CN110679423B - Cultivation method for reducing hydrocyanic acid content of cassava - Google Patents
Cultivation method for reducing hydrocyanic acid content of cassava Download PDFInfo
- Publication number
- CN110679423B CN110679423B CN201911091954.7A CN201911091954A CN110679423B CN 110679423 B CN110679423 B CN 110679423B CN 201911091954 A CN201911091954 A CN 201911091954A CN 110679423 B CN110679423 B CN 110679423B
- Authority
- CN
- China
- Prior art keywords
- variety
- cassava
- grafting
- scion
- planting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- LELOWRISYMNNSU-UHFFFAOYSA-N hydrogen cyanide Chemical compound N#C LELOWRISYMNNSU-UHFFFAOYSA-N 0.000 title claims abstract description 92
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 title claims abstract description 90
- 238000012364 cultivation method Methods 0.000 title claims abstract description 23
- 241000658379 Manihot esculenta subsp. esculenta Species 0.000 title 1
- 240000003183 Manihot esculenta Species 0.000 claims abstract description 93
- 239000003337 fertilizer Substances 0.000 claims abstract description 50
- 238000000034 method Methods 0.000 claims abstract description 33
- 238000003306 harvesting Methods 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 32
- 239000002689 soil Substances 0.000 claims description 31
- 241000196324 Embryophyta Species 0.000 claims description 24
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 20
- 241000607479 Yersinia pestis Species 0.000 claims description 15
- KMQAPZBMEMMKSS-UHFFFAOYSA-K calcium;magnesium;phosphate Chemical compound [Mg+2].[Ca+2].[O-]P([O-])([O-])=O KMQAPZBMEMMKSS-UHFFFAOYSA-K 0.000 claims description 14
- 201000010099 disease Diseases 0.000 claims description 14
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 14
- 239000003895 organic fertilizer Substances 0.000 claims description 13
- 235000010585 Ammi visnaga Nutrition 0.000 claims description 10
- 244000153158 Ammi visnaga Species 0.000 claims description 10
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 10
- 239000004202 carbamide Substances 0.000 claims description 10
- 235000011164 potassium chloride Nutrition 0.000 claims description 10
- 239000001103 potassium chloride Substances 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 9
- 238000003973 irrigation Methods 0.000 claims description 8
- 230000002262 irrigation Effects 0.000 claims description 8
- 244000061456 Solanum tuberosum Species 0.000 claims description 7
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 7
- 238000009825 accumulation Methods 0.000 claims description 5
- 230000000295 complement effect Effects 0.000 claims description 5
- 230000000694 effects Effects 0.000 abstract description 7
- 238000005516 engineering process Methods 0.000 abstract description 6
- 238000007726 management method Methods 0.000 description 28
- 238000011282 treatment Methods 0.000 description 16
- 241000238631 Hexapoda Species 0.000 description 12
- 238000002360 preparation method Methods 0.000 description 10
- 229920002472 Starch Polymers 0.000 description 9
- 230000004720 fertilization Effects 0.000 description 9
- 235000019698 starch Nutrition 0.000 description 9
- 239000008107 starch Substances 0.000 description 9
- 230000002265 prevention Effects 0.000 description 7
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 6
- 238000009331 sowing Methods 0.000 description 6
- 238000003971 tillage Methods 0.000 description 6
- 230000009286 beneficial effect Effects 0.000 description 5
- 235000013305 food Nutrition 0.000 description 5
- 239000011259 mixed solution Substances 0.000 description 5
- 238000002791 soaking Methods 0.000 description 5
- 235000017060 Arachis glabrata Nutrition 0.000 description 4
- 244000105624 Arachis hypogaea Species 0.000 description 4
- 235000010777 Arachis hypogaea Nutrition 0.000 description 4
- 235000018262 Arachis monticola Nutrition 0.000 description 4
- 241000287828 Gallus gallus Species 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 4
- 210000003608 fece Anatomy 0.000 description 4
- 239000010871 livestock manure Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 235000012054 meals Nutrition 0.000 description 4
- 235000020232 peanut Nutrition 0.000 description 4
- 239000010902 straw Substances 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 3
- 238000007605 air drying Methods 0.000 description 3
- 239000006013 carbendazim Substances 0.000 description 3
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000010899 nucleation Methods 0.000 description 3
- OXNIZHLAWKMVMX-UHFFFAOYSA-N picric acid Chemical compound OC1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O OXNIZHLAWKMVMX-UHFFFAOYSA-N 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000004563 wettable powder Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 244000299790 Rheum rhabarbarum Species 0.000 description 2
- 235000009411 Rheum rhabarbarum Nutrition 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 231100000053 low toxicity Toxicity 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 239000003440 toxic substance Substances 0.000 description 2
- 238000009333 weeding Methods 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000221017 Euphorbiaceae Species 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 235000004456 Manihot esculenta Nutrition 0.000 description 1
- 244000149260 Manihot esculenta subsp flabellifolia Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 230000002745 absorbent Effects 0.000 description 1
- 239000002250 absorbent Substances 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 235000021466 carotenoid Nutrition 0.000 description 1
- 150000001747 carotenoids Chemical class 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000000515 cyanogenic effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- 150000008131 glucosides Chemical class 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 235000005739 manihot Nutrition 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000011369 optimal treatment Methods 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 229940075930 picrate Drugs 0.000 description 1
- OXNIZHLAWKMVMX-UHFFFAOYSA-M picrate anion Chemical compound [O-]C1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O OXNIZHLAWKMVMX-UHFFFAOYSA-M 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000010025 steaming Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
- A01B79/02—Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Mechanical Engineering (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Cultivation Of Plants (AREA)
- Fertilizers (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a cultivation method for reducing the hydrocyanic acid content of cassava, which comprises the following steps: (1) variety selection: selecting a newly selected 048 or Y22 cassava variety as a stock variety, and selecting a No. 9 Huanan variety or a P9 cassava variety as a scion variety; (2) planting and grafting: respectively planting and managing the stock varieties and the scion varieties; planting for 1.5-2 months, and grafting the selected scions to the planted stocks by adopting a branch grafting method when the seedlings are 60-100 cm high; (3) and (5) managing and harvesting. The invention achieves the technical effect of reducing the content of the hydrocyanic acid in the root stock variety by the grafting technology, the optimal fertilizer proportion, the moisture management technology and the like.
Description
Technical Field
The invention relates to the technical field of agricultural planting, in particular to a cultivation method for reducing the hydrocyanic acid content of cassava.
Background
Cassava (Manihot esculenta Crantz) is a plant of the genus Manihot of the family Euphorbiaceae, the third food crop in the hot zone, is widely cultivated in more than 100 countries or regions such as Africa, America and Asia, and is a food on which nearly six hundred million people live in the world. In recent years, along with the improvement of living standard of people, the cassava has more diversified and functional requirements on material life, and the cassava serving as a special potato coarse cereal is brand-new in the food industry.
Cassava roots contain a substance called cyanogenic glucoside, which can decompose and release a toxic substance HCN, and when the cassava roots are eaten, great care needs to be taken, poisoning can happen if the cassava roots are careless, and partial varieties need to be soaked in clear water before being eaten. Studies show that the removal effect of steaming, roasting and frying is worse when the cassava is boiled for 30 minutes, and only 50-55% of HCN content in tuberous roots can be removed. At present, toxic substances HCN in cassava are mainly removed by processing after being harvested, and the HCN content in the cassava is reduced by a cultivation technical method, so that no relevant research report is found at present. Some new cassava varieties have high yield, good quality and good plant type, but the HCN content is higher, so the cassava varieties are not suitable for eating, and the high-valued development and utilization of the cassava varieties are not facilitated. Therefore, the reduction of the content of HCN in the cassava root tubers by the cultivation technical method has important significance for the development and utilization of cassava food and the development of cassava industry.
Disclosure of Invention
In order to solve the problems, the invention provides a cultivation method for reducing the hydrocyanic acid content of cassava. The hydrocyanic acid content in the tuberous roots of the excellent new variety is remarkably reduced by the grafting technology and the cultivation technology, so that the quality of the cassava tuberous roots is improved.
In order to solve the problems, the invention adopts the following technical scheme:
a cultivation method for reducing the hydrocyanic acid content of cassava comprises the following steps:
(1) variety selection: selecting a variety A as a stock variety and selecting a variety B as a scion variety; the variety A is a newly selected 048 variety or strain Y22; the variety B is No. 9 of south China or P9;
(2) planting and grafting: respectively planting and managing the stock varieties and the scion varieties; planting for 1.5-2 months, and grafting the selected scions to the planted stocks by adopting a branch grafting method when the seedlings are 60-100 cm high; the grafting by the grafting method comprises the following steps:
a. selecting a strongly growing twig as a scion, cutting a stem section with 2-3 intact bud eyes and a length of about 5-8 cm as the scion, cutting the upper part of the scion into a plane, cutting the lower part of the scion into an inclined plane of about 25-40 degrees, and increasing the contact surface;
b. selecting plants with the same maturity degree and the same thickness as the scion as stocks, cutting upper branches at the parts with the same thickness as the scion, and cutting the cuts into slopes complementary to the scion cuts;
c. one end of a toothpick is inserted into the central medullary part of the scion, the other end of the toothpick is inserted into the central medullary part of the stock, so that 2 inclined planes are closely matched, the grafting part is tightly bound and firmly bound by a plastic binding band, and meanwhile, the upper plane of the scion is wrapped and sealed;
d. after 20-30 days of grafting, the scion grows normally, and the binding belt can be removed as required;
(3) and (5) managing and harvesting.
Wherein the scion variety is No. 9 variety of south China or P9 variety.
Among them, the variety No. 9 of south China, which was introduced from research institute of tropical crop variety resources of the institute of tropical agriculture academy of sciences, has the variety registration number XPD 012-2012.
Wherein, the P9 is a good edible cassava strain containing high carotenoid and low hydrocyanic acid obtained by the research institute of economic crops of Guangxi Zhuang autonomous region agricultural academy of sciences through hybridization breeding.
Wherein the stock variety is a newly selected 048 variety or strain Y22.
Wherein, a 048 variety is newly selected, an excellent single plant is selected from a cassava germplasm resource ZM93-16 natural variation population, and the excellent single plant is bred through systematic breeding and directional selection. Variety approval serial number: guiju potato No. 2006001.
The strain Y22 is a cassava variety imported from the International cassava research center and derived from Peru edible cassava, and the variety number is PER 183.
In the water and fertilizer management, a fertilizer application method comprises the following steps: before cassava planting, planting furrows are formed on the ridge surfaces, and the fertilizer formula and the using amount in the furrows are as follows: 350-500 kg/mu of organic fertilizer, 10-15 kg/mu of urea, 20-30 kg/mu of calcium magnesium phosphate fertilizer and 20-35 kg/mu of potassium chloride; the fertilizer and the soil for planting the holes are uniformly mixed according to the mass ratio of 1: 10-15. The organic fertilizer is prepared from human and animal excrement, chicken manure, straw, plant ash and peanut meal according to a mass ratio of 100:20:10: 30: 20, mixing and stacking for 2-3 months. In the calcium magnesium phosphate fertilizer, P2O5 is more than or equal to 12 percent, MgO is more than or equal to 4 percent, and CaO is more than or equal to 10 percent.
Wherein, in the water and fertilizer management, the water management comprises the following steps: and (3) installing a sprinkling irrigation or dropper facility in the field, keeping the field moist in the seedling stage, keeping the relative humidity of soil at 60-80% in the potato bearing stage, and draining water in rainy seasons to prevent water accumulation.
In the cultivation method, the pH value of soil for planting cassava is 5.0-6.5. The soil condition within the pH value range is favorable for reducing the content of hydrocyanic acid in cassava.
In the invention, the management and harvesting method in the step (3) is used for carrying out water and fertilizer management and disease, insect and weed control according to the edible cassava high-yield cultivation method. Wherein the water content management is as follows: when the soil cassava is drought-enduring, watering is carried out appropriately, waterlogging needs to be drained in time in rainy seasons, and soil moisture is wet and dry in water and fertilizer management. The fertilization management comprises the following steps: the top application can be carried out without top application or once, and 20 kg/mu compound fertilizer is applied as the top application one month after grafting and in rainy days. The pest control is as follows: the prevention is mainly adopted, the comprehensive prevention and control is taken as a principle, when the diseases and insect pests do not occur in a large area, firstly, a manual pulling-out mode is adopted, the diseases and insect pests are destroyed in time, secondly, ecological conditions which are not beneficial to the occurrence of the diseases and insect pests but are beneficial to or do not hinder the growth of cassava are created through measures such as weeding, hilling and the like, the areas of the diseases and insect pests are large, and high-efficiency, low-toxicity and low-residue pesticide varieties are selected for prevention and control.
Compared with the prior art, the invention has the following beneficial effects:
(1) the method adopts a newly selected 048 variety and a strain Y22 variety as the stock for planting, selects a No. 9 variety or a P9 variety as a scion for grafting at a proper time, and achieves the purposes of reducing the root tuber hydrocyanic acid content of the stock variety and ensuring the yield and the starch content through an optimal fertilizer ratio, a water management technology and the like. By the method, newly selected 048 is used as a rootstock, No. 9 of south China is used as the scion, the yield of the root tuber is reduced by a small margin compared with a control (rootstock), the reduction margin is lower than 9.3 percent, the starch content is increased by more than 0.7 percent compared with the control, and the HCN content is reduced to 20-30mg/kg from 100-200 mg/kg; by using the newly selected 048 as the rootstock and the P9 as the scion, the root tuber yield is reduced by 3.0 percent compared with the control (rootstock), the starch content is increased by 0.3 percent compared with the control, and the HCN content is reduced to 30-50mg/kg from 100-200 mg/kg; by using the strain Y22 as the rootstock and using No. 9 of south China as the scion, the root tuber yield is reduced by 1.5 percent compared with the control (rootstock), the starch content is reduced by 0.9 percent compared with the control, and the HCN content is reduced to 50-100mg/kg from 200-400 mg/kg. By using the strain Y22 as the rootstock and P9 as the scion, the tuberous root yield is reduced to a small extent compared with the control (rootstock), the reduction extent is less than 8.2 percent, the starch content is reduced by less than 2.7 percent compared with the control, and the HCN content is reduced to 50-100mg/kg from 200-400 mg/kg.
(2) Cassava can grow in the soil pH value range of 4.0-7.5, however, when the cassava cultivation method is carried out in the soil pH value range of 5.0-6.5, nitrogen, sulfur, phosphorus, potassium, magnesium, zinc, calcium and organic matters with appropriate content in a proper proportion can be formed in the soil, the activity of the biological enzyme in the soil is improved, and the content of cassava hydrocyanic acid can be effectively reduced.
Detailed Description
The present invention will be further described with reference to examples and tests.
Example 1
A cultivation method for reducing the hydrocyanic acid content of cassava comprises the following steps:
(1) selecting and preparing land
Selecting land: selecting a land block or a gentle slope land with a deep soil layer and convenient irrigation and drainage. The soil is sandy loam with pH 6.0.
Land preparation: the land preparation is suitable for machine tillage, the tillage depth is 35cm, and the deep, loose, fine, broken, flat and weed-free effects are achieved. Ridging is carried out according to the row spacing of the planted plants, and planting can be carried out in one ridge and two rows or in one ridge and one row. The ridges are 12cm high. Drainage ditches are arranged around the water tank.
(2) Variety selection
The cassava variety 'newly selected 048' is selected as a stock variety, and the cassava variety 'No. 9' is selected as a scion variety.
(3) Planting and grafting
Seed selection and seed stem treatment: mature seed stems which are thick, short in internodes, complete in stem bark, strong in bud eyes and free of diseases and insect pests are selected as seeding materials. The seed stems were cut into 18cm long stem sections before sowing. Soaking in 1000 times of 50% carbendazim wettable powder for 4min, taking out, air drying, and inoculating.
Sowing: and planting in the middle and last ten days of 3 months to the last ten days of 4 months in a flat or oblique inserting mode. The row spacing of the plants is 0.8m by 0.8 m.
Thinning: after one month of seedling emergence, thinning is carried out, and 1 robust seedling is reserved in each planting hole.
Fertilization management: before cassava planting, planting furrows are formed on the ridge surfaces, and the fertilizer formula and the using amount in the furrows are as follows: 500 kg/mu of organic fertilizer, 10 kg/mu of urea, 30 kg/mu of calcium magnesium phosphate fertilizer and 20 kg/mu of potassium chloride; and uniformly mixing the fertilizer and the soil of the planting hole according to the mass ratio of 1: 12. The organic fertilizer is prepared from human and animal excrement, chicken manure, straw, plant ash and peanut meal according to a mass ratio of 100:20:10: 30: 20, mixing and stacking for 2.5 months. Wherein, in the calcium magnesium phosphate fertilizer, P is2O5The weight percentage content of the composite material is 18 percent, the weight percentage content of MgO is 5 percent, and the weight percentage content of CaO is 15 percent.
Water content management: a sprinkling irrigation or burette facility is installed in the field, the field is kept moist in the seedling stage, the relative humidity of soil in the potato bearing stage is kept at 70%, and water is drained in rainy season to prevent water accumulation;
grafting: and (4) grafting the selected scions to the planted rootstocks by adopting a branch grafting method 1.5 months after planting and when the seedlings are 100cm high.
The grafting by the grafting method comprises the following steps:
a. selecting a strongly growing twig as a scion, cutting a stem segment with 3 intact bud eyes and a length of about 5cm as the scion, cutting the upper part of the scion into a plane, cutting the lower part of the scion into an inclined plane with an angle of about 25 degrees, and increasing the contact surface;
b. selecting plants with the same maturity degree and the same thickness as the scion as stocks, cutting upper branches at the parts with the same thickness as the scion, and cutting the cuts into slopes complementary to the scion cuts;
c. one end of a toothpick is inserted into the central medullary part of the scion, the other end of the toothpick is inserted into the central medullary part of the stock, so that 2 inclined planes are closely matched, the grafting part is tightly bound and firmly bound by a plastic binding band, and meanwhile, the upper plane of the scion is wrapped and sealed;
d. 30 days after grafting, the scion grows normally, and the binding belt can be removed as required;
(4) managing harvesting
The management and harvesting method is used for water and fertilizer management and prevention and control of diseases, pests and weeds according to the edible cassava high-yield cultivation method. Wherein the water content management is as follows: when the soil cassava is drought-enduring, watering is carried out appropriately, waterlogging needs to be drained in time in rainy seasons, and soil moisture is wet and dry in water and fertilizer management. The fertilization management comprises the following steps: no additional fertilizer is applied. The pest control is as follows: the prevention is mainly adopted, the comprehensive prevention and control is taken as a principle, when the diseases and insect pests do not occur in a large area, firstly, a manual pulling-out mode is adopted, and the diseases and insect pests are destroyed in time, secondly, ecological conditions which are not beneficial to the occurrence of the diseases and insect pests but are beneficial to or do not hinder the growth of the cassava are created through measures such as weeding, ridging and the like, the areas of the diseases and insect pests are large, and high-efficiency, low-toxicity and low-residue pesticide varieties are selected for prevention and control.
Example 2
A cultivation method for reducing the hydrocyanic acid content of cassava comprises the following steps:
(1) selecting and preparing land
Selecting land: selecting a land block or a gentle slope land with a deep soil layer and convenient irrigation and drainage. The soil is sandy loam with pH 6.5.
Land preparation: the land preparation is suitable for machine tillage, the tillage depth is 30cm, and the deep, loose, fine, broken, flat and weed-free effects are achieved. Ridging is carried out according to the row spacing of the planted plants, and planting can be carried out in one ridge and two rows or in one ridge and one row. The ridges are 15cm high. Drainage ditches are arranged around the water tank.
(2) Variety selection
The cassava variety 'strain Y22' is selected as a rootstock variety, and the cassava variety 'P9' is selected as a scion variety.
(3) Planting and grafting
Seed selection and seed stem treatment: mature seed stems which are thick, short in internodes, complete in stem bark, strong in bud eyes and free of diseases and insect pests are selected as seeding materials. The seed stems were cut into 15cm long stem sections before sowing. Soaking in 1000 times of 50% carbendazim wettable powder for 5min, taking out, air drying, and inoculating.
Sowing: and planting in the middle and last ten days of 3 months to the last ten days of 4 months in a flat or oblique inserting mode. The row spacing of the plants is 0.8m by 1.0 m.
Thinning: after one month of seedling emergence, thinning is carried out, and 1 robust seedling is reserved in each planting hole.
Fertilization management: before cassava planting, planting furrows are formed on the ridge surfaces, and the fertilizer formula and the using amount in the furrows are as follows: 350 kg/mu of organic fertilizer, 15 kg/mu of urea, 20 kg/mu of calcium magnesium phosphate fertilizer and 35 kg/mu of potassium chloride; and uniformly mixing the fertilizer and the soil in the planting hole according to the mass ratio of 1: 10. The organic fertilizer is prepared from human and animal excrement, chicken manure, straw, plant ash and peanut meal according to a mass ratio of 100:20:10: 30: 20 mixing and stacking for 2 months. Wherein, in the calcium magnesium phosphate fertilizer, P is2O515 percent of MgO, 12 percent of CaO and 15 percent of MgO.
Water content management: a sprinkling irrigation or burette facility is installed in the field, the field is kept moist in the seedling stage, the relative humidity of soil in the potato bearing stage is kept at 60%, and water is drained in rainy season to prevent water accumulation;
grafting: and 2 months after planting, and grafting the selected scions to the planted rootstocks by adopting a branch grafting method when the height of the seedlings is 60 cm.
The grafting by the grafting method comprises the following steps:
a. selecting a strongly growing twig as a scion, cutting a stem section with 2 intact bud eyes and a length of about 8cm as the scion, cutting the upper part of the scion into a plane, cutting the lower part of the scion into an inclined plane of about 40 degrees, and increasing the contact surface;
b. selecting plants with the same maturity degree and the same thickness as the scion as stocks, cutting upper branches at the parts with the same thickness as the scion, and cutting the cuts into slopes complementary to the scion cuts;
c. one end of a toothpick is inserted into the central medullary part of the scion, the other end of the toothpick is inserted into the central medullary part of the stock, so that 2 inclined planes are closely matched, the grafting part is tightly bound and firmly bound by a plastic binding band, and meanwhile, the upper plane of the scion is wrapped and sealed;
d. after 20 days of grafting, the scion grows normally, and the binding belt can be removed as required;
(4) managing harvesting
The field management method in the later stage of cassava plant grafting is the same as that in example 1.
Example 3
A cultivation method for reducing the hydrocyanic acid content of cassava comprises the following steps:
(1) selecting and preparing land
Selecting land: selecting a land block or a gentle slope land with a deep soil layer and convenient irrigation and drainage. The soil is sandy loam with pH 5.0.
Land preparation: the land preparation is suitable for machine tillage, the tillage depth is 40cm, and the deep, loose, fine, broken, flat and weed-free effects are achieved. Ridging is carried out according to the row spacing of the planted plants, and planting can be carried out in one ridge and two rows or in one ridge and one row. The ridges are 10cm high. Drainage ditches are arranged around the water tank.
(2) Variety selection
The cassava variety 'newly selected 048' is selected as a rootstock variety, and the cassava variety 'P9' is selected as a scion variety.
(3) Planting and grafting
Seed selection and seed stem treatment: mature seed stems which are thick, short in internodes, complete in stem bark, strong in bud eyes and free of diseases and insect pests are selected as seeding materials. The seed stems were cut into 20cm long stem sections before sowing. Soaking in 1000 times of 50% carbendazim wettable powder for 3min, taking out, air drying, and inoculating.
Sowing: and planting in the middle and last ten days of 3 months to the last ten days of 4 months in a flat or oblique inserting mode. The row spacing of the plants is 0.8m by 1.2 m.
Thinning: after one month of seedling emergence, thinning is carried out, and 1 robust seedling is reserved in each planting hole.
Fertilization management: before cassava planting, planting furrows are formed on the ridge surfaces, and the fertilizer formula and the using amount in the furrows are as follows: 400 kg/mu of organic fertilizer, 12 kg/mu of urea, 25 kg/mu of calcium magnesium phosphate fertilizer and 28 kg/mu of potassium chloride; and uniformly mixing the fertilizer and the soil in the planting hole according to the mass ratio of 1: 15. The organic fertilizer is prepared from human and animal excrement, chicken manure, straw, plant ash and peanut meal according to a mass ratio of 100:20:10: 30: 20 mixing and stacking for 3 months. Wherein, in the calcium magnesium phosphate fertilizer, P is2O512 percent of MgO, 4 percent of CaO and 10 percent of CaO.
Water content management: a sprinkling irrigation or burette facility is installed in the field, the field is kept moist in the seedling stage, the relative humidity of soil in the potato bearing stage is kept at 80%, and water is drained in rainy season to prevent water accumulation;
grafting: and (4) grafting the selected scions to the planted rootstocks by adopting a branch grafting method 55 days after planting when the seedlings are 80cm high.
The grafting by the grafting method comprises the following steps:
a. selecting a strongly growing twig as a scion, cutting a stem section with 23 intact bud eyes and a length of about 6cm as the scion, cutting the upper part of the scion into a plane, cutting the lower part of the scion into an inclined plane with an angle of about 35 degrees, and increasing the contact surface;
b. selecting plants with the same maturity degree and the same thickness as the scion as stocks, cutting upper branches at the parts with the same thickness as the scion, and cutting the cuts into slopes complementary to the scion cuts;
c. one end of a toothpick is inserted into the central medullary part of the scion, the other end of the toothpick is inserted into the central medullary part of the stock, so that 2 inclined planes are closely matched, the grafting part is tightly bound and firmly bound by a plastic binding band, and meanwhile, the upper plane of the scion is wrapped and sealed;
d. after 25 days of grafting, the scion grows normally, and the binding belt can be removed as required;
(4) managing harvesting
The field management method in the later stage of cassava plant grafting is the same as that in example 1.
Example 4
The cultivation method for reducing the hydrocyanic acid content of cassava in the embodiment 4 is basically the same as that in the embodiment 3, and is characterized in that in the variety selection, a cassava variety 'Y22' is selected as a stock variety, and a cassava variety 'Huanan No. 9' is selected as a scion variety.
Example 5
The cultivation method for reducing the hydrocyanic acid content of cassava in the embodiment 5 is basically the same as that of the embodiment 1, except that the fertilization method of the embodiment 3 is adopted.
Example 6
The cultivation method for reducing the hydrocyanic acid content of cassava in the embodiment 6 is basically the same as that of the embodiment 1, except that the fertilization method of the embodiment 3 is adopted.
Control group 1
Adopting a newly selected 048 as a control group to plant a variety, not carrying out grafting treatment on cassava, and carrying out land selection and soil preparation in other steps (1), planting in the step (3), grafting and management and harvesting in the step (4) which are the same as those in the embodiment 3.
Control group 2
And (3) adopting No. 9 of south China as a control group to plant a variety, not carrying out grafting treatment on cassava, and carrying out land selection and soil preparation, planting in the step (3), grafting and management and harvesting in the step (4) in the other steps which are the same as those in the embodiment 3.
Control group 3
And (3) adopting P9 as a control group for planting a variety, not carrying out grafting treatment on cassava, and carrying out land selection and soil preparation in other steps (1), planting in the step (3), grafting and management and harvesting in the step (4) which are the same as those in the embodiment 3.
Control group 4
And (3) adopting Y22 as a control group for planting a variety, not carrying out grafting treatment on cassava, and carrying out land selection and soil preparation in other steps (1), planting in the step (3), grafting and management and harvesting in the step (4) which are the same as those in the embodiment 3.
Experiment 1: influence of grafting technology on HCN content of cassava root tuber
The hydrocyanic acid (HCN) content in cassava roots (without bark, the same below) of examples 1 to 6 and control groups 1 to 4 was measured: and the starch content in the cassava root tubers of examples 1 to 6 and control groups 1 to 4 was determined with reference to the national standard GB 5009.9-2016 (determination of starch in national food safety standards), and the cassava yield was counted, the results are reported in Table 1 below.
The method for detecting the HCN content in the cassava root tubers comprises the following steps: taking equal volume of NaCO3Mixing with picric acid to obtain picric acid salt mixed solution, soaking filter paper strip in the picric acid salt mixed solution for soaking and timing, taking out the filter paper strip soaked with the mixed solution after 5min, and placing on absorbent paper to remove excessive mixed solution; weighing about 1g of small cassava block root sample, placing the small cassava block root sample at the bottom of a test tube, weighing 200uL of toluene, dripping the toluene on the cassava block root sample at the bottom of the test tube, placing a filter paper strip soaked with the picrate mixed solution at the opening of the test tube, sealing the filter paper strip with a rubber plug, placing the filter paper strip into an incubator for culture (the culture temperature is 28 ℃), and comparing and identifying the card according to a standard color degree ratio after the culture is finished to obtain the content of HCN in the cassava block root. Wherein, the chroma standard ratio is to-card (mg/kg):<10, bright yellow; 10-20, yellow or dark yellow; 20-30, dark yellow or sallow; 30-50 of yellow sand or rhubarb; 50-100 of rhubarb or khaki; 100-; 200-; 400-800 in autumn orange or red brown.
TABLE 1
As can be seen from Table 1, the characteristics of the root tuber harvested after grafting, the yield and the starch content tend to those of the stock variety, and the HCN content is significantly influenced by the scion variety. The method adopts the newly selected 048 and Y22 varieties as rootstocks for planting, adopts the scions of No. 9 and P9 varieties in south China for grafting treatment at proper time, can greatly reduce the HCN content in cassava tuberous roots, simultaneously ensures the cassava yield and the starch content, and shows from another angle that the method improves the yield of the edible cassava (the cassava with the HCN content of the fresh cassava tuberous roots lower than 50mg/kg is called sweet cassava internationally, namely the instant cassava) tuberous roots.
Experiment 2: influence of fertilizer management on HCN content of cassava root tuber
Variety treatment group:
number 1: and (3) selecting 048 newly, planting the cassava variety according to the planting method provided in example 1, wherein the difference is that grafting treatment is not carried out, and fertilizer application methods are respectively carried out according to fertilizer treatments of C1, C2, C3, C4, C5, C6 and C7.
Sequence number 2: south China No. 9, the planting method of the cassava variety is planting according to the planting method provided in example 1, and is different from the planting method that grafting treatment is not carried out, and fertilizer application methods are respectively carried out according to fertilizer treatment and fertilizer application methods of C1, C2, C3, C4, C5, C6 and C7.
Sequence No. 3: and selecting a 048 rootstock and a No. 9 scion from south China, and planting according to the cassava cultivation method in the embodiment 1. Except that the grafting treatment is not carried out, and the fertilization method is respectively carried out according to fertilizer treatments of C1, C2, C3, C4, C5, C6 and C7.
And (3) fertilizer treatment group:
c1: 500 kg/mu of organic fertilizer, 10 kg/mu of urea, 30 kg/mu of calcium magnesium phosphate fertilizer and 20 kg/mu of potassium chloride, namely the fertilizing conditions of the embodiment 1;
c2: 350 kg/mu of organic fertilizer, 15 kg/mu of urea, 20 kg/mu of calcium magnesium phosphate fertilizer and 35 kg/mu of potassium chloride, namely the fertilizing conditions of the embodiment 2;
c3: 400 kg/mu of organic fertilizer, 12 kg/mu of urea, 25 kg/mu of calcium magnesium phosphate fertilizer and 28 kg/mu of potassium chloride, namely the fertilizing conditions of the embodiments 3 to 6 and the control groups 1 to 4;
c4: 400 kg/mu of organic fertilizer, 28 kg/mu of urea, 12 kg/mu of calcium magnesium phosphate fertilizer and 28 kg/mu of potassium chloride;
c5: 12 kg/mu of urea, 25 kg/mu of calcium magnesium phosphate fertilizer and 28 kg/mu of potassium chloride;
c6: compound fertilizer (N: P)2O5:K2O15: 15:15)67 kg/mu;
c7: no fertilizer is applied;
the results of detecting the HCN content in the cassava root tubers of each experimental group according to the HCN content detection method of experiment 1 are shown in table 1 below:
table 2 HCN content units: mg/kg
The HCN content effect in Table 2 shows that C1, C2 and C3 are optimal treatments, namely in the fertilization management method, the fertilizer formula and the dosage are reasonable, and the HCN content in cassava root tubers is reduced.
The above description is intended to describe in detail the preferred embodiments of the present invention, but the embodiments are not intended to limit the scope of the claims of the present invention, and all equivalent changes and modifications made within the technical spirit of the present invention should fall within the scope of the claims of the present invention.
Claims (4)
1. A cultivation method for reducing the hydrocyanic acid content of cassava is characterized by comprising the following steps:
(1) variety selection: selecting a variety A as a stock variety and selecting a variety B as a scion variety; the variety A is a newly selected 048 variety or strain Y22; the variety B is No. 9 of south China or P9;
(2) planting and grafting: planting two cassava varieties respectively, and performing water and fertilizer management; planting for 1.5-2 months, and grafting the selected scions to the planted stocks by adopting a branch grafting method when the seedlings are 60-100 cm high; the grafting by the grafting method comprises the following steps:
a. selecting strongly growing twigs as scions, cutting stem segments with 2-3 intact bud eyes and a length of 5-8 cm as the scions, cutting the upper parts of the scions into planes, cutting the lower parts of the scions into inclined planes of 25-40 degrees, and increasing the contact surface;
b. selecting plants with the same maturity degree and the same thickness as the scion as stocks, cutting upper branches at the parts with the same thickness as the scion, and cutting the cuts into slopes complementary to the scion cuts;
c. one end of a toothpick is inserted into the central medullary part of the scion, the other end of the toothpick is inserted into the central medullary part of the stock, so that 2 inclined planes are closely matched, the grafting part is tightly bound and firmly bound by a plastic binding band, and meanwhile, the upper plane of the scion is wrapped and sealed;
d. after 20-30 days of grafting, the scion grows normally, and the binding belt is removed as required;
in the water and fertilizer management, the fertilizing method comprises the following steps: before cassava planting, planting furrows are formed on the ridge surfaces, and the fertilizer formula and the using amount in the furrows are as follows: 350-500 kg/mu of organic fertilizer, 10-15 kg/mu of urea, 20-30 kg/mu of calcium magnesium phosphate fertilizer and 20-35 kg/mu of potassium chloride; uniformly mixing the fertilizer with the soil in the planting hole;
in the water and fertilizer management, the water management comprises the following steps: a sprinkling irrigation or burette facility is installed in the field, the field is kept moist in the seedling stage, the relative humidity of soil in the potato bearing stage is kept at 60-80%, and water is drained in rainy season to prevent water accumulation;
(3) and (5) managing and harvesting.
2. The cultivation method for reducing the hydrocyanic acid content of cassava according to claim 1, wherein the variety A is a newly selected 048 variety; the variety B is No. 9 of south China.
3. The cultivation method for reducing the hydrocyanic acid content of cassava according to claim 1, wherein in the cultivation method, the pH value of soil for cassava planting is 5.0-6.5.
4. The cultivation method for reducing the hydrocyanic acid content of cassava according to claim 1, wherein the management and harvesting method in the step (3) is to perform water and fertilizer management and control of diseases, pests and weeds according to the edible cassava high-yield cultivation method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911091954.7A CN110679423B (en) | 2019-11-08 | 2019-11-08 | Cultivation method for reducing hydrocyanic acid content of cassava |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911091954.7A CN110679423B (en) | 2019-11-08 | 2019-11-08 | Cultivation method for reducing hydrocyanic acid content of cassava |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110679423A CN110679423A (en) | 2020-01-14 |
| CN110679423B true CN110679423B (en) | 2021-07-27 |
Family
ID=69116051
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911091954.7A Active CN110679423B (en) | 2019-11-08 | 2019-11-08 | Cultivation method for reducing hydrocyanic acid content of cassava |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110679423B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112913610A (en) * | 2021-01-26 | 2021-06-08 | 广西壮族自治区农业科学院 | Cultivation method for effectively reducing hydrocyanic acid content of cassava root tuber |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101103670A (en) * | 2007-08-07 | 2008-01-16 | 广西亚热带作物研究所 | Cultivating method for promoting cassava production |
| CN106576723A (en) * | 2016-11-09 | 2017-04-26 | 广西壮族自治区亚热带作物研究所 | Method of improving survival rate of cassava in drought and barren mountainous region |
| CN106954506A (en) * | 2017-03-01 | 2017-07-18 | 苏州梦龙文化旅游发展有限公司 | The implantation methods of cyanogen salidroside content in a kind of reduction cherry fruit |
| CN107212330A (en) * | 2017-05-17 | 2017-09-29 | 保山全心农业科技有限公司 | A kind of preparation method of cassava whole-powder noodles |
| CN107736211A (en) * | 2017-11-24 | 2018-02-27 | 藤县福进大红八角种植专业合作社 | A kind of implantation methods of cassava |
| CN110199809A (en) * | 2019-06-27 | 2019-09-06 | 广西壮族自治区农业科学院 | A kind of efficient construction method of cassava inbred line population material |
-
2019
- 2019-11-08 CN CN201911091954.7A patent/CN110679423B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101103670A (en) * | 2007-08-07 | 2008-01-16 | 广西亚热带作物研究所 | Cultivating method for promoting cassava production |
| CN106576723A (en) * | 2016-11-09 | 2017-04-26 | 广西壮族自治区亚热带作物研究所 | Method of improving survival rate of cassava in drought and barren mountainous region |
| CN106954506A (en) * | 2017-03-01 | 2017-07-18 | 苏州梦龙文化旅游发展有限公司 | The implantation methods of cyanogen salidroside content in a kind of reduction cherry fruit |
| CN107212330A (en) * | 2017-05-17 | 2017-09-29 | 保山全心农业科技有限公司 | A kind of preparation method of cassava whole-powder noodles |
| CN107736211A (en) * | 2017-11-24 | 2018-02-27 | 藤县福进大红八角种植专业合作社 | A kind of implantation methods of cassava |
| CN110199809A (en) * | 2019-06-27 | 2019-09-06 | 广西壮族自治区农业科学院 | A kind of efficient construction method of cassava inbred line population material |
Non-Patent Citations (4)
| Title |
|---|
| 3种处理方法对银杏种仁中氢氰酸和4-甲氧基吡哆醇质量分数的影响;花彤彤等;《东北林业大学学报》;20180509;第46卷(第06期);第38-42页 * |
| 中国木薯施肥研究进展;宋付平等;《中国农学通报》;20090220;第25卷(第04期);第140-144页 * |
| 江西红壤旱地木薯农艺性状与产量的关系初步研究;林洪鑫等;《中国农学通报》;20111025;第27卷(第27期);第204-208页 * |
| 良好操作规范的木薯栽培技术;许瑞丽等;《广东农业科学》;20090310(第03期);第39-42页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN110679423A (en) | 2020-01-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Belehu | Agronomical and physiological factors affecting growth, development and yield of sweet potato in Ethiopia | |
| CN109496734A (en) | Method and a kind of ferment rice using ferment rice cultivation | |
| CN107148880A (en) | A kind of implantation methods of high calcium organic rice | |
| CN105474980A (en) | Planting method of greenhouse potatoes | |
| CN102986437A (en) | Method for cultivating radix zanthoxyli artificially | |
| CN108157115B (en) | Compound planting method of paeonia rockii for oil in arid sand area | |
| CN107912238A (en) | The organic implantation methods of banana good quality and high output | |
| CN110235731A (en) | A method of substitution control being carried out to field Alternanthera philoxeroides using sweet potato | |
| CN102027855A (en) | Technology for processing coastal glehnia root medicinal materials in planting and producing area and quality standard | |
| CN106069151B (en) | High-yield planting method for coix as pasture | |
| CN105961122A (en) | Longan and sweet potato interplanting method | |
| Ekanayake et al. | Agronomy of cassava | |
| CN110679423B (en) | Cultivation method for reducing hydrocyanic acid content of cassava | |
| CN100388876C (en) | Five-finger fig root standarded and industrilized seed breeding planting method | |
| CN104145650A (en) | Eucommia ulmoides planting method | |
| CN111108996A (en) | Cultivation method for layering clonal tea seedlings into high-density planted tea garden twice | |
| CN110679419A (en) | Substrate-based cultivation method for high-quality small sweet potato seedlings | |
| CN109169106A (en) | A kind of normalized planting method of Paris polyphylla good quality and high output | |
| Anitha et al. | Production technology of some major and minor spice crops | |
| CN113348939A (en) | Efficient three-dimensional planting and breeding combination method for peach trees, common fescue and golden cicadas | |
| CN111296153A (en) | Planting method for interplanting costus root under walnut forest | |
| Sharma et al. | MULBERRY-A multi purpose tree species for varied climate | |
| Jayachandran et al. | Advances in production technology of sapota | |
| CN107258278A (en) | A kind of peanut cultivation method | |
| CN1473458A (en) | Cultivation and breeding method for Adonis amurensis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20200114 Assignee: Guangxi Hongge Biotechnology Co.,Ltd. Assignor: GUANGXI ACADEMY OF AGRICULTURAL SCIENCES Contract record no.: X2023980044722 Denomination of invention: A cultivation method for reducing the content of hydrocyanic acid in cassava Granted publication date: 20210727 License type: Common License Record date: 20231030 Application publication date: 20200114 Assignee: Guangxi Nongken Mingyang starch Development Co.,Ltd. Assignor: GUANGXI ACADEMY OF AGRICULTURAL SCIENCES Contract record no.: X2023980044712 Denomination of invention: A cultivation method for reducing the content of hydrocyanic acid in cassava Granted publication date: 20210727 License type: Common License Record date: 20231031 |
|
| EE01 | Entry into force of recordation of patent licensing contract | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20200114 Assignee: Guangxi Fuchuan Dexin Ecological Agriculture Development Co.,Ltd. Assignor: GUANGXI ACADEMY OF AGRICULTURAL SCIENCES Contract record no.: X2023980046559 Denomination of invention: A cultivation method for reducing the content of hydrocyanic acid in cassava Granted publication date: 20210727 License type: Common License Record date: 20231108 Application publication date: 20200114 Assignee: Guilin Guanyang SHANGNONG Agricultural Development Co.,Ltd. Assignor: GUANGXI ACADEMY OF AGRICULTURAL SCIENCES Contract record no.: X2023980046558 Denomination of invention: A cultivation method for reducing the content of hydrocyanic acid in cassava Granted publication date: 20210727 License type: Common License Record date: 20231108 Application publication date: 20200114 Assignee: Guangxi Shunteng Agricultural Products Co.,Ltd. Assignor: GUANGXI ACADEMY OF AGRICULTURAL SCIENCES Contract record no.: X2023980046557 Denomination of invention: A cultivation method for reducing the content of hydrocyanic acid in cassava Granted publication date: 20210727 License type: Common License Record date: 20231108 Application publication date: 20200114 Assignee: Guangxi Hezhou Funong Ecological Agriculture Co.,Ltd. Assignor: GUANGXI ACADEMY OF AGRICULTURAL SCIENCES Contract record no.: X2023980046556 Denomination of invention: A cultivation method for reducing the content of hydrocyanic acid in cassava Granted publication date: 20210727 License type: Common License Record date: 20231108 Application publication date: 20200114 Assignee: Fuchuan Taro Union Planting and Breeding Professional Cooperative Assignor: GUANGXI ACADEMY OF AGRICULTURAL SCIENCES Contract record no.: X2023980046561 Denomination of invention: A cultivation method for reducing the content of hydrocyanic acid in cassava Granted publication date: 20210727 License type: Common License Record date: 20231108 |
|
| EE01 | Entry into force of recordation of patent licensing contract |