CA3051141C - Media and methods for enhancing the survival and proliferation of stem cells - Google Patents

Media and methods for enhancing the survival and proliferation of stem cells Download PDF

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Publication number
CA3051141C
CA3051141C CA3051141A CA3051141A CA3051141C CA 3051141 C CA3051141 C CA 3051141C CA 3051141 A CA3051141 A CA 3051141A CA 3051141 A CA3051141 A CA 3051141A CA 3051141 C CA3051141 C CA 3051141C
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Canada
Prior art keywords
acid
stem cells
lipid
culture medium
cells
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CA3051141A
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English (en)
French (fr)
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CA3051141A1 (en
Inventor
Adam HIRST
Melanie KARDEL
Wing CHANG
Michael Hiatt
Eric Jervis
Arwen HUNTER
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Stemcell Technologies Canada Inc
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Stemcell Technologies Canada Inc
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Application filed by Stemcell Technologies Canada Inc filed Critical Stemcell Technologies Canada Inc
Publication of CA3051141A1 publication Critical patent/CA3051141A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA3051141A 2017-01-23 2018-01-23 Media and methods for enhancing the survival and proliferation of stem cells Active CA3051141C (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201762449413P 2017-01-23 2017-01-23
US62/449,413 2017-01-23
US201762518776P 2017-06-13 2017-06-13
US62/518,776 2017-06-13
US201762608875P 2017-12-21 2017-12-21
US62/608,875 2017-12-21
PCT/CA2018/050076 WO2018132926A1 (en) 2017-01-23 2018-01-23 Media and methods for enhancing the survival and proliferation of stem cells

Publications (2)

Publication Number Publication Date
CA3051141A1 CA3051141A1 (en) 2018-07-26
CA3051141C true CA3051141C (en) 2021-06-22

Family

ID=62907538

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3051141A Active CA3051141C (en) 2017-01-23 2018-01-23 Media and methods for enhancing the survival and proliferation of stem cells

Country Status (9)

Country Link
US (2) US11697796B2 (enExample)
EP (2) EP3571290B1 (enExample)
JP (1) JP7049347B2 (enExample)
CN (1) CN110462025B (enExample)
AU (1) AU2018209127B2 (enExample)
CA (1) CA3051141C (enExample)
IL (1) IL268194B (enExample)
SG (1) SG11201906731XA (enExample)
WO (1) WO2018132926A1 (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3571290B1 (en) 2017-01-23 2024-04-10 Stemcell Technologies Canada Inc. Media and methods for enhancing the survival and proliferation of stem cells
CN111808797A (zh) * 2020-07-28 2020-10-23 湖南赛诺生物科技股份有限公司 Necrostatin-1的应用及促进新生猪胰岛细胞成熟的制剂
JP2022032793A (ja) * 2020-08-14 2022-02-25 アルブキュラ コーポレーション アルブミン組成物並びにそれを製造及び使用する方法
JP2023539775A (ja) 2020-09-02 2023-09-19 ワイゼットセラピューティク パフォーマンス リミテッド 疾患、障害又は状態の処置のための細胞組成物及び使用方法
WO2022114724A1 (ko) * 2020-11-27 2022-06-02 (주)엑셀세라퓨틱스 고효율 및 고순도 엑소좀 생산용 배지 조성물
EP4293106A4 (en) * 2021-02-15 2024-07-31 Toppan Inc. METHOD FOR PRODUCING A THREE-DIMENSIONAL TISSUE BODY AND METHOD FOR PROMOTING THE DIFFERENTIATION OF STEM CELLS DERIVED FROM ADIPOSE TISSUE
CN116121186A (zh) * 2023-01-03 2023-05-16 内蒙古奥普赛生物科技有限公司 一种利用棕榈油酸培养脂肪间充质干细胞的方法
JP2026513626A (ja) * 2023-04-20 2026-04-28 ステムセル テクノロジーズ カナダ インコーポレイテッド Ｐｓｃの大規模増殖のための方法
CN116814532B (zh) * 2023-07-10 2025-02-11 上海迈邦生物科技有限公司 一种用于哺乳动物细胞培养基的脂类复合物及其制备方法与应用

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2001508302A (ja) 1997-01-10 2001-06-26 ライフ テクノロジーズ，インコーポレイテッド 胚性幹細胞血清置換
BRPI0514778B1 (pt) 2004-09-08 2018-07-10 Wisconsin Alumni Research Foundation Meio para cultivar células tronco embrionárias
KR101107835B1 (ko) * 2006-01-13 2012-02-09 가부시키가이샤 투셀 동물 세포의 무혈청 배양에 사용하기 위한 배양 배지 첨가물, 키트 및 이의 용도
US8241908B2 (en) 2006-05-16 2012-08-14 Becton, Dickinson And Company Extracellular matrix coated surface for culturing cells
GB0613756D0 (en) 2006-07-12 2006-08-23 Univ Sheffield Cell culture medium
ES2360782B1 (es) 2009-07-28 2012-03-12 Grifols, S.A. Medios para cultivo de células de mamíferos que comprenden sobrenadante de etapas del fraccionamiento de Cohn y uso de los mismos.
FI20096288A0 (fi) 2009-12-04 2009-12-04 Kristiina Rajala Formulations and methods for culturing stem cells
US10323919B2 (en) 2010-01-06 2019-06-18 Ervin Industries, Inc. Frangible, ceramic-metal composite objects and methods of making the same
JP6148429B2 (ja) 2011-01-31 2017-06-14 協和発酵バイオ株式会社 ヒト多能性幹細胞の培養方法
US8497124B2 (en) 2011-12-05 2013-07-30 Factor Bioscience Inc. Methods and products for reprogramming cells to a less differentiated state
MX354775B (es) 2012-03-07 2018-03-20 Janssen Biotech Inc Medios definidos para la expansion y el mantenimiento de celulas madre pluripotentes.
CN105531365A (zh) * 2013-04-23 2016-04-27 耶达研究及发展有限公司 分离的幼稚多能干细胞和产生所述细胞的方法
SG11201509767UA (en) 2013-05-30 2015-12-30 Ajinomoto Kk Medium for culturing stem cells
WO2015042356A1 (en) 2013-09-19 2015-03-26 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Chemically defined culture medium for stem cell maintenance and differentiation
EP3279318A4 (en) * 2015-03-30 2018-08-29 Ajinomoto Co., Inc. Human serum albumin-containing culture medium for growth of neural stem cells
EP3571290B1 (en) 2017-01-23 2024-04-10 Stemcell Technologies Canada Inc. Media and methods for enhancing the survival and proliferation of stem cells

Also Published As

Publication number Publication date
EP3571290B1 (en) 2024-04-10
US11697796B2 (en) 2023-07-11
EP3571290A4 (en) 2020-10-28
CA3051141A1 (en) 2018-07-26
IL268194A (en) 2019-09-26
WO2018132926A1 (en) 2018-07-26
EP4361256A3 (en) 2024-07-31
AU2018209127B2 (en) 2021-10-21
JP2020505038A (ja) 2020-02-20
US20200017825A1 (en) 2020-01-16
CN110462025A (zh) 2019-11-15
EP3571290A1 (en) 2019-11-27
IL268194B (en) 2022-05-01
CN110462025B (zh) 2023-11-21
AU2018209127A1 (en) 2019-08-08
US20230250392A1 (en) 2023-08-10
JP7049347B2 (ja) 2022-04-06
EP4361256A2 (en) 2024-05-01
SG11201906731XA (en) 2019-08-27

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