CN111295391B - 腺病毒及其用途 - Google Patents

腺病毒及其用途 Download PDF

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CN111295391B
CN111295391B CN201880070230.1A CN201880070230A CN111295391B CN 111295391 B CN111295391 B CN 111295391B CN 201880070230 A CN201880070230 A CN 201880070230A CN 111295391 B CN111295391 B CN 111295391B
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T·G·乌伊尔
S·罗伊
S·卡恩
J·H·H·V·屈斯泰
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Abstract

本文提供了腺病毒核酸序列和包含所述核酸序列的腺病毒载体。所提供的腺病毒载体可用于在受试者中诱导保护性免疫应答。

Description

腺病毒及其用途
技术领域
本发明涉及生物技术。更具体地说，涉及腺病毒载体诸如复制缺陷型腺病毒载体在宿主中递送抗原并引发免疫应答的领域和用途。
背景技术
重组腺病毒载体广泛应用于基因治疗应用和疫苗。基于AdV-5载体的疫苗已显示出在多种动物模型中引发强有力的和保护性的免疫应答(参见例如WO2001/02607；WO2002/22080；Shiver等人,Nature[自然]415:331(2002)；Letvin等人,Ann.Rev.Immunol.[免疫学年鉴]20:73(2002)；Shiver和Emini,Ann.Rev.Med.[医学年鉴]55:355(2004))。然而，基于重组AdV-5载体的疫苗的效用将可能受到人群中AdV-5特异性中和抗体(NAb)的高血清阳性率的限制。在小鼠、恒河猴和人的研究中，抗AdV-5免疫的存在已经显示出基本上抑制了基于AdV-5的疫苗的免疫原性。
一种在先前已被最常见的人腺病毒(例如AdV-5)感染或治疗的个体中规避预先存在的免疫性的有前景的策略涉及开发来自腺病毒血清型的重组载体，这些腺病毒血清型未遇到这种预先存在的免疫性。一种这样的策略是基于使用猿猴腺病毒，因为这些腺病毒通常不感染人并且在人样品中表现出低血清阳性率。猿猴腺病毒可应用于人类用途，因为已表明这些病毒可在体外感染人细胞(WO 2003/000283、WO 2004/037189)。
因此，本领域需要可大量生产的、在宿主中不会遇到预先存在的免疫、但仍具有免疫原性并能够诱导针对由插入载体中的异源核酸编码的抗原的强烈免疫应答的替代性腺病毒载体。
发明内容
已经分离了新型猿腺病毒，这些腺病毒在系统发育上与人腺病毒C种属于同一组。已经开发了含有新的猿腺病毒分离株的新型核酸序列的腺病毒载体以满足尚未满足的需要。提供了编码新型猿腺病毒分离株的六邻体多肽的分离的核酸序列。在某些实施例中，六邻体多肽包括具有SEQ ID NO:1的氨基酸序列的含六邻体高变区多肽。在某些实施例中，六邻体多肽包含BZ1六邻体多肽的氨基酸序列(SEQ ID NO:2)。
还提供了编码新型猿腺病毒分离株的纤维多肽的分离的核酸序列。在某些实施例中，纤维多肽包含BZ28纤维多肽的氨基酸序列(SEQ ID NO:3)。
本发明的实施例还包括由本发明的核酸序列编码的分离的纤维多肽和六邻体多肽。
本文进一步提供了分离的核酸，这些分离的核酸包含编码本文披露的六邻体多肽中的至少一种的核酸序列，以及编码本文披露的纤维多肽中的至少一种的核酸序列。在某些实施例中，本文提供了包含本文所述的分离的核酸的载体。在一个实施例中，该载体是病毒载体。在另一个实施例中，该载体是表达载体。在一个优选的实施例中，该载体是腺病毒载体。更优选地，该载体进一步包含至少一种转基因。
还提供了包含本文所述的载体的重组细胞。这样的细胞可以用于重组蛋白生产、重组蛋白表达，或载体或病毒颗粒的生产。还提供了生产载体的方法。这些方法包括(a)使本文披露的重组细胞在用于生产载体的条件下生长；以及(b)从该重组细胞中分离该载体。
在某些实施例中，提供了包含本文披露的载体的免疫原性组合物。还提供了在有需要的受试者中诱导免疫应答的方法，这些方法包括向该受试者施用本文披露的免疫原性组合物。
在某些实施例中，提供了腺病毒载体，这些腺病毒载体包含(a)至少一个转基因；以及(b)根据本发明的实施例的编码六邻体多肽的核酸序列。六邻体多肽可以例如包含具有SEQ ID NO:1的氨基酸序列的含六邻体高变区多肽。在某些实施例中，六邻体多肽包含BZ1六邻体多肽的氨基酸序列(SEQ ID NO:2)或BZ28六邻体多肽的氨基酸序列(SEQ ID NO:5)。
在某些实施例中，提供了腺病毒载体，这些腺病毒载体包含(a)至少一个转基因；以及(b)根据本发明的实施例的编码纤维多肽的核酸序列。在某些实施例中，纤维多肽包含选自BZ1(SEQ ID NO:4)或BZ28纤维多肽(SEQ ID NO:3)的氨基酸序列。本发明的实施例还包括腺病毒载体，这些腺病毒载体包含：(a)至少一个转基因；(b)根据本发明的实施例的编码六邻体多肽的核酸序列；以及(c)根据本发明的实施例的编码纤维多肽的核酸序列。
在某些实施例中，本文提供的腺病毒载体是复制缺陷型腺病毒载体(rAd)。在一个实施例中，腺病毒载体可以包含E1缺失。在某些实施例中，本文提供的腺病毒载体可进一步包含E3缺失。腺病毒载体可以是包含来自一种或多种猿猴腺病毒(SAdV)诸如黑猩猩腺病毒(例如ChAd3)、大猩猩腺病毒或恒河猴腺病毒(例如，rhAd51、rhAd52或rhAd53)的腺病毒核酸序列的猿猴腺病毒载体。腺病毒载体可以是包含来自一种或多种人腺病毒(例如，hAdV-4、hAdV-5、hAdV-26、hAdV-35)的腺病毒序列的人腺病毒载体。优选地，腺病毒载体是包含一个或多个人腺病毒核酸序列的嵌合腺病毒载体。人腺病毒核酸序列可以例如来自人腺病毒-4(hAdV-4)、人腺病毒-5(hAdV-5)、人腺病毒-26(hAdV-26)或人腺病毒-35(hAdV-35)。腺病毒载体可以例如包含人腺病毒-5(hAdV-5)E4orf6和orf6/7。
在某些实施例中，转基因与反向末端重复序列(ITR)相邻定位。在某些实施例中，转基因位于E1缺失处或附近、E3缺失处或附近、和/或右ITR(rITR)附近。
在某些实施例中，本文提供的腺病毒载体包含SEQ ID NO:9或SEQ ID NO:10的核酸序列。
还提供了包含本文所述的腺病毒载体和药学上可接受的载剂的免疫原性组合物或疫苗。进一步提供了用于在有需要的受试者中诱导免疫应答的方法。这些方法包括向该受试者施用本文披露的疫苗。进一步提供了生产疫苗的方法。这些方法包括将本文披露的腺病毒载体与药学上可接受的载剂组合。
附图说明
当结合附图阅读时，将更好地理解前述发明内容以及本申请的优选实施例的以下详细描述。然而，应当理解，本申请不限于附图中所示的精确实施例。
图1显示由BZ1/BZ28.FLuc诱导的细胞和体液免疫应答。图1A显示实验设置。图1B显示免疫后2周时由Ad26.FLuc和BZ1/BZ28.FLuc诱导的针对载体编码的插入物(即，FLuc，萤火虫萤光素酶)的细胞免疫应答，如通过干扰素γ(IFN-γ)ELISPOT分析所测定的。图1C显示用Ad26.FLuc、BZ1/BZ28.Fluc和空Ad26载体免疫后2周在小鼠中诱导的FLuc特异性IgG抗体滴度的图。
图2显示在用腺病毒载体Ad35、Ad26、Ad5、Ad4和BZ1/BZ28免疫的小鼠中诱导的同源和异源腺病毒中和滴度。
图3显示Ad26和BZ1/BZ28在200个来自年龄为18-55岁、生活在美国(US)和欧盟(EU)的成人的人类群组血清样品中的血清阳性率。将这些血清中针对每种载体测得的中和滴度分成四个类别(<16(无中和)、16至300、300至1,000、1000至4000和>4000)，如在所示的图表中表示的。
图4显示质粒pBZ1_BZ28F的示意图。
图5显示质粒pBZ1_BZ28F.5IXP的示意图。
图6显示新型载体BZ1/BZ28.Fluc在生产细胞系sPER.C6中的生产率。
具体实施方式
本披露至少部分地基于分离和鉴定分入人腺病毒C种的新的猿腺病毒血清型，以及构建和评价包含编码猿六邻体和纤维多肽的可变区的核酸的疫苗载体。另外，本披露至少部分地基于包含大猩猩腺病毒骨架和黑猩猩腺病毒六邻体序列的嵌合腺病毒载体的创建。这些腺病毒载体能够引发免疫应答，而且在人体中具有低血清阳性率。这些腺病毒载体可以被配制成疫苗并用于诱导针对感兴趣的特异性抗原的保护性免疫。
在背景技术和整个说明书中引用或描述了各种出版物、文章和专利；这些参考文献各自通过援引以其全文并入本文。包括在本说明书中的对文件、法案、材料、装置、制品等的讨论用于提供本发明的背景的目的。这种讨论不承认任何或所有这些事项形成关于所披露或要求保护的任何发明的现有技术的一部分。
除非另外定义，否则本文所用的所有科学和技术术语具有如本发明所属领域的普通技术人员通常理解的相同含义。否则，本文所用的某些术语具有说明书中阐述的含义。
必须注意，除非上下文另外明确规定，否则如本文和所附权利要求中所用，单数形式“一个/一种”和“该”包括复数指示物。
除非另有说明，否则任何数值，诸如本文所述的浓度或浓度范围，应被理解为在所有情况下都被术语“约”修饰。因此，数值典型地包括列举值±10％。例如，1mg/mL的浓度包括0.9mg/mL至1.1mg/mL。同样地，1％至10％(w/v)的浓度范围包括0.9％(w/v)至11％(w/v)。如本文所用，数值范围的使用明确地包括所有可能的子范围，该范围内的所有单个数值，包括值的这些范围内的整数和分数，除非上下文另有明确指示。
除非另外指明，否则在一系列要素前面的术语“至少”应被理解为指该系列中的每一个要素。本领域技术人员将认识到或能够仅使用常规实验便确定本文所述的本发明的具体实施例的许多等同方案。这些等同方案也旨在包括在本发明中。
如本文所用，术语“包括”、“包含”、“具有”、“含有”或它们的任何其他变化，将被理解为意味着包括所述整数或整数组，而不排除任何其他整数或整数组，并且旨在是非排他性的或开放性的。例如，包含元件的清单的组合物、混合物、工艺、方法、物品或设备不一定限制于仅那些元件，而可以包括不是此类组合物、混合物、工艺、方法、物品或设备明确列出的或固有的其他元件。此外，除非明确地说明是相反的，否则“或”是指包括在内的或，而不是指排他性的或。例如，以下项中的任一者满足条件A或B：A是真(或存在)并且B是假(或不存在)，A是假(或不存在)并且B是真(或存在)，以及A和B二者都是真(或存在)。
如本文所用，多个引用的要素之间的连接术语“和/或”应被理解为涵盖单独的和组合的选项两者。例如，当两种要素由和/或连接时，第一选项是指在不具有第二要素的情况下第一要素的应用。第二选项是指在不具有第一要素的情况下第二要素的应用。第三选项是指第一要素和第二要素一起的应用。这些选项中的任一个应被理解为属于该含义之内，并且因此满足如本文所用的术语“和/或”的要求。这些选项中的多于一个的同时应用也应被理解为属于该含义之内，并且因此满足术语“和/或”的要求。
如本文所用，贯穿说明书和权利要求书所用的术语“由……组成”指示包括任何列举的整数或整数组，但不能将另外的整数或整数组添加至指定的方法、结构或组合物。
如本文所用，贯穿说明书和权利要求书所用的术语“基本上由……组成”指示包括任何列举的整数或整数组，并且任选地包括不实质性改变所指定的方法、结构或组合物的基本或新颖特性的任何列举的整数或整数组。参见M.P.E.P.§2111.03。
如本文所用，“受试者”是指任何动物，优选哺乳动物，最优选人，其将或已经通过根据本发明实施例的方法接种。如本文所用的术语“哺乳动物”涵盖任何哺乳动物。哺乳动物的实例包括但不限于牛、马、羊、猪、猫、狗、小鼠、大鼠、兔、豚鼠、猴、人等，更优选地是人。
词语“右”、“左”、“下”和“上”表示所参考的附图中的方向。
应当理解的是，当提及优选的发明的组分的规格或特征时，在此使用的术语“约”、“大约”、“总体上”、“基本上”、以及类似术语，指示描述的规格/特征不具有严格的界限或参数，并且并不从其排除功能上相同或相似的微小变化，如本领域普通技术人员将理解的那样。在最低程度上，包括数值参数的此类引用将包括将不改变最低有效位数的变化，使用本领域接受的数学的和产业的原理(例如化整误差、测量误差或其他系统误差、制造公差等)。
在两个或更多个核酸或多肽序列(例如六邻体和纤维多肽以及编码它们的多核苷酸)的上下文中，术语“相同的”或“同一性”百分比是指当进行比较和比对以获得最大对应性时，两个或更多个序列或子序列是相同的或具有指定百分比的相同氨基酸残基或核苷酸，如使用以下序列比较算法之一或通过目视检查所测量的。
对于序列比较，典型地，一个序列作为参考序列，测试序列与其进行比较。当使用序列比较算法时，将测试序列和参考序列输入计算机；如果需要，指定子序列坐标；以及指定序列算法程序参数。然后，序列比较算法基于指定的程序参数计算测试序列相对于参考序列的序列同一性百分比。
用于比较的序列的最佳比对可以例如通过Smith&Waterman,Adv.Appl.Math.[应用数学进展]2:482(1981)的局部同源性算法、通过Needleman&Wunsch,J.Mol.Biol.[分子生物学杂志]48:443(1970)的同源性比对算法、通过Pearson&Lipman,Proc.Nat’l.Acad.Sci.USA[美国国家科学院院刊]85:2444(1988)的类似性搜索方法、通过这些算法的计算机化实现形式(GAP、BESTFIT、FASTA和TFASTA，威斯康星遗传学软件包(WisconsinGenetics Software Package),遗传学计算机课题组(Genetics Computer Group),威斯康星州麦迪逊科学大道575号(575Science Dr.,Madison,WI))、或通过目视检查(一般参见Current Protocols in Molecular Biology[分子生物学实验室指南],F.M.Ausubel等人编,Current Protocols[实验室指南],格林出版联合公司(Greene PublishingAssociates,Inc.)和约翰威利父子公司(John Wiley&Sons,Inc.)的合资公司,(1995年增刊)(Ausubel))来进行。
适于确定序列同一性百分比和序列相似性百分比的算法的实例是BLAST和BLAST2.0算法，这些算法描述于Altschul等人(1990)J.Mol.Biol.[分子生物学杂志]215:403-410和Altschul等人(1997)Nucleic Acids Res.[核酸研究]25:3389-3402。用于进行BLAST分析的软件可通过国家生物技术信息中心(National Center for BiotechnologyInformation)公开获得。该算法涉及首先通过鉴定查询序列中长度为W的短字来鉴定高得分序列对(HSP)，当与数据库序列中相同长度的字进行比对时，这些序列对匹配或满足一些正值阈值得分T。T被称为邻近字分值阈值(Altschul等人，同上)。这些初始的邻近字命中将作为种子，以用于启动搜索以发现包含它们的更长的HSP。然后，只要累积比对得分可以增加，字命中就沿着每个序列在两个方向上延伸。
对于核苷酸序列，累积得分使用参数M(一对匹配残基的奖励得分；总是>0)和N(错配残基的罚分；总是<0)来计算。对于氨基酸序列，使用评分矩阵来计算累积得分。当存在以下情况时，停止字命中在每个方向上的扩展：累积比对得分从其最大获得值下降数量X；由于一个或多个负得分残基比对的累积，累积得分变为零或更低；或者到达任一序列的末端。BLAST算法参数W、T和X决定比对的灵敏度和速度。BLASTN程序(用于核苷酸序列)使用11的字长(W)、10的期望值(E)、M＝5、N＝-4以及两条链的比较作为默认值。对于氨基酸序列，BLASTP程序使用3的字长(W)、10的期望值(E)和BLOSUM62评分矩阵作为默认值(参见Henikoff&Henikoff,Proc.Natl.Acad.Sci.USA[美国国家科学院院刊]89:10915(1989))。
除了计算序列同一性百分比之外，BLAST算法还进行两个序列之间相似性的统计学分析(参见例如Karlin&Altschul,Proc.Nat’l.Acad.Sci.USA[美国国家科学院院刊]90:5873-5787(1993))。BLAST算法提供的相似性的一种量度是最小和概率(P(N))，其提供两个核苷酸或氨基酸序列之间偶然发生匹配的概率的指示。例如，如果在测试核酸与参考核酸的比较中最小和概率小于约0.1，更优选小于约0.01，最优选小于约0.001，则认为该核酸与参考序列相似。
两个核酸序列或多肽基本上相同的另一个指示是由第一个核酸编码的多肽与由第二个核酸编码的多肽具有免疫交叉反应性，如下所述。因此，例如，如果某多肽和第二多肽仅因保守取代而不同，则两种肽通常基本上相同。两个核酸序列基本上相同的另一个指示是两个分子在严格条件下彼此杂交，如下所述。
如本文所用，术语“保护性免疫”或“保护性免疫应答”是指该接种疫苗的受试者能够控制对其进行了疫苗接种的致病因子的感染。致病因子可以是例如抗原基因产物或抗原蛋白，或其片段。通常，已经出现了“保护性免疫应答”的受试者仅出现轻度至中度临床症状或根本没有症状。通常，对某种因子具有“保护性免疫应答”或“保护性免疫”的受试者不会由于所述因子的感染而死亡。
术语“佐剂”定义为一种或多种引起免疫系统刺激的物质。在此上下文中，使用佐剂来增强对本发明的腺病毒载体的免疫应答。
如本文所用，术语“抗原基因产物或其片段”或“抗原蛋白”可包括细菌、病毒、寄生虫、或真菌蛋白、或其片段。优选地，抗原性蛋白或抗原性基因产物能够在宿主中产生保护性免疫应答，例如诱导针对疾病或感染(例如细菌、病毒、寄生虫或真菌疾病或感染)的免疫应答，和/或在受试者中产生针对疾病或感染的免疫(即，接种疫苗)，其保护受试者抵抗疾病或感染。
腺病毒载体
暴露于某些腺病毒已经导致针对某些腺病毒血清型的免疫应答，这可以影响腺病毒载体的功效。因为人腺病毒感染在人类中是常见的，所以在人群中抗人腺病毒的中和抗体的流行率高。预计个体中存在这种中和抗体会降低基于人腺病毒骨架的基因转移载体的功效。一种避免功效降低的方法是替换腺病毒衣壳蛋白上的表位，这些表位是中和抗体的靶标。衣壳蛋白上的靶序列可以用来自其他腺病毒的蛋白序列替换，这些腺病毒具有低流行性，并因此而言，针对这些腺病毒的中和抗体在人群中很少。
“衣壳蛋白”是指腺病毒衣壳上的蛋白(例如BZ1、BZ28、HAdV-4)或其功能片段或衍生物，其参与确定特定腺病毒的血清型和/或向性。衣壳蛋白典型地包括纤维蛋白、五邻体蛋白和/或六邻体蛋白。在某些实施例中，衣壳蛋白是腺病毒的完整或全长衣壳蛋白。在其他实施例中，衣壳蛋白是腺病毒的全长衣壳蛋白的片段或衍生物。在某些实施例中，由本发明的腺病毒载体编码的六邻体、五邻体和纤维具有相同或不同的腺病毒背景(即，BZ1六邻体和BZ28纤维、BZ1六邻体和BZ1纤维、BZ28六邻体和BZ1纤维、BZ28六邻体和BZ28纤维等)。
“六邻体多肽”指腺病毒六邻体外壳蛋白、其功能片段和衍生物。
“纤维多肽”指腺病毒纤维蛋白、其功能片段和衍生物。
针对腺病毒的中和抗体的一个靶标是主要外壳蛋白，即六邻体蛋白。用来自稀有腺病毒的六邻体蛋白替换六邻体蛋白，更优选地替换六邻体蛋白中定义血清型并结合中和抗体的可变序列，诸如从本文所述的猿猴中分离的那些，可以允许构建将不易被人类中常见的抗体中和的腺病毒载体。
针对腺病毒的中和抗体的第二个靶标是纤维蛋白。用来自非人源的稀有腺病毒的纤维序列替换纤维蛋白，更优选地替换纤维蛋白中的可变序列，诸如从本文所述的猿猴中分离的那些，也可以允许构建将不易被人类中常见的抗体中和的腺病毒载体。上述纤维替换与六邻体替换的组合可赋予对通常在人中发现的抗体的中和的额外抗性。
本公开提供了编码六邻体和/或纤维多肽的分离的核酸序列，这些六邻体和/或纤维多肽源自分离的猿猴腺病毒血清型；以及包含这些分离的核酸序列中的至少一种的腺病毒载体。
“腺病毒载体”指衍生自或包含腺病毒基因组的至少一部分的重组载体。
在优选的实施例中，分离的核酸序列编码六邻体多肽，这些六邻体多肽包括具有SEQ ID NO:1的氨基酸序列的含六邻体高变区多肽。在某些实施例中，六邻体多肽包含选自BZ1六邻体多肽(SEQ ID NO:2)或BZ28六邻体多肽(SEQ ID NO:5)的氨基酸序列。
在优选的实施例中，分离的核酸序列编码纤维多肽。纤维多肽可以例如包含选自BZ1纤维多肽(SEQ ID NO:4)或BZ28纤维多肽(SEQ ID NO:3)的氨基酸序列。
在优选的实施例中，提供了分离的核酸，其包含编码至少一种本文披露的六邻体多肽的核酸序列和编码至少一种本文披露的纤维多肽的核酸序列。
在优选的实施例中，提供了载体，优选腺病毒载体，其根据本发明的实施例包含编码六邻体多肽的分离的核酸序列和/或编码纤维多肽的分离的核酸序列中的至少一种。腺病毒载体可以例如包含BZ1六邻体多肽(SEQ ID NO:2)或BZ28六邻体多肽(SEQ ID NO:5)。腺病毒载体可以例如包含BZ1纤维多肽(SEQ ID NO:4)或BZ28纤维多肽(SEQ ID NO:3)。在某些实施例中，腺病毒载体包含编码六邻体多肽的核酸，其中该六邻体多肽包含选自BZ1六邻体多肽(SEQ ID NO:2)或BZ28六邻体多肽(SEQ ID NO:5)的氨基酸序列；以及编码纤维多肽的核酸，其中该纤维多肽包含选自BZ1纤维多肽(SEQ ID NO:4)或BZ28纤维多肽(SEQ IDNO:3)的氨基酸序列。腺病毒载体可以例如包含至少一个转基因；以及编码六邻体多肽和/或纤维多肽的核酸序列，其中六邻体多肽包括本文披露的含六邻体高变区多肽，并且纤维多肽包括本文所述的纤维多肽。
典型地，本发明的腺病毒载体包含在例如质粒、粘粒或杆状病毒载体上的完整重组腺病毒基因组。本发明的核酸分子可以呈RNA形式或呈通过克隆而获得或以合成方式而产生的DNA形式。DNA可以是双链的或单链的。
普通技术人员将认识到，衍生自多种血清型的元件可以组合在单个腺病毒载体中，例如人或猿猴腺病毒。因此，可以产生组合了来自不同血清型的期望性质的嵌合腺病毒载体。因此，在一些实施例中，本发明的嵌合腺病毒载体可以将不存在预先存在的猿猴六邻体和/或纤维多肽序列免疫性与现有腺病毒载诸如rAd4、rAd5、rAd26或rAd35的高水平抗原递送和呈递能力相结合。
用作疫苗的腺病毒载体的优点包括易于操纵、良好的大规模可制造性以及基于多年的研究、开发、制造和临床试验经验的优异安全记录，这些临床试验使用了许多已报道的腺病毒载体。被用作疫苗的腺病毒载体通常提供对转基因编码的蛋白的良好免疫应答，包括细胞免疫应答。根据本发明的腺病毒载体可以基于任何类型的腺病毒，并且在某些实施例中是人腺病毒，其可以属于任何组或血清型。在优选的实施例中，重组腺病毒基于来自A、B、C、D、E、F或G组的人腺病毒。在其他优选的实施例中，重组腺病毒基于人腺病毒血清型5、11、26、34、35、48、49或50。在其他实施例中，它是可以为任何血清型的猿猴腺病毒，诸如黑猩猩或大猩猩腺病毒。在某些实施例中，重组腺病毒基于黑猩猩腺病毒1、3、7、8、21、22、23、24、25、26、27.1、28.1、29、30、31.1、32、33、34、35.1、36、37.2、39、40.1、41.1、42.1、43、44、45、46、48、49、50、67型或SA7P。
在更优选的实施例中，第二组合物的黑猩猩腺病毒载体是ChAdV3。重组黑猩猩腺病毒血清型3(ChAd3或cAd3)是亚群C腺病毒，其性质类似于人腺病毒血清型5(Ad5)的性质。ChAd3在评估丙型肝炎病毒(HCV)候选疫苗的人类研究中已显示出是安全的和免疫原性的(Barnes E等人2012Science translational medicine[科学转化医学]4:115ra1)。据报道，基于ChAd3的疫苗能够诱导与人Ad5载体疫苗相当的免疫应答。参见例如Peruzzi D等人2009Vaccine[疫苗]27:1293-300和Quinn KM等人2013J Immunol[免疫学杂志]190:2720-35；WO 2005/071093；WO 2011/0130627等。
腺病毒载体、其构建方法及其繁殖方法是本领域所熟知的，并且描述于例如美国专利号5,559,099、5,837,511、5,846,782、5,851,806、5,994,106、5,994,128、5,965,541、5,981,225、6,040,174、6,020,191和6,113,913，以及Thomas Shenk,“Adenoviridae andtheir Replication”[腺病毒及其复制],M.S.Horwitz,“Adenoviruses”[腺病毒],分别第67和68章，见于Virology[病毒学],B.N.Fields等人编，第3版，纽约雷文出版社有限公司(Raven Press,Ltd.,New York)(1996)，以及本文提及的其他参考文献。典型地，腺病毒载体的构建涉及使用标准分子生物学技术，诸如以下文献中所述的那些技术：Sambrook等人,Molecular Cloning,a Laboratory Manual[分子克隆，实验室手册],第2版,纽约冷泉港的冷泉港出版社(Cold Spring Harbor Press,Cold Spring Harbor,N.Y.)(1989)；Watson等人,Recombinant DNA[重组DNA],第2版,Scientific American Books[科学美国人](1992)；和Ausubel等人,Current Protocols in Molecular Biology[分子生物学实验室指南],纽约威利国际科学出版公司(Wiley Interscience Publishers,NY)(1995)；以及本文提及的其他参考文献。
在某些实施例中，腺病毒载体包含E1缺失和/或E3缺失。E1或E3缺失可以例如包括基因的完全缺失或部分缺失，其使得E1或E3基因产物具有功能缺陷。因此，在某些实施例中，腺病毒是复制缺陷型的，例如因为它在基因组的E1区含有缺失。如技术人员已知的，在缺失来自腺病毒基因组的必需区域的情况下，由这些区域编码的功能必须优选的是由生产细胞反式提供，即，当E1、E2和/或E4区域的部分或全部从腺病毒中缺失时，这些区域必须存在于生产细胞中，例如整合到其基因组中，或呈所谓的辅助腺病毒或辅助质粒形式。这些腺病毒还可以具有在E3区域中的缺失，该缺失对于复制是非必要的，因此不必补充这种缺失。E1、E2、E3和E4区域中的一个或多个也可以通过其他方法失活，诸如通过将感兴趣的转基因(通常与启动子连接)插入待失活的区域。
可以使用的生产细胞(有时在本领域中以及在本文还称为‘包装细胞’或‘补充细胞’)可以是可在其中繁殖所希望的腺病毒的任何生产细胞。例如，在生产细胞中完成重组腺病毒载体的繁殖，这些生产细胞补充腺病毒中的缺陷。此类生产细胞优选地在其基因组中至少具有腺病毒E1序列，并因而能够补充在E1区域中具有缺失的重组腺病毒。可以使用任何补充E1的生产细胞，诸如由E1永生化的人类视网膜细胞，例如911或PER.C6细胞(参见美国专利5,994,128)、E1转化的羊水细胞(参见欧洲专利1230354)、E1转化的A549细胞(参见例如WO 98/39411、美国专利5,891,690)、GH329:HeLa(Gao等人,2000,Hum Gene Ther[人类基因治疗]11:213-19)、293等。在某些实施例中，生产细胞是例如HEK293细胞、或PER.C6细胞、或911细胞、或IT293SF细胞等。在(Kovesdi等人,2010,Viruses[病毒]2:1681-703)中对生产细胞中的腺病毒载体的生产进行了综述。
在某些实施例中，腺病毒载体是包含一个或多个人腺病毒核酸序列的嵌合腺病毒载体。人腺病毒核酸可以选自例如人腺病毒-4(Ad-4)、人腺病毒-5(Ad-5)、人腺病毒-26(Ad-26)或人腺病毒-35(Ad-35)。在某些实施例中，E1缺陷型腺病毒载体包含人Ad5的腺病毒的E4-orf6编码序列。这使得此类腺病毒可在表达Ad5的E1基因的熟知的补充细胞系中繁殖，诸如293细胞或PER.C6细胞(参见例如Fallaux等人,1998,Hum Gene Ther[人类基因治疗]9:1909-17；Havenga等人,2006,J Gen Virol[普通病毒学杂志]87:2135-43；WO 03/104467，它们通过援引以其全文并入本文)。
在某些实施例中，腺病毒载体包含转基因。“转基因”是指异源核酸，它是不天然存在于载体中的核酸，并且根据本发明，转基因可编码在受试者中引发免疫应答的抗原性基因产物或抗原性蛋白。转基因可以例如通过标准分子生物学技术引入载体中。例如，可将转基因克隆到腺病毒载体的缺失的E1或E3区域中，或E4区域与rITR之间的区域中。通常，转基因可操作地连接至表达控制序列。在优选的实施例中，转基因在转基因插入位点被插入。
如果需要，可以对根据本发明实施例的六邻体或纤维核酸序列、和/或转基因进行密码子优化，以确保在所治疗的宿主(例如人)中的正确表达。密码子优化是广泛应用于本领域的技术。
转基因可以在腺病毒衍生的启动子(例如，主要晚期启动子)的控制下(即，可操作地连接)，或者可以在异源启动子的控制下。合适的异源启动子的实例包括CMV启动子和RSV启动子。优选地，启动子位于表达盒内的感兴趣的异源基因的上游。
在优选的实施例中，腺病毒载体包含SEQ ID NO:9或SEQ ID NO:10的核酸序列。
免疫原性组合物
免疫原性组合物是用于本发明的包含免疫有效量的纯化的或部分纯化的人或猿猴(例如大猩猩)腺病毒载体的组合物。所述组合物可以根据本领域熟知的方法被配制成疫苗(也称为“免疫原性组合物”)。此类组合物可以包含佐剂以增强免疫应答。鉴于本披露，可以通过本领域技术人员熟知的技术确定制剂中每种组分的最佳比率。
鉴于本披露，根据本发明的实施例的免疫原性组合物可以使用本领域技术人员已知的方法制备。液体药物组合物通常包含液体载剂，诸如水、石油、动物或植物油、矿物油或合成油。可以包括生理盐水溶液、葡萄糖或其他糖溶液或二醇，诸如乙二醇、丙二醇或聚乙二醇。
可用于本发明的免疫原性组合物可以包含佐剂。适于根据本发明共同施用的佐剂应该是在人中潜在安全、耐受良好且有效的佐剂，包括QS-21、Detox-PC、MPL-SE、MoGM-CSF、TiterMax-G、CRL-1005、GERBU、TERamide、PSC97B、Adjumer、PG-026、GSK-I、AS01、AS03、AS04、AS15、GcMAF、B-alethine、MPC-026、Adjuvax、CpG ODN、Betafectin、Alum和MF59。
其他可以施用的佐剂包括凝集素、生长因子、细胞因子和淋巴因子，诸如α-干扰素、γ-干扰素、血小板衍生生长因子(PDGF)、粒细胞集落刺激因子(gCSF)、粒细胞巨噬细胞集落刺激因子(gMCSF)、肿瘤坏死因子(TNF)、表皮生长因子(EGF)、IL-I、IL-2、IL-4、IL-6、IL-8、IL-10和IL-12或其编码核酸。
本发明的组合物可以包含药学上可接受的赋形剂、载剂、缓冲剂、稳定剂或本领域技术人员熟知的其他材料。此类材料应该是无毒的，并且不应该干扰活性成分的功效。载剂或其他材料的确切性质可以取决于施用途径，例如肌内、皮下、经口、静脉内、皮肤、粘膜内(例如，肠)、鼻内或腹膜内途径。
诱导保护性免疫的方法
本发明的另一个一般方面涉及在需要的受试者中诱导免疫应答的方法。这些方法可以例如包括向受试者施用包含本文所述的腺病毒载体和药学上可接受的载剂的疫苗。本文还提供了生产疫苗的方法。这些方法包括将本文所述的腺病毒载体与药学上可接受的载剂组合。
根据本发明的实施例的任何免疫原性组合物(包括但不限于本文所述的那些)均可在本发明方法中用作疫苗。
包含载体的免疫原性组合物/疫苗的施用典型地是肌内或皮下。然而，也可以设想其他施用方式，诸如静脉内、皮肤、皮内或鼻。免疫原性组合物的肌内施用可以通过使用针注射腺病毒载体的混悬液来实现。一种替代方案是使用无针注射装置来施用组合物(使用例如BiojectorTM)或含有疫苗的冷冻干燥粉末。
对于静脉内、皮肤或皮下注射，或在病痛部位的注射，载体将呈肠胃外可接受的水溶液的形式，它是无热原的并具有合适的pH、等渗性和稳定性。本领域技术人员完全能够使用例如等渗媒介物诸如氯化钠注射液、林格氏注射液、乳酸盐林格氏注射液来制备适合的溶液。根据需要，可以包括防腐剂、稳定剂、缓冲剂、抗氧化剂和/或其他添加剂。还可以使用缓释制剂。
典型地，施用将具有预防性目的，以在感染或发展症状之前产生针对感兴趣的抗原(例如，细菌、病毒、寄生虫和/或真菌病原体)的免疫应答。可根据本发明治疗或预防的疾病和病症包括免疫应答可在其中起到保护或治疗作用的那些。在其他实施例中，腺病毒载体可被施用用于暴露后预防。
将含有人或猿猴(例如大猩猩)腺病毒载体的免疫原性组合物施用给受试者，从而在受试者中产生对感兴趣的抗原的免疫应答。足以诱导可检测的免疫应答的组合物的量被定义为组合物的“免疫有效剂量”或“有效量”。本发明的免疫原性组合物可以诱导体液以及细胞介导的免疫应答。在典型的实施例中，免疫应答是保护性免疫应答。
施用的实际量以及施用的速率和时程将取决于正治疗的疾病的性质和严重度。治疗处方(例如剂量等的决定)是在全科医生和其他医师、或在兽医环境中是兽医的职责范围内的，并且典型地考虑了待治疗的病症、个体患者的状况、递送部位、施用方法以及医师已知的其他因素。以上提及的这些技术和方案的实例可以在Remington's PharmaceuticalSciences[雷明顿药学大全],第16版，Osol,A.编,1980中找到。
在产生腺病毒载体和任选将此类颗粒配制成组合物后，可以将这些载体施用给个体，特别是人或其他灵长类动物。可以对人或另一种哺乳动物施用，例如小鼠、大鼠、仓鼠、豚鼠、兔、绵羊、山羊、猪、马、牛、驴、猴、狗或猫。向非人哺乳动物的递送不必是为了治疗目的，而是可以用于实验环境，例如研究对腺病毒载体的免疫应答机制。
在一个示例性方案中，腺病毒载体以约100μl至约10ml范围内的体积施用(例如肌内)，该体积的浓度为约104至1012个病毒颗粒/ml。优选地，腺病毒载体以0.1-2.0ml范围内的体积施用。例如，腺病毒载体可以100μl、500μl、1ml、2ml施用。更优选地，腺病毒载体以0.5ml的体积施用。任选地，腺病毒载体以107vp/ml、108vp/ml、109vp/ml、1010vp/ml、5x1010vp/ml、1011vp/ml或1012vp/ml的浓度施用。典型地，在一次施用期间，腺病毒载体以约109至约1012个病毒颗粒(vp)的量，更典型地以约1010至约1012个vp的量施用于人受试者。
初始疫苗接种之后可以是从包含编码感兴趣抗原的相同腺病毒载体的疫苗/组合物或包含编码相同感兴趣抗原的不同腺病毒载体的疫苗/组合物的加强或激发。
如果需要，组合物可以存在于试剂盒、包装或分配器中，该试剂盒、包装或分配器可以包含一个或多个含有活性成分的单位剂型。例如，试剂盒可以包括金属或塑料箔，诸如泡罩包装。试剂盒、包装或分配器可附有施用说明书。
本发明的组合物可以单独施用或与其他治疗联合施用(同时地或按顺序)，这取决于待治疗的病状。
实施例
本发明还提供了以下非限制性实施例。
实施例1是一种分离的核酸序列，该分离的核酸序列编码六邻体多肽，该六邻体多肽包括具有SEQ ID NO:1的氨基酸序列的含六邻体高变区多肽。
实施例2是如实施例1所述的分离的核酸序列，其中该六邻体多肽包含BZ1六邻体多肽的氨基酸序列(SEQ ID NO:2)。
实施例3是如实施例1或2所述的分离的核酸序列，该分离的核酸序列进一步包含编码纤维多肽的核酸序列。
实施例4是如实施例3所述的分离的核酸序列，其中该纤维多肽包含选自BZ1纤维多肽(SEQ ID NO:4)或BZ28纤维多肽(SEQ ID NO:3)的氨基酸序列。
实施例5是一种分离的核酸序列，该分离的核酸序列编码包含BZ28纤维多肽的氨基酸序列(SEQ ID NO:3)的纤维多肽。
实施例6是一种载体，该载体包含如实施例1-5中任一项所述的核酸。
实施例7是如实施例6所述的载体，该载体是腺病毒载体，并且进一步包含至少一个转基因。
实施例8是一种重组细胞，该重组细胞包含如实施例6或7所述的载体。
实施例9是一种生产载体的方法，该方法包括：(a)使如实施例8所述的重组细胞在用于生产该载体的条件下生长；以及(b)从该重组细胞中分离该载体。
实施例10是一种免疫原性组合物，该免疫原性组合物包含如实施例7所述的载体。
实施例11是一种在有需要的受试者中诱导免疫应答的方法，该方法包括向该受试者施用如实施例10所述的免疫原性组合物。
实施例12是一种腺病毒载体，该腺病毒载体包含(a)至少一个转基因；以及(b)编码六邻体多肽的核酸序列，该六邻体多肽包括具有SEQ ID NO:1的氨基酸序列的含六邻体高变区多肽。
实施例13是如实施例12所述的腺病毒载体，其中该六邻体多肽包含BZ1六邻体多肽的氨基酸序列(SEQ ID NO:2)。
实施例14是如实施例12或13所述的腺病毒载体，该腺病毒载体进一步包含编码纤维多肽的核酸序列。
实施例15是如实施例14所述的腺病毒载体，其中该纤维多肽包含选自BZ1纤维多肽(SEQ ID NO:4)或BZ28纤维多肽(SEQ ID NO:3)的氨基酸序列。
实施例16是一种腺病毒载体，该腺病毒载体包含(a)至少一个转基因；以及(b)编码纤维多肽的核酸序列，该纤维多肽包含BZ28纤维多肽的氨基酸序列(SEQ ID NO:3)。
实施例17是一种腺病毒载体，该腺病毒载体包含(a)至少一个转基因；(b)编码六邻体多肽的核酸序列，该六邻体多肽包含选自BZ1六邻体多肽(SEQ IDNO:2)或BZ28六邻体多肽(SEQ ID NO:5)的氨基酸序列；以及(c)编码纤维多肽的核酸序列，该纤维多肽包含选自BZ1纤维多肽(SEQ ID NO:4)或BZ28纤维多肽(SEQ ID NO:3)的氨基酸序列。
实施例18是如实施例12-17中任一项所述的腺病毒载体，其中该腺病毒载体进一步包含E1缺失。
实施例19是如实施例12-18中任一项所述的腺病毒载体，其中该腺病毒载体进一步包含E3缺失。
实施例20是如实施例12-19中任一项所述的腺病毒载体，其中该腺病毒载体是包含一个或多个人腺病毒核酸序列的嵌合腺病毒载体。
实施例21是如实施例20所述的腺病毒载体，其中该人腺病毒核酸序列来自人腺病毒-4、人腺病毒-5、人腺病毒-26或人腺病毒-35。
实施例22是如实施例21所述的腺病毒载体，其中该腺病毒载体包含人腺病毒-5(HAdv-5)E4 orf6。
实施例23是如实施例12-22中任一项所述的腺病毒载体，其中该转基因与反向末端重复序列(ITR)相邻。
实施例24是如实施例23所述的腺病毒载体，其中转基因位于E1缺失处或附近、E3缺失处或附近和/或ITR附近。
实施例25是如实施例12-24中任一项所述的腺病毒载体，其中该腺病毒载体包含选自SEQ ID NO:9或SEQ ID NO:10的核酸序列。
实施例26是一种疫苗，该疫苗包含如实施例12-25中任一项所述的腺病毒载体和药学上可接受的载剂。
实施例27是一种在有需要的受试者中诱导免疫应答的方法，该方法包括向该受试者施用如实施例26所述的疫苗。
实施例28是一种生产疫苗的方法，该方法包括将如实施例12-25中任一项所述的腺病毒载体与药学上可接受的载剂组合。
实施例29是一种分离的六邻体多肽，该分离的六邻体多肽包括具有SEQ IDNO:1的氨基酸序列的含六邻体高变区多肽。
实施例30是如实施例29所述的分离的六邻体多肽，其中该六邻体多肽包含BZ1六邻体多肽的氨基酸序列(SEQ ID NO:2)。
实施例31是一种分离的纤维多肽，其中该纤维多肽包含BZ28纤维多肽的氨基酸序列(SEQ ID NO:3)。
实例
实例1：基于新型腺病毒分离株BZ1的骨架并包含新型腺病毒分离株BZ28的纤维序列的E1和E3缺失型载体的生成
鉴定了新的大猩猩腺病毒分离株BZ1(也称为JAd4-WT)，并对其基因组进行了完整测序。还鉴定了新的黑猩猩分离株BZ28(也称为JAd5-WT)，并对其纤维基因及其一部分六邻体基因进行了测序。发现新的腺病毒分离株BZ1和BZ28均在系统发育上属于人腺病毒C种(HAdV-C)。确定BZ1的全基因组核苷酸序列为SEQ ID NO:6。确定BZ28的纤维和(部分)六邻体核苷酸序列分别为SEQ ID NO:3和SEQ ID NO:5。
用于生成基于BZ1/BZ28的腺病毒载体的单质粒系统的描述
pBZ1_BZ28F(SEQ ID NO:9；图4)和pBZ1_BZ28F.5IXP(SEQ ID NO:10；图5)是各自携带全长、E1和E3缺失型基于BZ1分离株的腺病毒载体基因组的质粒，其中纤维编码序列被BZ28分离株的序列替换。这些质粒中包含的基于BZ1/BZ28的腺病毒载体基因组序列分别在SEQ ID NO:11和SEQ ID NO:12中列出。在这些质粒的每一个中，腺病毒载体基因组的侧翼是两个SwaI限制性酶切位点(即一个SwaI位点位于载体基因组的任一端)。这些SwaI位点是为了在病毒拯救之前通过转染合适的E1补充细胞(例如HEK293、911和PER.C6细胞)来促进从质粒骨架上切除腺病毒载体基因组。这些质粒所包含的腺病毒载体基因组进一步携带某些限制性酶切位点，这些限制性酶切位点在E1缺失的位置中以及在右反向末端重复序列(RITR)附近被引入。在完整的腺病毒基因组质粒的情况下，将这些限制性酶切位点选择为独特的。它们代表“转基因插入位点”，这些位点允许通过标准分子克隆技术容易地构建携带一个或多个转基因表达盒的腺病毒载体，该一个或多个转基因表达盒插入在所述相应位置中的任一个处或其任何组合中。腺病毒载体设计和质粒构建在以下章节中更详细地描述。
基于BZ1/BZ28的腺病毒载体基因组设计
BZ1/BZ28和BZ1/BZ28.5IXP腺病毒载体基因组(即，分别为SEQ ID NO:11和SEQID:12)各自设计为包含E1缺失、E3缺失、不同的转基因插入位点以及天然E4开放阅读框(orf)6和orf6/7被人腺病毒-5(HAdV-5)的开放阅读框替换。使每种腺病毒的E1区域缺失，并用包含AsiSI限制性酶切位点序列的转基因插入位点替换。通过在每个腺病毒的反向末端重复序列(ITR)附近插入PacI限制性酶切位点序列，产生另一个转基因插入位点。腺病毒载体基因组进一步包含(BZ1)E4 orf6和orf6/7编码区序列被人腺病毒-5(HAdV-5)的相应序列替换。SEQ ID NO:7所示的HAdV-5替换序列由GenBank序列AC_000008的核苷酸32914-34077组成。
设计和构建了两种类型的E1区域缺失。pBZ1_BZ28F所包含的基于BZ1/BZ28的腺病毒载体基因组携带对应于SEQ ID NO:6的核苷酸491至3324的移除的E1区域缺失。相比之下，pBZ1_BZ28F.5IXP所包含的基于BZ1/BZ28的腺病毒载体基因组携带较大的包含E1区域的序列缺失，从而移除了BZ1的所有E1编码序列(即，SEQ ID NO:6的核苷酸491至3484)。此外，该后一个腺病毒载体基因组还被设计成携带E1B 55K与pIX编码序列之间的非编码序列段被HAdV-5的非编码序列段替换(即，对应于SEQ ID NO:6的核苷酸3485至3574的序列被GenBank AC_000008的核苷酸3510至3608替换(即，被SEQ ID NO:8替换))。
包含基于BZ1/BZ28的腺病毒载体基因组的单质粒的构建
通过若干基因合成步骤(由金斯瑞公司执行)和标准分子克隆程序构建了pBZ1_BZ28F(SEQ ID NO:9)。首先，进行几个连续的基因合成和亚克隆步骤，它们一起导致pBR322(GenBank登录号-J01749.1)的2.3-kbp EcoRI-NdeI片段被8.1-kbp的合成核苷酸序列替换，该合成核苷酸序列包含SwaI限制性位点侧接序列，该侧接序列包含设计的BZ1/BZ28腺病毒载体基因组的左端部分和右端部分，其中所述左端部分包含上述E1缺失，并且所述右端部分包含上述E3缺失、BZ28纤维替换、HAdV-5E4 orf6和orf6/7替换、以及与rITR相邻的PacI限制性位点插入(在右端部分内)。所得质粒即BZ1/BZ28中间质粒1的核苷酸序列在SEQID NO:13中列出。其次，将BZ1病毒基因组的12.3-kbp NheI-AscI限制性片段(SEQ ID NO:6)连接到NheI和AscI消化的BZ1/BZ28中间质粒1中，从而产生BZ1/BZ28中间质粒2。第三，将BZ1病毒基因组的11.8-kbp NdeI-HpaI限制性片段连接到NdeI和HpaI消化的BZ1/BZ28中间质粒2中，从而产生BZ1/BZ28中间质粒3。第四，将BZ1病毒基因组的3.3-kbp NdeI-NdeI限制性片段连接到NdeI消化的BZ1/BZ28中间质粒3中，从而产生最终的质粒pBZ1_BZ28F(SEQ IDNO:9)。
pBZ1_BZ28F.5IXP(SEQ ID NO:10)通过以下标准基因合成和分子克隆程序构建。首先，合成由修饰的BZ1/BZ28载体基因组片段组成的2927bp DNA片段(SEQ ID NO:14)，该载体基因组片段包含HAdV-5的某些pIX启动子序列(即，SEQ ID NO:8所示的那些)，并作为AsiSI-SalI限制性片段连接到AsiSI和SalI消化的BZ1/BZ28中间质粒2中，从而产生BZ1/BZ28.5IXP中间质粒1。第二，将BZ1病毒基因组的15.2-kbp SalI-SalI限制性片段(SEQ IDNO:6)连接到SalI消化的BZ1/BZ28.5IXP中间质粒1中，从而产生BZ1/BZ28.5IXP中间质粒2。第三，将BZ1病毒基因组的10.0-kbp ClaI-SpeI限制性片段连接到ClaI和SpeI消化的BZ1.BZ28.5IXP中间质粒2中，从而产生最终的质粒pBZ1_BZ28F.5IXP(SEQ ID NO:10)。
pBZ1_BZ28F.Fluc(SEQ ID NO:15)和pBZ1_BZ28F.RSVF-2A-GLuc(SEQ ID NO:16)是pBZ1_BZ28F衍生的质粒，它们各自带有基于BZ1/BZ28的腺病毒载体基因组，该腺病毒载体基因组装备有在E1缺失位置插入的转基因表达盒。这些质粒内携带的腺病毒载体基因组序列分别在SEQ ID NO:17和SEQ ID NO:18中列出。pBZ1_BZ28F.Fluc携带萤火虫萤光素酶(FLuc)的转基因表达盒。该盒由巨细胞病毒主要立即早期启动子(即“CMV启动子”)驱动，并含有SV40衍生的聚腺苷酸化信号。pBZ1_BZ28F.RSVF-2A-GLuc携带“RSV-FA2-2A-GLuc”的转基因表达盒(RSVF-2A-GLuc)，它是由呼吸道合胞病毒株A2融合糖蛋白、口蹄疫病毒2A肽和高斯(Gaussia)萤光素酶(GLuc)构成的嵌合蛋白。像Fluc盒一样，该盒也由CMV启动子驱动，并携带SV40聚腺苷酸化信号。另外，该盒在其5’非翻译区中包含含有人载脂蛋白A1基因的内含子2的序列。将Fluc和RSVF-2A-GLuc表达盒各自通过若干标准基因合成和分子克隆步骤构建，然后将它们连接到pBZ1_BZ28F的独特AsiSI限制性酶切位点中，从而分别生成pBZ1_BZ28F.Fluc和pBZ1_BZ28F.RSVF-2A-GLuc。
基于BZ1/BZ28的腺病毒载体的生成和生产
通过将相应的腺病毒载体基因组质粒(即，pBZ1_BZ28F.Fluc和pBZ1_BZ28F.RSVF-2A-GLuc)转染到补充E1的PER.C6细胞中，生成了分别包含腺病毒载体基因组序列SEQ IDNO:17和SEQ ID NO:18的腺病毒载体BZ1/BZ28.Fluc(也称为JAd4C5NVT003)和BZ1/BZ28.RSVF-2A-Gluc(也称为JAd4C5NVT001)。在转染到PER.C6细胞(这些细胞在补充了10％胎牛血清(FBS)和10mM MgCl2的达尔伯克氏改良伊格尔培养基(DMEM)中作为贴壁细胞培养物生长)中之前，用SwaI消化腺病毒载体基因组质粒以从质粒释放相应的腺病毒载体基因组。使用Lipofectamine转染试剂(加利福利亚州卡尔斯巴德的英杰公司(Invitrogen；Carlsbad,CA))，根据标准程序进行转染。在收获病毒拯救转染后，通过在PER.C6细胞培养物上的连续几轮感染而进一步扩增病毒。如前所述(Havenga等人,“Novel replication-incompetent adenoviral B-group vectors:high vector stability and yield inPER.C6 cells[新的无复制能力的腺病毒B组载体：在PER.C6细胞中的高载体稳定性和产率]”J.Gen.Virol.[普通病毒学杂志]87(8):2135-43(2006))，使用两步氯化铯(CsCl)密度梯度超速离心程序从粗病毒收获物中纯化病毒。病毒颗粒(VP)滴度通过先前描述的基于分光光度法的程序进行测量(Maizel等人,“The polypeptides of adenovirus:I.Evidencefor multiple protein components in the virion and a comparison of types 2,7A,and 12,”[腺病毒的多肽：I.病毒体中多种蛋白组分的证据以及2、7A和12型的比较]Virology[病毒学],36(1):115-25(1968))。
实例2：由BZ1/BZ28.FLuc诱导的细胞和体液免疫应答
使用萤火虫萤光素酶(FLuc)作为载体编码的(模型)疫苗抗原，评估了新型腺病毒载体BZ1/BZ28的细胞和体液免疫原性。用Ad26.FLuc(阳性对照)或BZ1/BZ28.FLuc(均以每只小鼠109和1010个病毒颗粒)或用不编码转基因的阴性对照载体(空Ad26，每只小鼠1010个病毒颗粒)对Balb/C小鼠进行肌内免疫。在两周后处死小鼠并收集血样和脾细胞(图1A)。
通过FLuc特异性ELISPOT试验评估了针对载体编码的抗原的细胞免疫诱导。为此，将分离的脾细胞用跨越FLuc蛋白的15mer重叠肽刺激过夜，并通过离体ELISPOT试验测定细胞免疫应答，该试验测量IFN-γ分泌细胞的相对数量(图1B)。结果表明，在较高剂量免疫(1010)下，由BZ1/BZ28诱导的细胞免疫应答大约与Ad26.FLuc所见的应答一样高。相比之下，在较低剂量免疫(109)下，BZ1/BZ28.FLuc比Ad26.FLuc产生了强得多的应答。
BZ1/BZ28诱导针对其编码的抗原的体液免疫的能力通过测量针对FLuc所产生的抗体来评估。在FLuc特异性IgG抗体ELISA中测试来自免疫小鼠的血清(在2周时收集)。图1C显示，在每只小鼠1010vp下，表达萤火虫萤光素酶的BZ1/BZ28载体引发的终点滴度高于Ad26.FLuc基准。正如预期的那样，在用不编码萤火虫萤光素酶的空载体免疫的小鼠血清中未检测到Fluc特异性抗体滴度。
总体而言，数据显示BZ1/BZ28载体可诱导针对载体编码的抗原的强有力的细胞和体液免疫应答，其大小高于基于HAdV-26的基准载体所诱导的应答。这些免疫应答清楚地表明了BZ1/BZ28载体在小鼠中的强有力的免疫原性。
实例3：对新型和现有腺病毒载体之间的血清交叉中和的评估
由于它们作为新腺病毒疫苗载体的潜在用途，本文产生的新型BZ1/BZ28腺病毒载体优选地在血清学上不同于目前已经开发作为疫苗载体(诸如基于人腺病毒血清型HAdV-5和HAdV-35的载体)的现有腺病毒载体。因此，在新型BZ1/BZ28腺病毒载体以及基于HAdV-4、HAdV-5、HAdV-26和HAdV-35的几种现有载体之间进行了交叉中和测试。为此，在腺病毒中和试验中，针对每种不同的载体测试小鼠抗血清，每种抗血清针对这些腺病毒载体之一而产生。在将Balb/C小鼠用每只小鼠1010个载体颗粒免疫后两周或八周时，从这些小鼠收集用于该试验的小鼠抗血清。如先前所述(Spangers等人2003.J.Clin.Microbiol.[临床微生物学杂志]41:5046-5052)进行腺病毒中和试验。简而言之，从1:16稀释开始，将血清进行2倍系列稀释，然后与表达萤火虫萤光素酶(FLuc)的腺病毒载体预混合，然后与A549细胞温育过夜(以每个细胞500个病毒颗粒的感染复数)。感染后24小时测得的被感染细胞裂解物中的萤光素酶活性水平代表载体感染效率。针对给定载体的中和滴度定义为能够使载体感染效率降低90％的最高血清稀释度。中和滴度可任意分为以下几类：<16(无中和)、16至200、200至2,000和>2,000。
结果表明，所测试的载体之间没有交叉中和作用(图2)。BZ1/BZ28显示出同源中和抗体应答，但未显示出与测试组中包括的人腺病毒载体即Ad26、Ad35、Ad5和Ad4的交叉中和。因此，该新腺病毒载体BZ1/BZ28可以潜在地与一种或多种这些或其他不同的腺病毒载体联合用于顺序免疫，例如在异源初免-加强疫苗接种方案的情况下，或者，可替代地或另外地，在一系列针对不同疾病或抗原的两个或更多个连续疫苗接种方案的情况下。
实例4：新型腺病毒载体的人血清阳性率
对于它们作为有效疫苗载体的潜在用途而言重要的是，本文所述的新型腺病毒载体不受疫苗目标群体中高水平的预先存在的抗载体体液免疫的妨碍。因此，在200个来自成年人的人群血清样品中评价了BZ1/BZ28载体的血清阳性率，这些成年人年龄为18-55岁，生活在美国(US)和欧盟(EU)。通过如先前所述的(Sprangers等人2003.J.Clin.Microbiol.[临床微生物学杂志]41:5046-5052)标准腺病毒中和试验，测试了载体被人血清样品中和的情况。简而言之，从1:16稀释开始，将血清进行2倍系列稀释，然后与表达萤火虫萤光素酶(FLuc)的腺病毒载体预混合，然后与A549细胞温育过夜(以每个细胞500个病毒颗粒的感染复数)。感染后24小时测得的被感染细胞裂解物中的萤光素酶活性水平代表载体感染效率。针对给定载体的中和滴度定义为能够使载体感染效率降低90％的最高血清稀释度。中和滴度可任意分为以下几类：<16(无中和)、16至300、300至1000、1000至4000和>4000。结果表明BZ1/BZ28在所研究的人类受试者中的血清阳性率与Ad5相似(图3)。但是，与Ad5相比，针对新型BZ1/BZ28载体所见的阳性中和滴度通常很低。例如，在所有测试的血清中，超过25％显示出>1000的Ad5中和滴度，而只有约3％具有该水平的BZ1/BZ28中和滴度。
总体而言，上述数据表明，就预先存在的载体中和滴度水平而言，在所评估的疫苗目标群体中，可以认为针对BZ1/BZ28载体的预先存在的体液抗载体免疫相对较低，这表明这些载体在这些群体中具有作为有效疫苗载体的潜力。
实例5：在悬浮PER.C6细胞中的腺病毒载体生产率
用于临床试验及其他方面的腺病毒载体需要在可扩展的无血清腺病毒生产平台中容易地生产至高滴度。悬浮-适应的PER.细胞(本文也称为悬浮PER.C6细胞或sPER.C6)代表了这样的平台，因为它们已经显示出支持在生物反应器中大规模生产腺病毒载体，获得大量高滴度的临床级载体制备物，例如基于HAdV-26或HAdV-35的E1缺失型载体的制备物(EP 2536829B1、EP 2350268B1)。
作为关于本文所述的新型载体是否适合基于sPER.C6细胞的生产工艺的初步评估，对在摇瓶中培养的sPER.C6细胞进行了小规模载体生产率实验。这些生产率实验使用实例1中所述的新型腺病毒载体BZ1/BZ28的Fluc编码形式进行。基于HAdV-26的载体Ad26.Fluc用作基准对照。将以1×106个细胞/ml的密度接种在摇瓶中的总体积为10ml的补充了4mM L-谷氨酰胺(龙沙集团(Lonza))的培养基(可从龙沙集团获得)中的悬浮PER.C6细胞培养物用不同的载体以不同的病毒颗粒(VP)-细胞比感染，然后温育4天。用于感染的不同VP-细胞比是70、150和900。每天采集被感染的细胞培养物的样品，并通过基于定量PCR(qPCR)的方案测定这些样品中的VP滴度，该方案采用对CMV启动子(其存在于所有测试的载体中)特异的引物和探针。该方案需要在qPCR之前对测试样品进行DNA酶处理，以除去任何游离的载体DNA(即未包装到病毒颗粒中的载体基因组)。
新型载体BZ1/BZ28.Fluc所得的生产率结果如图6所示。在所测试的所有VP-细胞感染比和收获时间点下，BZ1/BZ28.Fluc显示出比基准对照载体Ad26.Fluc更高的VP滴度。这些结果证明了新型BZ1/BZ28载体在基于sPER.C6的无血清悬浮细胞培养模型上的良好生产率。
总之，如上所述，对由本发明的新型重组基于BZ1/BZ28的腺病毒载体诱导的体液和细胞免疫应答进行的研究清楚地表明这些载体在小鼠中的强有力的免疫原性。另外，这些载体证实不诱导针对某些现有腺病毒疫苗载体候选物(例如Ad26、Ad35和Ad5)的交叉中和抗体应答，反之亦然。此外，当与Ad5相比时，新载体在人体中显示出相对较低的预先存在的载体中和滴度水平。最后，这些新载体可以容易地以高产率产生。低血清阳性率、强有力的免疫原性和可生产性的组合表明本发明的新型腺病毒载体可用作针对多种病原体的新型疫苗载体候选物，并且可另外用于基因疗法和/或诊断。
本领域技术人员将理解，在不偏离本发明的广泛发明构思的情况下，可以对上述实施例进行改变。因此，应当理解，本发明不限于所披露的特定实施例，而是旨在涵盖由本说明书限定的本发明的精神和范围内的修改。
序列表
<110> 扬森疫苗与预防公司
<120> 腺病毒及其用途
<130> 0299 WO 00 ORD
<160> 19
<170> PatentIn 3.5版
<210> 1
<211> 321
<212> PRT
<213> 人工序列
<220>
<223> BZ1六邻体HVR
<400> 1
Glu Thr Gln Asn Glu Val Gln Ala Asn Glu Glu Gln Leu Ala Glu Glu
1 5 10 15
Glu Asp Glu Glu Met Ala Gln Glu Asp Gln Gln Pro Thr Lys Lys Thr
20 25 30
His Val Tyr Ala Gln Ala Pro Leu Ser Gly Glu Gln Ile Thr Lys Asp
35 40 45
Gly Leu Gln Ile Gly Ala Glu Val Thr Gly Glu Thr Ser Lys Pro Ile
50 55 60
Phe Ala Asp Lys Thr Phe Gln Pro Glu Pro Gln Ile Gly Glu Ser Gln
65 70 75 80
Trp Asn Glu Ala Asp Ala Thr Val Ala Gly Gly Arg Val Leu Lys Lys
85 90 95
Thr Thr Pro Met Lys Pro Cys Tyr Gly Ser Tyr Ala Arg Pro Thr Asn
100 105 110
Ala Asn Gly Gly Gln Gly Ile Leu Glu Ala Asn Ala Lys Gly Glu Leu
115 120 125
Glu Ser Lys Val Glu Met Gln Phe Phe Ser Asn Thr Thr Thr Leu Asn
130 135 140
Val Arg Asp Gly Glu Asn Gly Leu Lys Pro Lys Val Val Leu Tyr Ser
145 150 155 160
Glu Asp Val Asn Leu Glu Ser Pro Asp Thr His Leu Ser Tyr Lys Pro
165 170 175
Lys Lys Asp Asp Val Asn Ala Lys Ile Met Leu Gly Gln Gln Ala Met
180 185 190
Pro Asn Arg Pro Asn Leu Ile Gly Phe Arg Asp Asn Phe Ile Gly Leu
195 200 205
Met Tyr Tyr Asn Ser Thr Gly Asn Met Gly Val Leu Ala Gly Gln Ala
210 215 220
Ser Gln Leu Asn Ala Val Val Asp Leu Gln Asp Arg Asn Thr Glu Leu
225 230 235 240
Ser Tyr Gln Leu Met Leu Asp Ser Ile Gly Asp Arg Thr Arg Tyr Phe
245 250 255
Ser Met Trp Asn Gln Ala Val Asp Ser Tyr Asp Pro Asp Val Arg Ile
260 265 270
Ile Glu Asn His Gly Val Glu Asp Glu Leu Pro Asn Tyr Cys Phe Pro
275 280 285
Leu Gly Gly Ile Gly Ile Thr Asp Thr Tyr Gln Ala Ile Lys Ala Ala
290 295 300
Asn Gly Gly Asp Ala Thr Thr Trp Ser Ala Asp Asn Thr Phe Ala Asp
305 310 315 320
Arg
<210> 2
<211> 961
<212> PRT
<213> 人工序列
<220>
<223> BZ1六邻体
<400> 2
Met Ala Thr Pro Ser Met Met Pro Gln Trp Ser Tyr Met His Ile Ser
1 5 10 15
Gly Gln Asp Ala Ser Glu Tyr Leu Ser Pro Gly Leu Val Gln Phe Ala
20 25 30
Arg Ala Thr Asp Thr Tyr Phe Asn Met Ser Asn Lys Phe Arg Asn Pro
35 40 45
Thr Val Ala Pro Thr His Asp Val Thr Thr Asp Arg Ser Gln Arg Leu
50 55 60
Thr Leu Arg Phe Ile Pro Val Asp Arg Glu Asp Thr Ala Tyr Ser Tyr
65 70 75 80
Lys Ala Arg Phe Thr Leu Ala Val Gly Asp Asn Arg Val Leu Asp Met
85 90 95
Ala Ser Thr Tyr Phe Asp Ile Arg Gly Val Leu Asp Arg Gly Pro Thr
100 105 110
Phe Lys Pro Tyr Ser Gly Thr Ala Tyr Asn Ser Leu Ala Pro Lys Gly
115 120 125
Ala Pro Asn Ser Cys Glu Trp Glu Glu Glu Thr Gln Asn Glu Val Gln
130 135 140
Ala Asn Glu Glu Gln Leu Ala Glu Glu Glu Asp Glu Glu Met Ala Gln
145 150 155 160
Glu Asp Gln Gln Pro Thr Lys Lys Thr His Val Tyr Ala Gln Ala Pro
165 170 175
Leu Ser Gly Glu Gln Ile Thr Lys Asp Gly Leu Gln Ile Gly Ala Glu
180 185 190
Val Thr Gly Glu Thr Ser Lys Pro Ile Phe Ala Asp Lys Thr Phe Gln
195 200 205
Pro Glu Pro Gln Ile Gly Glu Ser Gln Trp Asn Glu Ala Asp Ala Thr
210 215 220
Val Ala Gly Gly Arg Val Leu Lys Lys Thr Thr Pro Met Lys Pro Cys
225 230 235 240
Tyr Gly Ser Tyr Ala Arg Pro Thr Asn Ala Asn Gly Gly Gln Gly Ile
245 250 255
Leu Glu Ala Asn Ala Lys Gly Glu Leu Glu Ser Lys Val Glu Met Gln
260 265 270
Phe Phe Ser Asn Thr Thr Thr Leu Asn Val Arg Asp Gly Glu Asn Gly
275 280 285
Leu Lys Pro Lys Val Val Leu Tyr Ser Glu Asp Val Asn Leu Glu Ser
290 295 300
Pro Asp Thr His Leu Ser Tyr Lys Pro Lys Lys Asp Asp Val Asn Ala
305 310 315 320
Lys Ile Met Leu Gly Gln Gln Ala Met Pro Asn Arg Pro Asn Leu Ile
325 330 335
Gly Phe Arg Asp Asn Phe Ile Gly Leu Met Tyr Tyr Asn Ser Thr Gly
340 345 350
Asn Met Gly Val Leu Ala Gly Gln Ala Ser Gln Leu Asn Ala Val Val
355 360 365
Asp Leu Gln Asp Arg Asn Thr Glu Leu Ser Tyr Gln Leu Met Leu Asp
370 375 380
Ser Ile Gly Asp Arg Thr Arg Tyr Phe Ser Met Trp Asn Gln Ala Val
385 390 395 400
Asp Ser Tyr Asp Pro Asp Val Arg Ile Ile Glu Asn His Gly Val Glu
405 410 415
Asp Glu Leu Pro Asn Tyr Cys Phe Pro Leu Gly Gly Ile Gly Ile Thr
420 425 430
Asp Thr Tyr Gln Ala Ile Lys Ala Ala Asn Gly Gly Asp Ala Thr Thr
435 440 445
Trp Ser Ala Asp Asn Thr Phe Ala Asp Arg Asn Glu Ile Gly Val Gly
450 455 460
Asn Asn Phe Ala Met Glu Ile Asn Ile Gln Ala Asn Leu Trp Arg Asn
465 470 475 480
Phe Leu Tyr Ala Asn Val Gly Leu Tyr Leu Pro Asp Lys Leu Lys Tyr
485 490 495
Asn Pro Thr Asn Val Asp Ile Ser Asp Asn Pro Asn Thr Tyr Asp Tyr
500 505 510
Met Asn Lys Arg Val Val Ala Pro Gly Leu Val Asp Cys Phe Val Asn
515 520 525
Val Gly Ala Arg Trp Ser Leu Asp Tyr Met Asp Asn Val Asn Pro Phe
530 535 540
Asn His His Arg Asn Ala Gly Leu Arg Tyr Arg Ser Met Ile Leu Gly
545 550 555 560
Asn Gly Arg Tyr Val Pro Phe His Ile Gln Val Pro Gln Lys Phe Phe
565 570 575
Ala Ile Lys Asn Leu Leu Leu Leu Pro Gly Ser Tyr Thr Tyr Glu Trp
580 585 590
Asn Phe Arg Lys Asp Val Asn Met Val Leu Gln Ser Ser Leu Gly Asn
595 600 605
Asp Leu Arg Val Asp Gly Ala Ser Ile Lys Phe Asp Ser Ile Thr Leu
610 615 620
Tyr Ala Thr Phe Phe Pro Met Ala His Asn Thr Ala Ser Thr Leu Glu
625 630 635 640
Ala Met Leu Arg Asn Asp Thr Asn Asp Gln Ser Phe Asn Asp Tyr Leu
645 650 655
Ser Gly Ala Asn Met Leu Tyr Pro Ile Pro Ala Lys Ala Thr Asn Val
660 665 670
Pro Ile Ser Ile Pro Ser Arg Asn Trp Ala Ala Phe Arg Gly Trp Ala
675 680 685
Phe Thr Arg Leu Lys Thr Lys Glu Thr Pro Ser Leu Gly Ser Gly Phe
690 695 700
Asp Pro Tyr Phe Val Tyr Ser Gly Ser Ile Pro Tyr Leu Asp Gly Thr
705 710 715 720
Phe Tyr Leu Asn His Thr Phe Lys Lys Ile Ser Ile Met Tyr Asp Ser
725 730 735
Ser Val Ser Trp Pro Gly Asn Asp Arg Leu Leu Thr Pro Asn Glu Phe
740 745 750
Glu Val Lys Arg Ala Val Asp Gly Glu Gly Tyr Asn Val Ala Gln Cys
755 760 765
Asn Met Thr Lys Asp Trp Phe Leu Val Gln Met Leu Ala Asn Tyr Asn
770 775 780
Ile Gly Tyr Gln Gly Phe Tyr Ile Pro Glu Ser Tyr Lys Asp Arg Met
785 790 795 800
Tyr Ser Phe Phe Arg Asn Phe Gln Pro Met Ser Arg Gln Val Val Asp
805 810 815
Glu Thr Asn Tyr Lys Asp Tyr Gln Ala Ile Gly Ile Thr His Gln His
820 825 830
Asn Asn Ser Gly Phe Val Gly Tyr Leu Ala Pro Thr Met Arg Glu Gly
835 840 845
Gln Ala Tyr Pro Ala Asn Phe Pro Tyr Pro Leu Ile Gly Lys Thr Ala
850 855 860
Val Asp Ser Val Thr Gln Lys Lys Phe Leu Cys Asp Arg Thr Leu Trp
865 870 875 880
Arg Ile Pro Phe Ser Ser Asn Phe Met Ser Met Gly Ala Leu Thr Asp
885 890 895
Leu Gly Gln Asn Leu Leu Tyr Ala Asn Ser Ala His Ala Leu Asp Met
900 905 910
Thr Phe Glu Val Asp Pro Met Asp Glu Pro Thr Leu Leu Tyr Ile Val
915 920 925
Phe Glu Val Phe Asp Val Val Arg Val His Gln Pro His Arg Gly Val
930 935 940
Ile Glu Thr Val Tyr Leu Arg Thr Pro Phe Ser Ala Gly Asn Ala Thr
945 950 955 960
Thr
<210> 3
<211> 543
<212> PRT
<213> 人工序列
<220>
<223> BZ28纤维
<400> 3
Met Lys Arg Thr Lys Thr Ser Asp Glu Ser Phe Asn Pro Val Tyr Pro
1 5 10 15
Tyr Asp Thr Glu Asn Gly Pro Pro Ser Val Pro Phe Leu Thr Pro Pro
20 25 30
Phe Val Ser Pro Asp Gly Phe Gln Glu Ser Pro Pro Gly Val Leu Ser
35 40 45
Leu Asn Leu Ala Glu Pro Leu Val Thr Ser His Gly Met Leu Ala Leu
50 55 60
Lys Met Gly Ser Gly Leu Ser Leu Asp Asp Ala Gly Asn Leu Thr Ser
65 70 75 80
Gln Asp Val Thr Thr Thr Thr Pro Pro Leu Lys Lys Thr Lys Thr Asn
85 90 95
Leu Ser Leu Glu Thr Ser Ala Pro Leu Thr Val Ser Thr Ser Gly Ala
100 105 110
Leu Thr Leu Ala Ala Ala Val Pro Leu Ala Val Ala Gly Thr Ser Leu
115 120 125
Thr Met Gln Ser Glu Ala Pro Leu Thr Val Gln Asp Ala Lys Leu Thr
130 135 140
Leu Ala Thr Lys Gly Pro Leu Thr Val Ser Glu Gly Lys Leu Ala Leu
145 150 155 160
Gln Thr Ser Ala Pro Leu Thr Ala Ala Asp Ser Ser Ala Leu Thr Val
165 170 175
Ser Ala Thr Pro Pro Ile Ser Val Ser Ser Gly Ser Leu Gly Leu Asp
180 185 190
Met Glu Asp Pro Met Tyr Thr His Asp Gly Lys Leu Gly Ile Arg Ile
195 200 205
Gly Gly Pro Leu Arg Val Val Asp Ser Leu His Thr Leu Thr Val Val
210 215 220
Thr Gly Asn Gly Ile Ala Val Asp Asn Asn Ala Leu Gln Thr Arg Val
225 230 235 240
Thr Gly Ala Leu Gly Tyr Asp Thr Ser Gly Asn Leu Gln Leu Arg Ala
245 250 255
Ala Gly Gly Met Arg Ile Asp Ala Asn Gly Gln Leu Ile Leu Asp Val
260 265 270
Ala Tyr Pro Phe Asp Ala Gln Asn Asn Leu Ser Leu Arg Leu Gly Gln
275 280 285
Gly Pro Leu Tyr Val Asn Thr Asp His Asn Leu Asp Leu Asn Cys Asn
290 295 300
Arg Gly Leu Thr Thr Thr Thr Thr Asn Asn Thr Lys Lys Leu Glu Thr
305 310 315 320
Lys Ile Gly Ser Gly Leu Asp Tyr Asp Thr Asn Gly Ala Val Ile Ile
325 330 335
Lys Leu Gly Thr Gly Val Ser Phe Asp Ser Thr Gly Ala Leu Thr Val
340 345 350
Gly Asn Thr Gly Asp Asp Lys Leu Thr Leu Trp Thr Thr Pro Asp Pro
355 360 365
Ser Pro Asn Cys Arg Ile His Ala Asp Lys Asp Cys Lys Phe Thr Leu
370 375 380
Val Leu Thr Lys Cys Gly Ser Gln Ile Leu Ala Ser Val Ala Ala Leu
385 390 395 400
Ala Val Ser Gly Asn Leu Ala Ser Ile Thr Gly Thr Val Ala Ser Val
405 410 415
Thr Ile Phe Leu Arg Phe Asp Gln Asn Gly Val Leu Met Glu Asn Ser
420 425 430
Ser Leu Asp Lys Gln Tyr Trp Asn Phe Arg Asn Gly Asn Ser Thr Asn
435 440 445
Ala Thr Pro Tyr Thr Asn Ala Val Gly Phe Met Pro Asn Leu Ala Ala
450 455 460
Tyr Pro Lys Thr Gln Ser Gln Thr Ala Lys Asn Asn Ile Val Ser Gln
465 470 475 480
Val Tyr Leu Asn Gly Asp Lys Ser Lys Pro Met Thr Leu Thr Ile Thr
485 490 495
Leu Asn Gly Thr Asn Glu Ser Ser Glu Thr Ser Gln Val Ser His Tyr
500 505 510
Ser Met Ser Phe Thr Trp Ala Trp Glu Ser Gly Gln Tyr Ala Thr Glu
515 520 525
Thr Phe Ala Thr Asn Ser Phe Thr Phe Ser Tyr Ile Ala Glu Gln
530 535 540
<210> 4
<211> 580
<212> PRT
<213> 人工序列
<220>
<223> BZ1纤维
<400> 4
Met Lys Arg Ala Arg Ser Ser Asp Glu Thr Phe Asn Pro Val Tyr Pro
1 5 10 15
Tyr Asp Thr Glu Ile Ala Pro Thr Ser Val Pro Phe Leu Thr Pro Pro
20 25 30
Phe Val Ser Ser Ala Gly Met Gln Glu Asn Pro Ala Gly Val Leu Ser
35 40 45
Leu His Leu Ser Glu Pro Leu Thr Thr His Asn Gly Ala Leu Thr Leu
50 55 60
Lys Met Gly Gly Gly Leu Thr Leu Asp Lys Glu Gly Asn Leu Thr Ser
65 70 75 80
Gln Asn Ile Thr Ser Val Asp Pro Pro Leu Lys Lys Ser Lys Asn Asn
85 90 95
Ile Ser Leu Gln Thr Ala Ala Pro Leu Ala Val Ser Ser Gly Ala Leu
100 105 110
Thr Leu Phe Ala Thr Pro Pro Leu Ala Val Ser Gly Asp Asn Leu Thr
115 120 125
Val Gln Ser Gln Ala Pro Phe Thr Leu Glu Asp Ser Lys Leu Thr Leu
130 135 140
Ala Thr Lys Gly Pro Leu Thr Val Ser Glu Gly Lys Leu Val Leu Glu
145 150 155 160
Thr Glu Ala Pro Leu His Ala Ser Asp Ser Ser Ser Leu Gly Leu Ser
165 170 175
Val Thr Ala Pro Leu Ser Ile Asn Asn Asp Ser Leu Gly Leu Asp Met
180 185 190
Gln Ala Pro Ile Ser Ser Arg Asp Gly Lys Leu Ala Leu Thr Val Ala
195 200 205
Ala Pro Leu Thr Val Ala Glu Gly Ile Asn Ala Leu Ala Val Ala Thr
210 215 220
Gly Asn Gly Ile Gly Leu Asn Glu Thr Asn Thr His Leu Gln Ala Lys
225 230 235 240
Leu Val Ala Pro Leu Gly Phe Asp Thr Asn Gly Asn Ile Lys Leu Ser
245 250 255
Val Ala Gly Gly Met Arg Leu Asn Asn Asn Thr Leu Ile Leu Asp Val
260 265 270
Asn Tyr Pro Phe Glu Ala Gln Gly Gln Leu Ser Leu Arg Val Gly Ser
275 280 285
Gly Pro Leu Tyr Val Asp Ser Ser Ser His Asn Leu Thr Ile Arg Cys
290 295 300
Leu Arg Gly Leu Tyr Val Thr Ser Ser Asn Asn Gln Asn Gly Leu Glu
305 310 315 320
Ala Asn Ile Lys Leu Thr Lys Gly Leu Val Tyr Asp Gly Asn Ala Ile
325 330 335
Ala Val Asn Val Gly Lys Gly Leu Glu Tyr Ser Pro Thr Gly Thr Thr
340 345 350
Glu Lys Pro Ile Gln Thr Lys Ile Gly Leu Gly Met Glu Tyr Asp Thr
355 360 365
Glu Gly Ala Met Met Thr Lys Leu Gly Ser Gly Leu Ser Phe Asp Asn
370 375 380
Ser Gly Ala Ile Val Val Gly Asn Lys Asn Asp Asp Arg Leu Thr Leu
385 390 395 400
Trp Thr Thr Pro Asp Pro Ser Pro Asn Cys Gln Ile Tyr Ser Glu Lys
405 410 415
Asp Ala Lys Leu Thr Leu Val Leu Thr Lys Cys Gly Ser Gln Val Val
420 425 430
Gly Thr Val Ser Ile Ala Ala Leu Lys Gly Ser Leu Val Pro Ile Thr
435 440 445
Ser Ala Ile Ser Val Val Gln Val Tyr Leu Arg Phe Asp Glu Asn Gly
450 455 460
Val Leu Met Ser Asn Ser Ser Leu Asn Gly Glu Tyr Trp Asn Phe Arg
465 470 475 480
Asn Gly Asp Ser Thr Asn Gly Thr Pro Tyr Thr Asn Ala Val Gly Phe
485 490 495
Met Pro Asn Leu Leu Ala Tyr Pro Lys Gly Gln Thr Thr Thr Ala Lys
500 505 510
Ser Asn Ile Val Ser Gln Val Tyr Met Asn Gly Asp Asp Thr Lys Pro
515 520 525
Met Thr Phe Thr Ile Asn Phe Asn Gly Leu Ser Glu Thr Gly Asp Thr
530 535 540
Pro Val Ser Lys Tyr Ser Met Thr Phe Ser Trp Arg Trp Pro Asn Gly
545 550 555 560
Ser Tyr Ile Gly His Asn Phe Val Thr Asn Ser Phe Thr Phe Ser Tyr
565 570 575
Ile Ala Gln Glu
580
<210> 5
<211> 579
<212> PRT
<213> 人工序列
<220>
<223> BZ28部分六邻体
<400> 5
Lys Phe Arg Asn Pro Thr Val Ala Pro Thr His Asp Val Thr Thr Asp
1 5 10 15
Arg Ser Gln Arg Leu Thr Leu Arg Phe Ile Pro Val Asp Arg Glu Asp
20 25 30
Thr Ala Tyr Ser Tyr Lys Ala Arg Phe Thr Leu Ala Val Gly Asp Asn
35 40 45
Arg Val Leu Asp Met Ala Ser Thr Tyr Phe Asp Ile Arg Gly Val Leu
50 55 60
Asp Arg Gly Pro Thr Phe Lys Pro Tyr Ser Gly Thr Ala Tyr Asn Ser
65 70 75 80
Leu Ala Pro Lys Gly Ala Pro Asn Ser Cys Glu Trp Glu Gln Glu Glu
85 90 95
Thr Gln Ala Val Glu Glu Ala Ala Glu Glu Glu Glu Glu Asp Ala Asp
100 105 110
Gly Gln Ala Glu Glu Glu Gln Ala Ala Thr Lys Lys Thr His Val Tyr
115 120 125
Ala Gln Ala Pro Leu Ser Gly Glu Lys Ile Ser Lys Asp Gly Leu Gln
130 135 140
Ile Gly Thr Asp Ala Thr Ala Thr Glu Gln Lys Pro Ile Tyr Ala Asp
145 150 155 160
Pro Thr Phe Gln Pro Glu Pro Gln Ile Gly Glu Ser Gln Trp Asn Glu
165 170 175
Ala Asp Ala Thr Val Ala Gly Gly Arg Val Leu Lys Lys Ser Thr Pro
180 185 190
Met Lys Pro Cys Tyr Gly Ser Tyr Ala Arg Pro Thr Asn Ala Asn Gly
195 200 205
Gly Gln Gly Val Leu Thr Ala Asn Ala Gln Gly Gln Leu Glu Ser Gln
210 215 220
Val Glu Met Gln Phe Phe Ser Thr Ser Glu Asn Ala Arg Asn Glu Ala
225 230 235 240
Asn Asn Ile Gln Pro Lys Leu Val Leu Tyr Ser Glu Asp Val His Met
245 250 255
Glu Thr Pro Asp Thr His Leu Ser Tyr Lys Pro Ala Lys Ser Asp Asp
260 265 270
Asn Ser Lys Ile Met Leu Gly Gln Gln Ser Met Pro Asn Arg Pro Asn
275 280 285
Tyr Ile Gly Phe Arg Asp Asn Phe Ile Gly Leu Met Tyr Tyr Asn Ser
290 295 300
Thr Gly Asn Met Gly Val Leu Ala Gly Gln Ala Ser Gln Leu Asn Ala
305 310 315 320
Val Val Asp Leu Gln Asp Arg Asn Thr Glu Leu Ser Tyr Gln Leu Leu
325 330 335
Leu Asp Ser Met Gly Asp Arg Thr Arg Tyr Phe Ser Met Trp Asn Gln
340 345 350
Ala Val Asp Ser Tyr Asp Pro Asp Val Arg Ile Ile Glu Asn His Gly
355 360 365
Thr Glu Asp Glu Leu Pro Asn Tyr Cys Phe Pro Leu Gly Gly Ile Gly
370 375 380
Val Thr Asp Thr Tyr Gln Ala Val Lys Thr Asn Asn Gly Asn Asn Gly
385 390 395 400
Gly Gln Val Thr Trp Thr Lys Asp Glu Thr Phe Ala Asp Arg Asn Glu
405 410 415
Ile Gly Val Gly Asn Asn Phe Ala Met Glu Ile Asn Leu Ser Ala Asn
420 425 430
Leu Trp Arg Asn Phe Leu Tyr Ser Asn Val Ala Leu Tyr Leu Pro Asp
435 440 445
Lys Leu Lys Tyr Asn Pro Ser Asn Val Asp Ile Ser Asp Asn Pro Asn
450 455 460
Thr Tyr Asp Tyr Met Asn Lys Arg Val Val Ala Pro Gly Leu Val Asp
465 470 475 480
Cys Tyr Ile Asn Leu Gly Ala Arg Trp Ser Leu Asp Tyr Met Asp Asn
485 490 495
Val Asn Pro Phe Asn His His Arg Asn Ala Gly Leu Arg Tyr Arg Ser
500 505 510
Met Leu Leu Gly Asn Gly Arg Tyr Val Pro Phe His Ile Gln Val Pro
515 520 525
Gln Lys Phe Phe Ala Ile Lys Asn Leu Leu Leu Leu Pro Gly Ser Tyr
530 535 540
Thr Tyr Glu Trp Asn Phe Arg Lys Asp Val Asn Met Val Leu Gln Ser
545 550 555 560
Ser Leu Gly Asn Asp Leu Arg Val Asp Gly Ala Ser Ile Lys Phe Glu
565 570 575
Ser Ile Cys
<210> 6
<211> 37204
<212> DNA
<213> 人工序列
<220>
<223> BZ1基因组
<400> 6
catcatcaat aatatacctt attttggatt gtggccaata tgataatgag gtgggcgggg 60
cgaggcgggg cgggtgacgt aggacgtgcg agtagggttg ggaggtgtgg cggaagtgtg 120
gcatttgtaa gtgggaggag cttacatgca agcttccgtc gcggaaaatg tgacgttttt 180
tatgagcgcc gcctacctcc ggaagtgcca attttcgcgc gctttttacc ggatatcgta 240
gtaattttgg gcgggaccat gtaagatttg gccattttcg cgcgaaaagt gaaacgggga 300
agtgaaaact gaataatagg gcgttagtca tagcgcgtaa tatttaccga gggccgaggg 360
actttgaccg attacgtgga ggactcgccc aggtgttttt tacgtgaatt tccgcgttcc 420
gggtcaaagt ctccgttttt attgtcaccg tcatttgacg cggagggtat ttaaacccgc 480
tgcgctccta aagaggccac tcttgagtgc cagcgagaag agttttctcc tccgctctgt 540
ttcggcgatc gaaaaatgag acatttagcc tgcactccgg gtcttctgtc cggccgggcg 600
gcgtccgagc ttttggacgc tttgctcaat gaggtcctga gcgatgattt tccgtctact 660
acccacttta gcccacctac tcttcacgaa ctgtacgatc tggatgtact ggtggatgtg 720
aacgatccta acgaggaggc ggtttctacg ttttttcccg agtctgcgct tttggccgcc 780
caggagggat ttgacctaca cactccgccg ctgcctattt tagagtctcc gctgccggag 840
cccagtggta taccttatat gcctgaactg cttcccgaag tggtagacct gacctgccac 900
gagccgggct ttccgcccag cgacgatgag ggtgagcctt ttgttttaga ttatgctgag 960
atacccgggc tcggttgcag gtcttgtgca tatcatcaaa gggttaccgg agaccccgag 1020
gttaagtgtt cgctgtgcta tatgaggctg acctcttcct ttatctacag taagtttttt 1080
gtgtaggtgg gctttttggg taggtgggtt ttgtggcagg acaggtgtaa atgttgcttg 1140
tgtttttttg tacctgcagg tccggtgtcc gagccagacc cggagcccga ccgcgatccc 1200
gagccggatc ccgagcctcc tcgcagggca aggaaattac cttctattct gtgcaagcct 1260
aagacacctg taaggaccag cgaggcagac agcactgact ctggcacttc tacctctcct 1320
cctgaaattc acccagtggt tcctttgggt atacataaac ctgttgctat tagagtttgc 1380
gggcgacgcc ctgcagtaga gtgcattgag gacttgctta acgatcccga gggacctttg 1440
gacttgagca ttaaacgccc taggcaataa accccaccta agtaataaac cccacctaag 1500
taataaactt taccgccctt ggttattaag atgacgccca atgtttgctt ttgaatgact 1560
tcatgtgtat aataaaagtg agtgtggtca taggtctctt gtttgtctgg gcggggctta 1620
agggtatata agtttctcgg ggctaaactt ggttacactt gaccccaatg gaggcgtggg 1680
ggtgcttgga ggagtttgcg gacgtgcgcc gtttgctgga cgagagctct agcaatacct 1740
atagtatttg gaggtatctg tggggctcta ctcaggccaa gttggtttgc agaattaagc 1800
aggattacaa gtgcgatttt gaagagcttt ttagttcctg tggtgagctt ttgcaatcct 1860
tgaatctggg ccaccaggct atcttccagg aaaaggttct ctcgactttg gatttttcca 1920
ctcccgggcg caccgccgct tgtgtggctt ttgtgtcttt tgtgcaagat aaatggagcg 1980
gggagaccca cctgagtcac ggctacgtgc tggatttcat ggcgatggct ctttggaggg 2040
cttacaacaa atggaagatt cagaaggaac tgtacggttc cgccctacgt cgtccacttc 2100
tgcagcggca ggggctgatg tttcccgacc atcgccagca tcagaatctg gaagacgagt 2160
cggaggagcg agcggagaag atcagcttga gagccggcct ggaccctcct caggaggaat 2220
gaatctcccg caggtggttg agctgtttcc cgaactgaga cgggtcctga ctatcaggga 2280
ggatggtcag tttgtgaaga agctgaagag ggatcggggt gagggagatg atgaggcggc 2340
tagcaattta gcttttagtc tgataacccg ccaccgaccg gaatgtatta cctatcagca 2400
gattaaggag agttgtgcca acgagctgga tcttttgggt cagaagtata gcatagaaca 2460
gcttaccact tactggcttc agcccgggga tgattgggaa gaggcgatca gggtgtatgc 2520
aaaggtggcc ctgcggcccg attgcaagta taagattact aagttggtta atattagaaa 2580
ctgctgctat atttctggaa acggggccga agtggagata gatactgagg acagggtggc 2640
tattaggtgt tgcatgataa acatgtggcc cgggatactg gggatggatg gggtgatatt 2700
tatgaatgtg aggttcacgg gccccaactt taatggtacg gtgtttatgg gcaacaccaa 2760
cttgctcctg catggtgcga gtttctatgg gtttaacaac acctgtatag aggcctggac 2820
cgatgtaaag gttcgaggtt gttcctttta tagctgttgg aaggcggtgg tgtgtcgccc 2880
taaaagcagg ggttctgtga agaaatgctt gtttgaaagg tgtaccctag gtatcctttc 2940
tgagggcaac tccagggtgc gccataatgt ggcttcgaac tgcggttgct ttatgcaagt 3000
gaagggggtg agcgttatca agcataactc ggtctgtgga aactgcgagg atcgcgcctc 3060
tcagatgctg acctgctttg atggcaactg tcacctgttg aagaccattc atataagcag 3120
tcaccccaga aaggcctggc ccgtgtttga gcataacatt ctgacccgct gttccttgca 3180
tctgggggtc aggaggggta tgttcctgcc ttaccagtgt aacttcagtc acactaaaat 3240
cctgttggaa cccgagtgca tgaccaaggt cagcctgaat ggtgtgtttg atgtgagtct 3300
gaagatttgg aaggtgctga ggtatgatga gaccaggacc aggtgccgac cctgcgagtg 3360
cggcggcaag cacatgagaa atcagcctgt gatgttggat gtgaccgagg agcttaggcc 3420
tgaccatctg gtgctggcct gcaccagggc cgagtttggg tctagcgatg aggataccga 3480
ttgaggtggg taaggtgggc gtggctagca gggtgggcgt gtataaattg ggggtctaag 3540
gggtctctct gtttgtcttg caacagccgc cgccatgagc gacaccggca acagctttga 3600
tggaagcatc tttagcccct atctgacagt gcgcatgcct cactgggccg gagtgcgtca 3660
gaatgtgatg ggttccaacg tggatggacg tcccgttctg ccttcaaatt cgtctacgat 3720
ggcctacgcg accgtgggag gaactccgct ggacgccgcg acctccgccg ccgcctccgc 3780
cgccgccgcg accgcgcgca gcatggctac ggacctttac agctctttgg tggcgagcag 3840
cgcggcctct cgcgcgtctg ctcgggatga aaaactgact gctctgctgc ttaaactgga 3900
agacttgacc cgggagctgg gtcaactgac ccagcaggtc tccagcttgc gtgagagcag 3960
ccttgcctcc ccctaatggc ccataatata aataaaagcc agtctgtttg gattaagcaa 4020
gtgtatgttc tttatttaac tctccgcgcg cggtaagccc gggaccagcg gtctcggtcg 4080
tttagggtgc ggtggattct ttccaacacg tggtacaggt ggctctggat gtttagatac 4140
atgggcatga gtccatccct ggggtggagg tagcaccact gcagagcttc gtgctcgggg 4200
gtggtgttgt atatgatcca gtcgtagcag gagcgctggg cgtggtgctg aaaaatgtcc 4260
ttaagcaaga ggcttatagc tagggggagg cccttggtgt aagtgtttac aaatctgctt 4320
agctgggagg ggtgcatccg gggggatatg atgtgcatct tggactggat ttttaggttg 4380
gctatgttcc cacccagatc ccttctggga ttcatgttgt gcaggaccac cagcacggta 4440
tatccagtgc acttgggaaa tttatcgtgg agcttagacg ggaatgcatg gaagaacttg 4500
gagacgccct tgtggcctcc cagattttcc atacattcgt ccatgatgat ggcaatgggc 4560
ccgtgggaag ctgcctgagc aaaaacgttt ctggcatcgc tcacatcgta gttatgttcc 4620
agggtgaggt catcatagga catctttaca aatcgggggc ggagggtccc ggactggggg 4680
atgatggtac cctcgggccc cggggcgtag ttcccctcac agatctgcat ctcccaggct 4740
ttcatttcag agggagggat catatccacc tgcggggcga tgaaaaagac agtttctggc 4800
gcaggggaga ttaactggga tgagagcagg tttctgagca gctgtgactt tccacagccg 4860
gtgggcccat atatcacgcc tatcaccggc tgcagctggt agttaagaga gctgcagctg 4920
ccgtcctccc ggagcagggg ggccacctcg ttgagcatat ccctgacgtg gatgttctcc 4980
ctgaccagtt ccgccagaag gcgctcgccg cccagcgaaa gcagctcttg caaggaagca 5040
aaatttttca gcggtttcag gccatcggcc gtgggcatgt ttttcagcgt ctgggtcagc 5100
agctccagcc tgtcccagag ctcggtgatg tgctctacgg catctcgatc cagcagatct 5160
cctcgtttcg cgggttgggg cggctttcgc tgtagggcac cagccgatgg gcgtccagcg 5220
gggccagagt catgtccttc catgggcgca gagtcctcgt cagggtggtc tgggtcacgg 5280
tgaaggggtg cgctccgggt tgggcactgg ccagggtgcg cttgaggctg gttctgctgg 5340
tgctgaatcg ctgccgctct tcgccctgcg cgtcggccag gtagcatttg accatggtct 5400
cgtagtcgag accctcggcg gcgtgcccct tggcgcggag ctttcccttg gaggtggcgc 5460
cgcacgaggg gcactgcagg ctcttcaggg cgtagagctt gggagcgaga aacacggact 5520
ctggggagta ggcgtccgcg ccgcaggccg cgcagaccgt ctcgcattcc accagccaag 5580
tgagttccgg gcggtcaggg tcaaaaacca ggttgccccc atgctttttg atgcgtttct 5640
tacctcggct ctccatgagg cggtgtccct tctcggtgac gaagaggctg tccgtgtccc 5700
cgtagaccga cttcaggggc ctgtcttcca gcggagtgcc tctgtcctcc tcgtagagaa 5760
actctgacca ctctgagacg aaggcccgtg tccaggccag gacgaaggag gccacgtggg 5820
aggggtagcg gtcgttgtcc actagcgggt ccaccttctc cagggtgtgc agacacatgt 5880
ccccctcctc cgcgtccaga aaagtgattg gcttataggt gtaggacacg tgaccggggg 5940
ttcccgacgg gggggtataa aagggggtgg gcgccctttc atcttcactc tcttccgcat 6000
cgctgtctgc gagagccagc tgctggggta agtattccct ttcaaaggcg ggcatgacct 6060
cagcgctcag gttgtcagtt tctaaaaatg aggaggattt gatgttcacc tgtccggaag 6120
tgataccttt gagggtacct gggtccatct ggtcagaaaa cactattttt ttgttgtcaa 6180
gcttggtggc gaacgacccg tagagggcgt tggagagcag cttggcaatg gagcgcaggg 6240
tctggttttt gtcgcggtcg gctcgctcct tggccgcgat gttgagttgc acgtattcgc 6300
gggccacgca cttccactcg gggaagacgg tggtgcgctc gtctgggatc aggcgcaccc 6360
tccagccgcg gttgtgcagg gtgaccatgt cgacgctggt ggcgacctca ccgcgcagac 6420
gctcgttggt ccagcagagg cggccgcctt tgcgcgagca gaaggggggt agggggtcca 6480
gctggtcctc gtttgggggg tccgcgtcga tggtaaagac cccggggagc aggcgcgggt 6540
caaagtagtc gatcttgcaa gcttgcatgt ccagagcccg ctgccattcg cgggcggcga 6600
gcgcgcgctc gtaggggttg aggggcgggc cccagggcat ggggtgggtg agcgcggagg 6660
cgtacatgcc gcagatgtca tacacgtaca ggggttccct gaggataccg aggtaggtgg 6720
ggtagcagcg ccccccgcgg atgctggcgc gcacgtagtc atagagctcg tgggaggggg 6780
ccagcatgtt gagcccaagg ttggtgcgct gggggcgctc ggcgcggaag acgatctgtc 6840
tgaagatggc atgggagttg gaggagatgg tgggtcgctg gaagacgttg aagcttgctt 6900
cttgcaagcc cacggagtcc ctgacgaagg aggcgtagga ctcgcgcagc ttgtgcacca 6960
gctcggcggt gacctggacg tcgagcgcgc agtagtcgag ggtctcgcgg atgatgtcat 7020
acttatcctc ccccttcttt ttccacagct cgcggttgag gacgaactct tcgcggtctt 7080
tccagtactc ttggagggga aacccgtccg tgtccgaacg gtaagagcct agcatgtaga 7140
actggttgac ggcctggtag gggcagcagc ccttctccac gggcagcgcg taggcctgcg 7200
ccgccttgcg gagggaggtg tgggtaaggg cgaaagtgtc cctgaccatg actttgaggt 7260
attgatgtct gaagtctgtg tcatcgcagc cgccctgttc ccacagggtg tagtccgtgc 7320
gctttttgga gcgcgggttg ggcagggaga aggtgaggtc attgaagagg atcttccccg 7380
ctcgaggcat gaagtttctg gtgatgcgaa agggccctgg gaccgaggag cggttgttga 7440
tgacctgggc ggccaggacg atctcgtcaa agccgtttat gttgtggccc acgatgtaga 7500
gctccaggaa gcggggctgg cccttgatgg aggggagctt tttaagttcc tcgtaggtaa 7560
gctcctcggg cgattccagg ccgtgctcct ccagggccca gtcttgcaag tgagggttgg 7620
ccgccaggaa ggatcgccag aggtcgcggg ccatgagggt ctgcaggcgg tcgcggaagg 7680
ttctgaactg ccgccctacg gccatctttt cgggggtgat gcagtagaag gtgagggggt 7740
ctttctccca ggggtcccat ctgagctctt gggcgaggtc gcgcgcggcg gcgaccagag 7800
cctcgtcgcc ccccagtttc atgaccagca tgaagggcac gagctgcttg ccaaaggctc 7860
ccatccaagt gtaggtctct acatcgtagg tgacaaagag gcgctccgtg cgaggatgag 7920
agccgatcgg gaagaactgg atctcccgcc accagttgga ggattggctg ttgatgtggt 7980
gaaagtagaa gtcccgtctg cgggccgagc actcgtgctg gcttttgtaa aagcgaccgc 8040
agtactggca gcgctgcacg ggttgtatat cttgcacgag gtgaacctgg cgacctctga 8100
cgaggaagcg cagcgggaat ctaagtcccc cgcctggggt cccgtgtggc tggtggtctt 8160
ctactttggt tgtctggccg ccagcatctg tctcctggag ggcgatggtg gagcagacca 8220
ccacgccgcg agagccgcag gtccagatct cggcgctcgg cgggcggagt ttgatgacga 8280
catcgcgcac attggagctg tccatggtct ccagctcccg cggcggcagg tcagccggga 8340
gttcctggag gttcacctcg cagagacggg tcaaggcgcg gacagtgttg agatggtatc 8400
tgatttcaag gggcgtgttg gaggcggagt cgatggcttg cagaaggccg cagccccggg 8460
gggccacgat ggttccccgc ggggcgcgag gggaggcgga agctgggggt gtgttcagaa 8520
gcggtgacgc gggcgggccc ccggaggtag ggggggttcc ggccccacag gcatgggcgg 8580
caggggcacg tcttcgccgc gcgcgggcag gggctggtgc tggctccgga gagcgcttgc 8640
gtgcgcgacg acgcgacggt tggtgtcctg tatctggcgc ctctgagtga agaccacggg 8700
tcccgtgacc ttgaacctga aagagagttc gacagaatca atctcggcat cgttgacagc 8760
ggcctggcgc aggatctcct gcacgtcgcc cgagttgtcc tggtaggcga tctctgccat 8820
gaactgctcg atctcttcct cctggagatc tcctcgtccg gcgcgctcca cggtggccgc 8880
caggtcgttg gagatgcgac ccatgagctg cgagaaggcg ttgaggccgc cctcgttcca 8940
gacccggctg tagaccacgc ccccctcggc gtcgcgggcg cgcatgacca cctgggccag 9000
gttgagctcc acgtgtcgcg tgaagacggc gtagttgcgc aggcgctgga aaaggtagtt 9060
cagggtggtg gcggtgtgtt cggcgacgaa gaagtacatg acccagcgcc gcaacgtgga 9120
ttcattgatg tcccccaagg cctccaggcg ctccatggcc tcgtagaagt ccacggcgaa 9180
gttgaaaaac tgggagttgc gagcggacac ggtcaactcc tcctccagaa gacggatgag 9240
ctcggcgaca gtgtcgcgca cctcgcgctc gaaggccacg gggggcgctt cttcctcttc 9300
cacctcttct tccatgattg cttcttcttc ctcagccggg acgggagggg gcggcggcgg 9360
gggaggggcg cggcggcggc ggcggcgcac cggcaggcgg tcgatgaagc gctcgatcat 9420
ctccccccgc atgcggcgca tggtctcggt gacggcgcgg ccgttctccc gggggcgcag 9480
ctcaaagacg ccgcctctca tctcgccgcg gggcgggcgg ccgtgaggta gcgagacggc 9540
gctgactatg catcttaaca attgctgtgt aggtacgccg ccaagggacc tgattgagtc 9600
cagatccacc ggatccgaaa acctttggag gaaagcgtct atccagtcgc agtcgcaagg 9660
taggctgagc accgtggcgg gcgggggcgg gtcgggagag ttcctggcgg agatgctgct 9720
gatgatgtaa ttaaagtagg cggtcttgag aaggcggatg gtggacagga gcaccatgtc 9780
tttgggtccg gcctgttgga tgcggaggcg gtcggccatg ccccaggcct cgttctgaca 9840
ccggcgcagg tctttgtagt agtcttgcat gagtctttcc accggcacct cttctccttc 9900
ctcttctcca tctcgccggt ggtttctcgc gccgcccatg cgcgtgaccc caaagcccct 9960
gagcggctgc agcagggcca ggtcggcgac cacgcgctcg gccaagatgg cctgctgtac 10020
ctgagtgagg gtcctctcga agtcatccat gtccacgaag cggtggtagg cgcccgtgtt 10080
gatggtgtag gtgcagttgg ccatgacgga ccagttgacg gtctggtgtc ccggctgcga 10140
gagctccgtg taccgcaggc gcgagaaggc gcgggaatcg aacacgtagt cgttgcaagt 10200
ccgcaccaga tactggtagc ccaccaggaa gtgcggcgga ggttggcgat agaggggcca 10260
gcgctgggtg gcgggggcgc cgggcgccag gtcttccagc atgaggcggt ggtatccgta 10320
gatgtacctg gacatccagg tgatgccggc ggcggtggtg gtggcgcgcg cgtagtcgcg 10380
gacccggttc cagatgtttc gcaggggcga gaagtgttcc atggtcggca cgctctggcc 10440
ggtgaggcgc gcgcagtcgt tgacgctcta tacacacaca aaaacgaaag cgtttacagg 10500
gctttcgttc tgtagcctgg aggaaagtaa atgggttggg ttgcggtgtg ccccggttcg 10560
agaccaagct gagctcggcc ggctgaagcc gcagctaacg tggtattggc agtcccgtct 10620
cgacccaggc cctgtatcct ccaggatacg gtcgagagcc cttttgcttt cttggccaag 10680
cgcccgtggc gcgatctggg atagatggtc gcgatgagag gacaaaagcg gctcgcttcc 10740
gtagtctgga gaaacaatcg ccagggttgc gttgcggcgt accccggttc gagcccctat 10800
ggcggcttgg atcggccgga accgcggcta acgtgggctg tggcagcccc gtcctcagga 10860
ccccgccagc cgacttctcc agttacggga gcgagcccct tttgtttttt attttttaga 10920
tgcatcccgt gctgcggcag atgcgcccct cgccccggcc cgatcagcag cagcaacagc 10980
aggcatgcag acccccctct cctctacccg ccccggtcac cacggccgcg gcggccgtgt 11040
ccggcgcggg gggcgcgctg gagtcagatg agccaccgcg gcggcgacct aggcagtatc 11100
tggacttgga agagggcgag ggactggcgc ggctgggggc gagctctcca gagcgccacc 11160
cgcgggtgca gttgaaaagg gacgcgcgcg aggcgtacct gccgcggcag aacctgtttc 11220
gcgaccgcgg gggcgaggag cccgaggaga tgcgagactg caggttccaa gcggggcgcg 11280
agctgcgccg cgggttggac agacagcgcc tgctgcgcga ggaggacttt gagcccgaca 11340
cgcagacggg catcagcccc gcgcgcgcgc acgtggccgc ggccgacctg gtgaccgcct 11400
acgagcagac ggtgaaccag gagcgcaact tccaaaaaag cttcaacaac cacgtgcgca 11460
cgctggtggc gcgcgaggag gtgaccctgg gtctcatgca tctgtgggac ctggtggagg 11520
cgatcgtgca gaaccccagc agcaagcccc tgaccgcgca gctgttcctg gtggtgcagc 11580
acagcaggga caacgaggcc ttcagggagg cgctgctgaa catcaccgag ccggaggggc 11640
gctggctcct ggacctgata aacatcctgc agagcatagt ggtgcaggag cgcagcctga 11700
gcctggccga gaaggtggcg gccattaact attctatgct gagcttgggc aagttctacg 11760
cccgcaagat ctacaagacc ccctacgtgc ccatagacaa ggaggtgaag atagacagct 11820
tctacatgcg catggcgcta aaggtgctga ccctgagcga cgacctggga gtgtaccgca 11880
acgagcgcat ccacaaggcc gtgagcgcca gccggcggcg cgagctgagc gaccgcgagc 11940
tgatgcacag tctgcagcgc gcgctcaccg gcgcgggcga gggcgacagg gaggtcgagt 12000
cctacttcga catgggggct gacctgcact ggcagccgag ccgccgcgcc ctggaggcgg 12060
cgggggcgta tggcggcccc ctggcggccg atgacgagga agaggaggac tatgagctag 12120
aggagggcga gtacctggag gactgacctg gctggtggtg ttttggtata gatgcaagat 12180
ccgaacgtgg cggacccggc ggtccgggcg gcgctgcaga gccagccgtc cggcattaac 12240
tcctctgacg actgggccgc ggccatgggt cgcatcatgg ccctgaccgc gcgcaacccc 12300
gaggccttca ggcagcagcc tcaggctaac cggctggcgg ccatcttgga agcggtagtg 12360
cccgcgcgct ccaaccccac ccacgagaag gtgctggcca tagtcaacgc gctggcggag 12420
agcagggcca tccgggcgga cgaggccgga ctggtgtacg atgcgctgct gcagcgggtg 12480
gcgcggtaca acagcggcaa cgtgcagacc aacctggacc gcctggtgac ggacgtgcgc 12540
gaggccgtgg cgcagcgcga gcgcttgcat caggacggta acctgggctc gctggtggcg 12600
ctaaacgcct tcctcagcac ccagccggcc aacgtaccgc gggggcagga ggactacacc 12660
aacttcttga gcgcgctgcg gctgatggtg accgaggtcc ctcagagcga ggtgtaccag 12720
tcggggcccg actacttctt ccagaccagc agacagggct tgcaaaccgt gaacctgagc 12780
caggctttca agaacctgcg ggggctgtgg ggagtgaaag cgcccaccgg cgaccgagct 12840
acggtgtcca gcctgctaac ccccaactcg cgcctgctgc tgctgctgat cgcgcccttc 12900
acggacagcg ggagcgtctc gcgggagacc tatctgggcc acctgctgac gctgtaccgc 12960
gaggccatcg ggcaggcgca ggtggacgag cacaccttcc aagagatcac cagcgtgagc 13020
cacgcgctgg ggcaggagga cacgggcagc ctgcaggcga ccctgaacta cctgctgacc 13080
aacaggcggc agaagattcc cacgctgcac agcctgaccc aggaggagga gcgcatcttg 13140
cgctacgtgc agcagagcgt gagcctgaac ctgatgcgcg acggcgtgac gcccagcgtg 13200
gcgctggaca tgaccgcgcg caacatggaa ccgggcatgt acgcttccca gcggccgttc 13260
atcaaccgcc tgatggacta cttgcatcgg gcggcggccg tgaaccccga gtacttcacc 13320
aatgccattc tgaatcccca ctggatgccc cctccgggtt tctacaacgg agacttcgag 13380
gtgcctgagg tcaatgatgg gttcctctgg gatgacatgg atgacagtgt gttctccccc 13440
aacccgctgc gcgccgcgtc tctgcgattg aaggagggct ctgacaggga aggaccgagg 13500
agtctggcct cctccctggc tctgggggcg gtgggcgcca cgggcgcggc ggcgcggggc 13560
agcagcccct tccccagcct ggcagactct ctgaatagcg ggcgggtgag caggccccgc 13620
ttgctaggcg aggaggagta tctgaacaac tccctgctgc agcccgtgag ggacaaaaac 13680
gctcagcgac agcagtttcc caacaacggg atagagagcc tggtggacaa gatgtccaga 13740
tggaagacat atgcgcagga gtacaaggag tgggaggacc gccagccgcg gcccctgccg 13800
ccccctagac agcgctggca gcggcgcgcg tccaaccgcc gctggagaca gggacccgag 13860
gacgatgatg actctgcaga tgacagcagc gtgttggacc tgggcgggag cgggaacccc 13920
ttttcgcacc tgcgcccacg cctgggcaag atgttttaaa agagaaaaat aaaactcacc 13980
aaggccatgg cgacgagcgt tggttttttg ttcccttcct tagtatgcgg cgcgcggcga 14040
tgttcgagga ggggcctccc ccctcttacg agagcgcgat gggaatttct cctgcggcgc 14100
ccctgcagcc tccctacgtg cctcctcggt acctgcaacc tacagggggg agaaatagca 14160
tctgttactc tgagctgcag cccctgtacg ataccaccag actgtacctg gtggacaaca 14220
agtccgcgga cgtggcctcc ctgaactacc agaacgacca cagcgatttt ttgaccacgg 14280
tgatccaaaa caacgacttc accccaaccg aggccagtac ccagaccata aacctggaca 14340
acaggtcgaa ctggggcggc gacctgaaga ccatcctgca caccaacatg cccaacgtga 14400
acgagttcat gtttaccaac tcttttaagg cgcgggttat ggtggcgcgc gagcaggggg 14460
aggcgaagta cgagtgggtg gacttcacgc tgcccgaggg caactactca gagaccatga 14520
ctattgacct gatgaacaat gcgatcgtgg aacactacct gaaagtgggc aggcagaacg 14580
gggtgaagga gagcgatatc ggggtcaagt ttgacaccag aaactttcgt ctgggctggg 14640
accccgtgac cgggctggtc atgccggggg tctacaccaa cgaggccttt catcccgata 14700
tagtgctcct gcccggctgt ggggtggact ttacccagag ccggctgagc aacctgctgg 14760
gcgttcgcaa gcggcaacct ttccaggagg gtttcaagat cacctatgag gatctggagg 14820
ggggcaacat tcccgcgctc cttgatctgg acgcctacga ggagagcttg aaacccgagg 14880
agagcgctgg cgacagcggc gagagtggcg aggagcaagc cggcggcggt ggcagcgcgt 14940
cggtagaaaa cgaaagtact cccgcagtgg cggcagacgc tgcggaggtc gagccagagg 15000
ccatgcagca ggacgcagag gagggcgcac aggagggcgc gcaggaggac atgaacgatg 15060
gggagatcag gggagacact ttcgccaccc ggggcgaaga aaaagaggca gaggcggtgg 15120
cggcgacggt ggaagccgaa accgaggcag aggcagagcc caagaccgaa gttatggaag 15180
acatgaatga tggagaacgt aggggtgaca cgtttgccac ccggggcgaa gagaaggcgg 15240
cggaggcaga agccgcggct gaggaggcgg ctgcggctgc ggccgaggct gaggctgcgg 15300
ctgaggctaa ggtcgaagcc gatgttgcgg ttgaggctca ggctgaggag gaggaggcgg 15360
cgactgaagc agttaaggaa aaggcccagg cagagcagga agagaaaaaa cctgtcattc 15420
aacctctaaa agaagatagc aaaaagcgca gttacaacgt catcgagggc agcaccttta 15480
cccagtaccg cagctggtac ctggcgtaca actacggcga cccggtcaag ggggtgcgct 15540
cgtggaccct gctctgcacg ccggacgtca cctgcggctc cgagcagatg tattggtcgc 15600
tgccaaacat gatgcaagac ccggtgacct tccgctccac gcggcaggtt agcaacttcc 15660
cggtggtggg cgccgaactg ctgcccgtgc actccaagag tttttacaac gagcaggccg 15720
tctactccca gctgatccgc caggccacct ctctgaccca cgtgttcaat cgctttcccg 15780
agaaccagat tttggcgcgc ccgccggccc ccaccatcac caccgtcagt gaaaacgttc 15840
ctgccctcac agatcacggg acgctaccgc tgcgcaacag catctcagga gtccagcgag 15900
tgaccattac tgacgccaga cgccggacct gcccctacgt ttacaaggcc ttgggcatag 15960
tctcgccgcg cgtcctctcc agtcgcactt tttaaaatac atctaccctc acgcttcaaa 16020
atcatgtccg tactcatctc acccagcaac aacaccggct gggggctgcg cgcgcccagc 16080
aagatgtttg gaggggcgag gaaacgctcc gagcagcacc cagtgcgcgt gcgcggccac 16140
taccgcgcgc cctggggagc gcacaagcgc gggcgcgcag ggcgcaccac tgtggacgac 16200
gccattgact ccgtagtgga gcaggcgcgc cactacacac ccggcgcgcc gtccgccccc 16260
gccgtgtcca ccgtggacga ggcgatcgag agcgtggtac agggcgcgcg gcactatgcc 16320
aaccttaaaa atcgacgccg tcgcgtggct cgccgccatc gccggagacc ccgggccacc 16380
gccgccgcgc gccttgctaa ggctctgctc aggcgcgcca ggcgaactgg ccgccgggcc 16440
gccatgaggg ccgcacggcg ggctgccgcc agcgcggccg ccgcggcccc acgggcacga 16500
aggcgcgcgg ccgctgccgc cgccgccgcc atttccagct tggcctcgac gcggcgcggt 16560
aacatatact gggtgcgcga ctcggtaacc ggcacgcggg tacccgtgcg ctttcgcccc 16620
ccgcggaatt agcacaagac aacatacaca ctgagtctcc tgctgttgtg tatcccagcg 16680
gcgaccgtca gcagcggcga catgtccaag cgcaaaatta aagaagagat gctccaggtc 16740
atcgcgccgg agatctatgg gcccccgaag aaggaggagg atgattacaa gccccgcaag 16800
ctaaagcggg tcaaaaagaa aaagaaagat gatgacgttg acgaggcggt ggagtttgtc 16860
cgccgcatgg cgcccaggcg ccccgtgcag tggaagggtc ggcgcgtgca gcgagtcctg 16920
cgccccggca ccgcggtggt ctttacgccc ggcgagcgtt ccacgcgcac tttcaagcgg 16980
gtgtacgatg aggtgtacgg cgacgaggat ctgttggagc aggccaacca tcgctttggg 17040
gagtttgcat atgggaaacg gccccgcgag agcctaaaag aggacctgct ggcgctaccg 17100
ctggacgagg gcaatcccac cccgagtctg aagccggtaa ccctgcaaca ggtgctgcct 17160
ttgagcgcgc ccagcgagca gaagcgaggg ttgaagcgcg agggcgggga cctggcaccc 17220
accgtgcagt tgatggtgcc caagcggcag aagctggagg acgtgctgga gaaaatgaaa 17280
gtagagcccg ggatccagcc cgaaatcaag gtccgcccca tcaagcaggt ggcgcccggc 17340
gtgggagtcc agaccgtgga cgttaggatt cccacggagg agatggaaac ccaaaccgcc 17400
actccctctt cggcggctag cgccaccacc ggctccgctt cggtagaggt gcagacggac 17460
ccctggctag ccgccgccgc cccggccgcc ccccgttcgc gcgggcgcaa gagaaattat 17520
ccagcggcca gcgcgctcat gccccagtac gcactgcatc catccatcgc gcccaccccc 17580
ggctaccgcg ggtactcgta ccgcccgcgc agatcagccg gcacccgcgg ccgccgccgc 17640
cgtgcgacca caaccagccg ccgccgtcgc cgccgccgcc agccagtgct gacccccgtg 17700
tctgtaagga aggtggctcg ctcggggagc acgctggtgg tgcccagagc gcgctaccac 17760
cccagcattg tttaaagccg gtctctgtat ggttcttgca gatatggccc tcacttgtcg 17820
cctccgcttc ccggtgccgg gataccgagg aagaactcac cgccgcagag gcatggcggg 17880
cagtggtctc cgcggcggcc gtcgccatcg ccggcgcgca aagagcaggc gcatgcgcgg 17940
cggtgtgctg cccttcctaa tcccgctaat cgccgcggcg atcggtgccg tgcccgggat 18000
cgcctccgtg gccctgcagg cgtcccagaa acattgactc ttgcaacctt gcaagcttgc 18060
attttttgga ggaaaaaata aaaagtctag actctcacgc tcgcttggtc ctgtgactat 18120
tttgtagaaa aaagatggaa gacatcaact ttgcgtcgct ggccccgcgt cacggctcgc 18180
gcccgttcat gggagactgg acagatatcg gcaccagcaa tatgagcggt ggcgccttca 18240
gctggggcag tctgtggagt ggccttaaaa attttggttc caccattaag aactatggca 18300
acaaagcgtg gaacagcagc acgggtcaga tgctgagaga caagttgaaa gagcagaact 18360
tccaggagaa ggtggcacag ggcctggcct ctggcatcag cggggtggtg gacatagcta 18420
accaggccgt gcagaaaaag ataaacagtc atctggaccc ccgccctcag gtggaggaaa 18480
cgcctccagc catggagacg gtgtctcccg agggcaaagg cgaaaagcgc ccgcggcccg 18540
acagggaaga gaccctggtg tcacacaccg aggagccgcc ctcttacgag gaggcagtca 18600
aggccggcct gcccaccact cgccccatag ctcccatggc caccggtgtg gtgggtcaca 18660
ggcaacacac ccccgcgaca ctagatctgc ccccgccgtc cgagccgact cgccagccaa 18720
aggcggtgac ggtgcccgct ccctccactt ccgccgccaa cagagtgcct ctgcgccgcg 18780
ctgcgagcgg cccccgggcc tcgcgagtca gcggcaactg gcagagcaca ctgaacagca 18840
tcgtgggcct gggagtgagg agtgtgaagc gccgccgttg ctactgaatg agcaagctag 18900
ctaacgtgtt gtatgtgtgt atgcgtccta tgtcgccgcc agaggagctg ttgagccgcc 18960
ggcgccgtct gcactccagc gaatttcaag atggcgaccc catcgatgat gcctcagtgg 19020
tcgtacatgc acatctcggg ccaggacgct tcggagtacc tgagccccgg gctggtgcag 19080
ttcgcccgcg ccacagacac ctacttcaac atgagtaaca agttcaggaa ccccactgtg 19140
gcgcccaccc acgatgtgac cacggaccgg tcgcagcgcc tgacgctgcg gttcatcccc 19200
gtggatcggg aggacaccgc ttactcttac aaggcgcggt tcacgctggc cgtgggcgac 19260
aaccgcgtgc tggacatggc ctccacttac tttgacatcc ggggggtgct ggacaggggc 19320
cccactttca agccctactc gggcactgcc tacaactccc tggcccccaa gggcgctccc 19380
aattcttgcg agtgggagga ggaaacacaa aatgaggtac aagccaatga agaacaacta 19440
gcagaagaag aggatgaaga aatggctcaa gaggatcagc agcctactaa aaaaacccat 19500
gtatatgctc aggcacctct ttctggcgaa cagattacca aagatggctt gcaaatagga 19560
gctgaagtta caggagaaac atcaaagccc atttttgcag acaagacatt ccaaccagaa 19620
cctcagatag gagagtctca atggaatgag gccgatgcta cagtagcagg aggtagggtt 19680
ttgaaaaaga ctacccctat gaaaccttgc tatggatcct atgccagacc taccaatgcc 19740
aatggagggc aggggatact tgaggcaaat gctaaagggg aactcgaatc taaagttgag 19800
atgcagtttt tctctaacac cacaactctt aatgtaagag acggtgaaaa tggccttaaa 19860
ccaaaagtag tgctgtatag cgaagatgtc aacctggaat cccctgacac tcatctgtct 19920
tacaagccca aaaaagatga tgttaatgcc aaaatcatgt tgggtcagca agccatgccc 19980
aacagaccca acctcattgg atttagagat aatttcattg ggctcatgta ttacaacagc 20040
actggaaaca tgggagtgct ggcgggtcag gcctctcagt tgaatgctgt ggtggacttg 20100
caggatagaa acacggaact gtcatatcag cttatgcttg attccattgg agatagaacc 20160
agatactttt ccatgtggaa ccaggcagtg gatagctatg acccagatgt tagaatcatt 20220
gaaaaccatg gggtggagga tgagctgccc aactactgtt ttcccttggg cggtatagga 20280
attacagata cataccaggc cataaaagca gccaatggtg gagatgctac tacgtggtct 20340
gctgataaca catttgcaga ccgcaacgaa ataggggtgg gaaacaactt cgccatggag 20400
atcaacatcc aggccaacct ctggagaaac ttcctctatg cgaacgtggg actctacctg 20460
ccagacaagc tcaagtacaa ccccaccaac gtggacatct ctgacaaccc caacacctat 20520
gactacatga acaagcgggt ggtggccccc ggcctggtgg actgctttgt caatgtggga 20580
gccaggtggt ccctggacta catggacaac gtcaacccct tcaaccacca ccgcaatgcg 20640
ggtctgcgct accgctccat gatcctgggc aacgggcgct atgtgccctt ccacatccag 20700
gtaccccaga agttctttgc catcaagaac ctcctgctcc tgcccggctc ctacacctac 20760
gagtggaact tcaggaagga tgtaaacatg gtcctacaga gctctctggg caatgacctt 20820
agggtagatg gggccagcat caagtttgac agcatcaccc tctatgctac atttttcccc 20880
atggcccaca acaccgcctc cacgcttgag gccatgctga gaaacgacac caacgaccag 20940
tccttcaatg actacctctc tggggccaac atgctctacc caatcccagc caaggccacc 21000
aacgtgccca tctccatccc ctctcgcaac tgggccgcct ttagaggctg ggcctttacc 21060
cgccttaaga ccaaggagac cccctccctg ggctcgggtt ttgatcccta ctttgtttac 21120
tcgggatcca tcccctacct ggatggcacc ttctacctca accacacttt caagaagata 21180
tccatcatgt atgactcctc cgtcagctgg ccgggcaacg accgcttgct cacccccaat 21240
gagttcgagg tcaagcgcgc cgtggacggc gagggctaca acgtggccca gtgcaacatg 21300
accaaggact ggttcctggt gcagatgctg gccaactaca acataggcta ccagggcttc 21360
tacatcccag agagctacaa ggacaggatg tattccttct tcagaaattt ccaacccatg 21420
agccgacagg tggtggacga gaccaattac aaggactatc aggccattgg catcacccac 21480
cagcacaaca actcgggttt cgtgggctac ctggcgccca ccatgcgcga gggacaggcc 21540
taccccgcca acttccccta ccccctgata ggcaagaccg cggtcgacag cgtcacccag 21600
aaaaagttcc tctgcgaccg caccctctgg cgcatcccct tctctagcaa ctttatgtcc 21660
atgggtgcgc tcacggacct gggccaaaac ctgctttatg ccaactctgc ccatgcgctg 21720
gacatgactt ttgaggtgga ccccatggac gagcccaccc ttctctatat tgtgtttgaa 21780
gtgttcgacg tggtcagagt gcaccagccg caccgcggtg tcatcgagac cgtgtacctg 21840
cgtacgccct tctcagccgg caacgccacc acctaaggag acagcgccgc cgcctgcatg 21900
actggttcca ccgagcaaga gctcagggcc atcgccagag acctgggatg cggaccctat 21960
tttttgggca cctatgacaa acgcttcccg ggttttatct cccgagacaa gctcgcctgc 22020
gccatcgtca acacggccgc gcgcgagacc gggggcgtgc actggctggc ctttggctgg 22080
gacccgcgct ctaaaacttg ctacctcttt gacccctttg gcttctccga tcagcgcctc 22140
aggcagattt atgagtttga gtacgagggg ctgttgcgcc gcagcgcgct tgcctcctcg 22200
cccgaccgct gcatcaccct tgagaagtcc accgagaccg tgcaggggcc ccactcggcc 22260
gcctgcggtc tcttctgttg catgtttttg cacgcctttg tgcactggcc tcagagtccc 22320
atggatcgca accccaccat gaacttgcta aagggagtgc ccaacgccat gctccagagc 22380
ccccaggtcc tgcccaccct gcgccgcaac caggaacagc tctaccgctt cctggagcgc 22440
cactccccct acttccgcag ccacagcgcg cgcatccggg gggccacctc tttttgccac 22500
ttgcaagaaa acatgcaaga cggaaaatga tgtacagcat gcttttaata aatgtaaaga 22560
ctgtgcactt tatttataca cgggctcttt ctggttattt attcaacacc gccgtcgcca 22620
tctagaaatc gaaagggttc tgccgcgcgt cgccgtgcgc cacgggcaga gacacgttgc 22680
gatactggaa gcggctcgcc cacttgaact cgggcaccac catgcggggc agtggttcct 22740
cggggaaatt ctcgctccac agggtgcggg tcagctgcag cgcgctcagg aggtcgggag 22800
ccgagatctt gaagtcgcag ttggggccgg aaccctgcgc gcgcgagttg cggtacacgg 22860
ggttgcagca ctggaacacc agcagggccg gattattcac gctggccagc aggctctcgt 22920
cgctaatcat gtcgctgtcc agatcctccg cgttgctcag ggcgaatggg gtcatcttgc 22980
agacctgcct gcccaggaaa ggcgggagcc caggcttgcc gttacagtcg cagcgcaggg 23040
gcattagcag gtgcccacgg cccgactgcg cctgcgggta caacgcgcgc atgaaggctt 23100
cgatctgcct aaaagccacc tgggtcttgg ctccctccga aaagaacatc ccacaggact 23160
tgctggagaa ctggttcgcg ggacagctgg catcgtgcag gcagcagcgc gcgtcagtgt 23220
tggcgatctg caccacgttg cgaccccacc ggtttttcac tatcttggcc ttggaagcct 23280
gctcctttag cgcgcgctgg ccgttctcgc tggtcacatc catctctatc acctgttcct 23340
tgttgatcat gtttgtcccg tgcagacact ttaggtcgcc ctccgtctgg gtgcagcggt 23400
gctcccacag cgcgcaaccg gtgggctccc aatttttgtg ggtcaccccc gcgtaggcct 23460
gcaggtaggc ctgcaggaag cgccccatca tggtcataaa ggtcttctgg ctcgtaaagg 23520
tcagctgcag gccgcgatgc tcttcgttca gccaggtctt gcagatggcg gccagcgcct 23580
cggtctgctc gggcagcatc ttaaaatttg tcttcaggtc gttatccacg tggtacttgt 23640
ccatcatggc acgcgccgcc tccatgccct tctcccaggc ggacaccatg ggcaggctta 23700
gggggtttat cacttccagc ggcgaggaca ccgtactttc gatttcttct tcctccccct 23760
cttcccggcg cgcgcccccg ctgttgcgcg ctcttaccgc ctgcaccaag gggtcgtctt 23820
caggcaagcg ccgcaccgag cgcttgccgc ccttgacctg cttgatcagt accggcgggt 23880
tgctgaagcc caccatggtc agcgccgcct gctcttcttc gtcttcgctg tctaccacta 23940
tttctgggga ggggcttctc cgctctgcgg caaaggcggc ggatcgcttc ttttttttct 24000
tgggagccgc cgcgatggag tccgccacgg cgaccgaggt cgagggcgtg gggctggggg 24060
tgcgcggcac cagggcctcg tcgccctcgg actcttcctc tgactccagg cggcggcgga 24120
gtcgcttctt tgggggcgcg cgcgttagcg gcggcggaga cggggacggg gacggggacg 24180
ggacgccctc cacagggggc ggtcttcgcg cagacccgcg gccgcgctcg ggggtcttct 24240
cgcgctggtc ttggtcccga ctggccattg tatcctcctc ctcctaggca gagagacata 24300
aggagtctat catgcaagtc gagaaggagg agagcttaac caccccctct gagaccgccg 24360
tcgccgtcgc ccccgctacc gccgacgcgc ccgccacacc gagcgacacc cccgcggacc 24420
cccccgccga cgcacccctg ttcgaggaag cggccgtgga gcaggacccg ggctttgtct 24480
cggcagagga ggatttgcaa gaggaggagg ataaggagga gaagccctca gtgccaaaag 24540
atcataaaga gcaagacgag cacgacgcag acgcacacca gggtgaagtc gggcgggggg 24600
acggagggca tggcggcgcc gactacctag acgaaggaaa cgacgtgctc ttgaagcacc 24660
tgcatcgtca gtgcgccatt gtctgcgacg ctctgcagga gcgcagcgag gtgcccctca 24720
gcgtggcgga ggtcagccgc gcctacgagc tcagcctctt ttccccccgg gtgccccccc 24780
gccgccgcga aaacggcaca tgcgagccca acccgcgcct caacttctac cccgcctttg 24840
tggtgcccga ggtcctggcc acctatcaca tcttctttca aaattgcaag atccccatct 24900
cgtgccgcgc caaccgtagc cgcgccgata agatgctggc cctgcgccag ggcgaccaca 24960
tacctgatat cgccgctttg gaagatgtgc caaagatctt cgagggtctg ggtcgcaacg 25020
agaagcgggc agcaaactct ctgcaacagg aaaacagcga aaatgagagt cacactggag 25080
cgctggtgga gctggagggc gacaacgccc gcctggcggt gctcaagcgc agcatcgagg 25140
tcacccactt tgcctacccc gcgctcaacc tgccccccaa agtcatgaac gcggtcatgg 25200
acgggctaat catgcgccgc ggccggcccc ttgctccaga tgcaaacttg catgaggaga 25260
ccgaggacgg tcagcccgtg gtcagcgacg agcagctgac gcgctggctg gaaaccgcgg 25320
accccgccga actggaggag cggcgcaaga tgatgatggc cgcggtgctg gtcaccgtag 25380
agctggagtg tctgcagcgc ttcttcggcg accccgagat gcagagaaag gtcgaggaga 25440
ccctacacta caccttccgc cagggctacg tgcgccaggc ttgcaagatc tccaacgtgg 25500
agctcagcaa cctggtgtcc tacctgggca tcttgcatga aaaccgcctt gggcagagcg 25560
tgctacactc caccctgcgc ggggaggcgc gccgcgacta cgtgcgcgac tgcgtttacc 25620
tcttcctctg ctacacctgg cagacggcca tgggggtctg gcagcagtgc ctggaggagc 25680
gcaacctcaa ggagctggag aagctcctgc agcgcgcgct caaagacctc tggacgggct 25740
acaacgagcg ctcggtggcc gccgcgctgg ccgacctcat cttccccgag cgcctgctca 25800
aaactctcca gcaggggctg cccgacttca ccagccaaag catgttgcaa aattttagga 25860
actttatcct ggagcgttct ggcatcctac ccgccacctg ctgcgccctg cccagcgact 25920
ttgtccccct cgtgtaccgc gagtgccccc cgccgctgtg gggccactgc tacctgttcc 25980
aactggccaa ctacctgtcc taccacgcgg acctcatgga agactccagc ggcgaggggc 26040
tcatggagtg ccactgccgc tgcaacctct gcacgcccca ccgctccctg gtctgcaaca 26100
cccaactgct cagcgagagt cagattatcg gtaccttcga gctacagggt ccgtcctcct 26160
cagacgagaa gtccgcggct ccggggctaa aactcactcc ggggctgtgg acttccgcct 26220
acctgcgcaa atttgtacct gaagactacc acgcccacga gatcaggttt tacgaggacc 26280
aatcccgccc gcccaaggcg gagctgaccg cctgcgtcat cacccagggc gagatcctag 26340
gccaattgca agccatccaa aaagcccgcc aagagttttt gctgagaaag ggtcgggggg 26400
tgtatctgga cccccagtcg ggtgaggagc tcaacccggt tcccccgctg ccgccgccgc 26460
gggaccttgc ttcccaggat aagcatcgcc atggctccca gaaagaagca gcagcggccg 26520
ccactgccgc caccccacac gctggaggaa gaggaggaat actgggacag tcaggcagag 26580
gaggtttcgg acgaggagga gccggagacg gagatggaag agtgggagga ggacagctta 26640
gacgaggagg cttccgaagc cgaagaggca ggcgcaacac cgtcaccctc ggccgcagcc 26700
ccctcgcagg cgcccccgaa gtccgctccc agcatcagca gcaacagcag cgctataacc 26760
tccgctcctc caccgccgcg acccacggcc gaccgcagac ccaaccgtag atgggacacc 26820
accggaaccg gggccggtaa gtcctccggg aaaggcaagc aagcgcagcg ccaaggctac 26880
cgctcgtggc gcgctcacaa gaacgccata gtcgcttgct tgcaagactg cggggggaac 26940
atctccttcg cccgccgctt cctgctcttc caccacggtg tggccttccc ccgtaacgtc 27000
ctgcattact accgtcatct ctacagcccc tactgcggcg gcagtgagcc agaggcggcc 27060
ggcggcagcg gcgcccgttt cggtgcctag gaagacccag ggcaagactt cagccaagaa 27120
actcgcggcg gccgcggcga acgcggtcgc gggggccctg cgcctgacgg tgaacgaacc 27180
cctgtcgacc cgcgaactga ggaaccgaat cttccccact ctctatgcca tcttccagca 27240
gagcagaggg caggatcagg aactgaaagt aaaaaacagg tctctgcgct ccctcacccg 27300
cagctgtctg tatcacaaga gcgaagacca gcttcggcgc acgctggagg acgctgaggc 27360
actcttcagc aaatactgcg cgctcactct taaggactag ctccgcgccc ttctcgaatt 27420
taggcgggaa cgcctacgtc atcgcagcgc cgccgtcatg agcaaggaca ttcccacgcc 27480
atacatgtgg agctatcagc cgcagatggg actcgcggcg ggcgcctccc aggattactc 27540
cacccgcatg aactggctca gtgccggccc acacatgatc tcacaggtta atgacatccg 27600
cacccatcga aaccaaatat tggtggagca ggcggcaatt accaccacgc cccgcaataa 27660
tcccaacccc agggagtggc ccgcgtccct ggtgtatcag gaaattcccg gccccaccac 27720
cgtactactt ccgcgtgatt cccaggccga agtccaaatg actaactcag gggcacagct 27780
cgcgggcggc tgtcgtcaca gggtgcggcc tcctcgccag ggtataactc acctgaagat 27840
ccgaggcaga ggtattcagc tcaacgacga gtcggtgagc tcctcgctcg gtctcagacc 27900
tgacgggacc ttccagatag ccggagccgg ccgatcttcc ttcacgcccc gccaggcgta 27960
cctgactctg cagagctcgt cctcggcgcc gcgctcgggc ggcatcggga ctctccagtt 28020
cgtgcaggag tttgtgccct cggtctactt caaccccttc tcgggctctc ccggtcgcta 28080
cccggaccag ttcatcccga actttgacgc cgcgagggac tcggtggacg gctacgactg 28140
aatgtcgggt ggacccggtg cagagcaact tcgcctgaag caccttgacc actgccgccg 28200
ccctcagtgc tttgcccgct gtcagaccgg tgagttccag tacttttccc tgcccgactc 28260
gcacccggac ggcccggcgc acggggtgcg ctttttcatc ccgagtcagg tccgctctac 28320
cctaatcagg gagttcaccg cccgtcccct actggcggag ttggaaaagg ggccttctat 28380
cctaaccatt gcctgcatct gctctaaccc tggattacac caagatcttt gctgtcattt 28440
gtgtgctgag tataataaag gctgagatca gaatctactc gggctcctgt cgccatcctg 28500
tcaacgccac cgtccaagcc cggcccgatc agcccgaggt gaacctcacc tgcggtctgc 28560
accggcgcct gaggaaatac ctagcttggt actacaacag cactcccttt gtggtttaca 28620
acagctttga ccaggacggg gtctcactga gggataacct ctcgaacctg agctactcca 28680
tcaggaagaa caacaccctc gagctacttc ctccttacct gcccgggact taccagtgtg 28740
tcaccggtcc ctgcacccac acccacctgt tgatcgtaaa cgactctctt ccgagaacag 28800
acctcaataa ctcctctccg cagttcccca gaacaggagg tgagctcagg aaaccccggg 28860
taaagaaggg tggacaagag ttaacacttg tggggtttct ggtgtatgtg acgctggtgg 28920
tggctctttt gattaaggct tttccttcca tgtctgaact atccctcttc ttttatgaac 28980
aactcgacta gtgctaacgg gaccctaccc aacgaatcgg gattgaatat cggtaaccag 29040
gttgcagttt cacttttgat taccttcata gtcctcttcc tgctagtgct gtcgcttctg 29100
tgcctgcgga tcgggggctg ctgcatccac gtttatatct ggtgctggct gtttagaagg 29160
ttcggagacc accgcaggta gaataaacat gctactgctc gccttcctta tcctggcgct 29220
ggccgcccaa tgccaagcct tttccgaggc tgactttgta gagccccagt gcaacgttac 29280
ttttagagcc tatgcacagc gttgtcatac tataatcaaa tgtgccaccg aacacaatgg 29340
ataccttatt cagtataaag ataaatcaca caaagtggca cttgttgaca tctgggaacc 29400
cgaagaccct ctggaataca atgttaccgt tttccagggt gacctcttca gaacttacaa 29460
ttacactttc ccatttgacc agatgtgtga ctttgtcatg tacatggaaa agcagcacaa 29520
gctgtggcct ccaactcccc agggctgtgt ggaaaatcca ggctctttct gtatgatctc 29580
tctctgtgta actgtgctgg cactaatact cacgcttctg tatctcagat ttaaatcaag 29640
gcaaagcttt attgatgaaa agaaaatgcc ttaatcgctt tcacgcttga ttgctaacac 29700
cgggttttta tccgcagaat gattggaatc accctactaa tcacctccct ccttgcgatt 29760
gcccatgggt tggaacgaat cgaagtccct gtgggggcca atgttaccct ggtggggcct 29820
gtcggcaatg ctacattaat gtgggaaaaa tatactaaaa atcaatgggt ctcttactgc 29880
actaacaaaa acagccacaa gcccagagcc atctgcgatg ggcaaaattt aaccttgatt 29940
gatgttcaat tgctggatgc gggctactat tatgggcagc tgggtacaat gattaattac 30000
tggagacccc acagagatta catgcttcac gtagtaaagg gtcccattaa cagcccaacc 30060
accacctcta ccacccccac taccaccact actcccacca ccagcactgc cgcccagcct 30120
cctcatagca gaacaaccac ttttatcaat tccaagtccc actcccccca cattgccggc 30180
gggccctccg cctcagactc cgagactacc gagatctgct tctgcaaatg ctctgacgcc 30240
attgcccagg atttggaaga tcacaaggaa gatgagcatg actacgcaga tgcatgccag 30300
gcatcagagt cagaagcgct gccggtggcc ctaaaacagt atgcagaccc ccacaccacc 30360
cccgaccttc ctccaccttc ccagaagcca agtttcctgg gggaaaatga aactctgcct 30420
ctctccatac tagctctgac atctgttgct attttggccg ctctgctggt gcttctatgc 30480
tctatatgct acctgatctg ctgcagaaag aaaaaatctc acggccatgc tcaccagccc 30540
ctcatgcact tcccttaccc tccagagctg ggcgaccaca aactttaagt ctgcagtagc 30600
tatctgccca tcccttgtca gtcgacagcg atgagcccca ctaatctaac agcctctgga 30660
cttacaacat tgtctcttaa tgagaccacc gctcctcaag acctgtacga tggtgtctcc 30720
gcgctggtta accagtggga tcacctgggc atatggtggc tcctcatagg agcagtgacc 30780
ctgtgcctaa tcctggtctg gatcatctgc tgcatcaaaa gcagaagacc caggcggcgg 30840
cccatctaca ggcccttcgt catcacacct gaagatgatg atgatgacac cacctccagg 30900
ctgcagagcc taaagcggct actcttctct tttacagcat ggtaaattga atcatgcccc 30960
gcattttcat ctacttgctt ctccttccac tttttctggg ctcctctaca ttggccgctg 31020
tgtcccacat cgaggtagac tgcctcacgc ccttcacagt ctacctgctt ttcggctttg 31080
tcatctgcac ctttgtctgc agcgttatca ctgtagtgat ctgcttcata cagtgcatcg 31140
actacatctg tgtgcgggtg gcctacttta gacaccaccc ccagtatcgc aacagggaca 31200
tagcggctct cctaagactt gtttaaatca tggccaaatt acctgtgatt ggtcttctga 31260
ttatctgctg cgtcctagcc gcgattggga ctcaacctaa taccaccacc agcgctccca 31320
gaaagagaca tgtatcctgc agcttcaagc gtccctggaa tataccccaa tgctttactg 31380
atgaacctga aatctctttg gcttggtact tcagcgtcac cgcccttctc atcttctgca 31440
gtacggttat tgctcttgcc atctaccctt cccttaacct gggctggaat gctgtcaact 31500
ctatggaata tcccaccttc ccagaaccag acctgccaga cctggttgtt ctaaacgcgt 31560
ttcctcctcc tccagttcaa aatcagtttc gccctccgtc ccctacgccc actgaggtca 31620
gctactttaa tctaacaggc ggagatgact gaaaacctag acctagaaat ggacggtctc 31680
tgcagcgagc aacgcatact agagaggcgc cggcaaaaag cagagctcga gcgtcttaaa 31740
caagagctcc aagacgccgt ggccatacac cagtgcaaaa aagggctctt ctgtctggta 31800
aaacaggcca cgctcaccta tgaaaaaaca ggtgacaccc accgcctagg atacaagctg 31860
cccacacagc gccaaaagtt tgcccttatg ataggtgaac aacccatcac cgtgacccag 31920
cactccgtgg agacagaagg ctgcattcat gctccctgca ggggcgctga ctgcctctac 31980
accttgatca aaaccctttg cggtctcaga gaccttatcc ctttcaattg atcataactg 32040
taatcaataa aaaatcactt acttgaaatc tgatagcaag cctctgtcca attttttcag 32100
caacacttcc ttcccctctt cccaactctg gtactctagg cgcctcctag ctgcaaactt 32160
cctccacagt ctgaagggaa tgtcagattc ctcctcctcc tgtccctccg cacccacgat 32220
cttcatgttg ttgcagatga aacgcgcaag atcgtctgac gagaccttca accccgtgta 32280
cccctacgat accgagatcg ctccgacttc tgtccctttc cttacccctc cctttgtgtc 32340
atccgcagga atgcaagaaa atccagctgg ggtgctgtcc ctgcacctgt cagagcccct 32400
taccacccac aatggggccc taactctaaa aatggggggc ggcctaaccc tggacaagga 32460
agggaatctc acttcccaaa acatcaccag tgtcgatccc cctctcaaaa aaagcaaaaa 32520
caacatcagc cttcagaccg ccgcacccct cgccgtcagc tccggggccc taaccctttt 32580
tgccactccc cccctagcgg tcagtggcga caaccttact gtgcagtctc aggccccttt 32640
cactttggaa gactcaaaac taactctggc caccaaagga cccctaactg tgtccgaagg 32700
caaacttgtc ctagaaacag aggctcccct gcatgcaagt gacagcagta gcctgggcct 32760
tagcgtcacg gccccactta gcattaacaa tgacagccta ggactagaca tgcaagcgcc 32820
catcagctct cgagatggaa aactggctct aacagtggcg gcccccctaa ctgtggccga 32880
gggtatcaat gctttggcag tagccacagg taatggtatt ggactaaatg aaaccaacac 32940
acacctgcag gcaaaactgg tcgcgcccct aggctttgat accaacggca acattaagct 33000
aagcgtcgca ggaggcatga ggctaaacaa taacacactg atactagatg taaactaccc 33060
atttgaggct caaggccaac tgagcctaag agtgggctcg ggcccactat atgtagattc 33120
tagtagtcat aacctaacca ttagatgcct taggggattg tatgtaacat cttctaacaa 33180
ccaaaacggt ctagaggcca acattaaact aacaaaaggc cttgtgtatg acggaaatgc 33240
catagcagtt aatgttggca aagggctgga atacagccct actggcacaa cagaaaaacc 33300
tatacagact aaaataggtc taggcatgga gtatgacact gagggagcca tgatgacaaa 33360
actaggctct ggactaagct ttgacaattc aggagccatt gtggtgggaa acaaaaatga 33420
tgacaggctt actttgtgga ccacaccgga cccatcgccc aactgtcaga tttactctga 33480
aaaagatgct aaactaacct tggtactgac taaatgtggc agtcaggttg taggcacagt 33540
atctatcgcc gctcttaaag gtagccttgt gccaatcact agtgcaatca gtgtggttca 33600
ggtataccta aggtttgatg aaaatggggt actgatgagt aactcttcac ttaatggcga 33660
atactggaat tttagaaacg gagactcaac taatggcaca ccatatacaa acgcagtggg 33720
ttttatgcct aatctactgg cctatcctaa aggtcaaact acaactgcaa aaagtaacat 33780
tgtcagccag gtttacatga atggggacga tactaaaccc atgacattta caatcaactt 33840
caatggcctt agtgaaacag gggatacccc tgtcagtaaa tattccatga cattctcatg 33900
gaggtggcca aatggaagct acatagggca caattttgta acaaactcct ttactttctc 33960
ctacatcgcc caagaataaa gaaagcacag agatgcttgt ttttgatttc aaaattgtgt 34020
gcttttattt attttcaagc ttacagtatt tccagtagtc attcgaatag agcttaatga 34080
aactgcatga gaacccttcc acatagctta aattatcacc agtgcaaatg gagaaaaatc 34140
aacatacctt tttatccaga tatcatagaa ctctagtggt cagttttccc ccaccctccc 34200
aactcacaga atacacagtc ctttcccccc ggctggcttt aaacaacact atctcattgg 34260
taacagacat attcttaggt gtaataatcc acacggtctc ttggcgggcc aaacgctggt 34320
cagtgatgtt aataaactcc ccaggcagct ctttcaagtt cacgtcgctg tccaactgct 34380
gaagcgctcg cggctccgac tgcgcctcta gcggaggcaa cggcaacacc cgatccttga 34440
tctataaagg agtagagtca taatccccca taagaatagg gcggtgatgc tgcaacaagg 34500
cgcgcagcaa ctcctgccgc cgcctctccg tacgacagga atgcaacggg gtggtggtct 34560
cctccgcgat aatccgcacc gctcgcagca tcagcgtcct cgtcctccgg gcacagcagc 34620
gcattctgat ctcactgaga tcggcgcagt aagtgcagca caacaccaag atgttattta 34680
agatcccaca gtgcaaagca ctgtacccaa agctcatggc gggaaggaca gcccccacgt 34740
gaccatcata ccagatcctc aggtaaatca aatgacgacc tctcatgaac acgctggaca 34800
tgtacatcac ctccttgggc atgtgctgat tcaccacctc tcgataccac aggcatcgct 34860
gattaattaa agacccctcg agcaccatcc tgaaccagga agccagcacc tgaccccccg 34920
ccaggcactg cagggacccc ggtgaatcgc agtggcagtg aagactccag cgctcgtagc 34980
cgtgaaccat agagctggtc attatatcca cattggcaca acacagacac actttcatac 35040
actttttcat gattagcagc tcctctctag tcaggaccat atcccaagga atcacccact 35100
cttgaatcaa ggtaaatccc acacagcagg gcaggcctct cacataactc acgttatgca 35160
tagtaagcgt gtcgcaatct ggaaataccg gatgatcttc catcaccgaa gcccgggtct 35220
ccgtctcaaa gggaggtaaa cggtccctcg tgtagggaca gtggcgggat aatcgagatc 35280
gtgttgaacg tagaatcatg ccaaagggaa cagcggacgt actcatattt cctccagcag 35340
aaccaagtgc gcgcgtggca gctatccttg cgtcttctgt ctcgccgcct gccccgctcg 35400
gtgtagtagt tgtaatacag ccactccctc agaccgtcaa ggcgctccct ggcgtccgga 35460
tctataacaa caccatcctg cagcgccgcc ctgatgacat ccaccaccgt agagtatgcc 35520
aagcccagcc aggaaatgca ctcactttga cagcgagaga taggaggagc gggaagagat 35580
ggaagaacca tgatagtaaa agaactttta ttccaatcga tcctctacaa tgtcaaagtg 35640
tagatctatc agatggcact ggtctcctcc gctgagtcga tcaaaaataa cagctaaacc 35700
acaaacaaca cgattggtca aatgctgcac aagggcttgc agcataaaat cgcctcgaaa 35760
gtccaccgca agcataacat caaagccacc gcccctatca tgatctatga taaaaacccc 35820
acagctatcc accagaccca tatagttttc atctctccat cgtgaaaaaa tatttacaag 35880
ctcctccttt aaatcacctc caaccaattc aaaaagttga gccagaccgc cctccacctt 35940
cattttcagc atgcgcatca tgattgcaaa aattcaggct cctcagacac ctgtataaga 36000
ttgagaagcg gaacgttaac atcaatgttt cgctcgcgaa gatcgcgcct cagtgcaagc 36060
atgatataat cccacaggtc ggagcggatc agcgaggaca tctccccgcc aggaaccaac 36120
tcaacggagc ctatgctgat tataatacgc atattcgggg ctatgctaac cagcacggcc 36180
cccaaatagg cgtactgcat aggcggcgac aaaaagtgaa cagtttgggt taaaaaatca 36240
ggcaaacact cgcgcaaaaa agcaagaaca tcataaccat gctcatgcaa atagatgcaa 36300
gtaagctcag gaacgaccac agaaaaatgc acaatttttc tctcaaacat gactgcgagc 36360
cctgcaaaaa ataaaaaaga aacattacac aagagtagcc tgtcttacaa tgggatagac 36420
tactctaacc aacataagac gggccacgac atcgcccgcg tggccataaa aaaaattatc 36480
cgtgtgatta aaaagaagca cagatagctg gccagtcata tccggagtca tcacgtgcga 36540
acccgtgtag acccccgggt tggacacatc ggccaaagaa agaaagcggc caatgtatcc 36600
cggaggaatg ataacactaa gacgaagata caacagaata accccatggg ggggaataac 36660
aaagttagta ggtgaataaa aacgataaac acccgaaact ccctcctgcg taggcaaaat 36720
agcgccctcc ccttccaaaa caacatacag cgcttccaca gcagccatga caaaagactc 36780
aaaacactca aaagactcag tcttaccagg aaaataaaag cactctcaca gcaccagcac 36840
taatcagagt gtgaagaggg ccaagtgccg aacgagtata tataggaatt aaaaatgacg 36900
taaatgtgta aaggtcaaaa aacgcccaga aaaatacaca gaccaacgcc cgaaacgaaa 36960
acccgcgaaa aaatacccag aagttcctca acaaccgcca cttccgcttt cccacgatac 37020
gtcacttcct cgaaaatagc aaactacatt tcccacatgt acaaaaccga aaccactccc 37080
cttgtcaccg cccacaactt acatcattac aaacgtcaaa gcctacgtca gccgccccgc 37140
ctcgccccgc ccacctcatt atcatattgg ccacaatcca aaataaggta tattattgat 37200
gatg 37204
<210> 7
<211> 1164
<212> DNA
<213> 人工序列
<220>
<223> HAdV-5 E4 orf 6/7
<400> 7
tcacagaacc ctagtattca acctgccacc tccctcccaa cacacagagt acacagtcct 60
ttctccccgg ctggccttaa aaagcatcat atcatgggta acagacatat tcttaggtgt 120
tatattccac acggtttcct gtcgagccaa acgctcatca gtgatattaa taaactcccc 180
gggcagctca cttaagttca tgtcgctgtc cagctgctga gccacaggct gctgtccaac 240
ttgcggttgc ttaacgggcg gcgaaggaga agtccacgcc tacatggggg tagagtcata 300
atcgtgcatc aggatagggc ggtggtgctg cagcagcgcg cgaataaact gctgccgccg 360
ccgctccgtc ctgcaggaat acaacatggc agtggtctcc tcagcgatga ttcgcaccgc 420
ccgcagcata aggcgccttg tcctccgggc acagcagcgc accctgatct cacttaaatc 480
agcacagtaa ctgcagcaca gcaccacaat attgttcaaa atcccacagt gcaaggcgct 540
gtatccaaag ctcatggcgg ggaccacaga acccacgtgg ccatcatacc acaagcgcag 600
gtagattaag tggcgacccc tcataaacac gctggacata aacattacct cttttggcat 660
gttgtaattc accacctccc ggtaccatat aaacctctga ttaaacatgg cgccatccac 720
caccatccta aaccagctgg ccaaaacctg cccgccggct atacactgca gggaaccggg 780
actggaacaa tgacagtgga gagcccagga ctcgtaacca tggatcatca tgctcgtcat 840
gatatcaatg ttggcacaac acaggcacac gtgcatacac ttcctcagga ttacaagctc 900
ctcccgcgtt agaaccatat cccagggaac aacccattcc tgaatcagcg taaatcccac 960
actgcaggga agacctcgca cgtaactcac gttgtgcatt gtcaaagtgt tacattcggg 1020
cagcagcgga tgatcctcca gtatggtagc gcgggtttct gtctcaaaag gaggtagacg 1080
atccctactg tacggagtgc gccgagacaa ccgagatcgt gttggtcgta gtgtcatgcc 1140
aaatggaacg ccggacgtag tcat 1164
<210> 8
<211> 99
<212> DNA
<213> 人工序列
<220>
<223> HAdV-5蛋白IX启动子
<400> 8
ggtactgaaa tgtgtgggcg tggcttaagg gtgggaaaga atatataagg tgggggtctt 60
atgtagtttt gtatctgttt tgcagcagcc gccgccgcc 99
<210> 9
<211> 33893
<212> DNA
<213> 人工序列
<220>
<223> pBZ1_BZ28F
<400> 9
catcatcaat aatatacctt attttggatt gtggccaata tgataatgag gtgggcgggg 60
cgaggcgggg cgggtgacgt aggacgtgcg agtagggttg ggaggtgtgg cggaagtgtg 120
gcatttgtaa gtgggaggag cttacatgca agcttccgtc gcggaaaatg tgacgttttt 180
tatgagcgcc gcctacctcc ggaagtgcca attttcgcgc gctttttacc ggatatcgta 240
gtaattttgg gcgggaccat gtaagatttg gccattttcg cgcgaaaagt gaaacgggga 300
agtgaaaact gaataatagg gcgttagtca tagcgcgtaa tatttaccga gggccgaggg 360
actttgaccg attacgtgga ggactcgccc aggtgttttt tacgtgaatt tccgcgttcc 420
gggtcaaagt ctccgttttt attgtcaccg tcatttgacg cggagggtat ttaaacccgc 480
tgcgctccta gcgatcgctg atgagaccag gaccaggtgc cgaccctgcg agtgcggcgg 540
caagcacatg agaaatcagc ctgtgatgtt ggatgtgacc gaggagctta ggcctgacca 600
tctggtgctg gcctgcacca gggccgagtt tgggtctagc gatgaggata ccgattgagg 660
tgggtaaggt gggcgtggct agcagggtgg gcgtgtataa attgggggtc taaggggtct 720
ctctgtttgt cttgcaacag ccgccgccat gagcgacacc ggcaacagct ttgatggaag 780
catctttagc ccctatctga cagtgcgcat gcctcactgg gccggagtgc gtcagaatgt 840
gatgggttcc aacgtggatg gacgtcccgt tctgccttca aattcgtcta cgatggccta 900
cgcgaccgtg ggaggaactc cgctggacgc cgcgacctcc gccgccgcct ccgccgccgc 960
cgcgaccgcg cgcagcatgg ctacggacct ttacagctct ttggtggcga gcagcgcggc 1020
ctctcgcgcg tctgctcggg atgaaaaact gactgctctg ctgcttaaac tggaagactt 1080
gacccgggag ctgggtcaac tgacccagca ggtctccagc ttgcgtgaga gcagccttgc 1140
ctccccctaa tggcccataa tataaataaa agccagtctg tttggattaa gcaagtgtat 1200
gttctttatt taactctccg cgcgcggtaa gcccgggacc agcggtctcg gtcgtttagg 1260
gtgcggtgga ttctttccaa cacgtggtac aggtggctct ggatgtttag atacatgggc 1320
atgagtccat ccctggggtg gaggtagcac cactgcagag cttcgtgctc gggggtggtg 1380
ttgtatatga tccagtcgta gcaggagcgc tgggcgtggt gctgaaaaat gtccttaagc 1440
aagaggctta tagctagggg gaggcccttg gtgtaagtgt ttacaaatct gcttagctgg 1500
gaggggtgca tccgggggga tatgatgtgc atcttggact ggatttttag gttggctatg 1560
ttcccaccca gatcccttct gggattcatg ttgtgcagga ccaccagcac ggtatatcca 1620
gtgcacttgg gaaatttatc gtggagctta gacgggaatg catggaagaa cttggagacg 1680
cccttgtggc ctcccagatt ttccatacat tcgtccatga tgatggcaat gggcccgtgg 1740
gaagctgcct gagcaaaaac gtttctggca tcgctcacat cgtagttatg ttccagggtg 1800
aggtcatcat aggacatctt tacaaatcgg gggcggaggg tcccggactg ggggatgatg 1860
gtaccctcgg gccccggggc gtagttcccc tcacagatct gcatctccca ggctttcatt 1920
tcagagggag ggatcatatc cacctgcggg gcgatgaaaa agacagtttc tggcgcaggg 1980
gagattaact gggatgagag caggtttctg agcagctgtg actttccaca gccggtgggc 2040
ccatatatca cgcctatcac cggctgcagc tggtagttaa gagagctgca gctgccgtcc 2100
tcccggagca ggggggccac ctcgttgagc atatccctga cgtggatgtt ctccctgacc 2160
agttccgcca gaaggcgctc gccgcccagc gaaagcagct cttgcaagga agcaaaattt 2220
ttcagcggtt tcaggccatc ggccgtgggc atgtttttca gcgtctgggt cagcagctcc 2280
agcctgtccc agagctcggt gatgtgctct acggcatctc gatccagcag atctcctcgt 2340
ttcgcgggtt ggggcggctt tcgctgtagg gcaccagccg atgggcgtcc agcggggcca 2400
gagtcatgtc cttccatggg cgcagagtcc tcgtcagggt ggtctgggtc acggtgaagg 2460
ggtgcgctcc gggttgggca ctggccaggg tgcgcttgag gctggttctg ctggtgctga 2520
atcgctgccg ctcttcgccc tgcgcgtcgg ccaggtagca tttgaccatg gtctcgtagt 2580
cgagaccctc ggcggcgtgc cccttggcgc ggagctttcc cttggaggtg gcgccgcacg 2640
aggggcactg caggctcttc agggcgtaga gcttgggagc gagaaacacg gactctgggg 2700
agtaggcgtc cgcgccgcag gccgcgcaga ccgtctcgca ttccaccagc caagtgagtt 2760
ccgggcggtc agggtcaaaa accaggttgc ccccatgctt tttgatgcgt ttcttacctc 2820
ggctctccat gaggcggtgt cccttctcgg tgacgaagag gctgtccgtg tccccgtaga 2880
ccgacttcag gggcctgtct tccagcggag tgcctctgtc ctcctcgtag agaaactctg 2940
accactctga gacgaaggcc cgtgtccagg ccaggacgaa ggaggccacg tgggaggggt 3000
agcggtcgtt gtccactagc gggtccacct tctccagggt gtgcagacac atgtccccct 3060
cctccgcgtc cagaaaagtg attggcttat aggtgtagga cacgtgaccg ggggttcccg 3120
acgggggggt ataaaagggg gtgggcgccc tttcatcttc actctcttcc gcatcgctgt 3180
ctgcgagagc cagctgctgg ggtaagtatt ccctttcaaa ggcgggcatg acctcagcgc 3240
tcaggttgtc agtttctaaa aatgaggagg atttgatgtt cacctgtccg gaagtgatac 3300
ctttgagggt acctgggtcc atctggtcag aaaacactat ttttttgttg tcaagcttgg 3360
tggcgaacga cccgtagagg gcgttggaga gcagcttggc aatggagcgc agggtctggt 3420
ttttgtcgcg gtcggctcgc tccttggccg cgatgttgag ttgcacgtat tcgcgggcca 3480
cgcacttcca ctcggggaag acggtggtgc gctcgtctgg gatcaggcgc accctccagc 3540
cgcggttgtg cagggtgacc atgtcgacgc tggtggcgac ctcaccgcgc agacgctcgt 3600
tggtccagca gaggcggccg cctttgcgcg agcagaaggg gggtaggggg tccagctggt 3660
cctcgtttgg ggggtccgcg tcgatggtaa agaccccggg gagcaggcgc gggtcaaagt 3720
agtcgatctt gcaagcttgc atgtccagag cccgctgcca ttcgcgggcg gcgagcgcgc 3780
gctcgtaggg gttgaggggc gggccccagg gcatggggtg ggtgagcgcg gaggcgtaca 3840
tgccgcagat gtcatacacg tacaggggtt ccctgaggat accgaggtag gtggggtagc 3900
agcgcccccc gcggatgctg gcgcgcacgt agtcatagag ctcgtgggag ggggccagca 3960
tgttgagccc aaggttggtg cgctgggggc gctcggcgcg gaagacgatc tgtctgaaga 4020
tggcatggga gttggaggag atggtgggtc gctggaagac gttgaagctt gcttcttgca 4080
agcccacgga gtccctgacg aaggaggcgt aggactcgcg cagcttgtgc accagctcgg 4140
cggtgacctg gacgtcgagc gcgcagtagt cgagggtctc gcggatgatg tcatacttat 4200
cctccccctt ctttttccac agctcgcggt tgaggacgaa ctcttcgcgg tctttccagt 4260
actcttggag gggaaacccg tccgtgtccg aacggtaaga gcctagcatg tagaactggt 4320
tgacggcctg gtaggggcag cagcccttct ccacgggcag cgcgtaggcc tgcgccgcct 4380
tgcggaggga ggtgtgggta agggcgaaag tgtccctgac catgactttg aggtattgat 4440
gtctgaagtc tgtgtcatcg cagccgccct gttcccacag ggtgtagtcc gtgcgctttt 4500
tggagcgcgg gttgggcagg gagaaggtga ggtcattgaa gaggatcttc cccgctcgag 4560
gcatgaagtt tctggtgatg cgaaagggcc ctgggaccga ggagcggttg ttgatgacct 4620
gggcggccag gacgatctcg tcaaagccgt ttatgttgtg gcccacgatg tagagctcca 4680
ggaagcgggg ctggcccttg atggagggga gctttttaag ttcctcgtag gtaagctcct 4740
cgggcgattc caggccgtgc tcctccaggg cccagtcttg caagtgaggg ttggccgcca 4800
ggaaggatcg ccagaggtcg cgggccatga gggtctgcag gcggtcgcgg aaggttctga 4860
actgccgccc tacggccatc ttttcggggg tgatgcagta gaaggtgagg gggtctttct 4920
cccaggggtc ccatctgagc tcttgggcga ggtcgcgcgc ggcggcgacc agagcctcgt 4980
cgccccccag tttcatgacc agcatgaagg gcacgagctg cttgccaaag gctcccatcc 5040
aagtgtaggt ctctacatcg taggtgacaa agaggcgctc cgtgcgagga tgagagccga 5100
tcgggaagaa ctggatctcc cgccaccagt tggaggattg gctgttgatg tggtgaaagt 5160
agaagtcccg tctgcgggcc gagcactcgt gctggctttt gtaaaagcga ccgcagtact 5220
ggcagcgctg cacgggttgt atatcttgca cgaggtgaac ctggcgacct ctgacgagga 5280
agcgcagcgg gaatctaagt cccccgcctg gggtcccgtg tggctggtgg tcttctactt 5340
tggttgtctg gccgccagca tctgtctcct ggagggcgat ggtggagcag accaccacgc 5400
cgcgagagcc gcaggtccag atctcggcgc tcggcgggcg gagtttgatg acgacatcgc 5460
gcacattgga gctgtccatg gtctccagct cccgcggcgg caggtcagcc gggagttcct 5520
ggaggttcac ctcgcagaga cgggtcaagg cgcggacagt gttgagatgg tatctgattt 5580
caaggggcgt gttggaggcg gagtcgatgg cttgcagaag gccgcagccc cggggggcca 5640
cgatggttcc ccgcggggcg cgaggggagg cggaagctgg gggtgtgttc agaagcggtg 5700
acgcgggcgg gcccccggag gtaggggggg ttccggcccc acaggcatgg gcggcagggg 5760
cacgtcttcg ccgcgcgcgg gcaggggctg gtgctggctc cggagagcgc ttgcgtgcgc 5820
gacgacgcga cggttggtgt cctgtatctg gcgcctctga gtgaagacca cgggtcccgt 5880
gaccttgaac ctgaaagaga gttcgacaga atcaatctcg gcatcgttga cagcggcctg 5940
gcgcaggatc tcctgcacgt cgcccgagtt gtcctggtag gcgatctctg ccatgaactg 6000
ctcgatctct tcctcctgga gatctcctcg tccggcgcgc tccacggtgg ccgccaggtc 6060
gttggagatg cgacccatga gctgcgagaa ggcgttgagg ccgccctcgt tccagacccg 6120
gctgtagacc acgcccccct cggcgtcgcg ggcgcgcatg accacctggg ccaggttgag 6180
ctccacgtgt cgcgtgaaga cggcgtagtt gcgcaggcgc tggaaaaggt agttcagggt 6240
ggtggcggtg tgttcggcga cgaagaagta catgacccag cgccgcaacg tggattcatt 6300
gatgtccccc aaggcctcca ggcgctccat ggcctcgtag aagtccacgg cgaagttgaa 6360
aaactgggag ttgcgagcgg acacggtcaa ctcctcctcc agaagacgga tgagctcggc 6420
gacagtgtcg cgcacctcgc gctcgaaggc cacggggggc gcttcttcct cttccacctc 6480
ttcttccatg attgcttctt cttcctcagc cgggacggga gggggcggcg gcgggggagg 6540
ggcgcggcgg cggcggcggc gcaccggcag gcggtcgatg aagcgctcga tcatctcccc 6600
ccgcatgcgg cgcatggtct cggtgacggc gcggccgttc tcccgggggc gcagctcaaa 6660
gacgccgcct ctcatctcgc cgcggggcgg gcggccgtga ggtagcgaga cggcgctgac 6720
tatgcatctt aacaattgct gtgtaggtac gccgccaagg gacctgattg agtccagatc 6780
caccggatcc gaaaaccttt ggaggaaagc gtctatccag tcgcagtcgc aaggtaggct 6840
gagcaccgtg gcgggcgggg gcgggtcggg agagttcctg gcggagatgc tgctgatgat 6900
gtaattaaag taggcggtct tgagaaggcg gatggtggac aggagcacca tgtctttggg 6960
tccggcctgt tggatgcgga ggcggtcggc catgccccag gcctcgttct gacaccggcg 7020
caggtctttg tagtagtctt gcatgagtct ttccaccggc acctcttctc cttcctcttc 7080
tccatctcgc cggtggtttc tcgcgccgcc catgcgcgtg accccaaagc ccctgagcgg 7140
ctgcagcagg gccaggtcgg cgaccacgcg ctcggccaag atggcctgct gtacctgagt 7200
gagggtcctc tcgaagtcat ccatgtccac gaagcggtgg taggcgcccg tgttgatggt 7260
gtaggtgcag ttggccatga cggaccagtt gacggtctgg tgtcccggct gcgagagctc 7320
cgtgtaccgc aggcgcgaga aggcgcggga atcgaacacg tagtcgttgc aagtccgcac 7380
cagatactgg tagcccacca ggaagtgcgg cggaggttgg cgatagaggg gccagcgctg 7440
ggtggcgggg gcgccgggcg ccaggtcttc cagcatgagg cggtggtatc cgtagatgta 7500
cctggacatc caggtgatgc cggcggcggt ggtggtggcg cgcgcgtagt cgcggacccg 7560
gttccagatg tttcgcaggg gcgagaagtg ttccatggtc ggcacgctct ggccggtgag 7620
gcgcgcgcag tcgttgacgc tctatacaca cacaaaaacg aaagcgttta cagggctttc 7680
gttctgtagc ctggaggaaa gtaaatgggt tgggttgcgg tgtgccccgg ttcgagacca 7740
agctgagctc ggccggctga agccgcagct aacgtggtat tggcagtccc gtctcgaccc 7800
aggccctgta tcctccagga tacggtcgag agcccttttg ctttcttggc caagcgcccg 7860
tggcgcgatc tgggatagat ggtcgcgatg agaggacaaa agcggctcgc ttccgtagtc 7920
tggagaaaca atcgccaggg ttgcgttgcg gcgtaccccg gttcgagccc ctatggcggc 7980
ttggatcggc cggaaccgcg gctaacgtgg gctgtggcag ccccgtcctc aggaccccgc 8040
cagccgactt ctccagttac gggagcgagc cccttttgtt ttttattttt tagatgcatc 8100
ccgtgctgcg gcagatgcgc ccctcgcccc ggcccgatca gcagcagcaa cagcaggcat 8160
gcagaccccc ctctcctcta cccgccccgg tcaccacggc cgcggcggcc gtgtccggcg 8220
cggggggcgc gctggagtca gatgagccac cgcggcggcg acctaggcag tatctggact 8280
tggaagaggg cgagggactg gcgcggctgg gggcgagctc tccagagcgc cacccgcggg 8340
tgcagttgaa aagggacgcg cgcgaggcgt acctgccgcg gcagaacctg tttcgcgacc 8400
gcgggggcga ggagcccgag gagatgcgag actgcaggtt ccaagcgggg cgcgagctgc 8460
gccgcgggtt ggacagacag cgcctgctgc gcgaggagga ctttgagccc gacacgcaga 8520
cgggcatcag ccccgcgcgc gcgcacgtgg ccgcggccga cctggtgacc gcctacgagc 8580
agacggtgaa ccaggagcgc aacttccaaa aaagcttcaa caaccacgtg cgcacgctgg 8640
tggcgcgcga ggaggtgacc ctgggtctca tgcatctgtg ggacctggtg gaggcgatcg 8700
tgcagaaccc cagcagcaag cccctgaccg cgcagctgtt cctggtggtg cagcacagca 8760
gggacaacga ggccttcagg gaggcgctgc tgaacatcac cgagccggag gggcgctggc 8820
tcctggacct gataaacatc ctgcagagca tagtggtgca ggagcgcagc ctgagcctgg 8880
ccgagaaggt ggcggccatt aactattcta tgctgagctt gggcaagttc tacgcccgca 8940
agatctacaa gaccccctac gtgcccatag acaaggaggt gaagatagac agcttctaca 9000
tgcgcatggc gctaaaggtg ctgaccctga gcgacgacct gggagtgtac cgcaacgagc 9060
gcatccacaa ggccgtgagc gccagccggc ggcgcgagct gagcgaccgc gagctgatgc 9120
acagtctgca gcgcgcgctc accggcgcgg gcgagggcga cagggaggtc gagtcctact 9180
tcgacatggg ggctgacctg cactggcagc cgagccgccg cgccctggag gcggcggggg 9240
cgtatggcgg ccccctggcg gccgatgacg aggaagagga ggactatgag ctagaggagg 9300
gcgagtacct ggaggactga cctggctggt ggtgttttgg tatagatgca agatccgaac 9360
gtggcggacc cggcggtccg ggcggcgctg cagagccagc cgtccggcat taactcctct 9420
gacgactggg ccgcggccat gggtcgcatc atggccctga ccgcgcgcaa ccccgaggcc 9480
ttcaggcagc agcctcaggc taaccggctg gcggccatct tggaagcggt agtgcccgcg 9540
cgctccaacc ccacccacga gaaggtgctg gccatagtca acgcgctggc ggagagcagg 9600
gccatccggg cggacgaggc cggactggtg tacgatgcgc tgctgcagcg ggtggcgcgg 9660
tacaacagcg gcaacgtgca gaccaacctg gaccgcctgg tgacggacgt gcgcgaggcc 9720
gtggcgcagc gcgagcgctt gcatcaggac ggtaacctgg gctcgctggt ggcgctaaac 9780
gccttcctca gcacccagcc ggccaacgta ccgcgggggc aggaggacta caccaacttc 9840
ttgagcgcgc tgcggctgat ggtgaccgag gtccctcaga gcgaggtgta ccagtcgggg 9900
cccgactact tcttccagac cagcagacag ggcttgcaaa ccgtgaacct gagccaggct 9960
ttcaagaacc tgcgggggct gtggggagtg aaagcgccca ccggcgaccg agctacggtg 10020
tccagcctgc taacccccaa ctcgcgcctg ctgctgctgc tgatcgcgcc cttcacggac 10080
agcgggagcg tctcgcggga gacctatctg ggccacctgc tgacgctgta ccgcgaggcc 10140
atcgggcagg cgcaggtgga cgagcacacc ttccaagaga tcaccagcgt gagccacgcg 10200
ctggggcagg aggacacggg cagcctgcag gcgaccctga actacctgct gaccaacagg 10260
cggcagaaga ttcccacgct gcacagcctg acccaggagg aggagcgcat cttgcgctac 10320
gtgcagcaga gcgtgagcct gaacctgatg cgcgacggcg tgacgcccag cgtggcgctg 10380
gacatgaccg cgcgcaacat ggaaccgggc atgtacgctt cccagcggcc gttcatcaac 10440
cgcctgatgg actacttgca tcgggcggcg gccgtgaacc ccgagtactt caccaatgcc 10500
attctgaatc cccactggat gccccctccg ggtttctaca acggagactt cgaggtgcct 10560
gaggtcaatg atgggttcct ctgggatgac atggatgaca gtgtgttctc ccccaacccg 10620
ctgcgcgccg cgtctctgcg attgaaggag ggctctgaca gggaaggacc gaggagtctg 10680
gcctcctccc tggctctggg ggcggtgggc gccacgggcg cggcggcgcg gggcagcagc 10740
cccttcccca gcctggcaga ctctctgaat agcgggcggg tgagcaggcc ccgcttgcta 10800
ggcgaggagg agtatctgaa caactccctg ctgcagcccg tgagggacaa aaacgctcag 10860
cgacagcagt ttcccaacaa cgggatagag agcctggtgg acaagatgtc cagatggaag 10920
acatatgcgc aggagtacaa ggagtgggag gaccgccagc cgcggcccct gccgccccct 10980
agacagcgct ggcagcggcg cgcgtccaac cgccgctgga gacagggacc cgaggacgat 11040
gatgactctg cagatgacag cagcgtgttg gacctgggcg ggagcgggaa ccccttttcg 11100
cacctgcgcc cacgcctggg caagatgttt taaaagagaa aaataaaact caccaaggcc 11160
atggcgacga gcgttggttt tttgttccct tccttagtat gcggcgcgcg gcgatgttcg 11220
aggaggggcc tcccccctct tacgagagcg cgatgggaat ttctcctgcg gcgcccctgc 11280
agcctcccta cgtgcctcct cggtacctgc aacctacagg ggggagaaat agcatctgtt 11340
actctgagct gcagcccctg tacgatacca ccagactgta cctggtggac aacaagtccg 11400
cggacgtggc ctccctgaac taccagaacg accacagcga ttttttgacc acggtgatcc 11460
aaaacaacga cttcacccca accgaggcca gtacccagac cataaacctg gacaacaggt 11520
cgaactgggg cggcgacctg aagaccatcc tgcacaccaa catgcccaac gtgaacgagt 11580
tcatgtttac caactctttt aaggcgcggg ttatggtggc gcgcgagcag ggggaggcga 11640
agtacgagtg ggtggacttc acgctgcccg agggcaacta ctcagagacc atgactattg 11700
acctgatgaa caatgcgatc gtggaacact acctgaaagt gggcaggcag aacggggtga 11760
aggagagcga tatcggggtc aagtttgaca ccagaaactt tcgtctgggc tgggaccccg 11820
tgaccgggct ggtcatgccg ggggtctaca ccaacgaggc ctttcatccc gatatagtgc 11880
tcctgcccgg ctgtggggtg gactttaccc agagccggct gagcaacctg ctgggcgttc 11940
gcaagcggca acctttccag gagggtttca agatcaccta tgaggatctg gaggggggca 12000
acattcccgc gctccttgat ctggacgcct acgaggagag cttgaaaccc gaggagagcg 12060
ctggcgacag cggcgagagt ggcgaggagc aagccggcgg cggtggcagc gcgtcggtag 12120
aaaacgaaag tactcccgca gtggcggcag acgctgcgga ggtcgagcca gaggccatgc 12180
agcaggacgc agaggagggc gcacaggagg gcgcgcagga ggacatgaac gatggggaga 12240
tcaggggaga cactttcgcc acccggggcg aagaaaaaga ggcagaggcg gtggcggcga 12300
cggtggaagc cgaaaccgag gcagaggcag agcccaagac cgaagttatg gaagacatga 12360
atgatggaga acgtaggggt gacacgtttg ccacccgggg cgaagagaag gcggcggagg 12420
cagaagccgc ggctgaggag gcggctgcgg ctgcggccga ggctgaggct gcggctgagg 12480
ctaaggtcga agccgatgtt gcggttgagg ctcaggctga ggaggaggag gcggcgactg 12540
aagcagttaa ggaaaaggcc caggcagagc aggaagagaa aaaacctgtc attcaacctc 12600
taaaagaaga tagcaaaaag cgcagttaca acgtcatcga gggcagcacc tttacccagt 12660
accgcagctg gtacctggcg tacaactacg gcgacccggt caagggggtg cgctcgtgga 12720
ccctgctctg cacgccggac gtcacctgcg gctccgagca gatgtattgg tcgctgccaa 12780
acatgatgca agacccggtg accttccgct ccacgcggca ggttagcaac ttcccggtgg 12840
tgggcgccga actgctgccc gtgcactcca agagttttta caacgagcag gccgtctact 12900
cccagctgat ccgccaggcc acctctctga cccacgtgtt caatcgcttt cccgagaacc 12960
agattttggc gcgcccgccg gcccccacca tcaccaccgt cagtgaaaac gttcctgccc 13020
tcacagatca cgggacgcta ccgctgcgca acagcatctc aggagtccag cgagtgacca 13080
ttactgacgc cagacgccgg acctgcccct acgtttacaa ggccttgggc atagtctcgc 13140
cgcgcgtcct ctccagtcgc actttttaaa atacatctac cctcacgctt caaaatcatg 13200
tccgtactca tctcacccag caacaacacc ggctgggggc tgcgcgcgcc cagcaagatg 13260
tttggagggg cgaggaaacg ctccgagcag cacccagtgc gcgtgcgcgg ccactaccgc 13320
gcgccctggg gagcgcacaa gcgcgggcgc gcagggcgca ccactgtgga cgacgccatt 13380
gactccgtag tggagcaggc gcgccactac acacccggcg cgccgtccgc ccccgccgtg 13440
tccaccgtgg acgaggcgat cgagagcgtg gtacagggcg cgcggcacta tgccaacctt 13500
aaaaatcgac gccgtcgcgt ggctcgccgc catcgccgga gaccccgggc caccgccgcc 13560
gcgcgccttg ctaaggctct gctcaggcgc gccaggcgaa ctggccgccg ggccgccatg 13620
agggccgcac ggcgggctgc cgccagcgcg gccgccgcgg ccccacgggc acgaaggcgc 13680
gcggccgctg ccgccgccgc cgccatttcc agcttggcct cgacgcggcg cggtaacata 13740
tactgggtgc gcgactcggt aaccggcacg cgggtacccg tgcgctttcg ccccccgcgg 13800
aattagcaca agacaacata cacactgagt ctcctgctgt tgtgtatccc agcggcgacc 13860
gtcagcagcg gcgacatgtc caagcgcaaa attaaagaag agatgctcca ggtcatcgcg 13920
ccggagatct atgggccccc gaagaaggag gaggatgatt acaagccccg caagctaaag 13980
cgggtcaaaa agaaaaagaa agatgatgac gttgacgagg cggtggagtt tgtccgccgc 14040
atggcgccca ggcgccccgt gcagtggaag ggtcggcgcg tgcagcgagt cctgcgcccc 14100
ggcaccgcgg tggtctttac gcccggcgag cgttccacgc gcactttcaa gcgggtgtac 14160
gatgaggtgt acggcgacga ggatctgttg gagcaggcca accatcgctt tggggagttt 14220
gcatatggga aacggccccg cgagagccta aaagaggacc tgctggcgct accgctggac 14280
gagggcaatc ccaccccgag tctgaagccg gtaaccctgc aacaggtgct gcctttgagc 14340
gcgcccagcg agcagaagcg agggttgaag cgcgagggcg gggacctggc acccaccgtg 14400
cagttgatgg tgcccaagcg gcagaagctg gaggacgtgc tggagaaaat gaaagtagag 14460
cccgggatcc agcccgaaat caaggtccgc cccatcaagc aggtggcgcc cggcgtggga 14520
gtccagaccg tggacgttag gattcccacg gaggagatgg aaacccaaac cgccactccc 14580
tcttcggcgg ctagcgccac caccggctcc gcttcggtag aggtgcagac ggacccctgg 14640
ctagccgccg ccgccccggc cgccccccgt tcgcgcgggc gcaagagaaa ttatccagcg 14700
gccagcgcgc tcatgcccca gtacgcactg catccatcca tcgcgcccac ccccggctac 14760
cgcgggtact cgtaccgccc gcgcagatca gccggcaccc gcggccgccg ccgccgtgcg 14820
accacaacca gccgccgccg tcgccgccgc cgccagccag tgctgacccc cgtgtctgta 14880
aggaaggtgg ctcgctcggg gagcacgctg gtggtgccca gagcgcgcta ccaccccagc 14940
attgtttaaa gccggtctct gtatggttct tgcagatatg gccctcactt gtcgcctccg 15000
cttcccggtg ccgggatacc gaggaagaac tcaccgccgc agaggcatgg cgggcagtgg 15060
tctccgcggc ggccgtcgcc atcgccggcg cgcaaagagc aggcgcatgc gcggcggtgt 15120
gctgcccttc ctaatcccgc taatcgccgc ggcgatcggt gccgtgcccg ggatcgcctc 15180
cgtggccctg caggcgtccc agaaacattg actcttgcaa ccttgcaagc ttgcattttt 15240
tggaggaaaa aataaaaagt ctagactctc acgctcgctt ggtcctgtga ctattttgta 15300
gaaaaaagat ggaagacatc aactttgcgt cgctggcccc gcgtcacggc tcgcgcccgt 15360
tcatgggaga ctggacagat atcggcacca gcaatatgag cggtggcgcc ttcagctggg 15420
gcagtctgtg gagtggcctt aaaaattttg gttccaccat taagaactat ggcaacaaag 15480
cgtggaacag cagcacgggt cagatgctga gagacaagtt gaaagagcag aacttccagg 15540
agaaggtggc acagggcctg gcctctggca tcagcggggt ggtggacata gctaaccagg 15600
ccgtgcagaa aaagataaac agtcatctgg acccccgccc tcaggtggag gaaacgcctc 15660
cagccatgga gacggtgtct cccgagggca aaggcgaaaa gcgcccgcgg cccgacaggg 15720
aagagaccct ggtgtcacac accgaggagc cgccctctta cgaggaggca gtcaaggccg 15780
gcctgcccac cactcgcccc atagctccca tggccaccgg tgtggtgggt cacaggcaac 15840
acacccccgc gacactagat ctgcccccgc cgtccgagcc gactcgccag ccaaaggcgg 15900
tgacggtgcc cgctccctcc acttccgccg ccaacagagt gcctctgcgc cgcgctgcga 15960
gcggcccccg ggcctcgcga gtcagcggca actggcagag cacactgaac agcatcgtgg 16020
gcctgggagt gaggagtgtg aagcgccgcc gttgctactg aatgagcaag ctagctaacg 16080
tgttgtatgt gtgtatgcgt cctatgtcgc cgccagagga gctgttgagc cgccggcgcc 16140
gtctgcactc cagcgaattt caagatggcg accccatcga tgatgcctca gtggtcgtac 16200
atgcacatct cgggccagga cgcttcggag tacctgagcc ccgggctggt gcagttcgcc 16260
cgcgccacag acacctactt caacatgagt aacaagttca ggaaccccac tgtggcgccc 16320
acccacgatg tgaccacgga ccggtcgcag cgcctgacgc tgcggttcat ccccgtggat 16380
cgggaggaca ccgcttactc ttacaaggcg cggttcacgc tggccgtggg cgacaaccgc 16440
gtgctggaca tggcctccac ttactttgac atccgggggg tgctggacag gggccccact 16500
ttcaagccct actcgggcac tgcctacaac tccctggccc ccaagggcgc tcccaattct 16560
tgcgagtggg aggaggaaac acaaaatgag gtacaagcca atgaagaaca actagcagaa 16620
gaagaggatg aagaaatggc tcaagaggat cagcagccta ctaaaaaaac ccatgtatat 16680
gctcaggcac ctctttctgg cgaacagatt accaaagatg gcttgcaaat aggagctgaa 16740
gttacaggag aaacatcaaa gcccattttt gcagacaaga cattccaacc agaacctcag 16800
ataggagagt ctcaatggaa tgaggccgat gctacagtag caggaggtag ggttttgaaa 16860
aagactaccc ctatgaaacc ttgctatgga tcctatgcca gacctaccaa tgccaatgga 16920
gggcagggga tacttgaggc aaatgctaaa ggggaactcg aatctaaagt tgagatgcag 16980
tttttctcta acaccacaac tcttaatgta agagacggtg aaaatggcct taaaccaaaa 17040
gtagtgctgt atagcgaaga tgtcaacctg gaatcccctg acactcatct gtcttacaag 17100
cccaaaaaag atgatgttaa tgccaaaatc atgttgggtc agcaagccat gcccaacaga 17160
cccaacctca ttggatttag agataatttc attgggctca tgtattacaa cagcactgga 17220
aacatgggag tgctggcggg tcaggcctct cagttgaatg ctgtggtgga cttgcaggat 17280
agaaacacgg aactgtcata tcagcttatg cttgattcca ttggagatag aaccagatac 17340
ttttccatgt ggaaccaggc agtggatagc tatgacccag atgttagaat cattgaaaac 17400
catggggtgg aggatgagct gcccaactac tgttttccct tgggcggtat aggaattaca 17460
gatacatacc aggccataaa agcagccaat ggtggagatg ctactacgtg gtctgctgat 17520
aacacatttg cagaccgcaa cgaaataggg gtgggaaaca acttcgccat ggagatcaac 17580
atccaggcca acctctggag aaacttcctc tatgcgaacg tgggactcta cctgccagac 17640
aagctcaagt acaaccccac caacgtggac atctctgaca accccaacac ctatgactac 17700
atgaacaagc gggtggtggc ccccggcctg gtggactgct ttgtcaatgt gggagccagg 17760
tggtccctgg actacatgga caacgtcaac cccttcaacc accaccgcaa tgcgggtctg 17820
cgctaccgct ccatgatcct gggcaacggg cgctatgtgc ccttccacat ccaggtaccc 17880
cagaagttct ttgccatcaa gaacctcctg ctcctgcccg gctcctacac ctacgagtgg 17940
aacttcagga aggatgtaaa catggtccta cagagctctc tgggcaatga ccttagggta 18000
gatggggcca gcatcaagtt tgacagcatc accctctatg ctacattttt ccccatggcc 18060
cacaacaccg cctccacgct tgaggccatg ctgagaaacg acaccaacga ccagtccttc 18120
aatgactacc tctctggggc caacatgctc tacccaatcc cagccaaggc caccaacgtg 18180
cccatctcca tcccctctcg caactgggcc gcctttagag gctgggcctt tacccgcctt 18240
aagaccaagg agaccccctc cctgggctcg ggttttgatc cctactttgt ttactcggga 18300
tccatcccct acctggatgg caccttctac ctcaaccaca ctttcaagaa gatatccatc 18360
atgtatgact cctccgtcag ctggccgggc aacgaccgct tgctcacccc caatgagttc 18420
gaggtcaagc gcgccgtgga cggcgagggc tacaacgtgg cccagtgcaa catgaccaag 18480
gactggttcc tggtgcagat gctggccaac tacaacatag gctaccaggg cttctacatc 18540
ccagagagct acaaggacag gatgtattcc ttcttcagaa atttccaacc catgagccga 18600
caggtggtgg acgagaccaa ttacaaggac tatcaggcca ttggcatcac ccaccagcac 18660
aacaactcgg gtttcgtggg ctacctggcg cccaccatgc gcgagggaca ggcctacccc 18720
gccaacttcc cctaccccct gataggcaag accgcggtcg acagcgtcac ccagaaaaag 18780
ttcctctgcg accgcaccct ctggcgcatc cccttctcta gcaactttat gtccatgggt 18840
gcgctcacgg acctgggcca aaacctgctt tatgccaact ctgcccatgc gctggacatg 18900
acttttgagg tggaccccat ggacgagccc acccttctct atattgtgtt tgaagtgttc 18960
gacgtggtca gagtgcacca gccgcaccgc ggtgtcatcg agaccgtgta cctgcgtacg 19020
cccttctcag ccggcaacgc caccacctaa ggagacagcg ccgccgcctg catgactggt 19080
tccaccgagc aagagctcag ggccatcgcc agagacctgg gatgcggacc ctattttttg 19140
ggcacctatg acaaacgctt cccgggtttt atctcccgag acaagctcgc ctgcgccatc 19200
gtcaacacgg ccgcgcgcga gaccgggggc gtgcactggc tggcctttgg ctgggacccg 19260
cgctctaaaa cttgctacct ctttgacccc tttggcttct ccgatcagcg cctcaggcag 19320
atttatgagt ttgagtacga ggggctgttg cgccgcagcg cgcttgcctc ctcgcccgac 19380
cgctgcatca cccttgagaa gtccaccgag accgtgcagg ggccccactc ggccgcctgc 19440
ggtctcttct gttgcatgtt tttgcacgcc tttgtgcact ggcctcagag tcccatggat 19500
cgcaacccca ccatgaactt gctaaaggga gtgcccaacg ccatgctcca gagcccccag 19560
gtcctgccca ccctgcgccg caaccaggaa cagctctacc gcttcctgga gcgccactcc 19620
ccctacttcc gcagccacag cgcgcgcatc cggggggcca cctctttttg ccacttgcaa 19680
gaaaacatgc aagacggaaa atgatgtaca gcatgctttt aataaatgta aagactgtgc 19740
actttattta tacacgggct ctttctggtt atttattcaa caccgccgtc gccatctaga 19800
aatcgaaagg gttctgccgc gcgtcgccgt gcgccacggg cagagacacg ttgcgatact 19860
ggaagcggct cgcccacttg aactcgggca ccaccatgcg gggcagtggt tcctcgggga 19920
aattctcgct ccacagggtg cgggtcagct gcagcgcgct caggaggtcg ggagccgaga 19980
tcttgaagtc gcagttgggg ccggaaccct gcgcgcgcga gttgcggtac acggggttgc 20040
agcactggaa caccagcagg gccggattat tcacgctggc cagcaggctc tcgtcgctaa 20100
tcatgtcgct gtccagatcc tccgcgttgc tcagggcgaa tggggtcatc ttgcagacct 20160
gcctgcccag gaaaggcggg agcccaggct tgccgttaca gtcgcagcgc aggggcatta 20220
gcaggtgccc acggcccgac tgcgcctgcg ggtacaacgc gcgcatgaag gcttcgatct 20280
gcctaaaagc cacctgggtc ttggctccct ccgaaaagaa catcccacag gacttgctgg 20340
agaactggtt cgcgggacag ctggcatcgt gcaggcagca gcgcgcgtca gtgttggcga 20400
tctgcaccac gttgcgaccc caccggtttt tcactatctt ggccttggaa gcctgctcct 20460
ttagcgcgcg ctggccgttc tcgctggtca catccatctc tatcacctgt tccttgttga 20520
tcatgtttgt cccgtgcaga cactttaggt cgccctccgt ctgggtgcag cggtgctccc 20580
acagcgcgca accggtgggc tcccaatttt tgtgggtcac ccccgcgtag gcctgcaggt 20640
aggcctgcag gaagcgcccc atcatggtca taaaggtctt ctggctcgta aaggtcagct 20700
gcaggccgcg atgctcttcg ttcagccagg tcttgcagat ggcggccagc gcctcggtct 20760
gctcgggcag catcttaaaa tttgtcttca ggtcgttatc cacgtggtac ttgtccatca 20820
tggcacgcgc cgcctccatg cccttctccc aggcggacac catgggcagg cttagggggt 20880
ttatcacttc cagcggcgag gacaccgtac tttcgatttc ttcttcctcc ccctcttccc 20940
ggcgcgcgcc cccgctgttg cgcgctctta ccgcctgcac caaggggtcg tcttcaggca 21000
agcgccgcac cgagcgcttg ccgcccttga cctgcttgat cagtaccggc gggttgctga 21060
agcccaccat ggtcagcgcc gcctgctctt cttcgtcttc gctgtctacc actatttctg 21120
gggaggggct tctccgctct gcggcaaagg cggcggatcg cttctttttt ttcttgggag 21180
ccgccgcgat ggagtccgcc acggcgaccg aggtcgaggg cgtggggctg ggggtgcgcg 21240
gcaccagggc ctcgtcgccc tcggactctt cctctgactc caggcggcgg cggagtcgct 21300
tctttggggg cgcgcgcgtt agcggcggcg gagacgggga cggggacggg gacgggacgc 21360
cctccacagg gggcggtctt cgcgcagacc cgcggccgcg ctcgggggtc ttctcgcgct 21420
ggtcttggtc ccgactggcc attgtatcct cctcctccta ggcagagaga cataaggagt 21480
ctatcatgca agtcgagaag gaggagagct taaccacccc ctctgagacc gccgtcgccg 21540
tcgcccccgc taccgccgac gcgcccgcca caccgagcga cacccccgcg gacccccccg 21600
ccgacgcacc cctgttcgag gaagcggccg tggagcagga cccgggcttt gtctcggcag 21660
aggaggattt gcaagaggag gaggataagg aggagaagcc ctcagtgcca aaagatcata 21720
aagagcaaga cgagcacgac gcagacgcac accagggtga agtcgggcgg ggggacggag 21780
ggcatggcgg cgccgactac ctagacgaag gaaacgacgt gctcttgaag cacctgcatc 21840
gtcagtgcgc cattgtctgc gacgctctgc aggagcgcag cgaggtgccc ctcagcgtgg 21900
cggaggtcag ccgcgcctac gagctcagcc tcttttcccc ccgggtgccc ccccgccgcc 21960
gcgaaaacgg cacatgcgag cccaacccgc gcctcaactt ctaccccgcc tttgtggtgc 22020
ccgaggtcct ggccacctat cacatcttct ttcaaaattg caagatcccc atctcgtgcc 22080
gcgccaaccg tagccgcgcc gataagatgc tggccctgcg ccagggcgac cacatacctg 22140
atatcgccgc tttggaagat gtgccaaaga tcttcgaggg tctgggtcgc aacgagaagc 22200
gggcagcaaa ctctctgcaa caggaaaaca gcgaaaatga gagtcacact ggagcgctgg 22260
tggagctgga gggcgacaac gcccgcctgg cggtgctcaa gcgcagcatc gaggtcaccc 22320
actttgccta ccccgcgctc aacctgcccc ccaaagtcat gaacgcggtc atggacgggc 22380
taatcatgcg ccgcggccgg ccccttgctc cagatgcaaa cttgcatgag gagaccgagg 22440
acggtcagcc cgtggtcagc gacgagcagc tgacgcgctg gctggaaacc gcggaccccg 22500
ccgaactgga ggagcggcgc aagatgatga tggccgcggt gctggtcacc gtagagctgg 22560
agtgtctgca gcgcttcttc ggcgaccccg agatgcagag aaaggtcgag gagaccctac 22620
actacacctt ccgccagggc tacgtgcgcc aggcttgcaa gatctccaac gtggagctca 22680
gcaacctggt gtcctacctg ggcatcttgc atgaaaaccg ccttgggcag agcgtgctac 22740
actccaccct gcgcggggag gcgcgccgcg actacgtgcg cgactgcgtt tacctcttcc 22800
tctgctacac ctggcagacg gccatggggg tctggcagca gtgcctggag gagcgcaacc 22860
tcaaggagct ggagaagctc ctgcagcgcg cgctcaaaga cctctggacg ggctacaacg 22920
agcgctcggt ggccgccgcg ctggccgacc tcatcttccc cgagcgcctg ctcaaaactc 22980
tccagcaggg gctgcccgac ttcaccagcc aaagcatgtt gcaaaatttt aggaacttta 23040
tcctggagcg ttctggcatc ctacccgcca cctgctgcgc cctgcccagc gactttgtcc 23100
ccctcgtgta ccgcgagtgc cccccgccgc tgtggggcca ctgctacctg ttccaactgg 23160
ccaactacct gtcctaccac gcggacctca tggaagactc cagcggcgag gggctcatgg 23220
agtgccactg ccgctgcaac ctctgcacgc cccaccgctc cctggtctgc aacacccaac 23280
tgctcagcga gagtcagatt atcggtacct tcgagctaca gggtccgtcc tcctcagacg 23340
agaagtccgc ggctccgggg ctaaaactca ctccggggct gtggacttcc gcctacctgc 23400
gcaaatttgt acctgaagac taccacgccc acgagatcag gttttacgag gaccaatccc 23460
gcccgcccaa ggcggagctg accgcctgcg tcatcaccca gggcgagatc ctaggccaat 23520
tgcaagccat ccaaaaagcc cgccaagagt ttttgctgag aaagggtcgg ggggtgtatc 23580
tggaccccca gtcgggtgag gagctcaacc cggttccccc gctgccgccg ccgcgggacc 23640
ttgcttccca ggataagcat cgccatggct cccagaaaga agcagcagcg gccgccactg 23700
ccgccacccc acacgctgga ggaagaggag gaatactggg acagtcaggc agaggaggtt 23760
tcggacgagg aggagccgga gacggagatg gaagagtggg aggaggacag cttagacgag 23820
gaggcttccg aagccgaaga ggcaggcgca acaccgtcac cctcggccgc agccccctcg 23880
caggcgcccc cgaagtccgc tcccagcatc agcagcaaca gcagcgctat aacctccgct 23940
cctccaccgc cgcgacccac ggccgaccgc agacccaacc gtagatggga caccaccgga 24000
accggggccg gtaagtcctc cgggaaaggc aagcaagcgc agcgccaagg ctaccgctcg 24060
tggcgcgctc acaagaacgc catagtcgct tgcttgcaag actgcggggg gaacatctcc 24120
ttcgcccgcc gcttcctgct cttccaccac ggtgtggcct tcccccgtaa cgtcctgcat 24180
tactaccgtc atctctacag cccctactgc ggcggcagtg agccagaggc ggccggcggc 24240
agcggcgccc gtttcggtgc ctaggaagac ccagggcaag acttcagcca agaaactcgc 24300
ggcggccgcg gcgaacgcgg tcgcgggggc cctgcgcctg acggtgaacg aacccctgtc 24360
gacccgcgaa ctgaggaacc gaatcttccc cactctctat gccatcttcc agcagagcag 24420
agggcaggat caggaactga aagtaaaaaa caggtctctg cgctccctca cccgcagctg 24480
tctgtatcac aagagcgaag accagcttcg gcgcacgctg gaggacgctg aggcactctt 24540
cagcaaatac tgcgcgctca ctcttaagga ctagctccgc gcccttctcg aatttaggcg 24600
ggaacgccta cgtcatcgca gcgccgccgt catgagcaag gacattccca cgccatacat 24660
gtggagctat cagccgcaga tgggactcgc ggcgggcgcc tcccaggatt actccacccg 24720
catgaactgg ctcagtgccg gcccacacat gatctcacag gttaatgaca tccgcaccca 24780
tcgaaaccaa atattggtgg agcaggcggc aattaccacc acgccccgca ataatcccaa 24840
ccccagggag tggcccgcgt ccctggtgta tcaggaaatt cccggcccca ccaccgtact 24900
acttccgcgt gattcccagg ccgaagtcca aatgactaac tcaggggcac agctcgcggg 24960
cggctgtcgt cacagggtgc ggcctcctcg ccagggtata actcacctga agatccgagg 25020
cagaggtatt cagctcaacg acgagtcggt gagctcctcg ctcggtctca gacctgacgg 25080
gaccttccag atagccggag ccggccgatc ttccttcacg ccccgccagg cgtacctgac 25140
tctgcagagc tcgtcctcgg cgccgcgctc gggcggcatc gggactctcc agttcgtgca 25200
ggagtttgtg ccctcggtct acttcaaccc cttctcgggc tctcccggtc gctacccgga 25260
ccagttcatc ccgaactttg acgccgcgag ggactcggtg gacggctacg actgaatgtc 25320
gggtggaccc ggtgcagagc aacttcgcct gaagcacctt gaccactgcc gccgccctca 25380
gtgctttgcc cgctgtcaga ccggtgagtt ccagtacttt tccctgcccg actcgcaccc 25440
ggacggcccg gcgcacgggg tgcgcttttt catcccgagt caggtccgct ctaccctaat 25500
cagggagttc accgcccgtc ccctactggc ggagttggaa aaggggcctt ctatcctaac 25560
cattgcctgc atctgctcta accctggatt acaccaagat ctttgctgtc atttgtgtgc 25620
tgagtataat aaaggctgag atcagaatct actcgggctc ctgtcgccat cctgtcaacg 25680
ccaccgtcca agcccggccc gatcagcccg aggtgaacct cacctgcggt ctgcaccggc 25740
gcctgaggaa atacctagct tggtactaca acagcactcc ctttgtggtt tacaacagct 25800
ttgaccagga cggggtctca ctgagggata acctctcgaa cctgagctac tccatcagga 25860
agaacaacac cctcgagcta cttcctcctt acctgcccgg gacttaccag tgtgtcaccg 25920
gtccctgcac ccacacccac ctgttgatcg taaacgactc tcttccgaga acagacctca 25980
ataactcctc tccgcagttc cccagaacag gaggtgagct caggaaaccc cgggtaaaga 26040
agggtggaca agagttaaca cttgtggggt ttctggtgta tgtgacgctg gtggtggctc 26100
ttttgattaa ggcttttcct tccatgtctg aactatccct cttcttttat gaacaactcg 26160
actagtgcta acgggaccct acccaacgaa tcgggattga atatcggtaa ccaggttgca 26220
gtttcacttt tgattacctt catagtcctc ttcctgctag tgctgtcgct tctgtgcctg 26280
cggatcgggg gctgctgcat ccacgtttat atctggtgct ggctgtttag aaggttcgga 26340
gaccaccgca ggtagaataa acaaacctag acctagaaat ggacggtctc tgcagcgagc 26400
aacgcatact agagaggcgc cggcaaaaag cagagctcga gcgtcttaaa caagagctcc 26460
aagacgccgt ggccatacac cagtgcaaaa aagggctctt ctgtctggta aaacaggcca 26520
cgctcaccta tgaaaaaaca ggtgacaccc accgcctagg atacaagctg cccacacagc 26580
gccaaaagtt tgcccttatg ataggtgaac aacccatcac cgtgacccag cactccgtgg 26640
agacagaagg ctgcattcat gctccctgca ggggcgctga ctgcctctac accttgatca 26700
aaaccctttg cggtctcaga gaccttatcc ctttcaattg atcataactg taatcaataa 26760
aaaatcactt acttgaaatc tgatagcaag cctctgtcca attttttcag caacacttcc 26820
ttcccctctt cccaactctg gtactctagg cgcctcctag ctgcaaactt cctccacagt 26880
ctgaagggaa tgtcagattc ctcctcctcc tgtccctccg cacccacgat cttcatgttg 26940
ttgcagatga agcgcaccaa aacgtctgac gagagcttca accccgtgta cccctatgac 27000
acggaaaacg gtcctccctc cgtccctttc ctcacccctc ccttcgtgtc tcccgatgga 27060
ttccaagaga gcccccccgg ggtcctgtct ctgaacctgg ccgagcccct ggtcacttcc 27120
cacggcatgc tcgccctgaa aatgggaagt ggcctctccc tggacgacgc cggcaacctc 27180
acctctcaag atgtcaccac cactacccct cccctgaaaa aaaccaagac caacctcagc 27240
ctagaaacct cagcccccct gactgtgagc acctcaggcg ccctcaccct agcagccgcc 27300
gttcccctgg cggtggccgg cacctccctc accatgcaat cagaggcccc cctgacagtc 27360
caagatgcaa aactcaccct ggccaccaag ggccccctga ccgtgtctga aggcaaactg 27420
gccttgcaga cctcggcccc gctgacggcc gctgacagca gcgccctcac cgttagcgcc 27480
acaccaccca tcagtgtaag cagtggaagt ttgggcttag acatggaaga ccccatgtat 27540
actcatgatg gaaaactggg aataagaatt ggaggcccac tgagagtagt agacagcctg 27600
cacacactga ctgtagttac cggaaatgga atagctgtag ataacaatgc cctccaaact 27660
agagttacgg gcgccctggg ttatgacaca tcaggaaacc tacaactgag agccgcgggg 27720
ggtatgcgaa ttgatgcaaa tggccaactt atccttgatg tggcataccc atttgatgct 27780
caaaacaatc tcagccttag acttggtcag ggacccctgt atgtaaacac agaccacaac 27840
ctagatttga attgcaacag aggtctgacc acaactacca ccaacaacac aaaaaaactt 27900
gaaactaaaa ttggctcagg cttagactat gataccaatg gtgctgtcat tattaaactt 27960
ggtactggtg taagctttga cagcacaggc gccctaactg tgggaaacac tggcgatgat 28020
aaactgactc tgtggacaac cccagaccca tctccaaatt gcagaattca cgcagacaaa 28080
gactgcaagt ttactctagt cctaactaag tgtggaagtc aaatcctggc ttctgtcgcc 28140
gccctagcgg tgtcaggaaa tctggcttca ataacaggca ccgttgccag cgttaccatc 28200
tttctcagat ttgatcagaa tggagtgctt atggaaaact cctccctaga caagcagtac 28260
tggaacttca gaaatggtaa ctcaaccaat gccaccccct acaccaatgc agttgggttc 28320
atgccaaacc tcgcagcata ccccaagaca cagagccaga ctgctaaaaa caacattgta 28380
agtcaggttt acttgaatgg ggacaaatcc aaacccatga cccttaccat taccctcaat 28440
ggaactaatg aatccagtga aactagccag gtgagtcact actccatgtc atttacatgg 28500
gcttgggaga gtgggcaata tgccaccgaa acctttgcca ccaattcctt taccttctct 28560
tacattgctg aacaataaag aaagcacaga gatgcttgtt tttgatttca aaattgtgtg 28620
cttttattta ttttcaagct tacagtattt ccagtagtca ttcgaataga gcttaatgaa 28680
actgcatgag aacccttcca catagcttaa attatcacca gtgcaaatgg agaaaaatca 28740
acataccttt ttatccagat atcacagaac cctagtattc aacctgccac ctccctccca 28800
acacacagag tacacagtcc tttctccccg gctggcctta aaaagcatca tatcatgggt 28860
aacagacata ttcttaggtg ttatattcca cacggtttcc tgtcgagcca aacgctcatc 28920
agtgatatta ataaactccc cgggcagctc acttaagttc atgtcgctgt ccagctgctg 28980
agccacaggc tgctgtccaa cttgcggttg cttaacgggc ggcgaaggag aagtccacgc 29040
ctacatgggg gtagagtcat aatcgtgcat caggataggg cggtggtgct gcagcagcgc 29100
gcgaataaac tgctgccgcc gccgctccgt cctgcaggaa tacaacatgg cagtggtctc 29160
ctcagcgatg attcgcaccg cccgcagcat aaggcgcctt gtcctccggg cacagcagcg 29220
caccctgatc tcacttaaat cagcacagta actgcagcac agcaccacaa tattgttcaa 29280
aatcccacag tgcaaggcgc tgtatccaaa gctcatggcg gggaccacag aacccacgtg 29340
gccatcatac cacaagcgca ggtagattaa gtggcgaccc ctcataaaca cgctggacat 29400
aaacattacc tcttttggca tgttgtaatt caccacctcc cggtaccata taaacctctg 29460
attaaacatg gcgccatcca ccaccatcct aaaccagctg gccaaaacct gcccgccggc 29520
tatacactgc agggaaccgg gactggaaca atgacagtgg agagcccagg actcgtaacc 29580
atggatcatc atgctcgtca tgatatcaat gttggcacaa cacaggcaca cgtgcataca 29640
cttcctcagg attacaagct cctcccgcgt tagaaccata tcccagggaa caacccattc 29700
ctgaatcagc gtaaatccca cactgcaggg aagacctcgc acgtaactca cgttgtgcat 29760
tgtcaaagtg ttacattcgg gcagcagcgg atgatcctcc agtatggtag cgcgggtttc 29820
tgtctcaaaa ggaggtagac gatccctact gtacggagtg cgccgagaca accgagatcg 29880
tgttggtcgt agtgtcatgc caaatggaac gccggacgta gtcatatttc ctccagcaga 29940
accaagtgcg cgcgtggcag ctatccttgc gtcttctgtc tcgccgcctg ccccgctcgg 30000
tgtagtagtt gtaatacagc cactccctca gaccgtcaag gcgctccctg gcgtccggat 30060
ctataacaac accatcctgc agcgccgccc tgatgacatc caccaccgta gagtatgcca 30120
agcccagcca ggaaatgcac tcactttgac agcgagagat aggaggagcg ggaagagatg 30180
gaagaaccat gatagtaaaa gaacttttat tccaatcgat cctctacaat gtcaaagtgt 30240
agatctatca gatggcactg gtctcctccg ctgagtcgat caaaaataac agctaaacca 30300
caaacaacac gattggtcaa atgctgcaca agggcttgca gcataaaatc gcctcgaaag 30360
tccaccgcaa gcataacatc aaagccaccg cccctatcat gatctatgat aaaaacccca 30420
cagctatcca ccagacccat atagttttca tctctccatc gtgaaaaaat atttacaagc 30480
tcctccttta aatcacctcc aaccaattca aaaagttgag ccagaccgcc ctccaccttc 30540
attttcagca tgcgcatcat gattgcaaaa attcaggctc ctcagacacc tgtataagat 30600
tgagaagcgg aacattaaca tcaatgtttc gctcgcgaag atcgcgcctc agtgcaagca 30660
tgatataatc ccacaggtcg gagcggatca gcgaggacat ctccccgcca ggaaccaact 30720
caacggagcc tatgctgatt ataatacgca tattcggggc tatgctaacc agcacggccc 30780
ccaaataggc gtactgcata ggcggcgaca aaaagtgaac agtttgggtt aaaaaatcag 30840
gcaaacactc gcgcaaaaaa gcaagaacat cataaccatg ctcatgcaaa tagatgcaag 30900
taagctcagg aacgaccaca gaaaaatgca caatttttct ctcaaacatg actgcgagcc 30960
ctgcaaaaaa taaaaaagaa acattacaca agagtagcct gtcttacaat gggatagact 31020
actctaacca acataagacg ggccacgaca tcgcccgcgt ggccataaaa aaaattatcc 31080
gtgtgattaa aaagaagcac agatagctgg ccagtcatat ccggagtcat cacgtgcgaa 31140
cccgtgtaga cccccgggtt ggacacatcg gccaaagaaa gaaagcggcc aatgtatccc 31200
ggaggaatga taacactaag acgaagatac aacagaataa ccccatgggg gggaataaca 31260
aagttagtag gtgaataaaa acgataaaca cccgaaactc cctcctgcgt aggcaaaata 31320
gcgccctccc cttccaaaac aacatacagc gcttccacag cagccatgac aaaagactca 31380
aaacactcaa aagactcagt cttaccagga aaataaaagc actctcacag caccagcact 31440
aatcagagtg tgaagagggc caagtgccga acgagtatat ataggaatta aaaatgacgt 31500
aaatgtgtaa aggtcaaaaa acgcccagaa aaatacacag accaacgccc gaaacgaaaa 31560
cccgcgaaaa aatacccaga agttcctcaa caaccgccac ttccgctttc ccacgatacg 31620
tcacttcctc gaaaatagca aactacattt cccacatgta caaaaccgaa accactcccc 31680
ttgtcaccgc ccacaactta catcttaatt aacaaacgtc aaagcctacg tcagccgccc 31740
cgcctcgccc cgcccacctc attatcatat tggccacaat ccaaaataag gtatattatt 31800
gatgatgatt taaatattat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac 31860
cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 31920
cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 31980
aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 32040
gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 32100
tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 32160
agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 32220
ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 32280
taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 32340
gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 32400
gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 32460
ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 32520
ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 32580
gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 32640
caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 32700
taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 32760
aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 32820
tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 32880
tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 32940
gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 33000
gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 33060
aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 33120
gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 33180
ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 33240
tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 33300
atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 33360
ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 33420
ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 33480
ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 33540
atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 33600
gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 33660
tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 33720
ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 33780
acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc 33840
tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattgattta aat 33893
<210> 10
<211> 33742
<212> DNA
<213> 人工序列
<220>
<223> pBZ1_BZ28F.5IXP
<400> 10
catcatcaat aatatacctt attttggatt gtggccaata tgataatgag gtgggcgggg 60
cgaggcgggg cgggtgacgt aggacgtgcg agtagggttg ggaggtgtgg cggaagtgtg 120
gcatttgtaa gtgggaggag cttacatgca agcttccgtc gcggaaaatg tgacgttttt 180
tatgagcgcc gcctacctcc ggaagtgcca attttcgcgc gctttttacc ggatatcgta 240
gtaattttgg gcgggaccat gtaagatttg gccattttcg cgcgaaaagt gaaacgggga 300
agtgaaaact gaataatagg gcgttagtca tagcgcgtaa tatttaccga gggccgaggg 360
actttgaccg attacgtgga ggactcgccc aggtgttttt tacgtgaatt tccgcgttcc 420
gggtcaaagt ctccgttttt attgtcaccg tcatttgacg cggagggtat ttaaacccgc 480
tgcgctccta gcgatcgcgg tactgaaatg tgtgggcgtg gcttaagggt gggaaagaat 540
atataaggtg ggggtcttat gtagttttgt atctgttttg cagcagccgc cgccgccatg 600
agcgacaccg gcaacagctt tgatggaagc atctttagcc cctatctgac agtgcgcatg 660
cctcactggg ccggagtgcg tcagaatgtg atgggttcca acgtggatgg acgtcccgtt 720
ctgccttcaa attcgtctac gatggcctac gcgaccgtgg gaggaactcc gctggacgcc 780
gcgacctccg ccgccgcctc cgccgccgcc gcgaccgcgc gcagcatggc tacggacctt 840
tacagctctt tggtggcgag cagcgcggcc tctcgcgcgt ctgctcggga tgaaaaactg 900
actgctctgc tgcttaaact ggaagacttg acccgggagc tgggtcaact gacccagcag 960
gtctccagct tgcgtgagag cagccttgcc tccccctaat ggcccataat ataaataaaa 1020
gccagtctgt ttggattaag caagtgtatg ttctttattt aactctccgc gcgcggtaag 1080
cccgggacca gcggtctcgg tcgtttaggg tgcggtggat tctttccaac acgtggtaca 1140
ggtggctctg gatgtttaga tacatgggca tgagtccatc cctggggtgg aggtagcacc 1200
actgcagagc ttcgtgctcg ggggtggtgt tgtatatgat ccagtcgtag caggagcgct 1260
gggcgtggtg ctgaaaaatg tccttaagca agaggcttat agctaggggg aggcccttgg 1320
tgtaagtgtt tacaaatctg cttagctggg aggggtgcat ccggggggat atgatgtgca 1380
tcttggactg gatttttagg ttggctatgt tcccacccag atcccttctg ggattcatgt 1440
tgtgcaggac caccagcacg gtatatccag tgcacttggg aaatttatcg tggagcttag 1500
acgggaatgc atggaagaac ttggagacgc ccttgtggcc tcccagattt tccatacatt 1560
cgtccatgat gatggcaatg ggcccgtggg aagctgcctg agcaaaaacg tttctggcat 1620
cgctcacatc gtagttatgt tccagggtga ggtcatcata ggacatcttt acaaatcggg 1680
ggcggagggt cccggactgg gggatgatgg taccctcggg ccccggggcg tagttcccct 1740
cacagatctg catctcccag gctttcattt cagagggagg gatcatatcc acctgcgggg 1800
cgatgaaaaa gacagtttct ggcgcagggg agattaactg ggatgagagc aggtttctga 1860
gcagctgtga ctttccacag ccggtgggcc catatatcac gcctatcacc ggctgcagct 1920
ggtagttaag agagctgcag ctgccgtcct cccggagcag gggggccacc tcgttgagca 1980
tatccctgac gtggatgttc tccctgacca gttccgccag aaggcgctcg ccgcccagcg 2040
aaagcagctc ttgcaaggaa gcaaaatttt tcagcggttt caggccatcg gccgtgggca 2100
tgtttttcag cgtctgggtc agcagctcca gcctgtccca gagctcggtg atgtgctcta 2160
cggcatctcg atccagcaga tctcctcgtt tcgcgggttg gggcggcttt cgctgtaggg 2220
caccagccga tgggcgtcca gcggggccag agtcatgtcc ttccatgggc gcagagtcct 2280
cgtcagggtg gtctgggtca cggtgaaggg gtgcgctccg ggttgggcac tggccagggt 2340
gcgcttgagg ctggttctgc tggtgctgaa tcgctgccgc tcttcgccct gcgcgtcggc 2400
caggtagcat ttgaccatgg tctcgtagtc gagaccctcg gcggcgtgcc ccttggcgcg 2460
gagctttccc ttggaggtgg cgccgcacga ggggcactgc aggctcttca gggcgtagag 2520
cttgggagcg agaaacacgg actctgggga gtaggcgtcc gcgccgcagg ccgcgcagac 2580
cgtctcgcat tccaccagcc aagtgagttc cgggcggtca gggtcaaaaa ccaggttgcc 2640
cccatgcttt ttgatgcgtt tcttacctcg gctctccatg aggcggtgtc ccttctcggt 2700
gacgaagagg ctgtccgtgt ccccgtagac cgacttcagg ggcctgtctt ccagcggagt 2760
gcctctgtcc tcctcgtaga gaaactctga ccactctgag acgaaggccc gtgtccaggc 2820
caggacgaag gaggccacgt gggaggggta gcggtcgttg tccactagcg ggtccacctt 2880
ctccagggtg tgcagacaca tgtccccctc ctccgcgtcc agaaaagtga ttggcttata 2940
ggtgtaggac acgtgaccgg gggttcccga cgggggggta taaaaggggg tgggcgccct 3000
ttcatcttca ctctcttccg catcgctgtc tgcgagagcc agctgctggg gtaagtattc 3060
cctttcaaag gcgggcatga cctcagcgct caggttgtca gtttctaaaa atgaggagga 3120
tttgatgttc acctgtccgg aagtgatacc tttgagggta cctgggtcca tctggtcaga 3180
aaacactatt tttttgttgt caagcttggt ggcgaacgac ccgtagaggg cgttggagag 3240
cagcttggca atggagcgca gggtctggtt tttgtcgcgg tcggctcgct ccttggccgc 3300
gatgttgagt tgcacgtatt cgcgggccac gcacttccac tcggggaaga cggtggtgcg 3360
ctcgtctggg atcaggcgca ccctccagcc gcggttgtgc agggtgacca tgtcgacgct 3420
ggtggcgacc tcaccgcgca gacgctcgtt ggtccagcag aggcggccgc ctttgcgcga 3480
gcagaagggg ggtagggggt ccagctggtc ctcgtttggg gggtccgcgt cgatggtaaa 3540
gaccccgggg agcaggcgcg ggtcaaagta gtcgatcttg caagcttgca tgtccagagc 3600
ccgctgccat tcgcgggcgg cgagcgcgcg ctcgtagggg ttgaggggcg ggccccaggg 3660
catggggtgg gtgagcgcgg aggcgtacat gccgcagatg tcatacacgt acaggggttc 3720
cctgaggata ccgaggtagg tggggtagca gcgccccccg cggatgctgg cgcgcacgta 3780
gtcatagagc tcgtgggagg gggccagcat gttgagccca aggttggtgc gctgggggcg 3840
ctcggcgcgg aagacgatct gtctgaagat ggcatgggag ttggaggaga tggtgggtcg 3900
ctggaagacg ttgaagcttg cttcttgcaa gcccacggag tccctgacga aggaggcgta 3960
ggactcgcgc agcttgtgca ccagctcggc ggtgacctgg acgtcgagcg cgcagtagtc 4020
gagggtctcg cggatgatgt catacttatc ctcccccttc tttttccaca gctcgcggtt 4080
gaggacgaac tcttcgcggt ctttccagta ctcttggagg ggaaacccgt ccgtgtccga 4140
acggtaagag cctagcatgt agaactggtt gacggcctgg taggggcagc agcccttctc 4200
cacgggcagc gcgtaggcct gcgccgcctt gcggagggag gtgtgggtaa gggcgaaagt 4260
gtccctgacc atgactttga ggtattgatg tctgaagtct gtgtcatcgc agccgccctg 4320
ttcccacagg gtgtagtccg tgcgcttttt ggagcgcggg ttgggcaggg agaaggtgag 4380
gtcattgaag aggatcttcc ccgctcgagg catgaagttt ctggtgatgc gaaagggccc 4440
tgggaccgag gagcggttgt tgatgacctg ggcggccagg acgatctcgt caaagccgtt 4500
tatgttgtgg cccacgatgt agagctccag gaagcggggc tggcccttga tggaggggag 4560
ctttttaagt tcctcgtagg taagctcctc gggcgattcc aggccgtgct cctccagggc 4620
ccagtcttgc aagtgagggt tggccgccag gaaggatcgc cagaggtcgc gggccatgag 4680
ggtctgcagg cggtcgcgga aggttctgaa ctgccgccct acggccatct tttcgggggt 4740
gatgcagtag aaggtgaggg ggtctttctc ccaggggtcc catctgagct cttgggcgag 4800
gtcgcgcgcg gcggcgacca gagcctcgtc gccccccagt ttcatgacca gcatgaaggg 4860
cacgagctgc ttgccaaagg ctcccatcca agtgtaggtc tctacatcgt aggtgacaaa 4920
gaggcgctcc gtgcgaggat gagagccgat cgggaagaac tggatctccc gccaccagtt 4980
ggaggattgg ctgttgatgt ggtgaaagta gaagtcccgt ctgcgggccg agcactcgtg 5040
ctggcttttg taaaagcgac cgcagtactg gcagcgctgc acgggttgta tatcttgcac 5100
gaggtgaacc tggcgacctc tgacgaggaa gcgcagcggg aatctaagtc ccccgcctgg 5160
ggtcccgtgt ggctggtggt cttctacttt ggttgtctgg ccgccagcat ctgtctcctg 5220
gagggcgatg gtggagcaga ccaccacgcc gcgagagccg caggtccaga tctcggcgct 5280
cggcgggcgg agtttgatga cgacatcgcg cacattggag ctgtccatgg tctccagctc 5340
ccgcggcggc aggtcagccg ggagttcctg gaggttcacc tcgcagagac gggtcaaggc 5400
gcggacagtg ttgagatggt atctgatttc aaggggcgtg ttggaggcgg agtcgatggc 5460
ttgcagaagg ccgcagcccc ggggggccac gatggttccc cgcggggcgc gaggggaggc 5520
ggaagctggg ggtgtgttca gaagcggtga cgcgggcggg cccccggagg tagggggggt 5580
tccggcccca caggcatggg cggcaggggc acgtcttcgc cgcgcgcggg caggggctgg 5640
tgctggctcc ggagagcgct tgcgtgcgcg acgacgcgac ggttggtgtc ctgtatctgg 5700
cgcctctgag tgaagaccac gggtcccgtg accttgaacc tgaaagagag ttcgacagaa 5760
tcaatctcgg catcgttgac agcggcctgg cgcaggatct cctgcacgtc gcccgagttg 5820
tcctggtagg cgatctctgc catgaactgc tcgatctctt cctcctggag atctcctcgt 5880
ccggcgcgct ccacggtggc cgccaggtcg ttggagatgc gacccatgag ctgcgagaag 5940
gcgttgaggc cgccctcgtt ccagacccgg ctgtagacca cgcccccctc ggcgtcgcgg 6000
gcgcgcatga ccacctgggc caggttgagc tccacgtgtc gcgtgaagac ggcgtagttg 6060
cgcaggcgct ggaaaaggta gttcagggtg gtggcggtgt gttcggcgac gaagaagtac 6120
atgacccagc gccgcaacgt ggattcattg atgtccccca aggcctccag gcgctccatg 6180
gcctcgtaga agtccacggc gaagttgaaa aactgggagt tgcgagcgga cacggtcaac 6240
tcctcctcca gaagacggat gagctcggcg acagtgtcgc gcacctcgcg ctcgaaggcc 6300
acggggggcg cttcttcctc ttccacctct tcttccatga ttgcttcttc ttcctcagcc 6360
gggacgggag ggggcggcgg cgggggaggg gcgcggcggc ggcggcggcg caccggcagg 6420
cggtcgatga agcgctcgat catctccccc cgcatgcggc gcatggtctc ggtgacggcg 6480
cggccgttct cccgggggcg cagctcaaag acgccgcctc tcatctcgcc gcggggcggg 6540
cggccgtgag gtagcgagac ggcgctgact atgcatctta acaattgctg tgtaggtacg 6600
ccgccaaggg acctgattga gtccagatcc accggatccg aaaacctttg gaggaaagcg 6660
tctatccagt cgcagtcgca aggtaggctg agcaccgtgg cgggcggggg cgggtcggga 6720
gagttcctgg cggagatgct gctgatgatg taattaaagt aggcggtctt gagaaggcgg 6780
atggtggaca ggagcaccat gtctttgggt ccggcctgtt ggatgcggag gcggtcggcc 6840
atgccccagg cctcgttctg acaccggcgc aggtctttgt agtagtcttg catgagtctt 6900
tccaccggca cctcttctcc ttcctcttct ccatctcgcc ggtggtttct cgcgccgccc 6960
atgcgcgtga ccccaaagcc cctgagcggc tgcagcaggg ccaggtcggc gaccacgcgc 7020
tcggccaaga tggcctgctg tacctgagtg agggtcctct cgaagtcatc catgtccacg 7080
aagcggtggt aggcgcccgt gttgatggtg taggtgcagt tggccatgac ggaccagttg 7140
acggtctggt gtcccggctg cgagagctcc gtgtaccgca ggcgcgagaa ggcgcgggaa 7200
tcgaacacgt agtcgttgca agtccgcacc agatactggt agcccaccag gaagtgcggc 7260
ggaggttggc gatagagggg ccagcgctgg gtggcggggg cgccgggcgc caggtcttcc 7320
agcatgaggc ggtggtatcc gtagatgtac ctggacatcc aggtgatgcc ggcggcggtg 7380
gtggtggcgc gcgcgtagtc gcggacccgg ttccagatgt ttcgcagggg cgagaagtgt 7440
tccatggtcg gcacgctctg gccggtgagg cgcgcgcagt cgttgacgct ctatacacac 7500
acaaaaacga aagcgtttac agggctttcg ttctgtagcc tggaggaaag taaatgggtt 7560
gggttgcggt gtgccccggt tcgagaccaa gctgagctcg gccggctgaa gccgcagcta 7620
acgtggtatt ggcagtcccg tctcgaccca ggccctgtat cctccaggat acggtcgaga 7680
gcccttttgc tttcttggcc aagcgcccgt ggcgcgatct gggatagatg gtcgcgatga 7740
gaggacaaaa gcggctcgct tccgtagtct ggagaaacaa tcgccagggt tgcgttgcgg 7800
cgtaccccgg ttcgagcccc tatggcggct tggatcggcc ggaaccgcgg ctaacgtggg 7860
ctgtggcagc cccgtcctca ggaccccgcc agccgacttc tccagttacg ggagcgagcc 7920
ccttttgttt tttatttttt agatgcatcc cgtgctgcgg cagatgcgcc cctcgccccg 7980
gcccgatcag cagcagcaac agcaggcatg cagacccccc tctcctctac ccgccccggt 8040
caccacggcc gcggcggccg tgtccggcgc ggggggcgcg ctggagtcag atgagccacc 8100
gcggcggcga cctaggcagt atctggactt ggaagagggc gagggactgg cgcggctggg 8160
ggcgagctct ccagagcgcc acccgcgggt gcagttgaaa agggacgcgc gcgaggcgta 8220
cctgccgcgg cagaacctgt ttcgcgaccg cgggggcgag gagcccgagg agatgcgaga 8280
ctgcaggttc caagcggggc gcgagctgcg ccgcgggttg gacagacagc gcctgctgcg 8340
cgaggaggac tttgagcccg acacgcagac gggcatcagc cccgcgcgcg cgcacgtggc 8400
cgcggccgac ctggtgaccg cctacgagca gacggtgaac caggagcgca acttccaaaa 8460
aagcttcaac aaccacgtgc gcacgctggt ggcgcgcgag gaggtgaccc tgggtctcat 8520
gcatctgtgg gacctggtgg aggcgatcgt gcagaacccc agcagcaagc ccctgaccgc 8580
gcagctgttc ctggtggtgc agcacagcag ggacaacgag gccttcaggg aggcgctgct 8640
gaacatcacc gagccggagg ggcgctggct cctggacctg ataaacatcc tgcagagcat 8700
agtggtgcag gagcgcagcc tgagcctggc cgagaaggtg gcggccatta actattctat 8760
gctgagcttg ggcaagttct acgcccgcaa gatctacaag accccctacg tgcccataga 8820
caaggaggtg aagatagaca gcttctacat gcgcatggcg ctaaaggtgc tgaccctgag 8880
cgacgacctg ggagtgtacc gcaacgagcg catccacaag gccgtgagcg ccagccggcg 8940
gcgcgagctg agcgaccgcg agctgatgca cagtctgcag cgcgcgctca ccggcgcggg 9000
cgagggcgac agggaggtcg agtcctactt cgacatgggg gctgacctgc actggcagcc 9060
gagccgccgc gccctggagg cggcgggggc gtatggcggc cccctggcgg ccgatgacga 9120
ggaagaggag gactatgagc tagaggaggg cgagtacctg gaggactgac ctggctggtg 9180
gtgttttggt atagatgcaa gatccgaacg tggcggaccc ggcggtccgg gcggcgctgc 9240
agagccagcc gtccggcatt aactcctctg acgactgggc cgcggccatg ggtcgcatca 9300
tggccctgac cgcgcgcaac cccgaggcct tcaggcagca gcctcaggct aaccggctgg 9360
cggccatctt ggaagcggta gtgcccgcgc gctccaaccc cacccacgag aaggtgctgg 9420
ccatagtcaa cgcgctggcg gagagcaggg ccatccgggc ggacgaggcc ggactggtgt 9480
acgatgcgct gctgcagcgg gtggcgcggt acaacagcgg caacgtgcag accaacctgg 9540
accgcctggt gacggacgtg cgcgaggccg tggcgcagcg cgagcgcttg catcaggacg 9600
gtaacctggg ctcgctggtg gcgctaaacg ccttcctcag cacccagccg gccaacgtac 9660
cgcgggggca ggaggactac accaacttct tgagcgcgct gcggctgatg gtgaccgagg 9720
tccctcagag cgaggtgtac cagtcggggc ccgactactt cttccagacc agcagacagg 9780
gcttgcaaac cgtgaacctg agccaggctt tcaagaacct gcgggggctg tggggagtga 9840
aagcgcccac cggcgaccga gctacggtgt ccagcctgct aacccccaac tcgcgcctgc 9900
tgctgctgct gatcgcgccc ttcacggaca gcgggagcgt ctcgcgggag acctatctgg 9960
gccacctgct gacgctgtac cgcgaggcca tcgggcaggc gcaggtggac gagcacacct 10020
tccaagagat caccagcgtg agccacgcgc tggggcagga ggacacgggc agcctgcagg 10080
cgaccctgaa ctacctgctg accaacaggc ggcagaagat tcccacgctg cacagcctga 10140
cccaggagga ggagcgcatc ttgcgctacg tgcagcagag cgtgagcctg aacctgatgc 10200
gcgacggcgt gacgcccagc gtggcgctgg acatgaccgc gcgcaacatg gaaccgggca 10260
tgtacgcttc ccagcggccg ttcatcaacc gcctgatgga ctacttgcat cgggcggcgg 10320
ccgtgaaccc cgagtacttc accaatgcca ttctgaatcc ccactggatg ccccctccgg 10380
gtttctacaa cggagacttc gaggtgcctg aggtcaatga tgggttcctc tgggatgaca 10440
tggatgacag tgtgttctcc cccaacccgc tgcgcgccgc gtctctgcga ttgaaggagg 10500
gctctgacag ggaaggaccg aggagtctgg cctcctccct ggctctgggg gcggtgggcg 10560
ccacgggcgc ggcggcgcgg ggcagcagcc ccttccccag cctggcagac tctctgaata 10620
gcgggcgggt gagcaggccc cgcttgctag gcgaggagga gtatctgaac aactccctgc 10680
tgcagcccgt gagggacaaa aacgctcagc gacagcagtt tcccaacaac gggatagaga 10740
gcctggtgga caagatgtcc agatggaaga catatgcgca ggagtacaag gagtgggagg 10800
accgccagcc gcggcccctg ccgcccccta gacagcgctg gcagcggcgc gcgtccaacc 10860
gccgctggag acagggaccc gaggacgatg atgactctgc agatgacagc agcgtgttgg 10920
acctgggcgg gagcgggaac cccttttcgc acctgcgccc acgcctgggc aagatgtttt 10980
aaaagagaaa aataaaactc accaaggcca tggcgacgag cgttggtttt ttgttccctt 11040
ccttagtatg cggcgcgcgg cgatgttcga ggaggggcct cccccctctt acgagagcgc 11100
gatgggaatt tctcctgcgg cgcccctgca gcctccctac gtgcctcctc ggtacctgca 11160
acctacaggg gggagaaata gcatctgtta ctctgagctg cagcccctgt acgataccac 11220
cagactgtac ctggtggaca acaagtccgc ggacgtggcc tccctgaact accagaacga 11280
ccacagcgat tttttgacca cggtgatcca aaacaacgac ttcaccccaa ccgaggccag 11340
tacccagacc ataaacctgg acaacaggtc gaactggggc ggcgacctga agaccatcct 11400
gcacaccaac atgcccaacg tgaacgagtt catgtttacc aactctttta aggcgcgggt 11460
tatggtggcg cgcgagcagg gggaggcgaa gtacgagtgg gtggacttca cgctgcccga 11520
gggcaactac tcagagacca tgactattga cctgatgaac aatgcgatcg tggaacacta 11580
cctgaaagtg ggcaggcaga acggggtgaa ggagagcgat atcggggtca agtttgacac 11640
cagaaacttt cgtctgggct gggaccccgt gaccgggctg gtcatgccgg gggtctacac 11700
caacgaggcc tttcatcccg atatagtgct cctgcccggc tgtggggtgg actttaccca 11760
gagccggctg agcaacctgc tgggcgttcg caagcggcaa cctttccagg agggtttcaa 11820
gatcacctat gaggatctgg aggggggcaa cattcccgcg ctccttgatc tggacgccta 11880
cgaggagagc ttgaaacccg aggagagcgc tggcgacagc ggcgagagtg gcgaggagca 11940
agccggcggc ggtggcagcg cgtcggtaga aaacgaaagt actcccgcag tggcggcaga 12000
cgctgcggag gtcgagccag aggccatgca gcaggacgca gaggagggcg cacaggaggg 12060
cgcgcaggag gacatgaacg atggggagat caggggagac actttcgcca cccggggcga 12120
agaaaaagag gcagaggcgg tggcggcgac ggtggaagcc gaaaccgagg cagaggcaga 12180
gcccaagacc gaagttatgg aagacatgaa tgatggagaa cgtaggggtg acacgtttgc 12240
cacccggggc gaagagaagg cggcggaggc agaagccgcg gctgaggagg cggctgcggc 12300
tgcggccgag gctgaggctg cggctgaggc taaggtcgaa gccgatgttg cggttgaggc 12360
tcaggctgag gaggaggagg cggcgactga agcagttaag gaaaaggccc aggcagagca 12420
ggaagagaaa aaacctgtca ttcaacctct aaaagaagat agcaaaaagc gcagttacaa 12480
cgtcatcgag ggcagcacct ttacccagta ccgcagctgg tacctggcgt acaactacgg 12540
cgacccggtc aagggggtgc gctcgtggac cctgctctgc acgccggacg tcacctgcgg 12600
ctccgagcag atgtattggt cgctgccaaa catgatgcaa gacccggtga ccttccgctc 12660
cacgcggcag gttagcaact tcccggtggt gggcgccgaa ctgctgcccg tgcactccaa 12720
gagtttttac aacgagcagg ccgtctactc ccagctgatc cgccaggcca cctctctgac 12780
ccacgtgttc aatcgctttc ccgagaacca gattttggcg cgcccgccgg cccccaccat 12840
caccaccgtc agtgaaaacg ttcctgccct cacagatcac gggacgctac cgctgcgcaa 12900
cagcatctca ggagtccagc gagtgaccat tactgacgcc agacgccgga cctgccccta 12960
cgtttacaag gccttgggca tagtctcgcc gcgcgtcctc tccagtcgca ctttttaaaa 13020
tacatctacc ctcacgcttc aaaatcatgt ccgtactcat ctcacccagc aacaacaccg 13080
gctgggggct gcgcgcgccc agcaagatgt ttggaggggc gaggaaacgc tccgagcagc 13140
acccagtgcg cgtgcgcggc cactaccgcg cgccctgggg agcgcacaag cgcgggcgcg 13200
cagggcgcac cactgtggac gacgccattg actccgtagt ggagcaggcg cgccactaca 13260
cacccggcgc gccgtccgcc cccgccgtgt ccaccgtgga cgaggcgatc gagagcgtgg 13320
tacagggcgc gcggcactat gccaacctta aaaatcgacg ccgtcgcgtg gctcgccgcc 13380
atcgccggag accccgggcc accgccgccg cgcgccttgc taaggctctg ctcaggcgcg 13440
ccaggcgaac tggccgccgg gccgccatga gggccgcacg gcgggctgcc gccagcgcgg 13500
ccgccgcggc cccacgggca cgaaggcgcg cggccgctgc cgccgccgcc gccatttcca 13560
gcttggcctc gacgcggcgc ggtaacatat actgggtgcg cgactcggta accggcacgc 13620
gggtacccgt gcgctttcgc cccccgcgga attagcacaa gacaacatac acactgagtc 13680
tcctgctgtt gtgtatccca gcggcgaccg tcagcagcgg cgacatgtcc aagcgcaaaa 13740
ttaaagaaga gatgctccag gtcatcgcgc cggagatcta tgggcccccg aagaaggagg 13800
aggatgatta caagccccgc aagctaaagc gggtcaaaaa gaaaaagaaa gatgatgacg 13860
ttgacgaggc ggtggagttt gtccgccgca tggcgcccag gcgccccgtg cagtggaagg 13920
gtcggcgcgt gcagcgagtc ctgcgccccg gcaccgcggt ggtctttacg cccggcgagc 13980
gttccacgcg cactttcaag cgggtgtacg atgaggtgta cggcgacgag gatctgttgg 14040
agcaggccaa ccatcgcttt ggggagtttg catatgggaa acggccccgc gagagcctaa 14100
aagaggacct gctggcgcta ccgctggacg agggcaatcc caccccgagt ctgaagccgg 14160
taaccctgca acaggtgctg cctttgagcg cgcccagcga gcagaagcga gggttgaagc 14220
gcgagggcgg ggacctggca cccaccgtgc agttgatggt gcccaagcgg cagaagctgg 14280
aggacgtgct ggagaaaatg aaagtagagc ccgggatcca gcccgaaatc aaggtccgcc 14340
ccatcaagca ggtggcgccc ggcgtgggag tccagaccgt ggacgttagg attcccacgg 14400
aggagatgga aacccaaacc gccactccct cttcggcggc tagcgccacc accggctccg 14460
cttcggtaga ggtgcagacg gacccctggc tagccgccgc cgccccggcc gccccccgtt 14520
cgcgcgggcg caagagaaat tatccagcgg ccagcgcgct catgccccag tacgcactgc 14580
atccatccat cgcgcccacc cccggctacc gcgggtactc gtaccgcccg cgcagatcag 14640
ccggcacccg cggccgccgc cgccgtgcga ccacaaccag ccgccgccgt cgccgccgcc 14700
gccagccagt gctgaccccc gtgtctgtaa ggaaggtggc tcgctcgggg agcacgctgg 14760
tggtgcccag agcgcgctac caccccagca ttgtttaaag ccggtctctg tatggttctt 14820
gcagatatgg ccctcacttg tcgcctccgc ttcccggtgc cgggataccg aggaagaact 14880
caccgccgca gaggcatggc gggcagtggt ctccgcggcg gccgtcgcca tcgccggcgc 14940
gcaaagagca ggcgcatgcg cggcggtgtg ctgcccttcc taatcccgct aatcgccgcg 15000
gcgatcggtg ccgtgcccgg gatcgcctcc gtggccctgc aggcgtccca gaaacattga 15060
ctcttgcaac cttgcaagct tgcatttttt ggaggaaaaa ataaaaagtc tagactctca 15120
cgctcgcttg gtcctgtgac tattttgtag aaaaaagatg gaagacatca actttgcgtc 15180
gctggccccg cgtcacggct cgcgcccgtt catgggagac tggacagata tcggcaccag 15240
caatatgagc ggtggcgcct tcagctgggg cagtctgtgg agtggcctta aaaattttgg 15300
ttccaccatt aagaactatg gcaacaaagc gtggaacagc agcacgggtc agatgctgag 15360
agacaagttg aaagagcaga acttccagga gaaggtggca cagggcctgg cctctggcat 15420
cagcggggtg gtggacatag ctaaccaggc cgtgcagaaa aagataaaca gtcatctgga 15480
cccccgccct caggtggagg aaacgcctcc agccatggag acggtgtctc ccgagggcaa 15540
aggcgaaaag cgcccgcggc ccgacaggga agagaccctg gtgtcacaca ccgaggagcc 15600
gccctcttac gaggaggcag tcaaggccgg cctgcccacc actcgcccca tagctcccat 15660
ggccaccggt gtggtgggtc acaggcaaca cacccccgcg acactagatc tgcccccgcc 15720
gtccgagccg actcgccagc caaaggcggt gacggtgccc gctccctcca cttccgccgc 15780
caacagagtg cctctgcgcc gcgctgcgag cggcccccgg gcctcgcgag tcagcggcaa 15840
ctggcagagc acactgaaca gcatcgtggg cctgggagtg aggagtgtga agcgccgccg 15900
ttgctactga atgagcaagc tagctaacgt gttgtatgtg tgtatgcgtc ctatgtcgcc 15960
gccagaggag ctgttgagcc gccggcgccg tctgcactcc agcgaatttc aagatggcga 16020
ccccatcgat gatgcctcag tggtcgtaca tgcacatctc gggccaggac gcttcggagt 16080
acctgagccc cgggctggtg cagttcgccc gcgccacaga cacctacttc aacatgagta 16140
acaagttcag gaaccccact gtggcgccca cccacgatgt gaccacggac cggtcgcagc 16200
gcctgacgct gcggttcatc cccgtggatc gggaggacac cgcttactct tacaaggcgc 16260
ggttcacgct ggccgtgggc gacaaccgcg tgctggacat ggcctccact tactttgaca 16320
tccggggggt gctggacagg ggccccactt tcaagcccta ctcgggcact gcctacaact 16380
ccctggcccc caagggcgct cccaattctt gcgagtggga ggaggaaaca caaaatgagg 16440
tacaagccaa tgaagaacaa ctagcagaag aagaggatga agaaatggct caagaggatc 16500
agcagcctac taaaaaaacc catgtatatg ctcaggcacc tctttctggc gaacagatta 16560
ccaaagatgg cttgcaaata ggagctgaag ttacaggaga aacatcaaag cccatttttg 16620
cagacaagac attccaacca gaacctcaga taggagagtc tcaatggaat gaggccgatg 16680
ctacagtagc aggaggtagg gttttgaaaa agactacccc tatgaaacct tgctatggat 16740
cctatgccag acctaccaat gccaatggag ggcaggggat acttgaggca aatgctaaag 16800
gggaactcga atctaaagtt gagatgcagt ttttctctaa caccacaact cttaatgtaa 16860
gagacggtga aaatggcctt aaaccaaaag tagtgctgta tagcgaagat gtcaacctgg 16920
aatcccctga cactcatctg tcttacaagc ccaaaaaaga tgatgttaat gccaaaatca 16980
tgttgggtca gcaagccatg cccaacagac ccaacctcat tggatttaga gataatttca 17040
ttgggctcat gtattacaac agcactggaa acatgggagt gctggcgggt caggcctctc 17100
agttgaatgc tgtggtggac ttgcaggata gaaacacgga actgtcatat cagcttatgc 17160
ttgattccat tggagataga accagatact tttccatgtg gaaccaggca gtggatagct 17220
atgacccaga tgttagaatc attgaaaacc atggggtgga ggatgagctg cccaactact 17280
gttttccctt gggcggtata ggaattacag atacatacca ggccataaaa gcagccaatg 17340
gtggagatgc tactacgtgg tctgctgata acacatttgc agaccgcaac gaaatagggg 17400
tgggaaacaa cttcgccatg gagatcaaca tccaggccaa cctctggaga aacttcctct 17460
atgcgaacgt gggactctac ctgccagaca agctcaagta caaccccacc aacgtggaca 17520
tctctgacaa ccccaacacc tatgactaca tgaacaagcg ggtggtggcc cccggcctgg 17580
tggactgctt tgtcaatgtg ggagccaggt ggtccctgga ctacatggac aacgtcaacc 17640
ccttcaacca ccaccgcaat gcgggtctgc gctaccgctc catgatcctg ggcaacgggc 17700
gctatgtgcc cttccacatc caggtacccc agaagttctt tgccatcaag aacctcctgc 17760
tcctgcccgg ctcctacacc tacgagtgga acttcaggaa ggatgtaaac atggtcctac 17820
agagctctct gggcaatgac cttagggtag atggggccag catcaagttt gacagcatca 17880
ccctctatgc tacatttttc cccatggccc acaacaccgc ctccacgctt gaggccatgc 17940
tgagaaacga caccaacgac cagtccttca atgactacct ctctggggcc aacatgctct 18000
acccaatccc agccaaggcc accaacgtgc ccatctccat cccctctcgc aactgggccg 18060
cctttagagg ctgggccttt acccgcctta agaccaagga gaccccctcc ctgggctcgg 18120
gttttgatcc ctactttgtt tactcgggat ccatccccta cctggatggc accttctacc 18180
tcaaccacac tttcaagaag atatccatca tgtatgactc ctccgtcagc tggccgggca 18240
acgaccgctt gctcaccccc aatgagttcg aggtcaagcg cgccgtggac ggcgagggct 18300
acaacgtggc ccagtgcaac atgaccaagg actggttcct ggtgcagatg ctggccaact 18360
acaacatagg ctaccagggc ttctacatcc cagagagcta caaggacagg atgtattcct 18420
tcttcagaaa tttccaaccc atgagccgac aggtggtgga cgagaccaat tacaaggact 18480
atcaggccat tggcatcacc caccagcaca acaactcggg tttcgtgggc tacctggcgc 18540
ccaccatgcg cgagggacag gcctaccccg ccaacttccc ctaccccctg ataggcaaga 18600
ccgcggtcga cagcgtcacc cagaaaaagt tcctctgcga ccgcaccctc tggcgcatcc 18660
ccttctctag caactttatg tccatgggtg cgctcacgga cctgggccaa aacctgcttt 18720
atgccaactc tgcccatgcg ctggacatga cttttgaggt ggaccccatg gacgagccca 18780
cccttctcta tattgtgttt gaagtgttcg acgtggtcag agtgcaccag ccgcaccgcg 18840
gtgtcatcga gaccgtgtac ctgcgtacgc ccttctcagc cggcaacgcc accacctaag 18900
gagacagcgc cgccgcctgc atgactggtt ccaccgagca agagctcagg gccatcgcca 18960
gagacctggg atgcggaccc tattttttgg gcacctatga caaacgcttc ccgggtttta 19020
tctcccgaga caagctcgcc tgcgccatcg tcaacacggc cgcgcgcgag accgggggcg 19080
tgcactggct ggcctttggc tgggacccgc gctctaaaac ttgctacctc tttgacccct 19140
ttggcttctc cgatcagcgc ctcaggcaga tttatgagtt tgagtacgag gggctgttgc 19200
gccgcagcgc gcttgcctcc tcgcccgacc gctgcatcac ccttgagaag tccaccgaga 19260
ccgtgcaggg gccccactcg gccgcctgcg gtctcttctg ttgcatgttt ttgcacgcct 19320
ttgtgcactg gcctcagagt cccatggatc gcaaccccac catgaacttg ctaaagggag 19380
tgcccaacgc catgctccag agcccccagg tcctgcccac cctgcgccgc aaccaggaac 19440
agctctaccg cttcctggag cgccactccc cctacttccg cagccacagc gcgcgcatcc 19500
ggggggccac ctctttttgc cacttgcaag aaaacatgca agacggaaaa tgatgtacag 19560
catgctttta ataaatgtaa agactgtgca ctttatttat acacgggctc tttctggtta 19620
tttattcaac accgccgtcg ccatctagaa atcgaaaggg ttctgccgcg cgtcgccgtg 19680
cgccacgggc agagacacgt tgcgatactg gaagcggctc gcccacttga actcgggcac 19740
caccatgcgg ggcagtggtt cctcggggaa attctcgctc cacagggtgc gggtcagctg 19800
cagcgcgctc aggaggtcgg gagccgagat cttgaagtcg cagttggggc cggaaccctg 19860
cgcgcgcgag ttgcggtaca cggggttgca gcactggaac accagcaggg ccggattatt 19920
cacgctggcc agcaggctct cgtcgctaat catgtcgctg tccagatcct ccgcgttgct 19980
cagggcgaat ggggtcatct tgcagacctg cctgcccagg aaaggcggga gcccaggctt 20040
gccgttacag tcgcagcgca ggggcattag caggtgccca cggcccgact gcgcctgcgg 20100
gtacaacgcg cgcatgaagg cttcgatctg cctaaaagcc acctgggtct tggctccctc 20160
cgaaaagaac atcccacagg acttgctgga gaactggttc gcgggacagc tggcatcgtg 20220
caggcagcag cgcgcgtcag tgttggcgat ctgcaccacg ttgcgacccc accggttttt 20280
cactatcttg gccttggaag cctgctcctt tagcgcgcgc tggccgttct cgctggtcac 20340
atccatctct atcacctgtt ccttgttgat catgtttgtc ccgtgcagac actttaggtc 20400
gccctccgtc tgggtgcagc ggtgctccca cagcgcgcaa ccggtgggct cccaattttt 20460
gtgggtcacc cccgcgtagg cctgcaggta ggcctgcagg aagcgcccca tcatggtcat 20520
aaaggtcttc tggctcgtaa aggtcagctg caggccgcga tgctcttcgt tcagccaggt 20580
cttgcagatg gcggccagcg cctcggtctg ctcgggcagc atcttaaaat ttgtcttcag 20640
gtcgttatcc acgtggtact tgtccatcat ggcacgcgcc gcctccatgc ccttctccca 20700
ggcggacacc atgggcaggc ttagggggtt tatcacttcc agcggcgagg acaccgtact 20760
ttcgatttct tcttcctccc cctcttcccg gcgcgcgccc ccgctgttgc gcgctcttac 20820
cgcctgcacc aaggggtcgt cttcaggcaa gcgccgcacc gagcgcttgc cgcccttgac 20880
ctgcttgatc agtaccggcg ggttgctgaa gcccaccatg gtcagcgccg cctgctcttc 20940
ttcgtcttcg ctgtctacca ctatttctgg ggaggggctt ctccgctctg cggcaaaggc 21000
ggcggatcgc ttcttttttt tcttgggagc cgccgcgatg gagtccgcca cggcgaccga 21060
ggtcgagggc gtggggctgg gggtgcgcgg caccagggcc tcgtcgccct cggactcttc 21120
ctctgactcc aggcggcggc ggagtcgctt ctttgggggc gcgcgcgtta gcggcggcgg 21180
agacggggac ggggacgggg acgggacgcc ctccacaggg ggcggtcttc gcgcagaccc 21240
gcggccgcgc tcgggggtct tctcgcgctg gtcttggtcc cgactggcca ttgtatcctc 21300
ctcctcctag gcagagagac ataaggagtc tatcatgcaa gtcgagaagg aggagagctt 21360
aaccaccccc tctgagaccg ccgtcgccgt cgcccccgct accgccgacg cgcccgccac 21420
accgagcgac acccccgcgg acccccccgc cgacgcaccc ctgttcgagg aagcggccgt 21480
ggagcaggac ccgggctttg tctcggcaga ggaggatttg caagaggagg aggataagga 21540
ggagaagccc tcagtgccaa aagatcataa agagcaagac gagcacgacg cagacgcaca 21600
ccagggtgaa gtcgggcggg gggacggagg gcatggcggc gccgactacc tagacgaagg 21660
aaacgacgtg ctcttgaagc acctgcatcg tcagtgcgcc attgtctgcg acgctctgca 21720
ggagcgcagc gaggtgcccc tcagcgtggc ggaggtcagc cgcgcctacg agctcagcct 21780
cttttccccc cgggtgcccc cccgccgccg cgaaaacggc acatgcgagc ccaacccgcg 21840
cctcaacttc taccccgcct ttgtggtgcc cgaggtcctg gccacctatc acatcttctt 21900
tcaaaattgc aagatcccca tctcgtgccg cgccaaccgt agccgcgccg ataagatgct 21960
ggccctgcgc cagggcgacc acatacctga tatcgccgct ttggaagatg tgccaaagat 22020
cttcgagggt ctgggtcgca acgagaagcg ggcagcaaac tctctgcaac aggaaaacag 22080
cgaaaatgag agtcacactg gagcgctggt ggagctggag ggcgacaacg cccgcctggc 22140
ggtgctcaag cgcagcatcg aggtcaccca ctttgcctac cccgcgctca acctgccccc 22200
caaagtcatg aacgcggtca tggacgggct aatcatgcgc cgcggccggc cccttgctcc 22260
agatgcaaac ttgcatgagg agaccgagga cggtcagccc gtggtcagcg acgagcagct 22320
gacgcgctgg ctggaaaccg cggaccccgc cgaactggag gagcggcgca agatgatgat 22380
ggccgcggtg ctggtcaccg tagagctgga gtgtctgcag cgcttcttcg gcgaccccga 22440
gatgcagaga aaggtcgagg agaccctaca ctacaccttc cgccagggct acgtgcgcca 22500
ggcttgcaag atctccaacg tggagctcag caacctggtg tcctacctgg gcatcttgca 22560
tgaaaaccgc cttgggcaga gcgtgctaca ctccaccctg cgcggggagg cgcgccgcga 22620
ctacgtgcgc gactgcgttt acctcttcct ctgctacacc tggcagacgg ccatgggggt 22680
ctggcagcag tgcctggagg agcgcaacct caaggagctg gagaagctcc tgcagcgcgc 22740
gctcaaagac ctctggacgg gctacaacga gcgctcggtg gccgccgcgc tggccgacct 22800
catcttcccc gagcgcctgc tcaaaactct ccagcagggg ctgcccgact tcaccagcca 22860
aagcatgttg caaaatttta ggaactttat cctggagcgt tctggcatcc tacccgccac 22920
ctgctgcgcc ctgcccagcg actttgtccc cctcgtgtac cgcgagtgcc ccccgccgct 22980
gtggggccac tgctacctgt tccaactggc caactacctg tcctaccacg cggacctcat 23040
ggaagactcc agcggcgagg ggctcatgga gtgccactgc cgctgcaacc tctgcacgcc 23100
ccaccgctcc ctggtctgca acacccaact gctcagcgag agtcagatta tcggtacctt 23160
cgagctacag ggtccgtcct cctcagacga gaagtccgcg gctccggggc taaaactcac 23220
tccggggctg tggacttccg cctacctgcg caaatttgta cctgaagact accacgccca 23280
cgagatcagg ttttacgagg accaatcccg cccgcccaag gcggagctga ccgcctgcgt 23340
catcacccag ggcgagatcc taggccaatt gcaagccatc caaaaagccc gccaagagtt 23400
tttgctgaga aagggtcggg gggtgtatct ggacccccag tcgggtgagg agctcaaccc 23460
ggttcccccg ctgccgccgc cgcgggacct tgcttcccag gataagcatc gccatggctc 23520
ccagaaagaa gcagcagcgg ccgccactgc cgccacccca cacgctggag gaagaggagg 23580
aatactggga cagtcaggca gaggaggttt cggacgagga ggagccggag acggagatgg 23640
aagagtggga ggaggacagc ttagacgagg aggcttccga agccgaagag gcaggcgcaa 23700
caccgtcacc ctcggccgca gccccctcgc aggcgccccc gaagtccgct cccagcatca 23760
gcagcaacag cagcgctata acctccgctc ctccaccgcc gcgacccacg gccgaccgca 23820
gacccaaccg tagatgggac accaccggaa ccggggccgg taagtcctcc gggaaaggca 23880
agcaagcgca gcgccaaggc taccgctcgt ggcgcgctca caagaacgcc atagtcgctt 23940
gcttgcaaga ctgcgggggg aacatctcct tcgcccgccg cttcctgctc ttccaccacg 24000
gtgtggcctt cccccgtaac gtcctgcatt actaccgtca tctctacagc ccctactgcg 24060
gcggcagtga gccagaggcg gccggcggca gcggcgcccg tttcggtgcc taggaagacc 24120
cagggcaaga cttcagccaa gaaactcgcg gcggccgcgg cgaacgcggt cgcgggggcc 24180
ctgcgcctga cggtgaacga acccctgtcg acccgcgaac tgaggaaccg aatcttcccc 24240
actctctatg ccatcttcca gcagagcaga gggcaggatc aggaactgaa agtaaaaaac 24300
aggtctctgc gctccctcac ccgcagctgt ctgtatcaca agagcgaaga ccagcttcgg 24360
cgcacgctgg aggacgctga ggcactcttc agcaaatact gcgcgctcac tcttaaggac 24420
tagctccgcg cccttctcga atttaggcgg gaacgcctac gtcatcgcag cgccgccgtc 24480
atgagcaagg acattcccac gccatacatg tggagctatc agccgcagat gggactcgcg 24540
gcgggcgcct cccaggatta ctccacccgc atgaactggc tcagtgccgg cccacacatg 24600
atctcacagg ttaatgacat ccgcacccat cgaaaccaaa tattggtgga gcaggcggca 24660
attaccacca cgccccgcaa taatcccaac cccagggagt ggcccgcgtc cctggtgtat 24720
caggaaattc ccggccccac caccgtacta cttccgcgtg attcccaggc cgaagtccaa 24780
atgactaact caggggcaca gctcgcgggc ggctgtcgtc acagggtgcg gcctcctcgc 24840
cagggtataa ctcacctgaa gatccgaggc agaggtattc agctcaacga cgagtcggtg 24900
agctcctcgc tcggtctcag acctgacggg accttccaga tagccggagc cggccgatct 24960
tccttcacgc cccgccaggc gtacctgact ctgcagagct cgtcctcggc gccgcgctcg 25020
ggcggcatcg ggactctcca gttcgtgcag gagtttgtgc cctcggtcta cttcaacccc 25080
ttctcgggct ctcccggtcg ctacccggac cagttcatcc cgaactttga cgccgcgagg 25140
gactcggtgg acggctacga ctgaatgtcg ggtggacccg gtgcagagca acttcgcctg 25200
aagcaccttg accactgccg ccgccctcag tgctttgccc gctgtcagac cggtgagttc 25260
cagtactttt ccctgcccga ctcgcacccg gacggcccgg cgcacggggt gcgctttttc 25320
atcccgagtc aggtccgctc taccctaatc agggagttca ccgcccgtcc cctactggcg 25380
gagttggaaa aggggccttc tatcctaacc attgcctgca tctgctctaa ccctggatta 25440
caccaagatc tttgctgtca tttgtgtgct gagtataata aaggctgaga tcagaatcta 25500
ctcgggctcc tgtcgccatc ctgtcaacgc caccgtccaa gcccggcccg atcagcccga 25560
ggtgaacctc acctgcggtc tgcaccggcg cctgaggaaa tacctagctt ggtactacaa 25620
cagcactccc tttgtggttt acaacagctt tgaccaggac ggggtctcac tgagggataa 25680
cctctcgaac ctgagctact ccatcaggaa gaacaacacc ctcgagctac ttcctcctta 25740
cctgcccggg acttaccagt gtgtcaccgg tccctgcacc cacacccacc tgttgatcgt 25800
aaacgactct cttccgagaa cagacctcaa taactcctct ccgcagttcc ccagaacagg 25860
aggtgagctc aggaaacccc gggtaaagaa gggtggacaa gagttaacac ttgtggggtt 25920
tctggtgtat gtgacgctgg tggtggctct tttgattaag gcttttcctt ccatgtctga 25980
actatccctc ttcttttatg aacaactcga ctagtgctaa cgggacccta cccaacgaat 26040
cgggattgaa tatcggtaac caggttgcag tttcactttt gattaccttc atagtcctct 26100
tcctgctagt gctgtcgctt ctgtgcctgc ggatcggggg ctgctgcatc cacgtttata 26160
tctggtgctg gctgtttaga aggttcggag accaccgcag gtagaataaa caaacctaga 26220
cctagaaatg gacggtctct gcagcgagca acgcatacta gagaggcgcc ggcaaaaagc 26280
agagctcgag cgtcttaaac aagagctcca agacgccgtg gccatacacc agtgcaaaaa 26340
agggctcttc tgtctggtaa aacaggccac gctcacctat gaaaaaacag gtgacaccca 26400
ccgcctagga tacaagctgc ccacacagcg ccaaaagttt gcccttatga taggtgaaca 26460
acccatcacc gtgacccagc actccgtgga gacagaaggc tgcattcatg ctccctgcag 26520
gggcgctgac tgcctctaca ccttgatcaa aaccctttgc ggtctcagag accttatccc 26580
tttcaattga tcataactgt aatcaataaa aaatcactta cttgaaatct gatagcaagc 26640
ctctgtccaa ttttttcagc aacacttcct tcccctcttc ccaactctgg tactctaggc 26700
gcctcctagc tgcaaacttc ctccacagtc tgaagggaat gtcagattcc tcctcctcct 26760
gtccctccgc acccacgatc ttcatgttgt tgcagatgaa gcgcaccaaa acgtctgacg 26820
agagcttcaa ccccgtgtac ccctatgaca cggaaaacgg tcctccctcc gtccctttcc 26880
tcacccctcc cttcgtgtct cccgatggat tccaagagag cccccccggg gtcctgtctc 26940
tgaacctggc cgagcccctg gtcacttccc acggcatgct cgccctgaaa atgggaagtg 27000
gcctctccct ggacgacgcc ggcaacctca cctctcaaga tgtcaccacc actacccctc 27060
ccctgaaaaa aaccaagacc aacctcagcc tagaaacctc agcccccctg actgtgagca 27120
cctcaggcgc cctcacccta gcagccgccg ttcccctggc ggtggccggc acctccctca 27180
ccatgcaatc agaggccccc ctgacagtcc aagatgcaaa actcaccctg gccaccaagg 27240
gccccctgac cgtgtctgaa ggcaaactgg ccttgcagac ctcggccccg ctgacggccg 27300
ctgacagcag cgccctcacc gttagcgcca caccacccat cagtgtaagc agtggaagtt 27360
tgggcttaga catggaagac cccatgtata ctcatgatgg aaaactggga ataagaattg 27420
gaggcccact gagagtagta gacagcctgc acacactgac tgtagttacc ggaaatggaa 27480
tagctgtaga taacaatgcc ctccaaacta gagttacggg cgccctgggt tatgacacat 27540
caggaaacct acaactgaga gccgcggggg gtatgcgaat tgatgcaaat ggccaactta 27600
tccttgatgt ggcataccca tttgatgctc aaaacaatct cagccttaga cttggtcagg 27660
gacccctgta tgtaaacaca gaccacaacc tagatttgaa ttgcaacaga ggtctgacca 27720
caactaccac caacaacaca aaaaaacttg aaactaaaat tggctcaggc ttagactatg 27780
ataccaatgg tgctgtcatt attaaacttg gtactggtgt aagctttgac agcacaggcg 27840
ccctaactgt gggaaacact ggcgatgata aactgactct gtggacaacc ccagacccat 27900
ctccaaattg cagaattcac gcagacaaag actgcaagtt tactctagtc ctaactaagt 27960
gtggaagtca aatcctggct tctgtcgccg ccctagcggt gtcaggaaat ctggcttcaa 28020
taacaggcac cgttgccagc gttaccatct ttctcagatt tgatcagaat ggagtgctta 28080
tggaaaactc ctccctagac aagcagtact ggaacttcag aaatggtaac tcaaccaatg 28140
ccacccccta caccaatgca gttgggttca tgccaaacct cgcagcatac cccaagacac 28200
agagccagac tgctaaaaac aacattgtaa gtcaggttta cttgaatggg gacaaatcca 28260
aacccatgac ccttaccatt accctcaatg gaactaatga atccagtgaa actagccagg 28320
tgagtcacta ctccatgtca tttacatggg cttgggagag tgggcaatat gccaccgaaa 28380
cctttgccac caattccttt accttctctt acattgctga acaataaaga aagcacagag 28440
atgcttgttt ttgatttcaa aattgtgtgc ttttatttat tttcaagctt acagtatttc 28500
cagtagtcat tcgaatagag cttaatgaaa ctgcatgaga acccttccac atagcttaaa 28560
ttatcaccag tgcaaatgga gaaaaatcaa catacctttt tatccagata tcacagaacc 28620
ctagtattca acctgccacc tccctcccaa cacacagagt acacagtcct ttctccccgg 28680
ctggccttaa aaagcatcat atcatgggta acagacatat tcttaggtgt tatattccac 28740
acggtttcct gtcgagccaa acgctcatca gtgatattaa taaactcccc gggcagctca 28800
cttaagttca tgtcgctgtc cagctgctga gccacaggct gctgtccaac ttgcggttgc 28860
ttaacgggcg gcgaaggaga agtccacgcc tacatggggg tagagtcata atcgtgcatc 28920
aggatagggc ggtggtgctg cagcagcgcg cgaataaact gctgccgccg ccgctccgtc 28980
ctgcaggaat acaacatggc agtggtctcc tcagcgatga ttcgcaccgc ccgcagcata 29040
aggcgccttg tcctccgggc acagcagcgc accctgatct cacttaaatc agcacagtaa 29100
ctgcagcaca gcaccacaat attgttcaaa atcccacagt gcaaggcgct gtatccaaag 29160
ctcatggcgg ggaccacaga acccacgtgg ccatcatacc acaagcgcag gtagattaag 29220
tggcgacccc tcataaacac gctggacata aacattacct cttttggcat gttgtaattc 29280
accacctccc ggtaccatat aaacctctga ttaaacatgg cgccatccac caccatccta 29340
aaccagctgg ccaaaacctg cccgccggct atacactgca gggaaccggg actggaacaa 29400
tgacagtgga gagcccagga ctcgtaacca tggatcatca tgctcgtcat gatatcaatg 29460
ttggcacaac acaggcacac gtgcatacac ttcctcagga ttacaagctc ctcccgcgtt 29520
agaaccatat cccagggaac aacccattcc tgaatcagcg taaatcccac actgcaggga 29580
agacctcgca cgtaactcac gttgtgcatt gtcaaagtgt tacattcggg cagcagcgga 29640
tgatcctcca gtatggtagc gcgggtttct gtctcaaaag gaggtagacg atccctactg 29700
tacggagtgc gccgagacaa ccgagatcgt gttggtcgta gtgtcatgcc aaatggaacg 29760
ccggacgtag tcatatttcc tccagcagaa ccaagtgcgc gcgtggcagc tatccttgcg 29820
tcttctgtct cgccgcctgc cccgctcggt gtagtagttg taatacagcc actccctcag 29880
accgtcaagg cgctccctgg cgtccggatc tataacaaca ccatcctgca gcgccgccct 29940
gatgacatcc accaccgtag agtatgccaa gcccagccag gaaatgcact cactttgaca 30000
gcgagagata ggaggagcgg gaagagatgg aagaaccatg atagtaaaag aacttttatt 30060
ccaatcgatc ctctacaatg tcaaagtgta gatctatcag atggcactgg tctcctccgc 30120
tgagtcgatc aaaaataaca gctaaaccac aaacaacacg attggtcaaa tgctgcacaa 30180
gggcttgcag cataaaatcg cctcgaaagt ccaccgcaag cataacatca aagccaccgc 30240
ccctatcatg atctatgata aaaaccccac agctatccac cagacccata tagttttcat 30300
ctctccatcg tgaaaaaata tttacaagct cctcctttaa atcacctcca accaattcaa 30360
aaagttgagc cagaccgccc tccaccttca ttttcagcat gcgcatcatg attgcaaaaa 30420
ttcaggctcc tcagacacct gtataagatt gagaagcgga acattaacat caatgtttcg 30480
ctcgcgaaga tcgcgcctca gtgcaagcat gatataatcc cacaggtcgg agcggatcag 30540
cgaggacatc tccccgccag gaaccaactc aacggagcct atgctgatta taatacgcat 30600
attcggggct atgctaacca gcacggcccc caaataggcg tactgcatag gcggcgacaa 30660
aaagtgaaca gtttgggtta aaaaatcagg caaacactcg cgcaaaaaag caagaacatc 30720
ataaccatgc tcatgcaaat agatgcaagt aagctcagga acgaccacag aaaaatgcac 30780
aatttttctc tcaaacatga ctgcgagccc tgcaaaaaat aaaaaagaaa cattacacaa 30840
gagtagcctg tcttacaatg ggatagacta ctctaaccaa cataagacgg gccacgacat 30900
cgcccgcgtg gccataaaaa aaattatccg tgtgattaaa aagaagcaca gatagctggc 30960
cagtcatatc cggagtcatc acgtgcgaac ccgtgtagac ccccgggttg gacacatcgg 31020
ccaaagaaag aaagcggcca atgtatcccg gaggaatgat aacactaaga cgaagataca 31080
acagaataac cccatggggg ggaataacaa agttagtagg tgaataaaaa cgataaacac 31140
ccgaaactcc ctcctgcgta ggcaaaatag cgccctcccc ttccaaaaca acatacagcg 31200
cttccacagc agccatgaca aaagactcaa aacactcaaa agactcagtc ttaccaggaa 31260
aataaaagca ctctcacagc accagcacta atcagagtgt gaagagggcc aagtgccgaa 31320
cgagtatata taggaattaa aaatgacgta aatgtgtaaa ggtcaaaaaa cgcccagaaa 31380
aatacacaga ccaacgcccg aaacgaaaac ccgcgaaaaa atacccagaa gttcctcaac 31440
aaccgccact tccgctttcc cacgatacgt cacttcctcg aaaatagcaa actacatttc 31500
ccacatgtac aaaaccgaaa ccactcccct tgtcaccgcc cacaacttac atcttaatta 31560
acaaacgtca aagcctacgt cagccgcccc gcctcgcccc gcccacctca ttatcatatt 31620
ggccacaatc caaaataagg tatattattg atgatgattt aaatattatg cggtgtgaaa 31680
taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca 31740
ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 31800
taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 31860
agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 31920
cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 31980
tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 32040
tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 32100
gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 32160
acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 32220
acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 32280
cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 32340
gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 32400
gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 32460
agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 32520
ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 32580
ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 32640
atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 32700
tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 32760
gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 32820
ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 32880
caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 32940
cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct 33000
cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 33060
cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 33120
agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 33180
tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 33240
agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac 33300
atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 33360
ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 33420
cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 33480
caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 33540
attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 33600
agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct 33660
aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc 33720
gtcttcaaga attgatttaa at 33742
<210> 11
<211> 31807
<212> DNA
<213> 人工序列
<220>
<223> BZ1/BZ28腺病毒载体
<400> 11
catcatcaat aatatacctt attttggatt gtggccaata tgataatgag gtgggcgggg 60
cgaggcgggg cgggtgacgt aggacgtgcg agtagggttg ggaggtgtgg cggaagtgtg 120
gcatttgtaa gtgggaggag cttacatgca agcttccgtc gcggaaaatg tgacgttttt 180
tatgagcgcc gcctacctcc ggaagtgcca attttcgcgc gctttttacc ggatatcgta 240
gtaattttgg gcgggaccat gtaagatttg gccattttcg cgcgaaaagt gaaacgggga 300
agtgaaaact gaataatagg gcgttagtca tagcgcgtaa tatttaccga gggccgaggg 360
actttgaccg attacgtgga ggactcgccc aggtgttttt tacgtgaatt tccgcgttcc 420
gggtcaaagt ctccgttttt attgtcaccg tcatttgacg cggagggtat ttaaacccgc 480
tgcgctccta gcgatcgctg atgagaccag gaccaggtgc cgaccctgcg agtgcggcgg 540
caagcacatg agaaatcagc ctgtgatgtt ggatgtgacc gaggagctta ggcctgacca 600
tctggtgctg gcctgcacca gggccgagtt tgggtctagc gatgaggata ccgattgagg 660
tgggtaaggt gggcgtggct agcagggtgg gcgtgtataa attgggggtc taaggggtct 720
ctctgtttgt cttgcaacag ccgccgccat gagcgacacc ggcaacagct ttgatggaag 780
catctttagc ccctatctga cagtgcgcat gcctcactgg gccggagtgc gtcagaatgt 840
gatgggttcc aacgtggatg gacgtcccgt tctgccttca aattcgtcta cgatggccta 900
cgcgaccgtg ggaggaactc cgctggacgc cgcgacctcc gccgccgcct ccgccgccgc 960
cgcgaccgcg cgcagcatgg ctacggacct ttacagctct ttggtggcga gcagcgcggc 1020
ctctcgcgcg tctgctcggg atgaaaaact gactgctctg ctgcttaaac tggaagactt 1080
gacccgggag ctgggtcaac tgacccagca ggtctccagc ttgcgtgaga gcagccttgc 1140
ctccccctaa tggcccataa tataaataaa agccagtctg tttggattaa gcaagtgtat 1200
gttctttatt taactctccg cgcgcggtaa gcccgggacc agcggtctcg gtcgtttagg 1260
gtgcggtgga ttctttccaa cacgtggtac aggtggctct ggatgtttag atacatgggc 1320
atgagtccat ccctggggtg gaggtagcac cactgcagag cttcgtgctc gggggtggtg 1380
ttgtatatga tccagtcgta gcaggagcgc tgggcgtggt gctgaaaaat gtccttaagc 1440
aagaggctta tagctagggg gaggcccttg gtgtaagtgt ttacaaatct gcttagctgg 1500
gaggggtgca tccgggggga tatgatgtgc atcttggact ggatttttag gttggctatg 1560
ttcccaccca gatcccttct gggattcatg ttgtgcagga ccaccagcac ggtatatcca 1620
gtgcacttgg gaaatttatc gtggagctta gacgggaatg catggaagaa cttggagacg 1680
cccttgtggc ctcccagatt ttccatacat tcgtccatga tgatggcaat gggcccgtgg 1740
gaagctgcct gagcaaaaac gtttctggca tcgctcacat cgtagttatg ttccagggtg 1800
aggtcatcat aggacatctt tacaaatcgg gggcggaggg tcccggactg ggggatgatg 1860
gtaccctcgg gccccggggc gtagttcccc tcacagatct gcatctccca ggctttcatt 1920
tcagagggag ggatcatatc cacctgcggg gcgatgaaaa agacagtttc tggcgcaggg 1980
gagattaact gggatgagag caggtttctg agcagctgtg actttccaca gccggtgggc 2040
ccatatatca cgcctatcac cggctgcagc tggtagttaa gagagctgca gctgccgtcc 2100
tcccggagca ggggggccac ctcgttgagc atatccctga cgtggatgtt ctccctgacc 2160
agttccgcca gaaggcgctc gccgcccagc gaaagcagct cttgcaagga agcaaaattt 2220
ttcagcggtt tcaggccatc ggccgtgggc atgtttttca gcgtctgggt cagcagctcc 2280
agcctgtccc agagctcggt gatgtgctct acggcatctc gatccagcag atctcctcgt 2340
ttcgcgggtt ggggcggctt tcgctgtagg gcaccagccg atgggcgtcc agcggggcca 2400
gagtcatgtc cttccatggg cgcagagtcc tcgtcagggt ggtctgggtc acggtgaagg 2460
ggtgcgctcc gggttgggca ctggccaggg tgcgcttgag gctggttctg ctggtgctga 2520
atcgctgccg ctcttcgccc tgcgcgtcgg ccaggtagca tttgaccatg gtctcgtagt 2580
cgagaccctc ggcggcgtgc cccttggcgc ggagctttcc cttggaggtg gcgccgcacg 2640
aggggcactg caggctcttc agggcgtaga gcttgggagc gagaaacacg gactctgggg 2700
agtaggcgtc cgcgccgcag gccgcgcaga ccgtctcgca ttccaccagc caagtgagtt 2760
ccgggcggtc agggtcaaaa accaggttgc ccccatgctt tttgatgcgt ttcttacctc 2820
ggctctccat gaggcggtgt cccttctcgg tgacgaagag gctgtccgtg tccccgtaga 2880
ccgacttcag gggcctgtct tccagcggag tgcctctgtc ctcctcgtag agaaactctg 2940
accactctga gacgaaggcc cgtgtccagg ccaggacgaa ggaggccacg tgggaggggt 3000
agcggtcgtt gtccactagc gggtccacct tctccagggt gtgcagacac atgtccccct 3060
cctccgcgtc cagaaaagtg attggcttat aggtgtagga cacgtgaccg ggggttcccg 3120
acgggggggt ataaaagggg gtgggcgccc tttcatcttc actctcttcc gcatcgctgt 3180
ctgcgagagc cagctgctgg ggtaagtatt ccctttcaaa ggcgggcatg acctcagcgc 3240
tcaggttgtc agtttctaaa aatgaggagg atttgatgtt cacctgtccg gaagtgatac 3300
ctttgagggt acctgggtcc atctggtcag aaaacactat ttttttgttg tcaagcttgg 3360
tggcgaacga cccgtagagg gcgttggaga gcagcttggc aatggagcgc agggtctggt 3420
ttttgtcgcg gtcggctcgc tccttggccg cgatgttgag ttgcacgtat tcgcgggcca 3480
cgcacttcca ctcggggaag acggtggtgc gctcgtctgg gatcaggcgc accctccagc 3540
cgcggttgtg cagggtgacc atgtcgacgc tggtggcgac ctcaccgcgc agacgctcgt 3600
tggtccagca gaggcggccg cctttgcgcg agcagaaggg gggtaggggg tccagctggt 3660
cctcgtttgg ggggtccgcg tcgatggtaa agaccccggg gagcaggcgc gggtcaaagt 3720
agtcgatctt gcaagcttgc atgtccagag cccgctgcca ttcgcgggcg gcgagcgcgc 3780
gctcgtaggg gttgaggggc gggccccagg gcatggggtg ggtgagcgcg gaggcgtaca 3840
tgccgcagat gtcatacacg tacaggggtt ccctgaggat accgaggtag gtggggtagc 3900
agcgcccccc gcggatgctg gcgcgcacgt agtcatagag ctcgtgggag ggggccagca 3960
tgttgagccc aaggttggtg cgctgggggc gctcggcgcg gaagacgatc tgtctgaaga 4020
tggcatggga gttggaggag atggtgggtc gctggaagac gttgaagctt gcttcttgca 4080
agcccacgga gtccctgacg aaggaggcgt aggactcgcg cagcttgtgc accagctcgg 4140
cggtgacctg gacgtcgagc gcgcagtagt cgagggtctc gcggatgatg tcatacttat 4200
cctccccctt ctttttccac agctcgcggt tgaggacgaa ctcttcgcgg tctttccagt 4260
actcttggag gggaaacccg tccgtgtccg aacggtaaga gcctagcatg tagaactggt 4320
tgacggcctg gtaggggcag cagcccttct ccacgggcag cgcgtaggcc tgcgccgcct 4380
tgcggaggga ggtgtgggta agggcgaaag tgtccctgac catgactttg aggtattgat 4440
gtctgaagtc tgtgtcatcg cagccgccct gttcccacag ggtgtagtcc gtgcgctttt 4500
tggagcgcgg gttgggcagg gagaaggtga ggtcattgaa gaggatcttc cccgctcgag 4560
gcatgaagtt tctggtgatg cgaaagggcc ctgggaccga ggagcggttg ttgatgacct 4620
gggcggccag gacgatctcg tcaaagccgt ttatgttgtg gcccacgatg tagagctcca 4680
ggaagcgggg ctggcccttg atggagggga gctttttaag ttcctcgtag gtaagctcct 4740
cgggcgattc caggccgtgc tcctccaggg cccagtcttg caagtgaggg ttggccgcca 4800
ggaaggatcg ccagaggtcg cgggccatga gggtctgcag gcggtcgcgg aaggttctga 4860
actgccgccc tacggccatc ttttcggggg tgatgcagta gaaggtgagg gggtctttct 4920
cccaggggtc ccatctgagc tcttgggcga ggtcgcgcgc ggcggcgacc agagcctcgt 4980
cgccccccag tttcatgacc agcatgaagg gcacgagctg cttgccaaag gctcccatcc 5040
aagtgtaggt ctctacatcg taggtgacaa agaggcgctc cgtgcgagga tgagagccga 5100
tcgggaagaa ctggatctcc cgccaccagt tggaggattg gctgttgatg tggtgaaagt 5160
agaagtcccg tctgcgggcc gagcactcgt gctggctttt gtaaaagcga ccgcagtact 5220
ggcagcgctg cacgggttgt atatcttgca cgaggtgaac ctggcgacct ctgacgagga 5280
agcgcagcgg gaatctaagt cccccgcctg gggtcccgtg tggctggtgg tcttctactt 5340
tggttgtctg gccgccagca tctgtctcct ggagggcgat ggtggagcag accaccacgc 5400
cgcgagagcc gcaggtccag atctcggcgc tcggcgggcg gagtttgatg acgacatcgc 5460
gcacattgga gctgtccatg gtctccagct cccgcggcgg caggtcagcc gggagttcct 5520
ggaggttcac ctcgcagaga cgggtcaagg cgcggacagt gttgagatgg tatctgattt 5580
caaggggcgt gttggaggcg gagtcgatgg cttgcagaag gccgcagccc cggggggcca 5640
cgatggttcc ccgcggggcg cgaggggagg cggaagctgg gggtgtgttc agaagcggtg 5700
acgcgggcgg gcccccggag gtaggggggg ttccggcccc acaggcatgg gcggcagggg 5760
cacgtcttcg ccgcgcgcgg gcaggggctg gtgctggctc cggagagcgc ttgcgtgcgc 5820
gacgacgcga cggttggtgt cctgtatctg gcgcctctga gtgaagacca cgggtcccgt 5880
gaccttgaac ctgaaagaga gttcgacaga atcaatctcg gcatcgttga cagcggcctg 5940
gcgcaggatc tcctgcacgt cgcccgagtt gtcctggtag gcgatctctg ccatgaactg 6000
ctcgatctct tcctcctgga gatctcctcg tccggcgcgc tccacggtgg ccgccaggtc 6060
gttggagatg cgacccatga gctgcgagaa ggcgttgagg ccgccctcgt tccagacccg 6120
gctgtagacc acgcccccct cggcgtcgcg ggcgcgcatg accacctggg ccaggttgag 6180
ctccacgtgt cgcgtgaaga cggcgtagtt gcgcaggcgc tggaaaaggt agttcagggt 6240
ggtggcggtg tgttcggcga cgaagaagta catgacccag cgccgcaacg tggattcatt 6300
gatgtccccc aaggcctcca ggcgctccat ggcctcgtag aagtccacgg cgaagttgaa 6360
aaactgggag ttgcgagcgg acacggtcaa ctcctcctcc agaagacgga tgagctcggc 6420
gacagtgtcg cgcacctcgc gctcgaaggc cacggggggc gcttcttcct cttccacctc 6480
ttcttccatg attgcttctt cttcctcagc cgggacggga gggggcggcg gcgggggagg 6540
ggcgcggcgg cggcggcggc gcaccggcag gcggtcgatg aagcgctcga tcatctcccc 6600
ccgcatgcgg cgcatggtct cggtgacggc gcggccgttc tcccgggggc gcagctcaaa 6660
gacgccgcct ctcatctcgc cgcggggcgg gcggccgtga ggtagcgaga cggcgctgac 6720
tatgcatctt aacaattgct gtgtaggtac gccgccaagg gacctgattg agtccagatc 6780
caccggatcc gaaaaccttt ggaggaaagc gtctatccag tcgcagtcgc aaggtaggct 6840
gagcaccgtg gcgggcgggg gcgggtcggg agagttcctg gcggagatgc tgctgatgat 6900
gtaattaaag taggcggtct tgagaaggcg gatggtggac aggagcacca tgtctttggg 6960
tccggcctgt tggatgcgga ggcggtcggc catgccccag gcctcgttct gacaccggcg 7020
caggtctttg tagtagtctt gcatgagtct ttccaccggc acctcttctc cttcctcttc 7080
tccatctcgc cggtggtttc tcgcgccgcc catgcgcgtg accccaaagc ccctgagcgg 7140
ctgcagcagg gccaggtcgg cgaccacgcg ctcggccaag atggcctgct gtacctgagt 7200
gagggtcctc tcgaagtcat ccatgtccac gaagcggtgg taggcgcccg tgttgatggt 7260
gtaggtgcag ttggccatga cggaccagtt gacggtctgg tgtcccggct gcgagagctc 7320
cgtgtaccgc aggcgcgaga aggcgcggga atcgaacacg tagtcgttgc aagtccgcac 7380
cagatactgg tagcccacca ggaagtgcgg cggaggttgg cgatagaggg gccagcgctg 7440
ggtggcgggg gcgccgggcg ccaggtcttc cagcatgagg cggtggtatc cgtagatgta 7500
cctggacatc caggtgatgc cggcggcggt ggtggtggcg cgcgcgtagt cgcggacccg 7560
gttccagatg tttcgcaggg gcgagaagtg ttccatggtc ggcacgctct ggccggtgag 7620
gcgcgcgcag tcgttgacgc tctatacaca cacaaaaacg aaagcgttta cagggctttc 7680
gttctgtagc ctggaggaaa gtaaatgggt tgggttgcgg tgtgccccgg ttcgagacca 7740
agctgagctc ggccggctga agccgcagct aacgtggtat tggcagtccc gtctcgaccc 7800
aggccctgta tcctccagga tacggtcgag agcccttttg ctttcttggc caagcgcccg 7860
tggcgcgatc tgggatagat ggtcgcgatg agaggacaaa agcggctcgc ttccgtagtc 7920
tggagaaaca atcgccaggg ttgcgttgcg gcgtaccccg gttcgagccc ctatggcggc 7980
ttggatcggc cggaaccgcg gctaacgtgg gctgtggcag ccccgtcctc aggaccccgc 8040
cagccgactt ctccagttac gggagcgagc cccttttgtt ttttattttt tagatgcatc 8100
ccgtgctgcg gcagatgcgc ccctcgcccc ggcccgatca gcagcagcaa cagcaggcat 8160
gcagaccccc ctctcctcta cccgccccgg tcaccacggc cgcggcggcc gtgtccggcg 8220
cggggggcgc gctggagtca gatgagccac cgcggcggcg acctaggcag tatctggact 8280
tggaagaggg cgagggactg gcgcggctgg gggcgagctc tccagagcgc cacccgcggg 8340
tgcagttgaa aagggacgcg cgcgaggcgt acctgccgcg gcagaacctg tttcgcgacc 8400
gcgggggcga ggagcccgag gagatgcgag actgcaggtt ccaagcgggg cgcgagctgc 8460
gccgcgggtt ggacagacag cgcctgctgc gcgaggagga ctttgagccc gacacgcaga 8520
cgggcatcag ccccgcgcgc gcgcacgtgg ccgcggccga cctggtgacc gcctacgagc 8580
agacggtgaa ccaggagcgc aacttccaaa aaagcttcaa caaccacgtg cgcacgctgg 8640
tggcgcgcga ggaggtgacc ctgggtctca tgcatctgtg ggacctggtg gaggcgatcg 8700
tgcagaaccc cagcagcaag cccctgaccg cgcagctgtt cctggtggtg cagcacagca 8760
gggacaacga ggccttcagg gaggcgctgc tgaacatcac cgagccggag gggcgctggc 8820
tcctggacct gataaacatc ctgcagagca tagtggtgca ggagcgcagc ctgagcctgg 8880
ccgagaaggt ggcggccatt aactattcta tgctgagctt gggcaagttc tacgcccgca 8940
agatctacaa gaccccctac gtgcccatag acaaggaggt gaagatagac agcttctaca 9000
tgcgcatggc gctaaaggtg ctgaccctga gcgacgacct gggagtgtac cgcaacgagc 9060
gcatccacaa ggccgtgagc gccagccggc ggcgcgagct gagcgaccgc gagctgatgc 9120
acagtctgca gcgcgcgctc accggcgcgg gcgagggcga cagggaggtc gagtcctact 9180
tcgacatggg ggctgacctg cactggcagc cgagccgccg cgccctggag gcggcggggg 9240
cgtatggcgg ccccctggcg gccgatgacg aggaagagga ggactatgag ctagaggagg 9300
gcgagtacct ggaggactga cctggctggt ggtgttttgg tatagatgca agatccgaac 9360
gtggcggacc cggcggtccg ggcggcgctg cagagccagc cgtccggcat taactcctct 9420
gacgactggg ccgcggccat gggtcgcatc atggccctga ccgcgcgcaa ccccgaggcc 9480
ttcaggcagc agcctcaggc taaccggctg gcggccatct tggaagcggt agtgcccgcg 9540
cgctccaacc ccacccacga gaaggtgctg gccatagtca acgcgctggc ggagagcagg 9600
gccatccggg cggacgaggc cggactggtg tacgatgcgc tgctgcagcg ggtggcgcgg 9660
tacaacagcg gcaacgtgca gaccaacctg gaccgcctgg tgacggacgt gcgcgaggcc 9720
gtggcgcagc gcgagcgctt gcatcaggac ggtaacctgg gctcgctggt ggcgctaaac 9780
gccttcctca gcacccagcc ggccaacgta ccgcgggggc aggaggacta caccaacttc 9840
ttgagcgcgc tgcggctgat ggtgaccgag gtccctcaga gcgaggtgta ccagtcgggg 9900
cccgactact tcttccagac cagcagacag ggcttgcaaa ccgtgaacct gagccaggct 9960
ttcaagaacc tgcgggggct gtggggagtg aaagcgccca ccggcgaccg agctacggtg 10020
tccagcctgc taacccccaa ctcgcgcctg ctgctgctgc tgatcgcgcc cttcacggac 10080
agcgggagcg tctcgcggga gacctatctg ggccacctgc tgacgctgta ccgcgaggcc 10140
atcgggcagg cgcaggtgga cgagcacacc ttccaagaga tcaccagcgt gagccacgcg 10200
ctggggcagg aggacacggg cagcctgcag gcgaccctga actacctgct gaccaacagg 10260
cggcagaaga ttcccacgct gcacagcctg acccaggagg aggagcgcat cttgcgctac 10320
gtgcagcaga gcgtgagcct gaacctgatg cgcgacggcg tgacgcccag cgtggcgctg 10380
gacatgaccg cgcgcaacat ggaaccgggc atgtacgctt cccagcggcc gttcatcaac 10440
cgcctgatgg actacttgca tcgggcggcg gccgtgaacc ccgagtactt caccaatgcc 10500
attctgaatc cccactggat gccccctccg ggtttctaca acggagactt cgaggtgcct 10560
gaggtcaatg atgggttcct ctgggatgac atggatgaca gtgtgttctc ccccaacccg 10620
ctgcgcgccg cgtctctgcg attgaaggag ggctctgaca gggaaggacc gaggagtctg 10680
gcctcctccc tggctctggg ggcggtgggc gccacgggcg cggcggcgcg gggcagcagc 10740
cccttcccca gcctggcaga ctctctgaat agcgggcggg tgagcaggcc ccgcttgcta 10800
ggcgaggagg agtatctgaa caactccctg ctgcagcccg tgagggacaa aaacgctcag 10860
cgacagcagt ttcccaacaa cgggatagag agcctggtgg acaagatgtc cagatggaag 10920
acatatgcgc aggagtacaa ggagtgggag gaccgccagc cgcggcccct gccgccccct 10980
agacagcgct ggcagcggcg cgcgtccaac cgccgctgga gacagggacc cgaggacgat 11040
gatgactctg cagatgacag cagcgtgttg gacctgggcg ggagcgggaa ccccttttcg 11100
cacctgcgcc cacgcctggg caagatgttt taaaagagaa aaataaaact caccaaggcc 11160
atggcgacga gcgttggttt tttgttccct tccttagtat gcggcgcgcg gcgatgttcg 11220
aggaggggcc tcccccctct tacgagagcg cgatgggaat ttctcctgcg gcgcccctgc 11280
agcctcccta cgtgcctcct cggtacctgc aacctacagg ggggagaaat agcatctgtt 11340
actctgagct gcagcccctg tacgatacca ccagactgta cctggtggac aacaagtccg 11400
cggacgtggc ctccctgaac taccagaacg accacagcga ttttttgacc acggtgatcc 11460
aaaacaacga cttcacccca accgaggcca gtacccagac cataaacctg gacaacaggt 11520
cgaactgggg cggcgacctg aagaccatcc tgcacaccaa catgcccaac gtgaacgagt 11580
tcatgtttac caactctttt aaggcgcggg ttatggtggc gcgcgagcag ggggaggcga 11640
agtacgagtg ggtggacttc acgctgcccg agggcaacta ctcagagacc atgactattg 11700
acctgatgaa caatgcgatc gtggaacact acctgaaagt gggcaggcag aacggggtga 11760
aggagagcga tatcggggtc aagtttgaca ccagaaactt tcgtctgggc tgggaccccg 11820
tgaccgggct ggtcatgccg ggggtctaca ccaacgaggc ctttcatccc gatatagtgc 11880
tcctgcccgg ctgtggggtg gactttaccc agagccggct gagcaacctg ctgggcgttc 11940
gcaagcggca acctttccag gagggtttca agatcaccta tgaggatctg gaggggggca 12000
acattcccgc gctccttgat ctggacgcct acgaggagag cttgaaaccc gaggagagcg 12060
ctggcgacag cggcgagagt ggcgaggagc aagccggcgg cggtggcagc gcgtcggtag 12120
aaaacgaaag tactcccgca gtggcggcag acgctgcgga ggtcgagcca gaggccatgc 12180
agcaggacgc agaggagggc gcacaggagg gcgcgcagga ggacatgaac gatggggaga 12240
tcaggggaga cactttcgcc acccggggcg aagaaaaaga ggcagaggcg gtggcggcga 12300
cggtggaagc cgaaaccgag gcagaggcag agcccaagac cgaagttatg gaagacatga 12360
atgatggaga acgtaggggt gacacgtttg ccacccgggg cgaagagaag gcggcggagg 12420
cagaagccgc ggctgaggag gcggctgcgg ctgcggccga ggctgaggct gcggctgagg 12480
ctaaggtcga agccgatgtt gcggttgagg ctcaggctga ggaggaggag gcggcgactg 12540
aagcagttaa ggaaaaggcc caggcagagc aggaagagaa aaaacctgtc attcaacctc 12600
taaaagaaga tagcaaaaag cgcagttaca acgtcatcga gggcagcacc tttacccagt 12660
accgcagctg gtacctggcg tacaactacg gcgacccggt caagggggtg cgctcgtgga 12720
ccctgctctg cacgccggac gtcacctgcg gctccgagca gatgtattgg tcgctgccaa 12780
acatgatgca agacccggtg accttccgct ccacgcggca ggttagcaac ttcccggtgg 12840
tgggcgccga actgctgccc gtgcactcca agagttttta caacgagcag gccgtctact 12900
cccagctgat ccgccaggcc acctctctga cccacgtgtt caatcgcttt cccgagaacc 12960
agattttggc gcgcccgccg gcccccacca tcaccaccgt cagtgaaaac gttcctgccc 13020
tcacagatca cgggacgcta ccgctgcgca acagcatctc aggagtccag cgagtgacca 13080
ttactgacgc cagacgccgg acctgcccct acgtttacaa ggccttgggc atagtctcgc 13140
cgcgcgtcct ctccagtcgc actttttaaa atacatctac cctcacgctt caaaatcatg 13200
tccgtactca tctcacccag caacaacacc ggctgggggc tgcgcgcgcc cagcaagatg 13260
tttggagggg cgaggaaacg ctccgagcag cacccagtgc gcgtgcgcgg ccactaccgc 13320
gcgccctggg gagcgcacaa gcgcgggcgc gcagggcgca ccactgtgga cgacgccatt 13380
gactccgtag tggagcaggc gcgccactac acacccggcg cgccgtccgc ccccgccgtg 13440
tccaccgtgg acgaggcgat cgagagcgtg gtacagggcg cgcggcacta tgccaacctt 13500
aaaaatcgac gccgtcgcgt ggctcgccgc catcgccgga gaccccgggc caccgccgcc 13560
gcgcgccttg ctaaggctct gctcaggcgc gccaggcgaa ctggccgccg ggccgccatg 13620
agggccgcac ggcgggctgc cgccagcgcg gccgccgcgg ccccacgggc acgaaggcgc 13680
gcggccgctg ccgccgccgc cgccatttcc agcttggcct cgacgcggcg cggtaacata 13740
tactgggtgc gcgactcggt aaccggcacg cgggtacccg tgcgctttcg ccccccgcgg 13800
aattagcaca agacaacata cacactgagt ctcctgctgt tgtgtatccc agcggcgacc 13860
gtcagcagcg gcgacatgtc caagcgcaaa attaaagaag agatgctcca ggtcatcgcg 13920
ccggagatct atgggccccc gaagaaggag gaggatgatt acaagccccg caagctaaag 13980
cgggtcaaaa agaaaaagaa agatgatgac gttgacgagg cggtggagtt tgtccgccgc 14040
atggcgccca ggcgccccgt gcagtggaag ggtcggcgcg tgcagcgagt cctgcgcccc 14100
ggcaccgcgg tggtctttac gcccggcgag cgttccacgc gcactttcaa gcgggtgtac 14160
gatgaggtgt acggcgacga ggatctgttg gagcaggcca accatcgctt tggggagttt 14220
gcatatggga aacggccccg cgagagccta aaagaggacc tgctggcgct accgctggac 14280
gagggcaatc ccaccccgag tctgaagccg gtaaccctgc aacaggtgct gcctttgagc 14340
gcgcccagcg agcagaagcg agggttgaag cgcgagggcg gggacctggc acccaccgtg 14400
cagttgatgg tgcccaagcg gcagaagctg gaggacgtgc tggagaaaat gaaagtagag 14460
cccgggatcc agcccgaaat caaggtccgc cccatcaagc aggtggcgcc cggcgtggga 14520
gtccagaccg tggacgttag gattcccacg gaggagatgg aaacccaaac cgccactccc 14580
tcttcggcgg ctagcgccac caccggctcc gcttcggtag aggtgcagac ggacccctgg 14640
ctagccgccg ccgccccggc cgccccccgt tcgcgcgggc gcaagagaaa ttatccagcg 14700
gccagcgcgc tcatgcccca gtacgcactg catccatcca tcgcgcccac ccccggctac 14760
cgcgggtact cgtaccgccc gcgcagatca gccggcaccc gcggccgccg ccgccgtgcg 14820
accacaacca gccgccgccg tcgccgccgc cgccagccag tgctgacccc cgtgtctgta 14880
aggaaggtgg ctcgctcggg gagcacgctg gtggtgccca gagcgcgcta ccaccccagc 14940
attgtttaaa gccggtctct gtatggttct tgcagatatg gccctcactt gtcgcctccg 15000
cttcccggtg ccgggatacc gaggaagaac tcaccgccgc agaggcatgg cgggcagtgg 15060
tctccgcggc ggccgtcgcc atcgccggcg cgcaaagagc aggcgcatgc gcggcggtgt 15120
gctgcccttc ctaatcccgc taatcgccgc ggcgatcggt gccgtgcccg ggatcgcctc 15180
cgtggccctg caggcgtccc agaaacattg actcttgcaa ccttgcaagc ttgcattttt 15240
tggaggaaaa aataaaaagt ctagactctc acgctcgctt ggtcctgtga ctattttgta 15300
gaaaaaagat ggaagacatc aactttgcgt cgctggcccc gcgtcacggc tcgcgcccgt 15360
tcatgggaga ctggacagat atcggcacca gcaatatgag cggtggcgcc ttcagctggg 15420
gcagtctgtg gagtggcctt aaaaattttg gttccaccat taagaactat ggcaacaaag 15480
cgtggaacag cagcacgggt cagatgctga gagacaagtt gaaagagcag aacttccagg 15540
agaaggtggc acagggcctg gcctctggca tcagcggggt ggtggacata gctaaccagg 15600
ccgtgcagaa aaagataaac agtcatctgg acccccgccc tcaggtggag gaaacgcctc 15660
cagccatgga gacggtgtct cccgagggca aaggcgaaaa gcgcccgcgg cccgacaggg 15720
aagagaccct ggtgtcacac accgaggagc cgccctctta cgaggaggca gtcaaggccg 15780
gcctgcccac cactcgcccc atagctccca tggccaccgg tgtggtgggt cacaggcaac 15840
acacccccgc gacactagat ctgcccccgc cgtccgagcc gactcgccag ccaaaggcgg 15900
tgacggtgcc cgctccctcc acttccgccg ccaacagagt gcctctgcgc cgcgctgcga 15960
gcggcccccg ggcctcgcga gtcagcggca actggcagag cacactgaac agcatcgtgg 16020
gcctgggagt gaggagtgtg aagcgccgcc gttgctactg aatgagcaag ctagctaacg 16080
tgttgtatgt gtgtatgcgt cctatgtcgc cgccagagga gctgttgagc cgccggcgcc 16140
gtctgcactc cagcgaattt caagatggcg accccatcga tgatgcctca gtggtcgtac 16200
atgcacatct cgggccagga cgcttcggag tacctgagcc ccgggctggt gcagttcgcc 16260
cgcgccacag acacctactt caacatgagt aacaagttca ggaaccccac tgtggcgccc 16320
acccacgatg tgaccacgga ccggtcgcag cgcctgacgc tgcggttcat ccccgtggat 16380
cgggaggaca ccgcttactc ttacaaggcg cggttcacgc tggccgtggg cgacaaccgc 16440
gtgctggaca tggcctccac ttactttgac atccgggggg tgctggacag gggccccact 16500
ttcaagccct actcgggcac tgcctacaac tccctggccc ccaagggcgc tcccaattct 16560
tgcgagtggg aggaggaaac acaaaatgag gtacaagcca atgaagaaca actagcagaa 16620
gaagaggatg aagaaatggc tcaagaggat cagcagccta ctaaaaaaac ccatgtatat 16680
gctcaggcac ctctttctgg cgaacagatt accaaagatg gcttgcaaat aggagctgaa 16740
gttacaggag aaacatcaaa gcccattttt gcagacaaga cattccaacc agaacctcag 16800
ataggagagt ctcaatggaa tgaggccgat gctacagtag caggaggtag ggttttgaaa 16860
aagactaccc ctatgaaacc ttgctatgga tcctatgcca gacctaccaa tgccaatgga 16920
gggcagggga tacttgaggc aaatgctaaa ggggaactcg aatctaaagt tgagatgcag 16980
tttttctcta acaccacaac tcttaatgta agagacggtg aaaatggcct taaaccaaaa 17040
gtagtgctgt atagcgaaga tgtcaacctg gaatcccctg acactcatct gtcttacaag 17100
cccaaaaaag atgatgttaa tgccaaaatc atgttgggtc agcaagccat gcccaacaga 17160
cccaacctca ttggatttag agataatttc attgggctca tgtattacaa cagcactgga 17220
aacatgggag tgctggcggg tcaggcctct cagttgaatg ctgtggtgga cttgcaggat 17280
agaaacacgg aactgtcata tcagcttatg cttgattcca ttggagatag aaccagatac 17340
ttttccatgt ggaaccaggc agtggatagc tatgacccag atgttagaat cattgaaaac 17400
catggggtgg aggatgagct gcccaactac tgttttccct tgggcggtat aggaattaca 17460
gatacatacc aggccataaa agcagccaat ggtggagatg ctactacgtg gtctgctgat 17520
aacacatttg cagaccgcaa cgaaataggg gtgggaaaca acttcgccat ggagatcaac 17580
atccaggcca acctctggag aaacttcctc tatgcgaacg tgggactcta cctgccagac 17640
aagctcaagt acaaccccac caacgtggac atctctgaca accccaacac ctatgactac 17700
atgaacaagc gggtggtggc ccccggcctg gtggactgct ttgtcaatgt gggagccagg 17760
tggtccctgg actacatgga caacgtcaac cccttcaacc accaccgcaa tgcgggtctg 17820
cgctaccgct ccatgatcct gggcaacggg cgctatgtgc ccttccacat ccaggtaccc 17880
cagaagttct ttgccatcaa gaacctcctg ctcctgcccg gctcctacac ctacgagtgg 17940
aacttcagga aggatgtaaa catggtccta cagagctctc tgggcaatga ccttagggta 18000
gatggggcca gcatcaagtt tgacagcatc accctctatg ctacattttt ccccatggcc 18060
cacaacaccg cctccacgct tgaggccatg ctgagaaacg acaccaacga ccagtccttc 18120
aatgactacc tctctggggc caacatgctc tacccaatcc cagccaaggc caccaacgtg 18180
cccatctcca tcccctctcg caactgggcc gcctttagag gctgggcctt tacccgcctt 18240
aagaccaagg agaccccctc cctgggctcg ggttttgatc cctactttgt ttactcggga 18300
tccatcccct acctggatgg caccttctac ctcaaccaca ctttcaagaa gatatccatc 18360
atgtatgact cctccgtcag ctggccgggc aacgaccgct tgctcacccc caatgagttc 18420
gaggtcaagc gcgccgtgga cggcgagggc tacaacgtgg cccagtgcaa catgaccaag 18480
gactggttcc tggtgcagat gctggccaac tacaacatag gctaccaggg cttctacatc 18540
ccagagagct acaaggacag gatgtattcc ttcttcagaa atttccaacc catgagccga 18600
caggtggtgg acgagaccaa ttacaaggac tatcaggcca ttggcatcac ccaccagcac 18660
aacaactcgg gtttcgtggg ctacctggcg cccaccatgc gcgagggaca ggcctacccc 18720
gccaacttcc cctaccccct gataggcaag accgcggtcg acagcgtcac ccagaaaaag 18780
ttcctctgcg accgcaccct ctggcgcatc cccttctcta gcaactttat gtccatgggt 18840
gcgctcacgg acctgggcca aaacctgctt tatgccaact ctgcccatgc gctggacatg 18900
acttttgagg tggaccccat ggacgagccc acccttctct atattgtgtt tgaagtgttc 18960
gacgtggtca gagtgcacca gccgcaccgc ggtgtcatcg agaccgtgta cctgcgtacg 19020
cccttctcag ccggcaacgc caccacctaa ggagacagcg ccgccgcctg catgactggt 19080
tccaccgagc aagagctcag ggccatcgcc agagacctgg gatgcggacc ctattttttg 19140
ggcacctatg acaaacgctt cccgggtttt atctcccgag acaagctcgc ctgcgccatc 19200
gtcaacacgg ccgcgcgcga gaccgggggc gtgcactggc tggcctttgg ctgggacccg 19260
cgctctaaaa cttgctacct ctttgacccc tttggcttct ccgatcagcg cctcaggcag 19320
atttatgagt ttgagtacga ggggctgttg cgccgcagcg cgcttgcctc ctcgcccgac 19380
cgctgcatca cccttgagaa gtccaccgag accgtgcagg ggccccactc ggccgcctgc 19440
ggtctcttct gttgcatgtt tttgcacgcc tttgtgcact ggcctcagag tcccatggat 19500
cgcaacccca ccatgaactt gctaaaggga gtgcccaacg ccatgctcca gagcccccag 19560
gtcctgccca ccctgcgccg caaccaggaa cagctctacc gcttcctgga gcgccactcc 19620
ccctacttcc gcagccacag cgcgcgcatc cggggggcca cctctttttg ccacttgcaa 19680
gaaaacatgc aagacggaaa atgatgtaca gcatgctttt aataaatgta aagactgtgc 19740
actttattta tacacgggct ctttctggtt atttattcaa caccgccgtc gccatctaga 19800
aatcgaaagg gttctgccgc gcgtcgccgt gcgccacggg cagagacacg ttgcgatact 19860
ggaagcggct cgcccacttg aactcgggca ccaccatgcg gggcagtggt tcctcgggga 19920
aattctcgct ccacagggtg cgggtcagct gcagcgcgct caggaggtcg ggagccgaga 19980
tcttgaagtc gcagttgggg ccggaaccct gcgcgcgcga gttgcggtac acggggttgc 20040
agcactggaa caccagcagg gccggattat tcacgctggc cagcaggctc tcgtcgctaa 20100
tcatgtcgct gtccagatcc tccgcgttgc tcagggcgaa tggggtcatc ttgcagacct 20160
gcctgcccag gaaaggcggg agcccaggct tgccgttaca gtcgcagcgc aggggcatta 20220
gcaggtgccc acggcccgac tgcgcctgcg ggtacaacgc gcgcatgaag gcttcgatct 20280
gcctaaaagc cacctgggtc ttggctccct ccgaaaagaa catcccacag gacttgctgg 20340
agaactggtt cgcgggacag ctggcatcgt gcaggcagca gcgcgcgtca gtgttggcga 20400
tctgcaccac gttgcgaccc caccggtttt tcactatctt ggccttggaa gcctgctcct 20460
ttagcgcgcg ctggccgttc tcgctggtca catccatctc tatcacctgt tccttgttga 20520
tcatgtttgt cccgtgcaga cactttaggt cgccctccgt ctgggtgcag cggtgctccc 20580
acagcgcgca accggtgggc tcccaatttt tgtgggtcac ccccgcgtag gcctgcaggt 20640
aggcctgcag gaagcgcccc atcatggtca taaaggtctt ctggctcgta aaggtcagct 20700
gcaggccgcg atgctcttcg ttcagccagg tcttgcagat ggcggccagc gcctcggtct 20760
gctcgggcag catcttaaaa tttgtcttca ggtcgttatc cacgtggtac ttgtccatca 20820
tggcacgcgc cgcctccatg cccttctccc aggcggacac catgggcagg cttagggggt 20880
ttatcacttc cagcggcgag gacaccgtac tttcgatttc ttcttcctcc ccctcttccc 20940
ggcgcgcgcc cccgctgttg cgcgctctta ccgcctgcac caaggggtcg tcttcaggca 21000
agcgccgcac cgagcgcttg ccgcccttga cctgcttgat cagtaccggc gggttgctga 21060
agcccaccat ggtcagcgcc gcctgctctt cttcgtcttc gctgtctacc actatttctg 21120
gggaggggct tctccgctct gcggcaaagg cggcggatcg cttctttttt ttcttgggag 21180
ccgccgcgat ggagtccgcc acggcgaccg aggtcgaggg cgtggggctg ggggtgcgcg 21240
gcaccagggc ctcgtcgccc tcggactctt cctctgactc caggcggcgg cggagtcgct 21300
tctttggggg cgcgcgcgtt agcggcggcg gagacgggga cggggacggg gacgggacgc 21360
cctccacagg gggcggtctt cgcgcagacc cgcggccgcg ctcgggggtc ttctcgcgct 21420
ggtcttggtc ccgactggcc attgtatcct cctcctccta ggcagagaga cataaggagt 21480
ctatcatgca agtcgagaag gaggagagct taaccacccc ctctgagacc gccgtcgccg 21540
tcgcccccgc taccgccgac gcgcccgcca caccgagcga cacccccgcg gacccccccg 21600
ccgacgcacc cctgttcgag gaagcggccg tggagcagga cccgggcttt gtctcggcag 21660
aggaggattt gcaagaggag gaggataagg aggagaagcc ctcagtgcca aaagatcata 21720
aagagcaaga cgagcacgac gcagacgcac accagggtga agtcgggcgg ggggacggag 21780
ggcatggcgg cgccgactac ctagacgaag gaaacgacgt gctcttgaag cacctgcatc 21840
gtcagtgcgc cattgtctgc gacgctctgc aggagcgcag cgaggtgccc ctcagcgtgg 21900
cggaggtcag ccgcgcctac gagctcagcc tcttttcccc ccgggtgccc ccccgccgcc 21960
gcgaaaacgg cacatgcgag cccaacccgc gcctcaactt ctaccccgcc tttgtggtgc 22020
ccgaggtcct ggccacctat cacatcttct ttcaaaattg caagatcccc atctcgtgcc 22080
gcgccaaccg tagccgcgcc gataagatgc tggccctgcg ccagggcgac cacatacctg 22140
atatcgccgc tttggaagat gtgccaaaga tcttcgaggg tctgggtcgc aacgagaagc 22200
gggcagcaaa ctctctgcaa caggaaaaca gcgaaaatga gagtcacact ggagcgctgg 22260
tggagctgga gggcgacaac gcccgcctgg cggtgctcaa gcgcagcatc gaggtcaccc 22320
actttgccta ccccgcgctc aacctgcccc ccaaagtcat gaacgcggtc atggacgggc 22380
taatcatgcg ccgcggccgg ccccttgctc cagatgcaaa cttgcatgag gagaccgagg 22440
acggtcagcc cgtggtcagc gacgagcagc tgacgcgctg gctggaaacc gcggaccccg 22500
ccgaactgga ggagcggcgc aagatgatga tggccgcggt gctggtcacc gtagagctgg 22560
agtgtctgca gcgcttcttc ggcgaccccg agatgcagag aaaggtcgag gagaccctac 22620
actacacctt ccgccagggc tacgtgcgcc aggcttgcaa gatctccaac gtggagctca 22680
gcaacctggt gtcctacctg ggcatcttgc atgaaaaccg ccttgggcag agcgtgctac 22740
actccaccct gcgcggggag gcgcgccgcg actacgtgcg cgactgcgtt tacctcttcc 22800
tctgctacac ctggcagacg gccatggggg tctggcagca gtgcctggag gagcgcaacc 22860
tcaaggagct ggagaagctc ctgcagcgcg cgctcaaaga cctctggacg ggctacaacg 22920
agcgctcggt ggccgccgcg ctggccgacc tcatcttccc cgagcgcctg ctcaaaactc 22980
tccagcaggg gctgcccgac ttcaccagcc aaagcatgtt gcaaaatttt aggaacttta 23040
tcctggagcg ttctggcatc ctacccgcca cctgctgcgc cctgcccagc gactttgtcc 23100
ccctcgtgta ccgcgagtgc cccccgccgc tgtggggcca ctgctacctg ttccaactgg 23160
ccaactacct gtcctaccac gcggacctca tggaagactc cagcggcgag gggctcatgg 23220
agtgccactg ccgctgcaac ctctgcacgc cccaccgctc cctggtctgc aacacccaac 23280
tgctcagcga gagtcagatt atcggtacct tcgagctaca gggtccgtcc tcctcagacg 23340
agaagtccgc ggctccgggg ctaaaactca ctccggggct gtggacttcc gcctacctgc 23400
gcaaatttgt acctgaagac taccacgccc acgagatcag gttttacgag gaccaatccc 23460
gcccgcccaa ggcggagctg accgcctgcg tcatcaccca gggcgagatc ctaggccaat 23520
tgcaagccat ccaaaaagcc cgccaagagt ttttgctgag aaagggtcgg ggggtgtatc 23580
tggaccccca gtcgggtgag gagctcaacc cggttccccc gctgccgccg ccgcgggacc 23640
ttgcttccca ggataagcat cgccatggct cccagaaaga agcagcagcg gccgccactg 23700
ccgccacccc acacgctgga ggaagaggag gaatactggg acagtcaggc agaggaggtt 23760
tcggacgagg aggagccgga gacggagatg gaagagtggg aggaggacag cttagacgag 23820
gaggcttccg aagccgaaga ggcaggcgca acaccgtcac cctcggccgc agccccctcg 23880
caggcgcccc cgaagtccgc tcccagcatc agcagcaaca gcagcgctat aacctccgct 23940
cctccaccgc cgcgacccac ggccgaccgc agacccaacc gtagatggga caccaccgga 24000
accggggccg gtaagtcctc cgggaaaggc aagcaagcgc agcgccaagg ctaccgctcg 24060
tggcgcgctc acaagaacgc catagtcgct tgcttgcaag actgcggggg gaacatctcc 24120
ttcgcccgcc gcttcctgct cttccaccac ggtgtggcct tcccccgtaa cgtcctgcat 24180
tactaccgtc atctctacag cccctactgc ggcggcagtg agccagaggc ggccggcggc 24240
agcggcgccc gtttcggtgc ctaggaagac ccagggcaag acttcagcca agaaactcgc 24300
ggcggccgcg gcgaacgcgg tcgcgggggc cctgcgcctg acggtgaacg aacccctgtc 24360
gacccgcgaa ctgaggaacc gaatcttccc cactctctat gccatcttcc agcagagcag 24420
agggcaggat caggaactga aagtaaaaaa caggtctctg cgctccctca cccgcagctg 24480
tctgtatcac aagagcgaag accagcttcg gcgcacgctg gaggacgctg aggcactctt 24540
cagcaaatac tgcgcgctca ctcttaagga ctagctccgc gcccttctcg aatttaggcg 24600
ggaacgccta cgtcatcgca gcgccgccgt catgagcaag gacattccca cgccatacat 24660
gtggagctat cagccgcaga tgggactcgc ggcgggcgcc tcccaggatt actccacccg 24720
catgaactgg ctcagtgccg gcccacacat gatctcacag gttaatgaca tccgcaccca 24780
tcgaaaccaa atattggtgg agcaggcggc aattaccacc acgccccgca ataatcccaa 24840
ccccagggag tggcccgcgt ccctggtgta tcaggaaatt cccggcccca ccaccgtact 24900
acttccgcgt gattcccagg ccgaagtcca aatgactaac tcaggggcac agctcgcggg 24960
cggctgtcgt cacagggtgc ggcctcctcg ccagggtata actcacctga agatccgagg 25020
cagaggtatt cagctcaacg acgagtcggt gagctcctcg ctcggtctca gacctgacgg 25080
gaccttccag atagccggag ccggccgatc ttccttcacg ccccgccagg cgtacctgac 25140
tctgcagagc tcgtcctcgg cgccgcgctc gggcggcatc gggactctcc agttcgtgca 25200
ggagtttgtg ccctcggtct acttcaaccc cttctcgggc tctcccggtc gctacccgga 25260
ccagttcatc ccgaactttg acgccgcgag ggactcggtg gacggctacg actgaatgtc 25320
gggtggaccc ggtgcagagc aacttcgcct gaagcacctt gaccactgcc gccgccctca 25380
gtgctttgcc cgctgtcaga ccggtgagtt ccagtacttt tccctgcccg actcgcaccc 25440
ggacggcccg gcgcacgggg tgcgcttttt catcccgagt caggtccgct ctaccctaat 25500
cagggagttc accgcccgtc ccctactggc ggagttggaa aaggggcctt ctatcctaac 25560
cattgcctgc atctgctcta accctggatt acaccaagat ctttgctgtc atttgtgtgc 25620
tgagtataat aaaggctgag atcagaatct actcgggctc ctgtcgccat cctgtcaacg 25680
ccaccgtcca agcccggccc gatcagcccg aggtgaacct cacctgcggt ctgcaccggc 25740
gcctgaggaa atacctagct tggtactaca acagcactcc ctttgtggtt tacaacagct 25800
ttgaccagga cggggtctca ctgagggata acctctcgaa cctgagctac tccatcagga 25860
agaacaacac cctcgagcta cttcctcctt acctgcccgg gacttaccag tgtgtcaccg 25920
gtccctgcac ccacacccac ctgttgatcg taaacgactc tcttccgaga acagacctca 25980
ataactcctc tccgcagttc cccagaacag gaggtgagct caggaaaccc cgggtaaaga 26040
agggtggaca agagttaaca cttgtggggt ttctggtgta tgtgacgctg gtggtggctc 26100
ttttgattaa ggcttttcct tccatgtctg aactatccct cttcttttat gaacaactcg 26160
actagtgcta acgggaccct acccaacgaa tcgggattga atatcggtaa ccaggttgca 26220
gtttcacttt tgattacctt catagtcctc ttcctgctag tgctgtcgct tctgtgcctg 26280
cggatcgggg gctgctgcat ccacgtttat atctggtgct ggctgtttag aaggttcgga 26340
gaccaccgca ggtagaataa acaaacctag acctagaaat ggacggtctc tgcagcgagc 26400
aacgcatact agagaggcgc cggcaaaaag cagagctcga gcgtcttaaa caagagctcc 26460
aagacgccgt ggccatacac cagtgcaaaa aagggctctt ctgtctggta aaacaggcca 26520
cgctcaccta tgaaaaaaca ggtgacaccc accgcctagg atacaagctg cccacacagc 26580
gccaaaagtt tgcccttatg ataggtgaac aacccatcac cgtgacccag cactccgtgg 26640
agacagaagg ctgcattcat gctccctgca ggggcgctga ctgcctctac accttgatca 26700
aaaccctttg cggtctcaga gaccttatcc ctttcaattg atcataactg taatcaataa 26760
aaaatcactt acttgaaatc tgatagcaag cctctgtcca attttttcag caacacttcc 26820
ttcccctctt cccaactctg gtactctagg cgcctcctag ctgcaaactt cctccacagt 26880
ctgaagggaa tgtcagattc ctcctcctcc tgtccctccg cacccacgat cttcatgttg 26940
ttgcagatga agcgcaccaa aacgtctgac gagagcttca accccgtgta cccctatgac 27000
acggaaaacg gtcctccctc cgtccctttc ctcacccctc ccttcgtgtc tcccgatgga 27060
ttccaagaga gcccccccgg ggtcctgtct ctgaacctgg ccgagcccct ggtcacttcc 27120
cacggcatgc tcgccctgaa aatgggaagt ggcctctccc tggacgacgc cggcaacctc 27180
acctctcaag atgtcaccac cactacccct cccctgaaaa aaaccaagac caacctcagc 27240
ctagaaacct cagcccccct gactgtgagc acctcaggcg ccctcaccct agcagccgcc 27300
gttcccctgg cggtggccgg cacctccctc accatgcaat cagaggcccc cctgacagtc 27360
caagatgcaa aactcaccct ggccaccaag ggccccctga ccgtgtctga aggcaaactg 27420
gccttgcaga cctcggcccc gctgacggcc gctgacagca gcgccctcac cgttagcgcc 27480
acaccaccca tcagtgtaag cagtggaagt ttgggcttag acatggaaga ccccatgtat 27540
actcatgatg gaaaactggg aataagaatt ggaggcccac tgagagtagt agacagcctg 27600
cacacactga ctgtagttac cggaaatgga atagctgtag ataacaatgc cctccaaact 27660
agagttacgg gcgccctggg ttatgacaca tcaggaaacc tacaactgag agccgcgggg 27720
ggtatgcgaa ttgatgcaaa tggccaactt atccttgatg tggcataccc atttgatgct 27780
caaaacaatc tcagccttag acttggtcag ggacccctgt atgtaaacac agaccacaac 27840
ctagatttga attgcaacag aggtctgacc acaactacca ccaacaacac aaaaaaactt 27900
gaaactaaaa ttggctcagg cttagactat gataccaatg gtgctgtcat tattaaactt 27960
ggtactggtg taagctttga cagcacaggc gccctaactg tgggaaacac tggcgatgat 28020
aaactgactc tgtggacaac cccagaccca tctccaaatt gcagaattca cgcagacaaa 28080
gactgcaagt ttactctagt cctaactaag tgtggaagtc aaatcctggc ttctgtcgcc 28140
gccctagcgg tgtcaggaaa tctggcttca ataacaggca ccgttgccag cgttaccatc 28200
tttctcagat ttgatcagaa tggagtgctt atggaaaact cctccctaga caagcagtac 28260
tggaacttca gaaatggtaa ctcaaccaat gccaccccct acaccaatgc agttgggttc 28320
atgccaaacc tcgcagcata ccccaagaca cagagccaga ctgctaaaaa caacattgta 28380
agtcaggttt acttgaatgg ggacaaatcc aaacccatga cccttaccat taccctcaat 28440
ggaactaatg aatccagtga aactagccag gtgagtcact actccatgtc atttacatgg 28500
gcttgggaga gtgggcaata tgccaccgaa acctttgcca ccaattcctt taccttctct 28560
tacattgctg aacaataaag aaagcacaga gatgcttgtt tttgatttca aaattgtgtg 28620
cttttattta ttttcaagct tacagtattt ccagtagtca ttcgaataga gcttaatgaa 28680
actgcatgag aacccttcca catagcttaa attatcacca gtgcaaatgg agaaaaatca 28740
acataccttt ttatccagat atcacagaac cctagtattc aacctgccac ctccctccca 28800
acacacagag tacacagtcc tttctccccg gctggcctta aaaagcatca tatcatgggt 28860
aacagacata ttcttaggtg ttatattcca cacggtttcc tgtcgagcca aacgctcatc 28920
agtgatatta ataaactccc cgggcagctc acttaagttc atgtcgctgt ccagctgctg 28980
agccacaggc tgctgtccaa cttgcggttg cttaacgggc ggcgaaggag aagtccacgc 29040
ctacatgggg gtagagtcat aatcgtgcat caggataggg cggtggtgct gcagcagcgc 29100
gcgaataaac tgctgccgcc gccgctccgt cctgcaggaa tacaacatgg cagtggtctc 29160
ctcagcgatg attcgcaccg cccgcagcat aaggcgcctt gtcctccggg cacagcagcg 29220
caccctgatc tcacttaaat cagcacagta actgcagcac agcaccacaa tattgttcaa 29280
aatcccacag tgcaaggcgc tgtatccaaa gctcatggcg gggaccacag aacccacgtg 29340
gccatcatac cacaagcgca ggtagattaa gtggcgaccc ctcataaaca cgctggacat 29400
aaacattacc tcttttggca tgttgtaatt caccacctcc cggtaccata taaacctctg 29460
attaaacatg gcgccatcca ccaccatcct aaaccagctg gccaaaacct gcccgccggc 29520
tatacactgc agggaaccgg gactggaaca atgacagtgg agagcccagg actcgtaacc 29580
atggatcatc atgctcgtca tgatatcaat gttggcacaa cacaggcaca cgtgcataca 29640
cttcctcagg attacaagct cctcccgcgt tagaaccata tcccagggaa caacccattc 29700
ctgaatcagc gtaaatccca cactgcaggg aagacctcgc acgtaactca cgttgtgcat 29760
tgtcaaagtg ttacattcgg gcagcagcgg atgatcctcc agtatggtag cgcgggtttc 29820
tgtctcaaaa ggaggtagac gatccctact gtacggagtg cgccgagaca accgagatcg 29880
tgttggtcgt agtgtcatgc caaatggaac gccggacgta gtcatatttc ctccagcaga 29940
accaagtgcg cgcgtggcag ctatccttgc gtcttctgtc tcgccgcctg ccccgctcgg 30000
tgtagtagtt gtaatacagc cactccctca gaccgtcaag gcgctccctg gcgtccggat 30060
ctataacaac accatcctgc agcgccgccc tgatgacatc caccaccgta gagtatgcca 30120
agcccagcca ggaaatgcac tcactttgac agcgagagat aggaggagcg ggaagagatg 30180
gaagaaccat gatagtaaaa gaacttttat tccaatcgat cctctacaat gtcaaagtgt 30240
agatctatca gatggcactg gtctcctccg ctgagtcgat caaaaataac agctaaacca 30300
caaacaacac gattggtcaa atgctgcaca agggcttgca gcataaaatc gcctcgaaag 30360
tccaccgcaa gcataacatc aaagccaccg cccctatcat gatctatgat aaaaacccca 30420
cagctatcca ccagacccat atagttttca tctctccatc gtgaaaaaat atttacaagc 30480
tcctccttta aatcacctcc aaccaattca aaaagttgag ccagaccgcc ctccaccttc 30540
attttcagca tgcgcatcat gattgcaaaa attcaggctc ctcagacacc tgtataagat 30600
tgagaagcgg aacattaaca tcaatgtttc gctcgcgaag atcgcgcctc agtgcaagca 30660
tgatataatc ccacaggtcg gagcggatca gcgaggacat ctccccgcca ggaaccaact 30720
caacggagcc tatgctgatt ataatacgca tattcggggc tatgctaacc agcacggccc 30780
ccaaataggc gtactgcata ggcggcgaca aaaagtgaac agtttgggtt aaaaaatcag 30840
gcaaacactc gcgcaaaaaa gcaagaacat cataaccatg ctcatgcaaa tagatgcaag 30900
taagctcagg aacgaccaca gaaaaatgca caatttttct ctcaaacatg actgcgagcc 30960
ctgcaaaaaa taaaaaagaa acattacaca agagtagcct gtcttacaat gggatagact 31020
actctaacca acataagacg ggccacgaca tcgcccgcgt ggccataaaa aaaattatcc 31080
gtgtgattaa aaagaagcac agatagctgg ccagtcatat ccggagtcat cacgtgcgaa 31140
cccgtgtaga cccccgggtt ggacacatcg gccaaagaaa gaaagcggcc aatgtatccc 31200
ggaggaatga taacactaag acgaagatac aacagaataa ccccatgggg gggaataaca 31260
aagttagtag gtgaataaaa acgataaaca cccgaaactc cctcctgcgt aggcaaaata 31320
gcgccctccc cttccaaaac aacatacagc gcttccacag cagccatgac aaaagactca 31380
aaacactcaa aagactcagt cttaccagga aaataaaagc actctcacag caccagcact 31440
aatcagagtg tgaagagggc caagtgccga acgagtatat ataggaatta aaaatgacgt 31500
aaatgtgtaa aggtcaaaaa acgcccagaa aaatacacag accaacgccc gaaacgaaaa 31560
cccgcgaaaa aatacccaga agttcctcaa caaccgccac ttccgctttc ccacgatacg 31620
tcacttcctc gaaaatagca aactacattt cccacatgta caaaaccgaa accactcccc 31680
ttgtcaccgc ccacaactta catcttaatt aacaaacgtc aaagcctacg tcagccgccc 31740
cgcctcgccc cgcccacctc attatcatat tggccacaat ccaaaataag gtatattatt 31800
gatgatg 31807
<210> 12
<211> 31656
<212> DNA
<213> 人工序列
<220>
<223> BZ1/BZ28.5IXP腺病毒载体
<400> 12
catcatcaat aatatacctt attttggatt gtggccaata tgataatgag gtgggcgggg 60
cgaggcgggg cgggtgacgt aggacgtgcg agtagggttg ggaggtgtgg cggaagtgtg 120
gcatttgtaa gtgggaggag cttacatgca agcttccgtc gcggaaaatg tgacgttttt 180
tatgagcgcc gcctacctcc ggaagtgcca attttcgcgc gctttttacc ggatatcgta 240
gtaattttgg gcgggaccat gtaagatttg gccattttcg cgcgaaaagt gaaacgggga 300
agtgaaaact gaataatagg gcgttagtca tagcgcgtaa tatttaccga gggccgaggg 360
actttgaccg attacgtgga ggactcgccc aggtgttttt tacgtgaatt tccgcgttcc 420
gggtcaaagt ctccgttttt attgtcaccg tcatttgacg cggagggtat ttaaacccgc 480
tgcgctccta gcgatcgcgg tactgaaatg tgtgggcgtg gcttaagggt gggaaagaat 540
atataaggtg ggggtcttat gtagttttgt atctgttttg cagcagccgc cgccgccatg 600
agcgacaccg gcaacagctt tgatggaagc atctttagcc cctatctgac agtgcgcatg 660
cctcactggg ccggagtgcg tcagaatgtg atgggttcca acgtggatgg acgtcccgtt 720
ctgccttcaa attcgtctac gatggcctac gcgaccgtgg gaggaactcc gctggacgcc 780
gcgacctccg ccgccgcctc cgccgccgcc gcgaccgcgc gcagcatggc tacggacctt 840
tacagctctt tggtggcgag cagcgcggcc tctcgcgcgt ctgctcggga tgaaaaactg 900
actgctctgc tgcttaaact ggaagacttg acccgggagc tgggtcaact gacccagcag 960
gtctccagct tgcgtgagag cagccttgcc tccccctaat ggcccataat ataaataaaa 1020
gccagtctgt ttggattaag caagtgtatg ttctttattt aactctccgc gcgcggtaag 1080
cccgggacca gcggtctcgg tcgtttaggg tgcggtggat tctttccaac acgtggtaca 1140
ggtggctctg gatgtttaga tacatgggca tgagtccatc cctggggtgg aggtagcacc 1200
actgcagagc ttcgtgctcg ggggtggtgt tgtatatgat ccagtcgtag caggagcgct 1260
gggcgtggtg ctgaaaaatg tccttaagca agaggcttat agctaggggg aggcccttgg 1320
tgtaagtgtt tacaaatctg cttagctggg aggggtgcat ccggggggat atgatgtgca 1380
tcttggactg gatttttagg ttggctatgt tcccacccag atcccttctg ggattcatgt 1440
tgtgcaggac caccagcacg gtatatccag tgcacttggg aaatttatcg tggagcttag 1500
acgggaatgc atggaagaac ttggagacgc ccttgtggcc tcccagattt tccatacatt 1560
cgtccatgat gatggcaatg ggcccgtggg aagctgcctg agcaaaaacg tttctggcat 1620
cgctcacatc gtagttatgt tccagggtga ggtcatcata ggacatcttt acaaatcggg 1680
ggcggagggt cccggactgg gggatgatgg taccctcggg ccccggggcg tagttcccct 1740
cacagatctg catctcccag gctttcattt cagagggagg gatcatatcc acctgcgggg 1800
cgatgaaaaa gacagtttct ggcgcagggg agattaactg ggatgagagc aggtttctga 1860
gcagctgtga ctttccacag ccggtgggcc catatatcac gcctatcacc ggctgcagct 1920
ggtagttaag agagctgcag ctgccgtcct cccggagcag gggggccacc tcgttgagca 1980
tatccctgac gtggatgttc tccctgacca gttccgccag aaggcgctcg ccgcccagcg 2040
aaagcagctc ttgcaaggaa gcaaaatttt tcagcggttt caggccatcg gccgtgggca 2100
tgtttttcag cgtctgggtc agcagctcca gcctgtccca gagctcggtg atgtgctcta 2160
cggcatctcg atccagcaga tctcctcgtt tcgcgggttg gggcggcttt cgctgtaggg 2220
caccagccga tgggcgtcca gcggggccag agtcatgtcc ttccatgggc gcagagtcct 2280
cgtcagggtg gtctgggtca cggtgaaggg gtgcgctccg ggttgggcac tggccagggt 2340
gcgcttgagg ctggttctgc tggtgctgaa tcgctgccgc tcttcgccct gcgcgtcggc 2400
caggtagcat ttgaccatgg tctcgtagtc gagaccctcg gcggcgtgcc ccttggcgcg 2460
gagctttccc ttggaggtgg cgccgcacga ggggcactgc aggctcttca gggcgtagag 2520
cttgggagcg agaaacacgg actctgggga gtaggcgtcc gcgccgcagg ccgcgcagac 2580
cgtctcgcat tccaccagcc aagtgagttc cgggcggtca gggtcaaaaa ccaggttgcc 2640
cccatgcttt ttgatgcgtt tcttacctcg gctctccatg aggcggtgtc ccttctcggt 2700
gacgaagagg ctgtccgtgt ccccgtagac cgacttcagg ggcctgtctt ccagcggagt 2760
gcctctgtcc tcctcgtaga gaaactctga ccactctgag acgaaggccc gtgtccaggc 2820
caggacgaag gaggccacgt gggaggggta gcggtcgttg tccactagcg ggtccacctt 2880
ctccagggtg tgcagacaca tgtccccctc ctccgcgtcc agaaaagtga ttggcttata 2940
ggtgtaggac acgtgaccgg gggttcccga cgggggggta taaaaggggg tgggcgccct 3000
ttcatcttca ctctcttccg catcgctgtc tgcgagagcc agctgctggg gtaagtattc 3060
cctttcaaag gcgggcatga cctcagcgct caggttgtca gtttctaaaa atgaggagga 3120
tttgatgttc acctgtccgg aagtgatacc tttgagggta cctgggtcca tctggtcaga 3180
aaacactatt tttttgttgt caagcttggt ggcgaacgac ccgtagaggg cgttggagag 3240
cagcttggca atggagcgca gggtctggtt tttgtcgcgg tcggctcgct ccttggccgc 3300
gatgttgagt tgcacgtatt cgcgggccac gcacttccac tcggggaaga cggtggtgcg 3360
ctcgtctggg atcaggcgca ccctccagcc gcggttgtgc agggtgacca tgtcgacgct 3420
ggtggcgacc tcaccgcgca gacgctcgtt ggtccagcag aggcggccgc ctttgcgcga 3480
gcagaagggg ggtagggggt ccagctggtc ctcgtttggg gggtccgcgt cgatggtaaa 3540
gaccccgggg agcaggcgcg ggtcaaagta gtcgatcttg caagcttgca tgtccagagc 3600
ccgctgccat tcgcgggcgg cgagcgcgcg ctcgtagggg ttgaggggcg ggccccaggg 3660
catggggtgg gtgagcgcgg aggcgtacat gccgcagatg tcatacacgt acaggggttc 3720
cctgaggata ccgaggtagg tggggtagca gcgccccccg cggatgctgg cgcgcacgta 3780
gtcatagagc tcgtgggagg gggccagcat gttgagccca aggttggtgc gctgggggcg 3840
ctcggcgcgg aagacgatct gtctgaagat ggcatgggag ttggaggaga tggtgggtcg 3900
ctggaagacg ttgaagcttg cttcttgcaa gcccacggag tccctgacga aggaggcgta 3960
ggactcgcgc agcttgtgca ccagctcggc ggtgacctgg acgtcgagcg cgcagtagtc 4020
gagggtctcg cggatgatgt catacttatc ctcccccttc tttttccaca gctcgcggtt 4080
gaggacgaac tcttcgcggt ctttccagta ctcttggagg ggaaacccgt ccgtgtccga 4140
acggtaagag cctagcatgt agaactggtt gacggcctgg taggggcagc agcccttctc 4200
cacgggcagc gcgtaggcct gcgccgcctt gcggagggag gtgtgggtaa gggcgaaagt 4260
gtccctgacc atgactttga ggtattgatg tctgaagtct gtgtcatcgc agccgccctg 4320
ttcccacagg gtgtagtccg tgcgcttttt ggagcgcggg ttgggcaggg agaaggtgag 4380
gtcattgaag aggatcttcc ccgctcgagg catgaagttt ctggtgatgc gaaagggccc 4440
tgggaccgag gagcggttgt tgatgacctg ggcggccagg acgatctcgt caaagccgtt 4500
tatgttgtgg cccacgatgt agagctccag gaagcggggc tggcccttga tggaggggag 4560
ctttttaagt tcctcgtagg taagctcctc gggcgattcc aggccgtgct cctccagggc 4620
ccagtcttgc aagtgagggt tggccgccag gaaggatcgc cagaggtcgc gggccatgag 4680
ggtctgcagg cggtcgcgga aggttctgaa ctgccgccct acggccatct tttcgggggt 4740
gatgcagtag aaggtgaggg ggtctttctc ccaggggtcc catctgagct cttgggcgag 4800
gtcgcgcgcg gcggcgacca gagcctcgtc gccccccagt ttcatgacca gcatgaaggg 4860
cacgagctgc ttgccaaagg ctcccatcca agtgtaggtc tctacatcgt aggtgacaaa 4920
gaggcgctcc gtgcgaggat gagagccgat cgggaagaac tggatctccc gccaccagtt 4980
ggaggattgg ctgttgatgt ggtgaaagta gaagtcccgt ctgcgggccg agcactcgtg 5040
ctggcttttg taaaagcgac cgcagtactg gcagcgctgc acgggttgta tatcttgcac 5100
gaggtgaacc tggcgacctc tgacgaggaa gcgcagcggg aatctaagtc ccccgcctgg 5160
ggtcccgtgt ggctggtggt cttctacttt ggttgtctgg ccgccagcat ctgtctcctg 5220
gagggcgatg gtggagcaga ccaccacgcc gcgagagccg caggtccaga tctcggcgct 5280
cggcgggcgg agtttgatga cgacatcgcg cacattggag ctgtccatgg tctccagctc 5340
ccgcggcggc aggtcagccg ggagttcctg gaggttcacc tcgcagagac gggtcaaggc 5400
gcggacagtg ttgagatggt atctgatttc aaggggcgtg ttggaggcgg agtcgatggc 5460
ttgcagaagg ccgcagcccc ggggggccac gatggttccc cgcggggcgc gaggggaggc 5520
ggaagctggg ggtgtgttca gaagcggtga cgcgggcggg cccccggagg tagggggggt 5580
tccggcccca caggcatggg cggcaggggc acgtcttcgc cgcgcgcggg caggggctgg 5640
tgctggctcc ggagagcgct tgcgtgcgcg acgacgcgac ggttggtgtc ctgtatctgg 5700
cgcctctgag tgaagaccac gggtcccgtg accttgaacc tgaaagagag ttcgacagaa 5760
tcaatctcgg catcgttgac agcggcctgg cgcaggatct cctgcacgtc gcccgagttg 5820
tcctggtagg cgatctctgc catgaactgc tcgatctctt cctcctggag atctcctcgt 5880
ccggcgcgct ccacggtggc cgccaggtcg ttggagatgc gacccatgag ctgcgagaag 5940
gcgttgaggc cgccctcgtt ccagacccgg ctgtagacca cgcccccctc ggcgtcgcgg 6000
gcgcgcatga ccacctgggc caggttgagc tccacgtgtc gcgtgaagac ggcgtagttg 6060
cgcaggcgct ggaaaaggta gttcagggtg gtggcggtgt gttcggcgac gaagaagtac 6120
atgacccagc gccgcaacgt ggattcattg atgtccccca aggcctccag gcgctccatg 6180
gcctcgtaga agtccacggc gaagttgaaa aactgggagt tgcgagcgga cacggtcaac 6240
tcctcctcca gaagacggat gagctcggcg acagtgtcgc gcacctcgcg ctcgaaggcc 6300
acggggggcg cttcttcctc ttccacctct tcttccatga ttgcttcttc ttcctcagcc 6360
gggacgggag ggggcggcgg cgggggaggg gcgcggcggc ggcggcggcg caccggcagg 6420
cggtcgatga agcgctcgat catctccccc cgcatgcggc gcatggtctc ggtgacggcg 6480
cggccgttct cccgggggcg cagctcaaag acgccgcctc tcatctcgcc gcggggcggg 6540
cggccgtgag gtagcgagac ggcgctgact atgcatctta acaattgctg tgtaggtacg 6600
ccgccaaggg acctgattga gtccagatcc accggatccg aaaacctttg gaggaaagcg 6660
tctatccagt cgcagtcgca aggtaggctg agcaccgtgg cgggcggggg cgggtcggga 6720
gagttcctgg cggagatgct gctgatgatg taattaaagt aggcggtctt gagaaggcgg 6780
atggtggaca ggagcaccat gtctttgggt ccggcctgtt ggatgcggag gcggtcggcc 6840
atgccccagg cctcgttctg acaccggcgc aggtctttgt agtagtcttg catgagtctt 6900
tccaccggca cctcttctcc ttcctcttct ccatctcgcc ggtggtttct cgcgccgccc 6960
atgcgcgtga ccccaaagcc cctgagcggc tgcagcaggg ccaggtcggc gaccacgcgc 7020
tcggccaaga tggcctgctg tacctgagtg agggtcctct cgaagtcatc catgtccacg 7080
aagcggtggt aggcgcccgt gttgatggtg taggtgcagt tggccatgac ggaccagttg 7140
acggtctggt gtcccggctg cgagagctcc gtgtaccgca ggcgcgagaa ggcgcgggaa 7200
tcgaacacgt agtcgttgca agtccgcacc agatactggt agcccaccag gaagtgcggc 7260
ggaggttggc gatagagggg ccagcgctgg gtggcggggg cgccgggcgc caggtcttcc 7320
agcatgaggc ggtggtatcc gtagatgtac ctggacatcc aggtgatgcc ggcggcggtg 7380
gtggtggcgc gcgcgtagtc gcggacccgg ttccagatgt ttcgcagggg cgagaagtgt 7440
tccatggtcg gcacgctctg gccggtgagg cgcgcgcagt cgttgacgct ctatacacac 7500
acaaaaacga aagcgtttac agggctttcg ttctgtagcc tggaggaaag taaatgggtt 7560
gggttgcggt gtgccccggt tcgagaccaa gctgagctcg gccggctgaa gccgcagcta 7620
acgtggtatt ggcagtcccg tctcgaccca ggccctgtat cctccaggat acggtcgaga 7680
gcccttttgc tttcttggcc aagcgcccgt ggcgcgatct gggatagatg gtcgcgatga 7740
gaggacaaaa gcggctcgct tccgtagtct ggagaaacaa tcgccagggt tgcgttgcgg 7800
cgtaccccgg ttcgagcccc tatggcggct tggatcggcc ggaaccgcgg ctaacgtggg 7860
ctgtggcagc cccgtcctca ggaccccgcc agccgacttc tccagttacg ggagcgagcc 7920
ccttttgttt tttatttttt agatgcatcc cgtgctgcgg cagatgcgcc cctcgccccg 7980
gcccgatcag cagcagcaac agcaggcatg cagacccccc tctcctctac ccgccccggt 8040
caccacggcc gcggcggccg tgtccggcgc ggggggcgcg ctggagtcag atgagccacc 8100
gcggcggcga cctaggcagt atctggactt ggaagagggc gagggactgg cgcggctggg 8160
ggcgagctct ccagagcgcc acccgcgggt gcagttgaaa agggacgcgc gcgaggcgta 8220
cctgccgcgg cagaacctgt ttcgcgaccg cgggggcgag gagcccgagg agatgcgaga 8280
ctgcaggttc caagcggggc gcgagctgcg ccgcgggttg gacagacagc gcctgctgcg 8340
cgaggaggac tttgagcccg acacgcagac gggcatcagc cccgcgcgcg cgcacgtggc 8400
cgcggccgac ctggtgaccg cctacgagca gacggtgaac caggagcgca acttccaaaa 8460
aagcttcaac aaccacgtgc gcacgctggt ggcgcgcgag gaggtgaccc tgggtctcat 8520
gcatctgtgg gacctggtgg aggcgatcgt gcagaacccc agcagcaagc ccctgaccgc 8580
gcagctgttc ctggtggtgc agcacagcag ggacaacgag gccttcaggg aggcgctgct 8640
gaacatcacc gagccggagg ggcgctggct cctggacctg ataaacatcc tgcagagcat 8700
agtggtgcag gagcgcagcc tgagcctggc cgagaaggtg gcggccatta actattctat 8760
gctgagcttg ggcaagttct acgcccgcaa gatctacaag accccctacg tgcccataga 8820
caaggaggtg aagatagaca gcttctacat gcgcatggcg ctaaaggtgc tgaccctgag 8880
cgacgacctg ggagtgtacc gcaacgagcg catccacaag gccgtgagcg ccagccggcg 8940
gcgcgagctg agcgaccgcg agctgatgca cagtctgcag cgcgcgctca ccggcgcggg 9000
cgagggcgac agggaggtcg agtcctactt cgacatgggg gctgacctgc actggcagcc 9060
gagccgccgc gccctggagg cggcgggggc gtatggcggc cccctggcgg ccgatgacga 9120
ggaagaggag gactatgagc tagaggaggg cgagtacctg gaggactgac ctggctggtg 9180
gtgttttggt atagatgcaa gatccgaacg tggcggaccc ggcggtccgg gcggcgctgc 9240
agagccagcc gtccggcatt aactcctctg acgactgggc cgcggccatg ggtcgcatca 9300
tggccctgac cgcgcgcaac cccgaggcct tcaggcagca gcctcaggct aaccggctgg 9360
cggccatctt ggaagcggta gtgcccgcgc gctccaaccc cacccacgag aaggtgctgg 9420
ccatagtcaa cgcgctggcg gagagcaggg ccatccgggc ggacgaggcc ggactggtgt 9480
acgatgcgct gctgcagcgg gtggcgcggt acaacagcgg caacgtgcag accaacctgg 9540
accgcctggt gacggacgtg cgcgaggccg tggcgcagcg cgagcgcttg catcaggacg 9600
gtaacctggg ctcgctggtg gcgctaaacg ccttcctcag cacccagccg gccaacgtac 9660
cgcgggggca ggaggactac accaacttct tgagcgcgct gcggctgatg gtgaccgagg 9720
tccctcagag cgaggtgtac cagtcggggc ccgactactt cttccagacc agcagacagg 9780
gcttgcaaac cgtgaacctg agccaggctt tcaagaacct gcgggggctg tggggagtga 9840
aagcgcccac cggcgaccga gctacggtgt ccagcctgct aacccccaac tcgcgcctgc 9900
tgctgctgct gatcgcgccc ttcacggaca gcgggagcgt ctcgcgggag acctatctgg 9960
gccacctgct gacgctgtac cgcgaggcca tcgggcaggc gcaggtggac gagcacacct 10020
tccaagagat caccagcgtg agccacgcgc tggggcagga ggacacgggc agcctgcagg 10080
cgaccctgaa ctacctgctg accaacaggc ggcagaagat tcccacgctg cacagcctga 10140
cccaggagga ggagcgcatc ttgcgctacg tgcagcagag cgtgagcctg aacctgatgc 10200
gcgacggcgt gacgcccagc gtggcgctgg acatgaccgc gcgcaacatg gaaccgggca 10260
tgtacgcttc ccagcggccg ttcatcaacc gcctgatgga ctacttgcat cgggcggcgg 10320
ccgtgaaccc cgagtacttc accaatgcca ttctgaatcc ccactggatg ccccctccgg 10380
gtttctacaa cggagacttc gaggtgcctg aggtcaatga tgggttcctc tgggatgaca 10440
tggatgacag tgtgttctcc cccaacccgc tgcgcgccgc gtctctgcga ttgaaggagg 10500
gctctgacag ggaaggaccg aggagtctgg cctcctccct ggctctgggg gcggtgggcg 10560
ccacgggcgc ggcggcgcgg ggcagcagcc ccttccccag cctggcagac tctctgaata 10620
gcgggcgggt gagcaggccc cgcttgctag gcgaggagga gtatctgaac aactccctgc 10680
tgcagcccgt gagggacaaa aacgctcagc gacagcagtt tcccaacaac gggatagaga 10740
gcctggtgga caagatgtcc agatggaaga catatgcgca ggagtacaag gagtgggagg 10800
accgccagcc gcggcccctg ccgcccccta gacagcgctg gcagcggcgc gcgtccaacc 10860
gccgctggag acagggaccc gaggacgatg atgactctgc agatgacagc agcgtgttgg 10920
acctgggcgg gagcgggaac cccttttcgc acctgcgccc acgcctgggc aagatgtttt 10980
aaaagagaaa aataaaactc accaaggcca tggcgacgag cgttggtttt ttgttccctt 11040
ccttagtatg cggcgcgcgg cgatgttcga ggaggggcct cccccctctt acgagagcgc 11100
gatgggaatt tctcctgcgg cgcccctgca gcctccctac gtgcctcctc ggtacctgca 11160
acctacaggg gggagaaata gcatctgtta ctctgagctg cagcccctgt acgataccac 11220
cagactgtac ctggtggaca acaagtccgc ggacgtggcc tccctgaact accagaacga 11280
ccacagcgat tttttgacca cggtgatcca aaacaacgac ttcaccccaa ccgaggccag 11340
tacccagacc ataaacctgg acaacaggtc gaactggggc ggcgacctga agaccatcct 11400
gcacaccaac atgcccaacg tgaacgagtt catgtttacc aactctttta aggcgcgggt 11460
tatggtggcg cgcgagcagg gggaggcgaa gtacgagtgg gtggacttca cgctgcccga 11520
gggcaactac tcagagacca tgactattga cctgatgaac aatgcgatcg tggaacacta 11580
cctgaaagtg ggcaggcaga acggggtgaa ggagagcgat atcggggtca agtttgacac 11640
cagaaacttt cgtctgggct gggaccccgt gaccgggctg gtcatgccgg gggtctacac 11700
caacgaggcc tttcatcccg atatagtgct cctgcccggc tgtggggtgg actttaccca 11760
gagccggctg agcaacctgc tgggcgttcg caagcggcaa cctttccagg agggtttcaa 11820
gatcacctat gaggatctgg aggggggcaa cattcccgcg ctccttgatc tggacgccta 11880
cgaggagagc ttgaaacccg aggagagcgc tggcgacagc ggcgagagtg gcgaggagca 11940
agccggcggc ggtggcagcg cgtcggtaga aaacgaaagt actcccgcag tggcggcaga 12000
cgctgcggag gtcgagccag aggccatgca gcaggacgca gaggagggcg cacaggaggg 12060
cgcgcaggag gacatgaacg atggggagat caggggagac actttcgcca cccggggcga 12120
agaaaaagag gcagaggcgg tggcggcgac ggtggaagcc gaaaccgagg cagaggcaga 12180
gcccaagacc gaagttatgg aagacatgaa tgatggagaa cgtaggggtg acacgtttgc 12240
cacccggggc gaagagaagg cggcggaggc agaagccgcg gctgaggagg cggctgcggc 12300
tgcggccgag gctgaggctg cggctgaggc taaggtcgaa gccgatgttg cggttgaggc 12360
tcaggctgag gaggaggagg cggcgactga agcagttaag gaaaaggccc aggcagagca 12420
ggaagagaaa aaacctgtca ttcaacctct aaaagaagat agcaaaaagc gcagttacaa 12480
cgtcatcgag ggcagcacct ttacccagta ccgcagctgg tacctggcgt acaactacgg 12540
cgacccggtc aagggggtgc gctcgtggac cctgctctgc acgccggacg tcacctgcgg 12600
ctccgagcag atgtattggt cgctgccaaa catgatgcaa gacccggtga ccttccgctc 12660
cacgcggcag gttagcaact tcccggtggt gggcgccgaa ctgctgcccg tgcactccaa 12720
gagtttttac aacgagcagg ccgtctactc ccagctgatc cgccaggcca cctctctgac 12780
ccacgtgttc aatcgctttc ccgagaacca gattttggcg cgcccgccgg cccccaccat 12840
caccaccgtc agtgaaaacg ttcctgccct cacagatcac gggacgctac cgctgcgcaa 12900
cagcatctca ggagtccagc gagtgaccat tactgacgcc agacgccgga cctgccccta 12960
cgtttacaag gccttgggca tagtctcgcc gcgcgtcctc tccagtcgca ctttttaaaa 13020
tacatctacc ctcacgcttc aaaatcatgt ccgtactcat ctcacccagc aacaacaccg 13080
gctgggggct gcgcgcgccc agcaagatgt ttggaggggc gaggaaacgc tccgagcagc 13140
acccagtgcg cgtgcgcggc cactaccgcg cgccctgggg agcgcacaag cgcgggcgcg 13200
cagggcgcac cactgtggac gacgccattg actccgtagt ggagcaggcg cgccactaca 13260
cacccggcgc gccgtccgcc cccgccgtgt ccaccgtgga cgaggcgatc gagagcgtgg 13320
tacagggcgc gcggcactat gccaacctta aaaatcgacg ccgtcgcgtg gctcgccgcc 13380
atcgccggag accccgggcc accgccgccg cgcgccttgc taaggctctg ctcaggcgcg 13440
ccaggcgaac tggccgccgg gccgccatga gggccgcacg gcgggctgcc gccagcgcgg 13500
ccgccgcggc cccacgggca cgaaggcgcg cggccgctgc cgccgccgcc gccatttcca 13560
gcttggcctc gacgcggcgc ggtaacatat actgggtgcg cgactcggta accggcacgc 13620
gggtacccgt gcgctttcgc cccccgcgga attagcacaa gacaacatac acactgagtc 13680
tcctgctgtt gtgtatccca gcggcgaccg tcagcagcgg cgacatgtcc aagcgcaaaa 13740
ttaaagaaga gatgctccag gtcatcgcgc cggagatcta tgggcccccg aagaaggagg 13800
aggatgatta caagccccgc aagctaaagc gggtcaaaaa gaaaaagaaa gatgatgacg 13860
ttgacgaggc ggtggagttt gtccgccgca tggcgcccag gcgccccgtg cagtggaagg 13920
gtcggcgcgt gcagcgagtc ctgcgccccg gcaccgcggt ggtctttacg cccggcgagc 13980
gttccacgcg cactttcaag cgggtgtacg atgaggtgta cggcgacgag gatctgttgg 14040
agcaggccaa ccatcgcttt ggggagtttg catatgggaa acggccccgc gagagcctaa 14100
aagaggacct gctggcgcta ccgctggacg agggcaatcc caccccgagt ctgaagccgg 14160
taaccctgca acaggtgctg cctttgagcg cgcccagcga gcagaagcga gggttgaagc 14220
gcgagggcgg ggacctggca cccaccgtgc agttgatggt gcccaagcgg cagaagctgg 14280
aggacgtgct ggagaaaatg aaagtagagc ccgggatcca gcccgaaatc aaggtccgcc 14340
ccatcaagca ggtggcgccc ggcgtgggag tccagaccgt ggacgttagg attcccacgg 14400
aggagatgga aacccaaacc gccactccct cttcggcggc tagcgccacc accggctccg 14460
cttcggtaga ggtgcagacg gacccctggc tagccgccgc cgccccggcc gccccccgtt 14520
cgcgcgggcg caagagaaat tatccagcgg ccagcgcgct catgccccag tacgcactgc 14580
atccatccat cgcgcccacc cccggctacc gcgggtactc gtaccgcccg cgcagatcag 14640
ccggcacccg cggccgccgc cgccgtgcga ccacaaccag ccgccgccgt cgccgccgcc 14700
gccagccagt gctgaccccc gtgtctgtaa ggaaggtggc tcgctcgggg agcacgctgg 14760
tggtgcccag agcgcgctac caccccagca ttgtttaaag ccggtctctg tatggttctt 14820
gcagatatgg ccctcacttg tcgcctccgc ttcccggtgc cgggataccg aggaagaact 14880
caccgccgca gaggcatggc gggcagtggt ctccgcggcg gccgtcgcca tcgccggcgc 14940
gcaaagagca ggcgcatgcg cggcggtgtg ctgcccttcc taatcccgct aatcgccgcg 15000
gcgatcggtg ccgtgcccgg gatcgcctcc gtggccctgc aggcgtccca gaaacattga 15060
ctcttgcaac cttgcaagct tgcatttttt ggaggaaaaa ataaaaagtc tagactctca 15120
cgctcgcttg gtcctgtgac tattttgtag aaaaaagatg gaagacatca actttgcgtc 15180
gctggccccg cgtcacggct cgcgcccgtt catgggagac tggacagata tcggcaccag 15240
caatatgagc ggtggcgcct tcagctgggg cagtctgtgg agtggcctta aaaattttgg 15300
ttccaccatt aagaactatg gcaacaaagc gtggaacagc agcacgggtc agatgctgag 15360
agacaagttg aaagagcaga acttccagga gaaggtggca cagggcctgg cctctggcat 15420
cagcggggtg gtggacatag ctaaccaggc cgtgcagaaa aagataaaca gtcatctgga 15480
cccccgccct caggtggagg aaacgcctcc agccatggag acggtgtctc ccgagggcaa 15540
aggcgaaaag cgcccgcggc ccgacaggga agagaccctg gtgtcacaca ccgaggagcc 15600
gccctcttac gaggaggcag tcaaggccgg cctgcccacc actcgcccca tagctcccat 15660
ggccaccggt gtggtgggtc acaggcaaca cacccccgcg acactagatc tgcccccgcc 15720
gtccgagccg actcgccagc caaaggcggt gacggtgccc gctccctcca cttccgccgc 15780
caacagagtg cctctgcgcc gcgctgcgag cggcccccgg gcctcgcgag tcagcggcaa 15840
ctggcagagc acactgaaca gcatcgtggg cctgggagtg aggagtgtga agcgccgccg 15900
ttgctactga atgagcaagc tagctaacgt gttgtatgtg tgtatgcgtc ctatgtcgcc 15960
gccagaggag ctgttgagcc gccggcgccg tctgcactcc agcgaatttc aagatggcga 16020
ccccatcgat gatgcctcag tggtcgtaca tgcacatctc gggccaggac gcttcggagt 16080
acctgagccc cgggctggtg cagttcgccc gcgccacaga cacctacttc aacatgagta 16140
acaagttcag gaaccccact gtggcgccca cccacgatgt gaccacggac cggtcgcagc 16200
gcctgacgct gcggttcatc cccgtggatc gggaggacac cgcttactct tacaaggcgc 16260
ggttcacgct ggccgtgggc gacaaccgcg tgctggacat ggcctccact tactttgaca 16320
tccggggggt gctggacagg ggccccactt tcaagcccta ctcgggcact gcctacaact 16380
ccctggcccc caagggcgct cccaattctt gcgagtggga ggaggaaaca caaaatgagg 16440
tacaagccaa tgaagaacaa ctagcagaag aagaggatga agaaatggct caagaggatc 16500
agcagcctac taaaaaaacc catgtatatg ctcaggcacc tctttctggc gaacagatta 16560
ccaaagatgg cttgcaaata ggagctgaag ttacaggaga aacatcaaag cccatttttg 16620
cagacaagac attccaacca gaacctcaga taggagagtc tcaatggaat gaggccgatg 16680
ctacagtagc aggaggtagg gttttgaaaa agactacccc tatgaaacct tgctatggat 16740
cctatgccag acctaccaat gccaatggag ggcaggggat acttgaggca aatgctaaag 16800
gggaactcga atctaaagtt gagatgcagt ttttctctaa caccacaact cttaatgtaa 16860
gagacggtga aaatggcctt aaaccaaaag tagtgctgta tagcgaagat gtcaacctgg 16920
aatcccctga cactcatctg tcttacaagc ccaaaaaaga tgatgttaat gccaaaatca 16980
tgttgggtca gcaagccatg cccaacagac ccaacctcat tggatttaga gataatttca 17040
ttgggctcat gtattacaac agcactggaa acatgggagt gctggcgggt caggcctctc 17100
agttgaatgc tgtggtggac ttgcaggata gaaacacgga actgtcatat cagcttatgc 17160
ttgattccat tggagataga accagatact tttccatgtg gaaccaggca gtggatagct 17220
atgacccaga tgttagaatc attgaaaacc atggggtgga ggatgagctg cccaactact 17280
gttttccctt gggcggtata ggaattacag atacatacca ggccataaaa gcagccaatg 17340
gtggagatgc tactacgtgg tctgctgata acacatttgc agaccgcaac gaaatagggg 17400
tgggaaacaa cttcgccatg gagatcaaca tccaggccaa cctctggaga aacttcctct 17460
atgcgaacgt gggactctac ctgccagaca agctcaagta caaccccacc aacgtggaca 17520
tctctgacaa ccccaacacc tatgactaca tgaacaagcg ggtggtggcc cccggcctgg 17580
tggactgctt tgtcaatgtg ggagccaggt ggtccctgga ctacatggac aacgtcaacc 17640
ccttcaacca ccaccgcaat gcgggtctgc gctaccgctc catgatcctg ggcaacgggc 17700
gctatgtgcc cttccacatc caggtacccc agaagttctt tgccatcaag aacctcctgc 17760
tcctgcccgg ctcctacacc tacgagtgga acttcaggaa ggatgtaaac atggtcctac 17820
agagctctct gggcaatgac cttagggtag atggggccag catcaagttt gacagcatca 17880
ccctctatgc tacatttttc cccatggccc acaacaccgc ctccacgctt gaggccatgc 17940
tgagaaacga caccaacgac cagtccttca atgactacct ctctggggcc aacatgctct 18000
acccaatccc agccaaggcc accaacgtgc ccatctccat cccctctcgc aactgggccg 18060
cctttagagg ctgggccttt acccgcctta agaccaagga gaccccctcc ctgggctcgg 18120
gttttgatcc ctactttgtt tactcgggat ccatccccta cctggatggc accttctacc 18180
tcaaccacac tttcaagaag atatccatca tgtatgactc ctccgtcagc tggccgggca 18240
acgaccgctt gctcaccccc aatgagttcg aggtcaagcg cgccgtggac ggcgagggct 18300
acaacgtggc ccagtgcaac atgaccaagg actggttcct ggtgcagatg ctggccaact 18360
acaacatagg ctaccagggc ttctacatcc cagagagcta caaggacagg atgtattcct 18420
tcttcagaaa tttccaaccc atgagccgac aggtggtgga cgagaccaat tacaaggact 18480
atcaggccat tggcatcacc caccagcaca acaactcggg tttcgtgggc tacctggcgc 18540
ccaccatgcg cgagggacag gcctaccccg ccaacttccc ctaccccctg ataggcaaga 18600
ccgcggtcga cagcgtcacc cagaaaaagt tcctctgcga ccgcaccctc tggcgcatcc 18660
ccttctctag caactttatg tccatgggtg cgctcacgga cctgggccaa aacctgcttt 18720
atgccaactc tgcccatgcg ctggacatga cttttgaggt ggaccccatg gacgagccca 18780
cccttctcta tattgtgttt gaagtgttcg acgtggtcag agtgcaccag ccgcaccgcg 18840
gtgtcatcga gaccgtgtac ctgcgtacgc ccttctcagc cggcaacgcc accacctaag 18900
gagacagcgc cgccgcctgc atgactggtt ccaccgagca agagctcagg gccatcgcca 18960
gagacctggg atgcggaccc tattttttgg gcacctatga caaacgcttc ccgggtttta 19020
tctcccgaga caagctcgcc tgcgccatcg tcaacacggc cgcgcgcgag accgggggcg 19080
tgcactggct ggcctttggc tgggacccgc gctctaaaac ttgctacctc tttgacccct 19140
ttggcttctc cgatcagcgc ctcaggcaga tttatgagtt tgagtacgag gggctgttgc 19200
gccgcagcgc gcttgcctcc tcgcccgacc gctgcatcac ccttgagaag tccaccgaga 19260
ccgtgcaggg gccccactcg gccgcctgcg gtctcttctg ttgcatgttt ttgcacgcct 19320
ttgtgcactg gcctcagagt cccatggatc gcaaccccac catgaacttg ctaaagggag 19380
tgcccaacgc catgctccag agcccccagg tcctgcccac cctgcgccgc aaccaggaac 19440
agctctaccg cttcctggag cgccactccc cctacttccg cagccacagc gcgcgcatcc 19500
ggggggccac ctctttttgc cacttgcaag aaaacatgca agacggaaaa tgatgtacag 19560
catgctttta ataaatgtaa agactgtgca ctttatttat acacgggctc tttctggtta 19620
tttattcaac accgccgtcg ccatctagaa atcgaaaggg ttctgccgcg cgtcgccgtg 19680
cgccacgggc agagacacgt tgcgatactg gaagcggctc gcccacttga actcgggcac 19740
caccatgcgg ggcagtggtt cctcggggaa attctcgctc cacagggtgc gggtcagctg 19800
cagcgcgctc aggaggtcgg gagccgagat cttgaagtcg cagttggggc cggaaccctg 19860
cgcgcgcgag ttgcggtaca cggggttgca gcactggaac accagcaggg ccggattatt 19920
cacgctggcc agcaggctct cgtcgctaat catgtcgctg tccagatcct ccgcgttgct 19980
cagggcgaat ggggtcatct tgcagacctg cctgcccagg aaaggcggga gcccaggctt 20040
gccgttacag tcgcagcgca ggggcattag caggtgccca cggcccgact gcgcctgcgg 20100
gtacaacgcg cgcatgaagg cttcgatctg cctaaaagcc acctgggtct tggctccctc 20160
cgaaaagaac atcccacagg acttgctgga gaactggttc gcgggacagc tggcatcgtg 20220
caggcagcag cgcgcgtcag tgttggcgat ctgcaccacg ttgcgacccc accggttttt 20280
cactatcttg gccttggaag cctgctcctt tagcgcgcgc tggccgttct cgctggtcac 20340
atccatctct atcacctgtt ccttgttgat catgtttgtc ccgtgcagac actttaggtc 20400
gccctccgtc tgggtgcagc ggtgctccca cagcgcgcaa ccggtgggct cccaattttt 20460
gtgggtcacc cccgcgtagg cctgcaggta ggcctgcagg aagcgcccca tcatggtcat 20520
aaaggtcttc tggctcgtaa aggtcagctg caggccgcga tgctcttcgt tcagccaggt 20580
cttgcagatg gcggccagcg cctcggtctg ctcgggcagc atcttaaaat ttgtcttcag 20640
gtcgttatcc acgtggtact tgtccatcat ggcacgcgcc gcctccatgc ccttctccca 20700
ggcggacacc atgggcaggc ttagggggtt tatcacttcc agcggcgagg acaccgtact 20760
ttcgatttct tcttcctccc cctcttcccg gcgcgcgccc ccgctgttgc gcgctcttac 20820
cgcctgcacc aaggggtcgt cttcaggcaa gcgccgcacc gagcgcttgc cgcccttgac 20880
ctgcttgatc agtaccggcg ggttgctgaa gcccaccatg gtcagcgccg cctgctcttc 20940
ttcgtcttcg ctgtctacca ctatttctgg ggaggggctt ctccgctctg cggcaaaggc 21000
ggcggatcgc ttcttttttt tcttgggagc cgccgcgatg gagtccgcca cggcgaccga 21060
ggtcgagggc gtggggctgg gggtgcgcgg caccagggcc tcgtcgccct cggactcttc 21120
ctctgactcc aggcggcggc ggagtcgctt ctttgggggc gcgcgcgtta gcggcggcgg 21180
agacggggac ggggacgggg acgggacgcc ctccacaggg ggcggtcttc gcgcagaccc 21240
gcggccgcgc tcgggggtct tctcgcgctg gtcttggtcc cgactggcca ttgtatcctc 21300
ctcctcctag gcagagagac ataaggagtc tatcatgcaa gtcgagaagg aggagagctt 21360
aaccaccccc tctgagaccg ccgtcgccgt cgcccccgct accgccgacg cgcccgccac 21420
accgagcgac acccccgcgg acccccccgc cgacgcaccc ctgttcgagg aagcggccgt 21480
ggagcaggac ccgggctttg tctcggcaga ggaggatttg caagaggagg aggataagga 21540
ggagaagccc tcagtgccaa aagatcataa agagcaagac gagcacgacg cagacgcaca 21600
ccagggtgaa gtcgggcggg gggacggagg gcatggcggc gccgactacc tagacgaagg 21660
aaacgacgtg ctcttgaagc acctgcatcg tcagtgcgcc attgtctgcg acgctctgca 21720
ggagcgcagc gaggtgcccc tcagcgtggc ggaggtcagc cgcgcctacg agctcagcct 21780
cttttccccc cgggtgcccc cccgccgccg cgaaaacggc acatgcgagc ccaacccgcg 21840
cctcaacttc taccccgcct ttgtggtgcc cgaggtcctg gccacctatc acatcttctt 21900
tcaaaattgc aagatcccca tctcgtgccg cgccaaccgt agccgcgccg ataagatgct 21960
ggccctgcgc cagggcgacc acatacctga tatcgccgct ttggaagatg tgccaaagat 22020
cttcgagggt ctgggtcgca acgagaagcg ggcagcaaac tctctgcaac aggaaaacag 22080
cgaaaatgag agtcacactg gagcgctggt ggagctggag ggcgacaacg cccgcctggc 22140
ggtgctcaag cgcagcatcg aggtcaccca ctttgcctac cccgcgctca acctgccccc 22200
caaagtcatg aacgcggtca tggacgggct aatcatgcgc cgcggccggc cccttgctcc 22260
agatgcaaac ttgcatgagg agaccgagga cggtcagccc gtggtcagcg acgagcagct 22320
gacgcgctgg ctggaaaccg cggaccccgc cgaactggag gagcggcgca agatgatgat 22380
ggccgcggtg ctggtcaccg tagagctgga gtgtctgcag cgcttcttcg gcgaccccga 22440
gatgcagaga aaggtcgagg agaccctaca ctacaccttc cgccagggct acgtgcgcca 22500
ggcttgcaag atctccaacg tggagctcag caacctggtg tcctacctgg gcatcttgca 22560
tgaaaaccgc cttgggcaga gcgtgctaca ctccaccctg cgcggggagg cgcgccgcga 22620
ctacgtgcgc gactgcgttt acctcttcct ctgctacacc tggcagacgg ccatgggggt 22680
ctggcagcag tgcctggagg agcgcaacct caaggagctg gagaagctcc tgcagcgcgc 22740
gctcaaagac ctctggacgg gctacaacga gcgctcggtg gccgccgcgc tggccgacct 22800
catcttcccc gagcgcctgc tcaaaactct ccagcagggg ctgcccgact tcaccagcca 22860
aagcatgttg caaaatttta ggaactttat cctggagcgt tctggcatcc tacccgccac 22920
ctgctgcgcc ctgcccagcg actttgtccc cctcgtgtac cgcgagtgcc ccccgccgct 22980
gtggggccac tgctacctgt tccaactggc caactacctg tcctaccacg cggacctcat 23040
ggaagactcc agcggcgagg ggctcatgga gtgccactgc cgctgcaacc tctgcacgcc 23100
ccaccgctcc ctggtctgca acacccaact gctcagcgag agtcagatta tcggtacctt 23160
cgagctacag ggtccgtcct cctcagacga gaagtccgcg gctccggggc taaaactcac 23220
tccggggctg tggacttccg cctacctgcg caaatttgta cctgaagact accacgccca 23280
cgagatcagg ttttacgagg accaatcccg cccgcccaag gcggagctga ccgcctgcgt 23340
catcacccag ggcgagatcc taggccaatt gcaagccatc caaaaagccc gccaagagtt 23400
tttgctgaga aagggtcggg gggtgtatct ggacccccag tcgggtgagg agctcaaccc 23460
ggttcccccg ctgccgccgc cgcgggacct tgcttcccag gataagcatc gccatggctc 23520
ccagaaagaa gcagcagcgg ccgccactgc cgccacccca cacgctggag gaagaggagg 23580
aatactggga cagtcaggca gaggaggttt cggacgagga ggagccggag acggagatgg 23640
aagagtggga ggaggacagc ttagacgagg aggcttccga agccgaagag gcaggcgcaa 23700
caccgtcacc ctcggccgca gccccctcgc aggcgccccc gaagtccgct cccagcatca 23760
gcagcaacag cagcgctata acctccgctc ctccaccgcc gcgacccacg gccgaccgca 23820
gacccaaccg tagatgggac accaccggaa ccggggccgg taagtcctcc gggaaaggca 23880
agcaagcgca gcgccaaggc taccgctcgt ggcgcgctca caagaacgcc atagtcgctt 23940
gcttgcaaga ctgcgggggg aacatctcct tcgcccgccg cttcctgctc ttccaccacg 24000
gtgtggcctt cccccgtaac gtcctgcatt actaccgtca tctctacagc ccctactgcg 24060
gcggcagtga gccagaggcg gccggcggca gcggcgcccg tttcggtgcc taggaagacc 24120
cagggcaaga cttcagccaa gaaactcgcg gcggccgcgg cgaacgcggt cgcgggggcc 24180
ctgcgcctga cggtgaacga acccctgtcg acccgcgaac tgaggaaccg aatcttcccc 24240
actctctatg ccatcttcca gcagagcaga gggcaggatc aggaactgaa agtaaaaaac 24300
aggtctctgc gctccctcac ccgcagctgt ctgtatcaca agagcgaaga ccagcttcgg 24360
cgcacgctgg aggacgctga ggcactcttc agcaaatact gcgcgctcac tcttaaggac 24420
tagctccgcg cccttctcga atttaggcgg gaacgcctac gtcatcgcag cgccgccgtc 24480
atgagcaagg acattcccac gccatacatg tggagctatc agccgcagat gggactcgcg 24540
gcgggcgcct cccaggatta ctccacccgc atgaactggc tcagtgccgg cccacacatg 24600
atctcacagg ttaatgacat ccgcacccat cgaaaccaaa tattggtgga gcaggcggca 24660
attaccacca cgccccgcaa taatcccaac cccagggagt ggcccgcgtc cctggtgtat 24720
caggaaattc ccggccccac caccgtacta cttccgcgtg attcccaggc cgaagtccaa 24780
atgactaact caggggcaca gctcgcgggc ggctgtcgtc acagggtgcg gcctcctcgc 24840
cagggtataa ctcacctgaa gatccgaggc agaggtattc agctcaacga cgagtcggtg 24900
agctcctcgc tcggtctcag acctgacggg accttccaga tagccggagc cggccgatct 24960
tccttcacgc cccgccaggc gtacctgact ctgcagagct cgtcctcggc gccgcgctcg 25020
ggcggcatcg ggactctcca gttcgtgcag gagtttgtgc cctcggtcta cttcaacccc 25080
ttctcgggct ctcccggtcg ctacccggac cagttcatcc cgaactttga cgccgcgagg 25140
gactcggtgg acggctacga ctgaatgtcg ggtggacccg gtgcagagca acttcgcctg 25200
aagcaccttg accactgccg ccgccctcag tgctttgccc gctgtcagac cggtgagttc 25260
cagtactttt ccctgcccga ctcgcacccg gacggcccgg cgcacggggt gcgctttttc 25320
atcccgagtc aggtccgctc taccctaatc agggagttca ccgcccgtcc cctactggcg 25380
gagttggaaa aggggccttc tatcctaacc attgcctgca tctgctctaa ccctggatta 25440
caccaagatc tttgctgtca tttgtgtgct gagtataata aaggctgaga tcagaatcta 25500
ctcgggctcc tgtcgccatc ctgtcaacgc caccgtccaa gcccggcccg atcagcccga 25560
ggtgaacctc acctgcggtc tgcaccggcg cctgaggaaa tacctagctt ggtactacaa 25620
cagcactccc tttgtggttt acaacagctt tgaccaggac ggggtctcac tgagggataa 25680
cctctcgaac ctgagctact ccatcaggaa gaacaacacc ctcgagctac ttcctcctta 25740
cctgcccggg acttaccagt gtgtcaccgg tccctgcacc cacacccacc tgttgatcgt 25800
aaacgactct cttccgagaa cagacctcaa taactcctct ccgcagttcc ccagaacagg 25860
aggtgagctc aggaaacccc gggtaaagaa gggtggacaa gagttaacac ttgtggggtt 25920
tctggtgtat gtgacgctgg tggtggctct tttgattaag gcttttcctt ccatgtctga 25980
actatccctc ttcttttatg aacaactcga ctagtgctaa cgggacccta cccaacgaat 26040
cgggattgaa tatcggtaac caggttgcag tttcactttt gattaccttc atagtcctct 26100
tcctgctagt gctgtcgctt ctgtgcctgc ggatcggggg ctgctgcatc cacgtttata 26160
tctggtgctg gctgtttaga aggttcggag accaccgcag gtagaataaa caaacctaga 26220
cctagaaatg gacggtctct gcagcgagca acgcatacta gagaggcgcc ggcaaaaagc 26280
agagctcgag cgtcttaaac aagagctcca agacgccgtg gccatacacc agtgcaaaaa 26340
agggctcttc tgtctggtaa aacaggccac gctcacctat gaaaaaacag gtgacaccca 26400
ccgcctagga tacaagctgc ccacacagcg ccaaaagttt gcccttatga taggtgaaca 26460
acccatcacc gtgacccagc actccgtgga gacagaaggc tgcattcatg ctccctgcag 26520
gggcgctgac tgcctctaca ccttgatcaa aaccctttgc ggtctcagag accttatccc 26580
tttcaattga tcataactgt aatcaataaa aaatcactta cttgaaatct gatagcaagc 26640
ctctgtccaa ttttttcagc aacacttcct tcccctcttc ccaactctgg tactctaggc 26700
gcctcctagc tgcaaacttc ctccacagtc tgaagggaat gtcagattcc tcctcctcct 26760
gtccctccgc acccacgatc ttcatgttgt tgcagatgaa gcgcaccaaa acgtctgacg 26820
agagcttcaa ccccgtgtac ccctatgaca cggaaaacgg tcctccctcc gtccctttcc 26880
tcacccctcc cttcgtgtct cccgatggat tccaagagag cccccccggg gtcctgtctc 26940
tgaacctggc cgagcccctg gtcacttccc acggcatgct cgccctgaaa atgggaagtg 27000
gcctctccct ggacgacgcc ggcaacctca cctctcaaga tgtcaccacc actacccctc 27060
ccctgaaaaa aaccaagacc aacctcagcc tagaaacctc agcccccctg actgtgagca 27120
cctcaggcgc cctcacccta gcagccgccg ttcccctggc ggtggccggc acctccctca 27180
ccatgcaatc agaggccccc ctgacagtcc aagatgcaaa actcaccctg gccaccaagg 27240
gccccctgac cgtgtctgaa ggcaaactgg ccttgcagac ctcggccccg ctgacggccg 27300
ctgacagcag cgccctcacc gttagcgcca caccacccat cagtgtaagc agtggaagtt 27360
tgggcttaga catggaagac cccatgtata ctcatgatgg aaaactggga ataagaattg 27420
gaggcccact gagagtagta gacagcctgc acacactgac tgtagttacc ggaaatggaa 27480
tagctgtaga taacaatgcc ctccaaacta gagttacggg cgccctgggt tatgacacat 27540
caggaaacct acaactgaga gccgcggggg gtatgcgaat tgatgcaaat ggccaactta 27600
tccttgatgt ggcataccca tttgatgctc aaaacaatct cagccttaga cttggtcagg 27660
gacccctgta tgtaaacaca gaccacaacc tagatttgaa ttgcaacaga ggtctgacca 27720
caactaccac caacaacaca aaaaaacttg aaactaaaat tggctcaggc ttagactatg 27780
ataccaatgg tgctgtcatt attaaacttg gtactggtgt aagctttgac agcacaggcg 27840
ccctaactgt gggaaacact ggcgatgata aactgactct gtggacaacc ccagacccat 27900
ctccaaattg cagaattcac gcagacaaag actgcaagtt tactctagtc ctaactaagt 27960
gtggaagtca aatcctggct tctgtcgccg ccctagcggt gtcaggaaat ctggcttcaa 28020
taacaggcac cgttgccagc gttaccatct ttctcagatt tgatcagaat ggagtgctta 28080
tggaaaactc ctccctagac aagcagtact ggaacttcag aaatggtaac tcaaccaatg 28140
ccacccccta caccaatgca gttgggttca tgccaaacct cgcagcatac cccaagacac 28200
agagccagac tgctaaaaac aacattgtaa gtcaggttta cttgaatggg gacaaatcca 28260
aacccatgac ccttaccatt accctcaatg gaactaatga atccagtgaa actagccagg 28320
tgagtcacta ctccatgtca tttacatggg cttgggagag tgggcaatat gccaccgaaa 28380
cctttgccac caattccttt accttctctt acattgctga acaataaaga aagcacagag 28440
atgcttgttt ttgatttcaa aattgtgtgc ttttatttat tttcaagctt acagtatttc 28500
cagtagtcat tcgaatagag cttaatgaaa ctgcatgaga acccttccac atagcttaaa 28560
ttatcaccag tgcaaatgga gaaaaatcaa catacctttt tatccagata tcacagaacc 28620
ctagtattca acctgccacc tccctcccaa cacacagagt acacagtcct ttctccccgg 28680
ctggccttaa aaagcatcat atcatgggta acagacatat tcttaggtgt tatattccac 28740
acggtttcct gtcgagccaa acgctcatca gtgatattaa taaactcccc gggcagctca 28800
cttaagttca tgtcgctgtc cagctgctga gccacaggct gctgtccaac ttgcggttgc 28860
ttaacgggcg gcgaaggaga agtccacgcc tacatggggg tagagtcata atcgtgcatc 28920
aggatagggc ggtggtgctg cagcagcgcg cgaataaact gctgccgccg ccgctccgtc 28980
ctgcaggaat acaacatggc agtggtctcc tcagcgatga ttcgcaccgc ccgcagcata 29040
aggcgccttg tcctccgggc acagcagcgc accctgatct cacttaaatc agcacagtaa 29100
ctgcagcaca gcaccacaat attgttcaaa atcccacagt gcaaggcgct gtatccaaag 29160
ctcatggcgg ggaccacaga acccacgtgg ccatcatacc acaagcgcag gtagattaag 29220
tggcgacccc tcataaacac gctggacata aacattacct cttttggcat gttgtaattc 29280
accacctccc ggtaccatat aaacctctga ttaaacatgg cgccatccac caccatccta 29340
aaccagctgg ccaaaacctg cccgccggct atacactgca gggaaccggg actggaacaa 29400
tgacagtgga gagcccagga ctcgtaacca tggatcatca tgctcgtcat gatatcaatg 29460
ttggcacaac acaggcacac gtgcatacac ttcctcagga ttacaagctc ctcccgcgtt 29520
agaaccatat cccagggaac aacccattcc tgaatcagcg taaatcccac actgcaggga 29580
agacctcgca cgtaactcac gttgtgcatt gtcaaagtgt tacattcggg cagcagcgga 29640
tgatcctcca gtatggtagc gcgggtttct gtctcaaaag gaggtagacg atccctactg 29700
tacggagtgc gccgagacaa ccgagatcgt gttggtcgta gtgtcatgcc aaatggaacg 29760
ccggacgtag tcatatttcc tccagcagaa ccaagtgcgc gcgtggcagc tatccttgcg 29820
tcttctgtct cgccgcctgc cccgctcggt gtagtagttg taatacagcc actccctcag 29880
accgtcaagg cgctccctgg cgtccggatc tataacaaca ccatcctgca gcgccgccct 29940
gatgacatcc accaccgtag agtatgccaa gcccagccag gaaatgcact cactttgaca 30000
gcgagagata ggaggagcgg gaagagatgg aagaaccatg atagtaaaag aacttttatt 30060
ccaatcgatc ctctacaatg tcaaagtgta gatctatcag atggcactgg tctcctccgc 30120
tgagtcgatc aaaaataaca gctaaaccac aaacaacacg attggtcaaa tgctgcacaa 30180
gggcttgcag cataaaatcg cctcgaaagt ccaccgcaag cataacatca aagccaccgc 30240
ccctatcatg atctatgata aaaaccccac agctatccac cagacccata tagttttcat 30300
ctctccatcg tgaaaaaata tttacaagct cctcctttaa atcacctcca accaattcaa 30360
aaagttgagc cagaccgccc tccaccttca ttttcagcat gcgcatcatg attgcaaaaa 30420
ttcaggctcc tcagacacct gtataagatt gagaagcgga acattaacat caatgtttcg 30480
ctcgcgaaga tcgcgcctca gtgcaagcat gatataatcc cacaggtcgg agcggatcag 30540
cgaggacatc tccccgccag gaaccaactc aacggagcct atgctgatta taatacgcat 30600
attcggggct atgctaacca gcacggcccc caaataggcg tactgcatag gcggcgacaa 30660
aaagtgaaca gtttgggtta aaaaatcagg caaacactcg cgcaaaaaag caagaacatc 30720
ataaccatgc tcatgcaaat agatgcaagt aagctcagga acgaccacag aaaaatgcac 30780
aatttttctc tcaaacatga ctgcgagccc tgcaaaaaat aaaaaagaaa cattacacaa 30840
gagtagcctg tcttacaatg ggatagacta ctctaaccaa cataagacgg gccacgacat 30900
cgcccgcgtg gccataaaaa aaattatccg tgtgattaaa aagaagcaca gatagctggc 30960
cagtcatatc cggagtcatc acgtgcgaac ccgtgtagac ccccgggttg gacacatcgg 31020
ccaaagaaag aaagcggcca atgtatcccg gaggaatgat aacactaaga cgaagataca 31080
acagaataac cccatggggg ggaataacaa agttagtagg tgaataaaaa cgataaacac 31140
ccgaaactcc ctcctgcgta ggcaaaatag cgccctcccc ttccaaaaca acatacagcg 31200
cttccacagc agccatgaca aaagactcaa aacactcaaa agactcagtc ttaccaggaa 31260
aataaaagca ctctcacagc accagcacta atcagagtgt gaagagggcc aagtgccgaa 31320
cgagtatata taggaattaa aaatgacgta aatgtgtaaa ggtcaaaaaa cgcccagaaa 31380
aatacacaga ccaacgcccg aaacgaaaac ccgcgaaaaa atacccagaa gttcctcaac 31440
aaccgccact tccgctttcc cacgatacgt cacttcctcg aaaatagcaa actacatttc 31500
ccacatgtac aaaaccgaaa ccactcccct tgtcaccgcc cacaacttac atcttaatta 31560
acaaacgtca aagcctacgt cagccgcccc gcctcgcccc gcccacctca ttatcatatt 31620
ggccacaatc caaaataagg tatattattg atgatg 31656
<210> 13
<211> 10158
<212> DNA
<213> 人工序列
<220>
<223> BZ1/BZ28中间质粒1
<400> 13
catcatcaat aatatacctt attttggatt gtggccaata tgataatgag gtgggcgggg 60
cgaggcgggg cgggtgacgt aggacgtgcg agtagggttg ggaggtgtgg cggaagtgtg 120
gcatttgtaa gtgggaggag cttacatgca agcttccgtc gcggaaaatg tgacgttttt 180
tatgagcgcc gcctacctcc ggaagtgcca attttcgcgc gctttttacc ggatatcgta 240
gtaattttgg gcgggaccat gtaagatttg gccattttcg cgcgaaaagt gaaacgggga 300
agtgaaaact gaataatagg gcgttagtca tagcgcgtaa tatttaccga gggccgaggg 360
actttgaccg attacgtgga ggactcgccc aggtgttttt tacgtgaatt tccgcgttcc 420
gggtcaaagt ctccgttttt attgtcaccg tcatttgacg cggagggtat ttaaacccgc 480
tgcgctccta gcgatcgctg atgagaccag gaccaggtgc cgaccctgcg agtgcggcgg 540
caagcacatg agaaatcagc ctgtgatgtt ggatgtgacc gaggagctta ggcctgacca 600
tctggtgctg gcctgcacca gggccgagtt tgggtctagc gatgaggata ccgattgagg 660
tgggtaaggt gggcgtggct agcagggtgg gcgtgtataa attgggggtc taaggggtct 720
ctctgtttgt cttgcaacag ccgccgcatg agcgacaccg gcaacagctt tgatggaagc 780
atctttagcc cctatctgac agtgcgcatg cctcactggg ccggagtgcg tcagaatgtg 840
atgggttcca acgtggatgg acgtcccgtt ctgccttcaa attcgtctac gatggcctac 900
gcgaccgtgg gaggaactcc gctggacgcc gcgacctccg ccgccgcctc cgccgccgcc 960
gcgaccgcgc gcagcatggc tacggacctt tacagctctt tggtggcgag cagcgcggcc 1020
tctcgcgcgt ctgctcggga tgaaaaactg actgctctgc tgcttaaact ggaagacttg 1080
acccgggagc tgggtcaact gacccagcag gtctccagct tgcgtgagag cagccttgcc 1140
tccccctaat ggcccataat ataaataaaa gccagtctgt ttggattaag caagtgtatg 1200
ttctttattt aactctccgc gcgcggtaag cccgggacca gcggtctcgg tcgtttaggg 1260
tgcggtggat tctttccaac acgtggtaca ggtggctctg gatgtttaga tacatgggca 1320
tgagtccatc cctggggtgg aggtagcacc actgcagagc ttcgtgctcg ggggtggtgt 1380
tgtatatgat ccagtcgtag caggagcgct gggcgtggtg ctgaaaaatg tccttaagca 1440
agaggcttat agctaggggg aggcccttgg tgtaagtgtt tacaaatctg cttagctggg 1500
aggggtgcat ccggggggat atgatgtgca tcttggactg gatttttagg ttggctatgt 1560
tcccacccag atcccttctg ggattcatgt tgtgcaggac caccagcacg gtatatccag 1620
tgcacttggg aaatttatcg tggagcttag acgggaatgc atggaagaac ttggagacgc 1680
ccttgtggcc tcccagattt tccatacatt cgtccatgat gatggcaatg ggcccgtggg 1740
aagctgcctg agcaaaaacg tttctggcat cgctcacatc gtagttatgt tccagggtga 1800
ggtcatcata ggacatcttt acaaatcggg ggcggagggt cccggactgg gggatgatgg 1860
tacgtattgg tcgctgccaa acatgatgca agacccggtg accttccgct ccacgcggca 1920
ggttagcaac ttcccggtgg tgggcgccga actgctgccc gtgcactcca agagttttta 1980
caacgagcag gccgtctact cccagctgat ccgccaggcc acctctctga cccacgtgtt 2040
caatcgcttt cccgagaacc agattttggc gcgcccgccg gcccccacca tcaccaccgt 2100
cagtgaaaac gttcctgccc tcacagatca cgggacgcta ccgctgcgca acagcatctc 2160
aggagtccag cgagtgacca ttactgacgc cagacgccgg acctgcccct acgtttaaac 2220
gactctcttc cgagaacaga cctcaataac tcctctccgc agttccccag aacaggaggt 2280
gagctcagga aaccccgggt aaagaagggt ggacaagagt taacacttgt ggggtttctg 2340
gtgtatgtga cgctggtggt ggctcttttg attaaggctt ttccttccat gtctgaacta 2400
tccctcttct tttatgaaca actcgactag tgctaacggg accctaccca acgaatcggg 2460
attgaatatc ggtaaccagg ttgcagtttc acttttgatt accttcatag tcctcttcct 2520
gctagtgctg tcgcttctgt gcctgcggat cgggggctgc tgcatccacg tttatatctg 2580
gtgctggctg tttagaaggt tcggagacca ccgcaggtag aataaacaaa cctagaccta 2640
gaaatggacg gtctctgcag cgagcaacgc atactagaga ggcgccggca aaaagcagag 2700
ctcgagcgtc ttaaacaaga gctccaagac gccgtggcca tacaccagtg caaaaaaggg 2760
ctcttctgtc tggtaaaaca ggccacgctc acctatgaaa aaacaggtga cacccaccgc 2820
ctaggataca agctgcccac acagcgccaa aagtttgccc ttatgatagg tgaacaaccc 2880
atcaccgtga cccagcactc cgtggagaca gaaggctgca ttcatgctcc ctgcaggggc 2940
gctgactgcc tctacacctt gatcaaaacc ctttgcggtc tcagagacct tatccctttc 3000
aattgatcat aactgtaatc aataaaaaat cacttacttg aaatctgata gcaagcctct 3060
gtccaatttt ttcagcaaca cttccttccc ctcttcccaa ctctggtact ctaggcgcct 3120
cctagctgca aacttcctcc acagtctgaa gggaatgtca gattcctcct cctcctgtcc 3180
ctccgcaccc acgatcttca tgttgttgca gatgaagcgc accaaaacgt ctgacgagag 3240
cttcaacccc gtgtacccct atgacacgga aaacggtcct ccctccgtcc ctttcctcac 3300
ccctcccttc gtgtctcccg atggattcca agagagcccc cccggggtcc tgtctctgaa 3360
cctggccgag cccctggtca cttcccacgg catgctcgcc ctgaaaatgg gaagtggcct 3420
ctccctggac gacgccggca acctcacctc tcaagatgtc accaccacta cccctcccct 3480
gaaaaaaacc aagaccaacc tcagcctaga aacctcagcc cccctgactg tgagcacctc 3540
aggcgccctc accctagcag ccgccgttcc cctggcggtg gccggcacct ccctcaccat 3600
gcaatcagag gcccccctga cagtccaaga tgcaaaactc accctggcca ccaagggccc 3660
cctgaccgtg tctgaaggca aactggcctt gcagacctcg gccccgctga cggccgctga 3720
cagcagcgcc ctcaccgtta gcgccacacc acccatcagt gtaagcagtg gaagtttggg 3780
cttagacatg gaagacccca tgtatactca tgatggaaaa ctgggaataa gaattggagg 3840
cccactgaga gtagtagaca gcctgcacac actgactgta gttaccggaa atggaatagc 3900
tgtagataac aatgccctcc aaactagagt tacgggcgcc ctgggttatg acacatcagg 3960
aaacctacaa ctgagagccg cggggggtat gcgaattgat gcaaatggcc aacttatcct 4020
tgatgtggca tacccatttg atgctcaaaa caatctcagc cttagacttg gtcagggacc 4080
cctgtatgta aacacagacc acaacctaga tttgaattgc aacagaggtc tgaccacaac 4140
taccaccaac aacacaaaaa aacttgaaac taaaattggc tcaggcttag actatgatac 4200
caatggtgct gtcattatta aacttggtac tggtgtaagc tttgacagca caggcgccct 4260
aactgtggga aacactggcg atgataaact gactctgtgg acaaccccag acccatctcc 4320
aaattgcaga attcacgcag acaaagactg caagtttact ctagtcctaa ctaagtgtgg 4380
aagtcaaatc ctggcttctg tcgccgccct agcggtgtca ggaaatctgg cttcaataac 4440
aggcaccgtt gccagcgtta ccatctttct cagatttgat cagaatggag tgcttatgga 4500
aaactcctcc ctagacaagc agtactggaa cttcagaaat ggtaactcaa ccaatgccac 4560
cccctacacc aatgcagttg ggttcatgcc aaacctcgca gcatacccca agacacagag 4620
ccagactgct aaaaacaaca ttgtaagtca ggtttacttg aatggggaca aatccaaacc 4680
catgaccctt accattaccc tcaatggaac taatgaatcc agtgaaacta gccaggtgag 4740
tcactactcc atgtcattta catgggcttg ggagagtggg caatatgcca ccgaaacctt 4800
tgccaccaat tcctttacct tctcttacat tgctgaacaa taaagaaagc acagagatgc 4860
ttgtttttga tttcaaaatt gtgtgctttt atttattttc aagcttacag tatttccagt 4920
agtcattcga atagagctta atgaaactgc atgagaaccc ttccacatag cttaaattat 4980
caccagtgca aatggagaaa aatcaacata cctttttatc cagatatcac agaaccctag 5040
tattcaacct gccacctccc tcccaacaca cagagtacac agtcctttct ccccggctgg 5100
ccttaaaaag catcatatca tgggtaacag acatattctt aggtgttata ttccacacgg 5160
tttcctgtcg agccaaacgc tcatcagtga tattaataaa ctccccgggc agctcactta 5220
agttcatgtc gctgtccagc tgctgagcca caggctgctg tccaacttgc ggttgcttaa 5280
cgggcggcga aggagaagtc cacgcctaca tgggggtaga gtcataatcg tgcatcagga 5340
tagggcggtg gtgctgcagc agcgcgcgaa taaactgctg ccgccgccgc tccgtcctgc 5400
aggaatacaa catggcagtg gtctcctcag cgatgattcg caccgcccgc agcataaggc 5460
gccttgtcct ccgggcacag cagcgcaccc tgatctcact taaatcagca cagtaactgc 5520
agcacagcac cacaatattg ttcaaaatcc cacagtgcaa ggcgctgtat ccaaagctca 5580
tggcggggac cacagaaccc acgtggccat cataccacaa gcgcaggtag attaagtggc 5640
gacccctcat aaacacgctg gacataaaca ttacctcttt tggcatgttg taattcacca 5700
cctcccggta ccatataaac ctctgattaa acatggcgcc atccaccacc atcctaaacc 5760
agctggccaa aacctgcccg ccggctatac actgcaggga accgggactg gaacaatgac 5820
agtggagagc ccaggactcg taaccatgga tcatcatgct cgtcatgata tcaatgttgg 5880
cacaacacag gcacacgtgc atacacttcc tcaggattac aagctcctcc cgcgttagaa 5940
ccatatccca gggaacaacc cattcctgaa tcagcgtaaa tcccacactg cagggaagac 6000
ctcgcacgta actcacgttg tgcattgtca aagtgttaca ttcgggcagc agcggatgat 6060
cctccagtat ggtagcgcgg gtttctgtct caaaaggagg tagacgatcc ctactgtacg 6120
gagtgcgccg agacaaccga gatcgtgttg gtcgtagtgt catgccaaat ggaacgccgg 6180
acgtagtcat atttcctcca gcagaaccaa gtgcgcgcgt ggcagctatc cttgcgtctt 6240
ctgtctcgcc gcctgccccg ctcggtgtag tagttgtaat acagccactc cctcagaccg 6300
tcaaggcgct ccctggcgtc cggatctata acaacaccat cctgcagcgc cgccctgatg 6360
acatccacca ccgtagagta tgccaagccc agccaggaaa tgcactcact ttgacagcga 6420
gagataggag gagcgggaag agatggaaga accatgatag taaaagaact tttattccaa 6480
tcgatcctct acaatgtcaa agtgtagatc tatcagatgg cactggtctc ctccgctgag 6540
tcgatcaaaa ataacagcta aaccacaaac aacacgattg gtcaaatgct gcacaagggc 6600
ttgcagcata aaatcgcctc gaaagtccac cgcaagcata acatcaaagc caccgcccct 6660
atcatgatct atgataaaaa ccccacagct atccaccaga cccatatagt tttcatctct 6720
ccatcgtgaa aaaatattta caagctcctc ctttaaatca cctccaacca attcaaaaag 6780
ttgagccaga ccgccctcca ccttcatttt cagcatgcgc atcatgattg caaaaattca 6840
ggctcctcag acacctgtat aagattgaga agcggaacat taacatcaat gtttcgctcg 6900
cgaagatcgc gcctcagtgc aagcatgata taatcccaca ggtcggagcg gatcagcgag 6960
gacatctccc cgccaggaac caactcaacg gagcctatgc tgattataat acgcatattc 7020
ggggctatgc taaccagcac ggcccccaaa taggcgtact gcataggcgg cgacaaaaag 7080
tgaacagttt gggttaaaaa atcaggcaaa cactcgcgca aaaaagcaag aacatcataa 7140
ccatgctcat gcaaatagat gcaagtaagc tcaggaacga ccacagaaaa atgcacaatt 7200
tttctctcaa acatgactgc gagccctgca aaaaataaaa aagaaacatt acacaagagt 7260
agcctgtctt acaatgggat agactactct aaccaacata agacgggcca cgacatcgcc 7320
cgcgtggcca taaaaaaaat tatccgtgtg attaaaaaga agcacagata gctggccagt 7380
catatccgga gtcatcacgt gcgaacccgt gtagaccccc gggttggaca catcggccaa 7440
agaaagaaag cggccaatgt atcccggagg aatgataaca ctaagacgaa gatacaacag 7500
aataacccca tgggggggaa taacaaagtt agtaggtgaa taaaaacgat aaacacccga 7560
aactccctcc tgcgtaggca aaatagcgcc ctccccttcc aaaacaacat acagcgcttc 7620
cacagcagcc atgacaaaag actcaaaaca ctcaaaagac tcagtcttac caggaaaata 7680
aaagcactct cacagcacca gcactaatca gagtgtgaag agggccaagt gccgaacgag 7740
tatatatagg aattaaaaat gacgtaaatg tgtaaaggtc aaaaaacgcc cagaaaaata 7800
cacagaccaa cgcccgaaac gaaaacccgc gaaaaaatac ccagaagttc ctcaacaacc 7860
gccacttccg ctttcccacg atacgtcact tcctcgaaaa tagcaaacta catttcccac 7920
atgtacaaaa ccgaaaccac tccccttgtc accgcccaca acttacatct taattaacaa 7980
acgtcaaagc ctacgtcagc cgccccgcct cgccccgccc acctcattat catattggcc 8040
acaatccaaa ataaggtata ttattgatga tgatttaaat attatgcggt gtgaaatacc 8100
gcacagatgc gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga 8160
ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 8220
acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 8280
aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 8340
tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 8400
aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 8460
gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 8520
acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 8580
accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 8640
ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 8700
gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 8760
gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 8820
ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 8880
gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 8940
cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 9000
cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 9060
gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 9120
tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 9180
gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 9240
agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 9300
tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 9360
agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc 9420
gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 9480
catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 9540
ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 9600
atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 9660
tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag 9720
cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 9780
cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 9840
atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 9900
aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 9960
ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 10020
aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga 10080
aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct 10140
tcaagaattg atttaaat 10158
<210> 14
<211> 2927
<212> DNA
<213> 人工序列
<220>
<223> BZ1/BZ28.5IXP腺病毒载体片段
<400> 14
gcgatcgcgg tactgaaatg tgtgggcgtg gcttaagggt gggaaagaat atataaggtg 60
ggggtcttat gtagttttgt atctgttttg cagcagccgc cgccgccatg agcgacaccg 120
gcaacagctt tgatggaagc atctttagcc cctatctgac agtgcgcatg cctcactggg 180
ccggagtgcg tcagaatgtg atgggttcca acgtggatgg acgtcccgtt ctgccttcaa 240
attcgtctac gatggcctac gcgaccgtgg gaggaactcc gctggacgcc gcgacctccg 300
ccgccgcctc cgccgccgcc gcgaccgcgc gcagcatggc tacggacctt tacagctctt 360
tggtggcgag cagcgcggcc tctcgcgcgt ctgctcggga tgaaaaactg actgctctgc 420
tgcttaaact ggaagacttg acccgggagc tgggtcaact gacccagcag gtctccagct 480
tgcgtgagag cagccttgcc tccccctaat ggcccataat ataaataaaa gccagtctgt 540
ttggattaag caagtgtatg ttctttattt aactctccgc gcgcggtaag cccgggacca 600
gcggtctcgg tcgtttaggg tgcggtggat tctttccaac acgtggtaca ggtggctctg 660
gatgtttaga tacatgggca tgagtccatc cctggggtgg aggtagcacc actgcagagc 720
ttcgtgctcg ggggtggtgt tgtatatgat ccagtcgtag caggagcgct gggcgtggtg 780
ctgaaaaatg tccttaagca agaggcttat agctaggggg aggcccttgg tgtaagtgtt 840
tacaaatctg cttagctggg aggggtgcat ccggggggat atgatgtgca tcttggactg 900
gatttttagg ttggctatgt tcccacccag atcccttctg ggattcatgt tgtgcaggac 960
caccagcacg gtatatccag tgcacttggg aaatttatcg tggagcttag acgggaatgc 1020
atggaagaac ttggagacgc ccttgtggcc tcccagattt tccatacatt cgtccatgat 1080
gatggcaatg ggcccgtggg aagctgcctg agcaaaaacg tttctggcat cgctcacatc 1140
gtagttatgt tccagggtga ggtcatcata ggacatcttt acaaatcggg ggcggagggt 1200
cccggactgg gggatgatgg taccctcggg ccccggggcg tagttcccct cacagatctg 1260
catctcccag gctttcattt cagagggagg gatcatatcc acctgcgggg cgatgaaaaa 1320
gacagtttct ggcgcagggg agattaactg ggatgagagc aggtttctga gcagctgtga 1380
ctttccacag ccggtgggcc catatatcac gcctatcacc ggctgcagct ggtagttaag 1440
agagctgcag ctgccgtcct cccggagcag gggggccacc tcgttgagca tatccctgac 1500
gtggatgttc tccctgacca gttccgccag aaggcgctcg ccgcccagcg aaagcagctc 1560
ttgcaaggaa gcaaaatttt tcagcggttt caggccatcg gccgtgggca tgtttttcag 1620
cgtctgggtc agcagctcca gcctgtccca gagctcggtg atgtgctcta cggcatctcg 1680
atccagcaga tctcctcgtt tcgcgggttg gggcggcttt cgctgtaggg caccagccga 1740
tgggcgtcca gcggggccag agtcatgtcc ttccatgggc gcagagtcct cgtcagggtg 1800
gtctgggtca cggtgaaggg gtgcgctccg ggttgggcac tggccagggt gcgcttgagg 1860
ctggttctgc tggtgctgaa tcgctgccgc tcttcgccct gcgcgtcggc caggtagcat 1920
ttgaccatgg tctcgtagtc gagaccctcg gcggcgtgcc ccttggcgcg gagctttccc 1980
ttggaggtgg cgccgcacga ggggcactgc aggctcttca gggcgtagag cttgggagcg 2040
agaaacacgg actctgggga gtaggcgtcc gcgccgcagg ccgcgcagac cgtctcgcat 2100
tccaccagcc aagtgagttc cgggcggtca gggtcaaaaa ccaggttgcc cccatgcttt 2160
ttgatgcgtt tcttacctcg gctctccatg aggcggtgtc ccttctcggt gacgaagagg 2220
ctgtccgtgt ccccgtagac cgacttcagg ggcctgtctt ccagcggagt gcctctgtcc 2280
tcctcgtaga gaaactctga ccactctgag acgaaggccc gtgtccaggc caggacgaag 2340
gaggccacgt gggaggggta gcggtcgttg tccactagcg ggtccacctt ctccagggtg 2400
tgcagacaca tgtccccctc ctccgcgtcc agaaaagtga ttggcttata ggtgtaggac 2460
acgtgaccgg gggttcccga cgggggggta taaaaggggg tgggcgccct ttcatcttca 2520
ctctcttccg catcgctgtc tgcgagagcc agctgctggg gtaagtattc cctttcaaag 2580
gcgggcatga cctcagcgct caggttgtca gtttctaaaa atgaggagga tttgatgttc 2640
acctgtccgg aagtgatacc tttgagggta cctgggtcca tctggtcaga aaacactatt 2700
tttttgttgt caagcttggt ggcgaacgac ccgtagaggg cgttggagag cagcttggca 2760
atggagcgca gggtctggtt tttgtcgcgg tcggctcgct ccttggccgc gatgttgagt 2820
tgcacgtatt cgcgggccac gcacttccac tcggggaaga cggtggtgcg ctcgtctggg 2880
atcaggcgca ccctccagcc gcggttgtgc agggtgacca tgtcgac 2927
<210> 15
<211> 36563
<212> DNA
<213> 人工序列
<220>
<223> pBZ1_BZ28F.Fluc
<400> 15
catcatcaat aatatacctt attttggatt gtggccaata tgataatgag gtgggcgggg 60
cgaggcgggg cgggtgacgt aggacgtgcg agtagggttg ggaggtgtgg cggaagtgtg 120
gcatttgtaa gtgggaggag cttacatgca agcttccgtc gcggaaaatg tgacgttttt 180
tatgagcgcc gcctacctcc ggaagtgcca attttcgcgc gctttttacc ggatatcgta 240
gtaattttgg gcgggaccat gtaagatttg gccattttcg cgcgaaaagt gaaacgggga 300
agtgaaaact gaataatagg gcgttagtca tagcgcgtaa tatttaccga gggccgaggg 360
actttgaccg attacgtgga ggactcgccc aggtgttttt tacgtgaatt tccgcgttcc 420
gggtcaaagt ctccgttttt attgtcaccg tcatttgacg cggagggtat ttaaacccgc 480
tgcgctccta gcgatcgctc aatattggcc attagccata ttattcattg gttatatagc 540
ataaatcaat attggctatt ggccattgca tacgttgtat ccatatcata atatgtacat 600
ttatattggc tcatgtccaa cattaccgcc atgttgacat tgattattga ctagttatta 660
atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata 720
acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat 780
aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga 840
gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc 900
ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt 960
atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat 1020
gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag 1080
tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc 1140
aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga 1200
ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg 1260
ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggcc gggaacggtg 1320
cattggaagc ttggcattcc ggtactgttg gtaaagccac catggaagac gccaaaaaca 1380
taaagaaagg cccggcgcca ttctatccgc tggaagatgg aaccgctgga gagcaactgc 1440
ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata 1500
tcgaggtgga catcacttac gctgagtact tcgaaatgtc cgttcggttg gcagaagcta 1560
tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc 1620
aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg 1680
acatttataa tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct accgtggtgt 1740
tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc ccaatcatcc 1800
aaaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg atgtacacgt 1860
tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtgcca gagtccttcg 1920
atagggacaa gacaattgca ctgatcatga actcctctgg atctactggt ctgcctaaag 1980
gtgtcgctct gcctcataga actgcctgcg tgagattctc gcatgccaga gatcctattt 2040
ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc catcacggtt 2100
ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc ttaatgtata 2160
gatttgaaga agagctgttt ctgaggagcc ttcaggatta caagattcaa agtgcgctgc 2220
tggtgccaac cctattctcc ttcttcgcca aaagcactct gattgacaaa tacgatttat 2280
ctaatttaca cgaaattgct tctggtggcg ctcccctctc taaggaagtc ggggaagcgg 2340
ttgccaagag gttccatctg ccaggtatca ggcaaggata tgggctcact gagactacat 2400
cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt aaagttgttc 2460
cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc gttaatcaaa 2520
gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac aatccggaag 2580
cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata gcttactggg 2640
acgaagacga acacttcttc atcgttgacc gcctgaagtc tctgattaag tacaaaggct 2700
atcaggtggc tcccgctgaa ttggaatcca tcttgctcca acaccccaac atcttcgacg 2760
caggtgtcgc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc gttgttgttt 2820
tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa 2880
caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta 2940
ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag aagggcggaa 3000
agatcgccgt gtaattctag acgagatccg aacttgttta ttgcagctta taatggttac 3060
aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 3120
tgtggtttgt ccaaactcat caatgtatct tatcatgtct gcgatcgctg atgagaccag 3180
gaccaggtgc cgaccctgcg agtgcggcgg caagcacatg agaaatcagc ctgtgatgtt 3240
ggatgtgacc gaggagctta ggcctgacca tctggtgctg gcctgcacca gggccgagtt 3300
tgggtctagc gatgaggata ccgattgagg tgggtaaggt gggcgtggct agcagggtgg 3360
gcgtgtataa attgggggtc taaggggtct ctctgtttgt cttgcaacag ccgccgccat 3420
gagcgacacc ggcaacagct ttgatggaag catctttagc ccctatctga cagtgcgcat 3480
gcctcactgg gccggagtgc gtcagaatgt gatgggttcc aacgtggatg gacgtcccgt 3540
tctgccttca aattcgtcta cgatggccta cgcgaccgtg ggaggaactc cgctggacgc 3600
cgcgacctcc gccgccgcct ccgccgccgc cgcgaccgcg cgcagcatgg ctacggacct 3660
ttacagctct ttggtggcga gcagcgcggc ctctcgcgcg tctgctcggg atgaaaaact 3720
gactgctctg ctgcttaaac tggaagactt gacccgggag ctgggtcaac tgacccagca 3780
ggtctccagc ttgcgtgaga gcagccttgc ctccccctaa tggcccataa tataaataaa 3840
agccagtctg tttggattaa gcaagtgtat gttctttatt taactctccg cgcgcggtaa 3900
gcccgggacc agcggtctcg gtcgtttagg gtgcggtgga ttctttccaa cacgtggtac 3960
aggtggctct ggatgtttag atacatgggc atgagtccat ccctggggtg gaggtagcac 4020
cactgcagag cttcgtgctc gggggtggtg ttgtatatga tccagtcgta gcaggagcgc 4080
tgggcgtggt gctgaaaaat gtccttaagc aagaggctta tagctagggg gaggcccttg 4140
gtgtaagtgt ttacaaatct gcttagctgg gaggggtgca tccgggggga tatgatgtgc 4200
atcttggact ggatttttag gttggctatg ttcccaccca gatcccttct gggattcatg 4260
ttgtgcagga ccaccagcac ggtatatcca gtgcacttgg gaaatttatc gtggagctta 4320
gacgggaatg catggaagaa cttggagacg cccttgtggc ctcccagatt ttccatacat 4380
tcgtccatga tgatggcaat gggcccgtgg gaagctgcct gagcaaaaac gtttctggca 4440
tcgctcacat cgtagttatg ttccagggtg aggtcatcat aggacatctt tacaaatcgg 4500
gggcggaggg tcccggactg ggggatgatg gtaccctcgg gccccggggc gtagttcccc 4560
tcacagatct gcatctccca ggctttcatt tcagagggag ggatcatatc cacctgcggg 4620
gcgatgaaaa agacagtttc tggcgcaggg gagattaact gggatgagag caggtttctg 4680
agcagctgtg actttccaca gccggtgggc ccatatatca cgcctatcac cggctgcagc 4740
tggtagttaa gagagctgca gctgccgtcc tcccggagca ggggggccac ctcgttgagc 4800
atatccctga cgtggatgtt ctccctgacc agttccgcca gaaggcgctc gccgcccagc 4860
gaaagcagct cttgcaagga agcaaaattt ttcagcggtt tcaggccatc ggccgtgggc 4920
atgtttttca gcgtctgggt cagcagctcc agcctgtccc agagctcggt gatgtgctct 4980
acggcatctc gatccagcag atctcctcgt ttcgcgggtt ggggcggctt tcgctgtagg 5040
gcaccagccg atgggcgtcc agcggggcca gagtcatgtc cttccatggg cgcagagtcc 5100
tcgtcagggt ggtctgggtc acggtgaagg ggtgcgctcc gggttgggca ctggccaggg 5160
tgcgcttgag gctggttctg ctggtgctga atcgctgccg ctcttcgccc tgcgcgtcgg 5220
ccaggtagca tttgaccatg gtctcgtagt cgagaccctc ggcggcgtgc cccttggcgc 5280
ggagctttcc cttggaggtg gcgccgcacg aggggcactg caggctcttc agggcgtaga 5340
gcttgggagc gagaaacacg gactctgggg agtaggcgtc cgcgccgcag gccgcgcaga 5400
ccgtctcgca ttccaccagc caagtgagtt ccgggcggtc agggtcaaaa accaggttgc 5460
ccccatgctt tttgatgcgt ttcttacctc ggctctccat gaggcggtgt cccttctcgg 5520
tgacgaagag gctgtccgtg tccccgtaga ccgacttcag gggcctgtct tccagcggag 5580
tgcctctgtc ctcctcgtag agaaactctg accactctga gacgaaggcc cgtgtccagg 5640
ccaggacgaa ggaggccacg tgggaggggt agcggtcgtt gtccactagc gggtccacct 5700
tctccagggt gtgcagacac atgtccccct cctccgcgtc cagaaaagtg attggcttat 5760
aggtgtagga cacgtgaccg ggggttcccg acgggggggt ataaaagggg gtgggcgccc 5820
tttcatcttc actctcttcc gcatcgctgt ctgcgagagc cagctgctgg ggtaagtatt 5880
ccctttcaaa ggcgggcatg acctcagcgc tcaggttgtc agtttctaaa aatgaggagg 5940
atttgatgtt cacctgtccg gaagtgatac ctttgagggt acctgggtcc atctggtcag 6000
aaaacactat ttttttgttg tcaagcttgg tggcgaacga cccgtagagg gcgttggaga 6060
gcagcttggc aatggagcgc agggtctggt ttttgtcgcg gtcggctcgc tccttggccg 6120
cgatgttgag ttgcacgtat tcgcgggcca cgcacttcca ctcggggaag acggtggtgc 6180
gctcgtctgg gatcaggcgc accctccagc cgcggttgtg cagggtgacc atgtcgacgc 6240
tggtggcgac ctcaccgcgc agacgctcgt tggtccagca gaggcggccg cctttgcgcg 6300
agcagaaggg gggtaggggg tccagctggt cctcgtttgg ggggtccgcg tcgatggtaa 6360
agaccccggg gagcaggcgc gggtcaaagt agtcgatctt gcaagcttgc atgtccagag 6420
cccgctgcca ttcgcgggcg gcgagcgcgc gctcgtaggg gttgaggggc gggccccagg 6480
gcatggggtg ggtgagcgcg gaggcgtaca tgccgcagat gtcatacacg tacaggggtt 6540
ccctgaggat accgaggtag gtggggtagc agcgcccccc gcggatgctg gcgcgcacgt 6600
agtcatagag ctcgtgggag ggggccagca tgttgagccc aaggttggtg cgctgggggc 6660
gctcggcgcg gaagacgatc tgtctgaaga tggcatggga gttggaggag atggtgggtc 6720
gctggaagac gttgaagctt gcttcttgca agcccacgga gtccctgacg aaggaggcgt 6780
aggactcgcg cagcttgtgc accagctcgg cggtgacctg gacgtcgagc gcgcagtagt 6840
cgagggtctc gcggatgatg tcatacttat cctccccctt ctttttccac agctcgcggt 6900
tgaggacgaa ctcttcgcgg tctttccagt actcttggag gggaaacccg tccgtgtccg 6960
aacggtaaga gcctagcatg tagaactggt tgacggcctg gtaggggcag cagcccttct 7020
ccacgggcag cgcgtaggcc tgcgccgcct tgcggaggga ggtgtgggta agggcgaaag 7080
tgtccctgac catgactttg aggtattgat gtctgaagtc tgtgtcatcg cagccgccct 7140
gttcccacag ggtgtagtcc gtgcgctttt tggagcgcgg gttgggcagg gagaaggtga 7200
ggtcattgaa gaggatcttc cccgctcgag gcatgaagtt tctggtgatg cgaaagggcc 7260
ctgggaccga ggagcggttg ttgatgacct gggcggccag gacgatctcg tcaaagccgt 7320
ttatgttgtg gcccacgatg tagagctcca ggaagcgggg ctggcccttg atggagggga 7380
gctttttaag ttcctcgtag gtaagctcct cgggcgattc caggccgtgc tcctccaggg 7440
cccagtcttg caagtgaggg ttggccgcca ggaaggatcg ccagaggtcg cgggccatga 7500
gggtctgcag gcggtcgcgg aaggttctga actgccgccc tacggccatc ttttcggggg 7560
tgatgcagta gaaggtgagg gggtctttct cccaggggtc ccatctgagc tcttgggcga 7620
ggtcgcgcgc ggcggcgacc agagcctcgt cgccccccag tttcatgacc agcatgaagg 7680
gcacgagctg cttgccaaag gctcccatcc aagtgtaggt ctctacatcg taggtgacaa 7740
agaggcgctc cgtgcgagga tgagagccga tcgggaagaa ctggatctcc cgccaccagt 7800
tggaggattg gctgttgatg tggtgaaagt agaagtcccg tctgcgggcc gagcactcgt 7860
gctggctttt gtaaaagcga ccgcagtact ggcagcgctg cacgggttgt atatcttgca 7920
cgaggtgaac ctggcgacct ctgacgagga agcgcagcgg gaatctaagt cccccgcctg 7980
gggtcccgtg tggctggtgg tcttctactt tggttgtctg gccgccagca tctgtctcct 8040
ggagggcgat ggtggagcag accaccacgc cgcgagagcc gcaggtccag atctcggcgc 8100
tcggcgggcg gagtttgatg acgacatcgc gcacattgga gctgtccatg gtctccagct 8160
cccgcggcgg caggtcagcc gggagttcct ggaggttcac ctcgcagaga cgggtcaagg 8220
cgcggacagt gttgagatgg tatctgattt caaggggcgt gttggaggcg gagtcgatgg 8280
cttgcagaag gccgcagccc cggggggcca cgatggttcc ccgcggggcg cgaggggagg 8340
cggaagctgg gggtgtgttc agaagcggtg acgcgggcgg gcccccggag gtaggggggg 8400
ttccggcccc acaggcatgg gcggcagggg cacgtcttcg ccgcgcgcgg gcaggggctg 8460
gtgctggctc cggagagcgc ttgcgtgcgc gacgacgcga cggttggtgt cctgtatctg 8520
gcgcctctga gtgaagacca cgggtcccgt gaccttgaac ctgaaagaga gttcgacaga 8580
atcaatctcg gcatcgttga cagcggcctg gcgcaggatc tcctgcacgt cgcccgagtt 8640
gtcctggtag gcgatctctg ccatgaactg ctcgatctct tcctcctgga gatctcctcg 8700
tccggcgcgc tccacggtgg ccgccaggtc gttggagatg cgacccatga gctgcgagaa 8760
ggcgttgagg ccgccctcgt tccagacccg gctgtagacc acgcccccct cggcgtcgcg 8820
ggcgcgcatg accacctggg ccaggttgag ctccacgtgt cgcgtgaaga cggcgtagtt 8880
gcgcaggcgc tggaaaaggt agttcagggt ggtggcggtg tgttcggcga cgaagaagta 8940
catgacccag cgccgcaacg tggattcatt gatgtccccc aaggcctcca ggcgctccat 9000
ggcctcgtag aagtccacgg cgaagttgaa aaactgggag ttgcgagcgg acacggtcaa 9060
ctcctcctcc agaagacgga tgagctcggc gacagtgtcg cgcacctcgc gctcgaaggc 9120
cacggggggc gcttcttcct cttccacctc ttcttccatg attgcttctt cttcctcagc 9180
cgggacggga gggggcggcg gcgggggagg ggcgcggcgg cggcggcggc gcaccggcag 9240
gcggtcgatg aagcgctcga tcatctcccc ccgcatgcgg cgcatggtct cggtgacggc 9300
gcggccgttc tcccgggggc gcagctcaaa gacgccgcct ctcatctcgc cgcggggcgg 9360
gcggccgtga ggtagcgaga cggcgctgac tatgcatctt aacaattgct gtgtaggtac 9420
gccgccaagg gacctgattg agtccagatc caccggatcc gaaaaccttt ggaggaaagc 9480
gtctatccag tcgcagtcgc aaggtaggct gagcaccgtg gcgggcgggg gcgggtcggg 9540
agagttcctg gcggagatgc tgctgatgat gtaattaaag taggcggtct tgagaaggcg 9600
gatggtggac aggagcacca tgtctttggg tccggcctgt tggatgcgga ggcggtcggc 9660
catgccccag gcctcgttct gacaccggcg caggtctttg tagtagtctt gcatgagtct 9720
ttccaccggc acctcttctc cttcctcttc tccatctcgc cggtggtttc tcgcgccgcc 9780
catgcgcgtg accccaaagc ccctgagcgg ctgcagcagg gccaggtcgg cgaccacgcg 9840
ctcggccaag atggcctgct gtacctgagt gagggtcctc tcgaagtcat ccatgtccac 9900
gaagcggtgg taggcgcccg tgttgatggt gtaggtgcag ttggccatga cggaccagtt 9960
gacggtctgg tgtcccggct gcgagagctc cgtgtaccgc aggcgcgaga aggcgcggga 10020
atcgaacacg tagtcgttgc aagtccgcac cagatactgg tagcccacca ggaagtgcgg 10080
cggaggttgg cgatagaggg gccagcgctg ggtggcgggg gcgccgggcg ccaggtcttc 10140
cagcatgagg cggtggtatc cgtagatgta cctggacatc caggtgatgc cggcggcggt 10200
ggtggtggcg cgcgcgtagt cgcggacccg gttccagatg tttcgcaggg gcgagaagtg 10260
ttccatggtc ggcacgctct ggccggtgag gcgcgcgcag tcgttgacgc tctatacaca 10320
cacaaaaacg aaagcgttta cagggctttc gttctgtagc ctggaggaaa gtaaatgggt 10380
tgggttgcgg tgtgccccgg ttcgagacca agctgagctc ggccggctga agccgcagct 10440
aacgtggtat tggcagtccc gtctcgaccc aggccctgta tcctccagga tacggtcgag 10500
agcccttttg ctttcttggc caagcgcccg tggcgcgatc tgggatagat ggtcgcgatg 10560
agaggacaaa agcggctcgc ttccgtagtc tggagaaaca atcgccaggg ttgcgttgcg 10620
gcgtaccccg gttcgagccc ctatggcggc ttggatcggc cggaaccgcg gctaacgtgg 10680
gctgtggcag ccccgtcctc aggaccccgc cagccgactt ctccagttac gggagcgagc 10740
cccttttgtt ttttattttt tagatgcatc ccgtgctgcg gcagatgcgc ccctcgcccc 10800
ggcccgatca gcagcagcaa cagcaggcat gcagaccccc ctctcctcta cccgccccgg 10860
tcaccacggc cgcggcggcc gtgtccggcg cggggggcgc gctggagtca gatgagccac 10920
cgcggcggcg acctaggcag tatctggact tggaagaggg cgagggactg gcgcggctgg 10980
gggcgagctc tccagagcgc cacccgcggg tgcagttgaa aagggacgcg cgcgaggcgt 11040
acctgccgcg gcagaacctg tttcgcgacc gcgggggcga ggagcccgag gagatgcgag 11100
actgcaggtt ccaagcgggg cgcgagctgc gccgcgggtt ggacagacag cgcctgctgc 11160
gcgaggagga ctttgagccc gacacgcaga cgggcatcag ccccgcgcgc gcgcacgtgg 11220
ccgcggccga cctggtgacc gcctacgagc agacggtgaa ccaggagcgc aacttccaaa 11280
aaagcttcaa caaccacgtg cgcacgctgg tggcgcgcga ggaggtgacc ctgggtctca 11340
tgcatctgtg ggacctggtg gaggcgatcg tgcagaaccc cagcagcaag cccctgaccg 11400
cgcagctgtt cctggtggtg cagcacagca gggacaacga ggccttcagg gaggcgctgc 11460
tgaacatcac cgagccggag gggcgctggc tcctggacct gataaacatc ctgcagagca 11520
tagtggtgca ggagcgcagc ctgagcctgg ccgagaaggt ggcggccatt aactattcta 11580
tgctgagctt gggcaagttc tacgcccgca agatctacaa gaccccctac gtgcccatag 11640
acaaggaggt gaagatagac agcttctaca tgcgcatggc gctaaaggtg ctgaccctga 11700
gcgacgacct gggagtgtac cgcaacgagc gcatccacaa ggccgtgagc gccagccggc 11760
ggcgcgagct gagcgaccgc gagctgatgc acagtctgca gcgcgcgctc accggcgcgg 11820
gcgagggcga cagggaggtc gagtcctact tcgacatggg ggctgacctg cactggcagc 11880
cgagccgccg cgccctggag gcggcggggg cgtatggcgg ccccctggcg gccgatgacg 11940
aggaagagga ggactatgag ctagaggagg gcgagtacct ggaggactga cctggctggt 12000
ggtgttttgg tatagatgca agatccgaac gtggcggacc cggcggtccg ggcggcgctg 12060
cagagccagc cgtccggcat taactcctct gacgactggg ccgcggccat gggtcgcatc 12120
atggccctga ccgcgcgcaa ccccgaggcc ttcaggcagc agcctcaggc taaccggctg 12180
gcggccatct tggaagcggt agtgcccgcg cgctccaacc ccacccacga gaaggtgctg 12240
gccatagtca acgcgctggc ggagagcagg gccatccggg cggacgaggc cggactggtg 12300
tacgatgcgc tgctgcagcg ggtggcgcgg tacaacagcg gcaacgtgca gaccaacctg 12360
gaccgcctgg tgacggacgt gcgcgaggcc gtggcgcagc gcgagcgctt gcatcaggac 12420
ggtaacctgg gctcgctggt ggcgctaaac gccttcctca gcacccagcc ggccaacgta 12480
ccgcgggggc aggaggacta caccaacttc ttgagcgcgc tgcggctgat ggtgaccgag 12540
gtccctcaga gcgaggtgta ccagtcgggg cccgactact tcttccagac cagcagacag 12600
ggcttgcaaa ccgtgaacct gagccaggct ttcaagaacc tgcgggggct gtggggagtg 12660
aaagcgccca ccggcgaccg agctacggtg tccagcctgc taacccccaa ctcgcgcctg 12720
ctgctgctgc tgatcgcgcc cttcacggac agcgggagcg tctcgcggga gacctatctg 12780
ggccacctgc tgacgctgta ccgcgaggcc atcgggcagg cgcaggtgga cgagcacacc 12840
ttccaagaga tcaccagcgt gagccacgcg ctggggcagg aggacacggg cagcctgcag 12900
gcgaccctga actacctgct gaccaacagg cggcagaaga ttcccacgct gcacagcctg 12960
acccaggagg aggagcgcat cttgcgctac gtgcagcaga gcgtgagcct gaacctgatg 13020
cgcgacggcg tgacgcccag cgtggcgctg gacatgaccg cgcgcaacat ggaaccgggc 13080
atgtacgctt cccagcggcc gttcatcaac cgcctgatgg actacttgca tcgggcggcg 13140
gccgtgaacc ccgagtactt caccaatgcc attctgaatc cccactggat gccccctccg 13200
ggtttctaca acggagactt cgaggtgcct gaggtcaatg atgggttcct ctgggatgac 13260
atggatgaca gtgtgttctc ccccaacccg ctgcgcgccg cgtctctgcg attgaaggag 13320
ggctctgaca gggaaggacc gaggagtctg gcctcctccc tggctctggg ggcggtgggc 13380
gccacgggcg cggcggcgcg gggcagcagc cccttcccca gcctggcaga ctctctgaat 13440
agcgggcggg tgagcaggcc ccgcttgcta ggcgaggagg agtatctgaa caactccctg 13500
ctgcagcccg tgagggacaa aaacgctcag cgacagcagt ttcccaacaa cgggatagag 13560
agcctggtgg acaagatgtc cagatggaag acatatgcgc aggagtacaa ggagtgggag 13620
gaccgccagc cgcggcccct gccgccccct agacagcgct ggcagcggcg cgcgtccaac 13680
cgccgctgga gacagggacc cgaggacgat gatgactctg cagatgacag cagcgtgttg 13740
gacctgggcg ggagcgggaa ccccttttcg cacctgcgcc cacgcctggg caagatgttt 13800
taaaagagaa aaataaaact caccaaggcc atggcgacga gcgttggttt tttgttccct 13860
tccttagtat gcggcgcgcg gcgatgttcg aggaggggcc tcccccctct tacgagagcg 13920
cgatgggaat ttctcctgcg gcgcccctgc agcctcccta cgtgcctcct cggtacctgc 13980
aacctacagg ggggagaaat agcatctgtt actctgagct gcagcccctg tacgatacca 14040
ccagactgta cctggtggac aacaagtccg cggacgtggc ctccctgaac taccagaacg 14100
accacagcga ttttttgacc acggtgatcc aaaacaacga cttcacccca accgaggcca 14160
gtacccagac cataaacctg gacaacaggt cgaactgggg cggcgacctg aagaccatcc 14220
tgcacaccaa catgcccaac gtgaacgagt tcatgtttac caactctttt aaggcgcggg 14280
ttatggtggc gcgcgagcag ggggaggcga agtacgagtg ggtggacttc acgctgcccg 14340
agggcaacta ctcagagacc atgactattg acctgatgaa caatgcgatc gtggaacact 14400
acctgaaagt gggcaggcag aacggggtga aggagagcga tatcggggtc aagtttgaca 14460
ccagaaactt tcgtctgggc tgggaccccg tgaccgggct ggtcatgccg ggggtctaca 14520
ccaacgaggc ctttcatccc gatatagtgc tcctgcccgg ctgtggggtg gactttaccc 14580
agagccggct gagcaacctg ctgggcgttc gcaagcggca acctttccag gagggtttca 14640
agatcaccta tgaggatctg gaggggggca acattcccgc gctccttgat ctggacgcct 14700
acgaggagag cttgaaaccc gaggagagcg ctggcgacag cggcgagagt ggcgaggagc 14760
aagccggcgg cggtggcagc gcgtcggtag aaaacgaaag tactcccgca gtggcggcag 14820
acgctgcgga ggtcgagcca gaggccatgc agcaggacgc agaggagggc gcacaggagg 14880
gcgcgcagga ggacatgaac gatggggaga tcaggggaga cactttcgcc acccggggcg 14940
aagaaaaaga ggcagaggcg gtggcggcga cggtggaagc cgaaaccgag gcagaggcag 15000
agcccaagac cgaagttatg gaagacatga atgatggaga acgtaggggt gacacgtttg 15060
ccacccgggg cgaagagaag gcggcggagg cagaagccgc ggctgaggag gcggctgcgg 15120
ctgcggccga ggctgaggct gcggctgagg ctaaggtcga agccgatgtt gcggttgagg 15180
ctcaggctga ggaggaggag gcggcgactg aagcagttaa ggaaaaggcc caggcagagc 15240
aggaagagaa aaaacctgtc attcaacctc taaaagaaga tagcaaaaag cgcagttaca 15300
acgtcatcga gggcagcacc tttacccagt accgcagctg gtacctggcg tacaactacg 15360
gcgacccggt caagggggtg cgctcgtgga ccctgctctg cacgccggac gtcacctgcg 15420
gctccgagca gatgtattgg tcgctgccaa acatgatgca agacccggtg accttccgct 15480
ccacgcggca ggttagcaac ttcccggtgg tgggcgccga actgctgccc gtgcactcca 15540
agagttttta caacgagcag gccgtctact cccagctgat ccgccaggcc acctctctga 15600
cccacgtgtt caatcgcttt cccgagaacc agattttggc gcgcccgccg gcccccacca 15660
tcaccaccgt cagtgaaaac gttcctgccc tcacagatca cgggacgcta ccgctgcgca 15720
acagcatctc aggagtccag cgagtgacca ttactgacgc cagacgccgg acctgcccct 15780
acgtttacaa ggccttgggc atagtctcgc cgcgcgtcct ctccagtcgc actttttaaa 15840
atacatctac cctcacgctt caaaatcatg tccgtactca tctcacccag caacaacacc 15900
ggctgggggc tgcgcgcgcc cagcaagatg tttggagggg cgaggaaacg ctccgagcag 15960
cacccagtgc gcgtgcgcgg ccactaccgc gcgccctggg gagcgcacaa gcgcgggcgc 16020
gcagggcgca ccactgtgga cgacgccatt gactccgtag tggagcaggc gcgccactac 16080
acacccggcg cgccgtccgc ccccgccgtg tccaccgtgg acgaggcgat cgagagcgtg 16140
gtacagggcg cgcggcacta tgccaacctt aaaaatcgac gccgtcgcgt ggctcgccgc 16200
catcgccgga gaccccgggc caccgccgcc gcgcgccttg ctaaggctct gctcaggcgc 16260
gccaggcgaa ctggccgccg ggccgccatg agggccgcac ggcgggctgc cgccagcgcg 16320
gccgccgcgg ccccacgggc acgaaggcgc gcggccgctg ccgccgccgc cgccatttcc 16380
agcttggcct cgacgcggcg cggtaacata tactgggtgc gcgactcggt aaccggcacg 16440
cgggtacccg tgcgctttcg ccccccgcgg aattagcaca agacaacata cacactgagt 16500
ctcctgctgt tgtgtatccc agcggcgacc gtcagcagcg gcgacatgtc caagcgcaaa 16560
attaaagaag agatgctcca ggtcatcgcg ccggagatct atgggccccc gaagaaggag 16620
gaggatgatt acaagccccg caagctaaag cgggtcaaaa agaaaaagaa agatgatgac 16680
gttgacgagg cggtggagtt tgtccgccgc atggcgccca ggcgccccgt gcagtggaag 16740
ggtcggcgcg tgcagcgagt cctgcgcccc ggcaccgcgg tggtctttac gcccggcgag 16800
cgttccacgc gcactttcaa gcgggtgtac gatgaggtgt acggcgacga ggatctgttg 16860
gagcaggcca accatcgctt tggggagttt gcatatggga aacggccccg cgagagccta 16920
aaagaggacc tgctggcgct accgctggac gagggcaatc ccaccccgag tctgaagccg 16980
gtaaccctgc aacaggtgct gcctttgagc gcgcccagcg agcagaagcg agggttgaag 17040
cgcgagggcg gggacctggc acccaccgtg cagttgatgg tgcccaagcg gcagaagctg 17100
gaggacgtgc tggagaaaat gaaagtagag cccgggatcc agcccgaaat caaggtccgc 17160
cccatcaagc aggtggcgcc cggcgtggga gtccagaccg tggacgttag gattcccacg 17220
gaggagatgg aaacccaaac cgccactccc tcttcggcgg ctagcgccac caccggctcc 17280
gcttcggtag aggtgcagac ggacccctgg ctagccgccg ccgccccggc cgccccccgt 17340
tcgcgcgggc gcaagagaaa ttatccagcg gccagcgcgc tcatgcccca gtacgcactg 17400
catccatcca tcgcgcccac ccccggctac cgcgggtact cgtaccgccc gcgcagatca 17460
gccggcaccc gcggccgccg ccgccgtgcg accacaacca gccgccgccg tcgccgccgc 17520
cgccagccag tgctgacccc cgtgtctgta aggaaggtgg ctcgctcggg gagcacgctg 17580
gtggtgccca gagcgcgcta ccaccccagc attgtttaaa gccggtctct gtatggttct 17640
tgcagatatg gccctcactt gtcgcctccg cttcccggtg ccgggatacc gaggaagaac 17700
tcaccgccgc agaggcatgg cgggcagtgg tctccgcggc ggccgtcgcc atcgccggcg 17760
cgcaaagagc aggcgcatgc gcggcggtgt gctgcccttc ctaatcccgc taatcgccgc 17820
ggcgatcggt gccgtgcccg ggatcgcctc cgtggccctg caggcgtccc agaaacattg 17880
actcttgcaa ccttgcaagc ttgcattttt tggaggaaaa aataaaaagt ctagactctc 17940
acgctcgctt ggtcctgtga ctattttgta gaaaaaagat ggaagacatc aactttgcgt 18000
cgctggcccc gcgtcacggc tcgcgcccgt tcatgggaga ctggacagat atcggcacca 18060
gcaatatgag cggtggcgcc ttcagctggg gcagtctgtg gagtggcctt aaaaattttg 18120
gttccaccat taagaactat ggcaacaaag cgtggaacag cagcacgggt cagatgctga 18180
gagacaagtt gaaagagcag aacttccagg agaaggtggc acagggcctg gcctctggca 18240
tcagcggggt ggtggacata gctaaccagg ccgtgcagaa aaagataaac agtcatctgg 18300
acccccgccc tcaggtggag gaaacgcctc cagccatgga gacggtgtct cccgagggca 18360
aaggcgaaaa gcgcccgcgg cccgacaggg aagagaccct ggtgtcacac accgaggagc 18420
cgccctctta cgaggaggca gtcaaggccg gcctgcccac cactcgcccc atagctccca 18480
tggccaccgg tgtggtgggt cacaggcaac acacccccgc gacactagat ctgcccccgc 18540
cgtccgagcc gactcgccag ccaaaggcgg tgacggtgcc cgctccctcc acttccgccg 18600
ccaacagagt gcctctgcgc cgcgctgcga gcggcccccg ggcctcgcga gtcagcggca 18660
actggcagag cacactgaac agcatcgtgg gcctgggagt gaggagtgtg aagcgccgcc 18720
gttgctactg aatgagcaag ctagctaacg tgttgtatgt gtgtatgcgt cctatgtcgc 18780
cgccagagga gctgttgagc cgccggcgcc gtctgcactc cagcgaattt caagatggcg 18840
accccatcga tgatgcctca gtggtcgtac atgcacatct cgggccagga cgcttcggag 18900
tacctgagcc ccgggctggt gcagttcgcc cgcgccacag acacctactt caacatgagt 18960
aacaagttca ggaaccccac tgtggcgccc acccacgatg tgaccacgga ccggtcgcag 19020
cgcctgacgc tgcggttcat ccccgtggat cgggaggaca ccgcttactc ttacaaggcg 19080
cggttcacgc tggccgtggg cgacaaccgc gtgctggaca tggcctccac ttactttgac 19140
atccgggggg tgctggacag gggccccact ttcaagccct actcgggcac tgcctacaac 19200
tccctggccc ccaagggcgc tcccaattct tgcgagtggg aggaggaaac acaaaatgag 19260
gtacaagcca atgaagaaca actagcagaa gaagaggatg aagaaatggc tcaagaggat 19320
cagcagccta ctaaaaaaac ccatgtatat gctcaggcac ctctttctgg cgaacagatt 19380
accaaagatg gcttgcaaat aggagctgaa gttacaggag aaacatcaaa gcccattttt 19440
gcagacaaga cattccaacc agaacctcag ataggagagt ctcaatggaa tgaggccgat 19500
gctacagtag caggaggtag ggttttgaaa aagactaccc ctatgaaacc ttgctatgga 19560
tcctatgcca gacctaccaa tgccaatgga gggcagggga tacttgaggc aaatgctaaa 19620
ggggaactcg aatctaaagt tgagatgcag tttttctcta acaccacaac tcttaatgta 19680
agagacggtg aaaatggcct taaaccaaaa gtagtgctgt atagcgaaga tgtcaacctg 19740
gaatcccctg acactcatct gtcttacaag cccaaaaaag atgatgttaa tgccaaaatc 19800
atgttgggtc agcaagccat gcccaacaga cccaacctca ttggatttag agataatttc 19860
attgggctca tgtattacaa cagcactgga aacatgggag tgctggcggg tcaggcctct 19920
cagttgaatg ctgtggtgga cttgcaggat agaaacacgg aactgtcata tcagcttatg 19980
cttgattcca ttggagatag aaccagatac ttttccatgt ggaaccaggc agtggatagc 20040
tatgacccag atgttagaat cattgaaaac catggggtgg aggatgagct gcccaactac 20100
tgttttccct tgggcggtat aggaattaca gatacatacc aggccataaa agcagccaat 20160
ggtggagatg ctactacgtg gtctgctgat aacacatttg cagaccgcaa cgaaataggg 20220
gtgggaaaca acttcgccat ggagatcaac atccaggcca acctctggag aaacttcctc 20280
tatgcgaacg tgggactcta cctgccagac aagctcaagt acaaccccac caacgtggac 20340
atctctgaca accccaacac ctatgactac atgaacaagc gggtggtggc ccccggcctg 20400
gtggactgct ttgtcaatgt gggagccagg tggtccctgg actacatgga caacgtcaac 20460
cccttcaacc accaccgcaa tgcgggtctg cgctaccgct ccatgatcct gggcaacggg 20520
cgctatgtgc ccttccacat ccaggtaccc cagaagttct ttgccatcaa gaacctcctg 20580
ctcctgcccg gctcctacac ctacgagtgg aacttcagga aggatgtaaa catggtccta 20640
cagagctctc tgggcaatga ccttagggta gatggggcca gcatcaagtt tgacagcatc 20700
accctctatg ctacattttt ccccatggcc cacaacaccg cctccacgct tgaggccatg 20760
ctgagaaacg acaccaacga ccagtccttc aatgactacc tctctggggc caacatgctc 20820
tacccaatcc cagccaaggc caccaacgtg cccatctcca tcccctctcg caactgggcc 20880
gcctttagag gctgggcctt tacccgcctt aagaccaagg agaccccctc cctgggctcg 20940
ggttttgatc cctactttgt ttactcggga tccatcccct acctggatgg caccttctac 21000
ctcaaccaca ctttcaagaa gatatccatc atgtatgact cctccgtcag ctggccgggc 21060
aacgaccgct tgctcacccc caatgagttc gaggtcaagc gcgccgtgga cggcgagggc 21120
tacaacgtgg cccagtgcaa catgaccaag gactggttcc tggtgcagat gctggccaac 21180
tacaacatag gctaccaggg cttctacatc ccagagagct acaaggacag gatgtattcc 21240
ttcttcagaa atttccaacc catgagccga caggtggtgg acgagaccaa ttacaaggac 21300
tatcaggcca ttggcatcac ccaccagcac aacaactcgg gtttcgtggg ctacctggcg 21360
cccaccatgc gcgagggaca ggcctacccc gccaacttcc cctaccccct gataggcaag 21420
accgcggtcg acagcgtcac ccagaaaaag ttcctctgcg accgcaccct ctggcgcatc 21480
cccttctcta gcaactttat gtccatgggt gcgctcacgg acctgggcca aaacctgctt 21540
tatgccaact ctgcccatgc gctggacatg acttttgagg tggaccccat ggacgagccc 21600
acccttctct atattgtgtt tgaagtgttc gacgtggtca gagtgcacca gccgcaccgc 21660
ggtgtcatcg agaccgtgta cctgcgtacg cccttctcag ccggcaacgc caccacctaa 21720
ggagacagcg ccgccgcctg catgactggt tccaccgagc aagagctcag ggccatcgcc 21780
agagacctgg gatgcggacc ctattttttg ggcacctatg acaaacgctt cccgggtttt 21840
atctcccgag acaagctcgc ctgcgccatc gtcaacacgg ccgcgcgcga gaccgggggc 21900
gtgcactggc tggcctttgg ctgggacccg cgctctaaaa cttgctacct ctttgacccc 21960
tttggcttct ccgatcagcg cctcaggcag atttatgagt ttgagtacga ggggctgttg 22020
cgccgcagcg cgcttgcctc ctcgcccgac cgctgcatca cccttgagaa gtccaccgag 22080
accgtgcagg ggccccactc ggccgcctgc ggtctcttct gttgcatgtt tttgcacgcc 22140
tttgtgcact ggcctcagag tcccatggat cgcaacccca ccatgaactt gctaaaggga 22200
gtgcccaacg ccatgctcca gagcccccag gtcctgccca ccctgcgccg caaccaggaa 22260
cagctctacc gcttcctgga gcgccactcc ccctacttcc gcagccacag cgcgcgcatc 22320
cggggggcca cctctttttg ccacttgcaa gaaaacatgc aagacggaaa atgatgtaca 22380
gcatgctttt aataaatgta aagactgtgc actttattta tacacgggct ctttctggtt 22440
atttattcaa caccgccgtc gccatctaga aatcgaaagg gttctgccgc gcgtcgccgt 22500
gcgccacggg cagagacacg ttgcgatact ggaagcggct cgcccacttg aactcgggca 22560
ccaccatgcg gggcagtggt tcctcgggga aattctcgct ccacagggtg cgggtcagct 22620
gcagcgcgct caggaggtcg ggagccgaga tcttgaagtc gcagttgggg ccggaaccct 22680
gcgcgcgcga gttgcggtac acggggttgc agcactggaa caccagcagg gccggattat 22740
tcacgctggc cagcaggctc tcgtcgctaa tcatgtcgct gtccagatcc tccgcgttgc 22800
tcagggcgaa tggggtcatc ttgcagacct gcctgcccag gaaaggcggg agcccaggct 22860
tgccgttaca gtcgcagcgc aggggcatta gcaggtgccc acggcccgac tgcgcctgcg 22920
ggtacaacgc gcgcatgaag gcttcgatct gcctaaaagc cacctgggtc ttggctccct 22980
ccgaaaagaa catcccacag gacttgctgg agaactggtt cgcgggacag ctggcatcgt 23040
gcaggcagca gcgcgcgtca gtgttggcga tctgcaccac gttgcgaccc caccggtttt 23100
tcactatctt ggccttggaa gcctgctcct ttagcgcgcg ctggccgttc tcgctggtca 23160
catccatctc tatcacctgt tccttgttga tcatgtttgt cccgtgcaga cactttaggt 23220
cgccctccgt ctgggtgcag cggtgctccc acagcgcgca accggtgggc tcccaatttt 23280
tgtgggtcac ccccgcgtag gcctgcaggt aggcctgcag gaagcgcccc atcatggtca 23340
taaaggtctt ctggctcgta aaggtcagct gcaggccgcg atgctcttcg ttcagccagg 23400
tcttgcagat ggcggccagc gcctcggtct gctcgggcag catcttaaaa tttgtcttca 23460
ggtcgttatc cacgtggtac ttgtccatca tggcacgcgc cgcctccatg cccttctccc 23520
aggcggacac catgggcagg cttagggggt ttatcacttc cagcggcgag gacaccgtac 23580
tttcgatttc ttcttcctcc ccctcttccc ggcgcgcgcc cccgctgttg cgcgctctta 23640
ccgcctgcac caaggggtcg tcttcaggca agcgccgcac cgagcgcttg ccgcccttga 23700
cctgcttgat cagtaccggc gggttgctga agcccaccat ggtcagcgcc gcctgctctt 23760
cttcgtcttc gctgtctacc actatttctg gggaggggct tctccgctct gcggcaaagg 23820
cggcggatcg cttctttttt ttcttgggag ccgccgcgat ggagtccgcc acggcgaccg 23880
aggtcgaggg cgtggggctg ggggtgcgcg gcaccagggc ctcgtcgccc tcggactctt 23940
cctctgactc caggcggcgg cggagtcgct tctttggggg cgcgcgcgtt agcggcggcg 24000
gagacgggga cggggacggg gacgggacgc cctccacagg gggcggtctt cgcgcagacc 24060
cgcggccgcg ctcgggggtc ttctcgcgct ggtcttggtc ccgactggcc attgtatcct 24120
cctcctccta ggcagagaga cataaggagt ctatcatgca agtcgagaag gaggagagct 24180
taaccacccc ctctgagacc gccgtcgccg tcgcccccgc taccgccgac gcgcccgcca 24240
caccgagcga cacccccgcg gacccccccg ccgacgcacc cctgttcgag gaagcggccg 24300
tggagcagga cccgggcttt gtctcggcag aggaggattt gcaagaggag gaggataagg 24360
aggagaagcc ctcagtgcca aaagatcata aagagcaaga cgagcacgac gcagacgcac 24420
accagggtga agtcgggcgg ggggacggag ggcatggcgg cgccgactac ctagacgaag 24480
gaaacgacgt gctcttgaag cacctgcatc gtcagtgcgc cattgtctgc gacgctctgc 24540
aggagcgcag cgaggtgccc ctcagcgtgg cggaggtcag ccgcgcctac gagctcagcc 24600
tcttttcccc ccgggtgccc ccccgccgcc gcgaaaacgg cacatgcgag cccaacccgc 24660
gcctcaactt ctaccccgcc tttgtggtgc ccgaggtcct ggccacctat cacatcttct 24720
ttcaaaattg caagatcccc atctcgtgcc gcgccaaccg tagccgcgcc gataagatgc 24780
tggccctgcg ccagggcgac cacatacctg atatcgccgc tttggaagat gtgccaaaga 24840
tcttcgaggg tctgggtcgc aacgagaagc gggcagcaaa ctctctgcaa caggaaaaca 24900
gcgaaaatga gagtcacact ggagcgctgg tggagctgga gggcgacaac gcccgcctgg 24960
cggtgctcaa gcgcagcatc gaggtcaccc actttgccta ccccgcgctc aacctgcccc 25020
ccaaagtcat gaacgcggtc atggacgggc taatcatgcg ccgcggccgg ccccttgctc 25080
cagatgcaaa cttgcatgag gagaccgagg acggtcagcc cgtggtcagc gacgagcagc 25140
tgacgcgctg gctggaaacc gcggaccccg ccgaactgga ggagcggcgc aagatgatga 25200
tggccgcggt gctggtcacc gtagagctgg agtgtctgca gcgcttcttc ggcgaccccg 25260
agatgcagag aaaggtcgag gagaccctac actacacctt ccgccagggc tacgtgcgcc 25320
aggcttgcaa gatctccaac gtggagctca gcaacctggt gtcctacctg ggcatcttgc 25380
atgaaaaccg ccttgggcag agcgtgctac actccaccct gcgcggggag gcgcgccgcg 25440
actacgtgcg cgactgcgtt tacctcttcc tctgctacac ctggcagacg gccatggggg 25500
tctggcagca gtgcctggag gagcgcaacc tcaaggagct ggagaagctc ctgcagcgcg 25560
cgctcaaaga cctctggacg ggctacaacg agcgctcggt ggccgccgcg ctggccgacc 25620
tcatcttccc cgagcgcctg ctcaaaactc tccagcaggg gctgcccgac ttcaccagcc 25680
aaagcatgtt gcaaaatttt aggaacttta tcctggagcg ttctggcatc ctacccgcca 25740
cctgctgcgc cctgcccagc gactttgtcc ccctcgtgta ccgcgagtgc cccccgccgc 25800
tgtggggcca ctgctacctg ttccaactgg ccaactacct gtcctaccac gcggacctca 25860
tggaagactc cagcggcgag gggctcatgg agtgccactg ccgctgcaac ctctgcacgc 25920
cccaccgctc cctggtctgc aacacccaac tgctcagcga gagtcagatt atcggtacct 25980
tcgagctaca gggtccgtcc tcctcagacg agaagtccgc ggctccgggg ctaaaactca 26040
ctccggggct gtggacttcc gcctacctgc gcaaatttgt acctgaagac taccacgccc 26100
acgagatcag gttttacgag gaccaatccc gcccgcccaa ggcggagctg accgcctgcg 26160
tcatcaccca gggcgagatc ctaggccaat tgcaagccat ccaaaaagcc cgccaagagt 26220
ttttgctgag aaagggtcgg ggggtgtatc tggaccccca gtcgggtgag gagctcaacc 26280
cggttccccc gctgccgccg ccgcgggacc ttgcttccca ggataagcat cgccatggct 26340
cccagaaaga agcagcagcg gccgccactg ccgccacccc acacgctgga ggaagaggag 26400
gaatactggg acagtcaggc agaggaggtt tcggacgagg aggagccgga gacggagatg 26460
gaagagtggg aggaggacag cttagacgag gaggcttccg aagccgaaga ggcaggcgca 26520
acaccgtcac cctcggccgc agccccctcg caggcgcccc cgaagtccgc tcccagcatc 26580
agcagcaaca gcagcgctat aacctccgct cctccaccgc cgcgacccac ggccgaccgc 26640
agacccaacc gtagatggga caccaccgga accggggccg gtaagtcctc cgggaaaggc 26700
aagcaagcgc agcgccaagg ctaccgctcg tggcgcgctc acaagaacgc catagtcgct 26760
tgcttgcaag actgcggggg gaacatctcc ttcgcccgcc gcttcctgct cttccaccac 26820
ggtgtggcct tcccccgtaa cgtcctgcat tactaccgtc atctctacag cccctactgc 26880
ggcggcagtg agccagaggc ggccggcggc agcggcgccc gtttcggtgc ctaggaagac 26940
ccagggcaag acttcagcca agaaactcgc ggcggccgcg gcgaacgcgg tcgcgggggc 27000
cctgcgcctg acggtgaacg aacccctgtc gacccgcgaa ctgaggaacc gaatcttccc 27060
cactctctat gccatcttcc agcagagcag agggcaggat caggaactga aagtaaaaaa 27120
caggtctctg cgctccctca cccgcagctg tctgtatcac aagagcgaag accagcttcg 27180
gcgcacgctg gaggacgctg aggcactctt cagcaaatac tgcgcgctca ctcttaagga 27240
ctagctccgc gcccttctcg aatttaggcg ggaacgccta cgtcatcgca gcgccgccgt 27300
catgagcaag gacattccca cgccatacat gtggagctat cagccgcaga tgggactcgc 27360
ggcgggcgcc tcccaggatt actccacccg catgaactgg ctcagtgccg gcccacacat 27420
gatctcacag gttaatgaca tccgcaccca tcgaaaccaa atattggtgg agcaggcggc 27480
aattaccacc acgccccgca ataatcccaa ccccagggag tggcccgcgt ccctggtgta 27540
tcaggaaatt cccggcccca ccaccgtact acttccgcgt gattcccagg ccgaagtcca 27600
aatgactaac tcaggggcac agctcgcggg cggctgtcgt cacagggtgc ggcctcctcg 27660
ccagggtata actcacctga agatccgagg cagaggtatt cagctcaacg acgagtcggt 27720
gagctcctcg ctcggtctca gacctgacgg gaccttccag atagccggag ccggccgatc 27780
ttccttcacg ccccgccagg cgtacctgac tctgcagagc tcgtcctcgg cgccgcgctc 27840
gggcggcatc gggactctcc agttcgtgca ggagtttgtg ccctcggtct acttcaaccc 27900
cttctcgggc tctcccggtc gctacccgga ccagttcatc ccgaactttg acgccgcgag 27960
ggactcggtg gacggctacg actgaatgtc gggtggaccc ggtgcagagc aacttcgcct 28020
gaagcacctt gaccactgcc gccgccctca gtgctttgcc cgctgtcaga ccggtgagtt 28080
ccagtacttt tccctgcccg actcgcaccc ggacggcccg gcgcacgggg tgcgcttttt 28140
catcccgagt caggtccgct ctaccctaat cagggagttc accgcccgtc ccctactggc 28200
ggagttggaa aaggggcctt ctatcctaac cattgcctgc atctgctcta accctggatt 28260
acaccaagat ctttgctgtc atttgtgtgc tgagtataat aaaggctgag atcagaatct 28320
actcgggctc ctgtcgccat cctgtcaacg ccaccgtcca agcccggccc gatcagcccg 28380
aggtgaacct cacctgcggt ctgcaccggc gcctgaggaa atacctagct tggtactaca 28440
acagcactcc ctttgtggtt tacaacagct ttgaccagga cggggtctca ctgagggata 28500
acctctcgaa cctgagctac tccatcagga agaacaacac cctcgagcta cttcctcctt 28560
acctgcccgg gacttaccag tgtgtcaccg gtccctgcac ccacacccac ctgttgatcg 28620
taaacgactc tcttccgaga acagacctca ataactcctc tccgcagttc cccagaacag 28680
gaggtgagct caggaaaccc cgggtaaaga agggtggaca agagttaaca cttgtggggt 28740
ttctggtgta tgtgacgctg gtggtggctc ttttgattaa ggcttttcct tccatgtctg 28800
aactatccct cttcttttat gaacaactcg actagtgcta acgggaccct acccaacgaa 28860
tcgggattga atatcggtaa ccaggttgca gtttcacttt tgattacctt catagtcctc 28920
ttcctgctag tgctgtcgct tctgtgcctg cggatcgggg gctgctgcat ccacgtttat 28980
atctggtgct ggctgtttag aaggttcgga gaccaccgca ggtagaataa acaaacctag 29040
acctagaaat ggacggtctc tgcagcgagc aacgcatact agagaggcgc cggcaaaaag 29100
cagagctcga gcgtcttaaa caagagctcc aagacgccgt ggccatacac cagtgcaaaa 29160
aagggctctt ctgtctggta aaacaggcca cgctcaccta tgaaaaaaca ggtgacaccc 29220
accgcctagg atacaagctg cccacacagc gccaaaagtt tgcccttatg ataggtgaac 29280
aacccatcac cgtgacccag cactccgtgg agacagaagg ctgcattcat gctccctgca 29340
ggggcgctga ctgcctctac accttgatca aaaccctttg cggtctcaga gaccttatcc 29400
ctttcaattg atcataactg taatcaataa aaaatcactt acttgaaatc tgatagcaag 29460
cctctgtcca attttttcag caacacttcc ttcccctctt cccaactctg gtactctagg 29520
cgcctcctag ctgcaaactt cctccacagt ctgaagggaa tgtcagattc ctcctcctcc 29580
tgtccctccg cacccacgat cttcatgttg ttgcagatga agcgcaccaa aacgtctgac 29640
gagagcttca accccgtgta cccctatgac acggaaaacg gtcctccctc cgtccctttc 29700
ctcacccctc ccttcgtgtc tcccgatgga ttccaagaga gcccccccgg ggtcctgtct 29760
ctgaacctgg ccgagcccct ggtcacttcc cacggcatgc tcgccctgaa aatgggaagt 29820
ggcctctccc tggacgacgc cggcaacctc acctctcaag atgtcaccac cactacccct 29880
cccctgaaaa aaaccaagac caacctcagc ctagaaacct cagcccccct gactgtgagc 29940
acctcaggcg ccctcaccct agcagccgcc gttcccctgg cggtggccgg cacctccctc 30000
accatgcaat cagaggcccc cctgacagtc caagatgcaa aactcaccct ggccaccaag 30060
ggccccctga ccgtgtctga aggcaaactg gccttgcaga cctcggcccc gctgacggcc 30120
gctgacagca gcgccctcac cgttagcgcc acaccaccca tcagtgtaag cagtggaagt 30180
ttgggcttag acatggaaga ccccatgtat actcatgatg gaaaactggg aataagaatt 30240
ggaggcccac tgagagtagt agacagcctg cacacactga ctgtagttac cggaaatgga 30300
atagctgtag ataacaatgc cctccaaact agagttacgg gcgccctggg ttatgacaca 30360
tcaggaaacc tacaactgag agccgcgggg ggtatgcgaa ttgatgcaaa tggccaactt 30420
atccttgatg tggcataccc atttgatgct caaaacaatc tcagccttag acttggtcag 30480
ggacccctgt atgtaaacac agaccacaac ctagatttga attgcaacag aggtctgacc 30540
acaactacca ccaacaacac aaaaaaactt gaaactaaaa ttggctcagg cttagactat 30600
gataccaatg gtgctgtcat tattaaactt ggtactggtg taagctttga cagcacaggc 30660
gccctaactg tgggaaacac tggcgatgat aaactgactc tgtggacaac cccagaccca 30720
tctccaaatt gcagaattca cgcagacaaa gactgcaagt ttactctagt cctaactaag 30780
tgtggaagtc aaatcctggc ttctgtcgcc gccctagcgg tgtcaggaaa tctggcttca 30840
ataacaggca ccgttgccag cgttaccatc tttctcagat ttgatcagaa tggagtgctt 30900
atggaaaact cctccctaga caagcagtac tggaacttca gaaatggtaa ctcaaccaat 30960
gccaccccct acaccaatgc agttgggttc atgccaaacc tcgcagcata ccccaagaca 31020
cagagccaga ctgctaaaaa caacattgta agtcaggttt acttgaatgg ggacaaatcc 31080
aaacccatga cccttaccat taccctcaat ggaactaatg aatccagtga aactagccag 31140
gtgagtcact actccatgtc atttacatgg gcttgggaga gtgggcaata tgccaccgaa 31200
acctttgcca ccaattcctt taccttctct tacattgctg aacaataaag aaagcacaga 31260
gatgcttgtt tttgatttca aaattgtgtg cttttattta ttttcaagct tacagtattt 31320
ccagtagtca ttcgaataga gcttaatgaa actgcatgag aacccttcca catagcttaa 31380
attatcacca gtgcaaatgg agaaaaatca acataccttt ttatccagat atcacagaac 31440
cctagtattc aacctgccac ctccctccca acacacagag tacacagtcc tttctccccg 31500
gctggcctta aaaagcatca tatcatgggt aacagacata ttcttaggtg ttatattcca 31560
cacggtttcc tgtcgagcca aacgctcatc agtgatatta ataaactccc cgggcagctc 31620
acttaagttc atgtcgctgt ccagctgctg agccacaggc tgctgtccaa cttgcggttg 31680
cttaacgggc ggcgaaggag aagtccacgc ctacatgggg gtagagtcat aatcgtgcat 31740
caggataggg cggtggtgct gcagcagcgc gcgaataaac tgctgccgcc gccgctccgt 31800
cctgcaggaa tacaacatgg cagtggtctc ctcagcgatg attcgcaccg cccgcagcat 31860
aaggcgcctt gtcctccggg cacagcagcg caccctgatc tcacttaaat cagcacagta 31920
actgcagcac agcaccacaa tattgttcaa aatcccacag tgcaaggcgc tgtatccaaa 31980
gctcatggcg gggaccacag aacccacgtg gccatcatac cacaagcgca ggtagattaa 32040
gtggcgaccc ctcataaaca cgctggacat aaacattacc tcttttggca tgttgtaatt 32100
caccacctcc cggtaccata taaacctctg attaaacatg gcgccatcca ccaccatcct 32160
aaaccagctg gccaaaacct gcccgccggc tatacactgc agggaaccgg gactggaaca 32220
atgacagtgg agagcccagg actcgtaacc atggatcatc atgctcgtca tgatatcaat 32280
gttggcacaa cacaggcaca cgtgcataca cttcctcagg attacaagct cctcccgcgt 32340
tagaaccata tcccagggaa caacccattc ctgaatcagc gtaaatccca cactgcaggg 32400
aagacctcgc acgtaactca cgttgtgcat tgtcaaagtg ttacattcgg gcagcagcgg 32460
atgatcctcc agtatggtag cgcgggtttc tgtctcaaaa ggaggtagac gatccctact 32520
gtacggagtg cgccgagaca accgagatcg tgttggtcgt agtgtcatgc caaatggaac 32580
gccggacgta gtcatatttc ctccagcaga accaagtgcg cgcgtggcag ctatccttgc 32640
gtcttctgtc tcgccgcctg ccccgctcgg tgtagtagtt gtaatacagc cactccctca 32700
gaccgtcaag gcgctccctg gcgtccggat ctataacaac accatcctgc agcgccgccc 32760
tgatgacatc caccaccgta gagtatgcca agcccagcca ggaaatgcac tcactttgac 32820
agcgagagat aggaggagcg ggaagagatg gaagaaccat gatagtaaaa gaacttttat 32880
tccaatcgat cctctacaat gtcaaagtgt agatctatca gatggcactg gtctcctccg 32940
ctgagtcgat caaaaataac agctaaacca caaacaacac gattggtcaa atgctgcaca 33000
agggcttgca gcataaaatc gcctcgaaag tccaccgcaa gcataacatc aaagccaccg 33060
cccctatcat gatctatgat aaaaacccca cagctatcca ccagacccat atagttttca 33120
tctctccatc gtgaaaaaat atttacaagc tcctccttta aatcacctcc aaccaattca 33180
aaaagttgag ccagaccgcc ctccaccttc attttcagca tgcgcatcat gattgcaaaa 33240
attcaggctc ctcagacacc tgtataagat tgagaagcgg aacattaaca tcaatgtttc 33300
gctcgcgaag atcgcgcctc agtgcaagca tgatataatc ccacaggtcg gagcggatca 33360
gcgaggacat ctccccgcca ggaaccaact caacggagcc tatgctgatt ataatacgca 33420
tattcggggc tatgctaacc agcacggccc ccaaataggc gtactgcata ggcggcgaca 33480
aaaagtgaac agtttgggtt aaaaaatcag gcaaacactc gcgcaaaaaa gcaagaacat 33540
cataaccatg ctcatgcaaa tagatgcaag taagctcagg aacgaccaca gaaaaatgca 33600
caatttttct ctcaaacatg actgcgagcc ctgcaaaaaa taaaaaagaa acattacaca 33660
agagtagcct gtcttacaat gggatagact actctaacca acataagacg ggccacgaca 33720
tcgcccgcgt ggccataaaa aaaattatcc gtgtgattaa aaagaagcac agatagctgg 33780
ccagtcatat ccggagtcat cacgtgcgaa cccgtgtaga cccccgggtt ggacacatcg 33840
gccaaagaaa gaaagcggcc aatgtatccc ggaggaatga taacactaag acgaagatac 33900
aacagaataa ccccatgggg gggaataaca aagttagtag gtgaataaaa acgataaaca 33960
cccgaaactc cctcctgcgt aggcaaaata gcgccctccc cttccaaaac aacatacagc 34020
gcttccacag cagccatgac aaaagactca aaacactcaa aagactcagt cttaccagga 34080
aaataaaagc actctcacag caccagcact aatcagagtg tgaagagggc caagtgccga 34140
acgagtatat ataggaatta aaaatgacgt aaatgtgtaa aggtcaaaaa acgcccagaa 34200
aaatacacag accaacgccc gaaacgaaaa cccgcgaaaa aatacccaga agttcctcaa 34260
caaccgccac ttccgctttc ccacgatacg tcacttcctc gaaaatagca aactacattt 34320
cccacatgta caaaaccgaa accactcccc ttgtcaccgc ccacaactta catcttaatt 34380
aacaaacgtc aaagcctacg tcagccgccc cgcctcgccc cgcccacctc attatcatat 34440
tggccacaat ccaaaataag gtatattatt gatgatgatt taaatattat gcggtgtgaa 34500
ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc 34560
actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 34620
gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 34680
cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 34740
ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 34800
ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 34860
ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 34920
agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 34980
cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 35040
aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 35100
gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 35160
agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 35220
ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 35280
cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 35340
tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 35400
aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 35460
tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg 35520
atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata 35580
cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg 35640
gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct 35700
gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt 35760
tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc 35820
tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 35880
tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 35940
aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 36000
atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 36060
tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca 36120
catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 36180
aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct 36240
tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc 36300
gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa 36360
tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt 36420
tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc 36480
taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt 36540
cgtcttcaag aattgattta aat 36563
<210> 16
<211> 37555
<212> DNA
<213> 人工序列
<220>
<223> pBZ1_BZ28F.RSVF-2A-GLuc
<400> 16
catcatcaat aatatacctt attttggatt gtggccaata tgataatgag gtgggcgggg 60
cgaggcgggg cgggtgacgt aggacgtgcg agtagggttg ggaggtgtgg cggaagtgtg 120
gcatttgtaa gtgggaggag cttacatgca agcttccgtc gcggaaaatg tgacgttttt 180
tatgagcgcc gcctacctcc ggaagtgcca attttcgcgc gctttttacc ggatatcgta 240
gtaattttgg gcgggaccat gtaagatttg gccattttcg cgcgaaaagt gaaacgggga 300
agtgaaaact gaataatagg gcgttagtca tagcgcgtaa tatttaccga gggccgaggg 360
actttgaccg attacgtgga ggactcgccc aggtgttttt tacgtgaatt tccgcgttcc 420
gggtcaaagt ctccgttttt attgtcaccg tcatttgacg cggagggtat ttaaacccgc 480
tgcgctccta gcgatcgctc aatattggcc attagccata ttattcattg gttatatagc 540
ataaatcaat attggctatt ggccattgca tacgttgtat ccatatcata atatgtacat 600
ttatattggc tcatgtccaa cattaccgcc atgttgacat tgattattga ctagttatta 660
atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata 720
acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat 780
aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga 840
gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc 900
ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt 960
atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat 1020
gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag 1080
tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc 1140
aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga 1200
ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg 1260
ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggtt ggccgtgctc 1320
ttcctgacgg gtaggtgtcc cctaacctag ggagccaacc atcggggggc cttctcccta 1380
aatccccgtg gcccaccctc ctgggcagag gcagcaggtt tctcactggc cccctctccc 1440
ccacctccaa gcttggcctt tcggctcaga tctcagccca cagctggcct gatctgggtc 1500
tcccctccca ccctcaggga gccaggctcg gcatttcgtc gacatggaac tgctgatcct 1560
gaaggccaac gccatcacca ccatcctgac cgccgtgacc ttctgcttcg ccagcggcca 1620
gaacatcacc gaggaattct accagagcac ctgtagcgcc gtgtccaagg gctacctgag 1680
cgccctgcgg accggctggt acaccagcgt gatcaccatc gagctgagca acatcaaaaa 1740
gaacaagtgc aacggcaccg acgccaaaat caagctgatc aagcaggaac tggacaagta 1800
caagaacgcc gtgaccgagc tgcagctgct gatgcagagc acccccgcca ccaacaaccg 1860
ggccagacgg gagctgcccc ggttcatgaa ctacaccctg aacaacgcca aaaagaccaa 1920
cgtgaccctg agcaagaagc ggaagcggcg gttcctgggc ttcctgctgg gcgtgggcag 1980
cgccattgct agcggagtgg ctgtgtctaa ggtgctgcac ctggaaggcg aagtgaacaa 2040
gatcaagtcc gccctgctga gcaccaacaa ggccgtggtg tccctgagca acggcgtgtc 2100
cgtgctgacc agcaaggtgc tggatctgaa gaactacatc gacaagcagc tgctgcccat 2160
cgtgaacaag cagagctgca gcatcagcaa catcgagaca gtgatcgagt tccagcagaa 2220
gaacaaccgg ctgctggaaa tcacccgcga gttcagcgtg aacgccggcg tgaccacccc 2280
cgtgtccacc tacatgctga ccaacagcga gctgctgagc ctgatcaacg acatgcccat 2340
caccaacgac cagaaaaagc tgatgagcaa caacgtgcag atcgtgcggc agcagagcta 2400
ctccatcatg tccatcatca aagaagaggt gctggcctac gtggtgcagc tgcccctgta 2460
cggcgtgatc gacaccccct gctggaagct gcacaccagc cccctgtgca ccaccaacac 2520
caaagagggc agcaacatct gcctgacccg gaccgaccgg ggctggtact gcgataatgc 2580
cggcagcgtg tcattctttc cacaagccga gacatgcaag gtgcagagca accgggtgtt 2640
ctgcgacacc atgaacagcc tgaccctgcc cagcgaggtg aacctgtgca acgtggacat 2700
cttcaaccct aagtacgact gcaagatcat gacctccaag accgacgtgt ccagctccgt 2760
gatcacctcc ctgggcgcca tcgtgtcctg ctacggcaag accaagtgca ccgccagcaa 2820
caagaaccgg ggcatcatca agaccttcag caacggctgc gactacgtgt ccaacaaggg 2880
cgtggacacc gtgtccgtgg gcaacaccct gtactacgtg aacaaacagg aaggcaagag 2940
cctgtacgtg aagggcgagc ccatcatcaa cttctacgac cccctggtgt tccccagcga 3000
cgagttcgac gccagcatca gccaggtcaa cgagaagatc aaccagagcc tggccttcat 3060
cagaaagagc gacgagctgc tgcacaatgt gaatgccgtg aagtccacca ccaatatcat 3120
gatcaccaca atcatcatcg tgatcatcgt catcctgctg tccctgatcg ccgtgggcct 3180
gctgctgtac tgcaaggccc ggtccacccc tgtgaccctg tccaaggacc agctgagcgg 3240
catcaacaat atcgccttct ccaacggacg cgtgaccgag ctgctttacc ggatgaagcg 3300
ggctgagaca tattgcccga gacccctgtt ggcaatccat cctactgagg ctcgccacaa 3360
acagaaaatc gtggcccccg tcaaacagac actcaatttt gacttgttga aacttgcagg 3420
agatgttgag tcaaaccccg ggcctatggg cgtcaaggtc ctgttcgctc tgatttgtat 3480
cgctgtcgct gaagctaagc caaccgagaa taatgaagac tttaatatcg tggccgtggc 3540
ttctaacttc gctaccacag acctggatgc agacagggga aagctgccag gcaagaaact 3600
gcccctggag gtcctgaagg agatggaagc aaatgcccgg aaagccgggt gcacaagagg 3660
atgcctgatt tgtctgagcc acatcaagtg cactcctaag atgaagaagt tcatccccgg 3720
ccggtgccat acctacgagg gcgataagga atccgcccag ggaggaatcg gagaggctat 3780
cgtggatatt cccgaaatcc ctggcttcaa agacctggag cccatggaac agtttattgc 3840
acaggtggat ctgtgcgtcg actgtactac cggatgcctg aagggactgg caaacgtcca 3900
gtgtagcgac ctgctgaaga aatggctgcc tcagcgatgt gctacatttg ccagcaagat 3960
tcagggccag gtggacaaga ttaagggagc aggaggcgac tgataattct agacgagatc 4020
cgaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 4080
caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 4140
cttatcatgt ctgcgatcgc tgatgagacc aggaccaggt gccgaccctg cgagtgcggc 4200
ggcaagcaca tgagaaatca gcctgtgatg ttggatgtga ccgaggagct taggcctgac 4260
catctggtgc tggcctgcac cagggccgag tttgggtcta gcgatgagga taccgattga 4320
ggtgggtaag gtgggcgtgg ctagcagggt gggcgtgtat aaattggggg tctaaggggt 4380
ctctctgttt gtcttgcaac agccgccgcc atgagcgaca ccggcaacag ctttgatgga 4440
agcatcttta gcccctatct gacagtgcgc atgcctcact gggccggagt gcgtcagaat 4500
gtgatgggtt ccaacgtgga tggacgtccc gttctgcctt caaattcgtc tacgatggcc 4560
tacgcgaccg tgggaggaac tccgctggac gccgcgacct ccgccgccgc ctccgccgcc 4620
gccgcgaccg cgcgcagcat ggctacggac ctttacagct ctttggtggc gagcagcgcg 4680
gcctctcgcg cgtctgctcg ggatgaaaaa ctgactgctc tgctgcttaa actggaagac 4740
ttgacccggg agctgggtca actgacccag caggtctcca gcttgcgtga gagcagcctt 4800
gcctccccct aatggcccat aatataaata aaagccagtc tgtttggatt aagcaagtgt 4860
atgttcttta tttaactctc cgcgcgcggt aagcccggga ccagcggtct cggtcgttta 4920
gggtgcggtg gattctttcc aacacgtggt acaggtggct ctggatgttt agatacatgg 4980
gcatgagtcc atccctgggg tggaggtagc accactgcag agcttcgtgc tcgggggtgg 5040
tgttgtatat gatccagtcg tagcaggagc gctgggcgtg gtgctgaaaa atgtccttaa 5100
gcaagaggct tatagctagg gggaggccct tggtgtaagt gtttacaaat ctgcttagct 5160
gggaggggtg catccggggg gatatgatgt gcatcttgga ctggattttt aggttggcta 5220
tgttcccacc cagatccctt ctgggattca tgttgtgcag gaccaccagc acggtatatc 5280
cagtgcactt gggaaattta tcgtggagct tagacgggaa tgcatggaag aacttggaga 5340
cgcccttgtg gcctcccaga ttttccatac attcgtccat gatgatggca atgggcccgt 5400
gggaagctgc ctgagcaaaa acgtttctgg catcgctcac atcgtagtta tgttccaggg 5460
tgaggtcatc ataggacatc tttacaaatc gggggcggag ggtcccggac tgggggatga 5520
tggtaccctc gggccccggg gcgtagttcc cctcacagat ctgcatctcc caggctttca 5580
tttcagaggg agggatcata tccacctgcg gggcgatgaa aaagacagtt tctggcgcag 5640
gggagattaa ctgggatgag agcaggtttc tgagcagctg tgactttcca cagccggtgg 5700
gcccatatat cacgcctatc accggctgca gctggtagtt aagagagctg cagctgccgt 5760
cctcccggag caggggggcc acctcgttga gcatatccct gacgtggatg ttctccctga 5820
ccagttccgc cagaaggcgc tcgccgccca gcgaaagcag ctcttgcaag gaagcaaaat 5880
ttttcagcgg tttcaggcca tcggccgtgg gcatgttttt cagcgtctgg gtcagcagct 5940
ccagcctgtc ccagagctcg gtgatgtgct ctacggcatc tcgatccagc agatctcctc 6000
gtttcgcggg ttggggcggc tttcgctgta gggcaccagc cgatgggcgt ccagcggggc 6060
cagagtcatg tccttccatg ggcgcagagt cctcgtcagg gtggtctggg tcacggtgaa 6120
ggggtgcgct ccgggttggg cactggccag ggtgcgcttg aggctggttc tgctggtgct 6180
gaatcgctgc cgctcttcgc cctgcgcgtc ggccaggtag catttgacca tggtctcgta 6240
gtcgagaccc tcggcggcgt gccccttggc gcggagcttt cccttggagg tggcgccgca 6300
cgaggggcac tgcaggctct tcagggcgta gagcttggga gcgagaaaca cggactctgg 6360
ggagtaggcg tccgcgccgc aggccgcgca gaccgtctcg cattccacca gccaagtgag 6420
ttccgggcgg tcagggtcaa aaaccaggtt gcccccatgc tttttgatgc gtttcttacc 6480
tcggctctcc atgaggcggt gtcccttctc ggtgacgaag aggctgtccg tgtccccgta 6540
gaccgacttc aggggcctgt cttccagcgg agtgcctctg tcctcctcgt agagaaactc 6600
tgaccactct gagacgaagg cccgtgtcca ggccaggacg aaggaggcca cgtgggaggg 6660
gtagcggtcg ttgtccacta gcgggtccac cttctccagg gtgtgcagac acatgtcccc 6720
ctcctccgcg tccagaaaag tgattggctt ataggtgtag gacacgtgac cgggggttcc 6780
cgacgggggg gtataaaagg gggtgggcgc cctttcatct tcactctctt ccgcatcgct 6840
gtctgcgaga gccagctgct ggggtaagta ttccctttca aaggcgggca tgacctcagc 6900
gctcaggttg tcagtttcta aaaatgagga ggatttgatg ttcacctgtc cggaagtgat 6960
acctttgagg gtacctgggt ccatctggtc agaaaacact atttttttgt tgtcaagctt 7020
ggtggcgaac gacccgtaga gggcgttgga gagcagcttg gcaatggagc gcagggtctg 7080
gtttttgtcg cggtcggctc gctccttggc cgcgatgttg agttgcacgt attcgcgggc 7140
cacgcacttc cactcgggga agacggtggt gcgctcgtct gggatcaggc gcaccctcca 7200
gccgcggttg tgcagggtga ccatgtcgac gctggtggcg acctcaccgc gcagacgctc 7260
gttggtccag cagaggcggc cgcctttgcg cgagcagaag gggggtaggg ggtccagctg 7320
gtcctcgttt ggggggtccg cgtcgatggt aaagaccccg gggagcaggc gcgggtcaaa 7380
gtagtcgatc ttgcaagctt gcatgtccag agcccgctgc cattcgcggg cggcgagcgc 7440
gcgctcgtag gggttgaggg gcgggcccca gggcatgggg tgggtgagcg cggaggcgta 7500
catgccgcag atgtcataca cgtacagggg ttccctgagg ataccgaggt aggtggggta 7560
gcagcgcccc ccgcggatgc tggcgcgcac gtagtcatag agctcgtggg agggggccag 7620
catgttgagc ccaaggttgg tgcgctgggg gcgctcggcg cggaagacga tctgtctgaa 7680
gatggcatgg gagttggagg agatggtggg tcgctggaag acgttgaagc ttgcttcttg 7740
caagcccacg gagtccctga cgaaggaggc gtaggactcg cgcagcttgt gcaccagctc 7800
ggcggtgacc tggacgtcga gcgcgcagta gtcgagggtc tcgcggatga tgtcatactt 7860
atcctccccc ttctttttcc acagctcgcg gttgaggacg aactcttcgc ggtctttcca 7920
gtactcttgg aggggaaacc cgtccgtgtc cgaacggtaa gagcctagca tgtagaactg 7980
gttgacggcc tggtaggggc agcagccctt ctccacgggc agcgcgtagg cctgcgccgc 8040
cttgcggagg gaggtgtggg taagggcgaa agtgtccctg accatgactt tgaggtattg 8100
atgtctgaag tctgtgtcat cgcagccgcc ctgttcccac agggtgtagt ccgtgcgctt 8160
tttggagcgc gggttgggca gggagaaggt gaggtcattg aagaggatct tccccgctcg 8220
aggcatgaag tttctggtga tgcgaaaggg ccctgggacc gaggagcggt tgttgatgac 8280
ctgggcggcc aggacgatct cgtcaaagcc gtttatgttg tggcccacga tgtagagctc 8340
caggaagcgg ggctggccct tgatggaggg gagcttttta agttcctcgt aggtaagctc 8400
ctcgggcgat tccaggccgt gctcctccag ggcccagtct tgcaagtgag ggttggccgc 8460
caggaaggat cgccagaggt cgcgggccat gagggtctgc aggcggtcgc ggaaggttct 8520
gaactgccgc cctacggcca tcttttcggg ggtgatgcag tagaaggtga gggggtcttt 8580
ctcccagggg tcccatctga gctcttgggc gaggtcgcgc gcggcggcga ccagagcctc 8640
gtcgcccccc agtttcatga ccagcatgaa gggcacgagc tgcttgccaa aggctcccat 8700
ccaagtgtag gtctctacat cgtaggtgac aaagaggcgc tccgtgcgag gatgagagcc 8760
gatcgggaag aactggatct cccgccacca gttggaggat tggctgttga tgtggtgaaa 8820
gtagaagtcc cgtctgcggg ccgagcactc gtgctggctt ttgtaaaagc gaccgcagta 8880
ctggcagcgc tgcacgggtt gtatatcttg cacgaggtga acctggcgac ctctgacgag 8940
gaagcgcagc gggaatctaa gtcccccgcc tggggtcccg tgtggctggt ggtcttctac 9000
tttggttgtc tggccgccag catctgtctc ctggagggcg atggtggagc agaccaccac 9060
gccgcgagag ccgcaggtcc agatctcggc gctcggcggg cggagtttga tgacgacatc 9120
gcgcacattg gagctgtcca tggtctccag ctcccgcggc ggcaggtcag ccgggagttc 9180
ctggaggttc acctcgcaga gacgggtcaa ggcgcggaca gtgttgagat ggtatctgat 9240
ttcaaggggc gtgttggagg cggagtcgat ggcttgcaga aggccgcagc cccggggggc 9300
cacgatggtt ccccgcgggg cgcgagggga ggcggaagct gggggtgtgt tcagaagcgg 9360
tgacgcgggc gggcccccgg aggtaggggg ggttccggcc ccacaggcat gggcggcagg 9420
ggcacgtctt cgccgcgcgc gggcaggggc tggtgctggc tccggagagc gcttgcgtgc 9480
gcgacgacgc gacggttggt gtcctgtatc tggcgcctct gagtgaagac cacgggtccc 9540
gtgaccttga acctgaaaga gagttcgaca gaatcaatct cggcatcgtt gacagcggcc 9600
tggcgcagga tctcctgcac gtcgcccgag ttgtcctggt aggcgatctc tgccatgaac 9660
tgctcgatct cttcctcctg gagatctcct cgtccggcgc gctccacggt ggccgccagg 9720
tcgttggaga tgcgacccat gagctgcgag aaggcgttga ggccgccctc gttccagacc 9780
cggctgtaga ccacgccccc ctcggcgtcg cgggcgcgca tgaccacctg ggccaggttg 9840
agctccacgt gtcgcgtgaa gacggcgtag ttgcgcaggc gctggaaaag gtagttcagg 9900
gtggtggcgg tgtgttcggc gacgaagaag tacatgaccc agcgccgcaa cgtggattca 9960
ttgatgtccc ccaaggcctc caggcgctcc atggcctcgt agaagtccac ggcgaagttg 10020
aaaaactggg agttgcgagc ggacacggtc aactcctcct ccagaagacg gatgagctcg 10080
gcgacagtgt cgcgcacctc gcgctcgaag gccacggggg gcgcttcttc ctcttccacc 10140
tcttcttcca tgattgcttc ttcttcctca gccgggacgg gagggggcgg cggcggggga 10200
ggggcgcggc ggcggcggcg gcgcaccggc aggcggtcga tgaagcgctc gatcatctcc 10260
ccccgcatgc ggcgcatggt ctcggtgacg gcgcggccgt tctcccgggg gcgcagctca 10320
aagacgccgc ctctcatctc gccgcggggc gggcggccgt gaggtagcga gacggcgctg 10380
actatgcatc ttaacaattg ctgtgtaggt acgccgccaa gggacctgat tgagtccaga 10440
tccaccggat ccgaaaacct ttggaggaaa gcgtctatcc agtcgcagtc gcaaggtagg 10500
ctgagcaccg tggcgggcgg gggcgggtcg ggagagttcc tggcggagat gctgctgatg 10560
atgtaattaa agtaggcggt cttgagaagg cggatggtgg acaggagcac catgtctttg 10620
ggtccggcct gttggatgcg gaggcggtcg gccatgcccc aggcctcgtt ctgacaccgg 10680
cgcaggtctt tgtagtagtc ttgcatgagt ctttccaccg gcacctcttc tccttcctct 10740
tctccatctc gccggtggtt tctcgcgccg cccatgcgcg tgaccccaaa gcccctgagc 10800
ggctgcagca gggccaggtc ggcgaccacg cgctcggcca agatggcctg ctgtacctga 10860
gtgagggtcc tctcgaagtc atccatgtcc acgaagcggt ggtaggcgcc cgtgttgatg 10920
gtgtaggtgc agttggccat gacggaccag ttgacggtct ggtgtcccgg ctgcgagagc 10980
tccgtgtacc gcaggcgcga gaaggcgcgg gaatcgaaca cgtagtcgtt gcaagtccgc 11040
accagatact ggtagcccac caggaagtgc ggcggaggtt ggcgatagag gggccagcgc 11100
tgggtggcgg gggcgccggg cgccaggtct tccagcatga ggcggtggta tccgtagatg 11160
tacctggaca tccaggtgat gccggcggcg gtggtggtgg cgcgcgcgta gtcgcggacc 11220
cggttccaga tgtttcgcag gggcgagaag tgttccatgg tcggcacgct ctggccggtg 11280
aggcgcgcgc agtcgttgac gctctataca cacacaaaaa cgaaagcgtt tacagggctt 11340
tcgttctgta gcctggagga aagtaaatgg gttgggttgc ggtgtgcccc ggttcgagac 11400
caagctgagc tcggccggct gaagccgcag ctaacgtggt attggcagtc ccgtctcgac 11460
ccaggccctg tatcctccag gatacggtcg agagcccttt tgctttcttg gccaagcgcc 11520
cgtggcgcga tctgggatag atggtcgcga tgagaggaca aaagcggctc gcttccgtag 11580
tctggagaaa caatcgccag ggttgcgttg cggcgtaccc cggttcgagc ccctatggcg 11640
gcttggatcg gccggaaccg cggctaacgt gggctgtggc agccccgtcc tcaggacccc 11700
gccagccgac ttctccagtt acgggagcga gccccttttg ttttttattt tttagatgca 11760
tcccgtgctg cggcagatgc gcccctcgcc ccggcccgat cagcagcagc aacagcaggc 11820
atgcagaccc ccctctcctc tacccgcccc ggtcaccacg gccgcggcgg ccgtgtccgg 11880
cgcggggggc gcgctggagt cagatgagcc accgcggcgg cgacctaggc agtatctgga 11940
cttggaagag ggcgagggac tggcgcggct gggggcgagc tctccagagc gccacccgcg 12000
ggtgcagttg aaaagggacg cgcgcgaggc gtacctgccg cggcagaacc tgtttcgcga 12060
ccgcgggggc gaggagcccg aggagatgcg agactgcagg ttccaagcgg ggcgcgagct 12120
gcgccgcggg ttggacagac agcgcctgct gcgcgaggag gactttgagc ccgacacgca 12180
gacgggcatc agccccgcgc gcgcgcacgt ggccgcggcc gacctggtga ccgcctacga 12240
gcagacggtg aaccaggagc gcaacttcca aaaaagcttc aacaaccacg tgcgcacgct 12300
ggtggcgcgc gaggaggtga ccctgggtct catgcatctg tgggacctgg tggaggcgat 12360
cgtgcagaac cccagcagca agcccctgac cgcgcagctg ttcctggtgg tgcagcacag 12420
cagggacaac gaggccttca gggaggcgct gctgaacatc accgagccgg aggggcgctg 12480
gctcctggac ctgataaaca tcctgcagag catagtggtg caggagcgca gcctgagcct 12540
ggccgagaag gtggcggcca ttaactattc tatgctgagc ttgggcaagt tctacgcccg 12600
caagatctac aagaccccct acgtgcccat agacaaggag gtgaagatag acagcttcta 12660
catgcgcatg gcgctaaagg tgctgaccct gagcgacgac ctgggagtgt accgcaacga 12720
gcgcatccac aaggccgtga gcgccagccg gcggcgcgag ctgagcgacc gcgagctgat 12780
gcacagtctg cagcgcgcgc tcaccggcgc gggcgagggc gacagggagg tcgagtccta 12840
cttcgacatg ggggctgacc tgcactggca gccgagccgc cgcgccctgg aggcggcggg 12900
ggcgtatggc ggccccctgg cggccgatga cgaggaagag gaggactatg agctagagga 12960
gggcgagtac ctggaggact gacctggctg gtggtgtttt ggtatagatg caagatccga 13020
acgtggcgga cccggcggtc cgggcggcgc tgcagagcca gccgtccggc attaactcct 13080
ctgacgactg ggccgcggcc atgggtcgca tcatggccct gaccgcgcgc aaccccgagg 13140
ccttcaggca gcagcctcag gctaaccggc tggcggccat cttggaagcg gtagtgcccg 13200
cgcgctccaa ccccacccac gagaaggtgc tggccatagt caacgcgctg gcggagagca 13260
gggccatccg ggcggacgag gccggactgg tgtacgatgc gctgctgcag cgggtggcgc 13320
ggtacaacag cggcaacgtg cagaccaacc tggaccgcct ggtgacggac gtgcgcgagg 13380
ccgtggcgca gcgcgagcgc ttgcatcagg acggtaacct gggctcgctg gtggcgctaa 13440
acgccttcct cagcacccag ccggccaacg taccgcgggg gcaggaggac tacaccaact 13500
tcttgagcgc gctgcggctg atggtgaccg aggtccctca gagcgaggtg taccagtcgg 13560
ggcccgacta cttcttccag accagcagac agggcttgca aaccgtgaac ctgagccagg 13620
ctttcaagaa cctgcggggg ctgtggggag tgaaagcgcc caccggcgac cgagctacgg 13680
tgtccagcct gctaaccccc aactcgcgcc tgctgctgct gctgatcgcg cccttcacgg 13740
acagcgggag cgtctcgcgg gagacctatc tgggccacct gctgacgctg taccgcgagg 13800
ccatcgggca ggcgcaggtg gacgagcaca ccttccaaga gatcaccagc gtgagccacg 13860
cgctggggca ggaggacacg ggcagcctgc aggcgaccct gaactacctg ctgaccaaca 13920
ggcggcagaa gattcccacg ctgcacagcc tgacccagga ggaggagcgc atcttgcgct 13980
acgtgcagca gagcgtgagc ctgaacctga tgcgcgacgg cgtgacgccc agcgtggcgc 14040
tggacatgac cgcgcgcaac atggaaccgg gcatgtacgc ttcccagcgg ccgttcatca 14100
accgcctgat ggactacttg catcgggcgg cggccgtgaa ccccgagtac ttcaccaatg 14160
ccattctgaa tccccactgg atgccccctc cgggtttcta caacggagac ttcgaggtgc 14220
ctgaggtcaa tgatgggttc ctctgggatg acatggatga cagtgtgttc tcccccaacc 14280
cgctgcgcgc cgcgtctctg cgattgaagg agggctctga cagggaagga ccgaggagtc 14340
tggcctcctc cctggctctg ggggcggtgg gcgccacggg cgcggcggcg cggggcagca 14400
gccccttccc cagcctggca gactctctga atagcgggcg ggtgagcagg ccccgcttgc 14460
taggcgagga ggagtatctg aacaactccc tgctgcagcc cgtgagggac aaaaacgctc 14520
agcgacagca gtttcccaac aacgggatag agagcctggt ggacaagatg tccagatgga 14580
agacatatgc gcaggagtac aaggagtggg aggaccgcca gccgcggccc ctgccgcccc 14640
ctagacagcg ctggcagcgg cgcgcgtcca accgccgctg gagacaggga cccgaggacg 14700
atgatgactc tgcagatgac agcagcgtgt tggacctggg cgggagcggg aacccctttt 14760
cgcacctgcg cccacgcctg ggcaagatgt tttaaaagag aaaaataaaa ctcaccaagg 14820
ccatggcgac gagcgttggt tttttgttcc cttccttagt atgcggcgcg cggcgatgtt 14880
cgaggagggg cctcccccct cttacgagag cgcgatggga atttctcctg cggcgcccct 14940
gcagcctccc tacgtgcctc ctcggtacct gcaacctaca ggggggagaa atagcatctg 15000
ttactctgag ctgcagcccc tgtacgatac caccagactg tacctggtgg acaacaagtc 15060
cgcggacgtg gcctccctga actaccagaa cgaccacagc gattttttga ccacggtgat 15120
ccaaaacaac gacttcaccc caaccgaggc cagtacccag accataaacc tggacaacag 15180
gtcgaactgg ggcggcgacc tgaagaccat cctgcacacc aacatgccca acgtgaacga 15240
gttcatgttt accaactctt ttaaggcgcg ggttatggtg gcgcgcgagc agggggaggc 15300
gaagtacgag tgggtggact tcacgctgcc cgagggcaac tactcagaga ccatgactat 15360
tgacctgatg aacaatgcga tcgtggaaca ctacctgaaa gtgggcaggc agaacggggt 15420
gaaggagagc gatatcgggg tcaagtttga caccagaaac tttcgtctgg gctgggaccc 15480
cgtgaccggg ctggtcatgc cgggggtcta caccaacgag gcctttcatc ccgatatagt 15540
gctcctgccc ggctgtgggg tggactttac ccagagccgg ctgagcaacc tgctgggcgt 15600
tcgcaagcgg caacctttcc aggagggttt caagatcacc tatgaggatc tggagggggg 15660
caacattccc gcgctccttg atctggacgc ctacgaggag agcttgaaac ccgaggagag 15720
cgctggcgac agcggcgaga gtggcgagga gcaagccggc ggcggtggca gcgcgtcggt 15780
agaaaacgaa agtactcccg cagtggcggc agacgctgcg gaggtcgagc cagaggccat 15840
gcagcaggac gcagaggagg gcgcacagga gggcgcgcag gaggacatga acgatgggga 15900
gatcagggga gacactttcg ccacccgggg cgaagaaaaa gaggcagagg cggtggcggc 15960
gacggtggaa gccgaaaccg aggcagaggc agagcccaag accgaagtta tggaagacat 16020
gaatgatgga gaacgtaggg gtgacacgtt tgccacccgg ggcgaagaga aggcggcgga 16080
ggcagaagcc gcggctgagg aggcggctgc ggctgcggcc gaggctgagg ctgcggctga 16140
ggctaaggtc gaagccgatg ttgcggttga ggctcaggct gaggaggagg aggcggcgac 16200
tgaagcagtt aaggaaaagg cccaggcaga gcaggaagag aaaaaacctg tcattcaacc 16260
tctaaaagaa gatagcaaaa agcgcagtta caacgtcatc gagggcagca cctttaccca 16320
gtaccgcagc tggtacctgg cgtacaacta cggcgacccg gtcaaggggg tgcgctcgtg 16380
gaccctgctc tgcacgccgg acgtcacctg cggctccgag cagatgtatt ggtcgctgcc 16440
aaacatgatg caagacccgg tgaccttccg ctccacgcgg caggttagca acttcccggt 16500
ggtgggcgcc gaactgctgc ccgtgcactc caagagtttt tacaacgagc aggccgtcta 16560
ctcccagctg atccgccagg ccacctctct gacccacgtg ttcaatcgct ttcccgagaa 16620
ccagattttg gcgcgcccgc cggcccccac catcaccacc gtcagtgaaa acgttcctgc 16680
cctcacagat cacgggacgc taccgctgcg caacagcatc tcaggagtcc agcgagtgac 16740
cattactgac gccagacgcc ggacctgccc ctacgtttac aaggccttgg gcatagtctc 16800
gccgcgcgtc ctctccagtc gcacttttta aaatacatct accctcacgc ttcaaaatca 16860
tgtccgtact catctcaccc agcaacaaca ccggctgggg gctgcgcgcg cccagcaaga 16920
tgtttggagg ggcgaggaaa cgctccgagc agcacccagt gcgcgtgcgc ggccactacc 16980
gcgcgccctg gggagcgcac aagcgcgggc gcgcagggcg caccactgtg gacgacgcca 17040
ttgactccgt agtggagcag gcgcgccact acacacccgg cgcgccgtcc gcccccgccg 17100
tgtccaccgt ggacgaggcg atcgagagcg tggtacaggg cgcgcggcac tatgccaacc 17160
ttaaaaatcg acgccgtcgc gtggctcgcc gccatcgccg gagaccccgg gccaccgccg 17220
ccgcgcgcct tgctaaggct ctgctcaggc gcgccaggcg aactggccgc cgggccgcca 17280
tgagggccgc acggcgggct gccgccagcg cggccgccgc ggccccacgg gcacgaaggc 17340
gcgcggccgc tgccgccgcc gccgccattt ccagcttggc ctcgacgcgg cgcggtaaca 17400
tatactgggt gcgcgactcg gtaaccggca cgcgggtacc cgtgcgcttt cgccccccgc 17460
ggaattagca caagacaaca tacacactga gtctcctgct gttgtgtatc ccagcggcga 17520
ccgtcagcag cggcgacatg tccaagcgca aaattaaaga agagatgctc caggtcatcg 17580
cgccggagat ctatgggccc ccgaagaagg aggaggatga ttacaagccc cgcaagctaa 17640
agcgggtcaa aaagaaaaag aaagatgatg acgttgacga ggcggtggag tttgtccgcc 17700
gcatggcgcc caggcgcccc gtgcagtgga agggtcggcg cgtgcagcga gtcctgcgcc 17760
ccggcaccgc ggtggtcttt acgcccggcg agcgttccac gcgcactttc aagcgggtgt 17820
acgatgaggt gtacggcgac gaggatctgt tggagcaggc caaccatcgc tttggggagt 17880
ttgcatatgg gaaacggccc cgcgagagcc taaaagagga cctgctggcg ctaccgctgg 17940
acgagggcaa tcccaccccg agtctgaagc cggtaaccct gcaacaggtg ctgcctttga 18000
gcgcgcccag cgagcagaag cgagggttga agcgcgaggg cggggacctg gcacccaccg 18060
tgcagttgat ggtgcccaag cggcagaagc tggaggacgt gctggagaaa atgaaagtag 18120
agcccgggat ccagcccgaa atcaaggtcc gccccatcaa gcaggtggcg cccggcgtgg 18180
gagtccagac cgtggacgtt aggattccca cggaggagat ggaaacccaa accgccactc 18240
cctcttcggc ggctagcgcc accaccggct ccgcttcggt agaggtgcag acggacccct 18300
ggctagccgc cgccgccccg gccgcccccc gttcgcgcgg gcgcaagaga aattatccag 18360
cggccagcgc gctcatgccc cagtacgcac tgcatccatc catcgcgccc acccccggct 18420
accgcgggta ctcgtaccgc ccgcgcagat cagccggcac ccgcggccgc cgccgccgtg 18480
cgaccacaac cagccgccgc cgtcgccgcc gccgccagcc agtgctgacc cccgtgtctg 18540
taaggaaggt ggctcgctcg gggagcacgc tggtggtgcc cagagcgcgc taccacccca 18600
gcattgttta aagccggtct ctgtatggtt cttgcagata tggccctcac ttgtcgcctc 18660
cgcttcccgg tgccgggata ccgaggaaga actcaccgcc gcagaggcat ggcgggcagt 18720
ggtctccgcg gcggccgtcg ccatcgccgg cgcgcaaaga gcaggcgcat gcgcggcggt 18780
gtgctgccct tcctaatccc gctaatcgcc gcggcgatcg gtgccgtgcc cgggatcgcc 18840
tccgtggccc tgcaggcgtc ccagaaacat tgactcttgc aaccttgcaa gcttgcattt 18900
tttggaggaa aaaataaaaa gtctagactc tcacgctcgc ttggtcctgt gactattttg 18960
tagaaaaaag atggaagaca tcaactttgc gtcgctggcc ccgcgtcacg gctcgcgccc 19020
gttcatggga gactggacag atatcggcac cagcaatatg agcggtggcg ccttcagctg 19080
gggcagtctg tggagtggcc ttaaaaattt tggttccacc attaagaact atggcaacaa 19140
agcgtggaac agcagcacgg gtcagatgct gagagacaag ttgaaagagc agaacttcca 19200
ggagaaggtg gcacagggcc tggcctctgg catcagcggg gtggtggaca tagctaacca 19260
ggccgtgcag aaaaagataa acagtcatct ggacccccgc cctcaggtgg aggaaacgcc 19320
tccagccatg gagacggtgt ctcccgaggg caaaggcgaa aagcgcccgc ggcccgacag 19380
ggaagagacc ctggtgtcac acaccgagga gccgccctct tacgaggagg cagtcaaggc 19440
cggcctgccc accactcgcc ccatagctcc catggccacc ggtgtggtgg gtcacaggca 19500
acacaccccc gcgacactag atctgccccc gccgtccgag ccgactcgcc agccaaaggc 19560
ggtgacggtg cccgctccct ccacttccgc cgccaacaga gtgcctctgc gccgcgctgc 19620
gagcggcccc cgggcctcgc gagtcagcgg caactggcag agcacactga acagcatcgt 19680
gggcctggga gtgaggagtg tgaagcgccg ccgttgctac tgaatgagca agctagctaa 19740
cgtgttgtat gtgtgtatgc gtcctatgtc gccgccagag gagctgttga gccgccggcg 19800
ccgtctgcac tccagcgaat ttcaagatgg cgaccccatc gatgatgcct cagtggtcgt 19860
acatgcacat ctcgggccag gacgcttcgg agtacctgag ccccgggctg gtgcagttcg 19920
cccgcgccac agacacctac ttcaacatga gtaacaagtt caggaacccc actgtggcgc 19980
ccacccacga tgtgaccacg gaccggtcgc agcgcctgac gctgcggttc atccccgtgg 20040
atcgggagga caccgcttac tcttacaagg cgcggttcac gctggccgtg ggcgacaacc 20100
gcgtgctgga catggcctcc acttactttg acatccgggg ggtgctggac aggggcccca 20160
ctttcaagcc ctactcgggc actgcctaca actccctggc ccccaagggc gctcccaatt 20220
cttgcgagtg ggaggaggaa acacaaaatg aggtacaagc caatgaagaa caactagcag 20280
aagaagagga tgaagaaatg gctcaagagg atcagcagcc tactaaaaaa acccatgtat 20340
atgctcaggc acctctttct ggcgaacaga ttaccaaaga tggcttgcaa ataggagctg 20400
aagttacagg agaaacatca aagcccattt ttgcagacaa gacattccaa ccagaacctc 20460
agataggaga gtctcaatgg aatgaggccg atgctacagt agcaggaggt agggttttga 20520
aaaagactac ccctatgaaa ccttgctatg gatcctatgc cagacctacc aatgccaatg 20580
gagggcaggg gatacttgag gcaaatgcta aaggggaact cgaatctaaa gttgagatgc 20640
agtttttctc taacaccaca actcttaatg taagagacgg tgaaaatggc cttaaaccaa 20700
aagtagtgct gtatagcgaa gatgtcaacc tggaatcccc tgacactcat ctgtcttaca 20760
agcccaaaaa agatgatgtt aatgccaaaa tcatgttggg tcagcaagcc atgcccaaca 20820
gacccaacct cattggattt agagataatt tcattgggct catgtattac aacagcactg 20880
gaaacatggg agtgctggcg ggtcaggcct ctcagttgaa tgctgtggtg gacttgcagg 20940
atagaaacac ggaactgtca tatcagctta tgcttgattc cattggagat agaaccagat 21000
acttttccat gtggaaccag gcagtggata gctatgaccc agatgttaga atcattgaaa 21060
accatggggt ggaggatgag ctgcccaact actgttttcc cttgggcggt ataggaatta 21120
cagatacata ccaggccata aaagcagcca atggtggaga tgctactacg tggtctgctg 21180
ataacacatt tgcagaccgc aacgaaatag gggtgggaaa caacttcgcc atggagatca 21240
acatccaggc caacctctgg agaaacttcc tctatgcgaa cgtgggactc tacctgccag 21300
acaagctcaa gtacaacccc accaacgtgg acatctctga caaccccaac acctatgact 21360
acatgaacaa gcgggtggtg gcccccggcc tggtggactg ctttgtcaat gtgggagcca 21420
ggtggtccct ggactacatg gacaacgtca accccttcaa ccaccaccgc aatgcgggtc 21480
tgcgctaccg ctccatgatc ctgggcaacg ggcgctatgt gcccttccac atccaggtac 21540
cccagaagtt ctttgccatc aagaacctcc tgctcctgcc cggctcctac acctacgagt 21600
ggaacttcag gaaggatgta aacatggtcc tacagagctc tctgggcaat gaccttaggg 21660
tagatggggc cagcatcaag tttgacagca tcaccctcta tgctacattt ttccccatgg 21720
cccacaacac cgcctccacg cttgaggcca tgctgagaaa cgacaccaac gaccagtcct 21780
tcaatgacta cctctctggg gccaacatgc tctacccaat cccagccaag gccaccaacg 21840
tgcccatctc catcccctct cgcaactggg ccgcctttag aggctgggcc tttacccgcc 21900
ttaagaccaa ggagaccccc tccctgggct cgggttttga tccctacttt gtttactcgg 21960
gatccatccc ctacctggat ggcaccttct acctcaacca cactttcaag aagatatcca 22020
tcatgtatga ctcctccgtc agctggccgg gcaacgaccg cttgctcacc cccaatgagt 22080
tcgaggtcaa gcgcgccgtg gacggcgagg gctacaacgt ggcccagtgc aacatgacca 22140
aggactggtt cctggtgcag atgctggcca actacaacat aggctaccag ggcttctaca 22200
tcccagagag ctacaaggac aggatgtatt ccttcttcag aaatttccaa cccatgagcc 22260
gacaggtggt ggacgagacc aattacaagg actatcaggc cattggcatc acccaccagc 22320
acaacaactc gggtttcgtg ggctacctgg cgcccaccat gcgcgaggga caggcctacc 22380
ccgccaactt cccctacccc ctgataggca agaccgcggt cgacagcgtc acccagaaaa 22440
agttcctctg cgaccgcacc ctctggcgca tccccttctc tagcaacttt atgtccatgg 22500
gtgcgctcac ggacctgggc caaaacctgc tttatgccaa ctctgcccat gcgctggaca 22560
tgacttttga ggtggacccc atggacgagc ccacccttct ctatattgtg tttgaagtgt 22620
tcgacgtggt cagagtgcac cagccgcacc gcggtgtcat cgagaccgtg tacctgcgta 22680
cgcccttctc agccggcaac gccaccacct aaggagacag cgccgccgcc tgcatgactg 22740
gttccaccga gcaagagctc agggccatcg ccagagacct gggatgcgga ccctattttt 22800
tgggcaccta tgacaaacgc ttcccgggtt ttatctcccg agacaagctc gcctgcgcca 22860
tcgtcaacac ggccgcgcgc gagaccgggg gcgtgcactg gctggccttt ggctgggacc 22920
cgcgctctaa aacttgctac ctctttgacc cctttggctt ctccgatcag cgcctcaggc 22980
agatttatga gtttgagtac gaggggctgt tgcgccgcag cgcgcttgcc tcctcgcccg 23040
accgctgcat cacccttgag aagtccaccg agaccgtgca ggggccccac tcggccgcct 23100
gcggtctctt ctgttgcatg tttttgcacg cctttgtgca ctggcctcag agtcccatgg 23160
atcgcaaccc caccatgaac ttgctaaagg gagtgcccaa cgccatgctc cagagccccc 23220
aggtcctgcc caccctgcgc cgcaaccagg aacagctcta ccgcttcctg gagcgccact 23280
ccccctactt ccgcagccac agcgcgcgca tccggggggc cacctctttt tgccacttgc 23340
aagaaaacat gcaagacgga aaatgatgta cagcatgctt ttaataaatg taaagactgt 23400
gcactttatt tatacacggg ctctttctgg ttatttattc aacaccgccg tcgccatcta 23460
gaaatcgaaa gggttctgcc gcgcgtcgcc gtgcgccacg ggcagagaca cgttgcgata 23520
ctggaagcgg ctcgcccact tgaactcggg caccaccatg cggggcagtg gttcctcggg 23580
gaaattctcg ctccacaggg tgcgggtcag ctgcagcgcg ctcaggaggt cgggagccga 23640
gatcttgaag tcgcagttgg ggccggaacc ctgcgcgcgc gagttgcggt acacggggtt 23700
gcagcactgg aacaccagca gggccggatt attcacgctg gccagcaggc tctcgtcgct 23760
aatcatgtcg ctgtccagat cctccgcgtt gctcagggcg aatggggtca tcttgcagac 23820
ctgcctgccc aggaaaggcg ggagcccagg cttgccgtta cagtcgcagc gcaggggcat 23880
tagcaggtgc ccacggcccg actgcgcctg cgggtacaac gcgcgcatga aggcttcgat 23940
ctgcctaaaa gccacctggg tcttggctcc ctccgaaaag aacatcccac aggacttgct 24000
ggagaactgg ttcgcgggac agctggcatc gtgcaggcag cagcgcgcgt cagtgttggc 24060
gatctgcacc acgttgcgac cccaccggtt tttcactatc ttggccttgg aagcctgctc 24120
ctttagcgcg cgctggccgt tctcgctggt cacatccatc tctatcacct gttccttgtt 24180
gatcatgttt gtcccgtgca gacactttag gtcgccctcc gtctgggtgc agcggtgctc 24240
ccacagcgcg caaccggtgg gctcccaatt tttgtgggtc acccccgcgt aggcctgcag 24300
gtaggcctgc aggaagcgcc ccatcatggt cataaaggtc ttctggctcg taaaggtcag 24360
ctgcaggccg cgatgctctt cgttcagcca ggtcttgcag atggcggcca gcgcctcggt 24420
ctgctcgggc agcatcttaa aatttgtctt caggtcgtta tccacgtggt acttgtccat 24480
catggcacgc gccgcctcca tgcccttctc ccaggcggac accatgggca ggcttagggg 24540
gtttatcact tccagcggcg aggacaccgt actttcgatt tcttcttcct ccccctcttc 24600
ccggcgcgcg cccccgctgt tgcgcgctct taccgcctgc accaaggggt cgtcttcagg 24660
caagcgccgc accgagcgct tgccgccctt gacctgcttg atcagtaccg gcgggttgct 24720
gaagcccacc atggtcagcg ccgcctgctc ttcttcgtct tcgctgtcta ccactatttc 24780
tggggagggg cttctccgct ctgcggcaaa ggcggcggat cgcttctttt ttttcttggg 24840
agccgccgcg atggagtccg ccacggcgac cgaggtcgag ggcgtggggc tgggggtgcg 24900
cggcaccagg gcctcgtcgc cctcggactc ttcctctgac tccaggcggc ggcggagtcg 24960
cttctttggg ggcgcgcgcg ttagcggcgg cggagacggg gacggggacg gggacgggac 25020
gccctccaca gggggcggtc ttcgcgcaga cccgcggccg cgctcggggg tcttctcgcg 25080
ctggtcttgg tcccgactgg ccattgtatc ctcctcctcc taggcagaga gacataagga 25140
gtctatcatg caagtcgaga aggaggagag cttaaccacc ccctctgaga ccgccgtcgc 25200
cgtcgccccc gctaccgccg acgcgcccgc cacaccgagc gacacccccg cggacccccc 25260
cgccgacgca cccctgttcg aggaagcggc cgtggagcag gacccgggct ttgtctcggc 25320
agaggaggat ttgcaagagg aggaggataa ggaggagaag ccctcagtgc caaaagatca 25380
taaagagcaa gacgagcacg acgcagacgc acaccagggt gaagtcgggc ggggggacgg 25440
agggcatggc ggcgccgact acctagacga aggaaacgac gtgctcttga agcacctgca 25500
tcgtcagtgc gccattgtct gcgacgctct gcaggagcgc agcgaggtgc ccctcagcgt 25560
ggcggaggtc agccgcgcct acgagctcag cctcttttcc ccccgggtgc ccccccgccg 25620
ccgcgaaaac ggcacatgcg agcccaaccc gcgcctcaac ttctaccccg cctttgtggt 25680
gcccgaggtc ctggccacct atcacatctt ctttcaaaat tgcaagatcc ccatctcgtg 25740
ccgcgccaac cgtagccgcg ccgataagat gctggccctg cgccagggcg accacatacc 25800
tgatatcgcc gctttggaag atgtgccaaa gatcttcgag ggtctgggtc gcaacgagaa 25860
gcgggcagca aactctctgc aacaggaaaa cagcgaaaat gagagtcaca ctggagcgct 25920
ggtggagctg gagggcgaca acgcccgcct ggcggtgctc aagcgcagca tcgaggtcac 25980
ccactttgcc taccccgcgc tcaacctgcc ccccaaagtc atgaacgcgg tcatggacgg 26040
gctaatcatg cgccgcggcc ggccccttgc tccagatgca aacttgcatg aggagaccga 26100
ggacggtcag cccgtggtca gcgacgagca gctgacgcgc tggctggaaa ccgcggaccc 26160
cgccgaactg gaggagcggc gcaagatgat gatggccgcg gtgctggtca ccgtagagct 26220
ggagtgtctg cagcgcttct tcggcgaccc cgagatgcag agaaaggtcg aggagaccct 26280
acactacacc ttccgccagg gctacgtgcg ccaggcttgc aagatctcca acgtggagct 26340
cagcaacctg gtgtcctacc tgggcatctt gcatgaaaac cgccttgggc agagcgtgct 26400
acactccacc ctgcgcgggg aggcgcgccg cgactacgtg cgcgactgcg tttacctctt 26460
cctctgctac acctggcaga cggccatggg ggtctggcag cagtgcctgg aggagcgcaa 26520
cctcaaggag ctggagaagc tcctgcagcg cgcgctcaaa gacctctgga cgggctacaa 26580
cgagcgctcg gtggccgccg cgctggccga cctcatcttc cccgagcgcc tgctcaaaac 26640
tctccagcag gggctgcccg acttcaccag ccaaagcatg ttgcaaaatt ttaggaactt 26700
tatcctggag cgttctggca tcctacccgc cacctgctgc gccctgccca gcgactttgt 26760
ccccctcgtg taccgcgagt gccccccgcc gctgtggggc cactgctacc tgttccaact 26820
ggccaactac ctgtcctacc acgcggacct catggaagac tccagcggcg aggggctcat 26880
ggagtgccac tgccgctgca acctctgcac gccccaccgc tccctggtct gcaacaccca 26940
actgctcagc gagagtcaga ttatcggtac cttcgagcta cagggtccgt cctcctcaga 27000
cgagaagtcc gcggctccgg ggctaaaact cactccgggg ctgtggactt ccgcctacct 27060
gcgcaaattt gtacctgaag actaccacgc ccacgagatc aggttttacg aggaccaatc 27120
ccgcccgccc aaggcggagc tgaccgcctg cgtcatcacc cagggcgaga tcctaggcca 27180
attgcaagcc atccaaaaag cccgccaaga gtttttgctg agaaagggtc ggggggtgta 27240
tctggacccc cagtcgggtg aggagctcaa cccggttccc ccgctgccgc cgccgcggga 27300
ccttgcttcc caggataagc atcgccatgg ctcccagaaa gaagcagcag cggccgccac 27360
tgccgccacc ccacacgctg gaggaagagg aggaatactg ggacagtcag gcagaggagg 27420
tttcggacga ggaggagccg gagacggaga tggaagagtg ggaggaggac agcttagacg 27480
aggaggcttc cgaagccgaa gaggcaggcg caacaccgtc accctcggcc gcagccccct 27540
cgcaggcgcc cccgaagtcc gctcccagca tcagcagcaa cagcagcgct ataacctccg 27600
ctcctccacc gccgcgaccc acggccgacc gcagacccaa ccgtagatgg gacaccaccg 27660
gaaccggggc cggtaagtcc tccgggaaag gcaagcaagc gcagcgccaa ggctaccgct 27720
cgtggcgcgc tcacaagaac gccatagtcg cttgcttgca agactgcggg gggaacatct 27780
ccttcgcccg ccgcttcctg ctcttccacc acggtgtggc cttcccccgt aacgtcctgc 27840
attactaccg tcatctctac agcccctact gcggcggcag tgagccagag gcggccggcg 27900
gcagcggcgc ccgtttcggt gcctaggaag acccagggca agacttcagc caagaaactc 27960
gcggcggccg cggcgaacgc ggtcgcgggg gccctgcgcc tgacggtgaa cgaacccctg 28020
tcgacccgcg aactgaggaa ccgaatcttc cccactctct atgccatctt ccagcagagc 28080
agagggcagg atcaggaact gaaagtaaaa aacaggtctc tgcgctccct cacccgcagc 28140
tgtctgtatc acaagagcga agaccagctt cggcgcacgc tggaggacgc tgaggcactc 28200
ttcagcaaat actgcgcgct cactcttaag gactagctcc gcgcccttct cgaatttagg 28260
cgggaacgcc tacgtcatcg cagcgccgcc gtcatgagca aggacattcc cacgccatac 28320
atgtggagct atcagccgca gatgggactc gcggcgggcg cctcccagga ttactccacc 28380
cgcatgaact ggctcagtgc cggcccacac atgatctcac aggttaatga catccgcacc 28440
catcgaaacc aaatattggt ggagcaggcg gcaattacca ccacgccccg caataatccc 28500
aaccccaggg agtggcccgc gtccctggtg tatcaggaaa ttcccggccc caccaccgta 28560
ctacttccgc gtgattccca ggccgaagtc caaatgacta actcaggggc acagctcgcg 28620
ggcggctgtc gtcacagggt gcggcctcct cgccagggta taactcacct gaagatccga 28680
ggcagaggta ttcagctcaa cgacgagtcg gtgagctcct cgctcggtct cagacctgac 28740
gggaccttcc agatagccgg agccggccga tcttccttca cgccccgcca ggcgtacctg 28800
actctgcaga gctcgtcctc ggcgccgcgc tcgggcggca tcgggactct ccagttcgtg 28860
caggagtttg tgccctcggt ctacttcaac cccttctcgg gctctcccgg tcgctacccg 28920
gaccagttca tcccgaactt tgacgccgcg agggactcgg tggacggcta cgactgaatg 28980
tcgggtggac ccggtgcaga gcaacttcgc ctgaagcacc ttgaccactg ccgccgccct 29040
cagtgctttg cccgctgtca gaccggtgag ttccagtact tttccctgcc cgactcgcac 29100
ccggacggcc cggcgcacgg ggtgcgcttt ttcatcccga gtcaggtccg ctctacccta 29160
atcagggagt tcaccgcccg tcccctactg gcggagttgg aaaaggggcc ttctatccta 29220
accattgcct gcatctgctc taaccctgga ttacaccaag atctttgctg tcatttgtgt 29280
gctgagtata ataaaggctg agatcagaat ctactcgggc tcctgtcgcc atcctgtcaa 29340
cgccaccgtc caagcccggc ccgatcagcc cgaggtgaac ctcacctgcg gtctgcaccg 29400
gcgcctgagg aaatacctag cttggtacta caacagcact ccctttgtgg tttacaacag 29460
ctttgaccag gacggggtct cactgaggga taacctctcg aacctgagct actccatcag 29520
gaagaacaac accctcgagc tacttcctcc ttacctgccc gggacttacc agtgtgtcac 29580
cggtccctgc acccacaccc acctgttgat cgtaaacgac tctcttccga gaacagacct 29640
caataactcc tctccgcagt tccccagaac aggaggtgag ctcaggaaac cccgggtaaa 29700
gaagggtgga caagagttaa cacttgtggg gtttctggtg tatgtgacgc tggtggtggc 29760
tcttttgatt aaggcttttc cttccatgtc tgaactatcc ctcttctttt atgaacaact 29820
cgactagtgc taacgggacc ctacccaacg aatcgggatt gaatatcggt aaccaggttg 29880
cagtttcact tttgattacc ttcatagtcc tcttcctgct agtgctgtcg cttctgtgcc 29940
tgcggatcgg gggctgctgc atccacgttt atatctggtg ctggctgttt agaaggttcg 30000
gagaccaccg caggtagaat aaacaaacct agacctagaa atggacggtc tctgcagcga 30060
gcaacgcata ctagagaggc gccggcaaaa agcagagctc gagcgtctta aacaagagct 30120
ccaagacgcc gtggccatac accagtgcaa aaaagggctc ttctgtctgg taaaacaggc 30180
cacgctcacc tatgaaaaaa caggtgacac ccaccgccta ggatacaagc tgcccacaca 30240
gcgccaaaag tttgccctta tgataggtga acaacccatc accgtgaccc agcactccgt 30300
ggagacagaa ggctgcattc atgctccctg caggggcgct gactgcctct acaccttgat 30360
caaaaccctt tgcggtctca gagaccttat ccctttcaat tgatcataac tgtaatcaat 30420
aaaaaatcac ttacttgaaa tctgatagca agcctctgtc caattttttc agcaacactt 30480
ccttcccctc ttcccaactc tggtactcta ggcgcctcct agctgcaaac ttcctccaca 30540
gtctgaaggg aatgtcagat tcctcctcct cctgtccctc cgcacccacg atcttcatgt 30600
tgttgcagat gaagcgcacc aaaacgtctg acgagagctt caaccccgtg tacccctatg 30660
acacggaaaa cggtcctccc tccgtccctt tcctcacccc tcccttcgtg tctcccgatg 30720
gattccaaga gagccccccc ggggtcctgt ctctgaacct ggccgagccc ctggtcactt 30780
cccacggcat gctcgccctg aaaatgggaa gtggcctctc cctggacgac gccggcaacc 30840
tcacctctca agatgtcacc accactaccc ctcccctgaa aaaaaccaag accaacctca 30900
gcctagaaac ctcagccccc ctgactgtga gcacctcagg cgccctcacc ctagcagccg 30960
ccgttcccct ggcggtggcc ggcacctccc tcaccatgca atcagaggcc cccctgacag 31020
tccaagatgc aaaactcacc ctggccacca agggccccct gaccgtgtct gaaggcaaac 31080
tggccttgca gacctcggcc ccgctgacgg ccgctgacag cagcgccctc accgttagcg 31140
ccacaccacc catcagtgta agcagtggaa gtttgggctt agacatggaa gaccccatgt 31200
atactcatga tggaaaactg ggaataagaa ttggaggccc actgagagta gtagacagcc 31260
tgcacacact gactgtagtt accggaaatg gaatagctgt agataacaat gccctccaaa 31320
ctagagttac gggcgccctg ggttatgaca catcaggaaa cctacaactg agagccgcgg 31380
ggggtatgcg aattgatgca aatggccaac ttatccttga tgtggcatac ccatttgatg 31440
ctcaaaacaa tctcagcctt agacttggtc agggacccct gtatgtaaac acagaccaca 31500
acctagattt gaattgcaac agaggtctga ccacaactac caccaacaac acaaaaaaac 31560
ttgaaactaa aattggctca ggcttagact atgataccaa tggtgctgtc attattaaac 31620
ttggtactgg tgtaagcttt gacagcacag gcgccctaac tgtgggaaac actggcgatg 31680
ataaactgac tctgtggaca accccagacc catctccaaa ttgcagaatt cacgcagaca 31740
aagactgcaa gtttactcta gtcctaacta agtgtggaag tcaaatcctg gcttctgtcg 31800
ccgccctagc ggtgtcagga aatctggctt caataacagg caccgttgcc agcgttacca 31860
tctttctcag atttgatcag aatggagtgc ttatggaaaa ctcctcccta gacaagcagt 31920
actggaactt cagaaatggt aactcaacca atgccacccc ctacaccaat gcagttgggt 31980
tcatgccaaa cctcgcagca taccccaaga cacagagcca gactgctaaa aacaacattg 32040
taagtcaggt ttacttgaat ggggacaaat ccaaacccat gacccttacc attaccctca 32100
atggaactaa tgaatccagt gaaactagcc aggtgagtca ctactccatg tcatttacat 32160
gggcttggga gagtgggcaa tatgccaccg aaacctttgc caccaattcc tttaccttct 32220
cttacattgc tgaacaataa agaaagcaca gagatgcttg tttttgattt caaaattgtg 32280
tgcttttatt tattttcaag cttacagtat ttccagtagt cattcgaata gagcttaatg 32340
aaactgcatg agaacccttc cacatagctt aaattatcac cagtgcaaat ggagaaaaat 32400
caacatacct ttttatccag atatcacaga accctagtat tcaacctgcc acctccctcc 32460
caacacacag agtacacagt cctttctccc cggctggcct taaaaagcat catatcatgg 32520
gtaacagaca tattcttagg tgttatattc cacacggttt cctgtcgagc caaacgctca 32580
tcagtgatat taataaactc cccgggcagc tcacttaagt tcatgtcgct gtccagctgc 32640
tgagccacag gctgctgtcc aacttgcggt tgcttaacgg gcggcgaagg agaagtccac 32700
gcctacatgg gggtagagtc ataatcgtgc atcaggatag ggcggtggtg ctgcagcagc 32760
gcgcgaataa actgctgccg ccgccgctcc gtcctgcagg aatacaacat ggcagtggtc 32820
tcctcagcga tgattcgcac cgcccgcagc ataaggcgcc ttgtcctccg ggcacagcag 32880
cgcaccctga tctcacttaa atcagcacag taactgcagc acagcaccac aatattgttc 32940
aaaatcccac agtgcaaggc gctgtatcca aagctcatgg cggggaccac agaacccacg 33000
tggccatcat accacaagcg caggtagatt aagtggcgac ccctcataaa cacgctggac 33060
ataaacatta cctcttttgg catgttgtaa ttcaccacct cccggtacca tataaacctc 33120
tgattaaaca tggcgccatc caccaccatc ctaaaccagc tggccaaaac ctgcccgccg 33180
gctatacact gcagggaacc gggactggaa caatgacagt ggagagccca ggactcgtaa 33240
ccatggatca tcatgctcgt catgatatca atgttggcac aacacaggca cacgtgcata 33300
cacttcctca ggattacaag ctcctcccgc gttagaacca tatcccaggg aacaacccat 33360
tcctgaatca gcgtaaatcc cacactgcag ggaagacctc gcacgtaact cacgttgtgc 33420
attgtcaaag tgttacattc gggcagcagc ggatgatcct ccagtatggt agcgcgggtt 33480
tctgtctcaa aaggaggtag acgatcccta ctgtacggag tgcgccgaga caaccgagat 33540
cgtgttggtc gtagtgtcat gccaaatgga acgccggacg tagtcatatt tcctccagca 33600
gaaccaagtg cgcgcgtggc agctatcctt gcgtcttctg tctcgccgcc tgccccgctc 33660
ggtgtagtag ttgtaataca gccactccct cagaccgtca aggcgctccc tggcgtccgg 33720
atctataaca acaccatcct gcagcgccgc cctgatgaca tccaccaccg tagagtatgc 33780
caagcccagc caggaaatgc actcactttg acagcgagag ataggaggag cgggaagaga 33840
tggaagaacc atgatagtaa aagaactttt attccaatcg atcctctaca atgtcaaagt 33900
gtagatctat cagatggcac tggtctcctc cgctgagtcg atcaaaaata acagctaaac 33960
cacaaacaac acgattggtc aaatgctgca caagggcttg cagcataaaa tcgcctcgaa 34020
agtccaccgc aagcataaca tcaaagccac cgcccctatc atgatctatg ataaaaaccc 34080
cacagctatc caccagaccc atatagtttt catctctcca tcgtgaaaaa atatttacaa 34140
gctcctcctt taaatcacct ccaaccaatt caaaaagttg agccagaccg ccctccacct 34200
tcattttcag catgcgcatc atgattgcaa aaattcaggc tcctcagaca cctgtataag 34260
attgagaagc ggaacattaa catcaatgtt tcgctcgcga agatcgcgcc tcagtgcaag 34320
catgatataa tcccacaggt cggagcggat cagcgaggac atctccccgc caggaaccaa 34380
ctcaacggag cctatgctga ttataatacg catattcggg gctatgctaa ccagcacggc 34440
ccccaaatag gcgtactgca taggcggcga caaaaagtga acagtttggg ttaaaaaatc 34500
aggcaaacac tcgcgcaaaa aagcaagaac atcataacca tgctcatgca aatagatgca 34560
agtaagctca ggaacgacca cagaaaaatg cacaattttt ctctcaaaca tgactgcgag 34620
ccctgcaaaa aataaaaaag aaacattaca caagagtagc ctgtcttaca atgggataga 34680
ctactctaac caacataaga cgggccacga catcgcccgc gtggccataa aaaaaattat 34740
ccgtgtgatt aaaaagaagc acagatagct ggccagtcat atccggagtc atcacgtgcg 34800
aacccgtgta gacccccggg ttggacacat cggccaaaga aagaaagcgg ccaatgtatc 34860
ccggaggaat gataacacta agacgaagat acaacagaat aaccccatgg gggggaataa 34920
caaagttagt aggtgaataa aaacgataaa cacccgaaac tccctcctgc gtaggcaaaa 34980
tagcgccctc cccttccaaa acaacataca gcgcttccac agcagccatg acaaaagact 35040
caaaacactc aaaagactca gtcttaccag gaaaataaaa gcactctcac agcaccagca 35100
ctaatcagag tgtgaagagg gccaagtgcc gaacgagtat atataggaat taaaaatgac 35160
gtaaatgtgt aaaggtcaaa aaacgcccag aaaaatacac agaccaacgc ccgaaacgaa 35220
aacccgcgaa aaaataccca gaagttcctc aacaaccgcc acttccgctt tcccacgata 35280
cgtcacttcc tcgaaaatag caaactacat ttcccacatg tacaaaaccg aaaccactcc 35340
ccttgtcacc gcccacaact tacatcttaa ttaacaaacg tcaaagccta cgtcagccgc 35400
cccgcctcgc cccgcccacc tcattatcat attggccaca atccaaaata aggtatatta 35460
ttgatgatga tttaaatatt atgcggtgtg aaataccgca cagatgcgta aggagaaaat 35520
accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 35580
tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 35640
ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 35700
ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 35760
gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 35820
gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 35880
ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 35940
tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 36000
gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 36060
tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 36120
tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 36180
tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 36240
ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 36300
ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 36360
gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 36420
aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 36480
aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 36540
cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 36600
ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 36660
cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 36720
ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 36780
ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 36840
ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 36900
gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 36960
ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 37020
ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 37080
gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 37140
ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 37200
cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 37260
ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 37320
aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 37380
gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 37440
gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa 37500
cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattgatt taaat 37555
<210> 17
<211> 34477
<212> DNA
<213> 人工序列
<220>
<223> BZ1/BZ28F.Fluc腺病毒载体
<400> 17
catcatcaat aatatacctt attttggatt gtggccaata tgataatgag gtgggcgggg 60
cgaggcgggg cgggtgacgt aggacgtgcg agtagggttg ggaggtgtgg cggaagtgtg 120
gcatttgtaa gtgggaggag cttacatgca agcttccgtc gcggaaaatg tgacgttttt 180
tatgagcgcc gcctacctcc ggaagtgcca attttcgcgc gctttttacc ggatatcgta 240
gtaattttgg gcgggaccat gtaagatttg gccattttcg cgcgaaaagt gaaacgggga 300
agtgaaaact gaataatagg gcgttagtca tagcgcgtaa tatttaccga gggccgaggg 360
actttgaccg attacgtgga ggactcgccc aggtgttttt tacgtgaatt tccgcgttcc 420
gggtcaaagt ctccgttttt attgtcaccg tcatttgacg cggagggtat ttaaacccgc 480
tgcgctccta gcgatcgctc aatattggcc attagccata ttattcattg gttatatagc 540
ataaatcaat attggctatt ggccattgca tacgttgtat ccatatcata atatgtacat 600
ttatattggc tcatgtccaa cattaccgcc atgttgacat tgattattga ctagttatta 660
atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata 720
acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat 780
aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga 840
gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc 900
ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt 960
atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat 1020
gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag 1080
tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc 1140
aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga 1200
ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg 1260
ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggcc gggaacggtg 1320
cattggaagc ttggcattcc ggtactgttg gtaaagccac catggaagac gccaaaaaca 1380
taaagaaagg cccggcgcca ttctatccgc tggaagatgg aaccgctgga gagcaactgc 1440
ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata 1500
tcgaggtgga catcacttac gctgagtact tcgaaatgtc cgttcggttg gcagaagcta 1560
tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc 1620
aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg 1680
acatttataa tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct accgtggtgt 1740
tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc ccaatcatcc 1800
aaaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg atgtacacgt 1860
tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtgcca gagtccttcg 1920
atagggacaa gacaattgca ctgatcatga actcctctgg atctactggt ctgcctaaag 1980
gtgtcgctct gcctcataga actgcctgcg tgagattctc gcatgccaga gatcctattt 2040
ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc catcacggtt 2100
ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc ttaatgtata 2160
gatttgaaga agagctgttt ctgaggagcc ttcaggatta caagattcaa agtgcgctgc 2220
tggtgccaac cctattctcc ttcttcgcca aaagcactct gattgacaaa tacgatttat 2280
ctaatttaca cgaaattgct tctggtggcg ctcccctctc taaggaagtc ggggaagcgg 2340
ttgccaagag gttccatctg ccaggtatca ggcaaggata tgggctcact gagactacat 2400
cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt aaagttgttc 2460
cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc gttaatcaaa 2520
gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac aatccggaag 2580
cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata gcttactggg 2640
acgaagacga acacttcttc atcgttgacc gcctgaagtc tctgattaag tacaaaggct 2700
atcaggtggc tcccgctgaa ttggaatcca tcttgctcca acaccccaac atcttcgacg 2760
caggtgtcgc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc gttgttgttt 2820
tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa 2880
caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta 2940
ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag aagggcggaa 3000
agatcgccgt gtaattctag acgagatccg aacttgttta ttgcagctta taatggttac 3060
aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 3120
tgtggtttgt ccaaactcat caatgtatct tatcatgtct gcgatcgctg atgagaccag 3180
gaccaggtgc cgaccctgcg agtgcggcgg caagcacatg agaaatcagc ctgtgatgtt 3240
ggatgtgacc gaggagctta ggcctgacca tctggtgctg gcctgcacca gggccgagtt 3300
tgggtctagc gatgaggata ccgattgagg tgggtaaggt gggcgtggct agcagggtgg 3360
gcgtgtataa attgggggtc taaggggtct ctctgtttgt cttgcaacag ccgccgccat 3420
gagcgacacc ggcaacagct ttgatggaag catctttagc ccctatctga cagtgcgcat 3480
gcctcactgg gccggagtgc gtcagaatgt gatgggttcc aacgtggatg gacgtcccgt 3540
tctgccttca aattcgtcta cgatggccta cgcgaccgtg ggaggaactc cgctggacgc 3600
cgcgacctcc gccgccgcct ccgccgccgc cgcgaccgcg cgcagcatgg ctacggacct 3660
ttacagctct ttggtggcga gcagcgcggc ctctcgcgcg tctgctcggg atgaaaaact 3720
gactgctctg ctgcttaaac tggaagactt gacccgggag ctgggtcaac tgacccagca 3780
ggtctccagc ttgcgtgaga gcagccttgc ctccccctaa tggcccataa tataaataaa 3840
agccagtctg tttggattaa gcaagtgtat gttctttatt taactctccg cgcgcggtaa 3900
gcccgggacc agcggtctcg gtcgtttagg gtgcggtgga ttctttccaa cacgtggtac 3960
aggtggctct ggatgtttag atacatgggc atgagtccat ccctggggtg gaggtagcac 4020
cactgcagag cttcgtgctc gggggtggtg ttgtatatga tccagtcgta gcaggagcgc 4080
tgggcgtggt gctgaaaaat gtccttaagc aagaggctta tagctagggg gaggcccttg 4140
gtgtaagtgt ttacaaatct gcttagctgg gaggggtgca tccgggggga tatgatgtgc 4200
atcttggact ggatttttag gttggctatg ttcccaccca gatcccttct gggattcatg 4260
ttgtgcagga ccaccagcac ggtatatcca gtgcacttgg gaaatttatc gtggagctta 4320
gacgggaatg catggaagaa cttggagacg cccttgtggc ctcccagatt ttccatacat 4380
tcgtccatga tgatggcaat gggcccgtgg gaagctgcct gagcaaaaac gtttctggca 4440
tcgctcacat cgtagttatg ttccagggtg aggtcatcat aggacatctt tacaaatcgg 4500
gggcggaggg tcccggactg ggggatgatg gtaccctcgg gccccggggc gtagttcccc 4560
tcacagatct gcatctccca ggctttcatt tcagagggag ggatcatatc cacctgcggg 4620
gcgatgaaaa agacagtttc tggcgcaggg gagattaact gggatgagag caggtttctg 4680
agcagctgtg actttccaca gccggtgggc ccatatatca cgcctatcac cggctgcagc 4740
tggtagttaa gagagctgca gctgccgtcc tcccggagca ggggggccac ctcgttgagc 4800
atatccctga cgtggatgtt ctccctgacc agttccgcca gaaggcgctc gccgcccagc 4860
gaaagcagct cttgcaagga agcaaaattt ttcagcggtt tcaggccatc ggccgtgggc 4920
atgtttttca gcgtctgggt cagcagctcc agcctgtccc agagctcggt gatgtgctct 4980
acggcatctc gatccagcag atctcctcgt ttcgcgggtt ggggcggctt tcgctgtagg 5040
gcaccagccg atgggcgtcc agcggggcca gagtcatgtc cttccatggg cgcagagtcc 5100
tcgtcagggt ggtctgggtc acggtgaagg ggtgcgctcc gggttgggca ctggccaggg 5160
tgcgcttgag gctggttctg ctggtgctga atcgctgccg ctcttcgccc tgcgcgtcgg 5220
ccaggtagca tttgaccatg gtctcgtagt cgagaccctc ggcggcgtgc cccttggcgc 5280
ggagctttcc cttggaggtg gcgccgcacg aggggcactg caggctcttc agggcgtaga 5340
gcttgggagc gagaaacacg gactctgggg agtaggcgtc cgcgccgcag gccgcgcaga 5400
ccgtctcgca ttccaccagc caagtgagtt ccgggcggtc agggtcaaaa accaggttgc 5460
ccccatgctt tttgatgcgt ttcttacctc ggctctccat gaggcggtgt cccttctcgg 5520
tgacgaagag gctgtccgtg tccccgtaga ccgacttcag gggcctgtct tccagcggag 5580
tgcctctgtc ctcctcgtag agaaactctg accactctga gacgaaggcc cgtgtccagg 5640
ccaggacgaa ggaggccacg tgggaggggt agcggtcgtt gtccactagc gggtccacct 5700
tctccagggt gtgcagacac atgtccccct cctccgcgtc cagaaaagtg attggcttat 5760
aggtgtagga cacgtgaccg ggggttcccg acgggggggt ataaaagggg gtgggcgccc 5820
tttcatcttc actctcttcc gcatcgctgt ctgcgagagc cagctgctgg ggtaagtatt 5880
ccctttcaaa ggcgggcatg acctcagcgc tcaggttgtc agtttctaaa aatgaggagg 5940
atttgatgtt cacctgtccg gaagtgatac ctttgagggt acctgggtcc atctggtcag 6000
aaaacactat ttttttgttg tcaagcttgg tggcgaacga cccgtagagg gcgttggaga 6060
gcagcttggc aatggagcgc agggtctggt ttttgtcgcg gtcggctcgc tccttggccg 6120
cgatgttgag ttgcacgtat tcgcgggcca cgcacttcca ctcggggaag acggtggtgc 6180
gctcgtctgg gatcaggcgc accctccagc cgcggttgtg cagggtgacc atgtcgacgc 6240
tggtggcgac ctcaccgcgc agacgctcgt tggtccagca gaggcggccg cctttgcgcg 6300
agcagaaggg gggtaggggg tccagctggt cctcgtttgg ggggtccgcg tcgatggtaa 6360
agaccccggg gagcaggcgc gggtcaaagt agtcgatctt gcaagcttgc atgtccagag 6420
cccgctgcca ttcgcgggcg gcgagcgcgc gctcgtaggg gttgaggggc gggccccagg 6480
gcatggggtg ggtgagcgcg gaggcgtaca tgccgcagat gtcatacacg tacaggggtt 6540
ccctgaggat accgaggtag gtggggtagc agcgcccccc gcggatgctg gcgcgcacgt 6600
agtcatagag ctcgtgggag ggggccagca tgttgagccc aaggttggtg cgctgggggc 6660
gctcggcgcg gaagacgatc tgtctgaaga tggcatggga gttggaggag atggtgggtc 6720
gctggaagac gttgaagctt gcttcttgca agcccacgga gtccctgacg aaggaggcgt 6780
aggactcgcg cagcttgtgc accagctcgg cggtgacctg gacgtcgagc gcgcagtagt 6840
cgagggtctc gcggatgatg tcatacttat cctccccctt ctttttccac agctcgcggt 6900
tgaggacgaa ctcttcgcgg tctttccagt actcttggag gggaaacccg tccgtgtccg 6960
aacggtaaga gcctagcatg tagaactggt tgacggcctg gtaggggcag cagcccttct 7020
ccacgggcag cgcgtaggcc tgcgccgcct tgcggaggga ggtgtgggta agggcgaaag 7080
tgtccctgac catgactttg aggtattgat gtctgaagtc tgtgtcatcg cagccgccct 7140
gttcccacag ggtgtagtcc gtgcgctttt tggagcgcgg gttgggcagg gagaaggtga 7200
ggtcattgaa gaggatcttc cccgctcgag gcatgaagtt tctggtgatg cgaaagggcc 7260
ctgggaccga ggagcggttg ttgatgacct gggcggccag gacgatctcg tcaaagccgt 7320
ttatgttgtg gcccacgatg tagagctcca ggaagcgggg ctggcccttg atggagggga 7380
gctttttaag ttcctcgtag gtaagctcct cgggcgattc caggccgtgc tcctccaggg 7440
cccagtcttg caagtgaggg ttggccgcca ggaaggatcg ccagaggtcg cgggccatga 7500
gggtctgcag gcggtcgcgg aaggttctga actgccgccc tacggccatc ttttcggggg 7560
tgatgcagta gaaggtgagg gggtctttct cccaggggtc ccatctgagc tcttgggcga 7620
ggtcgcgcgc ggcggcgacc agagcctcgt cgccccccag tttcatgacc agcatgaagg 7680
gcacgagctg cttgccaaag gctcccatcc aagtgtaggt ctctacatcg taggtgacaa 7740
agaggcgctc cgtgcgagga tgagagccga tcgggaagaa ctggatctcc cgccaccagt 7800
tggaggattg gctgttgatg tggtgaaagt agaagtcccg tctgcgggcc gagcactcgt 7860
gctggctttt gtaaaagcga ccgcagtact ggcagcgctg cacgggttgt atatcttgca 7920
cgaggtgaac ctggcgacct ctgacgagga agcgcagcgg gaatctaagt cccccgcctg 7980
gggtcccgtg tggctggtgg tcttctactt tggttgtctg gccgccagca tctgtctcct 8040
ggagggcgat ggtggagcag accaccacgc cgcgagagcc gcaggtccag atctcggcgc 8100
tcggcgggcg gagtttgatg acgacatcgc gcacattgga gctgtccatg gtctccagct 8160
cccgcggcgg caggtcagcc gggagttcct ggaggttcac ctcgcagaga cgggtcaagg 8220
cgcggacagt gttgagatgg tatctgattt caaggggcgt gttggaggcg gagtcgatgg 8280
cttgcagaag gccgcagccc cggggggcca cgatggttcc ccgcggggcg cgaggggagg 8340
cggaagctgg gggtgtgttc agaagcggtg acgcgggcgg gcccccggag gtaggggggg 8400
ttccggcccc acaggcatgg gcggcagggg cacgtcttcg ccgcgcgcgg gcaggggctg 8460
gtgctggctc cggagagcgc ttgcgtgcgc gacgacgcga cggttggtgt cctgtatctg 8520
gcgcctctga gtgaagacca cgggtcccgt gaccttgaac ctgaaagaga gttcgacaga 8580
atcaatctcg gcatcgttga cagcggcctg gcgcaggatc tcctgcacgt cgcccgagtt 8640
gtcctggtag gcgatctctg ccatgaactg ctcgatctct tcctcctgga gatctcctcg 8700
tccggcgcgc tccacggtgg ccgccaggtc gttggagatg cgacccatga gctgcgagaa 8760
ggcgttgagg ccgccctcgt tccagacccg gctgtagacc acgcccccct cggcgtcgcg 8820
ggcgcgcatg accacctggg ccaggttgag ctccacgtgt cgcgtgaaga cggcgtagtt 8880
gcgcaggcgc tggaaaaggt agttcagggt ggtggcggtg tgttcggcga cgaagaagta 8940
catgacccag cgccgcaacg tggattcatt gatgtccccc aaggcctcca ggcgctccat 9000
ggcctcgtag aagtccacgg cgaagttgaa aaactgggag ttgcgagcgg acacggtcaa 9060
ctcctcctcc agaagacgga tgagctcggc gacagtgtcg cgcacctcgc gctcgaaggc 9120
cacggggggc gcttcttcct cttccacctc ttcttccatg attgcttctt cttcctcagc 9180
cgggacggga gggggcggcg gcgggggagg ggcgcggcgg cggcggcggc gcaccggcag 9240
gcggtcgatg aagcgctcga tcatctcccc ccgcatgcgg cgcatggtct cggtgacggc 9300
gcggccgttc tcccgggggc gcagctcaaa gacgccgcct ctcatctcgc cgcggggcgg 9360
gcggccgtga ggtagcgaga cggcgctgac tatgcatctt aacaattgct gtgtaggtac 9420
gccgccaagg gacctgattg agtccagatc caccggatcc gaaaaccttt ggaggaaagc 9480
gtctatccag tcgcagtcgc aaggtaggct gagcaccgtg gcgggcgggg gcgggtcggg 9540
agagttcctg gcggagatgc tgctgatgat gtaattaaag taggcggtct tgagaaggcg 9600
gatggtggac aggagcacca tgtctttggg tccggcctgt tggatgcgga ggcggtcggc 9660
catgccccag gcctcgttct gacaccggcg caggtctttg tagtagtctt gcatgagtct 9720
ttccaccggc acctcttctc cttcctcttc tccatctcgc cggtggtttc tcgcgccgcc 9780
catgcgcgtg accccaaagc ccctgagcgg ctgcagcagg gccaggtcgg cgaccacgcg 9840
ctcggccaag atggcctgct gtacctgagt gagggtcctc tcgaagtcat ccatgtccac 9900
gaagcggtgg taggcgcccg tgttgatggt gtaggtgcag ttggccatga cggaccagtt 9960
gacggtctgg tgtcccggct gcgagagctc cgtgtaccgc aggcgcgaga aggcgcggga 10020
atcgaacacg tagtcgttgc aagtccgcac cagatactgg tagcccacca ggaagtgcgg 10080
cggaggttgg cgatagaggg gccagcgctg ggtggcgggg gcgccgggcg ccaggtcttc 10140
cagcatgagg cggtggtatc cgtagatgta cctggacatc caggtgatgc cggcggcggt 10200
ggtggtggcg cgcgcgtagt cgcggacccg gttccagatg tttcgcaggg gcgagaagtg 10260
ttccatggtc ggcacgctct ggccggtgag gcgcgcgcag tcgttgacgc tctatacaca 10320
cacaaaaacg aaagcgttta cagggctttc gttctgtagc ctggaggaaa gtaaatgggt 10380
tgggttgcgg tgtgccccgg ttcgagacca agctgagctc ggccggctga agccgcagct 10440
aacgtggtat tggcagtccc gtctcgaccc aggccctgta tcctccagga tacggtcgag 10500
agcccttttg ctttcttggc caagcgcccg tggcgcgatc tgggatagat ggtcgcgatg 10560
agaggacaaa agcggctcgc ttccgtagtc tggagaaaca atcgccaggg ttgcgttgcg 10620
gcgtaccccg gttcgagccc ctatggcggc ttggatcggc cggaaccgcg gctaacgtgg 10680
gctgtggcag ccccgtcctc aggaccccgc cagccgactt ctccagttac gggagcgagc 10740
cccttttgtt ttttattttt tagatgcatc ccgtgctgcg gcagatgcgc ccctcgcccc 10800
ggcccgatca gcagcagcaa cagcaggcat gcagaccccc ctctcctcta cccgccccgg 10860
tcaccacggc cgcggcggcc gtgtccggcg cggggggcgc gctggagtca gatgagccac 10920
cgcggcggcg acctaggcag tatctggact tggaagaggg cgagggactg gcgcggctgg 10980
gggcgagctc tccagagcgc cacccgcggg tgcagttgaa aagggacgcg cgcgaggcgt 11040
acctgccgcg gcagaacctg tttcgcgacc gcgggggcga ggagcccgag gagatgcgag 11100
actgcaggtt ccaagcgggg cgcgagctgc gccgcgggtt ggacagacag cgcctgctgc 11160
gcgaggagga ctttgagccc gacacgcaga cgggcatcag ccccgcgcgc gcgcacgtgg 11220
ccgcggccga cctggtgacc gcctacgagc agacggtgaa ccaggagcgc aacttccaaa 11280
aaagcttcaa caaccacgtg cgcacgctgg tggcgcgcga ggaggtgacc ctgggtctca 11340
tgcatctgtg ggacctggtg gaggcgatcg tgcagaaccc cagcagcaag cccctgaccg 11400
cgcagctgtt cctggtggtg cagcacagca gggacaacga ggccttcagg gaggcgctgc 11460
tgaacatcac cgagccggag gggcgctggc tcctggacct gataaacatc ctgcagagca 11520
tagtggtgca ggagcgcagc ctgagcctgg ccgagaaggt ggcggccatt aactattcta 11580
tgctgagctt gggcaagttc tacgcccgca agatctacaa gaccccctac gtgcccatag 11640
acaaggaggt gaagatagac agcttctaca tgcgcatggc gctaaaggtg ctgaccctga 11700
gcgacgacct gggagtgtac cgcaacgagc gcatccacaa ggccgtgagc gccagccggc 11760
ggcgcgagct gagcgaccgc gagctgatgc acagtctgca gcgcgcgctc accggcgcgg 11820
gcgagggcga cagggaggtc gagtcctact tcgacatggg ggctgacctg cactggcagc 11880
cgagccgccg cgccctggag gcggcggggg cgtatggcgg ccccctggcg gccgatgacg 11940
aggaagagga ggactatgag ctagaggagg gcgagtacct ggaggactga cctggctggt 12000
ggtgttttgg tatagatgca agatccgaac gtggcggacc cggcggtccg ggcggcgctg 12060
cagagccagc cgtccggcat taactcctct gacgactggg ccgcggccat gggtcgcatc 12120
atggccctga ccgcgcgcaa ccccgaggcc ttcaggcagc agcctcaggc taaccggctg 12180
gcggccatct tggaagcggt agtgcccgcg cgctccaacc ccacccacga gaaggtgctg 12240
gccatagtca acgcgctggc ggagagcagg gccatccggg cggacgaggc cggactggtg 12300
tacgatgcgc tgctgcagcg ggtggcgcgg tacaacagcg gcaacgtgca gaccaacctg 12360
gaccgcctgg tgacggacgt gcgcgaggcc gtggcgcagc gcgagcgctt gcatcaggac 12420
ggtaacctgg gctcgctggt ggcgctaaac gccttcctca gcacccagcc ggccaacgta 12480
ccgcgggggc aggaggacta caccaacttc ttgagcgcgc tgcggctgat ggtgaccgag 12540
gtccctcaga gcgaggtgta ccagtcgggg cccgactact tcttccagac cagcagacag 12600
ggcttgcaaa ccgtgaacct gagccaggct ttcaagaacc tgcgggggct gtggggagtg 12660
aaagcgccca ccggcgaccg agctacggtg tccagcctgc taacccccaa ctcgcgcctg 12720
ctgctgctgc tgatcgcgcc cttcacggac agcgggagcg tctcgcggga gacctatctg 12780
ggccacctgc tgacgctgta ccgcgaggcc atcgggcagg cgcaggtgga cgagcacacc 12840
ttccaagaga tcaccagcgt gagccacgcg ctggggcagg aggacacggg cagcctgcag 12900
gcgaccctga actacctgct gaccaacagg cggcagaaga ttcccacgct gcacagcctg 12960
acccaggagg aggagcgcat cttgcgctac gtgcagcaga gcgtgagcct gaacctgatg 13020
cgcgacggcg tgacgcccag cgtggcgctg gacatgaccg cgcgcaacat ggaaccgggc 13080
atgtacgctt cccagcggcc gttcatcaac cgcctgatgg actacttgca tcgggcggcg 13140
gccgtgaacc ccgagtactt caccaatgcc attctgaatc cccactggat gccccctccg 13200
ggtttctaca acggagactt cgaggtgcct gaggtcaatg atgggttcct ctgggatgac 13260
atggatgaca gtgtgttctc ccccaacccg ctgcgcgccg cgtctctgcg attgaaggag 13320
ggctctgaca gggaaggacc gaggagtctg gcctcctccc tggctctggg ggcggtgggc 13380
gccacgggcg cggcggcgcg gggcagcagc cccttcccca gcctggcaga ctctctgaat 13440
agcgggcggg tgagcaggcc ccgcttgcta ggcgaggagg agtatctgaa caactccctg 13500
ctgcagcccg tgagggacaa aaacgctcag cgacagcagt ttcccaacaa cgggatagag 13560
agcctggtgg acaagatgtc cagatggaag acatatgcgc aggagtacaa ggagtgggag 13620
gaccgccagc cgcggcccct gccgccccct agacagcgct ggcagcggcg cgcgtccaac 13680
cgccgctgga gacagggacc cgaggacgat gatgactctg cagatgacag cagcgtgttg 13740
gacctgggcg ggagcgggaa ccccttttcg cacctgcgcc cacgcctggg caagatgttt 13800
taaaagagaa aaataaaact caccaaggcc atggcgacga gcgttggttt tttgttccct 13860
tccttagtat gcggcgcgcg gcgatgttcg aggaggggcc tcccccctct tacgagagcg 13920
cgatgggaat ttctcctgcg gcgcccctgc agcctcccta cgtgcctcct cggtacctgc 13980
aacctacagg ggggagaaat agcatctgtt actctgagct gcagcccctg tacgatacca 14040
ccagactgta cctggtggac aacaagtccg cggacgtggc ctccctgaac taccagaacg 14100
accacagcga ttttttgacc acggtgatcc aaaacaacga cttcacccca accgaggcca 14160
gtacccagac cataaacctg gacaacaggt cgaactgggg cggcgacctg aagaccatcc 14220
tgcacaccaa catgcccaac gtgaacgagt tcatgtttac caactctttt aaggcgcggg 14280
ttatggtggc gcgcgagcag ggggaggcga agtacgagtg ggtggacttc acgctgcccg 14340
agggcaacta ctcagagacc atgactattg acctgatgaa caatgcgatc gtggaacact 14400
acctgaaagt gggcaggcag aacggggtga aggagagcga tatcggggtc aagtttgaca 14460
ccagaaactt tcgtctgggc tgggaccccg tgaccgggct ggtcatgccg ggggtctaca 14520
ccaacgaggc ctttcatccc gatatagtgc tcctgcccgg ctgtggggtg gactttaccc 14580
agagccggct gagcaacctg ctgggcgttc gcaagcggca acctttccag gagggtttca 14640
agatcaccta tgaggatctg gaggggggca acattcccgc gctccttgat ctggacgcct 14700
acgaggagag cttgaaaccc gaggagagcg ctggcgacag cggcgagagt ggcgaggagc 14760
aagccggcgg cggtggcagc gcgtcggtag aaaacgaaag tactcccgca gtggcggcag 14820
acgctgcgga ggtcgagcca gaggccatgc agcaggacgc agaggagggc gcacaggagg 14880
gcgcgcagga ggacatgaac gatggggaga tcaggggaga cactttcgcc acccggggcg 14940
aagaaaaaga ggcagaggcg gtggcggcga cggtggaagc cgaaaccgag gcagaggcag 15000
agcccaagac cgaagttatg gaagacatga atgatggaga acgtaggggt gacacgtttg 15060
ccacccgggg cgaagagaag gcggcggagg cagaagccgc ggctgaggag gcggctgcgg 15120
ctgcggccga ggctgaggct gcggctgagg ctaaggtcga agccgatgtt gcggttgagg 15180
ctcaggctga ggaggaggag gcggcgactg aagcagttaa ggaaaaggcc caggcagagc 15240
aggaagagaa aaaacctgtc attcaacctc taaaagaaga tagcaaaaag cgcagttaca 15300
acgtcatcga gggcagcacc tttacccagt accgcagctg gtacctggcg tacaactacg 15360
gcgacccggt caagggggtg cgctcgtgga ccctgctctg cacgccggac gtcacctgcg 15420
gctccgagca gatgtattgg tcgctgccaa acatgatgca agacccggtg accttccgct 15480
ccacgcggca ggttagcaac ttcccggtgg tgggcgccga actgctgccc gtgcactcca 15540
agagttttta caacgagcag gccgtctact cccagctgat ccgccaggcc acctctctga 15600
cccacgtgtt caatcgcttt cccgagaacc agattttggc gcgcccgccg gcccccacca 15660
tcaccaccgt cagtgaaaac gttcctgccc tcacagatca cgggacgcta ccgctgcgca 15720
acagcatctc aggagtccag cgagtgacca ttactgacgc cagacgccgg acctgcccct 15780
acgtttacaa ggccttgggc atagtctcgc cgcgcgtcct ctccagtcgc actttttaaa 15840
atacatctac cctcacgctt caaaatcatg tccgtactca tctcacccag caacaacacc 15900
ggctgggggc tgcgcgcgcc cagcaagatg tttggagggg cgaggaaacg ctccgagcag 15960
cacccagtgc gcgtgcgcgg ccactaccgc gcgccctggg gagcgcacaa gcgcgggcgc 16020
gcagggcgca ccactgtgga cgacgccatt gactccgtag tggagcaggc gcgccactac 16080
acacccggcg cgccgtccgc ccccgccgtg tccaccgtgg acgaggcgat cgagagcgtg 16140
gtacagggcg cgcggcacta tgccaacctt aaaaatcgac gccgtcgcgt ggctcgccgc 16200
catcgccgga gaccccgggc caccgccgcc gcgcgccttg ctaaggctct gctcaggcgc 16260
gccaggcgaa ctggccgccg ggccgccatg agggccgcac ggcgggctgc cgccagcgcg 16320
gccgccgcgg ccccacgggc acgaaggcgc gcggccgctg ccgccgccgc cgccatttcc 16380
agcttggcct cgacgcggcg cggtaacata tactgggtgc gcgactcggt aaccggcacg 16440
cgggtacccg tgcgctttcg ccccccgcgg aattagcaca agacaacata cacactgagt 16500
ctcctgctgt tgtgtatccc agcggcgacc gtcagcagcg gcgacatgtc caagcgcaaa 16560
attaaagaag agatgctcca ggtcatcgcg ccggagatct atgggccccc gaagaaggag 16620
gaggatgatt acaagccccg caagctaaag cgggtcaaaa agaaaaagaa agatgatgac 16680
gttgacgagg cggtggagtt tgtccgccgc atggcgccca ggcgccccgt gcagtggaag 16740
ggtcggcgcg tgcagcgagt cctgcgcccc ggcaccgcgg tggtctttac gcccggcgag 16800
cgttccacgc gcactttcaa gcgggtgtac gatgaggtgt acggcgacga ggatctgttg 16860
gagcaggcca accatcgctt tggggagttt gcatatggga aacggccccg cgagagccta 16920
aaagaggacc tgctggcgct accgctggac gagggcaatc ccaccccgag tctgaagccg 16980
gtaaccctgc aacaggtgct gcctttgagc gcgcccagcg agcagaagcg agggttgaag 17040
cgcgagggcg gggacctggc acccaccgtg cagttgatgg tgcccaagcg gcagaagctg 17100
gaggacgtgc tggagaaaat gaaagtagag cccgggatcc agcccgaaat caaggtccgc 17160
cccatcaagc aggtggcgcc cggcgtggga gtccagaccg tggacgttag gattcccacg 17220
gaggagatgg aaacccaaac cgccactccc tcttcggcgg ctagcgccac caccggctcc 17280
gcttcggtag aggtgcagac ggacccctgg ctagccgccg ccgccccggc cgccccccgt 17340
tcgcgcgggc gcaagagaaa ttatccagcg gccagcgcgc tcatgcccca gtacgcactg 17400
catccatcca tcgcgcccac ccccggctac cgcgggtact cgtaccgccc gcgcagatca 17460
gccggcaccc gcggccgccg ccgccgtgcg accacaacca gccgccgccg tcgccgccgc 17520
cgccagccag tgctgacccc cgtgtctgta aggaaggtgg ctcgctcggg gagcacgctg 17580
gtggtgccca gagcgcgcta ccaccccagc attgtttaaa gccggtctct gtatggttct 17640
tgcagatatg gccctcactt gtcgcctccg cttcccggtg ccgggatacc gaggaagaac 17700
tcaccgccgc agaggcatgg cgggcagtgg tctccgcggc ggccgtcgcc atcgccggcg 17760
cgcaaagagc aggcgcatgc gcggcggtgt gctgcccttc ctaatcccgc taatcgccgc 17820
ggcgatcggt gccgtgcccg ggatcgcctc cgtggccctg caggcgtccc agaaacattg 17880
actcttgcaa ccttgcaagc ttgcattttt tggaggaaaa aataaaaagt ctagactctc 17940
acgctcgctt ggtcctgtga ctattttgta gaaaaaagat ggaagacatc aactttgcgt 18000
cgctggcccc gcgtcacggc tcgcgcccgt tcatgggaga ctggacagat atcggcacca 18060
gcaatatgag cggtggcgcc ttcagctggg gcagtctgtg gagtggcctt aaaaattttg 18120
gttccaccat taagaactat ggcaacaaag cgtggaacag cagcacgggt cagatgctga 18180
gagacaagtt gaaagagcag aacttccagg agaaggtggc acagggcctg gcctctggca 18240
tcagcggggt ggtggacata gctaaccagg ccgtgcagaa aaagataaac agtcatctgg 18300
acccccgccc tcaggtggag gaaacgcctc cagccatgga gacggtgtct cccgagggca 18360
aaggcgaaaa gcgcccgcgg cccgacaggg aagagaccct ggtgtcacac accgaggagc 18420
cgccctctta cgaggaggca gtcaaggccg gcctgcccac cactcgcccc atagctccca 18480
tggccaccgg tgtggtgggt cacaggcaac acacccccgc gacactagat ctgcccccgc 18540
cgtccgagcc gactcgccag ccaaaggcgg tgacggtgcc cgctccctcc acttccgccg 18600
ccaacagagt gcctctgcgc cgcgctgcga gcggcccccg ggcctcgcga gtcagcggca 18660
actggcagag cacactgaac agcatcgtgg gcctgggagt gaggagtgtg aagcgccgcc 18720
gttgctactg aatgagcaag ctagctaacg tgttgtatgt gtgtatgcgt cctatgtcgc 18780
cgccagagga gctgttgagc cgccggcgcc gtctgcactc cagcgaattt caagatggcg 18840
accccatcga tgatgcctca gtggtcgtac atgcacatct cgggccagga cgcttcggag 18900
tacctgagcc ccgggctggt gcagttcgcc cgcgccacag acacctactt caacatgagt 18960
aacaagttca ggaaccccac tgtggcgccc acccacgatg tgaccacgga ccggtcgcag 19020
cgcctgacgc tgcggttcat ccccgtggat cgggaggaca ccgcttactc ttacaaggcg 19080
cggttcacgc tggccgtggg cgacaaccgc gtgctggaca tggcctccac ttactttgac 19140
atccgggggg tgctggacag gggccccact ttcaagccct actcgggcac tgcctacaac 19200
tccctggccc ccaagggcgc tcccaattct tgcgagtggg aggaggaaac acaaaatgag 19260
gtacaagcca atgaagaaca actagcagaa gaagaggatg aagaaatggc tcaagaggat 19320
cagcagccta ctaaaaaaac ccatgtatat gctcaggcac ctctttctgg cgaacagatt 19380
accaaagatg gcttgcaaat aggagctgaa gttacaggag aaacatcaaa gcccattttt 19440
gcagacaaga cattccaacc agaacctcag ataggagagt ctcaatggaa tgaggccgat 19500
gctacagtag caggaggtag ggttttgaaa aagactaccc ctatgaaacc ttgctatgga 19560
tcctatgcca gacctaccaa tgccaatgga gggcagggga tacttgaggc aaatgctaaa 19620
ggggaactcg aatctaaagt tgagatgcag tttttctcta acaccacaac tcttaatgta 19680
agagacggtg aaaatggcct taaaccaaaa gtagtgctgt atagcgaaga tgtcaacctg 19740
gaatcccctg acactcatct gtcttacaag cccaaaaaag atgatgttaa tgccaaaatc 19800
atgttgggtc agcaagccat gcccaacaga cccaacctca ttggatttag agataatttc 19860
attgggctca tgtattacaa cagcactgga aacatgggag tgctggcggg tcaggcctct 19920
cagttgaatg ctgtggtgga cttgcaggat agaaacacgg aactgtcata tcagcttatg 19980
cttgattcca ttggagatag aaccagatac ttttccatgt ggaaccaggc agtggatagc 20040
tatgacccag atgttagaat cattgaaaac catggggtgg aggatgagct gcccaactac 20100
tgttttccct tgggcggtat aggaattaca gatacatacc aggccataaa agcagccaat 20160
ggtggagatg ctactacgtg gtctgctgat aacacatttg cagaccgcaa cgaaataggg 20220
gtgggaaaca acttcgccat ggagatcaac atccaggcca acctctggag aaacttcctc 20280
tatgcgaacg tgggactcta cctgccagac aagctcaagt acaaccccac caacgtggac 20340
atctctgaca accccaacac ctatgactac atgaacaagc gggtggtggc ccccggcctg 20400
gtggactgct ttgtcaatgt gggagccagg tggtccctgg actacatgga caacgtcaac 20460
cccttcaacc accaccgcaa tgcgggtctg cgctaccgct ccatgatcct gggcaacggg 20520
cgctatgtgc ccttccacat ccaggtaccc cagaagttct ttgccatcaa gaacctcctg 20580
ctcctgcccg gctcctacac ctacgagtgg aacttcagga aggatgtaaa catggtccta 20640
cagagctctc tgggcaatga ccttagggta gatggggcca gcatcaagtt tgacagcatc 20700
accctctatg ctacattttt ccccatggcc cacaacaccg cctccacgct tgaggccatg 20760
ctgagaaacg acaccaacga ccagtccttc aatgactacc tctctggggc caacatgctc 20820
tacccaatcc cagccaaggc caccaacgtg cccatctcca tcccctctcg caactgggcc 20880
gcctttagag gctgggcctt tacccgcctt aagaccaagg agaccccctc cctgggctcg 20940
ggttttgatc cctactttgt ttactcggga tccatcccct acctggatgg caccttctac 21000
ctcaaccaca ctttcaagaa gatatccatc atgtatgact cctccgtcag ctggccgggc 21060
aacgaccgct tgctcacccc caatgagttc gaggtcaagc gcgccgtgga cggcgagggc 21120
tacaacgtgg cccagtgcaa catgaccaag gactggttcc tggtgcagat gctggccaac 21180
tacaacatag gctaccaggg cttctacatc ccagagagct acaaggacag gatgtattcc 21240
ttcttcagaa atttccaacc catgagccga caggtggtgg acgagaccaa ttacaaggac 21300
tatcaggcca ttggcatcac ccaccagcac aacaactcgg gtttcgtggg ctacctggcg 21360
cccaccatgc gcgagggaca ggcctacccc gccaacttcc cctaccccct gataggcaag 21420
accgcggtcg acagcgtcac ccagaaaaag ttcctctgcg accgcaccct ctggcgcatc 21480
cccttctcta gcaactttat gtccatgggt gcgctcacgg acctgggcca aaacctgctt 21540
tatgccaact ctgcccatgc gctggacatg acttttgagg tggaccccat ggacgagccc 21600
acccttctct atattgtgtt tgaagtgttc gacgtggtca gagtgcacca gccgcaccgc 21660
ggtgtcatcg agaccgtgta cctgcgtacg cccttctcag ccggcaacgc caccacctaa 21720
ggagacagcg ccgccgcctg catgactggt tccaccgagc aagagctcag ggccatcgcc 21780
agagacctgg gatgcggacc ctattttttg ggcacctatg acaaacgctt cccgggtttt 21840
atctcccgag acaagctcgc ctgcgccatc gtcaacacgg ccgcgcgcga gaccgggggc 21900
gtgcactggc tggcctttgg ctgggacccg cgctctaaaa cttgctacct ctttgacccc 21960
tttggcttct ccgatcagcg cctcaggcag atttatgagt ttgagtacga ggggctgttg 22020
cgccgcagcg cgcttgcctc ctcgcccgac cgctgcatca cccttgagaa gtccaccgag 22080
accgtgcagg ggccccactc ggccgcctgc ggtctcttct gttgcatgtt tttgcacgcc 22140
tttgtgcact ggcctcagag tcccatggat cgcaacccca ccatgaactt gctaaaggga 22200
gtgcccaacg ccatgctcca gagcccccag gtcctgccca ccctgcgccg caaccaggaa 22260
cagctctacc gcttcctgga gcgccactcc ccctacttcc gcagccacag cgcgcgcatc 22320
cggggggcca cctctttttg ccacttgcaa gaaaacatgc aagacggaaa atgatgtaca 22380
gcatgctttt aataaatgta aagactgtgc actttattta tacacgggct ctttctggtt 22440
atttattcaa caccgccgtc gccatctaga aatcgaaagg gttctgccgc gcgtcgccgt 22500
gcgccacggg cagagacacg ttgcgatact ggaagcggct cgcccacttg aactcgggca 22560
ccaccatgcg gggcagtggt tcctcgggga aattctcgct ccacagggtg cgggtcagct 22620
gcagcgcgct caggaggtcg ggagccgaga tcttgaagtc gcagttgggg ccggaaccct 22680
gcgcgcgcga gttgcggtac acggggttgc agcactggaa caccagcagg gccggattat 22740
tcacgctggc cagcaggctc tcgtcgctaa tcatgtcgct gtccagatcc tccgcgttgc 22800
tcagggcgaa tggggtcatc ttgcagacct gcctgcccag gaaaggcggg agcccaggct 22860
tgccgttaca gtcgcagcgc aggggcatta gcaggtgccc acggcccgac tgcgcctgcg 22920
ggtacaacgc gcgcatgaag gcttcgatct gcctaaaagc cacctgggtc ttggctccct 22980
ccgaaaagaa catcccacag gacttgctgg agaactggtt cgcgggacag ctggcatcgt 23040
gcaggcagca gcgcgcgtca gtgttggcga tctgcaccac gttgcgaccc caccggtttt 23100
tcactatctt ggccttggaa gcctgctcct ttagcgcgcg ctggccgttc tcgctggtca 23160
catccatctc tatcacctgt tccttgttga tcatgtttgt cccgtgcaga cactttaggt 23220
cgccctccgt ctgggtgcag cggtgctccc acagcgcgca accggtgggc tcccaatttt 23280
tgtgggtcac ccccgcgtag gcctgcaggt aggcctgcag gaagcgcccc atcatggtca 23340
taaaggtctt ctggctcgta aaggtcagct gcaggccgcg atgctcttcg ttcagccagg 23400
tcttgcagat ggcggccagc gcctcggtct gctcgggcag catcttaaaa tttgtcttca 23460
ggtcgttatc cacgtggtac ttgtccatca tggcacgcgc cgcctccatg cccttctccc 23520
aggcggacac catgggcagg cttagggggt ttatcacttc cagcggcgag gacaccgtac 23580
tttcgatttc ttcttcctcc ccctcttccc ggcgcgcgcc cccgctgttg cgcgctctta 23640
ccgcctgcac caaggggtcg tcttcaggca agcgccgcac cgagcgcttg ccgcccttga 23700
cctgcttgat cagtaccggc gggttgctga agcccaccat ggtcagcgcc gcctgctctt 23760
cttcgtcttc gctgtctacc actatttctg gggaggggct tctccgctct gcggcaaagg 23820
cggcggatcg cttctttttt ttcttgggag ccgccgcgat ggagtccgcc acggcgaccg 23880
aggtcgaggg cgtggggctg ggggtgcgcg gcaccagggc ctcgtcgccc tcggactctt 23940
cctctgactc caggcggcgg cggagtcgct tctttggggg cgcgcgcgtt agcggcggcg 24000
gagacgggga cggggacggg gacgggacgc cctccacagg gggcggtctt cgcgcagacc 24060
cgcggccgcg ctcgggggtc ttctcgcgct ggtcttggtc ccgactggcc attgtatcct 24120
cctcctccta ggcagagaga cataaggagt ctatcatgca agtcgagaag gaggagagct 24180
taaccacccc ctctgagacc gccgtcgccg tcgcccccgc taccgccgac gcgcccgcca 24240
caccgagcga cacccccgcg gacccccccg ccgacgcacc cctgttcgag gaagcggccg 24300
tggagcagga cccgggcttt gtctcggcag aggaggattt gcaagaggag gaggataagg 24360
aggagaagcc ctcagtgcca aaagatcata aagagcaaga cgagcacgac gcagacgcac 24420
accagggtga agtcgggcgg ggggacggag ggcatggcgg cgccgactac ctagacgaag 24480
gaaacgacgt gctcttgaag cacctgcatc gtcagtgcgc cattgtctgc gacgctctgc 24540
aggagcgcag cgaggtgccc ctcagcgtgg cggaggtcag ccgcgcctac gagctcagcc 24600
tcttttcccc ccgggtgccc ccccgccgcc gcgaaaacgg cacatgcgag cccaacccgc 24660
gcctcaactt ctaccccgcc tttgtggtgc ccgaggtcct ggccacctat cacatcttct 24720
ttcaaaattg caagatcccc atctcgtgcc gcgccaaccg tagccgcgcc gataagatgc 24780
tggccctgcg ccagggcgac cacatacctg atatcgccgc tttggaagat gtgccaaaga 24840
tcttcgaggg tctgggtcgc aacgagaagc gggcagcaaa ctctctgcaa caggaaaaca 24900
gcgaaaatga gagtcacact ggagcgctgg tggagctgga gggcgacaac gcccgcctgg 24960
cggtgctcaa gcgcagcatc gaggtcaccc actttgccta ccccgcgctc aacctgcccc 25020
ccaaagtcat gaacgcggtc atggacgggc taatcatgcg ccgcggccgg ccccttgctc 25080
cagatgcaaa cttgcatgag gagaccgagg acggtcagcc cgtggtcagc gacgagcagc 25140
tgacgcgctg gctggaaacc gcggaccccg ccgaactgga ggagcggcgc aagatgatga 25200
tggccgcggt gctggtcacc gtagagctgg agtgtctgca gcgcttcttc ggcgaccccg 25260
agatgcagag aaaggtcgag gagaccctac actacacctt ccgccagggc tacgtgcgcc 25320
aggcttgcaa gatctccaac gtggagctca gcaacctggt gtcctacctg ggcatcttgc 25380
atgaaaaccg ccttgggcag agcgtgctac actccaccct gcgcggggag gcgcgccgcg 25440
actacgtgcg cgactgcgtt tacctcttcc tctgctacac ctggcagacg gccatggggg 25500
tctggcagca gtgcctggag gagcgcaacc tcaaggagct ggagaagctc ctgcagcgcg 25560
cgctcaaaga cctctggacg ggctacaacg agcgctcggt ggccgccgcg ctggccgacc 25620
tcatcttccc cgagcgcctg ctcaaaactc tccagcaggg gctgcccgac ttcaccagcc 25680
aaagcatgtt gcaaaatttt aggaacttta tcctggagcg ttctggcatc ctacccgcca 25740
cctgctgcgc cctgcccagc gactttgtcc ccctcgtgta ccgcgagtgc cccccgccgc 25800
tgtggggcca ctgctacctg ttccaactgg ccaactacct gtcctaccac gcggacctca 25860
tggaagactc cagcggcgag gggctcatgg agtgccactg ccgctgcaac ctctgcacgc 25920
cccaccgctc cctggtctgc aacacccaac tgctcagcga gagtcagatt atcggtacct 25980
tcgagctaca gggtccgtcc tcctcagacg agaagtccgc ggctccgggg ctaaaactca 26040
ctccggggct gtggacttcc gcctacctgc gcaaatttgt acctgaagac taccacgccc 26100
acgagatcag gttttacgag gaccaatccc gcccgcccaa ggcggagctg accgcctgcg 26160
tcatcaccca gggcgagatc ctaggccaat tgcaagccat ccaaaaagcc cgccaagagt 26220
ttttgctgag aaagggtcgg ggggtgtatc tggaccccca gtcgggtgag gagctcaacc 26280
cggttccccc gctgccgccg ccgcgggacc ttgcttccca ggataagcat cgccatggct 26340
cccagaaaga agcagcagcg gccgccactg ccgccacccc acacgctgga ggaagaggag 26400
gaatactggg acagtcaggc agaggaggtt tcggacgagg aggagccgga gacggagatg 26460
gaagagtggg aggaggacag cttagacgag gaggcttccg aagccgaaga ggcaggcgca 26520
acaccgtcac cctcggccgc agccccctcg caggcgcccc cgaagtccgc tcccagcatc 26580
agcagcaaca gcagcgctat aacctccgct cctccaccgc cgcgacccac ggccgaccgc 26640
agacccaacc gtagatggga caccaccgga accggggccg gtaagtcctc cgggaaaggc 26700
aagcaagcgc agcgccaagg ctaccgctcg tggcgcgctc acaagaacgc catagtcgct 26760
tgcttgcaag actgcggggg gaacatctcc ttcgcccgcc gcttcctgct cttccaccac 26820
ggtgtggcct tcccccgtaa cgtcctgcat tactaccgtc atctctacag cccctactgc 26880
ggcggcagtg agccagaggc ggccggcggc agcggcgccc gtttcggtgc ctaggaagac 26940
ccagggcaag acttcagcca agaaactcgc ggcggccgcg gcgaacgcgg tcgcgggggc 27000
cctgcgcctg acggtgaacg aacccctgtc gacccgcgaa ctgaggaacc gaatcttccc 27060
cactctctat gccatcttcc agcagagcag agggcaggat caggaactga aagtaaaaaa 27120
caggtctctg cgctccctca cccgcagctg tctgtatcac aagagcgaag accagcttcg 27180
gcgcacgctg gaggacgctg aggcactctt cagcaaatac tgcgcgctca ctcttaagga 27240
ctagctccgc gcccttctcg aatttaggcg ggaacgccta cgtcatcgca gcgccgccgt 27300
catgagcaag gacattccca cgccatacat gtggagctat cagccgcaga tgggactcgc 27360
ggcgggcgcc tcccaggatt actccacccg catgaactgg ctcagtgccg gcccacacat 27420
gatctcacag gttaatgaca tccgcaccca tcgaaaccaa atattggtgg agcaggcggc 27480
aattaccacc acgccccgca ataatcccaa ccccagggag tggcccgcgt ccctggtgta 27540
tcaggaaatt cccggcccca ccaccgtact acttccgcgt gattcccagg ccgaagtcca 27600
aatgactaac tcaggggcac agctcgcggg cggctgtcgt cacagggtgc ggcctcctcg 27660
ccagggtata actcacctga agatccgagg cagaggtatt cagctcaacg acgagtcggt 27720
gagctcctcg ctcggtctca gacctgacgg gaccttccag atagccggag ccggccgatc 27780
ttccttcacg ccccgccagg cgtacctgac tctgcagagc tcgtcctcgg cgccgcgctc 27840
gggcggcatc gggactctcc agttcgtgca ggagtttgtg ccctcggtct acttcaaccc 27900
cttctcgggc tctcccggtc gctacccgga ccagttcatc ccgaactttg acgccgcgag 27960
ggactcggtg gacggctacg actgaatgtc gggtggaccc ggtgcagagc aacttcgcct 28020
gaagcacctt gaccactgcc gccgccctca gtgctttgcc cgctgtcaga ccggtgagtt 28080
ccagtacttt tccctgcccg actcgcaccc ggacggcccg gcgcacgggg tgcgcttttt 28140
catcccgagt caggtccgct ctaccctaat cagggagttc accgcccgtc ccctactggc 28200
ggagttggaa aaggggcctt ctatcctaac cattgcctgc atctgctcta accctggatt 28260
acaccaagat ctttgctgtc atttgtgtgc tgagtataat aaaggctgag atcagaatct 28320
actcgggctc ctgtcgccat cctgtcaacg ccaccgtcca agcccggccc gatcagcccg 28380
aggtgaacct cacctgcggt ctgcaccggc gcctgaggaa atacctagct tggtactaca 28440
acagcactcc ctttgtggtt tacaacagct ttgaccagga cggggtctca ctgagggata 28500
acctctcgaa cctgagctac tccatcagga agaacaacac cctcgagcta cttcctcctt 28560
acctgcccgg gacttaccag tgtgtcaccg gtccctgcac ccacacccac ctgttgatcg 28620
taaacgactc tcttccgaga acagacctca ataactcctc tccgcagttc cccagaacag 28680
gaggtgagct caggaaaccc cgggtaaaga agggtggaca agagttaaca cttgtggggt 28740
ttctggtgta tgtgacgctg gtggtggctc ttttgattaa ggcttttcct tccatgtctg 28800
aactatccct cttcttttat gaacaactcg actagtgcta acgggaccct acccaacgaa 28860
tcgggattga atatcggtaa ccaggttgca gtttcacttt tgattacctt catagtcctc 28920
ttcctgctag tgctgtcgct tctgtgcctg cggatcgggg gctgctgcat ccacgtttat 28980
atctggtgct ggctgtttag aaggttcgga gaccaccgca ggtagaataa acaaacctag 29040
acctagaaat ggacggtctc tgcagcgagc aacgcatact agagaggcgc cggcaaaaag 29100
cagagctcga gcgtcttaaa caagagctcc aagacgccgt ggccatacac cagtgcaaaa 29160
aagggctctt ctgtctggta aaacaggcca cgctcaccta tgaaaaaaca ggtgacaccc 29220
accgcctagg atacaagctg cccacacagc gccaaaagtt tgcccttatg ataggtgaac 29280
aacccatcac cgtgacccag cactccgtgg agacagaagg ctgcattcat gctccctgca 29340
ggggcgctga ctgcctctac accttgatca aaaccctttg cggtctcaga gaccttatcc 29400
ctttcaattg atcataactg taatcaataa aaaatcactt acttgaaatc tgatagcaag 29460
cctctgtcca attttttcag caacacttcc ttcccctctt cccaactctg gtactctagg 29520
cgcctcctag ctgcaaactt cctccacagt ctgaagggaa tgtcagattc ctcctcctcc 29580
tgtccctccg cacccacgat cttcatgttg ttgcagatga agcgcaccaa aacgtctgac 29640
gagagcttca accccgtgta cccctatgac acggaaaacg gtcctccctc cgtccctttc 29700
ctcacccctc ccttcgtgtc tcccgatgga ttccaagaga gcccccccgg ggtcctgtct 29760
ctgaacctgg ccgagcccct ggtcacttcc cacggcatgc tcgccctgaa aatgggaagt 29820
ggcctctccc tggacgacgc cggcaacctc acctctcaag atgtcaccac cactacccct 29880
cccctgaaaa aaaccaagac caacctcagc ctagaaacct cagcccccct gactgtgagc 29940
acctcaggcg ccctcaccct agcagccgcc gttcccctgg cggtggccgg cacctccctc 30000
accatgcaat cagaggcccc cctgacagtc caagatgcaa aactcaccct ggccaccaag 30060
ggccccctga ccgtgtctga aggcaaactg gccttgcaga cctcggcccc gctgacggcc 30120
gctgacagca gcgccctcac cgttagcgcc acaccaccca tcagtgtaag cagtggaagt 30180
ttgggcttag acatggaaga ccccatgtat actcatgatg gaaaactggg aataagaatt 30240
ggaggcccac tgagagtagt agacagcctg cacacactga ctgtagttac cggaaatgga 30300
atagctgtag ataacaatgc cctccaaact agagttacgg gcgccctggg ttatgacaca 30360
tcaggaaacc tacaactgag agccgcgggg ggtatgcgaa ttgatgcaaa tggccaactt 30420
atccttgatg tggcataccc atttgatgct caaaacaatc tcagccttag acttggtcag 30480
ggacccctgt atgtaaacac agaccacaac ctagatttga attgcaacag aggtctgacc 30540
acaactacca ccaacaacac aaaaaaactt gaaactaaaa ttggctcagg cttagactat 30600
gataccaatg gtgctgtcat tattaaactt ggtactggtg taagctttga cagcacaggc 30660
gccctaactg tgggaaacac tggcgatgat aaactgactc tgtggacaac cccagaccca 30720
tctccaaatt gcagaattca cgcagacaaa gactgcaagt ttactctagt cctaactaag 30780
tgtggaagtc aaatcctggc ttctgtcgcc gccctagcgg tgtcaggaaa tctggcttca 30840
ataacaggca ccgttgccag cgttaccatc tttctcagat ttgatcagaa tggagtgctt 30900
atggaaaact cctccctaga caagcagtac tggaacttca gaaatggtaa ctcaaccaat 30960
gccaccccct acaccaatgc agttgggttc atgccaaacc tcgcagcata ccccaagaca 31020
cagagccaga ctgctaaaaa caacattgta agtcaggttt acttgaatgg ggacaaatcc 31080
aaacccatga cccttaccat taccctcaat ggaactaatg aatccagtga aactagccag 31140
gtgagtcact actccatgtc atttacatgg gcttgggaga gtgggcaata tgccaccgaa 31200
acctttgcca ccaattcctt taccttctct tacattgctg aacaataaag aaagcacaga 31260
gatgcttgtt tttgatttca aaattgtgtg cttttattta ttttcaagct tacagtattt 31320
ccagtagtca ttcgaataga gcttaatgaa actgcatgag aacccttcca catagcttaa 31380
attatcacca gtgcaaatgg agaaaaatca acataccttt ttatccagat atcacagaac 31440
cctagtattc aacctgccac ctccctccca acacacagag tacacagtcc tttctccccg 31500
gctggcctta aaaagcatca tatcatgggt aacagacata ttcttaggtg ttatattcca 31560
cacggtttcc tgtcgagcca aacgctcatc agtgatatta ataaactccc cgggcagctc 31620
acttaagttc atgtcgctgt ccagctgctg agccacaggc tgctgtccaa cttgcggttg 31680
cttaacgggc ggcgaaggag aagtccacgc ctacatgggg gtagagtcat aatcgtgcat 31740
caggataggg cggtggtgct gcagcagcgc gcgaataaac tgctgccgcc gccgctccgt 31800
cctgcaggaa tacaacatgg cagtggtctc ctcagcgatg attcgcaccg cccgcagcat 31860
aaggcgcctt gtcctccggg cacagcagcg caccctgatc tcacttaaat cagcacagta 31920
actgcagcac agcaccacaa tattgttcaa aatcccacag tgcaaggcgc tgtatccaaa 31980
gctcatggcg gggaccacag aacccacgtg gccatcatac cacaagcgca ggtagattaa 32040
gtggcgaccc ctcataaaca cgctggacat aaacattacc tcttttggca tgttgtaatt 32100
caccacctcc cggtaccata taaacctctg attaaacatg gcgccatcca ccaccatcct 32160
aaaccagctg gccaaaacct gcccgccggc tatacactgc agggaaccgg gactggaaca 32220
atgacagtgg agagcccagg actcgtaacc atggatcatc atgctcgtca tgatatcaat 32280
gttggcacaa cacaggcaca cgtgcataca cttcctcagg attacaagct cctcccgcgt 32340
tagaaccata tcccagggaa caacccattc ctgaatcagc gtaaatccca cactgcaggg 32400
aagacctcgc acgtaactca cgttgtgcat tgtcaaagtg ttacattcgg gcagcagcgg 32460
atgatcctcc agtatggtag cgcgggtttc tgtctcaaaa ggaggtagac gatccctact 32520
gtacggagtg cgccgagaca accgagatcg tgttggtcgt agtgtcatgc caaatggaac 32580
gccggacgta gtcatatttc ctccagcaga accaagtgcg cgcgtggcag ctatccttgc 32640
gtcttctgtc tcgccgcctg ccccgctcgg tgtagtagtt gtaatacagc cactccctca 32700
gaccgtcaag gcgctccctg gcgtccggat ctataacaac accatcctgc agcgccgccc 32760
tgatgacatc caccaccgta gagtatgcca agcccagcca ggaaatgcac tcactttgac 32820
agcgagagat aggaggagcg ggaagagatg gaagaaccat gatagtaaaa gaacttttat 32880
tccaatcgat cctctacaat gtcaaagtgt agatctatca gatggcactg gtctcctccg 32940
ctgagtcgat caaaaataac agctaaacca caaacaacac gattggtcaa atgctgcaca 33000
agggcttgca gcataaaatc gcctcgaaag tccaccgcaa gcataacatc aaagccaccg 33060
cccctatcat gatctatgat aaaaacccca cagctatcca ccagacccat atagttttca 33120
tctctccatc gtgaaaaaat atttacaagc tcctccttta aatcacctcc aaccaattca 33180
aaaagttgag ccagaccgcc ctccaccttc attttcagca tgcgcatcat gattgcaaaa 33240
attcaggctc ctcagacacc tgtataagat tgagaagcgg aacattaaca tcaatgtttc 33300
gctcgcgaag atcgcgcctc agtgcaagca tgatataatc ccacaggtcg gagcggatca 33360
gcgaggacat ctccccgcca ggaaccaact caacggagcc tatgctgatt ataatacgca 33420
tattcggggc tatgctaacc agcacggccc ccaaataggc gtactgcata ggcggcgaca 33480
aaaagtgaac agtttgggtt aaaaaatcag gcaaacactc gcgcaaaaaa gcaagaacat 33540
cataaccatg ctcatgcaaa tagatgcaag taagctcagg aacgaccaca gaaaaatgca 33600
caatttttct ctcaaacatg actgcgagcc ctgcaaaaaa taaaaaagaa acattacaca 33660
agagtagcct gtcttacaat gggatagact actctaacca acataagacg ggccacgaca 33720
tcgcccgcgt ggccataaaa aaaattatcc gtgtgattaa aaagaagcac agatagctgg 33780
ccagtcatat ccggagtcat cacgtgcgaa cccgtgtaga cccccgggtt ggacacatcg 33840
gccaaagaaa gaaagcggcc aatgtatccc ggaggaatga taacactaag acgaagatac 33900
aacagaataa ccccatgggg gggaataaca aagttagtag gtgaataaaa acgataaaca 33960
cccgaaactc cctcctgcgt aggcaaaata gcgccctccc cttccaaaac aacatacagc 34020
gcttccacag cagccatgac aaaagactca aaacactcaa aagactcagt cttaccagga 34080
aaataaaagc actctcacag caccagcact aatcagagtg tgaagagggc caagtgccga 34140
acgagtatat ataggaatta aaaatgacgt aaatgtgtaa aggtcaaaaa acgcccagaa 34200
aaatacacag accaacgccc gaaacgaaaa cccgcgaaaa aatacccaga agttcctcaa 34260
caaccgccac ttccgctttc ccacgatacg tcacttcctc gaaaatagca aactacattt 34320
cccacatgta caaaaccgaa accactcccc ttgtcaccgc ccacaactta catcttaatt 34380
aacaaacgtc aaagcctacg tcagccgccc cgcctcgccc cgcccacctc attatcatat 34440
tggccacaat ccaaaataag gtatattatt gatgatg 34477
<210> 18
<211> 35469
<212> DNA
<213> 人工序列
<220>
<223> BZ1/BZ28F.RSVF-2A-GLuc腺病毒载体
<400> 18
catcatcaat aatatacctt attttggatt gtggccaata tgataatgag gtgggcgggg 60
cgaggcgggg cgggtgacgt aggacgtgcg agtagggttg ggaggtgtgg cggaagtgtg 120
gcatttgtaa gtgggaggag cttacatgca agcttccgtc gcggaaaatg tgacgttttt 180
tatgagcgcc gcctacctcc ggaagtgcca attttcgcgc gctttttacc ggatatcgta 240
gtaattttgg gcgggaccat gtaagatttg gccattttcg cgcgaaaagt gaaacgggga 300
agtgaaaact gaataatagg gcgttagtca tagcgcgtaa tatttaccga gggccgaggg 360
actttgaccg attacgtgga ggactcgccc aggtgttttt tacgtgaatt tccgcgttcc 420
gggtcaaagt ctccgttttt attgtcaccg tcatttgacg cggagggtat ttaaacccgc 480
tgcgctccta gcgatcgctc aatattggcc attagccata ttattcattg gttatatagc 540
ataaatcaat attggctatt ggccattgca tacgttgtat ccatatcata atatgtacat 600
ttatattggc tcatgtccaa cattaccgcc atgttgacat tgattattga ctagttatta 660
atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata 720
acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat 780
aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga 840
gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc 900
ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt 960
atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat 1020
gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag 1080
tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc 1140
aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga 1200
ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg 1260
ctgttttgac ctccatagaa gacaccggga ccgatccagc ctccgcggtt ggccgtgctc 1320
ttcctgacgg gtaggtgtcc cctaacctag ggagccaacc atcggggggc cttctcccta 1380
aatccccgtg gcccaccctc ctgggcagag gcagcaggtt tctcactggc cccctctccc 1440
ccacctccaa gcttggcctt tcggctcaga tctcagccca cagctggcct gatctgggtc 1500
tcccctccca ccctcaggga gccaggctcg gcatttcgtc gacatggaac tgctgatcct 1560
gaaggccaac gccatcacca ccatcctgac cgccgtgacc ttctgcttcg ccagcggcca 1620
gaacatcacc gaggaattct accagagcac ctgtagcgcc gtgtccaagg gctacctgag 1680
cgccctgcgg accggctggt acaccagcgt gatcaccatc gagctgagca acatcaaaaa 1740
gaacaagtgc aacggcaccg acgccaaaat caagctgatc aagcaggaac tggacaagta 1800
caagaacgcc gtgaccgagc tgcagctgct gatgcagagc acccccgcca ccaacaaccg 1860
ggccagacgg gagctgcccc ggttcatgaa ctacaccctg aacaacgcca aaaagaccaa 1920
cgtgaccctg agcaagaagc ggaagcggcg gttcctgggc ttcctgctgg gcgtgggcag 1980
cgccattgct agcggagtgg ctgtgtctaa ggtgctgcac ctggaaggcg aagtgaacaa 2040
gatcaagtcc gccctgctga gcaccaacaa ggccgtggtg tccctgagca acggcgtgtc 2100
cgtgctgacc agcaaggtgc tggatctgaa gaactacatc gacaagcagc tgctgcccat 2160
cgtgaacaag cagagctgca gcatcagcaa catcgagaca gtgatcgagt tccagcagaa 2220
gaacaaccgg ctgctggaaa tcacccgcga gttcagcgtg aacgccggcg tgaccacccc 2280
cgtgtccacc tacatgctga ccaacagcga gctgctgagc ctgatcaacg acatgcccat 2340
caccaacgac cagaaaaagc tgatgagcaa caacgtgcag atcgtgcggc agcagagcta 2400
ctccatcatg tccatcatca aagaagaggt gctggcctac gtggtgcagc tgcccctgta 2460
cggcgtgatc gacaccccct gctggaagct gcacaccagc cccctgtgca ccaccaacac 2520
caaagagggc agcaacatct gcctgacccg gaccgaccgg ggctggtact gcgataatgc 2580
cggcagcgtg tcattctttc cacaagccga gacatgcaag gtgcagagca accgggtgtt 2640
ctgcgacacc atgaacagcc tgaccctgcc cagcgaggtg aacctgtgca acgtggacat 2700
cttcaaccct aagtacgact gcaagatcat gacctccaag accgacgtgt ccagctccgt 2760
gatcacctcc ctgggcgcca tcgtgtcctg ctacggcaag accaagtgca ccgccagcaa 2820
caagaaccgg ggcatcatca agaccttcag caacggctgc gactacgtgt ccaacaaggg 2880
cgtggacacc gtgtccgtgg gcaacaccct gtactacgtg aacaaacagg aaggcaagag 2940
cctgtacgtg aagggcgagc ccatcatcaa cttctacgac cccctggtgt tccccagcga 3000
cgagttcgac gccagcatca gccaggtcaa cgagaagatc aaccagagcc tggccttcat 3060
cagaaagagc gacgagctgc tgcacaatgt gaatgccgtg aagtccacca ccaatatcat 3120
gatcaccaca atcatcatcg tgatcatcgt catcctgctg tccctgatcg ccgtgggcct 3180
gctgctgtac tgcaaggccc ggtccacccc tgtgaccctg tccaaggacc agctgagcgg 3240
catcaacaat atcgccttct ccaacggacg cgtgaccgag ctgctttacc ggatgaagcg 3300
ggctgagaca tattgcccga gacccctgtt ggcaatccat cctactgagg ctcgccacaa 3360
acagaaaatc gtggcccccg tcaaacagac actcaatttt gacttgttga aacttgcagg 3420
agatgttgag tcaaaccccg ggcctatggg cgtcaaggtc ctgttcgctc tgatttgtat 3480
cgctgtcgct gaagctaagc caaccgagaa taatgaagac tttaatatcg tggccgtggc 3540
ttctaacttc gctaccacag acctggatgc agacagggga aagctgccag gcaagaaact 3600
gcccctggag gtcctgaagg agatggaagc aaatgcccgg aaagccgggt gcacaagagg 3660
atgcctgatt tgtctgagcc acatcaagtg cactcctaag atgaagaagt tcatccccgg 3720
ccggtgccat acctacgagg gcgataagga atccgcccag ggaggaatcg gagaggctat 3780
cgtggatatt cccgaaatcc ctggcttcaa agacctggag cccatggaac agtttattgc 3840
acaggtggat ctgtgcgtcg actgtactac cggatgcctg aagggactgg caaacgtcca 3900
gtgtagcgac ctgctgaaga aatggctgcc tcagcgatgt gctacatttg ccagcaagat 3960
tcagggccag gtggacaaga ttaagggagc aggaggcgac tgataattct agacgagatc 4020
cgaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 4080
caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 4140
cttatcatgt ctgcgatcgc tgatgagacc aggaccaggt gccgaccctg cgagtgcggc 4200
ggcaagcaca tgagaaatca gcctgtgatg ttggatgtga ccgaggagct taggcctgac 4260
catctggtgc tggcctgcac cagggccgag tttgggtcta gcgatgagga taccgattga 4320
ggtgggtaag gtgggcgtgg ctagcagggt gggcgtgtat aaattggggg tctaaggggt 4380
ctctctgttt gtcttgcaac agccgccgcc atgagcgaca ccggcaacag ctttgatgga 4440
agcatcttta gcccctatct gacagtgcgc atgcctcact gggccggagt gcgtcagaat 4500
gtgatgggtt ccaacgtgga tggacgtccc gttctgcctt caaattcgtc tacgatggcc 4560
tacgcgaccg tgggaggaac tccgctggac gccgcgacct ccgccgccgc ctccgccgcc 4620
gccgcgaccg cgcgcagcat ggctacggac ctttacagct ctttggtggc gagcagcgcg 4680
gcctctcgcg cgtctgctcg ggatgaaaaa ctgactgctc tgctgcttaa actggaagac 4740
ttgacccggg agctgggtca actgacccag caggtctcca gcttgcgtga gagcagcctt 4800
gcctccccct aatggcccat aatataaata aaagccagtc tgtttggatt aagcaagtgt 4860
atgttcttta tttaactctc cgcgcgcggt aagcccggga ccagcggtct cggtcgttta 4920
gggtgcggtg gattctttcc aacacgtggt acaggtggct ctggatgttt agatacatgg 4980
gcatgagtcc atccctgggg tggaggtagc accactgcag agcttcgtgc tcgggggtgg 5040
tgttgtatat gatccagtcg tagcaggagc gctgggcgtg gtgctgaaaa atgtccttaa 5100
gcaagaggct tatagctagg gggaggccct tggtgtaagt gtttacaaat ctgcttagct 5160
gggaggggtg catccggggg gatatgatgt gcatcttgga ctggattttt aggttggcta 5220
tgttcccacc cagatccctt ctgggattca tgttgtgcag gaccaccagc acggtatatc 5280
cagtgcactt gggaaattta tcgtggagct tagacgggaa tgcatggaag aacttggaga 5340
cgcccttgtg gcctcccaga ttttccatac attcgtccat gatgatggca atgggcccgt 5400
gggaagctgc ctgagcaaaa acgtttctgg catcgctcac atcgtagtta tgttccaggg 5460
tgaggtcatc ataggacatc tttacaaatc gggggcggag ggtcccggac tgggggatga 5520
tggtaccctc gggccccggg gcgtagttcc cctcacagat ctgcatctcc caggctttca 5580
tttcagaggg agggatcata tccacctgcg gggcgatgaa aaagacagtt tctggcgcag 5640
gggagattaa ctgggatgag agcaggtttc tgagcagctg tgactttcca cagccggtgg 5700
gcccatatat cacgcctatc accggctgca gctggtagtt aagagagctg cagctgccgt 5760
cctcccggag caggggggcc acctcgttga gcatatccct gacgtggatg ttctccctga 5820
ccagttccgc cagaaggcgc tcgccgccca gcgaaagcag ctcttgcaag gaagcaaaat 5880
ttttcagcgg tttcaggcca tcggccgtgg gcatgttttt cagcgtctgg gtcagcagct 5940
ccagcctgtc ccagagctcg gtgatgtgct ctacggcatc tcgatccagc agatctcctc 6000
gtttcgcggg ttggggcggc tttcgctgta gggcaccagc cgatgggcgt ccagcggggc 6060
cagagtcatg tccttccatg ggcgcagagt cctcgtcagg gtggtctggg tcacggtgaa 6120
ggggtgcgct ccgggttggg cactggccag ggtgcgcttg aggctggttc tgctggtgct 6180
gaatcgctgc cgctcttcgc cctgcgcgtc ggccaggtag catttgacca tggtctcgta 6240
gtcgagaccc tcggcggcgt gccccttggc gcggagcttt cccttggagg tggcgccgca 6300
cgaggggcac tgcaggctct tcagggcgta gagcttggga gcgagaaaca cggactctgg 6360
ggagtaggcg tccgcgccgc aggccgcgca gaccgtctcg cattccacca gccaagtgag 6420
ttccgggcgg tcagggtcaa aaaccaggtt gcccccatgc tttttgatgc gtttcttacc 6480
tcggctctcc atgaggcggt gtcccttctc ggtgacgaag aggctgtccg tgtccccgta 6540
gaccgacttc aggggcctgt cttccagcgg agtgcctctg tcctcctcgt agagaaactc 6600
tgaccactct gagacgaagg cccgtgtcca ggccaggacg aaggaggcca cgtgggaggg 6660
gtagcggtcg ttgtccacta gcgggtccac cttctccagg gtgtgcagac acatgtcccc 6720
ctcctccgcg tccagaaaag tgattggctt ataggtgtag gacacgtgac cgggggttcc 6780
cgacgggggg gtataaaagg gggtgggcgc cctttcatct tcactctctt ccgcatcgct 6840
gtctgcgaga gccagctgct ggggtaagta ttccctttca aaggcgggca tgacctcagc 6900
gctcaggttg tcagtttcta aaaatgagga ggatttgatg ttcacctgtc cggaagtgat 6960
acctttgagg gtacctgggt ccatctggtc agaaaacact atttttttgt tgtcaagctt 7020
ggtggcgaac gacccgtaga gggcgttgga gagcagcttg gcaatggagc gcagggtctg 7080
gtttttgtcg cggtcggctc gctccttggc cgcgatgttg agttgcacgt attcgcgggc 7140
cacgcacttc cactcgggga agacggtggt gcgctcgtct gggatcaggc gcaccctcca 7200
gccgcggttg tgcagggtga ccatgtcgac gctggtggcg acctcaccgc gcagacgctc 7260
gttggtccag cagaggcggc cgcctttgcg cgagcagaag gggggtaggg ggtccagctg 7320
gtcctcgttt ggggggtccg cgtcgatggt aaagaccccg gggagcaggc gcgggtcaaa 7380
gtagtcgatc ttgcaagctt gcatgtccag agcccgctgc cattcgcggg cggcgagcgc 7440
gcgctcgtag gggttgaggg gcgggcccca gggcatgggg tgggtgagcg cggaggcgta 7500
catgccgcag atgtcataca cgtacagggg ttccctgagg ataccgaggt aggtggggta 7560
gcagcgcccc ccgcggatgc tggcgcgcac gtagtcatag agctcgtggg agggggccag 7620
catgttgagc ccaaggttgg tgcgctgggg gcgctcggcg cggaagacga tctgtctgaa 7680
gatggcatgg gagttggagg agatggtggg tcgctggaag acgttgaagc ttgcttcttg 7740
caagcccacg gagtccctga cgaaggaggc gtaggactcg cgcagcttgt gcaccagctc 7800
ggcggtgacc tggacgtcga gcgcgcagta gtcgagggtc tcgcggatga tgtcatactt 7860
atcctccccc ttctttttcc acagctcgcg gttgaggacg aactcttcgc ggtctttcca 7920
gtactcttgg aggggaaacc cgtccgtgtc cgaacggtaa gagcctagca tgtagaactg 7980
gttgacggcc tggtaggggc agcagccctt ctccacgggc agcgcgtagg cctgcgccgc 8040
cttgcggagg gaggtgtggg taagggcgaa agtgtccctg accatgactt tgaggtattg 8100
atgtctgaag tctgtgtcat cgcagccgcc ctgttcccac agggtgtagt ccgtgcgctt 8160
tttggagcgc gggttgggca gggagaaggt gaggtcattg aagaggatct tccccgctcg 8220
aggcatgaag tttctggtga tgcgaaaggg ccctgggacc gaggagcggt tgttgatgac 8280
ctgggcggcc aggacgatct cgtcaaagcc gtttatgttg tggcccacga tgtagagctc 8340
caggaagcgg ggctggccct tgatggaggg gagcttttta agttcctcgt aggtaagctc 8400
ctcgggcgat tccaggccgt gctcctccag ggcccagtct tgcaagtgag ggttggccgc 8460
caggaaggat cgccagaggt cgcgggccat gagggtctgc aggcggtcgc ggaaggttct 8520
gaactgccgc cctacggcca tcttttcggg ggtgatgcag tagaaggtga gggggtcttt 8580
ctcccagggg tcccatctga gctcttgggc gaggtcgcgc gcggcggcga ccagagcctc 8640
gtcgcccccc agtttcatga ccagcatgaa gggcacgagc tgcttgccaa aggctcccat 8700
ccaagtgtag gtctctacat cgtaggtgac aaagaggcgc tccgtgcgag gatgagagcc 8760
gatcgggaag aactggatct cccgccacca gttggaggat tggctgttga tgtggtgaaa 8820
gtagaagtcc cgtctgcggg ccgagcactc gtgctggctt ttgtaaaagc gaccgcagta 8880
ctggcagcgc tgcacgggtt gtatatcttg cacgaggtga acctggcgac ctctgacgag 8940
gaagcgcagc gggaatctaa gtcccccgcc tggggtcccg tgtggctggt ggtcttctac 9000
tttggttgtc tggccgccag catctgtctc ctggagggcg atggtggagc agaccaccac 9060
gccgcgagag ccgcaggtcc agatctcggc gctcggcggg cggagtttga tgacgacatc 9120
gcgcacattg gagctgtcca tggtctccag ctcccgcggc ggcaggtcag ccgggagttc 9180
ctggaggttc acctcgcaga gacgggtcaa ggcgcggaca gtgttgagat ggtatctgat 9240
ttcaaggggc gtgttggagg cggagtcgat ggcttgcaga aggccgcagc cccggggggc 9300
cacgatggtt ccccgcgggg cgcgagggga ggcggaagct gggggtgtgt tcagaagcgg 9360
tgacgcgggc gggcccccgg aggtaggggg ggttccggcc ccacaggcat gggcggcagg 9420
ggcacgtctt cgccgcgcgc gggcaggggc tggtgctggc tccggagagc gcttgcgtgc 9480
gcgacgacgc gacggttggt gtcctgtatc tggcgcctct gagtgaagac cacgggtccc 9540
gtgaccttga acctgaaaga gagttcgaca gaatcaatct cggcatcgtt gacagcggcc 9600
tggcgcagga tctcctgcac gtcgcccgag ttgtcctggt aggcgatctc tgccatgaac 9660
tgctcgatct cttcctcctg gagatctcct cgtccggcgc gctccacggt ggccgccagg 9720
tcgttggaga tgcgacccat gagctgcgag aaggcgttga ggccgccctc gttccagacc 9780
cggctgtaga ccacgccccc ctcggcgtcg cgggcgcgca tgaccacctg ggccaggttg 9840
agctccacgt gtcgcgtgaa gacggcgtag ttgcgcaggc gctggaaaag gtagttcagg 9900
gtggtggcgg tgtgttcggc gacgaagaag tacatgaccc agcgccgcaa cgtggattca 9960
ttgatgtccc ccaaggcctc caggcgctcc atggcctcgt agaagtccac ggcgaagttg 10020
aaaaactggg agttgcgagc ggacacggtc aactcctcct ccagaagacg gatgagctcg 10080
gcgacagtgt cgcgcacctc gcgctcgaag gccacggggg gcgcttcttc ctcttccacc 10140
tcttcttcca tgattgcttc ttcttcctca gccgggacgg gagggggcgg cggcggggga 10200
ggggcgcggc ggcggcggcg gcgcaccggc aggcggtcga tgaagcgctc gatcatctcc 10260
ccccgcatgc ggcgcatggt ctcggtgacg gcgcggccgt tctcccgggg gcgcagctca 10320
aagacgccgc ctctcatctc gccgcggggc gggcggccgt gaggtagcga gacggcgctg 10380
actatgcatc ttaacaattg ctgtgtaggt acgccgccaa gggacctgat tgagtccaga 10440
tccaccggat ccgaaaacct ttggaggaaa gcgtctatcc agtcgcagtc gcaaggtagg 10500
ctgagcaccg tggcgggcgg gggcgggtcg ggagagttcc tggcggagat gctgctgatg 10560
atgtaattaa agtaggcggt cttgagaagg cggatggtgg acaggagcac catgtctttg 10620
ggtccggcct gttggatgcg gaggcggtcg gccatgcccc aggcctcgtt ctgacaccgg 10680
cgcaggtctt tgtagtagtc ttgcatgagt ctttccaccg gcacctcttc tccttcctct 10740
tctccatctc gccggtggtt tctcgcgccg cccatgcgcg tgaccccaaa gcccctgagc 10800
ggctgcagca gggccaggtc ggcgaccacg cgctcggcca agatggcctg ctgtacctga 10860
gtgagggtcc tctcgaagtc atccatgtcc acgaagcggt ggtaggcgcc cgtgttgatg 10920
gtgtaggtgc agttggccat gacggaccag ttgacggtct ggtgtcccgg ctgcgagagc 10980
tccgtgtacc gcaggcgcga gaaggcgcgg gaatcgaaca cgtagtcgtt gcaagtccgc 11040
accagatact ggtagcccac caggaagtgc ggcggaggtt ggcgatagag gggccagcgc 11100
tgggtggcgg gggcgccggg cgccaggtct tccagcatga ggcggtggta tccgtagatg 11160
tacctggaca tccaggtgat gccggcggcg gtggtggtgg cgcgcgcgta gtcgcggacc 11220
cggttccaga tgtttcgcag gggcgagaag tgttccatgg tcggcacgct ctggccggtg 11280
aggcgcgcgc agtcgttgac gctctataca cacacaaaaa cgaaagcgtt tacagggctt 11340
tcgttctgta gcctggagga aagtaaatgg gttgggttgc ggtgtgcccc ggttcgagac 11400
caagctgagc tcggccggct gaagccgcag ctaacgtggt attggcagtc ccgtctcgac 11460
ccaggccctg tatcctccag gatacggtcg agagcccttt tgctttcttg gccaagcgcc 11520
cgtggcgcga tctgggatag atggtcgcga tgagaggaca aaagcggctc gcttccgtag 11580
tctggagaaa caatcgccag ggttgcgttg cggcgtaccc cggttcgagc ccctatggcg 11640
gcttggatcg gccggaaccg cggctaacgt gggctgtggc agccccgtcc tcaggacccc 11700
gccagccgac ttctccagtt acgggagcga gccccttttg ttttttattt tttagatgca 11760
tcccgtgctg cggcagatgc gcccctcgcc ccggcccgat cagcagcagc aacagcaggc 11820
atgcagaccc ccctctcctc tacccgcccc ggtcaccacg gccgcggcgg ccgtgtccgg 11880
cgcggggggc gcgctggagt cagatgagcc accgcggcgg cgacctaggc agtatctgga 11940
cttggaagag ggcgagggac tggcgcggct gggggcgagc tctccagagc gccacccgcg 12000
ggtgcagttg aaaagggacg cgcgcgaggc gtacctgccg cggcagaacc tgtttcgcga 12060
ccgcgggggc gaggagcccg aggagatgcg agactgcagg ttccaagcgg ggcgcgagct 12120
gcgccgcggg ttggacagac agcgcctgct gcgcgaggag gactttgagc ccgacacgca 12180
gacgggcatc agccccgcgc gcgcgcacgt ggccgcggcc gacctggtga ccgcctacga 12240
gcagacggtg aaccaggagc gcaacttcca aaaaagcttc aacaaccacg tgcgcacgct 12300
ggtggcgcgc gaggaggtga ccctgggtct catgcatctg tgggacctgg tggaggcgat 12360
cgtgcagaac cccagcagca agcccctgac cgcgcagctg ttcctggtgg tgcagcacag 12420
cagggacaac gaggccttca gggaggcgct gctgaacatc accgagccgg aggggcgctg 12480
gctcctggac ctgataaaca tcctgcagag catagtggtg caggagcgca gcctgagcct 12540
ggccgagaag gtggcggcca ttaactattc tatgctgagc ttgggcaagt tctacgcccg 12600
caagatctac aagaccccct acgtgcccat agacaaggag gtgaagatag acagcttcta 12660
catgcgcatg gcgctaaagg tgctgaccct gagcgacgac ctgggagtgt accgcaacga 12720
gcgcatccac aaggccgtga gcgccagccg gcggcgcgag ctgagcgacc gcgagctgat 12780
gcacagtctg cagcgcgcgc tcaccggcgc gggcgagggc gacagggagg tcgagtccta 12840
cttcgacatg ggggctgacc tgcactggca gccgagccgc cgcgccctgg aggcggcggg 12900
ggcgtatggc ggccccctgg cggccgatga cgaggaagag gaggactatg agctagagga 12960
gggcgagtac ctggaggact gacctggctg gtggtgtttt ggtatagatg caagatccga 13020
acgtggcgga cccggcggtc cgggcggcgc tgcagagcca gccgtccggc attaactcct 13080
ctgacgactg ggccgcggcc atgggtcgca tcatggccct gaccgcgcgc aaccccgagg 13140
ccttcaggca gcagcctcag gctaaccggc tggcggccat cttggaagcg gtagtgcccg 13200
cgcgctccaa ccccacccac gagaaggtgc tggccatagt caacgcgctg gcggagagca 13260
gggccatccg ggcggacgag gccggactgg tgtacgatgc gctgctgcag cgggtggcgc 13320
ggtacaacag cggcaacgtg cagaccaacc tggaccgcct ggtgacggac gtgcgcgagg 13380
ccgtggcgca gcgcgagcgc ttgcatcagg acggtaacct gggctcgctg gtggcgctaa 13440
acgccttcct cagcacccag ccggccaacg taccgcgggg gcaggaggac tacaccaact 13500
tcttgagcgc gctgcggctg atggtgaccg aggtccctca gagcgaggtg taccagtcgg 13560
ggcccgacta cttcttccag accagcagac agggcttgca aaccgtgaac ctgagccagg 13620
ctttcaagaa cctgcggggg ctgtggggag tgaaagcgcc caccggcgac cgagctacgg 13680
tgtccagcct gctaaccccc aactcgcgcc tgctgctgct gctgatcgcg cccttcacgg 13740
acagcgggag cgtctcgcgg gagacctatc tgggccacct gctgacgctg taccgcgagg 13800
ccatcgggca ggcgcaggtg gacgagcaca ccttccaaga gatcaccagc gtgagccacg 13860
cgctggggca ggaggacacg ggcagcctgc aggcgaccct gaactacctg ctgaccaaca 13920
ggcggcagaa gattcccacg ctgcacagcc tgacccagga ggaggagcgc atcttgcgct 13980
acgtgcagca gagcgtgagc ctgaacctga tgcgcgacgg cgtgacgccc agcgtggcgc 14040
tggacatgac cgcgcgcaac atggaaccgg gcatgtacgc ttcccagcgg ccgttcatca 14100
accgcctgat ggactacttg catcgggcgg cggccgtgaa ccccgagtac ttcaccaatg 14160
ccattctgaa tccccactgg atgccccctc cgggtttcta caacggagac ttcgaggtgc 14220
ctgaggtcaa tgatgggttc ctctgggatg acatggatga cagtgtgttc tcccccaacc 14280
cgctgcgcgc cgcgtctctg cgattgaagg agggctctga cagggaagga ccgaggagtc 14340
tggcctcctc cctggctctg ggggcggtgg gcgccacggg cgcggcggcg cggggcagca 14400
gccccttccc cagcctggca gactctctga atagcgggcg ggtgagcagg ccccgcttgc 14460
taggcgagga ggagtatctg aacaactccc tgctgcagcc cgtgagggac aaaaacgctc 14520
agcgacagca gtttcccaac aacgggatag agagcctggt ggacaagatg tccagatgga 14580
agacatatgc gcaggagtac aaggagtggg aggaccgcca gccgcggccc ctgccgcccc 14640
ctagacagcg ctggcagcgg cgcgcgtcca accgccgctg gagacaggga cccgaggacg 14700
atgatgactc tgcagatgac agcagcgtgt tggacctggg cgggagcggg aacccctttt 14760
cgcacctgcg cccacgcctg ggcaagatgt tttaaaagag aaaaataaaa ctcaccaagg 14820
ccatggcgac gagcgttggt tttttgttcc cttccttagt atgcggcgcg cggcgatgtt 14880
cgaggagggg cctcccccct cttacgagag cgcgatggga atttctcctg cggcgcccct 14940
gcagcctccc tacgtgcctc ctcggtacct gcaacctaca ggggggagaa atagcatctg 15000
ttactctgag ctgcagcccc tgtacgatac caccagactg tacctggtgg acaacaagtc 15060
cgcggacgtg gcctccctga actaccagaa cgaccacagc gattttttga ccacggtgat 15120
ccaaaacaac gacttcaccc caaccgaggc cagtacccag accataaacc tggacaacag 15180
gtcgaactgg ggcggcgacc tgaagaccat cctgcacacc aacatgccca acgtgaacga 15240
gttcatgttt accaactctt ttaaggcgcg ggttatggtg gcgcgcgagc agggggaggc 15300
gaagtacgag tgggtggact tcacgctgcc cgagggcaac tactcagaga ccatgactat 15360
tgacctgatg aacaatgcga tcgtggaaca ctacctgaaa gtgggcaggc agaacggggt 15420
gaaggagagc gatatcgggg tcaagtttga caccagaaac tttcgtctgg gctgggaccc 15480
cgtgaccggg ctggtcatgc cgggggtcta caccaacgag gcctttcatc ccgatatagt 15540
gctcctgccc ggctgtgggg tggactttac ccagagccgg ctgagcaacc tgctgggcgt 15600
tcgcaagcgg caacctttcc aggagggttt caagatcacc tatgaggatc tggagggggg 15660
caacattccc gcgctccttg atctggacgc ctacgaggag agcttgaaac ccgaggagag 15720
cgctggcgac agcggcgaga gtggcgagga gcaagccggc ggcggtggca gcgcgtcggt 15780
agaaaacgaa agtactcccg cagtggcggc agacgctgcg gaggtcgagc cagaggccat 15840
gcagcaggac gcagaggagg gcgcacagga gggcgcgcag gaggacatga acgatgggga 15900
gatcagggga gacactttcg ccacccgggg cgaagaaaaa gaggcagagg cggtggcggc 15960
gacggtggaa gccgaaaccg aggcagaggc agagcccaag accgaagtta tggaagacat 16020
gaatgatgga gaacgtaggg gtgacacgtt tgccacccgg ggcgaagaga aggcggcgga 16080
ggcagaagcc gcggctgagg aggcggctgc ggctgcggcc gaggctgagg ctgcggctga 16140
ggctaaggtc gaagccgatg ttgcggttga ggctcaggct gaggaggagg aggcggcgac 16200
tgaagcagtt aaggaaaagg cccaggcaga gcaggaagag aaaaaacctg tcattcaacc 16260
tctaaaagaa gatagcaaaa agcgcagtta caacgtcatc gagggcagca cctttaccca 16320
gtaccgcagc tggtacctgg cgtacaacta cggcgacccg gtcaaggggg tgcgctcgtg 16380
gaccctgctc tgcacgccgg acgtcacctg cggctccgag cagatgtatt ggtcgctgcc 16440
aaacatgatg caagacccgg tgaccttccg ctccacgcgg caggttagca acttcccggt 16500
ggtgggcgcc gaactgctgc ccgtgcactc caagagtttt tacaacgagc aggccgtcta 16560
ctcccagctg atccgccagg ccacctctct gacccacgtg ttcaatcgct ttcccgagaa 16620
ccagattttg gcgcgcccgc cggcccccac catcaccacc gtcagtgaaa acgttcctgc 16680
cctcacagat cacgggacgc taccgctgcg caacagcatc tcaggagtcc agcgagtgac 16740
cattactgac gccagacgcc ggacctgccc ctacgtttac aaggccttgg gcatagtctc 16800
gccgcgcgtc ctctccagtc gcacttttta aaatacatct accctcacgc ttcaaaatca 16860
tgtccgtact catctcaccc agcaacaaca ccggctgggg gctgcgcgcg cccagcaaga 16920
tgtttggagg ggcgaggaaa cgctccgagc agcacccagt gcgcgtgcgc ggccactacc 16980
gcgcgccctg gggagcgcac aagcgcgggc gcgcagggcg caccactgtg gacgacgcca 17040
ttgactccgt agtggagcag gcgcgccact acacacccgg cgcgccgtcc gcccccgccg 17100
tgtccaccgt ggacgaggcg atcgagagcg tggtacaggg cgcgcggcac tatgccaacc 17160
ttaaaaatcg acgccgtcgc gtggctcgcc gccatcgccg gagaccccgg gccaccgccg 17220
ccgcgcgcct tgctaaggct ctgctcaggc gcgccaggcg aactggccgc cgggccgcca 17280
tgagggccgc acggcgggct gccgccagcg cggccgccgc ggccccacgg gcacgaaggc 17340
gcgcggccgc tgccgccgcc gccgccattt ccagcttggc ctcgacgcgg cgcggtaaca 17400
tatactgggt gcgcgactcg gtaaccggca cgcgggtacc cgtgcgcttt cgccccccgc 17460
ggaattagca caagacaaca tacacactga gtctcctgct gttgtgtatc ccagcggcga 17520
ccgtcagcag cggcgacatg tccaagcgca aaattaaaga agagatgctc caggtcatcg 17580
cgccggagat ctatgggccc ccgaagaagg aggaggatga ttacaagccc cgcaagctaa 17640
agcgggtcaa aaagaaaaag aaagatgatg acgttgacga ggcggtggag tttgtccgcc 17700
gcatggcgcc caggcgcccc gtgcagtgga agggtcggcg cgtgcagcga gtcctgcgcc 17760
ccggcaccgc ggtggtcttt acgcccggcg agcgttccac gcgcactttc aagcgggtgt 17820
acgatgaggt gtacggcgac gaggatctgt tggagcaggc caaccatcgc tttggggagt 17880
ttgcatatgg gaaacggccc cgcgagagcc taaaagagga cctgctggcg ctaccgctgg 17940
acgagggcaa tcccaccccg agtctgaagc cggtaaccct gcaacaggtg ctgcctttga 18000
gcgcgcccag cgagcagaag cgagggttga agcgcgaggg cggggacctg gcacccaccg 18060
tgcagttgat ggtgcccaag cggcagaagc tggaggacgt gctggagaaa atgaaagtag 18120
agcccgggat ccagcccgaa atcaaggtcc gccccatcaa gcaggtggcg cccggcgtgg 18180
gagtccagac cgtggacgtt aggattccca cggaggagat ggaaacccaa accgccactc 18240
cctcttcggc ggctagcgcc accaccggct ccgcttcggt agaggtgcag acggacccct 18300
ggctagccgc cgccgccccg gccgcccccc gttcgcgcgg gcgcaagaga aattatccag 18360
cggccagcgc gctcatgccc cagtacgcac tgcatccatc catcgcgccc acccccggct 18420
accgcgggta ctcgtaccgc ccgcgcagat cagccggcac ccgcggccgc cgccgccgtg 18480
cgaccacaac cagccgccgc cgtcgccgcc gccgccagcc agtgctgacc cccgtgtctg 18540
taaggaaggt ggctcgctcg gggagcacgc tggtggtgcc cagagcgcgc taccacccca 18600
gcattgttta aagccggtct ctgtatggtt cttgcagata tggccctcac ttgtcgcctc 18660
cgcttcccgg tgccgggata ccgaggaaga actcaccgcc gcagaggcat ggcgggcagt 18720
ggtctccgcg gcggccgtcg ccatcgccgg cgcgcaaaga gcaggcgcat gcgcggcggt 18780
gtgctgccct tcctaatccc gctaatcgcc gcggcgatcg gtgccgtgcc cgggatcgcc 18840
tccgtggccc tgcaggcgtc ccagaaacat tgactcttgc aaccttgcaa gcttgcattt 18900
tttggaggaa aaaataaaaa gtctagactc tcacgctcgc ttggtcctgt gactattttg 18960
tagaaaaaag atggaagaca tcaactttgc gtcgctggcc ccgcgtcacg gctcgcgccc 19020
gttcatggga gactggacag atatcggcac cagcaatatg agcggtggcg ccttcagctg 19080
gggcagtctg tggagtggcc ttaaaaattt tggttccacc attaagaact atggcaacaa 19140
agcgtggaac agcagcacgg gtcagatgct gagagacaag ttgaaagagc agaacttcca 19200
ggagaaggtg gcacagggcc tggcctctgg catcagcggg gtggtggaca tagctaacca 19260
ggccgtgcag aaaaagataa acagtcatct ggacccccgc cctcaggtgg aggaaacgcc 19320
tccagccatg gagacggtgt ctcccgaggg caaaggcgaa aagcgcccgc ggcccgacag 19380
ggaagagacc ctggtgtcac acaccgagga gccgccctct tacgaggagg cagtcaaggc 19440
cggcctgccc accactcgcc ccatagctcc catggccacc ggtgtggtgg gtcacaggca 19500
acacaccccc gcgacactag atctgccccc gccgtccgag ccgactcgcc agccaaaggc 19560
ggtgacggtg cccgctccct ccacttccgc cgccaacaga gtgcctctgc gccgcgctgc 19620
gagcggcccc cgggcctcgc gagtcagcgg caactggcag agcacactga acagcatcgt 19680
gggcctggga gtgaggagtg tgaagcgccg ccgttgctac tgaatgagca agctagctaa 19740
cgtgttgtat gtgtgtatgc gtcctatgtc gccgccagag gagctgttga gccgccggcg 19800
ccgtctgcac tccagcgaat ttcaagatgg cgaccccatc gatgatgcct cagtggtcgt 19860
acatgcacat ctcgggccag gacgcttcgg agtacctgag ccccgggctg gtgcagttcg 19920
cccgcgccac agacacctac ttcaacatga gtaacaagtt caggaacccc actgtggcgc 19980
ccacccacga tgtgaccacg gaccggtcgc agcgcctgac gctgcggttc atccccgtgg 20040
atcgggagga caccgcttac tcttacaagg cgcggttcac gctggccgtg ggcgacaacc 20100
gcgtgctgga catggcctcc acttactttg acatccgggg ggtgctggac aggggcccca 20160
ctttcaagcc ctactcgggc actgcctaca actccctggc ccccaagggc gctcccaatt 20220
cttgcgagtg ggaggaggaa acacaaaatg aggtacaagc caatgaagaa caactagcag 20280
aagaagagga tgaagaaatg gctcaagagg atcagcagcc tactaaaaaa acccatgtat 20340
atgctcaggc acctctttct ggcgaacaga ttaccaaaga tggcttgcaa ataggagctg 20400
aagttacagg agaaacatca aagcccattt ttgcagacaa gacattccaa ccagaacctc 20460
agataggaga gtctcaatgg aatgaggccg atgctacagt agcaggaggt agggttttga 20520
aaaagactac ccctatgaaa ccttgctatg gatcctatgc cagacctacc aatgccaatg 20580
gagggcaggg gatacttgag gcaaatgcta aaggggaact cgaatctaaa gttgagatgc 20640
agtttttctc taacaccaca actcttaatg taagagacgg tgaaaatggc cttaaaccaa 20700
aagtagtgct gtatagcgaa gatgtcaacc tggaatcccc tgacactcat ctgtcttaca 20760
agcccaaaaa agatgatgtt aatgccaaaa tcatgttggg tcagcaagcc atgcccaaca 20820
gacccaacct cattggattt agagataatt tcattgggct catgtattac aacagcactg 20880
gaaacatggg agtgctggcg ggtcaggcct ctcagttgaa tgctgtggtg gacttgcagg 20940
atagaaacac ggaactgtca tatcagctta tgcttgattc cattggagat agaaccagat 21000
acttttccat gtggaaccag gcagtggata gctatgaccc agatgttaga atcattgaaa 21060
accatggggt ggaggatgag ctgcccaact actgttttcc cttgggcggt ataggaatta 21120
cagatacata ccaggccata aaagcagcca atggtggaga tgctactacg tggtctgctg 21180
ataacacatt tgcagaccgc aacgaaatag gggtgggaaa caacttcgcc atggagatca 21240
acatccaggc caacctctgg agaaacttcc tctatgcgaa cgtgggactc tacctgccag 21300
acaagctcaa gtacaacccc accaacgtgg acatctctga caaccccaac acctatgact 21360
acatgaacaa gcgggtggtg gcccccggcc tggtggactg ctttgtcaat gtgggagcca 21420
ggtggtccct ggactacatg gacaacgtca accccttcaa ccaccaccgc aatgcgggtc 21480
tgcgctaccg ctccatgatc ctgggcaacg ggcgctatgt gcccttccac atccaggtac 21540
cccagaagtt ctttgccatc aagaacctcc tgctcctgcc cggctcctac acctacgagt 21600
ggaacttcag gaaggatgta aacatggtcc tacagagctc tctgggcaat gaccttaggg 21660
tagatggggc cagcatcaag tttgacagca tcaccctcta tgctacattt ttccccatgg 21720
cccacaacac cgcctccacg cttgaggcca tgctgagaaa cgacaccaac gaccagtcct 21780
tcaatgacta cctctctggg gccaacatgc tctacccaat cccagccaag gccaccaacg 21840
tgcccatctc catcccctct cgcaactggg ccgcctttag aggctgggcc tttacccgcc 21900
ttaagaccaa ggagaccccc tccctgggct cgggttttga tccctacttt gtttactcgg 21960
gatccatccc ctacctggat ggcaccttct acctcaacca cactttcaag aagatatcca 22020
tcatgtatga ctcctccgtc agctggccgg gcaacgaccg cttgctcacc cccaatgagt 22080
tcgaggtcaa gcgcgccgtg gacggcgagg gctacaacgt ggcccagtgc aacatgacca 22140
aggactggtt cctggtgcag atgctggcca actacaacat aggctaccag ggcttctaca 22200
tcccagagag ctacaaggac aggatgtatt ccttcttcag aaatttccaa cccatgagcc 22260
gacaggtggt ggacgagacc aattacaagg actatcaggc cattggcatc acccaccagc 22320
acaacaactc gggtttcgtg ggctacctgg cgcccaccat gcgcgaggga caggcctacc 22380
ccgccaactt cccctacccc ctgataggca agaccgcggt cgacagcgtc acccagaaaa 22440
agttcctctg cgaccgcacc ctctggcgca tccccttctc tagcaacttt atgtccatgg 22500
gtgcgctcac ggacctgggc caaaacctgc tttatgccaa ctctgcccat gcgctggaca 22560
tgacttttga ggtggacccc atggacgagc ccacccttct ctatattgtg tttgaagtgt 22620
tcgacgtggt cagagtgcac cagccgcacc gcggtgtcat cgagaccgtg tacctgcgta 22680
cgcccttctc agccggcaac gccaccacct aaggagacag cgccgccgcc tgcatgactg 22740
gttccaccga gcaagagctc agggccatcg ccagagacct gggatgcgga ccctattttt 22800
tgggcaccta tgacaaacgc ttcccgggtt ttatctcccg agacaagctc gcctgcgcca 22860
tcgtcaacac ggccgcgcgc gagaccgggg gcgtgcactg gctggccttt ggctgggacc 22920
cgcgctctaa aacttgctac ctctttgacc cctttggctt ctccgatcag cgcctcaggc 22980
agatttatga gtttgagtac gaggggctgt tgcgccgcag cgcgcttgcc tcctcgcccg 23040
accgctgcat cacccttgag aagtccaccg agaccgtgca ggggccccac tcggccgcct 23100
gcggtctctt ctgttgcatg tttttgcacg cctttgtgca ctggcctcag agtcccatgg 23160
atcgcaaccc caccatgaac ttgctaaagg gagtgcccaa cgccatgctc cagagccccc 23220
aggtcctgcc caccctgcgc cgcaaccagg aacagctcta ccgcttcctg gagcgccact 23280
ccccctactt ccgcagccac agcgcgcgca tccggggggc cacctctttt tgccacttgc 23340
aagaaaacat gcaagacgga aaatgatgta cagcatgctt ttaataaatg taaagactgt 23400
gcactttatt tatacacggg ctctttctgg ttatttattc aacaccgccg tcgccatcta 23460
gaaatcgaaa gggttctgcc gcgcgtcgcc gtgcgccacg ggcagagaca cgttgcgata 23520
ctggaagcgg ctcgcccact tgaactcggg caccaccatg cggggcagtg gttcctcggg 23580
gaaattctcg ctccacaggg tgcgggtcag ctgcagcgcg ctcaggaggt cgggagccga 23640
gatcttgaag tcgcagttgg ggccggaacc ctgcgcgcgc gagttgcggt acacggggtt 23700
gcagcactgg aacaccagca gggccggatt attcacgctg gccagcaggc tctcgtcgct 23760
aatcatgtcg ctgtccagat cctccgcgtt gctcagggcg aatggggtca tcttgcagac 23820
ctgcctgccc aggaaaggcg ggagcccagg cttgccgtta cagtcgcagc gcaggggcat 23880
tagcaggtgc ccacggcccg actgcgcctg cgggtacaac gcgcgcatga aggcttcgat 23940
ctgcctaaaa gccacctggg tcttggctcc ctccgaaaag aacatcccac aggacttgct 24000
ggagaactgg ttcgcgggac agctggcatc gtgcaggcag cagcgcgcgt cagtgttggc 24060
gatctgcacc acgttgcgac cccaccggtt tttcactatc ttggccttgg aagcctgctc 24120
ctttagcgcg cgctggccgt tctcgctggt cacatccatc tctatcacct gttccttgtt 24180
gatcatgttt gtcccgtgca gacactttag gtcgccctcc gtctgggtgc agcggtgctc 24240
ccacagcgcg caaccggtgg gctcccaatt tttgtgggtc acccccgcgt aggcctgcag 24300
gtaggcctgc aggaagcgcc ccatcatggt cataaaggtc ttctggctcg taaaggtcag 24360
ctgcaggccg cgatgctctt cgttcagcca ggtcttgcag atggcggcca gcgcctcggt 24420
ctgctcgggc agcatcttaa aatttgtctt caggtcgtta tccacgtggt acttgtccat 24480
catggcacgc gccgcctcca tgcccttctc ccaggcggac accatgggca ggcttagggg 24540
gtttatcact tccagcggcg aggacaccgt actttcgatt tcttcttcct ccccctcttc 24600
ccggcgcgcg cccccgctgt tgcgcgctct taccgcctgc accaaggggt cgtcttcagg 24660
caagcgccgc accgagcgct tgccgccctt gacctgcttg atcagtaccg gcgggttgct 24720
gaagcccacc atggtcagcg ccgcctgctc ttcttcgtct tcgctgtcta ccactatttc 24780
tggggagggg cttctccgct ctgcggcaaa ggcggcggat cgcttctttt ttttcttggg 24840
agccgccgcg atggagtccg ccacggcgac cgaggtcgag ggcgtggggc tgggggtgcg 24900
cggcaccagg gcctcgtcgc cctcggactc ttcctctgac tccaggcggc ggcggagtcg 24960
cttctttggg ggcgcgcgcg ttagcggcgg cggagacggg gacggggacg gggacgggac 25020
gccctccaca gggggcggtc ttcgcgcaga cccgcggccg cgctcggggg tcttctcgcg 25080
ctggtcttgg tcccgactgg ccattgtatc ctcctcctcc taggcagaga gacataagga 25140
gtctatcatg caagtcgaga aggaggagag cttaaccacc ccctctgaga ccgccgtcgc 25200
cgtcgccccc gctaccgccg acgcgcccgc cacaccgagc gacacccccg cggacccccc 25260
cgccgacgca cccctgttcg aggaagcggc cgtggagcag gacccgggct ttgtctcggc 25320
agaggaggat ttgcaagagg aggaggataa ggaggagaag ccctcagtgc caaaagatca 25380
taaagagcaa gacgagcacg acgcagacgc acaccagggt gaagtcgggc ggggggacgg 25440
agggcatggc ggcgccgact acctagacga aggaaacgac gtgctcttga agcacctgca 25500
tcgtcagtgc gccattgtct gcgacgctct gcaggagcgc agcgaggtgc ccctcagcgt 25560
ggcggaggtc agccgcgcct acgagctcag cctcttttcc ccccgggtgc ccccccgccg 25620
ccgcgaaaac ggcacatgcg agcccaaccc gcgcctcaac ttctaccccg cctttgtggt 25680
gcccgaggtc ctggccacct atcacatctt ctttcaaaat tgcaagatcc ccatctcgtg 25740
ccgcgccaac cgtagccgcg ccgataagat gctggccctg cgccagggcg accacatacc 25800
tgatatcgcc gctttggaag atgtgccaaa gatcttcgag ggtctgggtc gcaacgagaa 25860
gcgggcagca aactctctgc aacaggaaaa cagcgaaaat gagagtcaca ctggagcgct 25920
ggtggagctg gagggcgaca acgcccgcct ggcggtgctc aagcgcagca tcgaggtcac 25980
ccactttgcc taccccgcgc tcaacctgcc ccccaaagtc atgaacgcgg tcatggacgg 26040
gctaatcatg cgccgcggcc ggccccttgc tccagatgca aacttgcatg aggagaccga 26100
ggacggtcag cccgtggtca gcgacgagca gctgacgcgc tggctggaaa ccgcggaccc 26160
cgccgaactg gaggagcggc gcaagatgat gatggccgcg gtgctggtca ccgtagagct 26220
ggagtgtctg cagcgcttct tcggcgaccc cgagatgcag agaaaggtcg aggagaccct 26280
acactacacc ttccgccagg gctacgtgcg ccaggcttgc aagatctcca acgtggagct 26340
cagcaacctg gtgtcctacc tgggcatctt gcatgaaaac cgccttgggc agagcgtgct 26400
acactccacc ctgcgcgggg aggcgcgccg cgactacgtg cgcgactgcg tttacctctt 26460
cctctgctac acctggcaga cggccatggg ggtctggcag cagtgcctgg aggagcgcaa 26520
cctcaaggag ctggagaagc tcctgcagcg cgcgctcaaa gacctctgga cgggctacaa 26580
cgagcgctcg gtggccgccg cgctggccga cctcatcttc cccgagcgcc tgctcaaaac 26640
tctccagcag gggctgcccg acttcaccag ccaaagcatg ttgcaaaatt ttaggaactt 26700
tatcctggag cgttctggca tcctacccgc cacctgctgc gccctgccca gcgactttgt 26760
ccccctcgtg taccgcgagt gccccccgcc gctgtggggc cactgctacc tgttccaact 26820
ggccaactac ctgtcctacc acgcggacct catggaagac tccagcggcg aggggctcat 26880
ggagtgccac tgccgctgca acctctgcac gccccaccgc tccctggtct gcaacaccca 26940
actgctcagc gagagtcaga ttatcggtac cttcgagcta cagggtccgt cctcctcaga 27000
cgagaagtcc gcggctccgg ggctaaaact cactccgggg ctgtggactt ccgcctacct 27060
gcgcaaattt gtacctgaag actaccacgc ccacgagatc aggttttacg aggaccaatc 27120
ccgcccgccc aaggcggagc tgaccgcctg cgtcatcacc cagggcgaga tcctaggcca 27180
attgcaagcc atccaaaaag cccgccaaga gtttttgctg agaaagggtc ggggggtgta 27240
tctggacccc cagtcgggtg aggagctcaa cccggttccc ccgctgccgc cgccgcggga 27300
ccttgcttcc caggataagc atcgccatgg ctcccagaaa gaagcagcag cggccgccac 27360
tgccgccacc ccacacgctg gaggaagagg aggaatactg ggacagtcag gcagaggagg 27420
tttcggacga ggaggagccg gagacggaga tggaagagtg ggaggaggac agcttagacg 27480
aggaggcttc cgaagccgaa gaggcaggcg caacaccgtc accctcggcc gcagccccct 27540
cgcaggcgcc cccgaagtcc gctcccagca tcagcagcaa cagcagcgct ataacctccg 27600
ctcctccacc gccgcgaccc acggccgacc gcagacccaa ccgtagatgg gacaccaccg 27660
gaaccggggc cggtaagtcc tccgggaaag gcaagcaagc gcagcgccaa ggctaccgct 27720
cgtggcgcgc tcacaagaac gccatagtcg cttgcttgca agactgcggg gggaacatct 27780
ccttcgcccg ccgcttcctg ctcttccacc acggtgtggc cttcccccgt aacgtcctgc 27840
attactaccg tcatctctac agcccctact gcggcggcag tgagccagag gcggccggcg 27900
gcagcggcgc ccgtttcggt gcctaggaag acccagggca agacttcagc caagaaactc 27960
gcggcggccg cggcgaacgc ggtcgcgggg gccctgcgcc tgacggtgaa cgaacccctg 28020
tcgacccgcg aactgaggaa ccgaatcttc cccactctct atgccatctt ccagcagagc 28080
agagggcagg atcaggaact gaaagtaaaa aacaggtctc tgcgctccct cacccgcagc 28140
tgtctgtatc acaagagcga agaccagctt cggcgcacgc tggaggacgc tgaggcactc 28200
ttcagcaaat actgcgcgct cactcttaag gactagctcc gcgcccttct cgaatttagg 28260
cgggaacgcc tacgtcatcg cagcgccgcc gtcatgagca aggacattcc cacgccatac 28320
atgtggagct atcagccgca gatgggactc gcggcgggcg cctcccagga ttactccacc 28380
cgcatgaact ggctcagtgc cggcccacac atgatctcac aggttaatga catccgcacc 28440
catcgaaacc aaatattggt ggagcaggcg gcaattacca ccacgccccg caataatccc 28500
aaccccaggg agtggcccgc gtccctggtg tatcaggaaa ttcccggccc caccaccgta 28560
ctacttccgc gtgattccca ggccgaagtc caaatgacta actcaggggc acagctcgcg 28620
ggcggctgtc gtcacagggt gcggcctcct cgccagggta taactcacct gaagatccga 28680
ggcagaggta ttcagctcaa cgacgagtcg gtgagctcct cgctcggtct cagacctgac 28740
gggaccttcc agatagccgg agccggccga tcttccttca cgccccgcca ggcgtacctg 28800
actctgcaga gctcgtcctc ggcgccgcgc tcgggcggca tcgggactct ccagttcgtg 28860
caggagtttg tgccctcggt ctacttcaac cccttctcgg gctctcccgg tcgctacccg 28920
gaccagttca tcccgaactt tgacgccgcg agggactcgg tggacggcta cgactgaatg 28980
tcgggtggac ccggtgcaga gcaacttcgc ctgaagcacc ttgaccactg ccgccgccct 29040
cagtgctttg cccgctgtca gaccggtgag ttccagtact tttccctgcc cgactcgcac 29100
ccggacggcc cggcgcacgg ggtgcgcttt ttcatcccga gtcaggtccg ctctacccta 29160
atcagggagt tcaccgcccg tcccctactg gcggagttgg aaaaggggcc ttctatccta 29220
accattgcct gcatctgctc taaccctgga ttacaccaag atctttgctg tcatttgtgt 29280
gctgagtata ataaaggctg agatcagaat ctactcgggc tcctgtcgcc atcctgtcaa 29340
cgccaccgtc caagcccggc ccgatcagcc cgaggtgaac ctcacctgcg gtctgcaccg 29400
gcgcctgagg aaatacctag cttggtacta caacagcact ccctttgtgg tttacaacag 29460
ctttgaccag gacggggtct cactgaggga taacctctcg aacctgagct actccatcag 29520
gaagaacaac accctcgagc tacttcctcc ttacctgccc gggacttacc agtgtgtcac 29580
cggtccctgc acccacaccc acctgttgat cgtaaacgac tctcttccga gaacagacct 29640
caataactcc tctccgcagt tccccagaac aggaggtgag ctcaggaaac cccgggtaaa 29700
gaagggtgga caagagttaa cacttgtggg gtttctggtg tatgtgacgc tggtggtggc 29760
tcttttgatt aaggcttttc cttccatgtc tgaactatcc ctcttctttt atgaacaact 29820
cgactagtgc taacgggacc ctacccaacg aatcgggatt gaatatcggt aaccaggttg 29880
cagtttcact tttgattacc ttcatagtcc tcttcctgct agtgctgtcg cttctgtgcc 29940
tgcggatcgg gggctgctgc atccacgttt atatctggtg ctggctgttt agaaggttcg 30000
gagaccaccg caggtagaat aaacaaacct agacctagaa atggacggtc tctgcagcga 30060
gcaacgcata ctagagaggc gccggcaaaa agcagagctc gagcgtctta aacaagagct 30120
ccaagacgcc gtggccatac accagtgcaa aaaagggctc ttctgtctgg taaaacaggc 30180
cacgctcacc tatgaaaaaa caggtgacac ccaccgccta ggatacaagc tgcccacaca 30240
gcgccaaaag tttgccctta tgataggtga acaacccatc accgtgaccc agcactccgt 30300
ggagacagaa ggctgcattc atgctccctg caggggcgct gactgcctct acaccttgat 30360
caaaaccctt tgcggtctca gagaccttat ccctttcaat tgatcataac tgtaatcaat 30420
aaaaaatcac ttacttgaaa tctgatagca agcctctgtc caattttttc agcaacactt 30480
ccttcccctc ttcccaactc tggtactcta ggcgcctcct agctgcaaac ttcctccaca 30540
gtctgaaggg aatgtcagat tcctcctcct cctgtccctc cgcacccacg atcttcatgt 30600
tgttgcagat gaagcgcacc aaaacgtctg acgagagctt caaccccgtg tacccctatg 30660
acacggaaaa cggtcctccc tccgtccctt tcctcacccc tcccttcgtg tctcccgatg 30720
gattccaaga gagccccccc ggggtcctgt ctctgaacct ggccgagccc ctggtcactt 30780
cccacggcat gctcgccctg aaaatgggaa gtggcctctc cctggacgac gccggcaacc 30840
tcacctctca agatgtcacc accactaccc ctcccctgaa aaaaaccaag accaacctca 30900
gcctagaaac ctcagccccc ctgactgtga gcacctcagg cgccctcacc ctagcagccg 30960
ccgttcccct ggcggtggcc ggcacctccc tcaccatgca atcagaggcc cccctgacag 31020
tccaagatgc aaaactcacc ctggccacca agggccccct gaccgtgtct gaaggcaaac 31080
tggccttgca gacctcggcc ccgctgacgg ccgctgacag cagcgccctc accgttagcg 31140
ccacaccacc catcagtgta agcagtggaa gtttgggctt agacatggaa gaccccatgt 31200
atactcatga tggaaaactg ggaataagaa ttggaggccc actgagagta gtagacagcc 31260
tgcacacact gactgtagtt accggaaatg gaatagctgt agataacaat gccctccaaa 31320
ctagagttac gggcgccctg ggttatgaca catcaggaaa cctacaactg agagccgcgg 31380
ggggtatgcg aattgatgca aatggccaac ttatccttga tgtggcatac ccatttgatg 31440
ctcaaaacaa tctcagcctt agacttggtc agggacccct gtatgtaaac acagaccaca 31500
acctagattt gaattgcaac agaggtctga ccacaactac caccaacaac acaaaaaaac 31560
ttgaaactaa aattggctca ggcttagact atgataccaa tggtgctgtc attattaaac 31620
ttggtactgg tgtaagcttt gacagcacag gcgccctaac tgtgggaaac actggcgatg 31680
ataaactgac tctgtggaca accccagacc catctccaaa ttgcagaatt cacgcagaca 31740
aagactgcaa gtttactcta gtcctaacta agtgtggaag tcaaatcctg gcttctgtcg 31800
ccgccctagc ggtgtcagga aatctggctt caataacagg caccgttgcc agcgttacca 31860
tctttctcag atttgatcag aatggagtgc ttatggaaaa ctcctcccta gacaagcagt 31920
actggaactt cagaaatggt aactcaacca atgccacccc ctacaccaat gcagttgggt 31980
tcatgccaaa cctcgcagca taccccaaga cacagagcca gactgctaaa aacaacattg 32040
taagtcaggt ttacttgaat ggggacaaat ccaaacccat gacccttacc attaccctca 32100
atggaactaa tgaatccagt gaaactagcc aggtgagtca ctactccatg tcatttacat 32160
gggcttggga gagtgggcaa tatgccaccg aaacctttgc caccaattcc tttaccttct 32220
cttacattgc tgaacaataa agaaagcaca gagatgcttg tttttgattt caaaattgtg 32280
tgcttttatt tattttcaag cttacagtat ttccagtagt cattcgaata gagcttaatg 32340
aaactgcatg agaacccttc cacatagctt aaattatcac cagtgcaaat ggagaaaaat 32400
caacatacct ttttatccag atatcacaga accctagtat tcaacctgcc acctccctcc 32460
caacacacag agtacacagt cctttctccc cggctggcct taaaaagcat catatcatgg 32520
gtaacagaca tattcttagg tgttatattc cacacggttt cctgtcgagc caaacgctca 32580
tcagtgatat taataaactc cccgggcagc tcacttaagt tcatgtcgct gtccagctgc 32640
tgagccacag gctgctgtcc aacttgcggt tgcttaacgg gcggcgaagg agaagtccac 32700
gcctacatgg gggtagagtc ataatcgtgc atcaggatag ggcggtggtg ctgcagcagc 32760
gcgcgaataa actgctgccg ccgccgctcc gtcctgcagg aatacaacat ggcagtggtc 32820
tcctcagcga tgattcgcac cgcccgcagc ataaggcgcc ttgtcctccg ggcacagcag 32880
cgcaccctga tctcacttaa atcagcacag taactgcagc acagcaccac aatattgttc 32940
aaaatcccac agtgcaaggc gctgtatcca aagctcatgg cggggaccac agaacccacg 33000
tggccatcat accacaagcg caggtagatt aagtggcgac ccctcataaa cacgctggac 33060
ataaacatta cctcttttgg catgttgtaa ttcaccacct cccggtacca tataaacctc 33120
tgattaaaca tggcgccatc caccaccatc ctaaaccagc tggccaaaac ctgcccgccg 33180
gctatacact gcagggaacc gggactggaa caatgacagt ggagagccca ggactcgtaa 33240
ccatggatca tcatgctcgt catgatatca atgttggcac aacacaggca cacgtgcata 33300
cacttcctca ggattacaag ctcctcccgc gttagaacca tatcccaggg aacaacccat 33360
tcctgaatca gcgtaaatcc cacactgcag ggaagacctc gcacgtaact cacgttgtgc 33420
attgtcaaag tgttacattc gggcagcagc ggatgatcct ccagtatggt agcgcgggtt 33480
tctgtctcaa aaggaggtag acgatcccta ctgtacggag tgcgccgaga caaccgagat 33540
cgtgttggtc gtagtgtcat gccaaatgga acgccggacg tagtcatatt tcctccagca 33600
gaaccaagtg cgcgcgtggc agctatcctt gcgtcttctg tctcgccgcc tgccccgctc 33660
ggtgtagtag ttgtaataca gccactccct cagaccgtca aggcgctccc tggcgtccgg 33720
atctataaca acaccatcct gcagcgccgc cctgatgaca tccaccaccg tagagtatgc 33780
caagcccagc caggaaatgc actcactttg acagcgagag ataggaggag cgggaagaga 33840
tggaagaacc atgatagtaa aagaactttt attccaatcg atcctctaca atgtcaaagt 33900
gtagatctat cagatggcac tggtctcctc cgctgagtcg atcaaaaata acagctaaac 33960
cacaaacaac acgattggtc aaatgctgca caagggcttg cagcataaaa tcgcctcgaa 34020
agtccaccgc aagcataaca tcaaagccac cgcccctatc atgatctatg ataaaaaccc 34080
cacagctatc caccagaccc atatagtttt catctctcca tcgtgaaaaa atatttacaa 34140
gctcctcctt taaatcacct ccaaccaatt caaaaagttg agccagaccg ccctccacct 34200
tcattttcag catgcgcatc atgattgcaa aaattcaggc tcctcagaca cctgtataag 34260
attgagaagc ggaacattaa catcaatgtt tcgctcgcga agatcgcgcc tcagtgcaag 34320
catgatataa tcccacaggt cggagcggat cagcgaggac atctccccgc caggaaccaa 34380
ctcaacggag cctatgctga ttataatacg catattcggg gctatgctaa ccagcacggc 34440
ccccaaatag gcgtactgca taggcggcga caaaaagtga acagtttggg ttaaaaaatc 34500
aggcaaacac tcgcgcaaaa aagcaagaac atcataacca tgctcatgca aatagatgca 34560
agtaagctca ggaacgacca cagaaaaatg cacaattttt ctctcaaaca tgactgcgag 34620
ccctgcaaaa aataaaaaag aaacattaca caagagtagc ctgtcttaca atgggataga 34680
ctactctaac caacataaga cgggccacga catcgcccgc gtggccataa aaaaaattat 34740
ccgtgtgatt aaaaagaagc acagatagct ggccagtcat atccggagtc atcacgtgcg 34800
aacccgtgta gacccccggg ttggacacat cggccaaaga aagaaagcgg ccaatgtatc 34860
ccggaggaat gataacacta agacgaagat acaacagaat aaccccatgg gggggaataa 34920
caaagttagt aggtgaataa aaacgataaa cacccgaaac tccctcctgc gtaggcaaaa 34980
tagcgccctc cccttccaaa acaacataca gcgcttccac agcagccatg acaaaagact 35040
caaaacactc aaaagactca gtcttaccag gaaaataaaa gcactctcac agcaccagca 35100
ctaatcagag tgtgaagagg gccaagtgcc gaacgagtat atataggaat taaaaatgac 35160
gtaaatgtgt aaaggtcaaa aaacgcccag aaaaatacac agaccaacgc ccgaaacgaa 35220
aacccgcgaa aaaataccca gaagttcctc aacaaccgcc acttccgctt tcccacgata 35280
cgtcacttcc tcgaaaatag caaactacat ttcccacatg tacaaaaccg aaaccactcc 35340
ccttgtcacc gcccacaact tacatcttaa ttaacaaacg tcaaagccta cgtcagccgc 35400
cccgcctcgc cccgcccacc tcattatcat attggccaca atccaaaata aggtatatta 35460
ttgatgatg 35469
<210> 19
<211> 183
<212> PRT
<213> 人工序列
<220>
<223> BZ28纤维突结
<400> 19
Thr Leu Trp Thr Thr Pro Asp Pro Ser Pro Asn Cys Arg Ile His Ala
1 5 10 15
Asp Lys Asp Cys Lys Phe Thr Leu Val Leu Thr Lys Cys Gly Ser Gln
20 25 30
Ile Leu Ala Ser Val Ala Ala Leu Ala Val Ser Gly Asn Leu Ala Ser
35 40 45
Ile Thr Gly Thr Val Ala Ser Val Thr Ile Phe Leu Arg Phe Asp Gln
50 55 60
Asn Gly Val Leu Met Glu Asn Ser Ser Leu Asp Lys Gln Tyr Trp Asn
65 70 75 80
Phe Arg Asn Gly Asn Ser Thr Asn Ala Thr Pro Tyr Thr Asn Ala Val
85 90 95
Gly Phe Met Pro Asn Leu Ala Ala Tyr Pro Lys Thr Gln Ser Gln Thr
100 105 110
Ala Lys Asn Asn Ile Val Ser Gln Val Tyr Leu Asn Gly Asp Lys Ser
115 120 125
Lys Pro Met Thr Leu Thr Ile Thr Leu Asn Gly Thr Asn Glu Ser Ser
130 135 140
Glu Thr Ser Gln Val Ser His Tyr Ser Met Ser Phe Thr Trp Ala Trp
145 150 155 160
Glu Ser Gly Gln Tyr Ala Thr Glu Thr Phe Ala Thr Asn Ser Phe Thr
165 170 175
Phe Ser Tyr Ile Ala Glu Gln
180

Claims (14)

1.一种分离的核酸序列，该分离的核酸序列编码六邻体多肽，该六邻体多肽由SEQ IDNO: 2的氨基酸序列组成。
2.如权利要求1所述的分离的核酸序列，该分离的核酸序列进一步包含编码由SEQ IDNO: 3的氨基酸序列组成的纤维多肽的核酸序列。
3.一种分离的核酸序列，该分离的核酸序列编码纤维多肽，该纤维多肽由SEQ ID NO:3的氨基酸序列组成。
4.一种载体，该载体包含如权利要求1-3中任一项所述的核酸序列。
5.如权利要求4所述的载体，该载体为腺病毒载体，并且进一步包含转基因。
6.如权利要求5所述的载体，其中该腺病毒载体进一步包含E1缺失和E3缺失中的至少一种。
7.如权利要求5或6所述的载体，其中该腺病毒载体是包含一个或多个人腺病毒核酸序列的嵌合腺病毒载体。
8.如权利要求7所述的载体，其中所述人腺病毒核酸序列来自人腺病毒-4、人腺病毒-5、人腺病毒-26或人腺病毒-35中的至少一种。
9.如权利要求5或6所述的载体，其中该腺病毒载体包含选自由SEQ ID NO: 9和SEQ IDNO: 10组成的组的核酸序列。
10.一种重组细胞，该重组细胞包含如权利要求4-9中任一项所述的载体。
11.一种生产载体的方法，该方法包括：a.使如权利要求10所述的重组细胞在用于生产该载体的条件下生长；以及b.从该重组细胞中分离该载体。
12.一种免疫原性组合物，该免疫原性组合物包含如权利要求5-9中任一项所述的载体。
13.如权利要求12所述的免疫原性组合物在制备用于在有需要的受试者中诱导免疫应答的药物中的用途。
14.一种生产疫苗的方法，该方法包括将如权利要求5-9中任一项所述的载体与药学上可接受的载剂组合。
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Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2971008B1 (en) 2013-03-14 2018-07-25 Salk Institute for Biological Studies Oncolytic adenovirus compositions
JP7015551B2 (ja) 2016-02-23 2022-02-15 ソーク インスティテュート フォー バイオロジカル スタディーズ ウイルス動態への影響を最小限にするための治療用アデノウイルスにおける外因性遺伝子発現
JP7054527B2 (ja) 2016-02-23 2022-04-14 ソーク インスティテュート フォー バイオロジカル スタディーズ アデノウイルスの複製動態を測定するための高スループットアッセイ
CA3045892A1 (en) 2016-12-12 2018-06-21 Salk Institute For Biological Studies Tumor-targeting synthetic adenoviruses and uses thereof
US11498944B2 (en) 2020-01-31 2022-11-15 Janssen Pharmaceuticals, Inc. Compositions and methods for preventing and treating coronavirus infection—SARS-CoV-2 vaccines
BR112022022937A2 (pt) 2020-05-11 2022-12-20 Janssen Pharmaceuticals Inc Proteínas de fusão estabilizadas de proteína de pico de coronavírus
MX2023000024A (es) 2020-06-29 2023-04-12 Janssen Vaccines & Prevention Bv Combinación vacunal contra la infección por el virus respiratorio sincicial.
TW202208398A (zh) * 2020-07-01 2022-03-01 義大利商萊伊錫拉有限責任公司 大猩猩腺病毒核酸及胺基酸序列，包含彼之載體，及其用途
CN115916804A (zh) 2020-07-06 2023-04-04 杨森制药公司 稳定的冠状病毒刺突蛋白融合蛋白
JP2024509756A (ja) 2021-02-19 2024-03-05 ヤンセン ファッシンズ アンド プリベンション ベーフェー 安定化された融合前ｒｓｖ ｆｂ抗原
WO2022175479A1 (en) 2021-02-19 2022-08-25 Janssen Vaccines & Prevention B.V. Vaccine combinations against respiratory syncytial virus strain a and b infections
CN116867517A (zh) 2021-02-23 2023-10-10 扬森疫苗与预防公司 三聚体稳定性hiv包膜蛋白突变
CA3214415A1 (en) 2021-04-01 2022-10-06 Janssen Vaccines & Prevention B.V. Stabilized pre-fusion piv3 f proteins
WO2023020939A1 (en) 2021-08-17 2023-02-23 Janssen Pharmaceuticals, Inc. Sars-cov-2 vaccines
WO2023026182A1 (en) 2021-08-24 2023-03-02 Janssen Pharmaceuticals, Inc. Sars-cov-2 vaccines
WO2023047348A1 (en) 2021-09-24 2023-03-30 Janssen Pharmaceuticals, Inc. Stabilized corona virus spike protein fusion proteins
WO2023047349A1 (en) 2021-09-24 2023-03-30 Janssen Pharmaceuticals, Inc. Stabilized coronavirus spike protein fusion proteins
WO2023111725A1 (en) 2021-12-14 2023-06-22 Janssen Pharmaceuticals, Inc. Sars-cov-2 vaccines
WO2023110618A1 (en) 2021-12-16 2023-06-22 Janssen Vaccines & Prevention B.V. Stabilized pre-fusion hmpv fusion proteins
WO2023156505A1 (en) 2022-02-17 2023-08-24 Janssen Vaccines & Prevention B.V. Trimer stabilizing hiv envelope protein mutations r304v, n302m and t320l
WO2024061759A1 (en) 2022-09-23 2024-03-28 Janssen Vaccines & Prevention B.V. Stabilized coronavirus s proteins
WO2024061757A1 (en) 2022-09-23 2024-03-28 Janssen Vaccines & Prevention B.V. Pre-fusion human piv1 f proteins
WO2024074584A1 (en) 2022-10-06 2024-04-11 Janssen Vaccines & Prevention B.V. Stabilized pre-fusion piv3 f proteins

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005071093A2 (en) * 2004-01-23 2005-08-04 Istituto Di Ricerche Di Biologia Molecolare P Angeletti Spa Chimpanzee adenovirus vaccine carriers
CN105112428A (zh) * 2009-02-02 2015-12-02 葛兰素史密斯克莱生物公司 猿腺病毒核酸和氨基酸序列，包含其的载体及其用途

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2705686B1 (fr) 1993-05-28 1995-08-18 Transgene Sa Nouveaux adénovirus défectifs et lignées de complémentation correspondantes.
US5851806A (en) 1994-06-10 1998-12-22 Genvec, Inc. Complementary adenoviral systems and cell lines
EP0784690B1 (en) 1994-06-10 2006-08-16 Genvec, Inc. Complementary adenoviral vector systems and cell lines
US5846782A (en) 1995-11-28 1998-12-08 Genvec, Inc. Targeting adenovirus with use of constrained peptide motifs
US5559099A (en) 1994-09-08 1996-09-24 Genvec, Inc. Penton base protein and methods of using same
US5965541A (en) 1995-11-28 1999-10-12 Genvec, Inc. Vectors and methods for gene transfer to cells
SI0833934T2 (sl) 1995-06-15 2013-04-30 Crucell Holland B.V. Pakirni sistemi za humani rekombinantni adenovirus za uporabo v genski terapiji
US5837511A (en) 1995-10-02 1998-11-17 Cornell Research Foundation, Inc. Non-group C adenoviral vectors
CA2177085C (en) 1996-04-26 2007-08-14 National Research Council Of Canada Adenovirus e1-complementing cell lines
EP0973866A4 (en) 1997-03-04 2000-04-19 Baxter Int ADENOVIRUS E1-COMPLEMENTING CELL LINES
US6020191A (en) 1997-04-14 2000-02-01 Genzyme Corporation Adenoviral vectors capable of facilitating increased persistence of transgene expression
US5981225A (en) 1998-04-16 1999-11-09 Baylor College Of Medicine Gene transfer vector, recombinant adenovirus particles containing the same, method for producing the same and method of use of the same
US6113913A (en) 1998-06-26 2000-09-05 Genvec, Inc. Recombinant adenovirus
EP1200622A4 (en) 1999-07-06 2004-12-22 Merck & Co Inc HIV VACCINE COMPRISING A GAG GENE VEHICLE ADENOVIRUS
DE19955558C2 (de) 1999-11-18 2003-03-20 Stefan Kochanek Permanente Amniozyten-Zelllinie, ihre Herstellung und Verwendung zur Herstellung von Gentransfervektoren
EP1320621A4 (en) 2000-09-15 2005-11-23 Merck & Co Inc ADVANCED FIRST-GENE ADENOVIRAL VACCINES EXPRESSING CODON OPTIMIZATION OF THE GAG, POL AND NEF PROTEINS OF HIV-1 AND CHANGES THEREOF
EP1409012B1 (en) 2001-06-22 2009-02-11 The Wistar Institute Of Anatomy And Biology Methods of inducing a cytotoxic immune response and recombinant simian adenovirus compositions useful therein
PL215165B1 (pl) 2002-04-25 2013-10-31 Crucell Holland Bv Rekombinowany wektor adenowirusowy oraz sposób wytwarzania rekombinowanego wektora adenowirusowego
NZ539509A (en) 2002-10-23 2008-05-30 Glaxosmithkline Biolog Sa Priming vaccine comprising a polynucleotide encoding at least one first malarial antigen and a boosting vaccine comprising at least one polypeptide comprising at least one second malarial antigen having at least one epitope in common with the first malarial antigen of the priming vaccine
AP2351A (en) 2004-10-13 2012-01-25 Crucell Holland Bv Improved adenoviral vectors and uses thereof.
US20100143302A1 (en) 2006-03-16 2010-06-10 Crucell Holland B.V. Recombinant Adenoviruses Based on Serotype 26 and 48, and Use Thereof
EP2220217A2 (en) 2007-11-28 2010-08-25 The Trustees of the University of Pennsylvania Simian subfamily c adenoviruses sadv-40, -31, and-34 and uses thereof
CN101883858B (zh) 2007-11-28 2015-07-22 宾夕法尼亚大学托管会 猿猴亚家族E腺病毒SAdV-39、-25.2、-26、-30、-37和-38及其应用
CA2742474C (en) 2008-11-03 2016-05-31 Crucell Holland B.V. Method for the production of adenoviral vectors
AU2010209938A1 (en) 2009-02-02 2011-08-25 Glaxosmithkline Biologicals Sa Simian adenovirus nucleic acid- and amino acid-sequences, vectors containing same, and uses thereof
EA201270607A1 (ru) 2009-11-09 2012-09-28 Генвек, Инк. Аденовирус обезьян и способы его использования
DK2536829T3 (en) 2010-02-15 2016-07-04 Crucell Holland Bv A process for the production of Ad26-adenovirus vectors
TR201902214T4 (tr) 2010-04-16 2019-03-21 Glaxosmithkline Biologicals Sa Şempanze adenoviral vektörü-bazlı filovirüs aşıları.
WO2013016591A1 (en) * 2011-07-26 2013-01-31 Zuvachem, Inc. High efficiency isoprene synthases produced by protein engineering
CA2851251C (en) 2011-10-05 2023-09-12 Genvec, Inc. Simian (gorilla) adenovirus or adenoviral vectors and methods of use
EP2764011B1 (en) 2011-10-05 2021-04-07 GenVec, Inc. Adenoviral vector-based respiratory syncytial virus (rsv) vaccine
EP2809346A1 (en) 2012-02-02 2014-12-10 GenVec, Inc. Adenoviral vector-based malaria vaccine
SG10201609511XA (en) 2012-05-18 2016-12-29 Univ Pennsylvania Subfamily e simian adenoviruses a1302, a1320, a1331 and a1337 and uses thereof
EA038402B9 (ru) 2015-06-12 2021-09-22 Глаксосмитклайн Байолоджикалс Са Аденовирусные полинуклеотиды и полипептиды

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005071093A2 (en) * 2004-01-23 2005-08-04 Istituto Di Ricerche Di Biologia Molecolare P Angeletti Spa Chimpanzee adenovirus vaccine carriers
CN105112428A (zh) * 2009-02-02 2015-12-02 葛兰素史密斯克莱生物公司 猿腺病毒核酸和氨基酸序列，包含其的载体及其用途

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