CN110934766B - Wrinkle-removing essence containing recombinant collagen - Google Patents
Wrinkle-removing essence containing recombinant collagen Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/84—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds obtained by reactions otherwise than those involving only carbon-carbon unsaturated bonds
- A61K8/88—Polyamides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
Abstract
The invention discloses a wrinkle-removing essence containing recombinant collagen, belonging to the field of cosmetics. The wrinkle-removing essence comprises the following components in percentage by mass: 0.1-1% of recombinant collagen, 10-30% of glycerol, 1-5% of 1, 2-hexanediol, 0.1-0.5% of sodium hyaluronate, 0.1-0.5% of polyglutamic acid, 0.03-2% of acetyl hexapeptide-80.05, 0.03-2% of elastin, 0.01-1.5% of fibronectin and the balance of water. The invention adopts the recombinant collagen from microbial fermentation to be compounded with other raw materials, and the problems of precipitation, color change, precipitation and the like can not occur. The wrinkle-removing essence disclosed by the invention is prepared by compounding the recombinant collagen with sodium hyaluronate, sodium polyglutamate and the like, and can better exert the effects of compacting and removing wrinkles of the recombinant collagen while keeping moisture and locking water.
Description
Technical Field
The invention belongs to the technical field of cosmetic preparations, and relates to wrinkle-removing essence containing recombinant collagen.
Background
With the improvement of living standard, the requirements of people on the quality of life are higher and higher, and the facial appearance is more emphasized. Wrinkles are the most obvious and important feature of skin aging. Wrinkles in humans typically begin to appear around the age of 20 and gradually increase and deepen with age. With the age, the epidermis tissue of the skin becomes dry and thin, collagen and elastin gradually run off, and the mesh structure of the fine sieve formed by interweaving the collagen fibers and the elastin fibers is damaged, so that wrinkles with different thicknesses can appear. A person typically begins collagen loss at age 20, enters a peak period of loss at age 25, and has less than half the collagen content at age 40 than at age 18. Therefore, it is very difficult to supplement collagen required by human body. Sufficient collagen is supplemented to achieve the effects of tightening skin and removing wrinkles. At present, most of the collagen used in wrinkle-removing products on the market is extracted from animal bones or skin, for example, marine collagen extracted from deep-sea fish. However, because of animal origin, the collagen still has biological safety hazards, the wide application of the collagen in various products such as cosmetics, foods, medicines and the like is greatly limited, and the wrinkle removing effect is still not ideal.
Disclosure of Invention
The invention aims to provide a wrinkle-removing essence containing recombinant collagen. The wrinkle-removing essence adopts recombinant collagen with good skin affinity and histocompatibility as raw materials, and has effects of moisturizing, retaining water, tightening and removing wrinkles.
The technical scheme for realizing the purpose of the invention is as follows:
the wrinkle-removing essence containing the recombinant collagen comprises the following components in percentage by mass: 0.1-1% of recombinant collagen, 10-30% of glycerol, 1-5% of 1, 2-hexanediol, 0.1-0.5% of sodium hyaluronate, 0.1-0.5% of polyglutamic acid, 0.03-2% of acetyl hexapeptide-80.05, 0.03-2% of elastin, 0.01-1.5% of fibronectin and the balance of water.
Preferably, the wrinkle-removing essence comprises the following components in percentage by mass: 0.8% of recombinant collagen, 30% of glycerol, 2.5% of 1, 2-hexanediol, 0.5% of sodium hyaluronate, 0.5% of polyglutamic acid, 0.06% of acetyl hexapeptide-80.05%, 0.06% of elastin, 0.03% of fibronectin and the balance of water.
In the wrinkle-removing essence disclosed by the invention, glycerol is used as a humectant, 1, 2-hexanediol is used as a humectant, recombinant collagen is used as a skin conditioner, sodium hyaluronate is used as a humectant, polyglutamic acid is used as a humectant, acetyl hexapeptide-8 is used as a humectant, elastin is used as a humectant, and fibronectin is used as a humectant.
As a preferable scheme of the invention, the recombinant collagen is produced by fermentation of Pichia pastoris with the preservation number of CGMCC No. 5021. The recombinant collagen adopted by the invention takes a human collagen gene as a template, is optimized and modified, is introduced into pichia pastoris, and is obtained by fermentation induction, separation and purification. The recombinant collagen is highly consistent with human collagen, and has good skin affinity and tissue compatibility.
The invention also provides a preparation method of the wrinkle-removing essence containing the recombinant collagen, which comprises the following steps:
according to the proportion, sequentially adding sodium hyaluronate, polyglutamic acid, recombinant collagen, elastin and fibronectin into glycerol, stirring and mixing uniformly, then adding 1, 2-hexanediol, adding water to 100%, stirring uniformly until completely dissolving, and obtaining the wrinkle-removing essence containing the recombinant collagen.
Compared with the prior art, the invention has the following advantages:
the invention adopts the molecular weight determination of the recombinant collagen from microbial fermentation, is the single-component collagen and has no peculiar smell. It has good water solubility, maximum solubility of 20%, good transdermal property, and easy absorption. In addition, the recombinant collagen is derived from microbial fermentation, has good performance when being compounded with other raw materials, and can not generate the problems of precipitation, color change, precipitation and the like. The wrinkle-removing essence disclosed by the invention is prepared by compounding the recombinant collagen with sodium hyaluronate, polyglutamic acid and the like, and can better exert the effects of compacting and removing wrinkles of the recombinant collagen while keeping moisture and locking water.
Drawings
Fig. 1 is a graph showing wrinkle improvement rates in T group, S group, and a group after 8 weeks of use.
Fig. 2 is a graph of pore improvement rates in T, S and a groups after 8 weeks of use.
Fig. 3 is a graph showing the water improvement rate of T, S and a group after 8 weeks of use.
Fig. 4 is a graph of texture improvement using T, S and a groups after 8 weeks.
Fig. 5 is an image acquisition analysis chart (a) and a real object chart (B) of the left canthus of a group a of test persons 1 before use (W0) and after use for 8 weeks (W8).
Fig. 6 is a pictorial representation of the left canthus of group a human subjects 2 before use (W0) and after 8 weeks of use (W8).
Detailed Description
The present invention will be described in more detail with reference to the following examples and drawings, which are given by way of illustration only and are not intended to limit the scope of the present invention.
Example 1
The wrinkle-removing essence containing the recombinant collagen comprises the following components in percentage by mass: 0.8% of recombinant collagen, 30% of glycerol, 2.5% of 1, 2-hexanediol, 0.5% of sodium hyaluronate, 0.5% of polyglutamic acid, 0.06% of acetyl hexapeptide-80.05%, 0.06% of elastin, 0.03% of fibronectin and the balance of water. Is prepared by the following steps: according to the formula proportion, sequentially adding sodium hyaluronate, polyglutamic acid, recombinant collagen, elastin and fibronectin into glycerol, stirring until the mixture is uniformly mixed, then adding 1, 2-hexanediol, adding water to 100%, and stirring until the mixture is completely dissolved to obtain the wrinkle-removing essence containing the recombinant collagen.
In the embodiment, the recombinant collagen is produced by fermenting Pichia pastoris with the preservation number of CGMCC No.5021, and other raw materials are conventional products sold in the market.
In this embodiment, the raw materials may be flexibly combined within a given mixture ratio range, which is not always enumerated herein.
Example 2
1. Experimental products:
and (3) frosting: 68.65% of water, 0.2% of carbomer, 0.2% of triethanolamine, 0.1% of xanthan gum, 4% of glycerol, 3% of butanediol, 20.5% of bioglycan gum, 1% of cetostearyl alcohol, 0.8% of C20-22 alcohol phosphate (and) C20-22 alcohol, 0.5% of glyceryl stearate, 3% of caprylic/capric glycerides, 3% of polydimethylsiloxane, 4.5% of shea butter (Butyrrhiza PARKII), 0.15% of methylparaben, 0.4% of caprylyl hydroxamic acid and 0.5% of 1, 2-hexanediol.
The wrinkle-removing essence containing the recombinant collagen comprises the following components in percentage by mass: 0.8% of recombinant collagen, 30% of glycerol, 2.5% of 1, 2-hexanediol, 0.5% of sodium hyaluronate, 0.5% of polyglutamic acid, 0.06% of acetyl hexapeptide-80.05%, 0.06% of elastin, 0.03% of fibronectin and the balance of water.
The wrinkle-removing essence free of recombinant collagen comprises the following components in percentage by mass: 30% of glycerol, 2.5% of 1, 2-hexanediol, 0.5% of sodium hyaluronate, 0.5% of polyglutamic acid, 0. 80.05% of acetyl hexapeptide, 0.06% of elastin, 0.03% of fibronectin and the balance of water.
2. Experiment grouping
The experiment is divided into a group T, a group S and a group A, the same batch of cream with the same formula is used as a substrate, the authenticity of the experiment is ensured, the group T is a placebo group, 10% of purified water is added, the purified water is homogenized and stirred uniformly, the group A is added with 10% of collagen tightening peptide stock solution, the collagen tightening peptide without recombinant human collagen is added, the group S is the collagen tightening peptide without recombinant human collagen, and the collagen tightening peptide is added.
Group T (placebo group): frost + 10% purified water;
group S (control group): cream + 10% wrinkle-removing essence without recombinant collagen;
group a (experimental group): cream + 10% wrinkle-removing essence containing recombinant collagen.
3. The test purpose is as follows: open 8 week test, changes in moisturization, wrinkles, pores, texture efficacy after 2 weeks, 4 weeks, 8 weeks of product use were assessed by instrumental testing.
4. The subject claims:
4.1 inclusion criteria
(1) Chinese female;
(2) age 22-55 years;
(3) dry or semi-dry skin;
(4) those with fine lines or wrinkles on the face;
(5) the subject should be insensitive to common cosmetics;
(6) subjects did not participate in other clinical studies for the last 3 months.
4.2 exclusion criteria
(1) Antihistamines administered in the near week or immunosuppressants administered in the near month;
(2) any anti-inflammatory drug is used on the tested part in the last 2 months;
(3) insulin-dependent diabetic patients;
(4) patients suffering from respiratory diseases undergoing treatment;
(5) lactating or pregnant women;
(6) people with allergic constitution or allergic dermatitis have a history of skin diseases or diseases;
(7) a population undergoing dermatological treatment, or a subject taking within one month hydroxy-acids, whitening and anti-aging drugs;
(8) the skin characterization that the test region has large-area birthmarks, scratch marks, white spots, pigmented nevi, pimples and scars and the like which influence the experiment is carried out, and in addition, the responsible person of the experiment judges that the skin characterization is not suitable for the crowd as the experimental object.
4.3 limitations on items
(1) During the test period, the treatment of the influence test on the tested skin is forbidden;
(2) during the test period, retesting with other skin care products and other products affecting the test was prohibited;
(3) during the test period, daily products such as bath foam, soap, shampoo and the like are consistent with those used before the test is started, and replacement is prohibited.
4.4 target number of experiments: eligible subjects were enrolled for 17, ensuring a final completion of 10.
5. Testing an instrument:
(1) skin moisture tester (mileure intelligent skin moisture tester)
(2) Antera3D skin tester (Ireland)
(3) Canon E600 camera
(4) Balance: sensory quantity 0.01g (Mettler)
And (3) testing environment: the temperature is 25 +/-1 ℃, the humidity is 50% +/-5%, and the light rays are ensured to be unchanged every time.
6. The experimental process comprises the following steps:
(1) at the first visit, subjects were instructed to perform the trial and signed an informed consent.
(2) And (4) screening the test subjects according to the test requirements, screening 17 subjects in the group, and finally ensuring more than 10 subjects.
(3) Screening qualified subjects can not use any cosmetics in the morning, after the faces are cleaned with clear water, the faces are rested for 30 minutes in a constant temperature and humidity room, and after 30 minutes, the faces are tested.
(4) After the test is finished, the subject is used for instruction, after the subject listens for the instruction, the product is dispensed, the subject uses the test sample at home for 8 weeks, during the period, the subject visits back to the appointed place after 2 weeks, 4 weeks and 8 weeks of the continuous product according to the instruction of the test, and the face test is carried out according to the step (3). The amount of product used was confirmed at the beginning and end of the test.
7. And (3) observing test items:
subjects were tested 4 times 2 weeks, 4 weeks, 8 weeks after use prior to use:
(1) skin moisture was measured by using a mileure test 3 times and averaged, and the test position was near the temple on the right face of the volunteer.
And (4) judging a result: after the product is used, the moisture content in the test area is obviously increased, and the tested sample has a moisturizing effect.
(2) Skin wrinkles: wrinkle analysis with antera3D combined with canon camera image acquisition.
And (4) judging a result: the picture was taken with antera3D and wrinkles were analyzed.
(3) Skin texture: texture canon camera image acquisition was analyzed with antea 3D.
And (4) judging a result: the texture was analyzed by photographing with antera 3D.
8. The statistical method of the test results comprises the following steps:
TABLE 1 whether example Classification Table is adopted
TABLE 2 sample usage determination of whether to use case classification
| Status of use | Whether to employ data |
| During the test period, more than 3/4 are used | By using |
| During the test, the general method | By using |
| Less than half of the test period | Conference for others |
| Is not substantially used | Without using |
| Otherwise, the usage and dosage do not comply with | Conference for others |
9. The data statistical analysis method comprises the following steps: the SPSS software is used to make descriptive statistics on the various measurements, including quantity, mean, standard deviation, minimum, maximum, etc. The significance test of the normal distribution of the data improvement value is carried out by Shapiro-wilk test (Charcot-Wilk test), the data improvement value is normally distributed if Sig is more than 0.01 (double sides), and the significance difference level alpha is 0.05 after the T test is carried out. If weak Sig (both sides) <0.01, the distribution is abnormal, Wilcoxon test is carried out, the significance difference level alpha is 0.05, the significance difference level is P value <0.05, which shows that statistical difference exists, P <0.01, and the statistical difference is obvious.
10. Analysis of Experimental results
10.1T group test item result data and analysis
(1) Summary of data for T-group test items 0 weeks (W0) and 8 weeks (W8)
TABLE 3 data of T-group test items at week 0 (W0) and week 8 (W8)
**P≤0.05，***P≤0.01。
(2) Analysis of results of T-group experiments
The test results (table 3) for this group of subjects (30) after 8 weeks on placebo show that:
-moisture: compared with the prior art, after the test subjects of the group continuously use the product for 8 weeks, the product has certain moisturizing effect (P is 0.06), and the moisture increasing rate is 3.5%.
-wrinkles: compared with the prior use, the total size of the wrinkles is reduced by 22% (P is less than or equal to 0.05) and the depth of the wrinkles is reduced by 21% (P is 0.8) after the group of subjects continuously use for 8 weeks.
Pores, no statistical difference at each time point.
Texture no statistical difference at each time point.
10.2S group test item result data and analysis
(1) Data summary of the S test items 0 week (W0) and 8 weeks (W8)
TABLE 4 data of week 0 (W0) and week 8 (W8) S test items
**P≤0.05，***P≤0.01。
(2) Analysis of results of S group experiments
After 8 weeks for this group of subjects (31), who used the wrinkle-removing essence without recombinant collagen, the test results showed that:
-moisture: compared with the prior art, after the test subjects of the group continuously use the product for 8 weeks, the product has certain moisturizing effect (P is less than or equal to 0.01), and the water lifting rate is 4%.
-wrinkles: compared with the data before use, the data have significant difference, and after the subjects in the group continuously use for 8 weeks, the improvement rate of the overall size of the wrinkles is 29.22 percent (P is less than or equal to 0.05), and the wrinkle depth is reduced by 25.4 percent (P is less than or equal to 0.05).
-pore: the improvement rate of pore volume in 8 weeks is 45.26% (P is less than or equal to 0.05). Pore density was not statistically varied.
-texture: the improvement rate of the texture is 20.32 percent (P is less than or equal to 0.01) in 8 weeks.
10.3 group A test item results data and analysis
(1) Summary of data for group A test items 0 weeks (W0) and 8 weeks (W8)
TABLE 5 week 0 (W0) and week 8 (W8) data of group A test items
(2) Analysis of group A Experimental results
After 8 weeks for this group of subjects (32 persons) who used the wrinkle-removing essence containing recombinant collagen, the test results showed that:
-moisture: compared with the prior art, after the test subjects of the group continuously use for 8 weeks, the product has certain moisturizing effect (P is less than or equal to 0.01), the water lifting rate is 5.64 percent,
-wrinkles: compared with the prior art, the total size of the wrinkles of the subjects in the group after 8 weeks of continuous use has obvious change in 8 weeks, (P is less than or equal to 0.01), and the wrinkles have statistical significance, the improvement rate is 31.35%, the improvement rate of the wrinkle depth is 26.03% at 8 weeks, (P is less than or equal to 0.01).
-pore: the pore volume changes obviously in week 8, the P values are all less than 0.01, the statistical significance is achieved, and the improvement rate is 45.95%. The pore density is less than or equal to 0.05 at week 8, and the improvement rate is 58.15%.
-texture: the skin texture had a P value of less than 0.01 at week 8 with a percent improvement of 10.8%.
The comparative analysis of the detection item data of the T group, the S group and the a group shows that:
moisture content: the moisture content of the wrinkle-removing essence group A containing the recombinant collagen is higher than that of the wrinkle-removing essence group S and the placebo group T which do not contain the recombinant collagen in the 8 th week, and the moisture content of the wrinkle-removing essence group S containing the recombinant collagen is higher than that of the group T.
Wrinkling: the total size of wrinkles and the depth of wrinkles are in week 8, the group A of the wrinkle-removing essence containing the recombinant collagen is superior to the group S of the wrinkle-removing essence not containing the recombinant collagen and the group T of the placebo, the group A of the wrinkle-removing essence containing the recombinant collagen has a statistical difference in P value, and the group S of the wrinkle-removing essence not containing the recombinant collagen is superior to the group T of the placebo.
Pores, the wrinkle-removing essence group A containing the recombinant collagen has obvious change in the volume and density of the pores around 8, and is superior to the wrinkle-removing essence group S and the placebo group T which do not contain the recombinant collagen, and the wrinkle-removing essence group S containing the recombinant collagen has the improvement rate in the volume and density of the pores around 8 which is superior to the placebo group T (P is less than or equal to 0.05).
Texture: the wrinkle-removing essence A group containing recombinant collagen has a texture improvement rate of 10.80% (P is less than or equal to 0.01) at 8 weeks, and the wrinkle-removing essence S group containing no recombinant collagen has a texture improvement rate of 20.32% (P is less than or equal to 0.01) at 8 weeks.
In conclusion, compared with the placebo group T, the wrinkle removing essence group S without the recombinant collagen has the effects of improving wrinkles, textures, moisture and pores, and the wrinkle removing essence without the recombinant collagen also has the effects of removing wrinkles, moisturizing, shrinking pores and tightening. However, the wrinkle size and depth, pore improvement rate and moisture of the group A of the wrinkle-removing essence containing the recombinant collagen are higher than those of the group S of the wrinkle-removing essence and the group T of the placebo cream which do not contain the recombinant collagen, which shows that the recombinant collagen has the effects of removing wrinkles, shrinking pores and moisturizing, and the wrinkle-removing essence containing the recombinant collagen has a better effect. The recombinant collagen adopted in the embodiment is fermented from pichia pastoris, is stable and single in component, safe and free of allergy to human skin, is the collagen required by human body, can supplement the collagen lost by the skin, achieves the effects of removing wrinkles, smoothing wrinkles and moisturizing, and enables the skin to look young.
Claims (1)
1. The wrinkle-removing essence containing recombinant collagen is characterized by comprising the following components in percentage by mass: 0.8% of recombinant collagen, 30% of glycerol, 2.5% of 1, 2-hexanediol, 0.5% of sodium hyaluronate, 0.5% of polyglutamic acid, 0. 80.05% of acetyl hexapeptide, 0.06% of elastin, 0.03% of fibronectin and the balance of water, wherein the recombinant collagen is Pichia pastoris with the preservation number of CGMCC number 5021Pichia pastorisAnd (4) fermenting and producing.
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| CN116270295B (en) * | 2023-04-07 | 2023-11-10 | 广东丸美生物技术股份有限公司 | Anti-wrinkle composition containing fully-humanized collagen and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102443057A (en) * | 2011-10-26 | 2012-05-09 | 南京理工大学 | Recombinant humanized collagen and its preparation method |
-
2019
- 2019-12-26 CN CN201911363499.1A patent/CN110934766B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102443057A (en) * | 2011-10-26 | 2012-05-09 | 南京理工大学 | Recombinant humanized collagen and its preparation method |
Non-Patent Citations (2)
| Title |
|---|
| "含活性肽护肤霜的抗衰老功效测试与分析";倪梦嘉等;《香料香精化妆品》;20190831(第4期);第52-55页 * |
| "薇莱芙 类人胶原蛋白冻干球精华液";江苏江山聚源生物技术有限公司;《http://ftba.nmpa.gov.cn:8181/ftban/itownet/hzp_ba/fw/pz.jsp?processid=201809211407210guf1&nid=201809211407210guf1》;20180925;第2页 * |
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Denomination of invention: Anti wrinkle essence containing recombinant collagen Effective date of registration: 20230404 Granted publication date: 20220412 Pledgee: Jiangsu Jingjiang Rural Commercial Bank Co.,Ltd. Pledgor: JIANGSU JLAND BIOTECH Co.,Ltd. Registration number: Y2023980037241 |
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