CN111700818A - Wrinkle-removing and anti-aging ginseng polypeptide toning lotion and preparation method thereof - Google Patents
Wrinkle-removing and anti-aging ginseng polypeptide toning lotion and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/68—Sphingolipids, e.g. ceramides, cerebrosides, gangliosides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/645—Proteins of vegetable origin; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/673—Vitamin B group
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
Abstract
The invention provides a wrinkle-removing and anti-aging ginseng polypeptide toning lotion which is prepared from the following raw materials in percentage by weight: 5-15% of ceramide, 7-10% of nicotinamide, 30-40% of ginseng polypeptide liquid, 10-20% of collagen peptide, 5-10% of EDTA and the balance of deionized water. The ginseng polypeptide liquid with high activity and high purity prepared by the invention has the advantages that the natural activity of ginseng polypeptide is kept, the ginseng polypeptide liquid can be used as active bioactive macromolecules to activate the activity of human cells, the bioavailability is good, the ginseng polypeptide liquid and the collagen liquid can improve the capability of breaking through the human epidermal barrier through molecular filtration, the biological permeability is greatly improved, the addition of the ginseng polypeptide liquid and the collagen liquid has the synergistic effect, and the prepared lotion has good wrinkle-removing and anti-aging effects and is popular with consumers.
Description
Technical Field
The invention relates to the technical field of cosmetics, and in particular relates to a wrinkle-removing and anti-aging ginseng polypeptide toning lotion and a preparation method thereof.
Background
The skin is the largest organ in human body area, covers the whole body, and is composed of epidermis, dermis and subcutaneous tissue. The skin is the first "firewall" of the human body to the adverse environment outside the body, and protects various tissues and organs in the body from physical, mechanical, chemical and pathogenic microorganisms, and has two barrier functions: on one hand, the water, electrolyte and other substances in the body are prevented from losing; on the other hand, the invasion of the outside harmful substances is prevented. The skin of the baby has the collagen content in the dermis as high as 80 percent, so the baby is delicate, moist, fine, pure and elastic; with the increase of age, collagen begins to run off at the age of 18-22 years, and tiny pores appear on two cheeks; the collagen content in the skin dermis of a 25-year-old female is 65%, fine lines appear in canthus and mouth corners, the elasticity is reduced, and the skin is dry; at the age of 30-35, the collagen loss is nearly 20%, wrinkles appear at canthus, pores are larger, and skin problems such as color spots, dryness, tarnish and the like can appear; at the age of 40-45, deep wrinkles appear on both sides of the corner of the mouth and the forehead, the skin begins to collapse and is hastened, even the facial contour is changed, and at the moment, the loss of collagen in the dermis reaches nearly 40%. It can be said that the aging process of the skin is a process in which collagen is gradually lost. With the gradual loss of moisturizing factors such as collagen, the moisture content between skin tissue cells and among cells is reduced, so that the cells are tightly arranged, the peptide bonds and the elastic net of collagen supporting the skin are broken, the spiral net structure of the collagen is broken, the skin tissue is oxidized, atrophied and collapsed, and the skin can have aging phenomena such as dryness, wrinkles, looseness, no elasticity and the like. Also, according to the aging free radical theory, many substances in the body generate free radicals during metabolism, and excessive free radicals cause body damage, thereby causing and accelerating aging. People who love beauty have much attention, especially women pay more attention, and people have been pursuing to beautify their own cosmetics since ancient times.
With the rapid development and wide application of modern science and technology, cosmetics have become an indispensable daily use in people's lives from luxury goods with the continuous improvement of living standards. The requirements on cosmetics are also increasing and tend to be natural, nutritional, safe and non-irritant, and the cosmetics are developed from basic skin care products for cleaning and moistening skin to functional cosmetics for delaying senescence, removing wrinkles and beautifying skin. The basic care of skin mainly comprises three steps of cleaning, activating and protecting skin, and the toning lotion is used as a key role of 'starting and finishing' in the basic care three-step yeast, and is an essential article for daily beauty and skin protection. It can be used for cleaning the residue of the face cleaning product on the skin, can regulate the acid-base balance of the skin, enables the skin to be in the best state of absorbing nutrition, quickly replenishes moisture and nutrition for the skin, and cleans oil and light to astringe pores.
Ginseng has been known as the "king of all herbs" in ancient times, and is also known as the "nourishing yin, tonifying life, strengthening body resistance and consolidating constitution" by the oriental medical community. The ginseng contains various saponin and polysaccharide components, the ginseng leachate can be slowly absorbed by the skin, has no any adverse stimulation to the skin, can expand skin capillary vessels, promote skin blood circulation, increase skin nutrition, regulate water-oil balance of the skin, prevent skin dehydration, hardening and wrinkling, and can enhance skin elasticity and regenerate cells by using the ginseng-containing product for a long time. Meanwhile, the ginseng active substance also has the function of inhibiting the reduction of melanin, so that the skin is white and smooth. Its beauty effect is not good, and it is an excellent product for skin care and beauty. The hair conditioner can expand the capillary vessels of the head, increase the nutrition of the hair, improve the toughness of the hair, and reduce the hair loss and the hair breakage, and has the protection effect on the damaged hair. The oral administration of Ginseng radix not only can strengthen body constitution, but also has antiaging, skin caring and skin caring effects. The enzymolysis reaction of protease is a common method for preparing peptide, and the method can be used for directly producing peptide products in vitro. The method has the advantages that firstly, the enzymolysis degree can be controlled by controlling the enzymolysis time and the ratio of enzyme to substrate, and the polypeptide can be generated by a hydrolytic splitting method under the optimal condition temperature and milder pH value, so that the condition requirements for producing the bioactive peptide can be better met; secondly, with the continuous development of gene engineering and fermentation industry, the mature preparation and purification process greatly improves the activity and yield of enzyme, and the higher production cost is rapidly reduced; in addition, the method for producing active peptides by enzymatic hydrolysis of proteins is extremely safe.
There are many reports on the research of the lotion at present, for example, chinese patent application with publication number CN101966135A discloses a lotion containing nano skin-moistening components, comprising: 0.1-20% of surfactant, 0.1-30% of polyalcohol, 0.1-20% of emollient, 0.1-30% of humectant, 0.001-3% of preservative, 0.01-15% of other additives and 50-98% of water; the grain diameter of the emollient of the toning lotion is less than 200 nm; the surfactant is a nonionic surfactant, and comprises one or more than one of polyglycerol-2-oleate, sorbitan stearate, sorbitol laurate, dimethicone copolyol, cetostearyl glucose ester, polysorbate, and sorbitan fatty acid ester; the emollient is a component with skin moistening effect, and includes but is not limited to one or more of lecithin, hydrogenated lecithin, shea butter, dioctyl carbonate, jojoba oil, dimethicone, caprylic/capric triglyceride, cetyl isooctanoate, squalane, and octyl methoxycinnamate; the preservative is a component with the putrefaction effect caused by the growth delay or chemical change of microorganisms, and comprises one or more than one of methyl paraben, propyl paraben, DMDM hydantoin, imidazolidinyl urea, phenoxyethanol and p-hydroxybenzoate sodium salt; the lotion can make up the defect that common lotion cannot effectively moisturize skin, can also realize the defect that common lotion and cream cannot rapidly moisturize skin, has the characteristics of simple preparation process, fresh skin feeling and the like, and can simplify skin care steps for users. The Chinese patent application with the publication number of CN101234132A discloses the use of angelica sinensis or an extract thereof in preparing skin care products, wherein the angelica sinensis or the extract thereof has the functions of regulating the secretion function of skin, moisturizing, preventing and treating color spots and promoting the metabolism of the skin, and has obvious effects of improving the skin color and health state of the skin by taking the angelica sinensis or the extract thereof as a main active ingredient in the skin care products.
Therefore, the ginseng polypeptide toning lotion which is safe and environment-friendly and has the functions of removing wrinkles and resisting aging has wide market prospect.
Disclosure of Invention
The invention aims to provide a wrinkle-removing and anti-aging ginseng polypeptide lotion and a preparation method thereof, the prepared high-activity and high-purity ginseng polypeptide liquid has the natural activity of ginseng polypeptide, can be used as active bioactive macromolecules to activate the activity of human cells, has good bioavailability, can improve the capability of breaking through the human epidermal barrier and greatly improve the biological permeability of the human epidermal barrier by molecular filtration of the ginseng polypeptide liquid and the collagen liquid, and has a synergistic effect by adding the ginseng polypeptide liquid and the collagen liquid, and the prepared lotion has good wrinkle-removing and anti-aging effects.
The technical scheme of the invention is realized as follows:
the invention provides a wrinkle-removing and anti-aging ginseng polypeptide toning lotion, which comprises the following raw materials: ceramide, nicotinamide, ginseng polypeptide liquid, collagen peptide, EDTA and deionized water.
As a further improvement of the invention, the health-care food is prepared from the following raw materials in percentage by weight: 5-15% of ceramide, 7-10% of nicotinamide, 30-40% of ginseng polypeptide liquid, 10-20% of collagen peptide, 5-10% of EDTA and the balance of deionized water.
As a further improvement of the invention, the health-care food is prepared from the following raw materials in percentage by weight: 10% of ceramide, 8% of nicotinamide, 35% of ginseng polypeptide liquid, 15% of collagen peptide, 9% of EDTA and the balance of deionized water.
As a further improvement of the invention, the ginseng polypeptide liquid is prepared by the following method:
s1, pretreatment: drying the flowers, leaves and roots of the ginseng at the temperature of 105-120 ℃ for 0.5-4h to obtain a raw material A with the water content of not higher than 7%;
s2, crushing: crushing the raw material A to 50-100 meshes to obtain powder B;
s3, drying: drying the powder B at the temperature of 105-120 ℃ for 0.5-1h to obtain powder C with the water content of less than 5%;
s4, extraction: performing supercritical carbon dioxide extraction on the powder C, performing primary extraction, and adding an entrainer during extraction; extracting to obtain liquid D and residue E, and extracting the residue E for the second time to obtain extract;
s5, separation: mixing the obtained extract with liquid D to analyze ginseng polypeptide, and recovering solvent F; heating the analyzed Ginseng radix polypeptide to 70-80 deg.C, maintaining for more than 10min, homogenizing in a homogenizer, and bottling or performing the next step;
s6, filtering: filtering the separated ginseng polypeptide liquid by adopting an ultra-micro filtration membrane with the molecular weight cut-off of 80-120 kD;
s7, disinfection: flowing the filtered ginseng polypeptide liquid through the irradiation of an ultraviolet lamp.
As a further improvement of the present invention, the weight ratio of the entrainer to the powder C in step S4 is (0.3-1): 100, respectively; the temperature of the first extraction is 20-50 ℃, the pressure is 5-30MPa, the extraction time is 1-5h, and the flow rate of the ethanol is 500-; the temperature of the second extraction is 20-50 ℃, the pressure is 5-30MPa, the extraction time is 1-5.5h, and the flow rate of carbon dioxide is 500-850 kg/h; the entrainer is one or a mixture of more than two of ethyl acetate, gasoline, petroleum ether, ethanol and water.
As a further improvement of the invention, the ginseng polypeptide precipitation conditions in the step S5 are that the temperature is 20-50 ℃ and the pressure is 1-15 MPa; the homogenization condition is 10000-12000r/min homogenization for 1-2 min.
As a further improvement of the invention, the irradiation dose of the ultraviolet lamp in the step S7 is 200-250J/m2The irradiation time is 30-60 s; the flow rate of the ginseng polypeptide liquid is 2-5 mL/min.
As a further improvement of the invention, the collagen peptide is selected from one or a mixture of more than two of deep sea cod skin collagen peptide, pig skin collagen peptide, sheep skin collagen peptide, cow skin collagen peptide, pig bone collagen peptide, sheep bone collagen peptide and cow bone collagen peptide; the molecular weight of the collagen peptide is between 500 and 1000D.
The invention further provides a preparation method of the wrinkle-removing and anti-aging ginseng polypeptide toning lotion, which comprises the following steps: weighing ceramide and nicotinamide, adding deionized water, stirring, adding Ginseng radix polypeptide solution and collagen peptide, heating to 40-50 deg.C, stirring for dissolving, slowly adding EDTA to adjust pH to 5-5.5, and stirring; adding active carbon according to solid-to-liquid ratio of 0.01-0.02g/mL, stirring for 25min, and coarse filtering to remove carbon; filtering with 0.22 μm filter membrane to obtain fine filtrate; encapsulating, wherein the filling amount of each bottle is 2 mL; sterilizing, inspecting, packaging, and warehousing.
As a further improvement of the invention, the sterilization method is to sterilize for 15-20min at 110-120 ℃ under 1.5-2 MPa.
The invention has the following beneficial effects: the added collagen peptide has the characteristics of nutrition, repairability, heat preservation, compatibility, affinity and the like, is similar to the structure of human skin collagen, has good compatibility, has interaction of ionic bonds, hydrogen bonds and the like, can be diffused to the deep layer of the skin, and has good nutrition effect on the skin of people; meanwhile, the molecules of the composite material contain a large number of hydroxyl groups, so that the composite material has a good heat preservation effect; the amino and carboxyl groups in the molecule endow the fabric with certain surface activity, so that the fabric has good compatibility with other materials and has no irritation to skin. After entering human body, macromolecular collagen needs to be degraded into collagen peptide with molecular weight less than 1000 daltons and amino acid to be absorbed by human body. The collagen with smaller molecular weight is easier to absorb, but is changed into free amino acid with smaller molecular weight, the proportion of the collagen is too large and is not the most ideal product, the collagen with 500-1000 Dalton adopted in the application is the short peptide which is most easily absorbed, the collagen with the molecular weight range has the strongest activity, the absorption effect after the use is also the best, and the collagen can be quickly fused into the dermis to supplement the collagen which is urgently needed by the body, and can really enter the dermis to help repair the skin and rebuild the collagen layer.
The invention adopts a supercritical fluid extraction method to prepare high-activity high-purity ginseng polypeptide liquid, an entrainer selected from ethyl acetate, gasoline, petroleum ether, ethanol and water is added in the extraction process, according to the principle of similar intermiscibility, the extraction of oil-soluble and water-soluble substances in raw materials can be promoted, the extracted substances contain rich protein substances, the ginseng polypeptide with natural activity is obtained by further analysis, the ginseng polypeptide can be used as active bioactive macromolecules to activate the activity of human cells, and has good bioavailability, the ginseng polypeptide liquid and the collagen liquid can improve the capability of breaking through the human epidermal barrier by molecular filtration, the biological permeability of the ginseng polypeptide liquid and the collagen liquid is greatly improved, and have good functions of anti-aging, anti-wrinkle and anti-oxidation, the addition of the ginseng polypeptide liquid and the collagen liquid has the effect of synergy, and the prepared toning lotion has good effects of wrinkle and anti-aging, is popular with consumers.
The invention has simple process, lower cost and stable quality, selects natural raw materials which are rich in resources and are rarely applied to the prior cosmetic water products from the selection of functional raw materials of the cosmetic water, can quickly absorb the cosmetic water by the skin, can deeply nourish the skin and recover the natural elasticity, the skin care product is soft and smooth as velvet without oily feeling, effectively utilizes active substances and nutrient elements in raw materials, organically combines and synergizes a plurality of active ingredients, directly acts on a real leather layer causing skin aging, quickly supplements various nutrients and moisture required by the skin, increases the vitality of skin cells, enhances the regeneration function of epidermal cells, repairs fine lines of the skin, makes the skin compact and bright, realizes the moisturizing, whitening, wrinkle removing, freckle removing and aging delaying of the skin, and reproduces the healthy, fine and smooth natural beauty, smoothness and smoothness of the skin.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, are within the scope of the present invention.
The collagen peptide content of the deep sea cod skin in the examples is more than 90%, and the batch number is 20191022; pigskin collagen peptide, the collagen peptide content is greater than 90%, lot number 20200115; the pig bone collagen peptide has the collagen peptide content of more than 90 percent and the batch number of 20190720; all purchased from Shandong Nuanli Biotech Co., Ltd;
ceramide, CAS number 100403-19-8; nicotinamide, CAS number 98-92-0; purchased from santa alba biotechnology limited, Hubei province.
Ginseng, Changde peach source in Hunan province, purchased from Lichang agricultural development Co., Ltd, Chongqing city.
Example 1 wrinkle-removing anti-aging ginseng polypeptide lotion
The raw materials comprise (by weight percentage) 5% of ceramide, 7% of nicotinamide, 30% of ginseng polypeptide liquid, 10% of collagen peptide, 5% of EDTA and the balance of deionized water.
The extraction method of the ginseng polypeptide liquid comprises the following steps: pretreatment: drying the flowers, leaves and roots of ginseng at 105 ℃ for 0.5h to obtain a raw material A with the water content of 7%; crushing: crushing the raw material A to 50 meshes to obtain powder B; and (3) drying: drying the powder B at 105 ℃ for 1h to obtain powder C with the water content of less than 5%; and (3) extraction: performing supercritical carbon dioxide extraction on the powder C, wherein the extraction temperature is 20 ℃, the pressure is 5MPa, the extraction time is 1h, the flow rate of ethanol is 500kg/h, an entrainer ethyl acetate is also added during extraction, and the weight ratio of the entrainer to the powder C is 0.3: 100, respectively; extracting to obtain liquid D and residue E, and performing secondary extraction on the residue E at 20 ℃ and 5MPa for 1h at a carbon dioxide flow rate of 500kg/h to obtain an extract; separation: mixing the obtained extract with liquid D, and resolving Ginseng radix polypeptide at 20 deg.C under 1MPa, and recovering solvent F; heating the analyzed ginseng polypeptide to 70 deg.C, maintaining for more than 10min, homogenizing in a homogenizer under the condition of 10000r/min for 1min, and filling or performing the next step; and (3) filtering: filtering the separated ginseng polypeptide liquid by adopting an ultra-micro filtration membrane with the molecular weight cut-off of 80-120 kD; and (3) disinfection: allowing the filtered ginseng polypeptide liquid to flow through the ultraviolet lamp for irradiation with an irradiation dose of 250J/m2The irradiation time was 30 seconds.The ginseng polypeptide liquid obtained in the embodiment comprises 24.6% of ginseng polypeptide, 56.2% of unsaturated fatty acid and 10.4% of terpenoid by weight.
The collagen peptide is pigskin collagen peptide, and the molecular weight is between 500-1000D.
The preparation method of the wrinkle-removing and anti-aging ginseng polypeptide toning lotion comprises the following steps: weighing ceramide and nicotinamide, adding deionized water, stirring, adding Ginseng radix polypeptide solution and collagen peptide, heating to 40 deg.C, stirring for dissolving, slowly adding EDTA to adjust pH to 5, and stirring; adding active carbon according to solid-to-liquid ratio of 0.01g/mL, stirring for 25min, and coarse-filtering to remove carbon; filtering with 0.22 μm filter membrane to obtain fine filtrate; encapsulating, wherein the filling amount of each bottle is 2 mL; sterilizing (sterilization method is hot-pressing sterilization at 110 deg.C and 1.5MPa for 15min), inspecting, packaging, and storing.
Example 2
The raw materials comprise (by weight percentage) 15% of ceramide, 10% of nicotinamide, 40% of ginseng polypeptide liquid, 20% of collagen peptide, 10% of EDTA and the balance of deionized water.
The extraction method of the ginseng polypeptide liquid comprises the following steps: pretreatment: drying the flowers, leaves and roots of the ginseng at 120 ℃ for 4 hours to obtain a raw material A with the water content of less than 7%; crushing: crushing the raw material A to 100 meshes to obtain powder B; and (3) drying: drying the powder B at 120 ℃ for 0.5h to obtain powder C with the water content of less than 5%; and (3) extraction: performing supercritical carbon dioxide extraction on the powder C, wherein the extraction temperature is 50 ℃, the pressure is 30MPa, the extraction time is 5h, the flow of carbon dioxide is 850kg/h, an entrainer solvent gasoline is also added during extraction, and the weight ratio of the entrainer to the powder C is 1.0: 100, respectively; extracting to obtain liquid D and residue E, performing secondary extraction on the residue E at 50 ℃ and 30MPa for 5.5h, wherein the flow of carbon dioxide is 850kg/h to obtain an extract; separation: mixing the obtained extract with liquid D, and resolving Ginseng radix polypeptide at 50 deg.C under 15MPa, and recovering solvent F; heating the analyzed Ginseng radix polypeptide to 80 deg.C, maintaining for more than 10min, homogenizing in a homogenizer at 12000r/min for 2min, and bottling after homogenizing or processing in the next stepC, processing; and (3) filtering: filtering the separated ginseng polypeptide liquid by adopting an ultra-micro filter membrane with the cut-off molecular weight of 80-120 kD; and (3) disinfection: allowing the filtered ginseng polypeptide liquid to flow through the ultraviolet lamp for irradiation with irradiation dose of 200J/m2The irradiation time was 60 seconds. The ginseng polypeptide liquid obtained in the embodiment comprises, by weight, 22.9% of ginseng polypeptide, 53.5% of unsaturated fatty acid and 10.6% of terpenoid.
The collagen peptide is porcine bone collagen peptide, and the molecular weight is between 500-1000D.
The preparation method of the wrinkle-removing and anti-aging ginseng polypeptide toning lotion comprises the following steps: weighing ceramide and nicotinamide, adding deionized water, stirring, adding Ginseng radix polypeptide solution and collagen peptide, heating to 50 deg.C, stirring for dissolving, slowly adding EDTA to adjust pH to 5.5, and stirring; adding active carbon according to the solid-to-liquid ratio of 0.02g/mL, stirring for 25min, and coarsely filtering to remove carbon; filtering with 0.22 μm filter membrane to obtain fine filtrate; encapsulating, wherein the filling amount of each bottle is 2 mL; sterilizing (sterilization method is sterilizing at 120 deg.C under 2MPa for 20min), inspecting, packaging, and storing.
Example 3
The raw materials comprise (by weight percentage) 10% of ceramide, 8% of nicotinamide, 35% of ginseng polypeptide liquid, 15% of collagen peptide, 9% of EDTA and the balance of deionized water.
The extraction method of the ginseng polypeptide liquid comprises the following steps: pretreatment: drying the flowers, leaves and roots of ginseng at 112 ℃ for 2 hours to obtain a raw material A with the water content of 6%; crushing: crushing the raw material A to 75 meshes to obtain powder B; and (3) drying: drying the powder B at 109 ℃ for 42min to obtain powder C with the water content of less than 5%; and (3) extraction: performing supercritical carbon dioxide extraction on the powder C, wherein the extraction temperature is 35 ℃, the pressure is 20MPa, the extraction time is 3h, the flow of carbon dioxide is 600kg/h, an entrainer petroleum ether is also added during extraction, and the weight ratio of the entrainer to the powder C is 0.7: 100, respectively; extracting to obtain liquid D and residue E, performing secondary extraction on the residue E at 35 ℃ and 20MPa for 3h, wherein the flow of carbon dioxide is 650kg/h to obtain an extract; separation: mixing the obtained extractive solution with liquid D at 35 deg.C and pressureResolving ginseng polypeptide under 12MPa, and recovering solvent F; heating the analyzed Ginseng radix polypeptide to above 70 deg.C, maintaining for above 10min, homogenizing in a homogenizer at 11000r/min for 1.5min, and bottling or performing the next step; and (3) filtering: filtering the separated ginseng polypeptide liquid by adopting an ultramicro filter membrane with the cut-off molecular weight of 80-120 kD; and (3) disinfection: allowing the filtered ginseng polypeptide liquid to flow through an ultraviolet lamp for irradiation with an irradiation dose of 225J/m2The irradiation time was 43 s. The ginseng polypeptide liquid obtained in the embodiment comprises, by weight, 31.3% of ginseng polypeptide, 52.4% of unsaturated fatty acid and 9.1% of terpenoid.
The collagen peptide is collagen peptide of deep sea cod skin, and the molecular weight is between 500 and 1000D.
The preparation method of the wrinkle-removing and anti-aging ginseng polypeptide toning lotion comprises the following steps: weighing ceramide and nicotinamide, adding deionized water, stirring, adding Ginseng radix polypeptide solution and collagen peptide, heating to 45 deg.C, stirring for dissolving, slowly adding EDTA to adjust pH to 5.2, and stirring; adding active carbon according to the solid-to-liquid ratio of 0.015g/mL, stirring for 25min, and carrying out rough filtration to remove carbon; filtering with 0.22 μm filter membrane to obtain fine filtrate; encapsulating, wherein the filling amount of each bottle is 2 mL; sterilizing (sterilization method comprises autoclaving at 118 deg.C and 1.75MPa for 18min), inspecting, packaging, and storing.
Comparative example 1
Compared with example 3, the ginseng polypeptide solution is not added, and other conditions are not changed.
The raw materials comprise (by weight percentage) 10% of ceramide, 8% of nicotinamide, 50% of collagen peptide, 9% of EDTA and the balance of deionized water.
The collagen peptide is collagen peptide of deep sea cod skin, and the molecular weight is between 500 and 1000D.
The preparation method of the wrinkle-removing and anti-aging ginseng polypeptide toning lotion comprises the following steps: weighing ceramide and nicotinamide, adding deionized water, stirring, adding Ginseng radix polypeptide solution and collagen peptide, heating to 45 deg.C, stirring for dissolving, slowly adding EDTA to adjust pH to 5.2, and stirring; adding active carbon according to the solid-to-liquid ratio of 0.015g/mL, stirring for 25min, and carrying out rough filtration to remove carbon; filtering with 0.22 μm filter membrane to obtain fine filtrate; encapsulating, wherein the filling amount of each bottle is 2 mL; sterilizing (sterilization method comprises autoclaving at 118 deg.C and 1.75MPa for 18min), inspecting, packaging, and storing.
Comparative example 2
Compared with example 3, no collagen peptide was added, and other conditions were not changed.
The raw materials comprise (by weight percentage) 10% of ceramide, 8% of nicotinamide, 50% of ginseng polypeptide liquid, 9% of EDTA and the balance of deionized water.
The extraction method of the ginseng polypeptide liquid comprises the following steps: pretreatment: drying the flowers, leaves and roots of ginseng at 112 ℃ for 2 hours to obtain a raw material A with the water content of 6%; crushing: crushing the raw material A to 75 meshes to obtain powder B; and (3) drying: drying the powder B at 109 ℃ for 42min to obtain powder C with the water content of less than 5%; and (3) extraction: performing supercritical carbon dioxide extraction on the powder C, wherein the extraction temperature is 35 ℃, the pressure is 20MPa, the extraction time is 3h, the flow of carbon dioxide is 600kg/h, an entrainer petroleum ether is also added during extraction, and the weight ratio of the entrainer to the powder C is 0.7: 100, respectively; extracting to obtain liquid D and residue E, performing secondary extraction on the residue E at 35 ℃ and 20MPa for 3h, wherein the flow of carbon dioxide is 650kg/h to obtain an extract; separation: mixing the obtained extract with liquid D, and resolving Ginseng radix polypeptide at 35 deg.C under 12MPa, and recovering solvent F; heating the analyzed Ginseng radix polypeptide to above 70 deg.C, maintaining for above 10min, homogenizing in a homogenizer at 11000r/min for 1.5min, and bottling or performing the next step; and (3) filtering: filtering the separated ginseng polypeptide liquid by adopting an ultramicro filter membrane with the cut-off molecular weight of 80-120 kD; and (3) disinfection: allowing the filtered ginseng polypeptide liquid to flow through an ultraviolet lamp for irradiation with an irradiation dose of 225J/m2The irradiation time was 43 s. The ginseng polypeptide liquid obtained in the embodiment comprises, by weight, 31.3% of ginseng polypeptide, 52.4% of unsaturated fatty acid and 9.1% of terpenoid.
The preparation method of the wrinkle-removing and anti-aging ginseng polypeptide toning lotion comprises the following steps: weighing ceramide and nicotinamide, adding deionized water, stirring, adding Ginseng radix polypeptide solution, heating to 45 deg.C, stirring for dissolving, slowly adding EDTA to adjust pH to 5.2, and stirring; adding active carbon according to the solid-to-liquid ratio of 0.015g/mL, stirring for 25min, and carrying out rough filtration to remove carbon; filtering with 0.22 μm filter membrane to obtain fine filtrate; encapsulating, wherein the filling amount of each bottle is 2 mL; sterilizing (sterilization method comprises sterilizing at 118 deg.C under 1.75MPa for 18min), inspecting, packaging, and storing.
Comparative example 3
Compared with example 3, the ginseng polypeptide liquid is replaced by an aqueous solution with the content of the ginseng polypeptide of 31.3 wt% prepared by common ginseng polypeptide (purchased from Shaanxi Tenuimai Biotech Limited liability company).
The raw materials comprise (by weight percentage) 10% of ceramide, 8% of nicotinamide, 35% of common ginseng polypeptide liquid, 15% of collagen peptide, 9% of EDTA and the balance of deionized water.
Common ginseng polypeptide liquid: is prepared from water solution of ginseng polypeptide (purchased from Shanxi Teng Mei Biotech, Ltd.) with content of 31.3 wt% prepared from common ginseng polypeptide in deionized water.
The collagen peptide is collagen peptide of deep sea cod skin, and the molecular weight is between 500 and 1000D.
The preparation method of the wrinkle-removing and anti-aging ginseng polypeptide toning lotion comprises the following steps: weighing ceramide and nicotinamide, adding deionized water, stirring, adding Ginseng radix polypeptide solution and collagen peptide, heating to 45 deg.C, stirring for dissolving, slowly adding EDTA to adjust pH to 5.2, and stirring; adding active carbon according to the solid-to-liquid ratio of 0.015g/mL, stirring for 25min, and carrying out rough filtration to remove carbon; filtering with 0.22 μm filter membrane to obtain fine filtrate; encapsulating, wherein the filling amount of each bottle is 2 mL; sterilizing (sterilization method comprises autoclaving at 118 deg.C and 1.75MPa for 18min), inspecting, packaging, and storing.
Test example 1 skin texture improvement assay
Skin texture was measured using lotions prepared in examples 1 to 3 of the present invention and comparative examples 1 to 3, respectively, and commercially available wrinkle-removing and aging-preventing lotions. Firstly, 70 female testees with healthy skin are selected, the left half face of the testee is coated with the toning lotion prepared by the invention to serve as an experimental group, and the other half face of the testee is not coated with any substance to serve as a blank group. The experiment is 8 weeks, the skin of the testee is detected and analyzed by various instruments at 1 week, 4 weeks and 8 weeks respectively, the improvement effects of the toning lotion on the moisture content of the skin, the elasticity of the skin, fine wrinkles, the roughness of the skin, the sebum secretion amount and the number of coarse and large pores are evaluated, and the average values of the three tests at 1 week, 4 weeks and 8 weeks are shown in table 1.
TABLE 1
Note: "+" is increasing and "-" is decreasing.
The results show (see table 1) that the toning lotion of the invention has different degrees of reduction on the fine lines, roughness, sebum secretion and number of coarse pores of the skin, has obvious improvement effect on the moisture content and the elasticity of the skin, and shows that the product of the invention has the effect of improving the skin quality of the skin.
In comparative example 1 and comparative example 2, the ginseng polypeptide solution or the collagen peptide was not added, respectively, and the skin-improving ability was significantly reduced, and thus, the addition of the ginseng polypeptide solution and the collagen peptide had a synergistic effect.
In the comparative example 3, the ginseng polypeptide liquid is replaced by the liquid prepared by the common ginseng polypeptide, and the effect of improving the skin is obviously reduced, so that the ginseng polypeptide liquid prepared by the invention has obvious effects of improving the fine lines, the roughness, the sebum secretion amount, the coarse and large pore number and the like of the skin.
Test example 2 measurement of anti-radical ability
The anti-DPPH/free radical ability was measured using the lotions prepared in examples 1 to 3 and comparative examples 1 to 3, respectively, and a commercially available wrinkle-removing and anti-aging lotion. First, 20mg of DPPH (1, 1-dimethylbenzyl phenylhydrazine, C) was accurately weighed18H12N5O6)Dissolving in 250mL volumetric flask with absolute ethanol, and adjusting DPPH concentration to 2 × 10-4mol/L. Several tubes were numbered and the reagents were added according to Table 2. Mixing, standing in dark at room temperature for 30min, and measuring absorbance at 517 nm. The same assay was repeated 3 times. DPPHRadical scavenging rate (%) - (1-A)Sample (I)-ABlank space)/AControl]×100％
ASample (I): absorbance of the sample at a wavelength of 517 nm.
ABlank space: absorbance of the blank at a wavelength of 517 nm.
AControl: comparison of the absorbance values at a wavelength of 517nm
TABLE 2
| Group of | DPPH(mL) | Sample (mL) | Anhydrous ethanol (mL) |
| Example 1 | 2.0 | 2.0 | 0 |
| Example 2 | 2.0 | 2.0 | 0 |
| Example 3 | 2.0 | 2.0 | 0 |
| Comparative example 1 | 2.0 | 2.0 | 0 |
| Comparative example 2 | 2.0 | 2.0 | 0 |
| Comparative example 3 | 2.0 | 2.0 | 0 |
| Blank group | 0 | 2.0 | 2.0 |
| Control group | 2.0 | 0 | 2.0 |
| Commercially available toning lotion | 2.0 | 2.0 | 0 |
The activity detection finds that: the toning lotion has excellent anti-free radical capability and good scavenging effect on DPPH & free radicals, and is shown in Table 3.
TABLE 3
Test example 3 anti-aging ability measurement
The lotions prepared in examples 1 to 3 and comparative examples 1 to 3 and commercially available anti-wrinkle and anti-aging lotions were used to measure anti-aging ability using SOD activity as an index.
Grouping and administration of experimental animals:
80 Kunming clean-grade mice, half male and female, with weight (20 +/-2) g, provided by the experimental animal center of Zhejiang university, were randomly divided into 8 groups (control group, sample group), and 10 mice each. The test interventions were performed on the lotions prepared in examples 1 to 3 and comparative examples 1 to 3 and on the commercial anti-wrinkle and anti-aging lotion by gavage the samples of the groups daily, starting 8:00 in the morning on the first day, and the mice were weighed 1 time every 1 day, with the gavage amount (10g/kg) adjusted accordingly to the body weight. The control group was also perfused with an equal amount of saline. After 1 month continuously, the patient is killed by decapitation, the brain is washed by cold physiological saline, and the cerebral cortex is peeled off for standby.
Index measurement:
the cerebral cortex is taken to be prepared into homogenate of 10 percent (mass percentage), and each index is measured according to the specification of an SOD test box.
The result of the detection
SOD activity detection results show that (see Table 4): the toning lotion prepared by the invention can obviously improve the SOD activity of the cerebral cortex of a mouse, enhances the capability of an organism for removing free radicals and lightens the attack of the free radicals on macromolecules of a biological membrane by improving the SOD activity of the cerebral cortex, thereby weakening the damage of excessive free radicals to the biological membrane, ensuring the integrity of the structure and the function of the cell membrane and delaying the occurrence of apoptosis.
TABLE 4
Note that: p <0.05 compared to placebo.
Although the toning lotion prepared in comparative examples 1-3 also has a certain anti-aging effect, the toning lotion is obviously lower than the product of the invention, the product of the invention is obviously higher than a blank control group, the addition of the ginseng polypeptide liquid and the collagen peptide has a good synergistic effect, and the ginseng polypeptide liquid prepared by the invention has a good anti-aging effect.
Compared with the prior art, the added collagen peptide has the characteristics of nutrition, repairability, heat preservation, compatibility, affinity and the like, is similar to the structure of human skin collagen, has good compatibility, has the interaction of ionic bonds, hydrogen bonds and the like, can be diffused to the deep layer of the skin, and has good nutrition effect on the skin of people; meanwhile, the molecules of the composite material contain a large number of hydroxyl groups, so that the composite material has a good heat preservation effect; the amino and carboxyl groups in the molecule endow the fabric with certain surface activity, so that the fabric has good compatibility with other materials and is not irritant to skin. After entering the human body, macromolecular collagen needs to be degraded into collagen peptide with the molecular weight less than 1000 daltons and amino acid to be absorbed by the human body. Collagen with a smaller molecular weight is more easily absorbed, but becomes too small as free amino acids, and its proportion is too large and is not an optimal product. The collagen which is most easily absorbed is the collagen of 500-1000 daltons, the collagen activity in the molecular weight range is the strongest, and the absorption effect is the best after the use. The micromolecule collagen can be rapidly blended into the dermis to supplement collagen which is urgently needed by the body, and can really enter the dermis to help repair the skin and rebuild the collagen layer.
The invention adopts a supercritical fluid extraction method to prepare high-activity high-purity ginseng polypeptide liquid, an entrainer selected from ethyl acetate, gasoline, petroleum ether, ethanol and water is added in the extraction process, according to the principle of similar intermiscibility, the extraction of oil-soluble and water-soluble substances in raw materials can be promoted, the extracted substances contain rich protein substances, the ginseng polypeptide with natural activity is obtained by further analysis, the ginseng polypeptide can be used as active bioactive macromolecules to activate the activity of human cells, and has good bioavailability, the ginseng polypeptide liquid and the collagen liquid can improve the capability of breaking through the human epidermal barrier by molecular filtration, the biological permeability of the ginseng polypeptide liquid and the collagen liquid is greatly improved, and have good functions of anti-aging, anti-wrinkle and anti-oxidation, the addition of the ginseng polypeptide liquid and the collagen liquid has the effect of synergy, and the prepared toning lotion has good effects of wrinkle and anti-aging, is popular with consumers.
The invention has simple process, lower cost and stable quality, selects natural raw materials which are rich in resources and are rarely applied to the prior cosmetic water products from the selection of functional raw materials of the cosmetic water, can quickly absorb the cosmetic water by the skin, can deeply nourish the skin and recover the natural elasticity, the skin care product is soft and smooth as velvet without oily feeling, effectively utilizes active substances and nutrient elements in raw materials, organically combines and synergizes a plurality of active ingredients, directly acts on a real leather layer causing skin aging, quickly supplements various nutrients and moisture required by the skin, increases the vitality of skin cells, enhances the regeneration function of epidermal cells, repairs fine lines of the skin, makes the skin compact and bright, realizes the moisturizing, whitening, wrinkle removing, freckle removing and aging delaying of the skin, and reproduces the healthy, fine and smooth natural beauty, smoothness and smoothness of the skin.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (10)
1. The wrinkle-removing and anti-aging ginseng polypeptide toning lotion is characterized by comprising the following raw materials: ceramide, nicotinamide, ginseng polypeptide liquid, collagen peptide, EDTA and deionized water.
2. The wrinkle-removing and anti-aging ginseng polypeptide toning lotion according to claim 1 is prepared from the following raw materials in percentage by weight: 5-15% of ceramide, 7-10% of nicotinamide, 30-40% of ginseng polypeptide liquid, 10-20% of collagen peptide, 5-10% of EDTA and the balance of deionized water.
3. The wrinkle-removing and anti-aging ginseng polypeptide toning lotion according to claim 2 is prepared from the following raw materials in percentage by weight: 10% of ceramide, 8% of nicotinamide, 35% of ginseng polypeptide liquid, 15% of collagen peptide, 9% of EDTA and the balance of deionized water.
4. The wrinkle-removing and anti-aging ginseng polypeptide lotion as claimed in claim 1, wherein the ginseng polypeptide lotion is prepared by the following method:
s1, pretreatment: drying the flowers, leaves and roots of the ginseng at the temperature of 105-120 ℃ for 0.5-4h to obtain a raw material A with the water content of not higher than 7%;
s2, crushing: crushing the raw material A to 50-100 meshes to obtain powder B;
s3, drying: drying the powder B at the temperature of 105-120 ℃ for 0.5-1h to obtain powder C with the water content of less than 5%;
s4, extraction: performing supercritical carbon dioxide extraction on the powder C, performing primary extraction, and adding an entrainer during extraction; extracting to obtain liquid D and residue E, and extracting the residue E for the second time to obtain extract;
s5, separation: mixing the obtained extract with liquid D to analyze ginseng polypeptide, and recovering solvent F; heating the analyzed Ginseng radix polypeptide to 70-80 deg.C, maintaining for more than 10min, homogenizing in a homogenizer, and bottling or performing the next step;
s6, filtering: filtering the separated ginseng polypeptide liquid by adopting an ultra-micro filter membrane with the cut-off molecular weight of 80-120 kD;
s7, disinfection: flowing the filtered ginseng polypeptide liquid through the irradiation of an ultraviolet lamp.
5. The wrinkle-removing and anti-aging ginseng polypeptide lotion as claimed in claim 4, wherein the weight ratio of the entrainer to the powder C in step S4 is (0.3-1): 100, respectively; the temperature of the first extraction is 20-50 ℃, the pressure is 5-30MPa, the extraction time is 1-5h, and the flow rate of the ethanol is 500-; the temperature of the second extraction is 20-50 ℃, the pressure is 5-30MPa, the extraction time is 1-5.5h, and the flow rate of carbon dioxide is 500-850 kg/h; the entrainer is one or a mixture of more than two of ethyl acetate, gasoline, petroleum ether, ethanol and water.
6. The wrinkle-removing and anti-aging ginseng polypeptide lotion as claimed in claim 4, wherein the ginseng polypeptide is precipitated under the conditions of temperature of 20-50 ℃ and pressure of 1-15MPa in step S5; the homogenization condition is 10000-12000r/min homogenization for 1-2 min.
7. The wrinkle-removing and anti-aging ginseng polypeptide lotion as claimed in claim 4, wherein the irradiation dose of the ultraviolet lamp in step S7 is 200-250J/m2The irradiation time is 30-60 s; the flow rate of the ginseng polypeptide liquid is 2-5 mL/min.
8. The wrinkle-removing and anti-aging ginseng polypeptide lotion as claimed in claim 1, wherein the collagen peptide is selected from one or a mixture of two or more of cod skin collagen peptide, pig skin collagen peptide, sheep skin collagen peptide, cow skin collagen peptide, pig bone collagen peptide, sheep bone collagen peptide and cow bone collagen peptide in deep sea; the molecular weight of the collagen peptide is between 500 and 1000D.
9. A method for preparing the wrinkle-removing and anti-aging ginseng polypeptide lotion as claimed in any one of claims 1 to 8, which comprises the following steps: weighing ceramide and nicotinamide, adding deionized water, stirring, adding Ginseng radix polypeptide solution and collagen peptide, heating to 40-50 deg.C, stirring for dissolving, slowly adding EDTA to adjust pH to 5-5.5, and stirring; adding active carbon according to solid-to-liquid ratio of 0.01-0.02g/mL, stirring for 25min, and coarse filtering to remove carbon; filtering with 0.22 μm filter membrane to obtain fine filtrate; encapsulating, wherein the filling amount of each bottle is 2 mL; sterilizing, inspecting, packaging, and warehousing.
10. The method as claimed in claim 9, wherein the sterilization is performed by autoclaving at 110 ℃ and 120 ℃ under 1.5-2MPa for 15-20 min.
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