JP7049347B2 - 幹細胞の生存および増殖を高めるための培地および方法 - Google Patents

幹細胞の生存および増殖を高めるための培地および方法 Download PDF

Info

Publication number
JP7049347B2
JP7049347B2 JP2019539835A JP2019539835A JP7049347B2 JP 7049347 B2 JP7049347 B2 JP 7049347B2 JP 2019539835 A JP2019539835 A JP 2019539835A JP 2019539835 A JP2019539835 A JP 2019539835A JP 7049347 B2 JP7049347 B2 JP 7049347B2
Authority
JP
Japan
Prior art keywords
acid
stem cells
culture medium
lipid
cells
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2019539835A
Other languages
English (en)
Japanese (ja)
Other versions
JP2020505038A (ja
JP2020505038A5 (enExample
Inventor
アダム ハースト
メラニー カーデル
ウィン チャン
マイケル ハイアット
エリック ジャービス
アーウェン ハンター
Original Assignee
ステムセル テクノロジーズ カナダ インコーポレイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=62907538&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=JP7049347(B2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by ステムセル テクノロジーズ カナダ インコーポレイテッド filed Critical ステムセル テクノロジーズ カナダ インコーポレイテッド
Publication of JP2020505038A publication Critical patent/JP2020505038A/ja
Publication of JP2020505038A5 publication Critical patent/JP2020505038A5/ja
Application granted granted Critical
Publication of JP7049347B2 publication Critical patent/JP7049347B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/36—Lipids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2019539835A 2017-01-23 2018-01-23 幹細胞の生存および増殖を高めるための培地および方法 Active JP7049347B2 (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201762449413P 2017-01-23 2017-01-23
US62/449,413 2017-01-23
US201762518776P 2017-06-13 2017-06-13
US62/518,776 2017-06-13
US201762608875P 2017-12-21 2017-12-21
US62/608,875 2017-12-21
PCT/CA2018/050076 WO2018132926A1 (en) 2017-01-23 2018-01-23 Media and methods for enhancing the survival and proliferation of stem cells

Publications (3)

Publication Number Publication Date
JP2020505038A JP2020505038A (ja) 2020-02-20
JP2020505038A5 JP2020505038A5 (enExample) 2021-07-26
JP7049347B2 true JP7049347B2 (ja) 2022-04-06

Family

ID=62907538

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2019539835A Active JP7049347B2 (ja) 2017-01-23 2018-01-23 幹細胞の生存および増殖を高めるための培地および方法

Country Status (9)

Country Link
US (2) US11697796B2 (enExample)
EP (2) EP4361256A3 (enExample)
JP (1) JP7049347B2 (enExample)
CN (1) CN110462025B (enExample)
AU (1) AU2018209127B2 (enExample)
CA (1) CA3051141C (enExample)
IL (1) IL268194B (enExample)
SG (1) SG11201906731XA (enExample)
WO (1) WO2018132926A1 (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3051141C (en) 2017-01-23 2021-06-22 Stemcell Technologies Canada Inc. Media and methods for enhancing the survival and proliferation of stem cells
CN111808797A (zh) * 2020-07-28 2020-10-23 湖南赛诺生物科技股份有限公司 Necrostatin-1的应用及促进新生猪胰岛细胞成熟的制剂
JP2022032793A (ja) * 2020-08-14 2022-02-25 アルブキュラ コーポレーション アルブミン組成物並びにそれを製造及び使用する方法
IL300809A (en) 2020-09-02 2023-04-01 Yztherapeutic Performance Ltd Cellular preparation for the treatment of diseases and method of use
WO2022114724A1 (ko) * 2020-11-27 2022-06-02 (주)엑셀세라퓨틱스 고효율 및 고순도 엑소좀 생산용 배지 조성물
WO2022173058A1 (ja) * 2021-02-15 2022-08-18 凸版印刷株式会社 三次元組織体の製造方法及び脂肪由来幹細胞の分化促進方法
CN116121186A (zh) * 2023-01-03 2023-05-16 内蒙古奥普赛生物科技有限公司 一种利用棕榈油酸培养脂肪间充质干细胞的方法
WO2024216399A1 (en) * 2023-04-20 2024-10-24 Stemcell Technologies Canada Inc. Methods for large scale expansion of pluripotent stem cells (pscs)
CN116814532B (zh) * 2023-07-10 2025-02-11 上海迈邦生物科技有限公司 一种用于哺乳动物细胞培养基的脂类复合物及其制备方法与应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009542247A (ja) 2006-07-12 2009-12-03 ユニヴァーシティー オヴ シェフィールド 細胞増殖培地
JP2012175962A (ja) 2011-01-31 2012-09-13 National Institute Of Biomedical Innovation ヒト多能性幹細胞の培養方法

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0986635A4 (en) 1997-01-10 2001-11-07 Life Technologies Inc SERUM SUBSTITUTE FOR EMBRYONIC STEM CELLS
ES2383813T3 (es) 2004-09-08 2012-06-26 Wisconsin Alumni Research Foundation Método de cultivo y cultivo de células madre embrionarias
US9074176B2 (en) * 2006-01-13 2015-07-07 Two Cells Co., Ltd. Culture medium additive for use in serum-free culturing of animal cell, kit and use thereof
US8241908B2 (en) 2006-05-16 2012-08-14 Becton, Dickinson And Company Extracellular matrix coated surface for culturing cells
ES2360782B1 (es) 2009-07-28 2012-03-12 Grifols, S.A. Medios para cultivo de células de mamíferos que comprenden sobrenadante de etapas del fraccionamiento de Cohn y uso de los mismos.
FI20096288A0 (fi) * 2009-12-04 2009-12-04 Kristiina Rajala Formulations and methods for culturing stem cells
WO2011085072A2 (en) 2010-01-06 2011-07-14 Ervin Industries, Inc. Frangible, ceramic-metal composite objects and methods of making the same
US8497124B2 (en) * 2011-12-05 2013-07-30 Factor Bioscience Inc. Methods and products for reprogramming cells to a less differentiated state
RU2018128383A (ru) 2012-03-07 2019-03-14 Янссен Байотек, Инк. Среда определенного состава для размножения и обновления плюрипотентных стволовых клеток
JP2016521971A (ja) * 2013-04-23 2016-07-28 イェダ リサーチ アンド ディベロップメント カンパニー リミテッド 単離ナイーブ型多能性幹細胞およびそれを発生させる方法関連出願本出願は、米国特許法１１９条第（ｅ）項に基づき、２０１４年１月２９日出願の米国特許仮出願第６１／９３２，９３５号、２０１３年９月１７日出願の米国特許仮出願第６１／８７８，７６９号、および２０１３年４月２３日出願の米国特許仮出願第６１／８１４，９２０号の優先権を主張する。また、本出願は、同時に提出された同出願人による同時係属出願である、ＹａｑｕｂＨＡＮＮＡ、ＮｏａＮＯＶＥＲＳＨＴＥＲＮ、およびＹｏａｃｈＲＡＩＳによる米国特許出願（発明の名称「単離ナイーブ型多能性幹細胞およびそれを発生させる方法（ＩＳＯＬＡＴＥＤＮＡＩＶＥＰＬＵＲＩＰＯＴＥＮＴＳＴＥＭＣＥＬＬＳＡＮＤＭＥＴＨＯＤＳＯＦＧＥＮＥＲＡＴＩＮＧＳＡＭＥ）」）（代理人事件記録簿第５８８７０号）にも関する。上記出願の内容はその全体を参考として本明細書に組み込む。
SG11201509767UA (en) 2013-05-30 2015-12-30 Ajinomoto Kk Medium for culturing stem cells
WO2015042356A1 (en) * 2013-09-19 2015-03-26 The United States Of America, As Represented By The Secretary, Department Of Health & Human Services Chemically defined culture medium for stem cell maintenance and differentiation
CA2981277A1 (en) * 2015-03-30 2016-10-06 Ajinomoto Co., Inc. Human serum albumin-containing culture medium for growth of neural stem cells
CA3051141C (en) 2017-01-23 2021-06-22 Stemcell Technologies Canada Inc. Media and methods for enhancing the survival and proliferation of stem cells

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009542247A (ja) 2006-07-12 2009-12-03 ユニヴァーシティー オヴ シェフィールド 細胞増殖培地
JP2012175962A (ja) 2011-01-31 2012-09-13 National Institute Of Biomedical Innovation ヒト多能性幹細胞の培養方法

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Biotechnol Prog.，2016年07月08日，Vol.32, No.4，pp.1009-1016
PLoS One，Vol.3, No.1, e1384，2008年，pp.1-10
Proc. Natl. Acad. Sci. USA.，2008年09月09日，Vol.105, No.36，pp.13409-13414
Stem Cell Rev. Rep.，2010年03月，Vol.6, No.1，pp.86-95

Also Published As

Publication number Publication date
US20230250392A1 (en) 2023-08-10
US20200017825A1 (en) 2020-01-16
SG11201906731XA (en) 2019-08-27
EP4361256A2 (en) 2024-05-01
IL268194A (en) 2019-09-26
AU2018209127A1 (en) 2019-08-08
EP4361256A3 (en) 2024-07-31
CA3051141A1 (en) 2018-07-26
EP3571290A4 (en) 2020-10-28
JP2020505038A (ja) 2020-02-20
IL268194B (en) 2022-05-01
CN110462025A (zh) 2019-11-15
EP3571290B1 (en) 2024-04-10
US11697796B2 (en) 2023-07-11
CN110462025B (zh) 2023-11-21
EP3571290A1 (en) 2019-11-27
WO2018132926A1 (en) 2018-07-26
AU2018209127B2 (en) 2021-10-21
CA3051141C (en) 2021-06-22

Similar Documents

Publication Publication Date Title
JP7049347B2 (ja) 幹細胞の生存および増殖を高めるための培地および方法
CN102791276B (zh) 含有间充质干细胞的细胞制品及其制造方法
EP1988159B1 (en) Additive for culture medium for use in serum-free culture of animal cell, kit, and use of the additive or kit
EP3031909B1 (en) Immunomodulation using placental stem cells
US9339520B2 (en) Methods and compositions for treatment of bone defects with placental cell populations
Sardesai et al. Avoidance of maternal cell contamination and overgrowth in isolating fetal chorionic villi mesenchymal stem cells from human term placenta
JP6634822B2 (ja) 幹細胞の培養用培地
CN106062179A (zh) 无血清培养基
EP3963050B1 (en) Preparation of human allogeneic liver-derived progenitor cells
US20220220445A1 (en) Preparation of human allogeneic liver-derived progenitor cells
WO2020200272A1 (zh) 人心肌细胞分离试剂、培养基、分离方法和培养方法
HK40110808A (en) Media and methods for enhancing the survival and proliferation of stem cells
HK40017314A (en) Media and methods for enhancing the survival and proliferation of stem cells
HK40017314B (en) Media and methods for enhancing the survival and proliferation of stem cells
CN101326280A (zh) 使用胎盘干细胞的免疫调节
KR20250084967A (ko) 신규 세포주 및 요법에서 이의 용도
TWI403582B (zh) 促使神經幹細胞增生及／或分化的培養基
HK40068803B (en) Preparation of human allogeneic liver-derived progenitor cells
HK40068803A (en) Preparation of human allogeneic liver-derived progenitor cells
HK1225753B (en) Immunomodulation using placental stem cells
HK1139707A (en) Methods and compositions for treatment of bone defects with placental cell populations
HK1178940A (en) Immunomodulation using placental stem cells

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20191010

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20190909

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20210118

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210525

A871 Explanation of circumstances concerning accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A871

Effective date: 20210525

RD04 Notification of resignation of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7424

Effective date: 20210615

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20210714

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20211012

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20211117

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20211221

C60 Trial request (containing other claim documents, opposition documents)

Free format text: JAPANESE INTERMEDIATE CODE: C60

Effective date: 20211221

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20220104

C21 Notice of transfer of a case for reconsideration by examiners before appeal proceedings

Free format text: JAPANESE INTERMEDIATE CODE: C21

Effective date: 20220105

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20220209

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20220209

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20220323

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20220325

R150 Certificate of patent or registration of utility model

Ref document number: 7049347

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250