CN111728919A - Firming and wrinkle-removing essence and preparation method thereof - Google Patents
Firming and wrinkle-removing essence and preparation method thereof Download PDFInfo
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- CN111728919A CN111728919A CN202010817628.6A CN202010817628A CN111728919A CN 111728919 A CN111728919 A CN 111728919A CN 202010817628 A CN202010817628 A CN 202010817628A CN 111728919 A CN111728919 A CN 111728919A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/37—Esters of carboxylic acids
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/60—Sugars; Derivatives thereof
- A61K8/602—Glycosides, e.g. rutin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/524—Preservatives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
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Abstract
The invention discloses a firming and wrinkle-removing essence which comprises the following raw materials in parts by weight: 3-7 parts of punicalagin, 1-5 parts of bearberry leaf polyphenol, 1-5 parts of cola nut extract, 10-15 parts of collagen peptide, 5-10 parts of acetyl dipeptide-1 cetyl palmitate, 5-10 parts of ethylhexyl palmitate, 1-3 parts of squalane, 1-3 parts of butanediol, 1-5 parts of emulsifier, 1-5 parts of thickener and 50-60 parts of deionized water; the collagen peptide is derived from fish scales, and the molecular weight of the collagen peptide is 1000-2000 Da. The punicalagin, the bearberry leaf polyphenol, the cola nut extract and the collagen peptide are added into the tightening and wrinkle-removing essence disclosed by the invention, so that exogenous collagen supplement and endogenous collagen synthesis promotion are combined, the content of collagen in skin is increased, the skin is firmer, the wrinkle volume on the surface of the skin is reduced, the wrinkle depth is reduced, the cola nut extract also has better corrosion resistance, no preservative is required to be added into the prepared essence, and the essence is safe and non-irritant to the skin.
Description
Technical Field
The invention relates to the field of cosmetics, and particularly relates to a firming and wrinkle-removing essence and a preparation method thereof.
Background
With the improvement of living standard, the requirements of people on the quality of life are higher and higher, and the facial appearance is more emphasized. Wrinkles are the most obvious and important feature of skin aging. Wrinkles in humans typically begin to appear around the age of 20 and gradually increase and deepen with age. With the age, the epidermis tissue of the skin becomes dry and thin, collagen and elastin gradually run off, the fine mesh structure formed by interweaving collagen fibers and elastic fibers is damaged, the skin is relaxed, and wrinkles with different thicknesses can appear.
Collagen is synthesized by fibroblasts, but as people age and free radicals are produced in large quantities, the structure of collagen is changed and gradually lost (normal human bodies lose 1% of collagen every year), so that the content and the type of collagen in the bodies are changed. Meanwhile, the synthetic capacity of fibroblasts is reduced, the degradation of collagen is accelerated, the soluble collagen is directly reduced, the mucopolysaccharide with good water storage property among cells is reduced, the elasticity of connective tissues is low, and the skin loses elasticity, becomes thin and ages. The phenomena of dermal fiber rupture, lipoatrophy, decreased secretion of sweat and sebaceous glands, skin dryness, increased wrinkles, mottling, etc., are all manifestations of "aging", which is continuously exacerbated with age.
In order to supplement collagen lost from skin, some people select foods with high collagen content, such as pig trotters, beef tendons, chicken feet and the like, but the collagen in the foods has high molecular weight, cannot be absorbed and utilized by the skin after being used, and the fat in the foods can make people fat after being eaten too much. Some medical and cosmetic institutions supplement collagen by injection, although some effects, the supplement of collagen by injection has the following defects: on one hand, the process is strong in pain and risks of infection; on the other hand, a special mechanism is needed for injection, and the use cost is high. Therefore, it is very important to develop a cosmetic for improving skin wrinkles and skin firmness by efficiently and conveniently supplementing collagen.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention aims to provide the tightening and wrinkle-removing essence, which can effectively reduce wrinkles on the surface of skin and increase the tightness of the skin.
The invention also aims to provide a preparation method of the firming and wrinkle-removing essence.
One of the purposes of the invention is realized by adopting the following technical scheme:
the tightening and wrinkle-removing essence comprises the following raw materials in parts by weight: 3-7 parts of punicalagin, 1-5 parts of bearberry leaf polyphenol, 1-5 parts of cola nut extract, 10-15 parts of collagen peptide, 5-10 parts of acetyl dipeptide-1 cetyl palmitate, 5-10 parts of ethylhexyl palmitate, 1-3 parts of squalane, 1-3 parts of butanediol, 1-5 parts of emulsifier, 1-5 parts of thickener and 50-60 parts of deionized water;
the collagen peptide is derived from fish scales, and the molecular weight of the collagen peptide is 1000-2000 Da.
Further, the emulsifier is composed of caprylyl glycol, PEG-40 glyceryl cocoate and sodium coco-alcohol polyether sulfate in a weight ratio of 1:1: 2.
Further, the thickening agent is at least one of carbomer, xanthan gum and poloxamer 184.
The second purpose of the invention is realized by adopting the following technical scheme:
the preparation method of the firming and wrinkle-removing essence comprises the following steps:
(1) uniformly stirring and mixing a thickening agent, acetyl dipeptide-1 cetyl ester and butanediol to obtain a component A;
(2) uniformly mixing punicalagin, arbutin polyphenol, cola nut extract, collagen peptide, emulsifier and deionized water to obtain a component B;
(3) and mixing the component A and the component B, adding ethylhexyl palmitate and squalane, homogenizing, naturally cooling, and discharging to obtain the product.
Further, in the step (1), stirring and mixing are carried out for 20-30min at 40-50 ℃.
Further, in the step (2), stirring and mixing are carried out for 10-15min at 70-75 ℃.
Further, the homogenization treatment in the step (3) is carried out at 60-65 ℃ for 10-15 min.
Compared with the prior art, the invention has the beneficial effects that:
1. the punicalagin, the bearberry leaf polyphenol and the cola nut extract are added into the firming and wrinkle-removing essence disclosed by the invention, wherein the punicalagin can promote the proliferation of fibroblasts, the bearberry leaf polyphenol and the cola nut extract can improve the activity of fibroblasts in skin and improve the capability of the fibroblasts in synthesizing collagen, and meanwhile, the essence disclosed by the invention is also added with collagen peptide, so that exogenous supplementary collagen and endogenous collagen synthesis promotion are combined, the content of collagen in the skin is further increased, the skin is firmer, the wrinkle volume on the surface of the skin is reduced, the wrinkle depth is reduced, the cola nut extract also has better corrosion resistance, no preservative is required to be added into the prepared essence, and the essence is safe and non-irritant to the skin. .
2. The essence disclosed by the invention is also added with acetyl dipeptide-1 cetyl ester, ethylhexyl palmitate and squalane, wherein the acetyl dipeptide-1 cetyl ester and the ethylhexyl palmitate are beneficial to promoting collagen peptides to penetrate through a stratum corneum and enter a basal layer, the squalane and squalene in sebum have similar structures and are easily accepted by the skin, the skin affinity is good, and the three components have synergistic effects, so that the absorption and utilization rate of the skin on exogenous collagen peptides is improved.
Detailed Description
The present invention is further described below with reference to specific embodiments, and it should be noted that, without conflict, any combination between the embodiments or technical features described below may form a new embodiment.
Example 1
The tightening and wrinkle-removing essence consists of the following raw materials in parts by weight: 3 parts of punicalagin, 1 part of bearberry leaf polyphenol, 1 part of cola nut extract, 10 parts of collagen peptide, 5 parts of acetyl dipeptide-1 cetyl palmitate, 5 parts of ethylhexyl palmitate, 1 part of squalane, 1 part of butanediol, 0.25 part of caprylyl glycol, 0.25 part of PEG-40 glyceryl cocoate, 0.5 part of sodium cocoeth sulfate, 1 part of carbomer and 50 parts of deionized water;
the collagen peptide is derived from fish scales, and has a molecular weight of 1000 Da.
The preparation method of the firming and wrinkle-removing essence comprises the following steps:
(1) mixing carbomer, acetyl dipeptide-1 cetyl ester and butanediol at 40 deg.C for 20min to obtain component A;
(2) mixing punicalagin, folium Vaccinii Vitis-idaeae polyphenol, cola nut extract, collagen peptide, caprylyl glycol, PEG-40 glyceryl cocoate, sodium cocoyl alcohol polyether sulfate and deionized water at 70 deg.C under stirring for 10min to obtain component B;
(3) mixing the component A and the component B, adding ethylhexyl palmitate and squalane, homogenizing at 60 deg.C for 10min, naturally cooling, and discharging to obtain the final product.
Example 2
The tightening and wrinkle-removing essence consists of the following raw materials in parts by weight: 5 parts of punicalagin, 3 parts of bearberry leaf polyphenol, 2 parts of cola nut extract, 12 parts of collagen peptide, 8 parts of acetyl dipeptide-1 cetyl palmitate, 7 parts of ethylhexyl palmitate, 2 parts of squalane, 2 parts of butanediol, 1 part of octyl glycol, 1 part of PEG-40 glyceryl cocoate, 2 parts of sodium coco-alcohol polyether sulfate, 1 part of xanthan gum, 1842 parts of poloxamer 1842 parts of deionized water;
the collagen peptide is derived from fish scales, and has a molecular weight of 1500 Da.
The preparation method of the firming and wrinkle-removing essence comprises the following steps:
(1) mixing xanthan gum, poloxamer 184, acetyl dipeptide-1 cetyl ester, and butanediol at 45 deg.C under stirring for 20-30min to obtain component A;
(2) mixing punicalagin, folium Vaccinii Vitis-idaeae polyphenol, cola nut extract, collagen peptide, caprylyl glycol, PEG-40 glyceryl cocoate, sodium cocoyl alcohol polyether sulfate and deionized water at 73 deg.C under stirring for 12min to obtain component B;
(3) mixing the component A and the component B, adding ethylhexyl palmitate and squalane, homogenizing at 62 deg.C for 13min, naturally cooling, and discharging to obtain the final product.
Example 3
The tightening and wrinkle-removing essence consists of the following raw materials in parts by weight: 7 parts of punicalagin, 5 parts of bearberry leaf polyphenol, 5 parts of cola nut extract, 15 parts of collagen peptide, 10 parts of acetyl dipeptide-1 cetyl palmitate, 10 parts of ethylhexyl palmitate, 3 parts of squalane, 3 parts of butanediol, 1.25 parts of octyl glycol, 1.25 parts of PEG-40 glyceryl cocoate, 2.5 parts of sodium coco-alcohol polyether sulfate, 1845 parts of poloxamer and 60 parts of deionized water;
the collagen peptide is derived from fish scales, and has a molecular weight of 2000 Da.
The preparation method of the firming and wrinkle-removing essence comprises the following steps:
(1) taking poloxamer 184, acetyl dipeptide-1 cetyl ester and butanediol, stirring and mixing at 50 deg.C for 20-30min to obtain component A;
(2) mixing punicalagin, folium Vaccinii Vitis-idaeae polyphenol, cola nut extract, collagen peptide, caprylyl glycol, PEG-40 glyceryl cocoate, sodium cocoyl alcohol polyether sulfate and deionized water at 75 deg.C under stirring for 15min to obtain component B;
(3) mixing the component A and the component B, adding ethylhexyl palmitate and squalane, homogenizing at 65 ℃ for 15min, naturally cooling, and discharging to obtain the product.
Comparative example 1
Comparative example 1 provides a firming and wrinkle-removing essence, which is different from example 1 in that punicalagin is omitted and the rest is the same as example 1.
Comparative example 2
Comparative example 2 provides a firming and wrinkle-removing essence, which is different from example 1 in that polyphenol of bearberry leaves is omitted, and the rest is the same as example 1.
Comparative example 3
Comparative example 3 provides a firming and wrinkle-removing essence, which is different from example 1 in that the kola nut extract is omitted, and the rest is the same as example 1.
Comparative example 4
Comparative example 4 provides a firming and wrinkle-removing essence, which is different from example 1 in that the collagen peptide is omitted, and the rest is the same as example 1.
Comparative example 5
Comparative example 5 provides a firming and wrinkle-removing essence, which is different from example 1 in that acetyl dipeptide-1 cetyl ester is omitted, the amount of ethylhexyl palmitate is adjusted to 10 parts, and the rest is the same as example 1.
Comparative example 6
Comparative example 6 provides a firming and wrinkle-removing essence, which is different from example 1 in that ethylhexyl palmitate is omitted and the amount of acetyl dipeptide-1 cetyl ester is adjusted to 10 parts, and the rest is the same as example 1.
Comparative example 7
Comparative example 7 provides a firming and wrinkle-removing essence, which is different from example 1 in that squalane is omitted, and the rest is the same as example 1.
Test examples
Selecting 24 volunteers, wherein the ages of the volunteers are 30-40 years, the average age is 35.6 years, the male and female accounts for half, randomly dividing the volunteers into three groups, and each group comprises 8 people, placing the essence (about 0.02-0.025g) of the embodiments 1-3 into a spot tester by a closed spot test method, applying the essence to the inner side of the upper arm of the volunteer by using a medical adhesive tape, removing the essence after 24 hours, observing skin reactions after 0.5, 24 and 48 hours after the spot is removed, and recording the results according to the skin reaction grading standard in technical Specification for cosmetic safety (2015 edition). The specific reaction degree grades are shown in table 1, and the experimental results are shown in table 2.
TABLE 1
TABLE 2
Grade | 0(/ human) | 1(/ human) | 2(/ human) | 3(/ man) | 4(/ human) |
Example 1 | 8 | 0 | 0 | 0 | 0 |
Example 2 | 8 | 0 | 0 | 0 | 0 |
Example 3 | 8 | 0 | 0 | 0 | 0 |
As shown in Table 2, the volunteers all have negative reactions, which indicates that the essence of the invention does not cause adverse reactions of skin, is mild and non-irritant to the skin, and is safe to use.
64 female volunteers with the age of 30-40 and the average age of 34.6 years are selected and averagely divided into 8 groups of 8 persons, the product is applied to the canthus of the volunteer once a day in the morning and at night for 4 weeks, and other skin care products are not used in the period. The test was performed before (week 0) and after week 4, respectively. The face of a volunteer is cleaned before detection, the volunteer sits statically for 30min at a constant temperature and humidity, the skin roughness (SR, which is the difference between the maximum gray peak and the minimum gray peak on a straight line in all sections of the skin and the parameter is the maximum height of the unevenness of the skin surface) of the canthus position of the volunteer is tested by using a Visioscan VC20plus of a skin wrinkle tester Courage + Khazaka, skin average roughness (SAR, the arithmetic mean value of the contour of the skin hills above and below a base line of a target skin, namely the arithmetic mean value of the contour of the skin on the limit section is divided into 5 parts according to the length limit, the difference between the contour of the upper hills on the base line and the contour of the lower hills on the base line of each section, namely the skin roughness, is then the roughness average in the length base line is obtained, the skin roughness has a close relation with the number, the thickness and the depth of the skin wrinkles), the same part, the average values were obtained, and the change rates of skin roughness and skin roughness average after week 4 were calculated as shown in table 1, wherein the change rates were calculated by: (mean of test data at week 4-mean of test data at week 0)/mean of test data at week 0 × 100%.
TABLE 3
Sample (I) | Rate of change of skin roughness/%) | Percent change of average roughness of skin% |
Example 1 | -38.78 | -37.42 |
Comparative example 1 | -22.10 | -23.75 |
Comparative example 2 | -25.45 | -24.71 |
Comparative example 3 | -23.21 | -24.05 |
Comparative example 4 | -25.17 | -23.98 |
Comparative example 5 | -28.14 | -27.47 |
Comparative example 6 | -27.95 | -28.36 |
Comparative example 7 | -29.15 | -30.64 |
As can be seen from table 3, the skin roughness and the average skin roughness of the volunteers who applied the essence of example 1 were significantly reduced after using the product for four months, indicating that wrinkles on the skin surface were reduced, the firmness of the skin was increased, and the skin improvement effect was superior to those of comparative examples 1 to 7. Although the essence prepared by omitting punicalagin, bearberry leaf polyphenol, cola nut extract and collagen peptide in comparative examples 1 to 4 has a certain effect on wrinkle improvement, the essence is generally inferior to that of example 1, and the essence is proved to have synergistic effect by adding the three components, promote the proliferation of fibroblasts, improve the collagen synthesis capacity of the fibroblasts, combine with the collagen peptide in the raw materials, combine exogenous collagen supplementation with endogenous collagen synthesis promotion, further increase the collagen content in the skin, enable the skin to be firmer, reduce the wrinkle volume on the surface of the skin, lighten the wrinkle depth and reduce the skin roughness.
The dosage of acetyl dipeptide-1 cetyl ester and ethylhexyl palmitate is adjusted in comparative examples 5 to 6, one of the acetyl dipeptide-1 cetyl ester and the ethylhexyl palmitate is omitted, the dosage of the other component is increased, the squalane is omitted in comparative example 7, the wrinkle removing effect of the prepared essence is different from that of example 1, the addition of the acetyl dipeptide-1 cetyl ester and the ethylhexyl palmitate is beneficial to promoting collagen peptides to penetrate through the stratum corneum and enter the basal layer, the squalene in the squalane and the sebum has similar structures and is easily accepted by the skin and has good skin affinity, the three components have synergistic effect, the absorption and utilization rate of the skin to exogenous collagen peptides is improved, the content of collagen in the skin is increased, and the wrinkle removing effect is realized.
The above embodiments are only preferred embodiments of the present invention, and the protection scope of the present invention is not limited thereby, and any insubstantial changes and substitutions made by those skilled in the art based on the present invention are within the protection scope of the present invention.
Claims (7)
1. The tightening and wrinkle-removing essence is characterized by comprising the following raw materials in parts by weight: 3-7 parts of punicalagin, 1-5 parts of bearberry leaf polyphenol, 1-5 parts of cola nut extract, 10-15 parts of collagen peptide, 5-10 parts of acetyl dipeptide-1 cetyl palmitate, 5-10 parts of ethylhexyl palmitate, 1-3 parts of squalane, 1-3 parts of butanediol, 1-5 parts of emulsifier, 1-5 parts of thickener and 50-60 parts of deionized water;
the collagen peptide is derived from fish scales, and the molecular weight of the collagen peptide is 1000-2000 Da.
2. The tightening and wrinkle-removing essence according to claim 1, wherein the emulsifier is caprylyl glycol, PEG-40 glyceryl cocoate, sodium cocoeth sulfate in a ratio of 1:1:2 by weight.
3. The tightening and wrinkle-removing essence according to claim 1, wherein the thickener is at least one of carbomer, xanthan gum and poloxamer 184.
4. The process for preparing the firming and wrinkle-removing essence as claimed in any one of claims 1 to 3, comprising the steps of:
(1) uniformly stirring and mixing a thickening agent, acetyl dipeptide-1 cetyl ester and butanediol to obtain a component A;
(2) uniformly mixing punicalagin, arbutin polyphenol, cola nut extract, collagen peptide, emulsifier and deionized water to obtain a component B;
(3) and mixing the component A and the component B, adding ethylhexyl palmitate and squalane, homogenizing, naturally cooling, and discharging to obtain the product.
5. The preparation method of the tightening and wrinkle-removing essence according to claim 4, wherein the step (1) is performed by stirring and mixing at 40-50 ℃ for 20-30 min.
6. The preparation method of the tightening and wrinkle-removing essence according to claim 4, wherein the step (2) is performed by stirring and mixing at 70-75 ℃ for 10-15 min.
7. The preparation method of the tightening and wrinkle-removing essence according to claim 4, wherein the homogenization treatment in the step (3) is carried out at 60-65 ℃ for 10-15 min.
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CN112675060A (en) * | 2021-01-06 | 2021-04-20 | 山东华熙海御生物医药有限公司 | Composition and skin care product for resisting skin aging and preparation method thereof |
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Cited By (1)
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CN112675060A (en) * | 2021-01-06 | 2021-04-20 | 山东华熙海御生物医药有限公司 | Composition and skin care product for resisting skin aging and preparation method thereof |
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