CN111184835A

CN111184835A - A pharmaceutical composition prepared from baicalein for treating cervical cancer

Info

Publication number: CN111184835A
Application number: CN202010271836.0A
Authority: CN
Inventors: 喻小兰; 夏纪毅; 方俊; 张洪波; 谢鸿坤
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-04-09
Filing date: 2020-04-09
Publication date: 2020-05-22

Abstract

The invention belongs to the technical field of preparation of cervical cancer treatment medicines, and particularly relates to a composition of a cervical cancer treatment medicine prepared from baicalein, wherein a preparation method of the composition comprises the following steps: s1: preparing raw materials: weighing the following raw materials in parts by weight: 26-30 parts of baicalein extract, 12-16 parts of cortex dictamni, 8-12 parts of salvia miltiorrhiza, 4-8 parts of purple ramie, 6-10 parts of purple teak, 12-16 parts of oyster vegetable, 5-9 parts of adiantum fischeri, 3-7 parts of sharpleaf galangal fruit, 2-6 parts of Chinese magnoliavine fruit, 42-46 parts of gecko, 2-6 parts of hippocampi, 17-21 parts of giant hyssop, 16-20 parts of white paeony root and 15-19 parts of szechuan lovage rhizome for later use. The composition of the cervical cancer treatment medicine prepared by the invention can inhibit or kill cancer cells, has a good treatment effect on cervical cancer, has a remarkable effect, and is simple in the process of preparing the cervical cancer treatment medicine composition.

Description

A pharmaceutical composition prepared from baicalein for treating cervical cancer

Technical Field

The invention relates to the technical field of preparation of cervical cancer treatment medicines, in particular to a composition of a cervical cancer treatment medicine prepared from baicalein.

Background

Radix Scutellariae, also known as radix Camelliae Japonicae and radix Seu folium Elephantopi Japonicae, is a perennial herb of Scutellaria of Labiatae; fleshy rhizome is fat, leaf is solid and paper, the shape of the root is needle-shaped to linear needle-shaped, the general inflorescence grows on stems and branches, the corolla is purple, purplish red to blue, the filament is flat, the style of a flower is slender, the flower disc is annular, the ovary is brown, the small nut is in the egg ball shape, the period of the flower and the fruit is 7-9 months, the root of the radix scutellariae is used as the medicine, the taste is bitter, the nature is cold, and the effects of clearing heat and drying dampness, purging fire and removing toxicity, stopping bleeding, preventing. It can be used for treating epidemic febrile disease, upper respiratory infection, cough due to lung heat, yellow gallbladder due to damp-heat, pneumonia, dysentery, hemoptysis, conjunctival congestion, threatened abortion, hypertension, carbuncle, furuncle, and sore. The clinical antibacterial property of scutellaria is better than that of coptis, and the scutellaria does not produce drug resistance.

Cervical cancer refers to malignant tumor occurring at the junction of squamous epithelial cells of the cervicovaginal region or transitional zone and columnar epithelial cells of the endocervical lining, is the most common cervical malignant tumor in female reproductive system tumor, and has the main clinical manifestations of menorrhagia or leucorrhea, irregular vaginal bleeding, pain in waist and abdomen, anemia, emaciation and other systemic exhaustion symptoms. In recent years, the incidence of cervical cancer has been increasing, and the population suffering from cervical cancer is also gradually younger.

The traditional Chinese medicine is more and more concerned because of the advantages of resisting cancer and inhibiting swelling, having small toxic and side effects and the like, but the preparation process of the traditional cervical cancer treatment medicine composition is complicated, the preparation cost is high, and the treatment effect is poor.

Disclosure of Invention

The invention aims to solve the defects of complicated preparation process, high preparation cost and poor treatment effect of the cervical cancer treatment medicine composition in the prior art, and provides the cervical cancer treatment medicine composition prepared from baicalein.

In order to achieve the purpose, the invention adopts the following technical scheme:

a composition of a medicine prepared from baicalein and used for treating cervical cancer comprises the following raw materials in parts by weight: 26-30 parts of baicalein extract, 12-16 parts of cortex dictamni, 8-12 parts of salvia miltiorrhiza, 4-8 parts of purple ramie, 6-10 parts of purple teak, 12-16 parts of oyster vegetable, 5-9 parts of adiantum fischeri, 3-7 parts of sharpleaf galangal fruit, 2-6 parts of Chinese magnoliavine fruit, 42-46 parts of gecko, 2-6 parts of hippocampi, 17-21 parts of giant hyssop, 16-20 parts of white paeony root and 15-19 parts of szechuan lovage rhizome.

Preferably, the composition of the cervical cancer treatment medicine prepared from the baicalein comprises the following raw materials in parts by weight: 27-29 parts of baicalein extract, 13-15 parts of cortex dictamni, 9-11 parts of salvia miltiorrhiza, 5-7 parts of purple hemp, 7-9 parts of purple teak, 13-15 parts of oyster vegetable, 6-8 parts of adiantum fischeri, 4-6 parts of sharpleaf galangal fruit, 3-5 parts of Chinese magnoliavine fruit, 43-45 parts of gecko, 3-5 parts of hippocampi, 18-20 parts of giant hyssop, 17-19 parts of white paeony root and 16-18 parts of szechuan lovage rhizome.

Preferably, the composition of the cervical cancer treatment medicine prepared from the baicalein comprises the following raw materials in parts by weight: 28 parts of baicalein extract, 14 parts of dittany bark, 10 parts of salvia miltiorrhiza, 6 parts of purple ramie, 8 parts of purple teak, 14 parts of oyster vegetable, 7 parts of adiantum capillus-veneris, 5 parts of sharpleaf galangal fruit, 4 parts of Chinese magnoliavine fruit, 44 parts of gecko, 4 parts of hippocampus and hippocampus, 19 parts of giant salamander, 18 parts of white paeony root and 17 parts of szechuan lovage rhizome.

The invention also provides a preparation method of the composition of the cervical cancer treatment medicine prepared from the baicalein, which comprises the following steps:

s1: preparing raw materials: weighing the following raw materials in parts by weight: 26-30 parts of baicalein extract, 12-16 parts of cortex dictamni, 8-12 parts of salvia miltiorrhiza, 4-8 parts of purple ramie, 6-10 parts of purple teak, 12-16 parts of oyster vegetable, 5-9 parts of adiantum fischeri, 3-7 parts of sharpleaf galangal fruit, 2-6 parts of Chinese magnoliavine fruit, 42-46 parts of gecko, 2-6 parts of hippocampi, 17-21 parts of giant hyssop, 16-20 parts of white paeony root and 15-19 parts of szechuan lovage rhizome for later use;

s2: crushing: adding cortex Dictamni Radicis, Saviae Miltiorrhizae radix, radix Dactylicapni, fructus Citri Grandis, herba Lactucae Sativae, herba Adianti, fructus Alpinae Oxyphyllae, fructus Schisandrae chinensis, Gecko, Hippocampus, semen Sesami Niger, radix Paeoniae alba and rhizoma Ligustici Chuanxiong of S1 into a pulverizer, pulverizing, and sieving;

s3: mixing: adding the pulverized cortex dictamni, the salvia miltiorrhiza, the purple ramie, the purple teak, the oyster vegetables, the gelidium amansii, the sharpleaf galangal fruit, the schisandra chinensis, the gecko, the hippocampus, the jushenzi, the white paeony root and the ligusticum wallichii into a mixer for mixing treatment, and gradually adding the baicalein extract in the mixing process to obtain a mixture;

s4: fermentation: adding the mixture in the S3 into a fermentation tank, adding fermentation strains into the fermentation tank, and performing fermentation treatment;

s5: and (3) drying: and (4) putting the product obtained after fermentation in the S4 into a drying oven for drying treatment to obtain the cervical cancer treatment medicine composition.

Preferably, in the step S2, the rotation speed of the pulverizer is set to 200-300r/min, the temperature inside the pulverizer is set to 20-30 ℃, and after pulverization, the pulverized material is screened by using an 80-mesh sieve.

Preferably, in the S3, the rotation speed of the mixer is set to 300-400r/min, and the temperature inside the mixer is set to 15-20 ℃.

Preferably, in the step S4, the fermentation time is set to be 8-12 days, and the fermentation temperature is set to be 30-40 ℃.

Preferably, in S5, the temperature of the drying oven is set to 60-80 ℃, and the drying time is set to 30-60 min.

According to the composition of the medicine for treating the cervical cancer, which is prepared from the baicalein, the baicalein extract, the dittany bark, the red sage root, the purple hemp, the purple teak, the oyster vegetable, the adiantum capillus-veneris, the sharpleaf galangal fruit, the schisandra chinensis, the gecko, the hippocampus and the hippocampus, the giant salamander, the white paeony root and the ligusticum wallichii are selected, so that cancer cells can be inhibited or killed, tumors can be softened and reduced, and the cancer can be eliminated.

The composition of the cervical cancer treatment medicine prepared by the invention can inhibit or kill cancer cells, has a good treatment effect on cervical cancer, has a remarkable effect, and is simple in the process of preparing the cervical cancer treatment medicine composition.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments.

Example one

A composition of a medicine prepared from baicalein and used for treating cervical cancer comprises the following raw materials in parts by weight: 26 parts of baicalein extract, 12 parts of cortex dictamni, 8 parts of salvia miltiorrhiza, 4 parts of purple ramie, 6 parts of purple teak, 12 parts of oyster vegetable, 5 parts of adiantum capillus-veneris, 3 parts of sharpleaf galangal fruit, 2 parts of Chinese magnoliavine fruit, 42 parts of gecko, 2 parts of sea horse and sea horse, 17 parts of jushengzi, 16 parts of white paeony root and 15 parts of szechuan lovage rhizome.

The embodiment also provides a preparation method of the composition of the cervical cancer treatment medicine prepared from the baicalein, which comprises the following steps:

s1: preparing raw materials: weighing the following raw materials in parts by weight: 26 parts of baicalein extract, 12 parts of cortex dictamni, 8 parts of salvia miltiorrhiza, 4 parts of purple ramie, 6 parts of purple teak, 12 parts of oyster vegetable, 5 parts of adiantum capillus-veneris, 3 parts of sharpleaf galangal fruit, 2 parts of Chinese magnoliavine fruit, 42 parts of gecko, 2 parts of sea horse and sea horse, 17 parts of giant salamander, 16 parts of white paeony root and 15 parts of szechuan lovage rhizome for later use;

s2: crushing: adding cortex Dictamni Radicis, Saviae Miltiorrhizae radix, radix Dactylicapni, fructus Citri Grandis, herba Laciniae chinensis, herba Adianti, fructus Alpinae Oxyphyllae, fructus Schisandrae chinensis, Gecko, Hippocampus japonicus, semen Sesami Niger, radix Paeoniae alba and rhizoma Ligustici Chuanxiong in S1 into a pulverizer, pulverizing, and sieving;

In this example, in S2, the rotation speed of the pulverizer was set to 200r/min, the temperature inside the pulverizer was set to 20 ℃, after pulverization, the pulverized material was sieved using a 80-mesh sieve, in S3, the rotation speed of the mixer was set to 300r/min, the temperature inside the mixer was set to 15 ℃, in S4, the fermentation time was set to 8 days, the fermentation temperature was set to 30 ℃, in S5, the temperature of the drying oven was set to 60 ℃, and the drying time was set to 30 min.

Example two

A composition of a medicine prepared from baicalein and used for treating cervical cancer comprises the following raw materials in parts by weight: 27 parts of baicalein extract, 13 parts of cortex dictamni, 9 parts of salvia miltiorrhiza, 5 parts of purple ramie, 7 parts of purple teak, 13 parts of oyster vegetable, 6 parts of adiantum capillus-veneris, 4 parts of sharpleaf galangal fruit, 3 parts of Chinese magnoliavine fruit, 43 parts of gecko, 3 parts of hippocampus and hippocampus, 18 parts of jushengzi, 17 parts of white paeony root and 16 parts of szechuan lovage rhizome.

s1: preparing raw materials: weighing the following raw materials in parts by weight: 27 parts of baicalein extract, 13 parts of cortex dictamni, 9 parts of salvia miltiorrhiza, 5 parts of purple ramie, 7 parts of purple teak, 13 parts of oyster vegetable, 6 parts of adiantum capillus-veneris, 4 parts of sharpleaf galangal fruit, 3 parts of Chinese magnoliavine fruit, 43 parts of gecko, 3 parts of hippocampus and hippocampus, 18 parts of jushengzi, 17 parts of white paeony root and 16 parts of szechuan lovage rhizome for later use;

In this example, in S2, the rotation speed of the pulverizer was set to 220r/min, the temperature inside the pulverizer was set to 22 ℃, after pulverization, the pulverized material was sieved using a 80-mesh sieve, in S3, the rotation speed of the mixer was set to 320r/min, the temperature inside the mixer was set to 16 ℃, in S4, the fermentation time was set to 9 days, the fermentation temperature was set to 32 ℃, in S5, the temperature of the drying oven was set to 64 ℃, and the drying time was set to 32 min.

EXAMPLE III

A composition of a medicine prepared from baicalein and used for treating cervical cancer comprises the following raw materials in parts by weight: 28 parts of baicalein extract, 14 parts of dittany bark, 10 parts of salvia miltiorrhiza, 6 parts of purple ramie, 8 parts of purple teak, 14 parts of oyster vegetable, 7 parts of adiantum capillus-veneris, 5 parts of sharpleaf galangal fruit, 4 parts of Chinese magnoliavine fruit, 44 parts of gecko, 4 parts of hippocampus and hippocampus, 19 parts of giant salamander, 18 parts of white paeony root and 17 parts of szechuan lovage rhizome.

s1: preparing raw materials: weighing the following raw materials in parts by weight: 28 parts of baicalein extract, 14 parts of dittany bark, 10 parts of salvia miltiorrhiza, 6 parts of purple ramie, 8 parts of purple teak, 14 parts of oyster vegetable, 7 parts of adiantum capillus-veneris, 5 parts of sharpleaf galangal fruit, 4 parts of Chinese magnoliavine fruit, 44 parts of gecko, 4 parts of sea horse and sea horse, 19 parts of giant salamander, 18 parts of white paeony root and 17 parts of szechuan lovage rhizome for later use;

In this example, in S2, the rotation speed of the pulverizer was set to 250r/min, the temperature inside the pulverizer was set to 25 ℃, after pulverization, the pulverized material was sieved using a 80-mesh sieve, in S3, the rotation speed of the mixer was set to 350r/min, the temperature inside the mixer was set to 17 ℃, in S4, the fermentation time was set to 10 days, the fermentation temperature was set to 35 ℃, in S5, the temperature of the drying oven was set to 70 ℃, and the drying time was set to 45 min.

Example four

A composition of a medicine prepared from baicalein and used for treating cervical cancer comprises the following raw materials in parts by weight: 29 parts of baicalein extract, 15 parts of dittany bark, 11 parts of salvia miltiorrhiza, 7 parts of purple ramie, 9 parts of purple teak, 15 parts of oyster vegetable, 8 parts of adiantum capillus-veneris, 6 parts of sharpleaf galangal fruit, 5 parts of Chinese magnoliavine fruit, 45 parts of gecko, 5 parts of sea horse and sea horse, 20 parts of jushengzi, 19 parts of white paeony root and 18 parts of szechuan lovage rhizome.

s1: preparing raw materials: weighing the following raw materials in parts by weight: 29 parts of baicalein extract, 15 parts of dittany bark, 11 parts of salvia miltiorrhiza, 7 parts of purple ramie, 9 parts of purple teak, 15 parts of oyster vegetable, 8 parts of adiantum capillus-veneris, 6 parts of sharpleaf galangal fruit, 5 parts of Chinese magnoliavine fruit, 45 parts of gecko, 5 parts of sea horse and sea horse, 20 parts of giant salamander, 19 parts of white paeony root and 18 parts of szechuan lovage rhizome for later use;

In this example, in S2, the rotation speed of the pulverizer was set to 280r/min, the temperature inside the pulverizer was set to 28 ℃, after pulverization, the pulverized material was sieved using a 80-mesh sieve, in S3, the rotation speed of the mixer was set to 380r/min, the temperature inside the mixer was set to 19 ℃, in S4, the fermentation time was set to 11 days, the fermentation temperature was set to 38 ℃, in S5, the temperature of the drying oven was set to 76 ℃, and the drying time was set to 55 min.

EXAMPLE five

A composition of a medicine prepared from baicalein and used for treating cervical cancer comprises the following raw materials in parts by weight: 30 parts of baicalein extract, 16 parts of cortex dictamni, 12 parts of salvia miltiorrhiza, 8 parts of purple ramie, 10 parts of purple teak, 16 parts of oyster vegetable, 9 parts of adiantum capillus-veneris, 7 parts of sharpleaf galangal fruit, 6 parts of Chinese magnoliavine fruit, 46 parts of gecko, 6 parts of hippocampus and hippocampus, 21 parts of jushengzi, 20 parts of white paeony root and 19 parts of szechuan lovage rhizome.

s1: preparing raw materials: weighing the following raw materials in parts by weight: 30 parts of baicalein extract, 16 parts of cortex dictamni, 12 parts of salvia miltiorrhiza, 8 parts of purple ramie, 10 parts of purple teak, 16 parts of oyster vegetable, 9 parts of adiantum capillus-veneris, 7 parts of sharpleaf galangal fruit, 6 parts of Chinese magnoliavine fruit, 46 parts of gecko, 6 parts of sea horse and sea horse, 21 parts of giant salamander, 20 parts of white paeony root and 19 parts of szechuan lovage rhizome for later use;

In this example, in S2, the rotation speed of the pulverizer was set to 300r/min, the temperature inside the pulverizer was set to 30 ℃, after pulverization, the pulverized material was sieved using an 80-mesh sieve, in S3, the rotation speed of the mixer was set to 400r/min, the temperature inside the mixer was set to 20 ℃, in S4, the fermentation time was set to 12 days, the fermentation temperature was set to 40 ℃, in S5, the temperature of the drying oven was set to 80 ℃, and the drying time was set to 60 min.

Experimental study for treatment of cervical cancer:

selecting 60 advanced cervical cancer patients, wherein the ages of the patients are 40-60 years, 10 patients are taken as one group, dividing the group into six groups, and the patients in a control group receive regular radiotherapy and standard chemotherapy; the treatment group added a composition of a cervical cancer treatment drug prepared from baicalein on the basis of patients receiving regular radiotherapy and standard chemotherapy, and the composition was taken twice a day by 1g for two consecutive treatment courses, each treatment course lasting 7 days, to obtain the following results:

grouping	Is effective	Invalidation	Effective ratio of
				Control group	3	7	30%
Example one treatment group	7	3	70%
				EXAMPLE two treatment groups	8	2	80%
EXAMPLE three treatment groups	6	4	60%
				EXAMPLE four treatment groups	6	4	60%
EXAMPLE five treatment groups	7	3	70%

The results show that the composition of the cervical cancer treatment medicine prepared from the baicalein can inhibit or kill cancer cells on the basis of normal radiotherapy and standard chemotherapy of patients, has a good treatment effect on cervical cancer, has a remarkable effect, and the second embodiment is the best embodiment.

The following is further illustrated by some practical examples:

the patient Wangzhi, female, age 44, menstruation is normal, excessive bleeding after the same room is confirmed to be cervical squamous carcinoma after cytological examination, gynecological examination and cervical pathological biopsy, the effect is not obvious after the medicine is taken, the composition of the cervical cancer treatment medicine prepared from baicalein prepared in the third embodiment of the invention is taken later, 1g is taken twice a day, and the normal state is recovered after half a year of examination;

patients who have got Liu somebody, women, 50 years old, were admitted to the hospital for one year of contact bleeding, the plain menstruation rule of patients, 5-6/30 days, moderate, no dysmenorrhea, and contact bleeding started in last august. In the last year, menstruation is irregular, the amount is large in 4-5/24-15 days, and the sanitary towel is 10+ bags/month. The hospital in the last decade is not in detail. The cervical cancer is shown in cervical biopsy after the early half month due to more menstrual flow in an outpatient clinic, the major bleeding appears after the cervical biopsy, the systemic chemotherapy is combined with the uterine artery chemotherapy in 17 days after 6 months, and the postoperative bleeding is obviously reduced in embolization. Requiring the operation to be admitted to the hospital. The patients have no dizziness, nausea, vomiting, abdominal pain and feeling of anal tenesmus in the course of disease, the curative effect of the phosphorus cell carcinoma of cervix confirmed after further diagnosis and treatment is poor after taking the medicine for 3 years, and the medicine composition prepared by the fifth embodiment of the invention is taken after the medicine is taken, and the related symptoms disappear after the medicine is treated for 8 months;

the patient has vaginal fluid of unknown reason and peculiar smell since Zhao-somewhat, female, 55 years old and half a month. When the patient visits the local hospital, gynecological examination finds that the cervix has vegetable-like tumors and has contact bleeding, and biopsy pathology indicates that squamous cell carcinoma is differentiated in the cervix. In order to further diagnose and treat the disease, the patients have normal diet and defecation since the disease occurs. Has no body weight, no lumbago, frequent micturition, and odynuria. Without difficulty in defecation and bloody stool. The patients are in clinic and gynecological examination shows that the diameter of cervical tumor is about 4cm, and the exogenous vegetable phyma is formed. The pathological consultation of the hospital indicates that squamous cell carcinoma is differentiated, the curative effect of the medicine is poor, and the medicine composition prepared by the first embodiment of the invention is taken later, and the related symptoms disappear after 10 months of treatment.

Claims

1. A composition of a medicine prepared from baicalein and used for treating cervical cancer is characterized by comprising the following raw materials in parts by weight: 26-30 parts of baicalein extract, 12-16 parts of cortex dictamni, 8-12 parts of salvia miltiorrhiza, 4-8 parts of purple ramie, 6-10 parts of purple teak, 12-16 parts of oyster vegetable, 5-9 parts of adiantum fischeri, 3-7 parts of sharpleaf galangal fruit, 2-6 parts of Chinese magnoliavine fruit, 42-46 parts of gecko, 2-6 parts of hippocampi, 17-21 parts of giant hyssop, 16-20 parts of white paeony root and 15-19 parts of szechuan lovage rhizome.

2. The composition of the medicine for treating the cervical cancer, which is prepared from the baicalein, according to the claim 1, is characterized by comprising the following raw materials in parts by weight: 27-29 parts of baicalein extract, 13-15 parts of cortex dictamni, 9-11 parts of salvia miltiorrhiza, 5-7 parts of purple hemp, 7-9 parts of purple teak, 13-15 parts of oyster vegetable, 6-8 parts of adiantum fischeri, 4-6 parts of sharpleaf galangal fruit, 3-5 parts of Chinese magnoliavine fruit, 43-45 parts of gecko, 3-5 parts of hippocampi, 18-20 parts of giant hyssop, 17-19 parts of white paeony root and 16-18 parts of szechuan lovage rhizome.

3. The composition of the medicine for treating the cervical cancer, which is prepared from the baicalein, according to the claim 1, is characterized by comprising the following raw materials in parts by weight: 28 parts of baicalein extract, 14 parts of dittany bark, 10 parts of salvia miltiorrhiza, 6 parts of purple ramie, 8 parts of purple teak, 14 parts of oyster vegetable, 7 parts of adiantum capillus-veneris, 5 parts of sharpleaf galangal fruit, 4 parts of Chinese magnoliavine fruit, 44 parts of gecko, 4 parts of hippocampus and hippocampus, 19 parts of giant salamander, 18 parts of white paeony root and 17 parts of szechuan lovage rhizome.

4. A preparation method of a composition of a cervical cancer treatment medicine prepared from baicalein is characterized by comprising the following steps:

5. The method for preparing a pharmaceutical composition for the treatment of cervical cancer comprising baicalein as claimed in claim 4, wherein in S2, the rotation speed of the pulverizer is set at 200-300r/min, the temperature inside the pulverizer is set at 20-30 ℃, and after pulverization, the pulverized material is screened with a 80 mesh sieve.

6. The method for preparing a composition of a drug for the treatment of cervical cancer from baicalein as claimed in claim 4, wherein in S3, the rotation speed of the mixer is set to 300-400r/min, and the temperature inside the mixer is set to 15-20 ℃.

7. The method for preparing a pharmaceutical composition for the treatment of cervical cancer comprising baicalein according to claim 4, wherein the fermentation time is set to 8-12 days and the fermentation temperature is set to 30-40 ℃ in S4.

8. The method of preparing a pharmaceutical composition for the treatment of cervical cancer using baicalein according to claim 4, wherein the temperature of the drying oven is set to 60-80 ℃ and the drying time is set to 30-60min in S5.