CN105287805A - Traditional Chinese medicine combination extract and application thereof in preparation of medicines for treating cervical carcinoma - Google Patents
Traditional Chinese medicine combination extract and application thereof in preparation of medicines for treating cervical carcinoma Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine combination extract and application thereof in preparation of medicines for treating cervical carcinoma. The traditional Chinese medicine combination extract is prepared from lysimachia candida, cortex dictamni, arcangelisia loureiri and red sage root through the following steps: cleaning all raw materials, airing, then crushing and mixing, collecting extracted volatile oil by adopting a supercritical carbon dioxide extraction technology, carrying out warm-infusion extraction with ethanol, collecting an extracting solution, adding water for reflux extraction, and separating and purifying the extracting solution with a liquid chromatograph, thereby obtaining the target traditional Chinese medicine composition. The traditional Chinese medicine combination extract serves as an active ingredient to be applied to preparation of medicines for treating cervical carcinoma in a pharmaceutical composition accepting additives in pharmaceutics. All components in the medicine disclosed by the invention are synergistic and have a good therapeutic effect on cervical carcinoma. The invention provides a new medicine for patients with cervical carcinoma, and the medicine is small in toxic or side effect and free of adverse reaction.
Description
Technical field
The present invention relates to technical field of Chinese medicines, specifically a kind of Chinese medicine composition extract and the application in preparation treatment cervical cancer medicine thereof.
Background technology
Cervical cancer refers to the malignant tumor of the columnar epithelial cell intersection of squamous cell and the neck tube inner membrance occurring in ectocervical or transitional zone, cervical cancer is uterine cervix malignant tumor the most common in feminine proses, main clinical manifestation is menstruation or leukorrhagia, abnormal vaginal bleeding, waist and belly pain, anemia, the cachexia symptom such as to become thin.In recent years, the sickness rate of cervical cancer continues to rise, and morbidity crowd is also developed to rejuvenation gradually.Long-term clinical point thinks that the sensitivity of Chemotherapy of Cervical Cancer is poor, effective percentage is all lower than 15%, the chemotherapeutics of current cervical cancer can adopt 5-fluorine to urinate crash steep, amycin etc. to carry out vein or local injection, but toxicity of adriamycin reaction is heavier, and carcinogenesis is found in zoopery, in human body, also there is potential carcinogenic, mutagenic action, have significant impact to reproductive function.And current operation plan excises cancerous issue and chemicotherapy, not only cure rate is low, and excessive to the actual bodily harm of women.It is little that treatment by Chinese herbs has side effect, the advantage that anti-recurrence rate is effective, can stablize the state of an illness, indiffusion, part Chinese medicine formula Clinical Practice, and therapeutic effect is not very desirable, is therefore badly in need of the cervical cancer treatment by Chinese herbs medicine seeking a kind of low toxicity, Be very effective.
Summary of the invention
The object of the present invention is to provide a kind of Chinese medicine composition extract and the application in preparation treatment cervical cancer medicine thereof, to solve the problem proposed in above-mentioned background technology.
For achieving the above object, the invention provides following technical scheme:
A kind of Chinese medicine composition extract, is prepared from by the raw material of following weight portion: Herba Lysimachiae Candidae 30 ~ 40 parts, Cortex Dictamni 15 ~ 19 parts, Caulis Arcangelisiae 16 ~ 20 parts, Radix Salviae Miltiorrhizae 21 ~ 25 parts.
A preparation method for described Chinese medicine composition extract, concrete preparation process is as follows:
(1) above-mentioned each raw material is cleaned respectively dry, take each raw material by proportioning, after pulverizing with pulverizer respectively, cross 100 ~ 120 mesh sieves, abundant mix homogeneously;
(2) raw material of mixing is adopted supercritical carbon dioxide extraction method, extraction temperature 54 ~ 60 DEG C, extracting pressure 6 ~ 8MPa, extraction time 2 ~ 3h, collect the volatile oil of extraction, obtain extract A, for subsequent use;
(3) be solvent by extract A concentration 90 ~ 95% ethanol, at 75 ~ 80 DEG C, warm macerating extracts, extraction time is 4 ~ 6 times, each extraction time is 2 ~ 3h, and each solvent load is 10 ~ 12 times of raw material gross weight, filters, collect extracting solution, extracting solution reclaims ethanol, and concentrate drying, obtains extract B;
(4) add water extract B reflux, extract, and extraction time is 3 ~ 5 times, and each extraction time is 2 ~ 3h, and each water consumption is 7 ~ 9 times of raw material gross weight, filters, obtain extracting solution;
(5) pump in ultrasonic standing wave preparative liquid chromatogram by extracting solution, ultrasonic standing wave power 3.0 ~ 5.OKW, frequency 500 ~ 1000kHz, flow velocity 50 ~ 500mL/min, UV-detector wavelength 280nm, separation and purification, obtains target Chinese medicine composition.
A kind of described Chinese medicine composition extract accepts the application of pharmaceutical composition in preparation treatment cervical cancer medicine of additives on pharmacopedics as active component.
As the further scheme of the present invention: described treatment cervical cancer medicine makes solid preparation or liquid preparation, and described solid preparation is granule, tablet, drop pill, injectable powder or capsule, and liquid preparation is oral liquid.
As the present invention's further scheme: in described solid preparation, the mass content of this combined extracts is 5 ~ 7%, in liquid preparation, the mass content of Cyclocarya paliurus Iljinskaja extract is 3 ~ 5%.
Compared with prior art, the invention has the beneficial effects as follows: each component synergism in medicine of the present invention, has good therapeutical effect to cervical cancer, Be very effective, and provide a kind of new drug to vast cervix uteri patient, toxic and side effects is little, has no adverse reaction.
Detailed description of the invention
Below in conjunction with the embodiment of the present invention, be clearly and completely described the technical scheme in the embodiment of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
Embodiment 1
Chinese medicine composition extract, is prepared from by the raw material of following weight portion: Herba Lysimachiae Candidae 30 parts, Cortex Dictamni 19 parts, Caulis Arcangelisiae 16 parts, Radix Salviae Miltiorrhizae 25 parts.
The preparation method of above-mentioned Chinese medicine composition extract, concrete preparation process is as follows:
(1) above-mentioned each raw material is cleaned respectively dry, take each raw material by proportioning, after pulverizing with pulverizer respectively, cross 100 mesh sieves, abundant mix homogeneously;
(2) raw material of mixing is adopted supercritical carbon dioxide extraction method, extraction temperature 57 DEG C, extracting pressure 7MPa, extraction time 3h, collect the volatile oil of extraction, obtain extract A, for subsequent use;
(3) be solvent by extract A concentration 95% ethanol, at 80 DEG C, warm macerating extracts, and extraction time is 5 times, and each extraction time is 2h, each solvent load is 11 times of raw material gross weight, filters, and collects extracting solution, extracting solution reclaims ethanol, and concentrate drying, obtains extract B;
(4) add water extract B reflux, extract, and extraction time is 4 times, and each extraction time is 2h, and each water consumption is 8 times of raw material gross weight, filters, obtain extracting solution;
(5) pump in ultrasonic standing wave preparative liquid chromatogram by extracting solution, ultrasonic standing wave power 4.0KW, frequency 750kHz, flow velocity 150mL/min, UV-detector wavelength 280nm, separation and purification, obtains target Chinese medicine composition.
Embodiment 2
Chinese medicine composition extract, is prepared from by the raw material of following weight portion: Herba Lysimachiae Candidae 35 parts, Cortex Dictamni 17 parts, Caulis Arcangelisiae 18 parts, Radix Salviae Miltiorrhizae 23 parts.
The preparation method of above-mentioned Chinese medicine composition extract, concrete preparation process is as follows:
(1) above-mentioned each raw material is cleaned respectively dry, take each raw material by proportioning, after pulverizing with pulverizer respectively, cross 110 mesh sieves, abundant mix homogeneously;
(2) raw material of mixing is adopted supercritical carbon dioxide extraction method, extraction temperature 60 DEG C, extracting pressure 7MPa, extraction time 2h, collect the volatile oil of extraction, obtain extract A, for subsequent use;
(3) be solvent by extract A concentration 95% ethanol, at 80 DEG C, warm macerating extracts, and extraction time is 5 times, and each extraction time is 2h, each solvent load is 11 times of raw material gross weight, filters, and collects extracting solution, extracting solution reclaims ethanol, and concentrate drying, obtains extract B;
(4) add water extract B reflux, extract, and extraction time is 4 times, and each extraction time is 3h, and each water consumption is 8 times of raw material gross weight, filters, obtain extracting solution
(5) pump in ultrasonic standing wave preparative liquid chromatogram by extracting solution, ultrasonic standing wave power 4.0KW, frequency 750kHz, flow velocity 200mL/min, UV-detector wavelength 280nm, separation and purification, obtains target Chinese medicine composition.
Embodiment 3
Chinese medicine composition extract, is prepared from by the raw material of following weight portion: Herba Lysimachiae Candidae 40 parts, Cortex Dictamni 15 parts, Caulis Arcangelisiae 20 parts, Radix Salviae Miltiorrhizae 21 parts.
The preparation method of above-mentioned Chinese medicine composition extract, concrete preparation process is as follows:
(1) above-mentioned each raw material is cleaned respectively dry, take each raw material by proportioning, after pulverizing with pulverizer respectively, cross 120 mesh sieves, abundant mix homogeneously;
(2) raw material of mixing is adopted supercritical carbon dioxide extraction method, extraction temperature 60 DEG C, extracting pressure 7MPa, extraction time 3h, collect the volatile oil of extraction, obtain extract A, for subsequent use;
(3) be solvent by extract A concentration 95% ethanol, at 80 DEG C, warm macerating extracts, and extraction time is 5 times, and each extraction time is 2h, each solvent load is 11 times of raw material gross weight, filters, and collects extracting solution, extracting solution reclaims ethanol, and concentrate drying, obtains extract B;
(4) add water extract B reflux, extract, and extraction time is 4 times, and each extraction time is 3h, and each water consumption is 8 times of raw material gross weight, filters, obtain extracting solution;
(5) pump in ultrasonic standing wave preparative liquid chromatogram by extracting solution, ultrasonic standing wave power 4.0KW, frequency 750Hz, flow velocity 250mL/min, UV-detector wavelength 280nm, separation and purification, obtains target Chinese medicine composition.
Comparative example 1
Chinese medicine composition extract, is prepared from by the raw material of following weight portion: Herba Lysimachiae Candidae 35 parts, Cortex Dictamni 17 parts, Caulis Arcangelisiae 18 parts.Preparation method is with embodiment 2.
Comparative example 2
Chinese medicine composition extract, is prepared from by the raw material of following weight portion: Herba Lysimachiae Candidae 35 parts, Cortex Dictamni 17 parts, Radix Salviae Miltiorrhizae 23 parts.Preparation method is with embodiment 2.
Comparative example 3
Chinese medicine composition extract, is prepared from by the raw material of following weight portion: Herba Lysimachiae Candidae 35 parts, Caulis Arcangelisiae 18 parts, Radix Salviae Miltiorrhizae 23 parts.Preparation method is with embodiment 2.
Comparative example 4
Chinese medicine composition extract, is prepared from by the raw material of following weight portion: Cortex Dictamni 17 parts, Caulis Arcangelisiae 18 parts, Radix Salviae Miltiorrhizae 23 parts.Preparation method is with embodiment 2.
Comparative example 5
Chinese medicine composition extract, is prepared from by the raw material of following weight portion: Herba Lysimachiae Candidae 35 parts, and Cortex Dictamni 17 parts, preparation method is with embodiment 2.
Comparative example 6
Chinese medicine composition extract, is prepared from by the raw material of following weight portion: Caulis Arcangelisiae 18 parts, Radix Salviae Miltiorrhizae 23 parts.Preparation method is with embodiment 2.
Comparative example 7
Chinese medicine composition extract, is prepared from by the raw material of following weight portion: Herba Lysimachiae Candidae 35 parts, Caulis Arcangelisiae 18 parts.Preparation method is with embodiment 2.
Comparative example 8
Chinese medicine composition extract, is prepared from by the raw material of following weight portion: Cortex Dictamni 17 parts, Radix Salviae Miltiorrhizae 23 parts.Preparation method is with embodiment 2.
The anti-human s active testing of test example two pharmaceutical composition of the present invention
Part I: anti tumor activity in vitro method of testing
A, principle: the succinum dehydrogenase in living cells plastochondria can make exogenous tetrazolium bromide (MTT) be reduced to water-insoluble bluish violet crystallization first a ceremonial jade-ladle, used in libation and be deposited in cell, and dead cell is without this function.First a ceremonial jade-ladle, used in libation in dimethyl sulfoxide energy dissolved cell, measures its absorbance value with enzyme-linked immunosorbent assay instrument, can indirectly reflect living cells quantity.
B, cell: human cervical carcinoma Hela cell's strain (Chinese Academy of Sciences's Shanghai school of life and health sciences cell bank).
The preparation of C, sample:
(1) the Chinese medicine composition extract lmg DMSO of 40 μ L is dissolved the mother solution being configured to 25mg/mL, use DMEM culture medium (containing 800,000 units of Penicillin in every 1000mL culture medium again, 1.0g streptomycin, 10% inactivated fetal bovine serum) serial dilution becomes Chinese medicine composition extract concentrations to be 1000 μ g/mL, 100 μ g/mL, 10 μ g/mL, l μ g/mL, the sample liquid of 0.1 μ/mL.
(2) each for embodiment 1-3 and comparative example 1-8 assembly being made concentration is 1000 μ g/mL, 100 μ g/mL, 10 μ g/mL, l μ g/mL, the sample liquid of 0.1 μ g/mL.
(3) using identical method to prepare 5-fluorouracil concentration is 1000 μ g/mL, 100 μ g/mL, 10 μ g/mL, l μ g/mL, the sample liquid of 0.1 μ g/mL.
D, experimental procedure:
MTT tests: one bottle, trophophase Hela cell of taking the logarithm, and abandons culture fluid, cell EDTA-trypsinization liquid is digested about 15s in 37 DEG C of incubators, postdigestive cell DMEM culture medium is diluted to 1 × 10
6individual/mL, is added in 96 porocyte culture plates, every hole 100 μ L.Blank group, experiment contrast group, positive controls (5-fluorouracil) and Chinese medicine composition extract group are established in experiment, and often group establishes 3 parallel holes, puts 37 DEG C, 5%C0
2after cultivating 24h in incubator, add the sample liquid of above-mentioned preparation, every hole 100 μ L, experiment contrast group only adds culture fluid, the blank group that do not add cell only add culture fluid parallel with experiment contrast group.Put 37 DEG C, 5%CO
2after cultivating 72h in incubator, the MTT solution of 5mg/mL is added in cell culture well, every hole lO μ L, put 37 DEG C and hatch 4h, add DMSO, every hole 150 μ L, with oscillator vibrates, first a ceremonial jade-ladle, used in libation is dissolved completely, by microplate reader (XD-711, upper Hisoon reaches Medical Devices Co., Ltd.'s product) colorimetric under 570nm wavelength.With similarity condition with containing sample, containing the culture medium culturing of same concentration DMSO cell in contrast.Experiment repetition 3 times, gets its meansigma methods as experimental result.According to the half-inhibition concentration (IC of LOGIT method calculation sample to growth of tumour cell
50).
Growth of tumour cell suppression ratio (CI)=[l-(Chinese medicine composition extract group OD value-blank group OD value)/(experiment contrast group OD value-blank group OD value)] × 100%.
E, experimental result: anti-human s active testing the results are shown in Table shown in 1.
| Group | IC 50(μg/mL) |
| Embodiment 1 | 9.21 |
| Embodiment 2 | 8.82 |
| Embodiment 3 | 9.16 |
| Comparative example 1 | 15.20 |
| Comparative example 2 | 16.05 |
| Comparative example 3 | 15.75 |
| Comparative example 4 | 15.86 |
| Comparative example 5 | 32.02 |
| Comparative example 6 | 31.86 |
| Comparative example 7 | 30.89 |
| Comparative example 8 | 31.48 |
| 5-fluorouracil | 25.62 |
Table 1 can be found out, according to evaluation criterion (the Compound I C of anti-tumor activity
50< 10 μ g/mL, then judgement sample has lethal effect to tumor cell in vitro; IC
50< 30 μ g/mL, then judgement sample has tumor cell in vitro and has stronger inhibited proliferation), embodiment 1-3 group has stronger inhibited proliferation to human cervical carcinoma Hela cell, and be obviously better than comparative example 1-8, illustrate that the inhibited proliferation of the synergism of each medicine in embodiment 1-3 group to tumor cell is the strongest,, therefore, Chinese medicine composition extract of the present invention can be used for the medicine preparing treatment cervical cancer.
Part II: zoopery
1 materials and methods
1.1 materials and reagent
Human cervical carcinoma Hela cell's strain is purchased from Chinese Academy of Sciences's Shanghai school of life and health sciences cell bank, and RPMI-1640 (Gibco), newborn calf serum are purchased from Hangzhou Ligustrum malongense B. S. Sun company; Mutantion line no-special pathogen (SPF) level Balb/c-nu mice 120,4-6 week age, female, body weight 14-18g, raise in aseptic shielding environment.
1.2 human cervical carcinoma's transplanted tumor in nude mice Experiment on therapy
Cultivate based on 370 DEG C with the RPMI-1640 containing 10% calf serum, cultivate under 5% carbon dioxide saturated humidity; Cell attachment is grown in culture medium, and trophophase cell of taking the logarithm is for experiment.In nude mice dorsal sc injection Hela cell 2 × 10
6individual/only.Treat that subcutaneous transplantation tumor volume reaches 75mm
3time (about 30d), be divided into 12 groups by tumor volume and mice with tumor body weight homeostatic principle, often organize 10.
Model control group: the normal saline of tail vein injection equivalent;
Chinese medicine composition extract embodiment 1;
Chinese medicine composition extract embodiment 2;
Chinese medicine composition extract embodiment 3;
Comparative example 1-8;
The next day administration 1 time, amount to 8 times, during administration every 4 days with the most major diameter (L) of vernier caliper measurement transplanted tumor and most minor axis (W).After last administration 48h, mice is put to death in dislocation, and excision transplanted tumor, takes tumor weight.Tumor-like hyperplasia (%) IR=(1-experimental group tumor weight-average value/matched group tumor weight-average value) × 100%, by tumor heavy relatively embody the impact that medicine grows human cervical carcinoma Hela cell's transplanted tumor in nude mice.
1.3 statistical procedures
Data represent with mean scholar standard deviation, adopt SPSS15.0 software to carry out variance analysis.
2 results
Result of the test shows, in table 2.
The impact that table 2 pharmaceutical composition of the present invention grows HeLa Cells transplanted tumor in nude mice
| Group | Tumor weight/mg | Tumour inhibiting rate/% |
| Embodiment 1 | 52±18 | 86.77 |
| Embodiment 2 | 48±15 | 87.79 |
| Embodiment 3 | 56±12 | 85.75 |
| Comparative example 1 | 322±30 | 18.07 |
| Comparative example 2 | 318±20 | 19.08 |
| Comparative example 3 | 320±26 | 18.58 |
| Comparative example 4 | 315±15 | 19.85 |
| Comparative example 5 | 368±24 | 6.36 |
| Comparative example 6 | 370±32 | 5.85 |
| Comparative example 7 | 369±26 | 6.11 |
| Comparative example 8 | 375±35 | 4.58 |
| Model control group | 393±56 | -- |
(1) compared with model control group, embodiment 1-3 group all has pole significant difference to the inhibitory action that HeLa Cells transplanted tumor in nude mice grows, and illustrates that Chinese medicine composition extract of the present invention can effectively suppress HeLa Cells transplanted tumor in nude mice to grow.
(2) compared with comparative example 1-8 group, embodiment 1-3 group has significant difference (p < 0.01) to the inhibitory action that HeLa Cells transplanted tumor in nude mice grows, and illustrates that the drug effect of Chinese medicine composition extract of the present invention has significant synergism.
(3) adverse reaction rate of each administration group has been investigated in this test simultaneously, and by such as suffering from diarrhoea to test mice toxic and side effects, lethargy etc. observes and find, compared with comparative example 1-8, embodiment 1-3 group has no adverse reaction.
To those skilled in the art, obviously the invention is not restricted to the details of above-mentioned one exemplary embodiment, and when not deviating from spirit of the present invention or basic feature, the present invention can be realized in other specific forms.Therefore, no matter from which point, all should embodiment be regarded as exemplary, and be nonrestrictive, scope of the present invention is limited by claims instead of above-mentioned explanation, and all changes be therefore intended in the implication of the equivalency by dropping on claim and scope are included in the present invention.
Claims (5)
1. a Chinese medicine composition extract, is characterized in that, is prepared from by the raw material of following weight portion: Herba Lysimachiae Candidae 30 ~ 40 parts, Cortex Dictamni 15 ~ 19 parts, Caulis Arcangelisiae 16 ~ 20 parts, Radix Salviae Miltiorrhizae 21 ~ 25 parts.
2. a preparation method for Chinese medicine composition extract as claimed in claim 1, is characterized in that, concrete preparation process is as follows:
(1) above-mentioned each raw material is cleaned respectively dry, take each raw material by proportioning, after pulverizing with pulverizer respectively, cross 100 ~ 120 mesh sieves, abundant mix homogeneously;
(2) raw material of mixing is adopted supercritical carbon dioxide extraction method, extraction temperature 54 ~ 60 DEG C, extracting pressure 6 ~ 8MPa, extraction time 2 ~ 3h, collect the volatile oil of extraction, obtain extract A, for subsequent use;
(3) be solvent by extract A concentration 90 ~ 95% ethanol, at 75 ~ 80 DEG C, warm macerating extracts, extraction time is 4 ~ 6 times, each extraction time is 2 ~ 3h, and each solvent load is 10 ~ 12 times of raw material gross weight, filters, collect extracting solution, extracting solution reclaims ethanol, and concentrate drying, obtains extract B;
(4) add water extract B reflux, extract, and extraction time is 3 ~ 5 times, and each extraction time is 2 ~ 3h, and each water consumption is 7 ~ 9 times of raw material gross weight, filters, obtain extracting solution;
(5) pump in ultrasonic standing wave preparative liquid chromatogram by extracting solution, ultrasonic standing wave power 3.0 ~ 5.OKW, frequency 500 ~ 1000kHz, flow velocity 50 ~ 500mL/min, UV-detector wavelength 280nm, separation and purification, obtains target Chinese medicine composition.
3. a Chinese medicine composition extract as claimed in claim 1 accepts the application of pharmaceutical composition in preparation treatment cervical cancer medicine of additives on pharmacopedics as active component.
4. application according to claim 3, is characterized in that, described treatment cervical cancer medicine makes solid preparation or liquid preparation, and described solid preparation is granule, tablet, drop pill, injectable powder or capsule, and liquid preparation is oral liquid.
5. application according to claim 4, is characterized in that, in described solid preparation, the mass content of this combined extracts is 5 ~ 7%, and in liquid preparation, the mass content of Cyclocarya paliurus Iljinskaja extract is 3 ~ 5%.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111184835A (en) * | 2020-04-09 | 2020-05-22 | 喻小兰 | A pharmaceutical composition prepared from baicalein for treating cervical cancer |
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| CN101125178A (en) * | 2007-08-27 | 2008-02-20 | 毕哲全 | Traditional Chinese medicine for treating tumor |
| CN104524372A (en) * | 2014-12-30 | 2015-04-22 | 范士怀 | Traditional Chinese medicine preparation for treating cervical cancer |
| CN104888149A (en) * | 2015-06-27 | 2015-09-09 | 刘今方 | Drug for treating cervical cancer and preparation method for drug |
| CN104906558A (en) * | 2015-07-08 | 2015-09-16 | 广东天普生化医药股份有限公司 | Application of ulinastatin for preparing cervical cancer treatment medicine and pharmaceutical composition |
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2015
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101125178A (en) * | 2007-08-27 | 2008-02-20 | 毕哲全 | Traditional Chinese medicine for treating tumor |
| CN104524372A (en) * | 2014-12-30 | 2015-04-22 | 范士怀 | Traditional Chinese medicine preparation for treating cervical cancer |
| CN104888149A (en) * | 2015-06-27 | 2015-09-09 | 刘今方 | Drug for treating cervical cancer and preparation method for drug |
| CN104906558A (en) * | 2015-07-08 | 2015-09-16 | 广东天普生化医药股份有限公司 | Application of ulinastatin for preparing cervical cancer treatment medicine and pharmaceutical composition |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111184835A (en) * | 2020-04-09 | 2020-05-22 | 喻小兰 | A pharmaceutical composition prepared from baicalein for treating cervical cancer |
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Application publication date: 20160203 |