CN110073761A - A kind of method of bletilla striata seeds direct sowing and seedling - Google Patents
A kind of method of bletilla striata seeds direct sowing and seedling Download PDFInfo
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- CN110073761A CN110073761A CN201811265835.4A CN201811265835A CN110073761A CN 110073761 A CN110073761 A CN 110073761A CN 201811265835 A CN201811265835 A CN 201811265835A CN 110073761 A CN110073761 A CN 110073761A
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- 241001313857 Bletilla striata Species 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims abstract description 25
- 238000009331 sowing Methods 0.000 title claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 34
- 239000003337 fertilizer Substances 0.000 claims abstract description 10
- 241000196324 Embryophyta Species 0.000 claims abstract description 6
- 238000010899 nucleation Methods 0.000 claims abstract description 6
- 239000000126 substance Substances 0.000 claims abstract description 5
- 239000000243 solution Substances 0.000 claims description 22
- 235000015097 nutrients Nutrition 0.000 claims description 16
- 239000004576 sand Substances 0.000 claims description 15
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 14
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 14
- 239000006013 carbendazim Substances 0.000 claims description 14
- 239000008399 tap water Substances 0.000 claims description 14
- 235000020679 tap water Nutrition 0.000 claims description 14
- 241000209094 Oryza Species 0.000 claims description 12
- 235000007164 Oryza sativa Nutrition 0.000 claims description 12
- 235000009566 rice Nutrition 0.000 claims description 12
- 239000010902 straw Substances 0.000 claims description 12
- 239000002775 capsule Substances 0.000 claims description 10
- 239000005556 hormone Substances 0.000 claims description 10
- 229940088597 hormone Drugs 0.000 claims description 10
- 239000007864 aqueous solution Substances 0.000 claims description 9
- 239000003795 chemical substances by application Substances 0.000 claims description 9
- 238000003860 storage Methods 0.000 claims description 9
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 8
- 238000002791 soaking Methods 0.000 claims description 8
- 238000005406 washing Methods 0.000 claims description 8
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 claims description 5
- 239000012530 fluid Substances 0.000 claims description 5
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 claims description 5
- 229960001669 kinetin Drugs 0.000 claims description 5
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims description 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 4
- 239000000908 ammonium hydroxide Substances 0.000 claims description 4
- 238000001816 cooling Methods 0.000 claims description 4
- 239000003814 drug Substances 0.000 claims description 4
- 230000007062 hydrolysis Effects 0.000 claims description 4
- 238000006460 hydrolysis reaction Methods 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 230000010355 oscillation Effects 0.000 claims description 4
- 239000010451 perlite Substances 0.000 claims description 4
- 235000019362 perlite Nutrition 0.000 claims description 4
- 238000003672 processing method Methods 0.000 claims description 4
- 239000002689 soil Substances 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 3
- 239000007921 spray Substances 0.000 claims description 3
- 239000005708 Sodium hypochlorite Substances 0.000 claims 1
- 239000000645 desinfectant Substances 0.000 claims 1
- GPXLRLUVLMHHIK-UHFFFAOYSA-N forchlorfenuron Chemical compound C1=NC(Cl)=CC(NC(=O)NC=2C=CC=CC=2)=C1 GPXLRLUVLMHHIK-UHFFFAOYSA-N 0.000 claims 1
- 239000007788 liquid Substances 0.000 claims 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 claims 1
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 3
- 238000005516 engineering process Methods 0.000 abstract description 2
- 238000005507 spraying Methods 0.000 description 6
- 238000009395 breeding Methods 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 4
- 230000035784 germination Effects 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 3
- 238000003306 harvesting Methods 0.000 description 3
- QWPPOHNGKGFGJK-UHFFFAOYSA-N hypochlorous acid Chemical compound ClO QWPPOHNGKGFGJK-UHFFFAOYSA-N 0.000 description 3
- 229910052708 sodium Inorganic materials 0.000 description 3
- 239000011734 sodium Substances 0.000 description 3
- 241001313855 Bletilla Species 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000003475 lamination Methods 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 229920006395 saturated elastomer Polymers 0.000 description 2
- 230000007226 seed germination Effects 0.000 description 2
- 206010054949 Metaplasia Diseases 0.000 description 1
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000015689 metaplastic ossification Effects 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- 229940072033 potash Drugs 0.000 description 1
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 description 1
- 235000015320 potassium carbonate Nutrition 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000027272 reproductive process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000012090 tissue culture technique Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 125000000391 vinyl group Chemical group [H]C([*])=C([H])[H] 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/25—Dry fruit hulls or husks, e.g. chaff or coir
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Fertilizers (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to field of plant growing technology, disclose a kind of method of bletilla striata seeds direct sowing and seedling comprising following steps: step A seed collection, step B seed chemicals treatment, step C type of seeding, step D nursery water and fertilizer management.The method of the present invention planting percent is high, and low in cost, easy to operate, reproduction speed is fast, is suitble to large-scale production.
Description
Technical field
The invention belongs to field of plant growing technology, and in particular to a kind of method of bletilla striata seeds direct sowing and seedling.
Background technique
The bletilla striata Bletilla striata (Thunb.) Reiehb.f., orchid family bletilla striata category (Bletilla) perennial root
Herbaceous plant, dry tuber are used as medicine, and have astringing to arrest bleeding, detumescence and promoting granulation and other effects.Modern pharmacology experimental study shows the bletilla striata also
With pharmacological actions such as antitumor, antibacterials.In addition, the bletilla striata is also widely used in afforestation, food processing and the raw material of industry.
Bletilla striata raw materials market demand rapid increase in recent years, wild bletilla striata are excavated by madness, cause its wild resource storage anxious
It reduces sharply less, it is endangered, one of the Wild Medicinal of focused protection is classified as by country.In order to protect bletilla striata wild resource, protect
Hinder bletilla striata market supply, the Wild of the bletilla striata is imperative.
Seedling breeding is the important link of plant Wild, the side such as generallys use sowing, cuttage, divides root and tissue culture
Formula.Bletilla striata seeds are extremely more, and each scape can be fruit pod 3-8 solid, and each fruit pod 2000-10000 containing seed, but its seed
It is small, it is in powdered, and seed endosperm is extremely undeveloped, only 2-3 albuminous cell is scattered naturally and fosters planting percent with conventional manual
It is extremely low.Current bletilla striata farmers' mostly uses Split-Root Nutrient Solution Method to breed, but its breeding coefficient is 2-3 times, and therefore, bletilla striata seedling breeding becomes
The bottleneck of bletilla striata Wild large-scale production.Using tissue culture technique, the rescue of bletilla striata idiosome is carried out, is current research
Hot spot and the effective propagation method of the bletilla striata, correlative study have reported success.However, being passed through using bletilla striata seeds
Sterile culture nursery, the period is long, at high cost, cumbersome.
Summary of the invention
The purpose of the present invention is to provide a kind of methods of bletilla striata seeds direct sowing and seedling, and this method planting percent is high, at low cost
Honest and clean, easy to operate, reproduction speed is fast, is suitble to large-scale production.
The present invention is achieved by the following technical solution:
A kind of method of bletilla striata seeds direct sowing and seedling comprising following steps: step A seed collection, at step B seed medicament
Reason, step C type of seeding, step D nursery water and fertilizer management.
Specifically, described method includes following steps:
Step A seed collection: when seed hull switchs to withered and yellow, in fine day, band scape harvests together in the afternoon, and ventilating and cooling place is dried in the air
Dry 5-12 hours, then seed soaked in cold water 7 days, it was primary to change water daily, dried the surface of the seed moisture after seed soaking, sand at low temperature
Hiding, by sand: seed 3:1 is carried out, and storage period is within 8 months；
Step B seed chemicals treatment:
(1) Seed sterilization: bletilla striata capsule prepared by step A submerges 30s with 75% alcohol, through 5% hypochlorous acid after aseptic water washing
Sodium solution sterilizes 10min, is during which kept stirred, aseptic water washing 3-4 times, aseptic filter paper blots bletilla striata capsule surface moisture；(2)
Hormone agents: 1/2 MS fluid nutrient medium+5mg/L BA+ 0.5mg/ KT kinetin+5mg/LCPPU+1mg/LNAA are prepared,
Adjust pH 5.6-5.8；
(3) processing method: being cut off bletilla striata capsule top with scissors, seed is uniformly shaken off in the triangular flask that hormone agents are housed
In, it under dark condition, is placed on shaking table and shakes 48-120 hours, shaking table oscillation frequency is 100 revs/min；Then attached bag will be planted
It is wrapped in two-layer nonwoven bag, originally water droplet is washed 120-180 minutes, 2%(mass fraction) carbendazim aqueous solution soaking 30 minutes,
Jing Fengchu dries in the air to the surface of the seed without obvious ponding, spare；
Step C type of seeding:
(1) medium of seedling bed is prepared, according to moss: coco bran: perlite: turfy soil: and rice straw hydrolyzate (1-3): (5-
6): (10-12): (15-20): (5-6) mass ratio mixing, add the carbendazim of one thousandth mass parts, mix thoroughly to get；
(2) it is sowed after the fine sand of laying depth 10cm above medium of seedling bed；
Step D nursery water and fertilizer management: (1) after planting until emergence, more bacterium of seedbed auto spraying tap water and 3% mass fraction
Lingshui Spring solution, wherein tap water, 2 hours/time, 10-20ml/ 10m2；Carbendazim aqueous solution, 5 days/time, 50ml/ times
10m2；(2) after emerging, seedbed auto spraying tap water and nutrient solution, wherein hour/time tap water 4-5,20-40ml/ times
10m2；Nutrient solution be 1/4 Hoagland nutrient solution, 5 days/time, 50ml/ 10m2。
The rice straw hydrolyzate is prepared as follows and obtains: rice straw put into pulverizer and is crushed,
50 meshes are crossed, the concentration for then adding double weight is the hydrochloric acid of 5M, 200rpm stirring hydrolysis 6 hours, finally adds ammonium hydroxide, adjusts
Save solution pH be 6.9-7.1 to get.
The beneficial effect that the present invention obtains specifically includes that
The present invention is using sand storage after cold water pretreatment, since sand storage vernalization is a slow water absorption course, while being also one
The process for slowly converting and germinateing, Seed sprouting is related to seed moisture proportion, and only seed-bearing moisture content is close to or up to full
Just start to sprout when with moisture content, and reaches moisture content phase of the time required for saturated aqueous rate when starting lamination with seed
It closes, moisture content is lower before lamination, absorbs water slower, and the time for reaching saturated aqueous rate is longer, is suitable for that can also get over the time of vernalization
It is long；Inventor is found through experiments that, carries out seed soaked in cold water 7 days before sand storage, and it is primary to change water daily, such pretreatment
Afterwards, the bletilla striata seeds that can make just reach saturation state quickly, shorten germination time, and the pretreatment can increase field germination
Rate.
For plant vegetative propagation field, hormone kind and concentration play the role of highly significant to the growth and development of seed,
The application by many experiments find, 1/2 MS fluid nutrient medium+5mg/L BA+ 0.5mg/ KT kinetin+5mg/LCPPU+
1mg/LNAA adjusts hormone agents treated the seed of pH 5.6-5.8, can greatly improve germination rate and survival rate, bletilla seed
The speed of growth and germination rate of son significantly improve.
The present invention is by preparing specific medicament, matrix and compatibility carbendazim, improving the bud ratio of the bletilla striata and surviving
Rate, avoid tissue cultures mode low reproduction rate and it is at high cost the defects of；Operation is simple and feasible for present invention process, is suitble to scale
Metaplasia produces；By breeding above, 98% or more, whole pollution rate is and entire less than 0.1% for the survival rate of obtained bletilla striata seedling
Reproductive process cultivation cycle is less than 120 days, easy to operate without transfer, at low cost, is reduced than traditional plate method
55%.
Specific embodiment
It will should not be construed as below using specific embodiment come the present invention will be further explained to this hair
The limitation of bright initiative spirit.
Embodiment 1
A kind of method of bletilla striata seeds direct sowing and seedling comprising following steps:
Step A seed collection:: after the purple green of bletilla striata seeds shells is thin out, complete stool sprays foliar fertilizer (when section's biotechnology Shanghai have
The multielement foliage fertilizer of limit company production) and potash fertilizer (mass ratio of potassium dihydrogen phosphate and water is 1 to 1000)；To seed hull
When switching to withered and yellow, in fine day, band scape harvests together in the afternoon, and ventilating and cooling place dries 5-12 hours, then seed soaked in cold water 7
It, it is primary to change water daily, dries the surface of the seed moisture after seed soaking, low temperature sand storage, and by sand: seed 3:1 is carried out, and storage period exists
Within 8 months；
Step B seed chemicals treatment:
(1) Seed sterilization: bletilla striata capsule prepared by step A submerges 30s with 75% alcohol, through 5% hypochlorous acid after aseptic water washing
Sodium solution sterilizes 10min, is during which kept stirred, aseptic water washing 3-4 times, aseptic filter paper blots bletilla striata capsule surface moisture；(2)
Hormone agents: 1/2 MS fluid nutrient medium+5mg/L BA+ 0.5mg/ KT kinetin+5mg/LCPPU+1mg/LNAA are prepared,
Adjust pH 5.6-5.8；
(3) processing method: being cut off bletilla striata capsule top with scissors, seed is uniformly shaken off in the triangular flask that hormone agents are housed
In, it under dark condition, is placed on shaking table and shakes 48 hours, shaking table oscillation frequency is 100 revs/min；Then seed is wrapped in
In two-layer nonwoven bag, originally water droplet is washed 120 minutes, and 2% carbendazim aqueous solution soaking 30 minutes, Jing Fengchu dries in the air to the surface of the seed
It is spare without obvious ponding；
Step C type of seeding:
(1) medium of seedling bed is prepared, according to moss: coco bran: perlite: turfy soil: and rice straw hydrolyzate 1:5:10:15:5
Mass ratio mixing, add the carbendazim of one thousandth mass parts, mix thoroughly to get；
(2) it is sowed after the fine sand of laying depth 10cm above medium of seedling bed, seedbed is set in heliogreenhouse or vinyl house；It broadcasts
The kind time is autumn seed collection current year or spring next year；
Step D nursery water and fertilizer management: (1) after planting until emergence, more bacterium of seedbed auto spraying tap water and 3% mass fraction
Lingshui Spring solution, wherein tap water, 2 hours/time, 10-20ml/ 10m2；Carbendazim aqueous solution, 5 days/time, 50ml/ times
10m2；(2) after emerging, seedbed auto spraying tap water and nutrient solution, wherein hour/time tap water 4-5,20-40ml/ times
10m2；Nutrient solution be 1/4 Hoagland nutrient solution, 5 days/time, 50ml/ 10m2。
The rice straw hydrolyzate is prepared as follows and obtains: rice straw put into pulverizer and is crushed,
50 meshes are crossed, the concentration for then adding double weight is the hydrochloric acid of 5M, 200rpm stirring hydrolysis 6 hours, finally adds ammonium hydroxide, adjusts
Save solution pH be 6.9-7.1 to get.
By above method, seed germination rate reaches 96.4%, and 98%, whole pollution rate is less than the survival rate of bletilla striata seedling
0.1%, and it is entire at low cost, 55% is reduced than traditional plate method.
Embodiment 2
A kind of method of bletilla striata seeds direct sowing and seedling comprising following steps:
Step A seed collection: when seed hull switchs to withered and yellow, in fine day, band scape harvests together in the afternoon, and ventilating and cooling place is dried in the air
Dry 5-12 hours, then seed soaked in cold water 7 days, it was primary to change water daily, dried the surface of the seed moisture after seed soaking, sand at low temperature
Hiding, by sand: seed 3:1 is carried out, and storage period is within 8 months；
Step B seed chemicals treatment:
(1) Seed sterilization: bletilla striata capsule prepared by step A submerges 30s with 75% alcohol, through 5% hypochlorous acid after aseptic water washing
Sodium solution sterilizes 10min, is during which kept stirred, aseptic water washing 3-4 times, aseptic filter paper blots bletilla striata capsule surface moisture；(2)
Hormone agents: 1/2 MS fluid nutrient medium+5mg/L BA+ 0.5mg/ KT kinetin+5mg/LCPPU+1mg/LNAA are prepared,
Adjust pH 5.6-5.8；
(3) processing method: being cut off bletilla striata capsule top with scissors, seed is uniformly shaken off in the triangular flask that hormone agents are housed
In, it under dark condition, is placed on shaking table and shakes 120 hours, shaking table oscillation frequency is 100 revs/min；Then seed is wrapped in
In two-layer nonwoven bag, originally water droplet is washed 180 minutes, and 2% carbendazim aqueous solution soaking 30 minutes, Jing Fengchu dries in the air to the surface of the seed
It is spare without obvious ponding；
Step C type of seeding:
(1) medium of seedling bed is prepared, according to moss: coco bran: perlite: turfy soil: and rice straw hydrolyzate is according to 3:6:12:
The mass ratio of 20:6 mixes, and adds the carbendazim of one thousandth mass parts, mix thoroughly to get；
(2) it is sowed after the fine sand of laying depth 10cm above medium of seedling bed；
Step D nursery water and fertilizer management: (1) after planting until emergence, more bacterium of seedbed auto spraying tap water and 3% mass fraction
Lingshui Spring solution, wherein tap water, 2 hours/time, 10-20ml/ 10m2；Carbendazim aqueous solution, 5 days/time, 50ml/ times
10m2；(2) after emerging, seedbed auto spraying tap water and nutrient solution, wherein hour/time tap water 4-5,20-40ml/ times
10m2；Nutrient solution be 1/4 Hoagland nutrient solution, 5 days/time, 50ml/ 10m2。
The rice straw hydrolyzate is prepared as follows and obtains: rice straw put into pulverizer and is crushed,
50 meshes are crossed, the concentration for then adding double weight is the hydrochloric acid of 5M, 200rpm stirring hydrolysis 6 hours, finally adds ammonium hydroxide, adjusts
Save solution pH be 6.9-7.1 to get.
By above method, seed germination rate reaches 97.5%, and 99%, entirety pollutes the survival rate of obtained bletilla striata seedling
Rate is at low cost less than 0.1%, reduces 55% than traditional plate method.
The above list is only a few specific embodiments of the present invention for finally, it should also be noted that.Obviously, this hair
Bright to be not limited to above embodiments, acceptable there are many deformations.Those skilled in the art can be from present disclosure
All deformations for directly exporting or associating, are considered as protection scope of the present invention.
Claims (3)
1. a kind of method of bletilla striata seeds direct sowing and seedling comprising following steps: step A seed collection, at step B seed medicament
Reason, step C type of seeding, step D nursery water and fertilizer management.
2. the method according to claim 1, wherein described method includes following steps:
Step A seed collection:
Seed is harvested, dries 5-12 hour in ventilating and cooling place, then soaked in cold water 7 days, it is primary to change water daily, will plant after seed soaking
Sub- surface moisture dries, low temperature sand storage, by sand: seed=3:1 mass ratio carries out；
Step B seed chemicals treatment:
(1) Seed sterilization: seed prepared by step A submerges 30s with 75% alcohol, molten through 5% sodium hypochlorite after aseptic water washing
Liquid disinfectant 10min, is during which kept stirred, and aseptic water washing 3-4 blots surface moisture all over rear aseptic filter paper；
(2) hormone agents are prepared: 1/2 MS fluid nutrient medium+5mg/L BA+0.5mg/ KT kinetin+5mg/L CPPU+
1mg/L NAA adjusts pH 5.6-5.8；
(3) processing method: being cut off bletilla striata capsule top with scissors, seed is uniformly shaken off in the triangular flask that hormone agents are housed
In, it under dark condition, is placed on shaking table and shakes 48-120 hours, shaking table oscillation frequency is 100 revs/min；Then attached bag will be planted
It is wrapped in two-layer nonwoven bag, originally water droplet is washed 120-180 minutes, and 2% carbendazim aqueous solution soaking 30 minutes is dried in the air to kind of a sublist
Face is spare without obvious ponding；
Step C sowing:
(1) medium of seedling bed is prepared, according to moss: coco bran: perlite: turfy soil: rice straw hydrolyzate=(1-3): (5-6):
(10-12): (15-20): (5-6) mass ratio mixing, add the carbendazim of one thousandth mass parts, mix thoroughly to get；
(2) it is sowed after the fine sand of laying depth 10cm above medium of seedling bed；
Step D nursery water and fertilizer management:
(1) after planting until emergence, seedbed spray the carbendazim aqueous solution of tap water and 3% automatically, wherein tap water, 2 hours/
It is secondary, 10-20ml/ 10m2；Carbendazim aqueous solution, 5 days/time, 50ml/ 10m2；(2) after emerging, seedbed sprays certainly automatically
Water and nutrient solution.
3. according to the method described in claim 2, it is characterized in that, the rice straw hydrolyzate be prepared as follows and
: rice straw to be put into pulverizer and is crushed, 50 meshes are crossed, the concentration for then adding double weight is the hydrochloric acid of 5M,
200rpm stirring hydrolysis 6 hours, finally add ammonium hydroxide, adjust solution pH be 6.9-7.1 to get.
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| CN111567179A (en) * | 2020-06-03 | 2020-08-25 | 重庆市药物种植研究所 | Method for promoting direct seeding germination of bletilla striata seeds and germination agent |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106068790A (en) * | 2016-07-19 | 2016-11-09 | 江苏省中国科学院植物研究所 | A kind of method of bletilla striata seeds direct sowing and seedling |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106068790A (en) * | 2016-07-19 | 2016-11-09 | 江苏省中国科学院植物研究所 | A kind of method of bletilla striata seeds direct sowing and seedling |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111567179A (en) * | 2020-06-03 | 2020-08-25 | 重庆市药物种植研究所 | Method for promoting direct seeding germination of bletilla striata seeds and germination agent |
| CN111567179B (en) * | 2020-06-03 | 2022-03-11 | 重庆市药物种植研究所 | Method for promoting direct seeding germination of bletilla striata seeds and germination agent |
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Application publication date: 20190802 |