CN111165310A - Preparation method of wild pinellia ternata seedling culture nutrient medium - Google Patents
Preparation method of wild pinellia ternata seedling culture nutrient medium Download PDFInfo
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- CN111165310A CN111165310A CN201911143912.3A CN201911143912A CN111165310A CN 111165310 A CN111165310 A CN 111165310A CN 201911143912 A CN201911143912 A CN 201911143912A CN 111165310 A CN111165310 A CN 111165310A
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- nutrient medium
- vermiculite
- carbendazim
- volume ratio
- wild
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
Abstract
The invention discloses a method for preparing a wild pinellia ternate seedling culture nutrient medium, which comprises the following steps of preparing the wild pinellia ternate nutrient medium during sowing: the total thickness of the nutrient medium divided into two layers is 8cm, the thickness of the upper layer is 2cm, the turf is placed, and the volume of the medium is matched with that of the turf: vermiculite: the volume ratio of the perlite is 50: 5: 1, the thickness of the low layer is 6cm, the main components are that the fermented traditional Chinese medicine residue is crushed by 0.1-0.5mm, turfy soil, vermiculite, bean cake and calcium magnesium phosphate fertilizer are added, and the volume ratio of the matrix is traditional Chinese medicine residue powder: turfy soil: bean cake: vermiculite: the calcium magnesium phosphate fertilizer is 4:2:3: 1; the nutrient medium plant growth regulator comprises the following components in percentage by weight: dissolving the plant growth regulator in water, and mixing with the nutrient substrate, wherein IBA is 3ppm, and NAA is 2 ppm; matrix sterilization: mixing with carbendazim at a volume ratio of the nutrient medium to the carbendazim of 1000: 5; and (3) in a strong seedling stage: sterilizing with 50% carbendazim powder once a month, and spraying with the dilution ratio of 1: 700; transplanting the seedlings for the second time, and culturing in a field.
Description
Technical Field
The invention relates to the technical field of traditional Chinese pharmacology, in particular to a method for preparing a wild pinellia ternate seedling culture nutrient medium.
Background
In the prior art, wild pinellia ternate resources are in short supply, and the prior pinellia ternate cultivation technology cannot realize great improvement of yield, and particularly the wild pinellia ternate nutrition matrix configuration in the Huaibei area is mainly embodied in the following points: 1. in the cultivation process, pinellia ternata seed seedlings need to sprout at a lower temperature (8-12 ℃), the most suitable growth temperature is 20-25 ℃, overground parts die due to overhigh temperature, and the seedlings are afraid of high temperature, drought and strong light irradiation; 2. the phenomenon that small tubers grow on tubers easily occurs in the seedling stage, and the cultivation yield of pinellia ternata is seriously influenced; 3. pinellia ternata has shallow root systems belonging to fertile plants, so that the requirement on soil moisture and fertility is high, and fertilization is the key for increasing yield.
In conclusion, how to ensure the strong and strong buds of the tuber of pinellia ternata, no meristem of small tubers, no diseases, uniformity and guarantee the yield of later-period cultivation is a technical problem which needs to be solved urgently at present.
Disclosure of Invention
The technical task of the invention is to provide a method for preparing a wild pinellia ternate seedling culture nutrient medium aiming at the defects, so as to solve the problems in the background technology.
The technical scheme of the invention is realized as follows:
a method for preparing a wild pinellia ternata seedling culture nutrient medium comprises the following steps:
firstly, preparing a wild pinellia ternata nutrient medium during sowing:
1. the total thickness of the nutrient medium is 8cm in two layers.
2. Nutrient matrix stratification and composition:
the thickness of the upper layer is 2cm, the grass carbon is put in, and the volume of the matrix is matched with that of the grass carbon: vermiculite: the volume ratio of the perlite is 50: 5: 1.
the thickness of the lower layer is 6cm, the main components are that the fermented traditional Chinese medicine residue is crushed by 0.1-0.5mm, turfy soil, vermiculite, bean cake (fermented) and calcium magnesium phosphate fertilizer are added, and the volume ratio of the matrix is traditional Chinese medicine residue powder: turfy soil: bean cake: vermiculite: the ratio of calcium to magnesium phosphate is 4:2:3: 1.
3. The nutrient medium plant growth regulator comprises the following components in percentage by weight:
the plant growth regulator is dissolved in water and then mixed with a nutrient substrate, wherein IBA is 3ppm, and NAA is 2 ppm.
4. Matrix sterilization:
mixing with carbendazim at a volume ratio of the nutrient medium to the carbendazim of 1000: 5.
5. And (3) in a strong seedling stage:
the sterilization is carried out once a month by using 50 percent carbendazim powder, and the spraying dilution is 1:700 times.
Secondly, seedling raising of the wild pinellia ternata is carried out for the second time, and the wild pinellia ternata is cultivated in a field.
Compared with the prior art, the invention has the advantages and positive effects that:
the nutrient medium has reasonable nutrient components, has the characteristics of water retention, fertilizer retention, air permeability and the like, and has the advantages that the germination rate of the processed pinellia tuber seeds is 90 percent, the germination time is 15-20 days, the seedlings grow rapidly in the seedling stage, the seedlings are strong, no small tuber is formed, the pinellia tuber is not infected with diseases and the like.
Detailed Description
In order that the above objects, features and advantages of the present invention can be more clearly understood, the present invention will be further described with reference to the following examples. It should be noted that the embodiments and features of the embodiments of the present application may be combined with each other without conflict.
The present invention will be further described with reference to the following specific examples.
In the first embodiment, the preparation method of the wild pinellia ternata seedling culture nutrient medium comprises the following steps of:
step S101: preparing a wild pinellia ternata nutrient medium during sowing:
1. the total thickness of the nutrient medium is 8cm in two layers.
2. Nutrient matrix stratification and composition:
the thickness of the upper layer is 2cm, the grass carbon is put in, and the volume of the matrix is matched with that of the grass carbon: vermiculite: the volume ratio of the perlite is 50: 5: 1;
the thickness of the lower layer is 6cm, the main components are that the fermented traditional Chinese medicine residue is crushed by 0.1-0.5mm, turfy soil, vermiculite, bean cake (fermented) and calcium magnesium phosphate fertilizer are added, and the volume ratio of the matrix is traditional Chinese medicine residue powder: turfy soil: bean cake: vermiculite: the calcium magnesium phosphate fertilizer is 4:2:3: 1;
3. the nutrient medium plant growth regulator comprises the following components in percentage by weight:
dissolving the plant growth regulator in water, and mixing with the nutrient substrate, wherein IBA is 3ppm, and NAA is 2 ppm;
4. matrix sterilization:
mixing with carbendazim at a volume ratio of the nutrient medium to the carbendazim of 1000: 5;
5. and (3) in a strong seedling stage:
sterilizing with 50% carbendazim powder once a month, and spraying with the dilution ratio of 1: 700;
step S102: and (4) carrying out secondary seedling transplanting on the wild pinellia tuber seedlings, and carrying out field cultivation.
For the convenience of understanding the technical solutions of the present invention, the following detailed description will be made on the working principle or the operation mode of the present invention in the practical process.
In the actual application of the method, the device is used,
1. in the pinellia ternate seedling raising process, matrix seedling raising is carried out under a mulberry forest, high temperature and strong light irradiation are avoided, and meanwhile, the higher water retention of the matrix seedling raising provides fundamental guarantee for water supply in the seedling stage.
2. The nutrient medium is added in the seed germination process, and IBA and NAA in a certain proportion are added to inhibit the generation of small tubers.
3. The pinellia ternata seedling raising method has the advantages that the root system is shallow, the pinellia ternata seedling raising method is very suitable for matrix seedling raising, nutrient elements are reasonably proportioned in the seedling raising process to guarantee fertility supply, meanwhile, the matrix seedling raising can effectively inhibit diseases in the seedling stage, and the germination of pinellia ternata seeds and the growth and development of the pinellia ternata in the seedling stage are promoted.
Wherein, IBA: IBA, also called indolebutyric acid, is a substance with hormone activity of artificial synthesis, belong to plant growth regulator (the substance that can control the growth and development of the plant effectively) mainly used for promoting the cuttage of the fruit tree to take root, the adventitious root that causes is many and slender, use for taking root in the tissue culture, indoleacetic acid accommodation is extensive and safe, it is the most main regulator at present.
NAA: naphthylacetic acid, NAA for short, is an organic compound (chemical formula: C10H7CH2CO2H) and is a colorless solid easily soluble in organic solvents. The structure of the plant growth promoter is that the No. 1 position of naphthalene is substituted by carboxymethyl, the plant growth promoter is a plant hormone auxin, is commonly used in commercial rooting powder or rooting agent, is used when plants are propagated by a cuttage method, and can also be used for plant tissue culture.
The present invention can be easily implemented by those skilled in the art from the above detailed description. It should be understood, however, that the intention is not to limit the invention to the particular embodiments described. On the basis of the disclosed embodiments, a person skilled in the art can combine different technical features at will, thereby implementing different technical solutions.
Claims (1)
1. A method for preparing a wild pinellia ternate seedling culture nutrient medium is characterized by comprising the following steps:
s101: preparing a wild pinellia ternata nutrient medium during sowing: the total thickness of the nutrient medium divided into two layers is 8cm, the thickness of the upper layer is 2cm, the turf is placed, and the volume of the medium is matched with that of the turf: vermiculite: the volume ratio of the perlite is 50: 5: 1, the thickness of the low layer is 6cm, the main components are that the fermented traditional Chinese medicine residue is crushed by 0.1-0.5mm, turfy soil, vermiculite, bean cake (fermented) and calcium magnesium phosphate fertilizer are added, and the volume ratio of the matrix is traditional Chinese medicine residue powder: turfy soil: bean cake: vermiculite: the calcium magnesium phosphate fertilizer is 4:2:3: 1;
the nutrient medium plant growth regulator comprises the following components in percentage by weight: dissolving the plant growth regulator in water, and mixing with the nutrient substrate, wherein IBA is 3ppm, and NAA is 2 ppm;
matrix sterilization: mixing with carbendazim at a volume ratio of the nutrient medium to the carbendazim of 1000: 5;
and (3) in a strong seedling stage: sterilizing with 50% carbendazim powder once a month, and spraying with the dilution ratio of 1: 700;
s102: and (4) carrying out secondary seedling transplanting on the wild pinellia tuber seedlings, and carrying out field cultivation.
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| CN201911143912.3A CN111165310A (en) | 2019-11-20 | 2019-11-20 | Preparation method of wild pinellia ternata seedling culture nutrient medium |
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| CN201911143912.3A CN111165310A (en) | 2019-11-20 | 2019-11-20 | Preparation method of wild pinellia ternata seedling culture nutrient medium |
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| CN111165310A true CN111165310A (en) | 2020-05-19 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113207591A (en) * | 2021-05-12 | 2021-08-06 | 贵州大学 | Method for cultivating seedlings by pinellia ternata bulbels |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107466853A (en) * | 2017-08-25 | 2017-12-15 | 衢州康源生物科技有限公司 | A kind of breeding method for the tuber of pinellia seedling stem that hereditary variation is small, survival rate is high |
| CN108124732A (en) * | 2017-12-01 | 2018-06-08 | 亳州学院 | Dabie Mountain wild bletilla striata nutrition sowing media configuration method |
| CN109565997A (en) * | 2018-11-28 | 2019-04-05 | 绩溪县徽菜宝生物科技有限公司 | A kind of implantation methods of the tuber of pinellia |
| CN110036851A (en) * | 2019-04-15 | 2019-07-23 | 重庆市药物种植研究所 | A kind of tuber of pinellia floods and droughts segmented seed growing method |
-
2019
- 2019-11-20 CN CN201911143912.3A patent/CN111165310A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107466853A (en) * | 2017-08-25 | 2017-12-15 | 衢州康源生物科技有限公司 | A kind of breeding method for the tuber of pinellia seedling stem that hereditary variation is small, survival rate is high |
| CN108124732A (en) * | 2017-12-01 | 2018-06-08 | 亳州学院 | Dabie Mountain wild bletilla striata nutrition sowing media configuration method |
| CN109565997A (en) * | 2018-11-28 | 2019-04-05 | 绩溪县徽菜宝生物科技有限公司 | A kind of implantation methods of the tuber of pinellia |
| CN110036851A (en) * | 2019-04-15 | 2019-07-23 | 重庆市药物种植研究所 | A kind of tuber of pinellia floods and droughts segmented seed growing method |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113207591A (en) * | 2021-05-12 | 2021-08-06 | 贵州大学 | Method for cultivating seedlings by pinellia ternata bulbels |
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Application publication date: 20200519 |
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