CN114246817A

CN114246817A - Wrinkle-removing composition and preparation method and application thereof

Info

Publication number: CN114246817A
Application number: CN202210060301.8A
Authority: CN
Inventors: 王琴; 陈佳纯; 陈乐斌; 肖裕祥
Original assignee: Guangdong Basonnia Biotechnology Co ltd
Current assignee: Guangdong Basonnia Biotechnology Co ltd
Priority date: 2022-01-19
Filing date: 2022-01-19
Publication date: 2022-03-29

Abstract

The invention provides a wrinkle-removing composition and a preparation method and application thereof, wherein the preparation raw materials of the wrinkle-removing composition comprise an efficacy component, a water phase component and an oil phase combination, and the efficacy component comprises acetyl hexapeptide-8, dipeptide diaminobutyryl benzylamide diacetate, anti-wrinkle peptide for tightening face and oat beta-glucan. The wrinkle-removing composition provided by the invention firstly promotes the proliferation of fibroblasts, improves the metabolism of cells, promotes the expression of elastin and improves the softness of skin; the moisture retention and the tightening smoothness of the skin are improved by renewing and repairing the tense skin; finally, the generation of wrinkles is reduced by inhibiting muscle contraction; thereby tightening the eye skin in all directions and lightening the wrinkles of the eyes.

Description

Wrinkle-removing composition and preparation method and application thereof

Technical Field

The invention belongs to the field of cosmetic preparation, and particularly relates to a wrinkle-removing composition as well as a preparation method and application thereof.

Background

The skin is the largest organ of the human body, and the eye skin is the thinnest part of the skin and is very fragile. After the skin is aged or injured by the external environment, the skin of the eyes is dry and thin, and the collagen of the dermis layer is continuously reduced, so that the reticular supporting force is lost, and various eye problems such as wrinkles, fishtail lines and the like appear on the skin. With the continuous improvement of the living standard of people at present, the requirements of people on skin care are also continuously improved, and people pay more and more attention to eye skin care. The existing products on the market achieve the effect of removing wrinkles by mainly adding a film-forming agent, a filling agent, an expression line inhibitor, a collagen fiber repairing agent and the like.

CN105943475A discloses an anti-wrinkle eye cream containing traditional Chinese medicine components and a preparation method and application thereof, wherein the anti-wrinkle eye cream comprises glycerol, methyl glucoside eicosoxy-ethylene, stearic acid, a silicon smoothing agent, hyaluronic acid, isomerized sugar, rose essential oil, an astragalus extract, an acanthopanax extract, a rhodiola rosea extract, a gynostemma pentaphylla extract and a lithospermum extract. The anti-wrinkle eye cream provided by the invention is fresh, cool, smooth, mild and non-irritant, can maintain moisture of eye skin for a long time, promotes blood circulation of eyes, effectively reduces fine wrinkles of the eyes, but cannot repair the generated fine wrinkles.

CN110897898A discloses an anti-wrinkle moisturizing eye patch and a preparation method thereof, wherein the anti-wrinkle moisturizing eye patch comprises eye patch liquid, and the eye patch liquid is composed of 1.5-4.5 parts by weight of ginseng extract, 1-3 parts by weight of retinol, 3-7 parts by weight of resveratrol, 1.5-4.5 parts by weight of jellyfish extract, 1.5-4.5 parts by weight of citrus extract, 1-3 parts by weight of arbutin, 3-7 parts by weight of papain, 0.5-1.5 parts by weight of polypeptide-1, 1-3 parts by weight of trehalose, 3-7 parts by weight of orange extract, 3-7 parts by weight of green tea pearl powder, 1.5-4.5 parts by weight of mint extract, 1-3 parts by weight of cubilose extract, 1-3 parts by weight of scutellaria root extract and 1.5-4.5 parts by weight of propolis. The eye patch liquid of the anti-wrinkle moisturizing eye patch has good permeability and is easy to absorb, and the eye patch is endowed with anti-wrinkle and moisturizing effects; but the anti-wrinkle moisturizing eye mask provided by the invention has slow effect.

Therefore, how to provide a wrinkle-removing composition with good wrinkle-removing effect and quick response becomes a problem to be solved at present.

Disclosure of Invention

Aiming at the defects of the prior art, the invention aims to provide a wrinkle-removing composition and a preparation method and application thereof. The wrinkle-removing composition provided by the invention firstly promotes the proliferation of fibroblasts, improves the metabolism of cells, promotes the expression of elastin and improves the softness of skin; the moisture retention and the tightening smoothness of the skin are improved by renewing and repairing the tense skin; finally, the generation of wrinkles is reduced by inhibiting muscle contraction; thereby tightening the eye skin in all directions and lightening the wrinkles of the eyes.

In order to achieve the purpose, the invention adopts the following technical scheme:

in a first aspect, the invention provides a wrinkle-removing composition prepared from raw materials comprising an efficacy component, an aqueous phase component and an oil phase combination, wherein the efficacy component comprises acetyl hexapeptide-8, dipeptide diaminobutyryl benzylamide diacetate, anti-wrinkle and oat beta-glucan.

In the invention, acetyl hexapeptide-8, dipeptide diaminobutyrylbenzylamide diacetate, tight face wrinkle resistant peptide and oat beta-glucan are mutually matched for synergistic interaction; firstly, the expression of elastin is promoted and the softness of skin is improved by promoting the proliferation of fibroblasts and improving the metabolism of cells; the moisture retention and the tightening smoothness of the skin are improved by renewing and repairing the tense skin; finally, the generation of wrinkles is reduced by inhibiting muscle contraction; thereby tightening the eye skin in all directions and lightening the wrinkles of the eyes.

In the invention, the weight ratio of the combination of the efficacy component, the water phase component and the oil phase is (5-24): (65-93): (4.5-24), and can be, for example, 5:65:4.5, 7:70:6, 9:73:8, 11:75:10, 13:82:12, 15:88:14, 17:90:16, 19:91:18, 21:92:20, 23:93:22, 24:89:24 and the like. Preferably, the efficacy components comprise the following components in parts by weight: acetyl hexapeptide-81-5 parts, dipeptide diaminobutyrylbenzylamide diacetate 1-5 parts, tight face wrinkle resistant peptide 1-4 parts and oat beta-glucan 1-5 parts.

In the present invention, the acetyl hexapeptide-8 is present in an amount of 1 to 5 parts by weight, for example, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, etc.

In the present invention, the dipeptide diaminobutyrylbenzylamide diacetate is 1 to 5 parts by weight, for example, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, and the like.

In the present invention, the weight portion of the tight face anti-wrinkle peptide is 1-4 parts, for example, 1 part, 2 parts, 3 parts, 4 parts, etc.

In the present invention, the oat β -glucan is 1 to 5 parts by weight, and may be 1 part, 2 parts, 3 parts, 4 parts, 5 parts, or the like, for example.

According to the invention, firstly, the anti-wrinkle peptide for tightening the face promotes the proliferation of fibroblasts, improves the cell metabolism, promotes the synthesis of collagen, improves the softness of the skin, repairs the visual signs of aging and provides a long-term skin lifting effect; through the excellent transdermal absorption performance of the oat beta-glucan, the moisture retention of the skin is quickly improved, the roughness is reduced, the synthesis of various collagens is promoted, the tense skin is renewed and repaired, and the moisture retention and the tightening smoothness of the skin are improved; finally, the acetyl hexapeptide-8 and the dipeptide diaminobutyrylbenzylamide diacetate can reduce the generation of wrinkles at different sites by inhibiting muscle contraction; the acetyl hexapeptide-8, the dipeptide diaminobutyryl benzylamide diacetate, the anti-wrinkle tightening peptide and the oat beta-glucan are matched with each other, have synergistic effect, tighten the eye skin in an all-round way and lighten the wrinkles of the eyes.

Preferably, the anti-wrinkle and anti-wrinkle peptide comprises sweet almond seed extract and hydrolyzed rice protein.

Preferably, the weight ratio of the sweet almond seed extract to the hydrolyzed rice protein is (1.5-2.5):1, and may be, for example, 1.5:1, 1.6:1, 1.7:1, 1.8:1, 1.9:1, 2.0:1, 2.1:1, 2.2:1, 2.3:1, 2.4:1, 2.5:1, and the like.

Preferably, the efficacy component further comprises ON-mediline.

Preferably, the ON-mediline is present in 1-5 parts by weight, and may be, for example, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, etc.

Preferably, the ON-mediline comprises centella asiatica extract, polygonum cuspidatum root extract, astragalus membranaceus root extract, tea leaf extract, glycyrrhiza glabra root extract, chamomile flower extract, and rosmarinus officinalis extract.

In the invention, the ON-MEDI LINE is a compound plant anti-allergy agent extracted from seven plants, and can relieve skin and reduce anaphylactic reaction.

Preferably, the weight ratio of the centella asiatica extract, the polygonum cuspidatum root extract, the astragalus membranaceus root extract, the tea leaf extract, the glycyrrhiza glabra root extract, the chamomile flower extract and the rosewood extract is 1 (0.5-1.5): 0.5-1.5: (0.5-1.5), for example, 1:0.5:0.5:0.5:0.5:0.5: 0.7:0.7:0.7:0.7:0.7: 1:1:1:1: 1.1:1.1:1.1:1.1: 1.9: 0.9: 1.1.1: 1.5:1.5:1.5:1.5: 1.7: 1.1.1: 1.1.5: 1.5:1.5: 1.5.

In the present invention, the aqueous phase component includes a humectant, a coffee extract, and water.

Preferably, the water phase component comprises 1-7 parts by weight of humectant, 0.1-1 part by weight of coffee extract and 65-85 parts by weight of water.

In the present invention, the humectant is 1 to 7 parts by weight, and may be, for example, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, or the like.

In the present invention, the coffee extract may be present in an amount of 0.1 to 1 part by weight, for example, 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1 part, etc.

In the present invention, the amount of the water is 65 to 85 parts by weight, and examples thereof include 65 parts, 67 parts, 69 parts, 71 parts, 73 parts, 75 parts, 77 parts, 79 parts, 81 parts, 83 parts, and 85 parts.

Preferably, the humectant comprises any one or a combination of at least two of trehalose, sodium hyaluronate, 1, 2-hexanediol or butanediol, preferably a combination of trehalose, sodium hyaluronate, 1, 2-hexanediol and butanediol.

In the invention, the trehalose, the sodium hyaluronate, the 1, 2-hexanediol and the butanediol are mutually matched, have synergistic interaction, build a three-dimensional retention system of skin and jointly improve the moisturizing performance of the anti-wrinkle composition. Improving the skin state and increasing the firmness and smoothness of the skin.

Preferably, the weight ratio of trehalose, sodium hyaluronate, 1, 2-hexanediol and butanediol is (0.2-2): (0.02-0.2): (0.1-0.5): (1-4), and may be, for example, 0.2:0.02:0.5:1, 0.4:0.04:0.4:1.5, 0.6:0.06:0.3:2, 0.8:0.08:0.2:2.5, 1.0:0.10:0.1:2.9, 1.2:0.12:0.1:3.2, 1.4:0.14:0.1:3.4, 1.6:0.16:0.1:3.6, 1.8:0.18:0.1:3.8, 2.0:0.20:0.1:4, etc.

Preferably, the molecular weight of the sodium hyaluronate is 1000-3000 kDa.

Preferably, the water comprises deionized water.

Preferably, the aqueous phase component further comprises a thickener.

Preferably, the thickener is present in an amount of 0.2 to 0.6 parts by weight, and may be, for example, 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, etc.

Preferably, the thickener comprises any one or a combination of at least two of carbomer, sodium carboxymethylcellulose or xanthan gum, preferably carbomer.

In the present invention, the oil phase component includes an emollient and an emulsifier.

Preferably, the oil phase component comprises 2-13 parts of an emollient and 1-5 parts of an emulsifier in parts by weight.

In the present invention, the weight part of the emollient is 2 to 13 parts, and may be, for example, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, etc.

In the present invention, the emulsifier is 1 to 5 parts by weight, for example, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, etc.

Preferably, the emollient comprises any one or a combination of at least two of dioctyl carbonate, squalane, argan nut oil, or shea butter, preferably a combination of dioctyl carbonate, squalane, argan nut oil, and shea butter.

In the invention, dioctyl carbonate, squalane, argan tree kernel oil and shea butter are matched with each other, so that the synergy is realized, a skin barrier is built, the skin is deeply nourished, and the skin becomes soft and elastic; the permeability is increased, so that various components in the anti-wrinkle composition are more easily absorbed by the skin.

Preferably, the weight ratio of the dioctyl carbonate, the squalane, the argan tree kernel oil and the shea butter is (1-5): (0.1-1): (0.5-2), and can be 1:1:0.1:0.5, 2:2:0.3:1.0, 3:3:0.5:1.3, 4:4:0.7:1.7, 5:5:1:2 and the like.

Preferably, the emulsifier comprises any one or a combination of at least two of Simulsol 165, PolyAquol 2W or TWEEN 80, preferably PolyAquol 2W.

Preferably, the PolyAquol 2W includes polyglyceryl-2 stearate, glyceryl stearate, and stearic acid.

Preferably, the oil phase component further comprises an antioxidant.

Preferably, the antioxidant is present in an amount of 0.1 to 1 part by weight, and may be, for example, 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1 part, etc.

Preferably, the antioxidant comprises any one or a combination of at least two of tocopheryl acetate, tea polyphenols or glutathione, preferably tocopheryl acetate.

In the present invention, tocopheryl acetate can be used in combination with other components to improve the antioxidant capacity of the wrinkle-reducing composition.

In the invention, the raw materials for preparing the wrinkle-removing composition also comprise a pH regulator.

Preferably, the pH adjuster is 0.06-0.25 parts by weight, and may be, for example, 0.06 parts, 0.08 parts, 0.1 parts, 0.12 parts, 0.14 parts, 0.16 parts, 0.19 parts, 0.2 parts, 0.22 parts, 0.25 parts, etc.

Preferably, the pH adjusting agent comprises any one or a combination of at least two of aminomethyl propanol, triethanolamine or isopropanolamine, preferably aminomethyl propanol.

In the present invention, since the thickener carbomer in the aqueous phase component is acidic, a pH adjusting agent is required for neutralization.

Preferably, the raw materials for preparing the wrinkle-removing composition also comprise a chelating agent.

Preferably, the chelating agent comprises caprylhydroxamic acid and/or disodium edetate, preferably caprylhydroxamic acid.

As a preferred technical scheme, the wrinkle-removing composition comprises preparation raw materials, an efficacy component, a water phase component, an oil phase component, a pH regulator and a chelating agent;

wherein the functional components comprise the following components in parts by weight: 1-5 parts of oat beta-glucan, 81-5 parts of acetyl hexapeptide, 1-5 parts of dipeptide diaminobutyryl benzylamide diacetate, 1-4 parts of tight face and anti-wrinkle peptide and 1-5 parts of ON-MEDI LINE;

the water phase components comprise the following components in parts by weight: 0.2-2 parts of trehalose, 0.02-0.1 part of sodium hyaluronate, 0.1-0.5 part of 1, 2-hexanediol, 1-4 parts of butanediol, 0.1-1 part of coffee extract, 0.2-0.6 part of carbomer and 65-85 parts of deionized water;

the oil phase comprises the following components in parts by weight: 1-5 parts of dioctyl carbonate, 1-5 parts of squalane, 0.1-1 part of argania spinosa kernel oil, 0.5-2 parts of shea butter, 1-5 parts of Polyaquol 2W and 0.1-1 part of tocopherol acetate;

the pH regulator comprises the following components in parts by weight: 0.06-0.25 part of aminomethyl propanol, wherein the chelating agent comprises the following components in parts by weight: 0.01-0.3 part of caprylyl hydroximic acid.

As a further preferable technical scheme, the wrinkle-removing composition comprises preparation raw materials, an efficacy component, a water phase component, an oil phase component, a pH regulator and a chelating agent;

wherein the functional components comprise the following components in parts by weight: 2-3 parts of oat beta-glucan, 82-4 parts of acetyl hexapeptide, 1-2 parts of dipeptide diaminobutyryl benzylamide diacetate, 1-2 parts of tight face and anti-wrinkle peptide and 1-2 parts of ON-MEDI LINE;

the water phase components comprise the following components in parts by weight: 0.5-1.5 parts of trehalose, 0.02-0.05 part of sodium hyaluronate, 0.3-0.5 part of 1, 2-hexanediol, 1.5-4 parts of butanediol, 0.1-0.5 part of coffee extract, 0.3-0.5 part of carbomer and 68-83 parts of deionized water;

the oil phase comprises the following components in parts by weight: 2-4 parts of dioctyl carbonate, 1-3 parts of squalane, 0.3-1 part of argania spinosa kernel oil, 0.8-2 parts of shea butter, 1-3 parts of Polyaquol 2W and 0.2-1 part of tocopherol acetate;

the pH regulator comprises the following components in parts by weight: 0.15-0.2 part of aminomethyl propanol, wherein the chelating agent comprises the following components in parts by weight: 0.01-0.2 part of caprylyl hydroximic acid.

In a second aspect, the present invention provides a method of preparing a wrinkle-reducing composition as defined in the first aspect, the method comprising the steps of: and mixing the functional components containing acetyl hexapeptide-8, dipeptide diaminobutyrylbenzyl amide diacetate and face-tightening anti-wrinkle peptide, the water phase component and the oil phase component, and stirring to obtain the wrinkle-removing composition.

Preferably, the preparation method of the aqueous phase component comprises the following steps: mixing humectant, coffee extract, optional thickener and deionized water, and stirring to obtain water phase component; the humectant comprises any one or a combination of at least two of trehalose, sodium hyaluronate or butanediol.

Preferably, in the preparation method of the aqueous phase component, the stirring temperature is 75-85 deg.C, such as 75 deg.C, 76 deg.C, 77 deg.C, 78 deg.C, 79 deg.C, 80 deg.C, 81 deg.C, 82 deg.C, 83 deg.C, 84 deg.C, 85; the time is 10-15min, such as 10min, 11min, 12min, 13min, 14min, 15min, etc.

Preferably, the preparation method of the oil phase component comprises the following steps: mixing emollient, emulsifier and optional antioxidant, and stirring to obtain the oil phase component.

Preferably, in the preparation method of the oil phase component, the stirring temperature is 75-85 deg.C, such as 75 deg.C, 76 deg.C, 77 deg.C, 78 deg.C, 79 deg.C, 80 deg.C, 81 deg.C, 82 deg.C, 83 deg.C, 84 deg.C, 85; the time is 10-15min, such as 10min, 11min, 12min, 13min, 14min, 15min, etc.

In the present invention, the wrinkle-removing composition is prepared by a method comprising the steps of:

(a) mixing the water phase component and the oil phase component, stirring and homogenizing to obtain a mixture 1;

(b) mixing a pH regulator with the mixture 1 obtained in the step (a), and stirring to obtain a mixture 2;

(c) mixing a chelating agent, optionally 1, 2-hexanediol, and the mixture 2 obtained in step (b), and stirring to obtain a mixture 3;

(d) mixing the functional components with the mixture 3 obtained in the step (c), and stirring to obtain the wrinkle-removing composition.

Preferably, in step (a), the stirring temperature is 75-85 ℃, for example, 75 ℃, 76 ℃, 77 ℃, 78 ℃, 79 ℃, 80 ℃, 81 ℃, 82 ℃, 83 ℃, 84 ℃, 85; the time is 5-10min, such as 5min, 6min, 7min, 8min, 9min, 10min, etc.

Preferably, in step (b), the stirring temperature is 60-65 ℃, for example, 60 ℃, 61 ℃, 62 ℃, 63 ℃, 64 ℃, 65 ℃ and the like; the time is 5-10min, such as 5min, 6min, 7min, 8min, 9min, 10min, etc.

Preferably, in step (c), the stirring temperature is 50-55 deg.C, such as 50 deg.C, 51 deg.C, 52 deg.C, 53 deg.C, 54 deg.C, 55 deg.C; the time is 5-10min, such as 5min, 6min, 7min, 8min, 9min, 10min, etc.

Preferably, in the step (d), the stirring temperature is 25-40 ℃, for example, 25 ℃, 27 ℃, 29 ℃, 31 ℃, 33 ℃, 35 ℃, 37 ℃, 40 ℃ and the like; the time is 10-15min, such as 10min, 11min, 12min, 13min, 14min, 15min, etc.

As a preferred technical scheme of the invention, the preparation method of the wrinkle-removing composition comprises the following steps:

(1) mixing humectant, coffee extract, optional thickener and deionized water, stirring at 75-85 deg.C for 10-15min to obtain water phase component, wherein the humectant comprises one or more of trehalose, sodium hyaluronate or butanediol; mixing emollient, emulsifier and optional antioxidant, and stirring at 75-85 deg.C for 10-15min to obtain oil phase component;

(2) mixing the water phase component and the oil phase component obtained in the step (1), stirring for 5-10min at 75-85 ℃, and homogenizing to obtain a mixture 1;

(3) mixing a pH regulator with the mixture 1 obtained in the step (2), and stirring at 60-65 ℃ for 5-10min to obtain a mixture 2;

(4) mixing a chelating agent, optional 1, 2-hexanediol and the mixture 2 obtained in the step (3), and stirring at 50-55 ℃ for 5-10min to obtain a mixture 3;

(5) and (4) mixing the functional components with the mixture 3 obtained in the step (4), and stirring for 10-15min at 25-40 ℃ to obtain the wrinkle-removing composition.

In a third aspect, the present invention provides a use of the wrinkle-removing composition according to the first aspect in the preparation of a cosmetic for wrinkle-removing and tightening eyes.

Compared with the prior art, the invention has the following beneficial effects:

(1) the wrinkle-removing composition provided by the invention firstly promotes the proliferation of fibroblasts, improves the metabolism of cells, promotes the expression of elastin and improves the softness of skin; the moisture retention and the tightening smoothness of the skin are improved by renewing and repairing the tense skin; finally, the generation of wrinkles is reduced by inhibiting muscle contraction; therefore, the eye skin can be tightened in all directions, and the wrinkles of the eyes can be reduced;

(2) the wrinkle-removing composition provided by the invention can increase the elasticity of the skin by supplementing the moisture of the skin and constructing a moisture loss barrier, so that the skin is more young, and the effects of supplementing water and resisting wrinkles are achieved;

(3) the wrinkle-removing composition provided by the invention has a good free radical removing effect, and the excellent oxidation resistance can reduce the generation of static wrinkles.

Detailed Description

The technical solution of the present invention is further explained by the following embodiments. It should be understood by those skilled in the art that the examples are only for the understanding of the present invention and should not be construed as the specific limitations of the present invention.

The component sources in the following examples are as follows:

example 1

The embodiment provides a wrinkle-removing composition, which comprises the following raw materials in parts by weight:

the preparation method of the wrinkle-removing composition comprises the following steps:

(1) putting deionized water into a water bath, slowly adding carbomer, trehalose, sodium hyaluronate, butanediol and coffee extract, and stirring at 80 deg.C for 15min to obtain water phase component; sequentially adding PolyAquol 2W, dioctyl carbonate, squalane, Aralia mandshurica kernel oil, shea butter and tocopherol acetate into an oil pan, and stirring for 15min at 80 ℃ to obtain the oil phase component;

(2) mixing the water phase component and the oil phase component obtained in the step (1), stirring for 5min at 80 ℃, and homogenizing to obtain a mixture 1;

(3) cooling the mixture 1 obtained in the step (2) to 63 ℃, mixing the aminomethyl propanol with the mixture 1, and stirring at 63 ℃ for 8min to obtain a mixture 2;

(4) cooling the mixture 2 obtained in the step (3) to 53 ℃, mixing the caprylyl hydroximic acid, the 1, 2-hexanediol and the mixture 2, and stirring for 8min at 53 ℃ to obtain a mixture 3;

(5) cooling mixture 3 to 35 deg.C, mixing oat beta-glucan, fobotox, snawring, tightening anti-wrinkle peptide, ON-MEDI LINE and mixture 3, and stirring at 35 deg.C for 15min to obtain the wrinkle-removing composition.

Example 2

(1) putting deionized water into a water bath kettle, slowly adding carbomer, trehalose, sodium hyaluronate, butanediol and coffee extract, and stirring at 81 deg.C for 15min to obtain water phase component; sequentially adding PolyAquol 2W, dioctyl carbonate, squalane, Aralia mandshurica kernel oil, shea butter and tocopherol acetate into an oil pan, and stirring for 15min at 81 ℃ to obtain the oil phase component;

(2) mixing the water phase component and the oil phase component obtained in the step (1), stirring for 5min at 81 ℃, and homogenizing to obtain a mixture 1;

(3) cooling the mixture 1 obtained in the step (2) to 64 ℃, mixing the aminomethyl propanol with the mixture 1, and stirring at 64 ℃ for 5min to obtain a mixture 2;

(4) cooling the mixture 2 obtained in the step (3) to 54 ℃, mixing the caprylyl hydroximic acid, the 1, 2-hexanediol and the mixture 2, and stirring for 5min at 54 ℃ to obtain a mixture 3;

(5) cooling mixture 3 to 37 deg.C, mixing oat beta-glucan, fobotox, snawring, tight-face anti-wrinkle peptide, ON-MEDI LINE and mixture 3, and stirring at 37 deg.C for 15min to obtain the anti-wrinkle composition.

Example 3

(1) putting deionized water into a water bath, slowly adding carbomer, trehalose, sodium hyaluronate, butanediol and coffee extract, and stirring at 82 deg.C for 15min to obtain water phase component; sequentially adding PolyAquol 2W, dioctyl carbonate, squalane, Aralia mandshurica kernel oil, shea butter and tocopherol acetate into an oil pan, and stirring for 15min at 82 ℃ to obtain the oil phase component;

(2) mixing the water phase component and the oil phase component obtained in the step (1), stirring for 5min at 82 ℃, and homogenizing to obtain a mixture 1;

(3) cooling the mixture 1 obtained in the step (2) to 62 ℃, mixing the aminomethyl propanol with the mixture 1, and stirring at 62 ℃ for 8min to obtain a mixture 2;

(4) cooling the mixture 2 obtained in the step (3) to 52 ℃, mixing the caprylhydroxamic acid and the 1, 2-hexanediol with the mixture 2, and stirring for 9min at 52 ℃ to obtain a mixture 3;

(5) cooling the mixture 3 to 30 deg.C, mixing oat beta-glucan, fobotox, snawring, tight-face anti-wrinkle peptide, ON-MEDI LINE and the mixture 3, and stirring at 30 deg.C for 13min to obtain the anti-wrinkle composition.

Example 4

This example provides a wrinkle-reducing composition differing from example 1 only in the absence of ON-mediline, the other components and the method of preparation being the same as in example 1.

Examples 5 to 8

Examples 5-8 provide 4 wrinkle-removing compositions differing from example 1 only in the absence of any of trehalose, sodium hyaluronate, butylene glycol or 1, 2-hexanediol, respectively, and the other components and methods of preparation are the same as example 1.

Example 9

This example provides 1 wrinkle-removing composition, differing from example 1 only in the absence of coffee extract, other components and preparation method as in example 1.

Examples 10 to 13

Examples 10-13 provide 4 wrinkle-removing compositions differing from example 1 only in the absence of any of dioctyl carbonate, squalane, argan nut oil or shea butter, the other components and methods of preparation being the same as in example 1.

Comparative examples 1 to 4

Comparative examples 1-4 provide 4 wrinkle-removing compositions differing from example 1 only in the absence of oat β -glucan, acetyl hexapeptide-8, dipeptide diaminobutyrylbenzylamide diacetate, anti-wrinkle peptide, or an illustration of any one thereof, respectively, and other components and methods of preparation the same as example 1.

Test example 1

Eye skin stratum corneum water content test

Testing a sample: wrinkle-removing compositions provided in examples 1 to 13 and comparative examples 1 to 4

510 skin-healthy 18-40-year-old volunteers, half-in-proportion each, were selected and randomly divided into 17 groups of 15 men and 15 women, and the wrinkle-removing compositions provided in application examples 1 to 13 and comparative examples 1 to 4 were used, respectively. After cleaning the face, the volunteers apply the wrinkle-removing composition on the eye skin, continuously use for 30 days, and measure the moisture content of the stratum corneum before and after use by a skin tester (MC750, CK in Germany);

the water content of the eye skin cuticle of each subject is respectively tested before use and after 30 days of continuous use, each data is tested for three times, after an average value is obtained, the average value of each group is calculated, one digit after a decimal point is reserved, and the change rate of the water content of the skin is calculated according to the following formula:

eye skin stratum corneum moisture change rate (eye skin stratum corneum moisture content 30 days after use-eye skin stratum corneum moisture content before use)/eye skin stratum corneum moisture content before use;

the test results are shown in table 1:

TABLE 1

As can be seen from the data in table 1, the skin moisture content change rate is 5.1-10.2% after 4 hours using the wrinkle-removing composition provided by the present invention (examples 1-13), and 9.2-10.2% after 4 hours using the wrinkle-removing composition provided by the preferred embodiment of the present invention (examples 1-3).

The stratum corneum of the skin contains two types of water, namely bound water and free water, the water content can influence the formation of water and a grease film in the stratum corneum, and the grease film can prevent the excessive evaporation of the water and soften the skin, and has important significance for resisting aging and removing wrinkles of the skin. As can be seen from Table 1, the water content of the horny layer of the eye skin is obviously improved by using the wrinkle-removing composition provided by the invention, and the effect is superior to that of comparative examples 1-4, which shows that the water content of the eye skin can be synergistically improved and the water loss of the skin can be reduced by adjusting the proportion of each component through the compound use of each component.

As can be seen from a comparison of example 1 and example 4, the moisturizing performance of the anti-wrinkle composition is slightly lower in the absence of ON-MEDI LINE, which is an extract of seven plants, in combination with other components, and improves the skin condition.

As can be seen from the comparison between example 1 and examples 5-8, trehalose, sodium hyaluronate, butanediol and 1, 2-hexanediol are matched with each other to achieve synergistic interaction, so that a three-dimensional retention system of skin is built, and the moisturizing performance of the anti-wrinkle composition is improved. The three components are matched with each other, so that the moisture can be supplemented in time, and the loss of the moisture can be reduced.

As can be seen by comparing example 1 with example 9, the absence of coffee extract reduces the moisturizing properties of the wrinkle-removing composition.

As can be seen from the comparison between example 1 and examples 10 to 13, dioctyl carbonate, squalane, argan nut oil and shea butter cooperate with each other to synergistically enhance the effects of building a skin barrier and deeply nourishing the skin, so that the skin becomes soft and elastic; the permeability is increased, so that various components in the anti-wrinkle composition are more easily absorbed by the skin.

The comparison between the example 1 and the comparative examples 1 to 4 shows that the lack of any one of acetyl hexapeptide-8, dipeptide diaminobutyryl benzylamide diacetate, tight wrinkle resistant peptide or oat beta-glucan can reduce the moisturizing effect of the anti-wrinkle composition, and the acetyl hexapeptide-8, the dipeptide diaminobutyryl benzylamide diacetate, the tight wrinkle resistant peptide and the oat beta-glucan are matched with each other to realize synergistic effect, so that the skin moisture can be supplemented, the protective effect is enhanced from the inner skin bottom layer, the moisture loss barrier is effectively improved, the skin elasticity is increased, the skin is younger, and the moisturizing and anti-wrinkle effect is achieved.

Test example 2

Ocular skin elasticity test

The test method comprises the following steps: selecting 510 volunteers with skin health of 18-40 years old, wherein the proportion of men and women accounts for half respectively, randomly dividing into 17 groups, each group comprises 15 men and 15 women, cleaning the face of the volunteer in the morning and evening each day, smearing wrinkle-removing composition on the skin of the eyes, continuously using for 3 months, and measuring the elasticity of the skin of the eyes by a skin tester (MC750, Germany CK); each data is tested for three times, after the average value is obtained, the average value of each group is calculated, two digits after the decimal point are reserved, and the skin elasticity change rate is calculated according to the following formula:

skin elasticity variation value-skin elasticity parameter 3 months after use-skin elasticity parameter before use

The test results are shown in table 2:

TABLE 2

As can be seen from the data in Table 2, the wrinkle-removing compositions provided by the present invention (examples 1 to 13) exhibited a change in the skin elasticity parameter of 0.05 to 0.09 after 3 months of use; with the wrinkle-removing composition provided in the preferred embodiment of the present invention (examples 1 to 3), the variation value of the skin elasticity parameter after 3 months of use is 0.08 to 0.09.

The greater the value of the skin elasticity parameter, the better the skin elasticity. As can be seen from Table 2, after the wrinkle-removing eye cream prepared by the invention is used by volunteers, the skin elasticity parameter is obviously improved, and the effect is obviously superior to that of comparative examples 1-4, which shows that the wrinkle-removing eye cream can increase the firmness and elasticity of eye skin and improve the skin elasticity.

As can be seen from a comparison of example 1 and example 4, in the absence of ON-MEDI LINE, the effect of increasing skin elasticity is less.

As can be seen from a comparison of example 1 and examples 5-8, the absence of any of trehalose, sodium hyaluronate, butylene glycol or 1, 2-hexanediol in the moisturizer also reduces elastin expression, impairing the effectiveness of the wrinkle-removing composition; this is because the functional components and the moisturizer can cooperate with each other to improve the skin condition and increase the firmness and smoothness of the skin.

As can be seen from a comparison of example 1 and example 9, the effect of the wrinkle-removing composition is impaired in the absence of the coffee extract.

As can be seen by comparing example 1 with examples 10-13, the absence of any of dioctyl carbonate, squalane, argan oil, or shea butter in the absence of an emollient attenuates the effectiveness of the wrinkle-reducing composition because the benefit component and emollient cooperate to increase the firmness and elasticity of the ocular skin and increase skin elasticity.

As can be seen from a comparison of example 1 and comparative examples 1-4, the effect is poor when any one of acetyl hexapeptide-8, dipeptide diaminobutyrylbenzylamide diacetate, anti-wrinkle peptide or oat beta-glucan is absent; the anti-wrinkle composition provided by the invention is characterized in that acetyl hexapeptide-8, dipeptide diaminobutyrylbenzylamide diacetate, tight face anti-wrinkle peptide and oat beta-glucan are matched with each other to realize synergistic interaction; firstly, the expression of elastin is promoted and the softness of skin is improved by promoting the proliferation of fibroblasts and improving the metabolism of cells; the moisture retention and the tightening smoothness of the skin are improved by renewing and repairing the tense skin; finally, the generation of wrinkles is reduced by inhibiting muscle contraction; thereby tightening the eye skin in all directions and lightening the wrinkles of the eyes.

Test example 3

Skin wrinkle reduction test

The test method comprises the following steps: selecting 510 volunteers with healthy skin and 18-40 years old, wherein the proportion of the volunteers accounts for half of that of men and women, randomly dividing the volunteers into 17 groups, and each group comprises 15 males and 15 females; the eyes of the volunteers have fine lines or wrinkles of certain parts are obvious, and the volunteers apply the wrinkle-removing eye cream prepared by the invention on the skin of the eyes after cleaning the face in the morning and at night every day and continuously use for 6 months. Wrinkle removal test wrinkles in the corners of the eyes of a subject are tested using a multifunctional 3D skin image analyzer (Antera 3D) from Ireland MIRAVEX to obtain a 3D image of the surface structure of the skin in the corners of the eyes, and wrinkle-related data, such as wrinkle depth, volume, area, length, etc., are used to determine, by means of systematic statistical analysis, a parameter of wrinkles in the corners of the eyes, the value ranging from 0 to 100, with a larger value representing fewer wrinkles, finer wrinkles, and shallower wrinkles, with better results.

The testing process comprises the following steps: the tested area is the corner of the face. Prior to testing, subjects uniformly cleansed their face and blotted dry with a dry non-dusting absorbent paper towel. Sit still for 30min in a laboratory at a temperature of 20 + -1 deg.C, 50 + -10% RH. The subject remained relaxed, face exposed, avoiding touch. The laboratory technician tests the subject' S eye and corner wrinkle parameters S1 and then instructs the subject to use the product once in the morning and once in the evening, each time in an amount of about half a coin size, during which time no other skin care product is used. After 6 months, the subject visits again, cleans the face with the facial cleanser, and dries the skin with a dry facial tissue, sits still for 30min in a laboratory at a temperature of 21 ± 1 ℃ and 50 ± 10% RH, and the laboratory technician tests the subject' S canthus wrinkle parameters S2; each data was tested three times, and after averaging, the difference between the average value before use and the average value after 6 months of use of the product was calculated for each group of subjects, and then the wrinkle-removing effect of the product was analyzed using the difference. The rate of change of skin moisture content is calculated according to the following formula:

the change value of the eye corner wrinkles is 6 months after use as the parameter S2 of the eye corner wrinkles and before use as the parameter S1 of the eye corner wrinkles

The test results are shown in table 3:

TABLE 3

The data in table 3 show that the wrinkle-removing composition prepared by the invention has good wrinkle-removing effect, can supplement moisture of skin, increase the firmness and elasticity of eye skin, improve the elasticity of skin, and is beneficial to removing fine lines of eyes, enabling the eye skin to be firm and fine and reducing wrinkles.

In the invention, the efficacy component, the water phase component and the oil phase component are cooperated with each other, thereby achieving good wrinkle removing effect.

As can be seen from comparison of example 1 with comparative examples 1 to 4, the wrinkle-removing effect is poor when any one of acetyl hexapeptide-8, dipeptide diaminobutyrylbenzylamide diacetate, anti-wrinkle peptide or oat beta-glucan is absent; the anti-wrinkle composition provided by the invention is characterized in that acetyl hexapeptide-8, dipeptide diaminobutyrylbenzylamide diacetate, tight face anti-wrinkle peptide and oat beta-glucan are matched with each other to realize synergistic interaction; firstly, the expression of elastin is promoted and the softness of skin is improved by promoting the proliferation of fibroblasts and improving the metabolism of cells; the moisture retention and the tightening smoothness of the skin are improved by renewing and repairing the tense skin; finally, the generation of wrinkles is reduced by inhibiting muscle contraction; thereby tightening skin in all directions and eliminating wrinkles.

Test example 4

Safety test

The test method comprises the following steps: the adverse reaction test of human skin is carried out by referring to the relevant requirements of 'the test of skin spots on human skin' in the 'cosmetic hygiene Specification' (2007 edition). 510 healthy 18-40 year old volunteers were selected, half the proportion of men and women, and randomly divided into 17 groups of 15 men and 15 women, and no other skin care products were used during the test. Selecting a spot tester with an area of 6mm multiplied by 6mm, putting a test object into a small chamber of the spot tester, wherein the dosage is 0.025g, sticking the spot tester with the test object on the back of a subject by using a hypoallergenic adhesive tape, and lightly pressing the spot tester with a palm to uniformly stick the spot tester on the skin for 24 hours. The skin reaction is observed after 30min (after the indentation disappears), 1h, 2h and 24h after the test object spot tester is removed. The observation results were recorded as shown in "Classification Standard Table for adverse skin reactions" in Table 4, and the safety test results are shown in Table 5.

TABLE 4

Grade of rating	Skin reactions
		0	Negative reaction
1	Suspicious reaction, only faint erythema
		2	Erythema, infiltrates, edema, and possibly pimples
3	Erythema, infiltration, edema, pimples, dragons; the reaction can exceed the tested area
		4	Obvious erythema, severe infiltration, edema, and fusional herpes; reaction beyond the variation zone

The test results are shown in table 5:

TABLE 5

As can be seen from the data in Table 5, the anti-wrinkle composition provided by the invention is safe and non-irritant.

The applicant states that the invention is illustrated by the above examples to a wrinkle-removing composition of the invention and its preparation method and use, but the invention is not limited to the above examples, i.e. it does not mean that the invention must be practiced by means of the above examples. It should be understood by those skilled in the art that any modification of the present invention, equivalent substitutions of the raw materials of the product of the present invention, addition of auxiliary components, selection of specific modes, etc., are within the scope and disclosure of the present invention.

Claims

1. The wrinkle-removing composition is characterized by being prepared from an effect component, a water phase component and an oil phase, wherein the effect component comprises acetyl hexapeptide-8, dipeptide diaminobutyryl benzylamide diacetate, tightening anti-wrinkle peptide and oat beta-glucan.

2. The wrinkle-removing composition as claimed in claim 1, wherein the weight ratio of the combination of the efficacy component, the water phase component and the oil phase is (5-24): (65-93): (4.5-24);

preferably, the efficacy components comprise the following components in parts by weight: acetyl hexapeptide-81-5 parts, dipeptide diaminobutyrylbenzylamide diacetate 1-5 parts, tight face wrinkle resistant peptide 1-4 parts and oat beta-glucan 1-5 parts;

preferably, the anti-wrinkle and anti-wrinkle peptide comprises sweet almond seed extract and hydrolyzed rice protein;

preferably, the weight ratio of the sweet almond seed extract to the hydrolyzed rice protein is (1.5-2.5) to 1;

preferably, the efficacy component further comprises ON-mediline;

preferably, the weight part of the ON-MEDI LINE is 1-5 parts;

preferably, the ON-mediline comprises centella asiatica extract, polygonum cuspidatum root extract, astragalus membranaceus root extract, tea leaf extract, glycyrrhiza glabra root extract, chamomile flower extract, and rosmarinus officinalis extract;

preferably, the weight ratio of the centella asiatica extract, the polygonum cuspidatum root extract, the astragalus membranaceus root extract, the tea leaf extract, the glycyrrhiza glabra root extract, the chamomile flower extract and the rosewood extract is 1 (0.5-1.5): 0.5-1.5: (0.5-1.5).

3. The wrinkle-removing composition according to claim 1 or 2, wherein the aqueous phase component comprises a moisturizer, a coffee extract and water;

preferably, the water phase component comprises the following components in parts by weight: 1-7 parts of humectant, 0.1-1 part of coffee extract and 65-85 parts of water;

preferably, the humectant comprises any one or a combination of at least two of trehalose, sodium hyaluronate, 1, 2-hexanediol or butanediol, preferably a combination of trehalose, sodium hyaluronate, 1, 2-hexanediol and butanediol;

preferably, the weight ratio of the trehalose to the sodium hyaluronate to the 1, 2-hexanediol to the butanediol is (0.2-2): 0.02-0.2): 0.1-0.5): 1-4;

preferably, the molecular weight of the sodium hyaluronate is 1000-3000 kDa;

preferably, the water comprises deionized water;

preferably, the aqueous phase component further comprises a thickener;

preferably, the weight part of the thickening agent is 0.2-0.6 part;

4. A wrinkle-reducing composition as claimed in any one of claims 1-3, characterized in that the oil phase components comprise an emollient and an emulsifier;

preferably, the oil phase component comprises the following components in parts by weight: 2-13 parts of an emollient and 1-5 parts of an emulsifier;

preferably, the emollient comprises any one or a combination of at least two of dioctyl carbonate, squalane, argan nut oil, or shea butter, preferably a combination of dioctyl carbonate, squalane, argan nut oil, and shea butter;

preferably, the weight ratio of the dioctyl carbonate, the squalane, the argan tree kernel oil and the shea butter is (1-5): (0.1-1): (0.5-2);

preferably, the emulsifier comprises any one or a combination of at least two of Simulsol 165, PolyAquol 2W or TWEEN 80, preferably PolyAquol 2W;

preferably, the PolyAquol 2W comprises polyglycerol-2 stearate, glyceryl stearate and stearic acid;

preferably, the oil phase component further comprises an antioxidant;

preferably, the antioxidant is 0.1-1 part by weight;

5. The wrinkle-removing composition as claimed in any one of claims 1-4, wherein the raw materials for preparing the wrinkle-removing composition further comprise a pH-adjusting agent;

preferably, the weight portion of the pH regulator is 0.06-0.25;

preferably, the pH adjusting agent comprises any one or a combination of at least two of aminomethyl propanol, triethanolamine or isopropanolamine, preferably aminomethyl propanol;

preferably, the raw materials for preparing the wrinkle-removing composition also comprise a chelating agent;

preferably, the chelating agent is 0.01-0.3 part by weight;

6. The wrinkle-removing composition as claimed in any one of claims 1-5, wherein the wrinkle-removing composition is prepared from raw materials comprising an efficacy component, a water phase component, an oil phase component, a pH regulator and a chelating agent;

7. A method of preparing a wrinkle-reducing composition according to any one of claims 1-6, characterised in that the method of preparation comprises the steps of: mixing and stirring functional components containing acetyl hexapeptide-8, dipeptide diaminobutyrylbenzyl amide diacetate and face-tightening anti-wrinkle peptide, a water phase component and an oil phase component to obtain the wrinkle-removing composition;

preferably, the preparation method of the aqueous phase component comprises the following steps: mixing humectant, coffee extract, optional thickener and deionized water, and stirring to obtain water phase component; the humectant comprises any one or a combination of at least two of trehalose, sodium hyaluronate or butanediol;

preferably, in the preparation method of the water phase component, the stirring temperature is 75-85 ℃ and the time is 10-15 min;

preferably, the preparation method of the oil phase component comprises the following steps: mixing and stirring an emollient, an emulsifier and an optional antioxidant to obtain the oil phase component;

preferably, in the preparation method of the oil phase component, the stirring temperature is 75-85 ℃ and the time is 10-15 min.

8. A method of preparing a wrinkle-reducing composition as claimed in claim 7, characterized in that the method of preparing the wrinkle-reducing composition comprises the steps of:

(d) mixing the functional components with the mixture 3 obtained in the step (c), and stirring to obtain the wrinkle-removing composition;

preferably, in the step (a), the stirring temperature is 75-85 ℃ and the stirring time is 5-10 min;

preferably, in the step (b), the stirring temperature is 60-65 ℃ and the stirring time is 5-10 min;

preferably, in the step (c), the stirring temperature is 50-55 ℃ and the stirring time is 5-10 min;

preferably, in step (d), the stirring temperature is 25-40 ℃ and the time is 10-15 min.

9. A method of preparing a wrinkle-reducing composition as claimed in claim 7 or 8, characterized in that the method comprises the steps of:

10. Use of a wrinkle-removing composition according to any one of claims 1 to 6 for the preparation of a cosmetic for wrinkle-removing and tightening the eyes.