CN110199809A - A kind of efficient construction method of cassava inbred line population material - Google Patents

A kind of efficient construction method of cassava inbred line population material Download PDF

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CN110199809A
CN110199809A CN201910570560.3A CN201910570560A CN110199809A CN 110199809 A CN110199809 A CN 110199809A CN 201910570560 A CN201910570560 A CN 201910570560A CN 110199809 A CN110199809 A CN 110199809A
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cassava
fertilizer
application
inbred line
flower
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CN110199809B (en
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严华兵
陆柳英
谢向誉
曹升
尚小红
肖亮
王颖
曾文丹
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Guangxi Zhuang Nationality Autonomous Region Academy of Agricultural Sciences
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Guangxi Zhuang Nationality Autonomous Region Academy of Agricultural Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C21/00Methods of fertilising, sowing or planting
    • A01C21/005Following a specific plan, e.g. pattern
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/25Root crops, e.g. potatoes, yams, beet or wasabi
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B1/00Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
    • C05B1/02Superphosphates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B7/00Fertilisers based essentially on alkali or ammonium orthophosphates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00Other organic fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00Other organic fertilisers
    • C05F11/02Other organic fertilisers from peat, brown coal, and similar vegetable deposits
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80Soil conditioners
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Soil Sciences (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Botany (AREA)
  • Cultivation Of Plants (AREA)
  • Fertilizers (AREA)

Abstract

The invention discloses a kind of efficient construction methods of cassava inbred line population material, include the following steps: selection of land, level land and base manure application, sowing, rush Hua Baohua, pollination, fruit retention, harvesting seed.The present invention is using compound potassium sulfate fertilizer, calcium magnesium phosphate, organic fertilizer mixing fertilising; fertilizer each element ratio is suitable; meet the growth needs of Manihot Esculenta; it sprays or root application flower forcing is fertile, protects fertilizers for potted flowers, potassium dihydrogen phosphate; cassava is promoted to bloom ahead of time; raising is bloomed quantity and per plant rate, and the top dressing of fruit retention fertilizer is used after cassava result, effectively increases the setting percentage and fruit weight of cassava fruit.Interlacing interval of the present invention implantation time improves the collision probability of cassava hero female flower, improves pollination rate.The present invention has also been discharged the interference of foreign pollen, and purity is up to 100%, and the present invention use fertilising, promote flower, protect flower, fruit retention method, so that cassava is bloomed ahead of time, seed harvests ahead of time, and seeding ratio improves, and shortens breeding cycle.

Description

A kind of efficient construction method of cassava inbred line population material
Technical field
The present invention relates to technical field of crop cultivation, and in particular to a kind of efficient side of building of cassava inbred line population material Method.
Background technique
Cassava (Manihot esculenta Crantz) is Euphorbiaceae casava, drought-enduring to resist barren, plantation extensively It is one of three big tuber crops in more than 100 countries such as Africa, America and Asia, the third-largest cereal crops in hot-zone, entirely The sixth-largest cereal crops of ball are known as " king of starch ".Cassava whole body be all it is precious, it is widely used, it is edible, feeding and be processed into Various industrial products, such as starch, alcohol.
Cassava is the different flowering plant of monoecism, and crossbreeding is the important channel of breed breeding.The cassava of China's breeding is new Kind is mainly introduced by resource or open hybridization (only knowing female parent) breeding come, part kind is in Hainan or from foreign countries It collects breeding hybridized.Up to the present, south China 205 is selected altogether, south China 5, newly selects 048 etc. more than 30 cassava new product Kind.The influence of main climate factor carries out crossbreeding difficulty south subtropics monsoon region (such as: in Guangxi District), in Guangxi Cassava fruit is difficult normal mature under weather conditions, there are fruit development is abnormal or the ropy outstanding problem of seed, passes through The excellent Cassava Variety that crossbreeding obtains is seldom, torrid areas (such as: Hainan) although cassava fruit energy normal mature, It is the cassava florescence vulnerable to typhoon influence, cassava pollination rate is low, directly results in cassava seed acquisition amount and substantially reduces, and it is new to extend cassava The breeding for quality period.Therefore, urgent need innovation breeding method, is in Guangxi District and cassava breeding and system are carried out in Hainan Region Select index work proposes solution.
The building of inbred line population and excellent resources, which are screened, provides important way for the excellent Cassava Variety breeding of characteristic Diameter.National tropical agriculture research center (CIAT) expert has found precious " glutinous cassava " germ plasm resource in self-mating system.More It works importantly, selfing recurrent selection can create pure lines material for cassava genetic improvement.Climate influences, and adds cassava It is cross pollinated plant, same inflorescence female flower male flower flowering time has interval, and the pollination rate of breeding is extremely low, and fruit-setting rate is extremely low, from Friendship is that population material is difficult to efficiently obtain.Up to the present, the acquisition in relation to being selfed based material is all by artificial miscellaneous in the world The method of friendship obtains, at high cost, low efficiency, the domestic report that inbred line population material obtains not yet.
Summary of the invention
The present invention overcomes in the prior art, under the weather conditions of south subtropics monsoon region cassava fruit be difficult normally at Ripe, abnormal there are fruit development or seed is of poor quality, in torrid areas, cassava pollination rate is low, and cassava seed acquisition amount is few, and The same inflorescence female flower male flower flowering time of cassava has interval, and the pollination rate of breeding is extremely low, and fruit-setting rate is extremely low, and uses artificial hybridization The technical problems such as the at high cost, low efficiency of the method for obtained inbred line population material provide a kind of cassava inbred line population material Efficient construction method.
To solve the above problems, the present invention adopts the following technical scheme that:
A kind of efficient construction method of cassava inbred line population material, includes the following steps:
(1) in the torrid zone and south subtropics monsoon region, the sandy soil of the deep loosing soil of soil layer, level land or mountain selection of land: are selected Ground, planting site irrigation and drainage are convenient and 5 kilometer range of periphery is interior plants without other cassavas;
(2) level land and apply with base manure: according to the line-spacing ridging of 1.0~1.2m, setting spacing in the rows is 1.0~1.2m on ridge Kind plant hole;Compound potassium sulfate fertilizer, calcium magnesium phosphate, organic fertilizer are spread fertilizer over the fields in described kind of plant hole;
(3) sow: in mid or late March to early April, interlacing is staggered 5~10 days and plants, and the seedling stem of varieties of plant is laid flat Or oblique cutting is seeded in kind of plant hole;
(4) promote Hua Baohua: in early June to late July, using flower forcing fertilizer;The method of application of the flower forcing fertilizer can be spray Flower forcing Liquid Fertilizer or root application flower forcing solid fertilizer are applied, the flower forcing solid fertilizer amount of application is 30~80kg/hm2, interval is applied for 5~10 days It with primary, sprays 2~3 times, full blossom stops application;Pay attention to field management later, protect bud, deactivate interval 5 after flower forcing fertilizer~ 10 days can root application protect fertilizers for potted flowers or spray 0.075~0.125% potassium dihydrogen phosphate for 1~2 time;
(5) pollinate: can completely by strictly open spontaneous pollination, can also by artificial supplementary pollination, as a result after can bagging Protection prevents from being scattered after seed is mature;5~15 days root application fruit retention fertilizer after cassava pollination;The dose of the fruit retention fertilizer be 50~ 100kg/hm2
(6) harvest seed: can be harvested successively January in November to next year mature selfed seed and in 5 DEG C~10 DEG C of temperature, It is saved in 60% Freezing room of humidity.
Wherein, in the flower forcing Liquid Fertilizer, mineral humic acid >=50g/L, EDTA- iron >=0.5g/L, EDTA- copper >= 0.5g/L, potassium fulvate >=14g/L, potassium sulfate >=5g/L, magnesium sulfate >=5g/L, potassium dihydrogen phosphate >=10g/L, dipotassium hydrogen phosphate ≥5g/L。
Wherein, in the flower forcing solid fertilizer, N+P2O5+K2O gross mass percentage composition >=5%, middle microelement >=8%, have Machine matter mass percentage >=15%, pH value 5.5~6.5.Wherein, the middle microelement is by calcium, magnesium, boron, iron, selenium, zinc member Element composition.Preferably, calcium, magnesium, boron, iron, selenium, zinc mass ratio are 10~12:0.1~0.5:0.01~0.1:0.1~0.3: 0.01~0.1:0.2~0.5.
Wherein, guarantor's fertilizers for potted flowers is alga fertilizer, in the alga fertilizer, alginic acid mass percentage >=36%.
Wherein, guarantor's fertilizers for potted flowers amount of application is 50~100kg/hm2
Wherein, in the fruit retention fertilizer, humic acid >=40g/L, organic matter >=110g/L, N >=150g/L, P2O5≥160g/L、 K2O >=190g/L, B:15~25g/L.
Wherein, the amount of application of the compound potassium sulfate fertilizer is 100~300kg/hm2, the amount of application of the calcium magnesium phosphate is 200~400kg/hm2, the amount of application of the organic fertilizer is 600~1000kgm2
Wherein, in the compound potassium sulfate fertilizer, N mass percentage 15%, P2O5Mass percentage 15%, K2O mass Percentage composition 15%.
Wherein, in the calcium magnesium phosphate, P2O5Mass percentage >=12%, MgO mass percentage >=4%, CaO matter Measure percentage composition >=10%.
Wherein, the organic fertilizer is made of addition microbial bacteria fermentation after sheep dung, cow dung, paulownia bran, phosphate fertilizer mixing, described micro- Biological bacteria is made of cellulose-decomposing bacterium, starch decomposer, lichengermium, nitrogen-fixing bacteria, EM bacterium, bacillus thuringiensis, institute It states in organic fertilizer, quality of organic matter percentage composition >=45%, N P and K mass percentage >=5%.
In step (3) sowing, the interlacing is staggered 5~10 days implantation methods are as follows: counts since the side of cassava planting site Number, odd-numbered line cassava are planted on the same day, and even number line cassava is planted on the same day;Implantation time between odd-numbered line and even number line differs 5~ 10 days.
Wherein, the seed that the step (6) obtains is nursery at 25~35 DEG C in room temperature.
When the breeding material that the present invention is obtained carries out breeding plantation, it need to will store, after about 2 months dormant periods, Cassava seed can normally sow vernalization, generally carry out under 25~35 DEG C of environment of temperature indoors, seedling can be moved from friendship by April in large bamboo hat with a conical crown and broad brim year It is that group plants to crop field.
The present invention has the advantages that compared with prior art
(1) a kind of efficient construction method of cassava inbred line population material of the invention uses potassium sulfate compound before planting Fertilizer, calcium magnesium phosphate, organic fertilizer mixing fertilising, wherein nitrogen K-Mg-Ca phosphorus ratio is suitable for that ensure that the nutrient growth of Manihot Esculenta Needs, in turn avoid plant excessive growth influence reproductive growth;It sprays or root application flower forcing fertilizer and sprays and protect fertilizers for potted flowers or potassium dihydrogen phosphate, It can promote cassava to bloom ahead of time, improve the quantity of blooming of cassava, improve per plant rate.It is chased after after cassava result using fruit retention fertilizer Fertilizer effectively increases the setting percentage and fruit weight of cassava fruit, improves cassava fruit quality, and interlacing interval 5 of the present invention ~10 days plantation cassavas improve the same period collision probability of cassava hero female flower, pollination rate are improved, to improve per plant rate.This The interference of foreign pollen has been discharged in the cassava inbred line population material selective breeding method of invention, and purity is up to 100%, and the present invention adopts Fertilising promotees flower, protects flower, fruit retention method, so that cassava is bloomed ahead of time, seed harvests ahead of time, it is ensured that the sowing of second year seed It can be transplanted to crop field after nursery in time, normal growth, shorten breeding cycle 1 year or more.
(2) the efficient construction method of cassava inbred line population material of the invention passes through reasonably cultivation and reasonable rush Flower, guarantor's flower, the available seed quality of fruit retention technology are good, the inbred line population material more than quantity, can get a large amount of obviously characters point From progeny population material, can therefrom screen to obtain excellent Cassava Cultivars (being), be conducive to cassava breeding and cassava heredity changes What skilled worker made goes on smoothly.
Specific embodiment
Below with reference to embodiment and test, the invention will be further described.
Embodiment 1
A kind of efficient construction method of cassava inbred line population material, includes the following steps:
(1) in the torrid zone and south subtropics monsoon region, the sandy soil of the deep loosing soil of soil layer, level land or mountain selection of land: are selected Ground, planting site irrigation and drainage are convenient and 5 kilometer range of periphery is interior plants without other cassavas;
(2) it levels land and is applied with base manure: according to the line-spacing ridging of 1.2m, the kind plant hole that spacing in the rows is 1.0m is set on ridge; Compound potassium sulfate fertilizer, calcium magnesium phosphate, organic fertilizer are spread fertilizer over the fields in described kind of plant hole;The amount of application of the compound potassium sulfate fertilizer is 300kg/ hm2, the amount of application of the calcium magnesium phosphate is 200kg/hm2, the amount of application of the organic fertilizer is 1000kg/hm2;The potassium sulfate In compound fertilizer, N mass percentage 15%, P2O5Mass percentage 15%, K2O mass percentage 15%, the calcium and magnesium In phosphate fertilizer, P2O5Mass percentage 12%, MgO mass percentage 4%, CaO mass percentage 10%.The organic fertilizer It is made of addition microbial bacteria fermentation after the sheep dung of equal mass ratioes, cow dung, paulownia bran, phosphate fertilizer mixing, in the organic fertilizer, organic matter Mass percentage 45%, N P and K mass percentage 5%;The microbial bacteria by cellulose-decomposing bacterium, starch decomposer, Lichengermium, nitrogen-fixing bacteria, EM bacterium, bacillus thuringiensis composition;
(3) sow: in mid or late March to early April, interlacing is staggered 5 days and plants, and the seedling stem oblique cutting of varieties of plant is sowed In in kind of plant hole;It is to start counting from the side of cassava planting site that the interlacing, which is staggered 5 days and plants specific method, odd-numbered line cassava It plants on the same day, even number line cassava is planted on the same day;Implantation time between odd-numbered line and even number line differs 5 days;
(4) promote Hua Baohua: in early June to late July, using flower forcing fertilizer;The method of application of the flower forcing fertilizer is to spray Flower forcing Liquid Fertilizer is spaced application in 10 days once, sprays 3 times, and full blossom stops application;Field management is paid attention to later, is protected bud, is stopped It is protected fertilizers for potted flowers 2 times with 10 days root applications are spaced after flower forcing fertilizer;Guarantor's fertilizers for potted flowers amount of application is 100kg/hm2;Guarantor's fertilizers for potted flowers is seaweed Fertilizer;In the alga fertilizer, alginic acid mass percentage is 36%.In the flower forcing Liquid Fertilizer, mineral humic acid 50g/L, EDTA- iron 0.5g/L, EDTA- copper 0.5g/L, potassium fulvate 14g/L, potassium sulfate 5g/L, magnesium sulfate 5g/L, potassium dihydrogen phosphate 10g/L, dipotassium hydrogen phosphate 5g/L;
(5) pollinate: can completely by strictly open spontaneous pollination, can also by artificial supplementary pollination, as a result after can bagging Protection prevents from being scattered after seed is mature;15 days root application fruit retention fertilizer after cassava pollination;The dose of the fruit retention fertilizer is 50kg/ hm2;In the fruit retention fertilizer, humic acid 40g/L, organic matter 110g/L, N150g/L, P2O5160g/L、K2O190g/L、B25g/L。
(6) it harvests seed: mature selfed seed can be harvested successively January in November to next year and in 10 DEG C of temperature, humidity It is saved in 60% Freezing room;
(7) it nursery: when the inbred line population material that step (6) is obtained carries out breeding plantation, need to be stored about 2 months Dormant period after, carry out Pregermination and seedling breeding under 25 DEG C of environment of temperature indoors, seedling can be moved inbred line population and planted to big by April in large bamboo hat with a conical crown and broad brim year Field.
Embodiment 2
A kind of efficient construction method of cassava inbred line population material, includes the following steps:
(1) in the torrid zone and south subtropics monsoon region, the sandy soil of the deep loosing soil of soil layer, level land or mountain selection of land: are selected Ground, planting site irrigation and drainage are convenient and 5 kilometer range of periphery is interior plants without other cassavas;
(2) it levels land and is applied with base manure: according to the line-spacing ridging of 1.1m, the kind plant hole that spacing in the rows is 1.2m is set on ridge; Compound potassium sulfate fertilizer, calcium magnesium phosphate, organic fertilizer are spread fertilizer over the fields in described kind of plant hole;The amount of application of the compound potassium sulfate fertilizer is 200kg/ hm2, the amount of application of the calcium magnesium phosphate is 300kg/hm2, the amount of application of the organic fertilizer is 800kgm2;The potassium sulfate is compound In fertilizer, N mass percentage 15%, P2O5Mass percentage 15%, K2O mass percentage 15%, the calcium magnesium phosphate In, P2O5Mass percentage 20%, MgO mass percentage 5%, CaO mass percentage 12%;The organic fertilizer is by matter Amount for addition microbial bacteria fermentation after the sheep dung of 50:20:10:15, cow dung, paulownia bran, phosphate fertilizer mixing than being made, the organic fertilizer In, quality of organic matter percentage composition 55%, N P and K mass percentage 10%;The microbial bacteria by cellulose-decomposing bacterium, Starch decomposer, lichengermium, nitrogen-fixing bacteria, EM bacterium, bacillus thuringiensis composition;
(3) sow: in mid or late March to early April, interlacing is staggered 8 days and plants, and the seedling stem of varieties of plant is laid flat sowing In in kind of plant hole;It is to start counting from the side of cassava planting site that the interlacing, which is staggered 8 days and plants specific method, odd-numbered line cassava It plants on the same day, even number line cassava is planted on the same day;Implantation time between odd-numbered line and even number line differs 8 days;
(4) promote Hua Baohua: in early June to late July, using flower forcing fertilizer;The method of application of the flower forcing fertilizer is to spray Flower forcing Liquid Fertilizer is spaced application in 8 days once, sprays 2 times, and full blossom stops application;Field management is paid attention to later, is protected bud, is stopped It is sprayed within 8 days with being spaced after flower forcing fertilizer 0.075 potassium dihydrogen phosphate for 2 times;In the flower forcing Liquid Fertilizer, mineral humic acid 52g/L, EDTA- iron 0.8g/L, EDTA- copper 0.6g/L, potassium fulvate 20g/L, potassium sulfate 8g/L, magnesium sulfate 8g/L, potassium dihydrogen phosphate 12g/L, dipotassium hydrogen phosphate 8g/L;
(5) pollinate: can completely by strictly open spontaneous pollination, can also by artificial supplementary pollination, as a result after can bagging Protection prevents from being scattered after seed is mature;10 days root application fruit retention fertilizer after cassava pollination;The dose of the fruit retention fertilizer is 80kg/ hm2;In the fruit retention fertilizer, humic acid 50g/L, organic matter 114g/L, N160g/L, P2O5179g/L、K2O200g/L,B20g/L;
(6) it harvests seed: mature selfed seed can be harvested successively January in November to next year and in 8 DEG C of temperature, humidity It is saved in 60% Freezing room;
(7) it nursery: when the inbred line population material that step (6) is obtained carries out breeding plantation, need to be stored about 2 months Dormant period after, carry out Pregermination and seedling breeding under 30 DEG C of environment of temperature indoors, seedling can be moved inbred line population and planted to big by April in large bamboo hat with a conical crown and broad brim year Field.
Embodiment 3
A kind of efficient construction method of cassava inbred line population material, includes the following steps:
(1) in the torrid zone and south subtropics monsoon region, the sandy soil of the deep loosing soil of soil layer, level land or mountain selection of land: are selected Ground, planting site irrigation and drainage are convenient and 5 kilometer range of periphery is interior plants without other cassavas;
(2) it levels land and is applied with base manure: according to the line-spacing ridging of 1.0m, the kind plant hole that spacing in the rows is 1.2m is set on ridge; Compound potassium sulfate fertilizer, calcium magnesium phosphate, organic fertilizer are spread fertilizer over the fields in described kind of plant hole;The amount of application of the compound potassium sulfate fertilizer is 100kg/ hm2, the amount of application of the calcium magnesium phosphate is 400kg/hm2, the amount of application of the organic fertilizer is 600kgm2;The potassium sulfate is compound In fertilizer, N mass percentage 15%, P2O5Mass percentage 15%, K2O mass percentage 15%, the calcium magnesium phosphate In, P2O5Mass percentage 15%, MgO mass percentage 8%, CaO mass percentage 12%.The organic fertilizer has matter Amount point for addition microbial bacteria fermentation after the sheep dung of 30:30:30:10, cow dung, paulownia bran, phosphate fertilizer mixing than being made, the organic fertilizer In, quality of organic matter percentage composition 50%, N P and K mass percentage 6%;The microbial bacteria is by cellulose-decomposing bacterium, shallow lake Powder decomposer, lichengermium, nitrogen-fixing bacteria, EM bacterium, bacillus thuringiensis composition;
(3) sow: in mid or late March to early April, interlacing is staggered 10 days and plants, and the seedling stem oblique cutting of varieties of plant is broadcast Kind is in kind of plant hole;It is to start counting from the side of cassava planting site that the interlacing, which is staggered 10 days and plants specific method, odd-numbered line Cassava is planted on the same day, and even number line cassava is planted on the same day;Implantation time between odd-numbered line and even number line differs 10 days;
(4) promote Hua Baohua: in early June to late July, using flower forcing fertilizer;The method of application of the flower forcing fertilizer is root application Flower forcing solid fertilizer, the flower forcing solid fertilizer amount of application are 30kg/hm2, it is spaced application in 5 days once, sprays 2 times, full blossom stopping is applied With;Field management is paid attention to later, protects bud, is spaced 5 days root application guarantor fertilizers for potted flowers 1 time after deactivating flower forcing fertilizer;Guarantor's fertilizers for potted flowers application Amount is 50kg/hm2;Guarantor's fertilizers for potted flowers is alga fertilizer;In the alga fertilizer, alginic acid mass percentage is 48%.It is described In flower forcing solid fertilizer, N+P2O5+K2O gross mass percentage composition 5%, middle microelement 8%, quality of organic matter percentage composition 20%, PH value 6.5;Wherein, the middle microelement by mass ratio be the calcium of 12:0.1:0.1:0.1:0.1:0.2, magnesium, boron, iron, selenium, Zn-ef ficiency composition;
(5) pollinate: can completely by strictly open spontaneous pollination, can also by artificial supplementary pollination, as a result after can bagging Protection prevents from being scattered after seed is mature;5 days root application fruit retention fertilizer after cassava pollination;The dose of the fruit retention fertilizer is 100kg/ hm2;In the fruit retention fertilizer, humic acid 45g/L, organic matter 120g/L, N155g/L, P2O5172g/L、K2O195g/L,B15g/L;
(6) it harvests seed: mature selfed seed can be harvested successively January in November to next year and in 5 DEG C of temperature, humidity It is saved in 60% Freezing room;
(7) it nursery: when the inbred line population material that step (6) is obtained carries out breeding plantation, need to be stored about 2 months Dormant period after, carry out Pregermination and seedling breeding under 35 DEG C of environment of temperature indoors, seedling can be moved inbred line population and planted to big by April in large bamboo hat with a conical crown and broad brim year Field.
Embodiment 4
A kind of efficient construction method of cassava inbred line population material, includes the following steps:
(1) in the torrid zone and south subtropics monsoon region, the sandy soil of the deep loosing soil of soil layer, level land or mountain selection of land: are selected Ground, planting site irrigation and drainage are convenient and 5 kilometer range of periphery is interior plants without other cassavas;
(2) it levels land and is applied with base manure: according to the line-spacing ridging of 1.1m, the kind plant hole that spacing in the rows is 1.1m is set on ridge; Compound potassium sulfate fertilizer, calcium magnesium phosphate, organic fertilizer are spread fertilizer over the fields in described kind of plant hole;The amount of application of the compound potassium sulfate fertilizer is 200kg/ hm2, the amount of application of the calcium magnesium phosphate is 300kg/hm2, the amount of application of the organic fertilizer is 700kgm2, the potassium sulfate is compound In fertilizer, N mass percentage 15%, P2O5Mass percentage 15%, K2O mass percentage 15%, the calcium magnesium phosphate In, P2O5Mass percentage 18%, MgO mass percentage 8%, CaO mass percentage 24%.The organic fertilizer by etc. The fermentation of addition microbial bacteria is made after the sheep dung of mass ratio, cow dung, paulownia bran, phosphate fertilizer mix, and the microbial bacteria is by cellulose point Bacterium, starch decomposer, lichengermium, nitrogen-fixing bacteria, EM bacterium, bacillus thuringiensis is solved to form, in the organic fertilizer, organic matter Mass percentage 60%, N P and K mass percentage 8%.
(3) sow: in mid or late March to early April, interlacing is staggered 8 days and plants, and the seedling stem of varieties of plant is laid flat sowing In in kind of plant hole;It is to start counting from the side of cassava planting site that the interlacing, which is staggered 8 days and plants specific method, odd-numbered line cassava It plants on the same day, even number line cassava is planted on the same day;Implantation time between odd-numbered line and even number line differs 8 days;
(4) promote Hua Baohua: in early June to late July, using flower forcing fertilizer;The method of application of the flower forcing fertilizer is root application Flower forcing solid fertilizer, the flower forcing solid fertilizer amount of application are 80kg/hm2, it is spaced application in 9 days once, sprays 2 times, full blossom stopping is applied With;Pay attention to field management later, protect bud, deactivates and be spaced after flower forcing fertilizer potassium dihydrogen phosphate 1 time that sprays within 6 days 0.125%.Institute It states in flower forcing solid fertilizer, N+P2O5+K2O gross mass percentage composition 10%, middle microelement 10%, quality of organic matter percentage composition 15%, pH value 5.5;Wherein, the middle microelement by mass ratio be the calcium of 10:0.5:0.01:0.3:0.01:0.5, magnesium, boron, Iron, selenium, Zn-ef ficiency composition;
(5) pollinate: can completely by strictly open spontaneous pollination, can also by artificial supplementary pollination, as a result after can bagging Protection prevents from being scattered after seed is mature;12 days root application fruit retention fertilizer after cassava pollination;The dose of the fruit retention fertilizer is 60kg/ hm2;In the fruit retention fertilizer, humic acid 50g/L, organic matter 115g/L, N155g/L, P2O5165g/L、K2O195g/L,B22g/L;
(6) it harvests seed: mature selfed seed can be harvested successively January in November to next year and in 6 DEG C of temperature, humidity It is saved in 60% Freezing room.
(7) it nursery: when the inbred line population material that step (6) is obtained carries out breeding plantation, need to be stored about 2 months Dormant period after, carry out Pregermination and seedling breeding under 32 DEG C of environment of temperature indoors, seedling can be moved inbred line population and planted to big by April in large bamboo hat with a conical crown and broad brim year Field.
Planting experiment 1:
The selection Nanning Wuming experimental plot Qu Lijian is planting site, selects 048, Nan Zhi 199, SC9, ST-1 tetra- using new A kind plants breeding according to the method for 1~embodiment of embodiment 4, breeding statistical result such as the following table 1:
Table 1
Remarks: first time flowering time is the female time spent occur for the first time, similarly hereinafter.
Planting experiment 2:
Selection Guangxi Chongzuo City Longzhou County experimental plot is planting site, selects tetra- 048, Nan Zhi 199, SC9, ST-1 product using new Kind plants breeding according to the method for 1~embodiment of embodiment 4, breeding statistical result such as the following table 2:
Table 2
Planting experiment 3:
The selection Sanya, Hainan city town Jiu Suo experimental plot is planting site, selects tetra- 048, Nan Zhi 199, SC9, ST-1 product using new Kind plants breeding according to the method for 1~embodiment of embodiment 4, breeding statistical result such as the following table 3:
Table 3
Check experiment 1:
The selection Nanning Wuming experimental plot Qu Lijian is planting site, selects 048, Nan Zhi 199, SC9, ST-1 tetra- using new A kind is open in crop field and interlacing interval is not used to plant for 5~10 days, without promoting flower flower and fruit protecting (i.e. without step (4) promote flower and protect flower processing, step (5) does not apply fruit retention fertilizer), in the case where not using artificial hybridization etc. to handle, breeding and cultivation is obtained The result arrived such as the following table 4:
Table 4
By in upper table 1 and table 4, first time flowering time and seed start maturation time and compare the cassava it is found that of the invention Breeding method cassava does sth. in advance to bloom for 1 month, and cassava fruit is done sth. in advance to harvest for about 40 days, and seed is made to send out prenatal development in weathers such as cold damages Maturation shortens seed maturation time, can be transplanted to crop field in time after also ensuring second year seed seeding and seedling raising, shorten and educate The kind period.It is compared with insemination and emergence rate in table 4 it is found that the seed insemination and emergence rate phase that 4 Cassava Cultivars of the invention obtain by table 1 It is higher by 40% or more than control group, illustrate that seed fruit quality of the invention is high, the good emergence rate of quality is high.By single in table 1 and table 4 Strain inflorescence number compares with per plant number it is found that the per plant number of four Cassava Cultivars is compared in breeding method of the invention It is improved in each kind of control group, and newly selects the single plant inflorescence number of 048, Nan Zhi, 199 two kinds compared to the control group Height illustrates that the present invention can promote the difficult kind bloomed and bloom, and the wide in variety of Yi Kaihua blooms, and improves cassava into flower quantity and knot Real rate promotes breeding efficiency.
Check experiment 2:
Selection Guangxi Chongzuo City Longzhou County experimental plot is planting site, selects tetra- 048, Nan Zhi 199, SC9, ST-1 product using new Kind is open in crop field and interlacing interval is not used to plant for 5~10 days, without promoting flower flower and fruit protecting (i.e. without step (4) rush Hua Baohua processing, step (5) do not apply fruit retention fertilizer), in the case where not using artificial hybridization etc. to handle, knot that breeding and cultivation obtains Fruit such as the following table 5:
Table 5
By table 2, compared with table 5 it is found that using the method for the present invention, pollination to seed maturation time foreshortens to 90 days left sides It is right.The method of the present invention is not used, general pollination to seed maturation time needs 100-110 days or so.By table 2 and broadcasting in table 5 Kind of emergence rate is compared it is found that the obtained seed insemination and emergence rate of 4 Cassava Cultivars of the invention is high by 20% or more compared to the control group, The seed fruit quality for illustrating that the method for the present invention obtains is high, and quality is good, and emergence rate is high.By the per plant number in table 2 and table 5 Compared to it is found that the per plant number of the method for the present invention four strains is compared to control group height, illustrating that the present invention violates the law be can be improved Pollination rate and setting percentage.
Check experiment 3:
The selection Sanya, Hainan city town Jiu Suo experimental plot is planting site, selects tetra- 048, Nan Zhi 199, SC9, ST-1 product using new Kind is open in crop field and interlacing interval is not used to plant for 5~10 days, without promoting flower flower and fruit protecting (i.e. without step (4) rush Hua Baohua processing, step (5) do not apply fruit retention fertilizer), in the case where not using artificial hybridization etc. to handle, knot that breeding and cultivation obtains Fruit such as the following table 6:
Table 6
By table 3 it is found that pollination to seed maturation time foreshortens to 90 days or so using the method for the present invention compared with table 6. The method of the present invention is not used, general pollination to seed maturation time needs 100-110 days or so.Gone out by table 3 and the sowing in table 6 Seedling rate, single plant inflorescence number, per plant number are compared it is found that the method for the present invention is compared to control group, the single plant of 4 Cassava Cultivars Inflorescence number amplitude improves 3, and per plant number integrally doubles, and obtained seed insemination and emergence rate is high compared to the control group 10% or more, illustrate that quantity of blooming can be improved in the method for the present invention, Accelerate bloom, pollination rate is high, and setting percentage is high, the seed fruit of acquisition Real mass is high, and quality is good, and emergence rate is high.
By three local trial results of table 1 and table 4, table 2 and table 5, table 3 and table 6 it is found that the method for the present invention solves The torrid areas cassava florescence, cassava pollination rate was low vulnerable to typhoon influence, and it is low to directly result in cassava seed acquisition amount, subtropical zone monsoon Area, cassava fruit are difficult normal mature, and fruit development is abnormal or seed is of poor quality, along with cassava is cross pollinated plant, Same inflorescence female flower male flower flowering time has interval, and the pollination rate of breeding is extremely low, and fruit-setting rate is extremely low, and inbred line population material is difficult to The technical issues of efficiently obtaining.The method of the present invention can make cassava Blooming, and cassava is promoted to bloom, and improve cassava pollination rate and knot Real rate, obtained cassava seed quality is good, and emergence rate is high.
Above description is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair Bright patent claim, it is all the present invention suggested by technical spirit under completed same changes or modifications change, should all belong to In the covered the scope of the patents of the present invention.

Claims (10)

1. a kind of efficient construction method of cassava inbred line population material, which comprises the steps of:
(1) in the torrid zone and south subtropics monsoon region, the sandy soil of the deep loosing soil of soil layer, level land or mountainous region, kind selection of land: are selected It plants ground irrigation and drainage conveniently and is planted in 5 kilometer range of periphery without other cassavas;
(2) it levels land and is applied with base manure: according to the line-spacing ridging of 1.0~1.2m, the kind that spacing in the rows is 1.0~1.2m is set on ridge Plant hole;Compound potassium sulfate fertilizer, calcium magnesium phosphate, organic fertilizer are spread fertilizer over the fields in described kind of plant hole;
(3) sow: in mid or late March to early April, interlacing is staggered 5~10 days and plants, and the seedling stem of varieties of plant is laid flat or tiltedly It inserts and is seeded in kind of plant hole;
(4) promote Hua Baohua: in early June to late July, using flower forcing fertilizer;The method of application of the flower forcing fertilizer can be to spray to urge Flower Liquid Fertilizer or root application flower forcing solid fertilizer, the flower forcing solid fertilizer amount of application are 30~80kg/hm2, it is spaced application one in 7~10 days Secondary, full blossom stops application;Florescence pays attention to field management, protects bud, and root application protects fertilizers for potted flowers or sprays 0.075~0.125% Potassium dihydrogen phosphate;
(5) pollinate: can completely by strictly open spontaneous pollination, can also by artificial supplementary pollination, as a result after can protected by bagging, It prevents from being scattered after seed is mature;5~15 days root application fruit retention fertilizer after cassava pollination;The dose of the fruit retention fertilizer is 50~100kg/ hm2
(6) it harvests seed: mature selfed seed can be harvested successively January in November to next year and in 5 DEG C of temperature~10 DEG C, humidity It is saved in 60% Freezing room.
2. a kind of efficient construction method of cassava inbred line population material according to claim 1, which is characterized in that described to urge Flower Liquid Fertilizer in, mineral humic acid >=50g/L, EDTA- iron >=0.5g/L, EDTA- copper >=0.5g/L, potassium fulvate >=14g/L, Potassium sulfate >=5g/L, magnesium sulfate >=5g/L, potassium dihydrogen phosphate >=10g/L, dipotassium hydrogen phosphate >=5g/L.
3. a kind of efficient construction method of cassava inbred line population material according to claim 1, which is characterized in that described to urge In flower solid fertilizer, N+P2O5+K2O gross mass percentage composition >=5%, middle microelement >=8%, quality of organic matter percentage composition >= 15%, pH value 5.5~6.5.
4. according to a kind of efficient construction method of cassava inbred line population material described in claim 1, which is characterized in that the fruit retention In fertilizer, humic acid >=40g/L, organic matter >=110g/L, N >=150g/L, P2O5≥160g/L、K2O >=190g/L, B:15~ 25g/L。
5. a kind of efficient construction method of cassava inbred line population material according to claim 1, which is characterized in that the guarantor Fertilizers for potted flowers is alga fertilizer, in the alga fertilizer, alginic acid mass percentage >=36%, guarantor's fertilizers for potted flowers amount of application be 50~ 100kg/hm2
6. a kind of efficient construction method of cassava inbred line population material according to claim 1, which is characterized in that the sulphur The amount of application of sour K composite fertilizer is 100~300kg/hm2, the amount of application of the calcium magnesium phosphate is 200~400kg/hm2, described to have The amount of application of machine fertilizer is 600~1000kg/hm2
7. a kind of efficient construction method of cassava inbred line population material according to claim 6, which is characterized in that described In calcium magnesium phosphate, P2O5>=12%, MgO >=4%, CaO >=10%.
8. a kind of efficient construction method of cassava inbred line population material according to claim 6, which is characterized in that described to have Machine fertilizer is made of addition microbial bacteria fermentation after sheep dung, cow dung, paulownia bran, phosphate fertilizer mixing;In the organic fertilizer, quality of organic matter hundred Point content >=45%, N P and K mass percentage >=5%, the microbial bacteria by cellulose-decomposing bacterium, starch decomposer, Clothing Bacillus, nitrogen-fixing bacteria, EM bacterium, bacillus thuringiensis composition.
9. a kind of efficient construction method of cassava inbred line population material according to claim 1, which is characterized in that step (3) in sowing, the interlacing is staggered 5~10 days implantation methods are as follows: starts counting from the side of cassava planting site, odd-numbered line cassava It plants on the same day, even number line cassava is planted on the same day;Implantation time between odd-numbered line and even number line differs 5~10 days.
10. a kind of efficient construction method of cassava inbred line population material according to claim 1, which is characterized in that described The seed that step (6) obtains is nursery at 25~35 DEG C in room temperature.
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