全长上与SEQ ID No:1的氨基酸序列具有至少95％同一性的氨基酸序列的含六邻体高变区多肽。
2.如权利要求1所述的分离的核酸序列，其中该六邻体多肽包括具有SEQ ID NO:1的氨基酸序列的含六邻体高变区多肽。
3.如权利要求2所述的分离的核酸序列，其中该六邻体多肽或其功能衍生物包含SEQID NO:2的氨基酸序列或在其全长上与SEQ ID NO:2的氨基酸序列具有至少95％同一性的氨基酸序列。
4.如权利要求1-3中任一项所述的分离的核酸序列，该分离的核酸序列进一步包括编码纤维多肽或其功能衍生物的核酸序列。
5.如权利要求4所述的分离的核酸序列，其中该纤维多肽包含选自由以下组成的组中的至少一种：(1)包含SEQ ID NO:10的氨基酸序列的纤维突结多肽序列，(2)包含SEQ IDNO:11的氨基酸序列的纤维轴多肽序列，和(3)包含SEQ ID NO:12的氨基酸序列的纤维尾多肽序列，优选地，该纤维多肽或其功能衍生物包含SEQ ID NO:3的氨基酸序列或在其全长上与SEQ ID NO:3的氨基酸序列具有至少95％同一性的氨基酸序列。
6.一种分离的核酸序列，该分离的核酸序列编码纤维多肽，该纤维多肽包含选自由以下组成的组中的至少一种：(1)包含SEQ ID NO:10的氨基酸序列的纤维突结多肽序列；(2)包含SEQ ID NO:11的氨基酸序列的纤维轴多肽序列，和(3)包含SEQ ID NO:12的氨基酸序列的纤维尾多肽序列。
7.如权利要求6所述的分离的核酸序列，其中该纤维多肽包含SEQ ID NO:3的氨基酸序列。
8.一种载体，该载体包含如权利要求1-7中任一项所述的核酸。
9.如权利要求8所述的载体，该载体为腺病毒载体，并且进一步包含转基因。
10.如权利要求9所述的腺病毒载体，其中该腺病毒载体进一步包含E1缺失和E3缺失中的至少一种。
11.如权利要求9或10所述的腺病毒载体，其中该腺病毒载体是包含一个或多个人腺病毒核酸序列的嵌合腺病毒载体，优选地，所述人腺病毒核酸序列来自人腺病毒-4、人腺病毒-5、人腺病毒-26或人腺病毒-35中的至少一种。
12.如权利要求9-11中任一项所述的腺病毒载体，其中该腺病毒载体包含选自由SEQID NO:13和SEQ ID NO:14组成的组的核酸序列。
13.一种重组细胞，该重组细胞包含如权利要求8-12中任一项所述的载体。
14.一种生产载体的方法，该方法包括：
(a)使如权利要求13所述的重组细胞在用于生产该载体的条件下生长；以及
(b)从该重组细胞中分离该载体。
15.一种免疫原性组合物，该免疫原性组合物包含如权利要求9-12中任一项所述的腺病毒载体。
16.一种在有需要的受试者中诱导免疫应答的方法，该方法包括向该受试者施用如权利要求15所述的免疫原性组合物。
17.一种生产疫苗的方法，该方法包括将如权利要求9-12中任一项所述的腺病毒载体与药学上可接受的载剂组合。
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