CA3051141C - Media and methods for enhancing the survival and proliferation of stem cells - Google Patents

Media and methods for enhancing the survival and proliferation of stem cells Download PDF

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Publication number
CA3051141C
CA3051141C CA3051141A CA3051141A CA3051141C CA 3051141 C CA3051141 C CA 3051141C CA 3051141 A CA3051141 A CA 3051141A CA 3051141 A CA3051141 A CA 3051141A CA 3051141 C CA3051141 C CA 3051141C
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Prior art keywords
acid
stem cells
lipid
culture medium
cells
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CA3051141A
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English (en)
French (fr)
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CA3051141A1 (en
Inventor
Adam HIRST
Melanie KARDEL
Wing CHANG
Michael Hiatt
Eric Jervis
Arwen HUNTER
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Stemcell Technologies Canada Inc
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Stemcell Technologies Canada Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA3051141A 2017-01-23 2018-01-23 Media and methods for enhancing the survival and proliferation of stem cells Active CA3051141C (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201762449413P 2017-01-23 2017-01-23
US62/449,413 2017-01-23
US201762518776P 2017-06-13 2017-06-13
US62/518,776 2017-06-13
US201762608875P 2017-12-21 2017-12-21
US62/608,875 2017-12-21
PCT/CA2018/050076 WO2018132926A1 (en) 2017-01-23 2018-01-23 Media and methods for enhancing the survival and proliferation of stem cells

Publications (2)

Publication Number Publication Date
CA3051141A1 CA3051141A1 (en) 2018-07-26
CA3051141C true CA3051141C (en) 2021-06-22

Family

ID=62907538

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3051141A Active CA3051141C (en) 2017-01-23 2018-01-23 Media and methods for enhancing the survival and proliferation of stem cells

Country Status (9)

Country Link
US (2) US11697796B2 (enExample)
EP (2) EP4361256A3 (enExample)
JP (1) JP7049347B2 (enExample)
CN (1) CN110462025B (enExample)
AU (1) AU2018209127B2 (enExample)
CA (1) CA3051141C (enExample)
IL (1) IL268194B (enExample)
SG (1) SG11201906731XA (enExample)
WO (1) WO2018132926A1 (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3051141C (en) 2017-01-23 2021-06-22 Stemcell Technologies Canada Inc. Media and methods for enhancing the survival and proliferation of stem cells
CN111808797A (zh) * 2020-07-28 2020-10-23 湖南赛诺生物科技股份有限公司 Necrostatin-1的应用及促进新生猪胰岛细胞成熟的制剂
JP2022032793A (ja) * 2020-08-14 2022-02-25 アルブキュラ コーポレーション アルブミン組成物並びにそれを製造及び使用する方法
IL300809A (en) 2020-09-02 2023-04-01 Yztherapeutic Performance Ltd Cellular preparation for the treatment of diseases and method of use
WO2022114724A1 (ko) * 2020-11-27 2022-06-02 (주)엑셀세라퓨틱스 고효율 및 고순도 엑소좀 생산용 배지 조성물
WO2022173058A1 (ja) * 2021-02-15 2022-08-18 凸版印刷株式会社 三次元組織体の製造方法及び脂肪由来幹細胞の分化促進方法
CN116121186A (zh) * 2023-01-03 2023-05-16 内蒙古奥普赛生物科技有限公司 一种利用棕榈油酸培养脂肪间充质干细胞的方法
WO2024216399A1 (en) * 2023-04-20 2024-10-24 Stemcell Technologies Canada Inc. Methods for large scale expansion of pluripotent stem cells (pscs)
CN116814532B (zh) * 2023-07-10 2025-02-11 上海迈邦生物科技有限公司 一种用于哺乳动物细胞培养基的脂类复合物及其制备方法与应用

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0986635A4 (en) 1997-01-10 2001-11-07 Life Technologies Inc SERUM SUBSTITUTE FOR EMBRYONIC STEM CELLS
ES2383813T3 (es) 2004-09-08 2012-06-26 Wisconsin Alumni Research Foundation Método de cultivo y cultivo de células madre embrionarias
US9074176B2 (en) * 2006-01-13 2015-07-07 Two Cells Co., Ltd. Culture medium additive for use in serum-free culturing of animal cell, kit and use thereof
US8241908B2 (en) 2006-05-16 2012-08-14 Becton, Dickinson And Company Extracellular matrix coated surface for culturing cells
GB0613756D0 (en) 2006-07-12 2006-08-23 Univ Sheffield Cell culture medium
ES2360782B1 (es) 2009-07-28 2012-03-12 Grifols, S.A. Medios para cultivo de células de mamíferos que comprenden sobrenadante de etapas del fraccionamiento de Cohn y uso de los mismos.
FI20096288A0 (fi) * 2009-12-04 2009-12-04 Kristiina Rajala Formulations and methods for culturing stem cells
WO2011085072A2 (en) 2010-01-06 2011-07-14 Ervin Industries, Inc. Frangible, ceramic-metal composite objects and methods of making the same
JP6148429B2 (ja) 2011-01-31 2017-06-14 協和発酵バイオ株式会社 ヒト多能性幹細胞の培養方法
US8497124B2 (en) * 2011-12-05 2013-07-30 Factor Bioscience Inc. Methods and products for reprogramming cells to a less differentiated state
RU2018128383A (ru) 2012-03-07 2019-03-14 Янссен Байотек, Инк. Среда определенного состава для размножения и обновления плюрипотентных стволовых клеток
JP2016521971A (ja) * 2013-04-23 2016-07-28 イェダ リサーチ アンド ディベロップメント カンパニー リミテッド 単離ナイーブ型多能性幹細胞およびそれを発生させる方法関連出願本出願は、米国特許法１１９条第（ｅ）項に基づき、２０１４年１月２９日出願の米国特許仮出願第６１／９３２，９３５号、２０１３年９月１７日出願の米国特許仮出願第６１／８７８，７６９号、および２０１３年４月２３日出願の米国特許仮出願第６１／８１４，９２０号の優先権を主張する。また、本出願は、同時に提出された同出願人による同時係属出願である、ＹａｑｕｂＨＡＮＮＡ、ＮｏａＮＯＶＥＲＳＨＴＥＲＮ、およびＹｏａｃｈＲＡＩＳによる米国特許出願（発明の名称「単離ナイーブ型多能性幹細胞およびそれを発生させる方法（ＩＳＯＬＡＴＥＤＮＡＩＶＥＰＬＵＲＩＰＯＴＥＮＴＳＴＥＭＣＥＬＬＳＡＮＤＭＥＴＨＯＤＳＯＦＧＥＮＥＲＡＴＩＮＧＳＡＭＥ）」）（代理人事件記録簿第５８８７０号）にも関する。上記出願の内容はその全体を参考として本明細書に組み込む。
SG11201509767UA (en) 2013-05-30 2015-12-30 Ajinomoto Kk Medium for culturing stem cells
WO2015042356A1 (en) * 2013-09-19 2015-03-26 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Chemically defined culture medium for stem cell maintenance and differentiation
CA2981277A1 (en) * 2015-03-30 2016-10-06 Ajinomoto Co., Inc. Human serum albumin-containing culture medium for growth of neural stem cells
CA3051141C (en) 2017-01-23 2021-06-22 Stemcell Technologies Canada Inc. Media and methods for enhancing the survival and proliferation of stem cells

Also Published As

Publication number Publication date
US20230250392A1 (en) 2023-08-10
US20200017825A1 (en) 2020-01-16
SG11201906731XA (en) 2019-08-27
EP4361256A2 (en) 2024-05-01
IL268194A (en) 2019-09-26
AU2018209127A1 (en) 2019-08-08
EP4361256A3 (en) 2024-07-31
CA3051141A1 (en) 2018-07-26
EP3571290A4 (en) 2020-10-28
JP2020505038A (ja) 2020-02-20
IL268194B (en) 2022-05-01
CN110462025A (zh) 2019-11-15
EP3571290B1 (en) 2024-04-10
US11697796B2 (en) 2023-07-11
CN110462025B (zh) 2023-11-21
EP3571290A1 (en) 2019-11-27
WO2018132926A1 (en) 2018-07-26
AU2018209127B2 (en) 2021-10-21
JP7049347B2 (ja) 2022-04-06

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