CN110462025B - 增强干细胞存活和增殖的培养基和方法 - Google Patents
增强干细胞存活和增殖的培养基和方法 Download PDFInfo
- Publication number
- CN110462025B CN110462025B CN201880019192.7A CN201880019192A CN110462025B CN 110462025 B CN110462025 B CN 110462025B CN 201880019192 A CN201880019192 A CN 201880019192A CN 110462025 B CN110462025 B CN 110462025B
- Authority
- CN
- China
- Prior art keywords
- acid
- stem cells
- lipid
- cells
- culture medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/36—Lipids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/15—Transforming growth factor beta (TGF-β)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
- C12N2501/72—Transferases [EC 2.]
- C12N2501/727—Kinases (EC 2.7.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/999—Small molecules not provided for elsewhere
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2533/00—Supports or coatings for cell culture, characterised by material
- C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Developmental Biology & Embryology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Gynecology & Obstetrics (AREA)
- Reproductive Health (AREA)
- Transplantation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762449413P | 2017-01-23 | 2017-01-23 | |
| US62/449,413 | 2017-01-23 | ||
| US201762518776P | 2017-06-13 | 2017-06-13 | |
| US62/518,776 | 2017-06-13 | ||
| US201762608875P | 2017-12-21 | 2017-12-21 | |
| US62/608,875 | 2017-12-21 | ||
| PCT/CA2018/050076 WO2018132926A1 (en) | 2017-01-23 | 2018-01-23 | Media and methods for enhancing the survival and proliferation of stem cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110462025A CN110462025A (zh) | 2019-11-15 |
| CN110462025B true CN110462025B (zh) | 2023-11-21 |
Family
ID=62907538
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201880019192.7A Active CN110462025B (zh) | 2017-01-23 | 2018-01-23 | 增强干细胞存活和增殖的培养基和方法 |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US11697796B2 (enExample) |
| EP (2) | EP4361256A3 (enExample) |
| JP (1) | JP7049347B2 (enExample) |
| CN (1) | CN110462025B (enExample) |
| AU (1) | AU2018209127B2 (enExample) |
| CA (1) | CA3051141C (enExample) |
| IL (1) | IL268194B (enExample) |
| SG (1) | SG11201906731XA (enExample) |
| WO (1) | WO2018132926A1 (enExample) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA3051141C (en) | 2017-01-23 | 2021-06-22 | Stemcell Technologies Canada Inc. | Media and methods for enhancing the survival and proliferation of stem cells |
| CN111808797A (zh) * | 2020-07-28 | 2020-10-23 | 湖南赛诺生物科技股份有限公司 | Necrostatin-1的应用及促进新生猪胰岛细胞成熟的制剂 |
| JP2022032793A (ja) * | 2020-08-14 | 2022-02-25 | アルブキュラ コーポレーション | アルブミン組成物並びにそれを製造及び使用する方法 |
| IL300809A (en) | 2020-09-02 | 2023-04-01 | Yztherapeutic Performance Ltd | Cellular preparation for the treatment of diseases and method of use |
| WO2022114724A1 (ko) * | 2020-11-27 | 2022-06-02 | (주)엑셀세라퓨틱스 | 고효율 및 고순도 엑소좀 생산용 배지 조성물 |
| WO2022173058A1 (ja) * | 2021-02-15 | 2022-08-18 | 凸版印刷株式会社 | 三次元組織体の製造方法及び脂肪由来幹細胞の分化促進方法 |
| CN116121186A (zh) * | 2023-01-03 | 2023-05-16 | 内蒙古奥普赛生物科技有限公司 | 一种利用棕榈油酸培养脂肪间充质干细胞的方法 |
| WO2024216399A1 (en) * | 2023-04-20 | 2024-10-24 | Stemcell Technologies Canada Inc. | Methods for large scale expansion of pluripotent stem cells (pscs) |
| CN116814532B (zh) * | 2023-07-10 | 2025-02-11 | 上海迈邦生物科技有限公司 | 一种用于哺乳动物细胞培养基的脂类复合物及其制备方法与应用 |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101052712A (zh) * | 2004-09-08 | 2007-10-10 | 威斯康星校友研究基金会 | 培养人胚胎干细胞 |
| CN101182464A (zh) * | 2006-05-16 | 2008-05-21 | 贝克顿·迪金森公司 | 用于细胞培养的经细胞外基质包被的表面 |
| JP2009542247A (ja) * | 2006-07-12 | 2009-12-03 | ユニヴァーシティー オヴ シェフィールド | 細胞増殖培地 |
| JP2012175962A (ja) * | 2011-01-31 | 2012-09-13 | National Institute Of Biomedical Innovation | ヒト多能性幹細胞の培養方法 |
| CN105283541A (zh) * | 2013-05-30 | 2016-01-27 | 味之素株式会社 | 干细胞培养用培养基 |
| CA2981277A1 (en) * | 2015-03-30 | 2016-10-06 | Ajinomoto Co., Inc. | Human serum albumin-containing culture medium for growth of neural stem cells |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0986635A4 (en) | 1997-01-10 | 2001-11-07 | Life Technologies Inc | SERUM SUBSTITUTE FOR EMBRYONIC STEM CELLS |
| US9074176B2 (en) * | 2006-01-13 | 2015-07-07 | Two Cells Co., Ltd. | Culture medium additive for use in serum-free culturing of animal cell, kit and use thereof |
| ES2360782B1 (es) | 2009-07-28 | 2012-03-12 | Grifols, S.A. | Medios para cultivo de células de mamíferos que comprenden sobrenadante de etapas del fraccionamiento de Cohn y uso de los mismos. |
| FI20096288A0 (fi) * | 2009-12-04 | 2009-12-04 | Kristiina Rajala | Formulations and methods for culturing stem cells |
| WO2011085072A2 (en) | 2010-01-06 | 2011-07-14 | Ervin Industries, Inc. | Frangible, ceramic-metal composite objects and methods of making the same |
| US8497124B2 (en) * | 2011-12-05 | 2013-07-30 | Factor Bioscience Inc. | Methods and products for reprogramming cells to a less differentiated state |
| RU2018128383A (ru) | 2012-03-07 | 2019-03-14 | Янссен Байотек, Инк. | Среда определенного состава для размножения и обновления плюрипотентных стволовых клеток |
| JP2016521971A (ja) * | 2013-04-23 | 2016-07-28 | イェダ リサーチ アンド ディベロップメント カンパニー リミテッド | 単離ナイーブ型多能性幹細胞およびそれを発生させる方法関連出願本出願は、米国特許法119条第(e)項に基づき、2014年1月29日出願の米国特許仮出願第61/932,935号、2013年9月17日出願の米国特許仮出願第61/878,769号、および2013年4月23日出願の米国特許仮出願第61/814,920号の優先権を主張する。また、本出願は、同時に提出された同出願人による同時係属出願である、YaqubHANNA、NoaNOVERSHTERN、およびYoachRAISによる米国特許出願(発明の名称「単離ナイーブ型多能性幹細胞およびそれを発生させる方法(ISOLATEDNAIVEPLURIPOTENTSTEMCELLSANDMETHODSOFGENERATINGSAME)」)(代理人事件記録簿第58870号)にも関する。上記出願の内容はその全体を参考として本明細書に組み込む。 |
| WO2015042356A1 (en) * | 2013-09-19 | 2015-03-26 | The United States Of America, As Represented By The Secretary, Department Of Health & Human Services | Chemically defined culture medium for stem cell maintenance and differentiation |
| CA3051141C (en) | 2017-01-23 | 2021-06-22 | Stemcell Technologies Canada Inc. | Media and methods for enhancing the survival and proliferation of stem cells |
-
2018
- 2018-01-23 CA CA3051141A patent/CA3051141C/en active Active
- 2018-01-23 EP EP24162728.0A patent/EP4361256A3/en active Pending
- 2018-01-23 EP EP18741389.3A patent/EP3571290B1/en active Active
- 2018-01-23 SG SG11201906731XA patent/SG11201906731XA/en unknown
- 2018-01-23 WO PCT/CA2018/050076 patent/WO2018132926A1/en not_active Ceased
- 2018-01-23 AU AU2018209127A patent/AU2018209127B2/en active Active
- 2018-01-23 CN CN201880019192.7A patent/CN110462025B/zh active Active
- 2018-01-23 US US16/480,025 patent/US11697796B2/en active Active
- 2018-01-23 JP JP2019539835A patent/JP7049347B2/ja active Active
-
2019
- 2019-07-21 IL IL268194A patent/IL268194B/en unknown
-
2023
- 2023-03-15 US US18/184,517 patent/US20230250392A1/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101052712A (zh) * | 2004-09-08 | 2007-10-10 | 威斯康星校友研究基金会 | 培养人胚胎干细胞 |
| CN101182464A (zh) * | 2006-05-16 | 2008-05-21 | 贝克顿·迪金森公司 | 用于细胞培养的经细胞外基质包被的表面 |
| JP2009542247A (ja) * | 2006-07-12 | 2009-12-03 | ユニヴァーシティー オヴ シェフィールド | 細胞増殖培地 |
| JP2012175962A (ja) * | 2011-01-31 | 2012-09-13 | National Institute Of Biomedical Innovation | ヒト多能性幹細胞の培養方法 |
| CN105283541A (zh) * | 2013-05-30 | 2016-01-27 | 味之素株式会社 | 干细胞培养用培养基 |
| CA2981277A1 (en) * | 2015-03-30 | 2016-10-06 | Ajinomoto Co., Inc. | Human serum albumin-containing culture medium for growth of neural stem cells |
Non-Patent Citations (1)
| Title |
|---|
| Effect of ROCK inhibitor Y-27632 on normal and variant human embryonic stem cells (hESCs) in vitro: its benefits in hESC expansion;Kalamegam Gauthaman et al.;《Stem Cell Rev Rep》;20100331;第6卷(第1期);摘要 * |
Also Published As
| Publication number | Publication date |
|---|---|
| US20230250392A1 (en) | 2023-08-10 |
| US20200017825A1 (en) | 2020-01-16 |
| SG11201906731XA (en) | 2019-08-27 |
| EP4361256A2 (en) | 2024-05-01 |
| IL268194A (en) | 2019-09-26 |
| AU2018209127A1 (en) | 2019-08-08 |
| EP4361256A3 (en) | 2024-07-31 |
| CA3051141A1 (en) | 2018-07-26 |
| EP3571290A4 (en) | 2020-10-28 |
| JP2020505038A (ja) | 2020-02-20 |
| IL268194B (en) | 2022-05-01 |
| CN110462025A (zh) | 2019-11-15 |
| EP3571290B1 (en) | 2024-04-10 |
| US11697796B2 (en) | 2023-07-11 |
| EP3571290A1 (en) | 2019-11-27 |
| WO2018132926A1 (en) | 2018-07-26 |
| AU2018209127B2 (en) | 2021-10-21 |
| JP7049347B2 (ja) | 2022-04-06 |
| CA3051141C (en) | 2021-06-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110462025B (zh) | 增强干细胞存活和增殖的培养基和方法 | |
| CN103589683B (zh) | 一种脐带间充质干细胞的分离方法和培养方法 | |
| CN1871343B (zh) | 从脂肪组织制备干细胞的方法和系统 | |
| US9339520B2 (en) | Methods and compositions for treatment of bone defects with placental cell populations | |
| KR102862875B1 (ko) | 배양 배지 | |
| EP3074505B1 (en) | Serum-free medium | |
| JP2008271971A (ja) | 脂肪由来前駆細胞の細胞分化 | |
| CN112608891A (zh) | 一种间充质干细胞无血清培养基及其应用 | |
| WO2021254296A1 (zh) | 一种生物活性物质组合物、包含所述组合物的无血清培养基及其用途 | |
| Sardesai et al. | Avoidance of maternal cell contamination and overgrowth in isolating fetal chorionic villi mesenchymal stem cells from human term placenta | |
| CN113692282A (zh) | 一种生物活性物质组合物、包含所述组合物的无血清培养基及其用途 | |
| EP3963050B1 (en) | Preparation of human allogeneic liver-derived progenitor cells | |
| Araujo et al. | Long-term in vitro culture of bovine preantral follicles: effect of base medium and medium replacement methods | |
| US20220220445A1 (en) | Preparation of human allogeneic liver-derived progenitor cells | |
| HK40110808A (en) | Media and methods for enhancing the survival and proliferation of stem cells | |
| HK40017314A (en) | Media and methods for enhancing the survival and proliferation of stem cells | |
| HK40017314B (en) | Media and methods for enhancing the survival and proliferation of stem cells | |
| JP2024529729A (ja) | 加齢関連疾患および神経変性疾患の予防および処置におけるiPSC由来免疫細胞 | |
| Korovina | The use of bovine multipotent mesenchymal stem cells isolated from bone marrow and adipose tissue as sources to obtain muscle cells in vitro | |
| JP7758411B1 (ja) | 間葉系幹細胞培養物の製造方法 | |
| Dehdilani et al. | Enhanced Cultivation of Chicken Primordial Germ Cells: A Comparison of Enriched and Defined Media | |
| WO2025253985A1 (ja) | 間葉系幹細胞培養物の製造方法 | |
| TWI403582B (zh) | 促使神經幹細胞增生及/或分化的培養基 | |
| HK40068803B (en) | Preparation of human allogeneic liver-derived progenitor cells | |
| HK40068803A (en) | Preparation of human allogeneic liver-derived progenitor cells |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TG01 | Patent term adjustment | ||
| TG01 | Patent term adjustment |