CN110403832B - 一种负载有脂溶性维生素的桃胶多糖纳米球的制备方法 - Google Patents
一种负载有脂溶性维生素的桃胶多糖纳米球的制备方法 Download PDFInfo
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Abstract
本发明公开了一种负载有脂溶性维生素的桃胶多糖纳米球的制备方法。将1重量份的水溶性桃胶多糖、4~8重量份的硫辛酸和0.2~0.8重量份的对甲苯磺酸一同加入到50~100重量份的二甲基亚砜中，所得混合液在氮气氛围下于80~120℃搅拌反应5~12小时，然后用去离子水透析24小时后离心分离，收集离心得到的沉淀物；将收集的离心沉淀物和0.01~0.25重量份的脂溶性维生素一起加入到10~50重量份的有机溶剂中搅拌10分钟后，再在去离子水中透析24小时，即制得负载有脂溶性维生素的桃胶多糖纳米球。本发明具有制备过程简单、提纯分离方便、制备成本低等优点。所制备的负载有脂溶性维生素的桃胶多糖纳米球在功能性食品和化妆品领域具有良好的应用前景。
Description
技术领域
本发明属于功能性食品和化妆品领域，特别涉及一种负载有脂溶性维生素的桃胶多糖纳米球的制备方法。
背景技术
脂溶性维生素是指维生素A、维生素D、维生素E、维生素K等，这类型的维生素具有多种生物活性，是维持人体器官正常运行的必要营养成分。然而，该类型的维生素人体和动物体都难以自行合成，需通过外源性摄入才能达到维持正常活动的水平。由于脂溶性维生素存在容易氧化、对外部环境敏感、水溶性差等缺点，使其难以被人体和动物体有效吸收。目前，主要采用的解决手段为构筑一种纳米载体来负载脂溶性维生素。桃胶多糖是一种典型的天然高分子，具有生物相容性好和来源广泛的特点。本发明选用水溶性桃胶多糖和抗氧化剂硫辛酸为原料，通过简单的自组装交联来构筑纳米球。同时，该载体材料中的硫辛酸组分可以作为抗氧化剂，起到保护脂溶性维生素的作用。本发明所制备的负载有脂溶性维生素的桃胶多糖纳米球还未见有报道，无论从制备方法还是从原料方面，本发明都具有显著的创新性和强的实用性。
发明内容
本发明的目的在于提供一种负载有脂溶性维生素的桃胶多糖纳米球的制备方法，该制备方法的关键是先利用硫辛酸修饰桃胶多糖分子得到两亲性的桃胶多糖分子，再在水中与脂溶性维生素一起自组装交联来制得负载有脂溶性维生素的桃胶多糖纳米球。
具体步骤为：
(1) 将1重量份的水溶性桃胶多糖、4~8重量份的硫辛酸和0.2~0.8重量份的对甲苯磺酸一同加入到50~100重量份的二甲基亚砜中，所得混合液在氮气氛围下于80~120℃搅拌反应5~12小时，然后用去离子水透析24小时后离心分离，收集离心得到的沉淀物。
(2) 将步骤(1)收集的离心沉淀物和0.01~0.25重量份的脂溶性维生素一起加入到10~50重量份的有机溶剂中搅拌10分钟后，再在去离子水中透析24小时，即制得负载有脂溶性维生素的桃胶多糖纳米球。
所述的脂溶性维生素为维生素A、维生素D、维生素E和维生素K中的一种或多种混合物。
所述的有机溶剂为乙醇、四氢呋喃、异丙醇、二甲基亚砜和二甲基甲酰胺中的一种。
所述的化学试剂均为化学纯及以上纯度。
本发明具有制备过程简单、提纯分离方便、制备成本低等优点；制得的负载有脂溶性维生素的桃胶多糖纳米球具有优异的水分散性和稳定性，其水分散液可以静置三周而不出现明显沉淀物；用扫描电子显微镜和透射电子显微镜测试了其形貌和尺寸，结果证实了其具有球形结构，粒径大小为20~90纳米；该负载有脂溶性维生素的桃胶多糖纳米球在功能性食品和化妆品领域具有良好的应用前景。
附图说明
图1为本发明实施例1制备的负载有维生素A的桃胶多糖纳米球的水分散液照片。
图2为本发明实施例1制备的负载有维生素A的桃胶多糖纳米球的扫描电子显微镜照片。
图3为本发明实施例1制备的负载有维生素A的桃胶多糖纳米球的透射电子显微镜照片。
具体实施方式
实施例1:
(1) 将1克水溶性桃胶多糖、8克硫辛酸和0.8克对甲苯磺酸一同加入到60克二甲基亚砜中，所得混合液在氮气氛围下于95℃搅拌反应10小时，反应后的混合液用去离子水透析24小时后离心分离，收集离心得到的沉淀物。
(2) 将步骤(1)收集的离心沉淀物和0.25克维生素A一起加入到30克乙醇中搅拌10分钟后，再在去离子水中透析24小时，即制得负载有维生素A的桃胶多糖纳米球。
实施例2:
(1) 将1克水溶性桃胶多糖、4.5克硫辛酸和0.4克对甲苯磺酸一同加入到50克二甲基亚砜中，所得混合液在氮气氛围下于80℃搅拌反应12小时，反应后的混合液用去离子水透析24小时后离心分离，收集离心得到的沉淀物。
(2) 将步骤(1)收集的离心沉淀物和0.05克维生素D一起加入到10克乙醇中搅拌10分钟后，再在去离子水中透析24小时，即制得负载有维生素D的桃胶多糖纳米球。
实施例3:
(1) 将1.5克水溶性桃胶多糖、6克硫辛酸和0.6克对甲苯磺酸一同加入到75克二甲基亚砜中，所得混合液在氮气氛围下于120℃搅拌反应6小时，反应后的混合液用去离子水透析24小时后离心分离，收集离心得到的沉淀物。
(2) 将步骤(1)收集的离心沉淀物、0.1克维生素A和0.1克维生素D一起加入到30克四氢呋喃中搅拌10分钟后，再在去离子水中透析24小时，即制得同时负载有维生素A和维生素D的桃胶多糖纳米球。
实施例4:
(1) 将2克水溶性桃胶多糖、10克硫辛酸和1克对甲苯磺酸一同加入到200克二甲基亚砜中，所得混合液在氮气氛围下于90℃搅拌反应8小时，反应后的混合液用去离子水透析24小时后离心分离，收集离心得到的沉淀物。
(2) 将步骤(1)收集的离心沉淀物和0.05克维生素E一起加入到50克异丙醇中搅拌10分钟后，再在去离子水中透析24小时，即制得负载有维生素E的桃胶多糖纳米球。
实施例5:
(1) 将1克水溶性桃胶多糖、4克硫辛酸和0.2克对甲苯磺酸一同加入到50克二甲基亚砜中，所得混合液在氮气氛围下于110℃搅拌反应5小时，反应后的混合液用去离子水透析24小时后离心分离，收集离心得到的沉淀物。
(2) 将步骤(1)收集的离心沉淀物和0.01克维生素K一起加入到15克二甲基亚砜中搅拌10分钟后，再在去离子水中透析24小时，即制得负载有维生素K的桃胶多糖纳米球。
实施例6:
(1) 将1克水溶性桃胶多糖、6克硫辛酸和0.6克对甲苯磺酸一同加入到70克二甲基亚砜中，所得混合液在氮气氛围下于95℃搅拌反应10小时，反应后的混合液用去离子水透析24小时后离心分离，收集离心得到的沉淀物。
(2) 将步骤(1)收集的离心沉淀物、0.15克维生素E和0.1克维生素K一起加入到30克二甲基亚砜中搅拌10分钟后，再在去离子水中透析24小时，即制得同时负载有维生素E和维生素K的桃胶多糖纳米球。
实施例7:
(1) 将1.2克水溶性桃胶多糖、6克硫辛酸和0.6克对甲苯磺酸一同加入到75克二甲基亚砜中，所得混合液在氮气氛围下于100℃搅拌反应7小时，反应后的混合液用去离子水透析24小时后离心分离，收集离心得到的沉淀物。
(2) 将步骤(1)收集的离心沉淀物、0.05克维生素A、0.05克维生素D、0.05克维生素E和0.05克维生素K一起加入到60克二甲基甲酰胺中搅拌10分钟后，再在去离子水中透析24小时，即制得同时负载有维生素A、维生素D、维生素E和维生素K的桃胶多糖纳米球。
Claims (1)
1.一种负载有脂溶性维生素的桃胶多糖纳米球的制备方法，其特征在于具体步骤为：
(1) 将1重量份的水溶性桃胶多糖、4~8重量份的硫辛酸和0.2~0.8重量份的对甲苯磺酸一同加入到50~100重量份的二甲基亚砜中，所得混合液在氮气氛围下于80~120℃搅拌反应5~12小时，然后用去离子水透析24小时后离心分离，收集离心得到的沉淀物；
(2) 将步骤(1)收集的离心沉淀物和0.01~0.25重量份的脂溶性维生素一起加入到10~50重量份的有机溶剂中搅拌10分钟后，再在去离子水中透析24小时，即制得负载有脂溶性维生素的桃胶多糖纳米球；
所述的脂溶性维生素为维生素A、维生素D、维生素E和维生素K中的一种或多种混合物；
所述的有机溶剂为乙醇、四氢呋喃、异丙醇、二甲基亚砜和二甲基甲酰胺中的一种；
所述的化学试剂均为化学纯及以上纯度。
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